RU97105592A - METHOD FOR QUANTITATIVE DETERMINATION OF AUTOANTIBODIES TO LOW DENSITY LIPOPROTEIDS IN BLOOD SERUM - Google Patents

METHOD FOR QUANTITATIVE DETERMINATION OF AUTOANTIBODIES TO LOW DENSITY LIPOPROTEIDS IN BLOOD SERUM

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Publication number
RU97105592A
RU97105592A RU97105592/14A RU97105592A RU97105592A RU 97105592 A RU97105592 A RU 97105592A RU 97105592/14 A RU97105592/14 A RU 97105592/14A RU 97105592 A RU97105592 A RU 97105592A RU 97105592 A RU97105592 A RU 97105592A
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RU
Russia
Prior art keywords
autoantibodies
ldl
low density
blood serum
quantitative determination
Prior art date
Application number
RU97105592/14A
Other languages
Russian (ru)
Other versions
RU2137134C1 (en
Inventor
В.Н. Хлюстов
Original Assignee
Учебно-научный центр Медицинского центра при Правительстве РФ
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Publication date
Application filed by Учебно-научный центр Медицинского центра при Правительстве РФ filed Critical Учебно-научный центр Медицинского центра при Правительстве РФ
Priority to RU97105592A priority Critical patent/RU2137134C1/en
Priority claimed from RU97105592A external-priority patent/RU2137134C1/en
Publication of RU97105592A publication Critical patent/RU97105592A/en
Application granted granted Critical
Publication of RU2137134C1 publication Critical patent/RU2137134C1/en

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Claims (1)

Способ количественного определения аутоантител к липопротеидам низкой плотности в сыворотке крови путем проведения твердофазного иммуноферментного анализа, отличающийся тем, что выполняют стандартизацию по аффинным аутоантителам к ЛПНП с известной концентрацией белка легких цепей иммуноглибулинов, причем, в качестве связывающего агента используют ЛПНП, выделенные от большого числа доноров и очищенные от циркулирующих иммунных комплексов "ЛПНП+аутоантитело", а в качестве вторых антител для обнаружения аутоантител к ЛПНП в иммуноферментной реакции используют козьи поликлональные антитела к легким цепям иммуноглобулинов человека меченные пероксидазой.A method for the quantitative determination of autoantibodies to low density lipoproteins in blood serum by conducting an enzyme-linked immunosorbent assay, characterized in that they perform standardization for affinity autoantibodies to LDL with a known concentration of immunoglybulin light chain protein, and LDL isolated from a large number of donors is used as a binding agent and purified from circulating immune complexes "LDL + autoantibody", and as second antibodies for the detection of autoantibodies to LDL in the immunofer The reaction uses goat polyclonal antibodies to the light chains of human immunoglobulins labeled with peroxidase.
RU97105592A 1997-04-15 1997-04-15 Method of quantitative determination of autoantibodies to blood serum low density lipoproteins RU2137134C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU97105592A RU2137134C1 (en) 1997-04-15 1997-04-15 Method of quantitative determination of autoantibodies to blood serum low density lipoproteins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU97105592A RU2137134C1 (en) 1997-04-15 1997-04-15 Method of quantitative determination of autoantibodies to blood serum low density lipoproteins

Publications (2)

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RU97105592A true RU97105592A (en) 1999-04-20
RU2137134C1 RU2137134C1 (en) 1999-09-10

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RU97105592A RU2137134C1 (en) 1997-04-15 1997-04-15 Method of quantitative determination of autoantibodies to blood serum low density lipoproteins

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2623879C2 (en) * 2015-11-25 2017-06-29 Федеральное государственное бюджетное научное учреждение "Научно-исследовательский институт общей патологии и патофизиологии" Method for circulating desialylated low density lipoproteids content estimation

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