RU96112671A - METHOD AND KIT FOR DETECTION OF ANTIBODIES TO RIFT VALUE FEVER VIRUS IN ANIMAL SERUM - Google Patents

METHOD AND KIT FOR DETECTION OF ANTIBODIES TO RIFT VALUE FEVER VIRUS IN ANIMAL SERUM

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Publication number
RU96112671A
RU96112671A RU96112671/13A RU96112671A RU96112671A RU 96112671 A RU96112671 A RU 96112671A RU 96112671/13 A RU96112671/13 A RU 96112671/13A RU 96112671 A RU96112671 A RU 96112671A RU 96112671 A RU96112671 A RU 96112671A
Authority
RU
Russia
Prior art keywords
streptavidin
fever virus
biotin
kit
rift
Prior art date
Application number
RU96112671/13A
Other languages
Russian (ru)
Other versions
RU2112245C1 (en
Inventor
Л.Ю. Вергун
И.Ф. Вишняков
Original Assignee
Всероссийский научно-исследовательский институт ветеринарной вирусологии и микробиологии
Filing date
Publication date
Application filed by Всероссийский научно-исследовательский институт ветеринарной вирусологии и микробиологии filed Critical Всероссийский научно-исследовательский институт ветеринарной вирусологии и микробиологии
Priority to RU96112671A priority Critical patent/RU2112245C1/en
Priority claimed from RU96112671A external-priority patent/RU2112245C1/en
Application granted granted Critical
Publication of RU2112245C1 publication Critical patent/RU2112245C1/en
Publication of RU96112671A publication Critical patent/RU96112671A/en

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Claims (2)

1. Способ обнаружения антител к вирусу лихорадки долины Рифт в сыворотках животных, включающий постановку реакции и учет твердофазного иммуноферментного анализа, отличающийся тем, что проводят твердофазный иммуноферментный анализ на основе стрептавидин-биотинового комплекса, исследуемые сыворотки овец вносят в лунки микротитрационных пластин, предварительно сенсибилизированные рабочей дозой специфического антигена, и сорбируют, далее инкубацию проводят с конъюгатами в виде стрептавидинбиотинового комплекса, включающего антиовечьи IgJ-биотин + стрептавидин + биотин - β-галактозидазу в соотношении стрептавидина и биотин - β - галактозидазы 2 : 1 или 1 : 1.1. A method for detecting antibodies to the Rift Valley fever virus in animal sera, including setting up the reaction and taking the enzyme-linked immunosorbent assay, characterized in that the enzyme-linked immunosorbent assay is performed on the basis of the streptavidin-biotin complex, the studied sheep sera are introduced into the wells of microtiter plates, previously sensitized dose of a specific antigen, and sorb, then incubation is carried out with conjugates in the form of streptavidin-biotin complex, including anti-infusion and IgJ-biotin + streptavidin + biotin-β-galactosidase in the ratio of streptavidin and biotin-β-galactosidase 2: 1 or 1: 1. 2. Набор для обнаружения антител к вирусу лихорадки долины Рифт, содержащий специфический антиген вируса лихорадки долины Рифт, контрольную сыворотку от клинически здоровой овцы, специфическую сыворотку к вирусу ЛДР, глобулиновый конъюгат, отличающийся тем, что набор содержит конъюгат в виде комплекса антиовечьего биотинилированного IgJ, стрептавидина и биотинилированной β-галактозидазы. 2. A kit for detecting antibodies to Rift Valley fever virus, containing a specific Rift Valley fever virus antigen, a control serum from a clinically healthy sheep, a specific serum to LDR virus, a globulin conjugate, characterized in that the kit contains a conjugate in the form of a complex of anti-biotinylated IgJ, streptavidin and biotinylated β-galactosidase.
RU96112671A 1996-06-21 1996-06-21 Test set and method for detecting rift valley fever virus antibodies in animal blood serum RU2112245C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU96112671A RU2112245C1 (en) 1996-06-21 1996-06-21 Test set and method for detecting rift valley fever virus antibodies in animal blood serum

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU96112671A RU2112245C1 (en) 1996-06-21 1996-06-21 Test set and method for detecting rift valley fever virus antibodies in animal blood serum

Publications (2)

Publication Number Publication Date
RU2112245C1 RU2112245C1 (en) 1998-05-27
RU96112671A true RU96112671A (en) 1998-09-20

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU96112671A RU2112245C1 (en) 1996-06-21 1996-06-21 Test set and method for detecting rift valley fever virus antibodies in animal blood serum

Country Status (1)

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RU (1) RU2112245C1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2457255C2 (en) * 2010-06-01 2012-07-27 Государственное научное учреждение Всероссийский научно-исследовательский институт ветеринарной вирусологии и микробиологии Россельхозакадемии Synthetic oligonucleotide primers, method for detecting and differentiating rift valley virus strains by polymerase chain reaction and restriction enzyme digest analysis
CN107478838A (en) * 2017-09-25 2017-12-15 贵州省人民医院 A kind of colloidal gold immuno-chromatography test paper strip for detecting people and the anti-RVF antiviral antibodies of animal blood serum

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