RU95116325A - Strain of bacterium alcaligenes species - a producer of nitrilase - Google Patents

Strain of bacterium alcaligenes species - a producer of nitrilase

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Publication number
RU95116325A
RU95116325A RU95116325/13A RU95116325A RU95116325A RU 95116325 A RU95116325 A RU 95116325A RU 95116325/13 A RU95116325/13 A RU 95116325/13A RU 95116325 A RU95116325 A RU 95116325A RU 95116325 A RU95116325 A RU 95116325A
Authority
RU
Russia
Prior art keywords
strain
acrylonitrile
activity
nitrilase
alcaligenes
Prior art date
Application number
RU95116325/13A
Other languages
Russian (ru)
Other versions
RU2081169C1 (en
Inventor
Е.В. Забазная
С.В. Козулин
Л.К. Куликова
С.П. Воронин
Original Assignee
Саратовский филиал Государственного научно-исследовательского института генетики и селекции промышленных микроорганизмов
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Publication date
Application filed by Саратовский филиал Государственного научно-исследовательского института генетики и селекции промышленных микроорганизмов filed Critical Саратовский филиал Государственного научно-исследовательского института генетики и селекции промышленных микроорганизмов
Priority to RU9595116325A priority Critical patent/RU2081169C1/en
Publication of RU95116325A publication Critical patent/RU95116325A/en
Application granted granted Critical
Publication of RU2081169C1 publication Critical patent/RU2081169C1/en

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Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

FIELD: microbiological industry, biotechnology. SUBSTANCE: strain Alcaligenes species ВКПМ B-6706 shows high nitrilase activity that is 915, 134.7 and 58.5 mM/g x hr for acrylonitrile, propionitrile and butyronitrile, respectively. Strain shows activity at the range of pH = 4-9.5, optimal value pH = 8.0. Temperature range of nitrilase activity in cells of strain Alcaligenes sp. Iв-3 ВКПМ B-6706 is 2-65 C, maximal cell activity for acrylonitrile - at 50 C. Strain does not depend on toxic nitriles in cultivation medium. Strain Alcaligenes species ВКПМ B-6706 can be used in biotechnological process of preparing acrylic and other carboxylic acids. Strain is isolated from soil of acrylonitrile production. EFFECT: preparing strain indicated above, high nitrilase activity. 3 tbl

Claims (1)

Сущность изобретения: получение нового штамма бактерий - продуцента фермента нитрилазы, выделенного из почвы производства акрилонитрила методом накопительной культуры с использованием в качестве селектирующего агента ацетонитрила. По совокупности морфологических, культуральных, биохимических признаков штамм отнесен к роду Alcaligenes. Штамм Alcaligenes species ВКПМ В-6706 обладает высокой активностью нитрилазы, достигающей 915 mМ/г•ч в отношении акрилонитрила, 134.7 mМ/г•ч в отношении пропионитрила и 58.5 mМ/г•ч в отношении бутиронитрила. Штамм проявляет ферментативную активность в интервале рН 4 - 9,5, оптимальное значение рН 8,0. Температурный диапазон проявления нитрилазной активности клетками штамма Alcaligenes sp. 1в-3 ВКПМ В-6706 составляет 2 - 65°С, максимальная активность клеток в отношении акрилонитрила проявляется при 50oС. Штамм не требует внесения в среду культивирования токсичных нитрилов. Штамм Alcaligenes species ВКПМ В-6706 может быть использован в биотехнологическом процессе получения акриловой и других карбоновых кислот.The essence of the invention: obtaining a new strain of bacteria - a producer of the nitrilase enzyme isolated from the soil of the production of acrylonitrile by the method of cumulative culture using acetonitrile as a selection agent. According to the combination of morphological, cultural, biochemical characters, the strain is assigned to the genus Alcaligenes. The strain Alcaligenes species VKPM B-6706 has a high nitrilase activity, reaching 915 mM / g • h in relation to acrylonitrile, 134.7 mM / g • h in relation to propionitrile and 58.5 mM / g • h in relation to butyronitrile. The strain exhibits enzymatic activity in the range of pH 4 - 9.5, the optimum pH value of 8.0. The temperature range for the manifestation of nitrilase activity by cells of the strain Alcaligenes sp. 1c-3 VKPM B-6706 is 2 - 65 ° C, the maximum activity of cells against acrylonitrile appears at 50 o C. The strain does not require the introduction of toxic nitriles into the culture medium. The strain Alcaligenes species VKPM B-6706 can be used in the biotechnological process of producing acrylic and other carboxylic acids.
RU9595116325A 1995-09-19 1995-09-19 Strain of bacterium alcaligenes species - producer of nitrilase RU2081169C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU9595116325A RU2081169C1 (en) 1995-09-19 1995-09-19 Strain of bacterium alcaligenes species - producer of nitrilase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU9595116325A RU2081169C1 (en) 1995-09-19 1995-09-19 Strain of bacterium alcaligenes species - producer of nitrilase

Publications (2)

Publication Number Publication Date
RU95116325A true RU95116325A (en) 1996-08-10
RU2081169C1 RU2081169C1 (en) 1997-06-10

Family

ID=20172252

Family Applications (1)

Application Number Title Priority Date Filing Date
RU9595116325A RU2081169C1 (en) 1995-09-19 1995-09-19 Strain of bacterium alcaligenes species - producer of nitrilase

Country Status (1)

Country Link
RU (1) RU2081169C1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2177034C1 (en) * 2001-04-03 2001-12-20 Закрытое акционерное общество "Биоамид" Strain of bacterium alcaligenes denitrificans as nitrilase producer

Also Published As

Publication number Publication date
RU2081169C1 (en) 1997-06-10

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