RU95114535A - GENE encoding carminomycin-4-0-methyltransferase, vector, host cell and method for producing daunorubicin - Google Patents

GENE encoding carminomycin-4-0-methyltransferase, vector, host cell and method for producing daunorubicin

Info

Publication number
RU95114535A
RU95114535A RU95114535/13A RU95114535A RU95114535A RU 95114535 A RU95114535 A RU 95114535A RU 95114535/13 A RU95114535/13 A RU 95114535/13A RU 95114535 A RU95114535 A RU 95114535A RU 95114535 A RU95114535 A RU 95114535A
Authority
RU
Russia
Prior art keywords
vector
methyltransferase
carminomycin
plasmid
host cell
Prior art date
Application number
RU95114535/13A
Other languages
Russian (ru)
Other versions
RU2155811C2 (en
Inventor
Ричард Хатчинсон Чарльз
Марти Маддури Кришна
Торти Франческа
Луиза Коломбо Анна
Original Assignee
Фармация Энд Апджон С.П.А.
Ричард Хатчинсон Чарльз
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Фармация Энд Апджон С.П.А., Ричард Хатчинсон Чарльз filed Critical Фармация Энд Апджон С.П.А.
Publication of RU95114535A publication Critical patent/RU95114535A/en
Application granted granted Critical
Publication of RU2155811C2 publication Critical patent/RU2155811C2/en

Links

Claims (13)

1. Выделенная ДНК-последовательность, содержащая ген, который кодирует карминомицин-4-О-метилтрансферазу.1. An isolated DNA sequence containing a gene that encodes carminomycin-4-O-methyltransferase. 2. ДНК-последовательность по п.1, отличающаяся тем, что содержит всю или часть ДНК, представленную на с.185. 2. The DNA sequence according to claim 1, characterized in that it contains all or part of the DNA presented on p. 3. Вектор, содержащий ген, который кодирует карминомицин-4-О-метилтрансферазу. 3. A vector containing a gene that encodes carminomycin-4-O-methyltransferase. 4. Вектор по п.3, отличающийся тем, что представляет плазмиду. 4. The vector according to claim 3, characterized in that it is a plasmid. 5. Плазмида по п.4, отличающаяся тем, что ее выбирают из группы, состоящей из pW HM901, pW HM902 и pW HM903. 5. The plasmid according to claim 4, characterized in that it is selected from the group consisting of pW HM901, pW HM902 and pW HM903. 6. Трансформированная хозяйская клетка, содержащая вектор, который включает ген, кодирующий карминомицин-4-О-метилтрансферазу. 6. A transformed host cell containing a vector that includes a gene encoding carminomycin-4-O-methyltransferase. 7. Клетка по п. 6, отличающаяся тем, что указанным вектором является плазмида. 7. A cell according to claim 6, characterized in that the vector is a plasmid. 8. Клетка по п.7, отличающаяся тем, что указанную хозяйскую клетку выбирают из группы, состоящей из E.coli и Streptomyces. 8. The cell according to claim 7, characterized in that said host cell is selected from the group consisting of E. coli and Streptomyces. 9. Клетка по п. 6, отличающаяся тем, что указанным вектором является плазмида, выбранная из группы, состоящей из pW HM901, pW HM902 и pW HM903. 9. The cell of claim 6, wherein said vector is a plasmid selected from the group consisting of pW HM901, pW HM902 and pW HM903. 10. Способ получения даунорубицина, отличающийся тем, что подходящие хозяйские клетки трансформируют с помощью вектора, содержащего ген, который кодирует карминомицин-4-0-метилтрансферазу, указанные хозяйские клетки культивируют в подходящей среде, стимулируют экспрессию указанного гена и выделяют даунорубицин. 10. A method for producing daunorubicin, characterized in that suitable host cells are transformed using a vector containing a gene that encodes carminomycin-4-0-methyltransferase, these host cells are cultured in a suitable medium, stimulate the expression of the gene and secrete daunorubicin. 11. Способ по п.10, отличающийся тем, что хозяйскими клетками являются клетки Streptomyces. 11. The method according to claim 10, characterized in that the host cells are Streptomyces cells. 12. Способ по п.10, отличающийся тем, что вектором является плазмида. 12. The method according to claim 10, characterized in that the vector is a plasmid. 13. Способ по п.12, отличающийся тем, что плазмиду выбирают из группы, состоящей из pW HM901, pW HM902 и pW HM903. 13. The method according to p. 12, characterized in that the plasmid is selected from the group consisting of pW HM901, pW HM902 and pW HM903.
RU95114535/13A 1991-11-18 1992-11-17 Gene encoding carminomycin-4-o-methyltransferase, vector, host cell and method of daunorubicin producing RU2155811C2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US79387391A 1991-11-18 1991-11-18
US07/793,873 1991-11-18
US07/959,941 1992-10-09

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
RU93054774A Division RU2115731C1 (en) 1991-11-18 1992-11-17 Polypeptide showing carminomycin-4-o-methyltransferase activity and a method of its preparing

Publications (2)

Publication Number Publication Date
RU95114535A true RU95114535A (en) 1998-05-27
RU2155811C2 RU2155811C2 (en) 2000-09-10

Family

ID=25161036

Family Applications (2)

Application Number Title Priority Date Filing Date
RU93054774A RU2115731C1 (en) 1991-11-18 1992-11-17 Polypeptide showing carminomycin-4-o-methyltransferase activity and a method of its preparing
RU95114535/13A RU2155811C2 (en) 1991-11-18 1992-11-17 Gene encoding carminomycin-4-o-methyltransferase, vector, host cell and method of daunorubicin producing

Family Applications Before (1)

Application Number Title Priority Date Filing Date
RU93054774A RU2115731C1 (en) 1991-11-18 1992-11-17 Polypeptide showing carminomycin-4-o-methyltransferase activity and a method of its preparing

Country Status (7)

Country Link
US (1) US5364781A (en)
KR (1) KR100283826B1 (en)
PT (1) PT101065B (en)
RU (2) RU2115731C1 (en)
TW (1) TW227575B (en)
UA (1) UA39922C2 (en)
ZA (1) ZA928865B (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ245124A (en) * 1991-11-18 1994-06-27 Erba Carlo Spa Carminomycin 4-0-methyltransferase, dna encoding it, vectors and host cells; and also a process for producing daunorubicin
FI944556A0 (en) * 1994-09-30 1994-09-30 Kristiina Ylihonko Foerfarande Foer producering av anthracycliner and deras mellanprodukter
GB2302540B (en) * 1995-06-23 1999-02-24 Pharmacia Spa Cofactor for transmethylation
US5652125A (en) * 1996-06-10 1997-07-29 Pharmacia S.P.A. Process for preparing daunorubicin
DE19880405T1 (en) * 1997-03-06 1999-11-11 Pharmacia & Upjohn Spa Process for the preparation of doxorubicin
CN1198930C (en) * 1998-04-24 2005-04-27 法玛西雅厄普约翰公司 Process for preparing doxorubicin
US6177262B1 (en) * 1998-09-22 2001-01-23 Kosan Biosciences, Inc. Recombinant host cells for the production of polyketides
KR102080666B1 (en) * 2019-04-12 2020-02-24 박행제 Turbine assemblies for Hydroelectric power devices

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