RU95110283A - Method of bacterium identification
- Google Patents
Method of bacterium identification
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Publication number
RU95110283A
RU95110283ARU95110283/13ARU95110283ARU95110283ARU 95110283 ARU95110283 ARU 95110283ARU 95110283/13 ARU95110283/13 ARU 95110283/13ARU 95110283 ARU95110283 ARU 95110283ARU 95110283 ARU95110283 ARU 95110283A
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Application filed by С.Д. Колпакова, А.И. КолпаковfiledCriticalС.Д. Колпакова
Priority to RU95110283ApriorityCriticalpatent/RU2113467C1/en
Publication of RU95110283ApublicationCriticalpatent/RU95110283A/en
Application grantedgrantedCritical
Publication of RU2113467C1publicationCriticalpatent/RU2113467C1/en
Measuring Or Testing Involving Enzymes Or Micro-Organisms
(AREA)
Abstract
FIELD: microbiology, medicine. SUBSTANCE: method is based on determination of diffusion coefficient of bacterial cells in liquid medium, i. e. concentration gradient disappearance time at the known fixed volume of liquid medium and inoculate added to it. Diffusion coefficient can be used for identification both live and killed bacterial cells. Analysis time is 15-60 min. Method can be used for laboratory diagnosis of infectious diseases and microbiological examination of environment objects. EFFECT: accelerated method of identification.
Claims (1)
Изобретение относится к микробиологии и может быть использован при лабораторной диагностике инфекционных заболеваний, микробиологическим исследовании объектов окружающей среды. Изобретение позволяет в течение 15 - 60 мин провести идентификацию бактерий. За критерий идентификации в данном способе принимается коэффициент диффузии бактериальных клеток в жидкой среде, т. е. время исчезновения градиента концентрации при заданном фиксированном объеме жидкой среды и вносимого в нее инокулята. Причем коэффициент диффузии можно использовать в качестве критерия идентификации как живых, так и убитых бактериальных клеток.The invention relates to microbiology and can be used in laboratory diagnosis of infectious diseases, microbiological examination of environmental objects. The invention allows for identification of bacteria within 15-60 minutes. The identification criterion in this method is the diffusion coefficient of bacterial cells in a liquid medium, i.e., the time of disappearance of the concentration gradient at a given fixed volume of the liquid medium and the inoculum introduced into it. Moreover, the diffusion coefficient can be used as a criterion for the identification of both living and dead bacterial cells.
RU95110283A1995-06-191995-06-19Method of bacterium identification
RU2113467C1
(en)