RU95101339A - Method of anthrax microbe strains differentiation in vitro by virulence - Google Patents

Method of anthrax microbe strains differentiation in vitro by virulence

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Publication number
RU95101339A
RU95101339A RU95101339/13A RU95101339A RU95101339A RU 95101339 A RU95101339 A RU 95101339A RU 95101339/13 A RU95101339/13 A RU 95101339/13A RU 95101339 A RU95101339 A RU 95101339A RU 95101339 A RU95101339 A RU 95101339A
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RU
Russia
Prior art keywords
virulent
differentiation
vitro
strains
groups
Prior art date
Application number
RU95101339/13A
Other languages
Russian (ru)
Other versions
RU2101351C1 (en
Inventor
Е.И. Еременко
Н.П. Буравцева
Т.Н. Фунтикова
Original Assignee
Ставропольский научно-исследовательский противочумный институт
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Application filed by Ставропольский научно-исследовательский противочумный институт filed Critical Ставропольский научно-исследовательский противочумный институт
Priority to RU95101339A priority Critical patent/RU2101351C1/en
Publication of RU95101339A publication Critical patent/RU95101339A/en
Application granted granted Critical
Publication of RU2101351C1 publication Critical patent/RU2101351C1/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

FIELD: medicinal microbiology. SUBSTANCE: method involves the use of index of capsule formation and, additionally, toxin formation in vitro. Capsule and toxin production is determined on agar simultaneously for immunological examination. Method ensures to differentiate strains for 5 groups: high virulent, moderate virulent, weak virulent, nonvirulent and nonpathogenic while the known method ensures to detect only two groups: virulent and nonvirulent ones. EFFECT: increased precision of method.

Claims (1)

Изобретение относится к медицинской микробиологии, в частности к дифференциации штаммов по вирулентности, и может быть использовано для эпидемиологического и эпизоотологического анализа при сибирской язве и для внутривидовой таксономии Bacillus anthracis. Цель: повышение точности способа дифференциации штаммов возбудителя сибирской язвы. Сущность изобретения: в качестве дополнительного критерия для дифференциации наряду с капсулообразованием in vitro используется токсинообразование, продукция капсулы и токсина определяется одновременно на агаре для иммунологического исследования, что позволяет дифференцировать штаммы на 5 групп: высоковирулентные, умеренновирулентные, слабовирулентные, авирулентные и апатогенные, в отличие от существующего способа, выделяющего только две группы: вирулентные и авирулентные.The invention relates to medical microbiology, in particular to the differentiation of strains by virulence, and can be used for epidemiological and epizootological analysis for anthrax and for intraspecific taxonomy of Bacillus anthracis. Purpose: improving the accuracy of the method of differentiation of anthrax pathogen strains. The invention: as an additional criterion for differentiation, along with capsule formation in vitro, toxin formation is used, capsule and toxin production is determined simultaneously on agar for immunological studies, which allows differentiating the strains into 5 groups: highly virulent, moderately virulent, weakly virulent, avirulent and apatogenic, in contrast the existing method, distinguishing only two groups: virulent and avirulent.
RU95101339A 1995-01-31 1995-01-31 Method of differentiation of anthrax microbe strains by virulence in vitro RU2101351C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU95101339A RU2101351C1 (en) 1995-01-31 1995-01-31 Method of differentiation of anthrax microbe strains by virulence in vitro

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU95101339A RU2101351C1 (en) 1995-01-31 1995-01-31 Method of differentiation of anthrax microbe strains by virulence in vitro

Publications (2)

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RU95101339A true RU95101339A (en) 1997-03-20
RU2101351C1 RU2101351C1 (en) 1998-01-10

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RU95101339A RU2101351C1 (en) 1995-01-31 1995-01-31 Method of differentiation of anthrax microbe strains by virulence in vitro

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RU2101351C1 (en) 1998-01-10

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RU95101339A (en) Method of anthrax microbe strains differentiation in vitro by virulence
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