RU94042470A - Polyvalent vaccine for mareck's disease prophylaxis and method of its preparing - Google Patents

Polyvalent vaccine for mareck's disease prophylaxis and method of its preparing

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Publication number
RU94042470A
RU94042470A RU94042470/13A RU94042470A RU94042470A RU 94042470 A RU94042470 A RU 94042470A RU 94042470/13 A RU94042470/13 A RU 94042470/13A RU 94042470 A RU94042470 A RU 94042470A RU 94042470 A RU94042470 A RU 94042470A
Authority
RU
Russia
Prior art keywords
serotype
vaccine
medium
preparing
amount
Prior art date
Application number
RU94042470/13A
Other languages
Russian (ru)
Other versions
RU2059414C1 (en
Inventor
В.А. Лукина
Ф.Г. Праксин
Д.И. Белый
Н.С. Шевырев
В.М. Безгин
А.А. Гусев
Е.В. Баррос-Палома
В.И. Смоленский
В.С. Панов
Original Assignee
Государственная курская биофабрика Фирма "Биок"
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Государственная курская биофабрика Фирма "Биок" filed Critical Государственная курская биофабрика Фирма "Биок"
Priority to RU94042470A priority Critical patent/RU2059414C1/en
Application granted granted Critical
Publication of RU2059414C1 publication Critical patent/RU2059414C1/en
Publication of RU94042470A publication Critical patent/RU94042470A/en

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Abstract

FIELD: veterinary science, virology. SUBSTANCE: invention claims polyvalent vaccine against Mareck's disease that contains turkey herpes virus (3-d serotype) as a basic virus and viruses of 2-d serotype (without preliminary adaptation and attenuation) as components enhancing immunogenesis isolated from healthy birds at amount 1-50% of basic one. Suspension of vaccine whole cells has the following media, wt.-%: Eagle medium or medium-199 80-90 and cattle serum blood 5-10 followed by addition of dimethylsulfoxide at amount 5-10 wt.-%. Method of preparing cell culture involves the separate infection of bird embryos with turkey herpes virus and one or more chicken herpes virus (2-d serotype) after 2-5 passages. EFFECT: enhanced effectiveness of vaccine, improved method of preparing.

Claims (1)

Предложена поливалентная вакцина против болезни Марека, которая в качестве основного (базового) вируса содержит вирус герпеса индейки 3-го серотипа, а в качестве усиливающих иммуногенность компонентов - вирусы 2-го серотипа (без предварительной адаптации и аттенуации), выделенные от птиц из благополучных хозяйств в количестве 1 - 50% от базового. В качестве среды суспензии целых клеток вакцина содержит, мас.%: среду Игла или среду 199 - 80 - 90, сыворотку крови крупного рогатого окота - 5 - 10, с последующим добавлением диметилсульфоксида в количестве 5 - 10 мас.%, По способу получения культуры клеток эмбрионов птиц раздельно инфицируют вирусами герпеса индейки и одним или более вирусами герпеса кур 2-го серотипа 2 - 5 пассажей.A multivalent vaccine is proposed against Marek’s disease, which contains the 3rd serotype of the herpes virus of the turkey of the 3rd serotype as the main (basic) virus, and the viruses of the 2nd serotype (without preliminary adaptation and attenuation) isolated from birds from the successful farms as immunogenicity enhancing components in an amount of 1 - 50% of the base. As a medium for a suspension of whole cells, the vaccine contains, wt.%: Medium Needle or medium 199 - 80 - 90, serum of cattle - 5 - 10, followed by the addition of dimethyl sulfoxide in an amount of 5 - 10 wt.%, According to the method of obtaining culture cells of bird embryos are separately infected with turkey herpes viruses and one or more chicken herpes viruses of the 2nd serotype 2-5 passages.
RU94042470A 1994-12-09 1994-12-09 Multipartial vaccine for mareck disease prophylaxis and a method of its preparing RU2059414C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU94042470A RU2059414C1 (en) 1994-12-09 1994-12-09 Multipartial vaccine for mareck disease prophylaxis and a method of its preparing

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU94042470A RU2059414C1 (en) 1994-12-09 1994-12-09 Multipartial vaccine for mareck disease prophylaxis and a method of its preparing

Publications (2)

Publication Number Publication Date
RU2059414C1 RU2059414C1 (en) 1996-05-10
RU94042470A true RU94042470A (en) 1996-08-20

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU94042470A RU2059414C1 (en) 1994-12-09 1994-12-09 Multipartial vaccine for mareck disease prophylaxis and a method of its preparing

Country Status (1)

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RU (1) RU2059414C1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2719409T5 (en) * 2011-11-30 2022-12-07 Boehringer Ingelheim Animal Health Usa Inc Recombinant Gallid herpesvirus 3 (mdv serotype 2) vectors expressing avian pathogen antigens and uses thereof

Also Published As

Publication number Publication date
RU2059414C1 (en) 1996-05-10

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MM4A The patent is invalid due to non-payment of fees

Effective date: 20111210