RU94024845A - Improved method of differentiation of vaccine and pathogen strains of cattle brucelli
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Improved method of differentiation of vaccine and pathogen strains of cattle brucelli
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RU94024845A
RU94024845ARU94024845/13ARU94024845ARU94024845ARU 94024845 ARU94024845 ARU 94024845ARU 94024845/13 ARU94024845/13 ARU 94024845/13ARU 94024845 ARU94024845 ARU 94024845ARU 94024845 ARU94024845 ARU 94024845A
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Application filed by Казанский ветеринарный институт им.Н.Э.БауманаfiledCriticalКазанский ветеринарный институт им.Н.Э.Баумана
Priority to RU94024845ApriorityCriticalpatent/RU2095418C1/en
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Measuring Or Testing Involving Enzymes Or Micro-Organisms
(AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents
(AREA)
Abstract
FIELD: microbiology, molecular biology. SUBSTANCE: DNA of brucelli contains the unique repeated nucleotide sequences showing complementarity with that of synthetic oligonucleotide /CAC/.. Hypervariable sequences can be detected by digestion of brucella DNA with restriction enzyme NcoI followed by elecetrophoresis and hybridization with DNA-probe /CAC/.. Bands pattern (sites of unique nucleotide sequence) are specific for different brucella strains that ensures to differentiate thereof. EFFECT: increased safety and specificity of method for identification and differentiation of brucelli.
Claims (1)
Изобретение относится к области микробиологии, молекулярной биологии и касается способа дифференциации различных штаммов бруцелл вида Вrucel1а аbоrtus. Целью изобретения является повышение надежности и специфичности способа идентификации и дифференциации различных вакцинных штаммов бруцелл и возбудителя бруцеллеза крупного рогатого скота. Сущность изобретения состоит в том, что ДНК бруцелл содержит в своем составе уникальные повторяющиеся последовательности нуклеотидов. Они комплементарны к последовательности синтетического олигонуклеотида (САС) 5. Гипервариабельные последовательности обнаруживаются рестрикцией ДНК бруцелл ферментом Nсоl, электрофорезом их и гибридизацией ДНК-зондом (САС) 5. Расположения полос (участков уникальных последовательностей нуклеотидов) являются специфичными для различных штаммов бруцелл и позволяют дифференцировать их.The invention relates to the field of microbiology, molecular biology, and relates to a method for differentiation of various strains of Brucella species Brucel1a abortus. The aim of the invention is to increase the reliability and specificity of the method of identification and differentiation of various vaccine strains of brucella and the causative agent of brucellosis in cattle. The essence of the invention lies in the fact that Brucella DNA contains unique repeating nucleotide sequences. They are complementary to the sequence of synthetic oligonucleotide (CAC) 5. Hypervariable sequences are detected by restriction of brucella DNA with NcoL enzyme, electrophoresis of them and DNA probe hybridization (CAC) 5. The arrangement of bands (regions of unique nucleotide sequences) are specific for different strains of brucella and allow differentiating them .
RU94024845A1994-07-011994-07-01Method of cattle brucella differentiation
RU2095418C1
(en)
Nucleotide sequences useful as type-specific probes, PCR primers and LCR probes for amplifying and detecting human papillomaviruses, and related kits and methods