RU94021756A - Strain of bacterium bacillus subtilis - producer of riboflavin
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Strain of bacterium bacillus subtilis - producer of riboflavin
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RU94021756A
RU94021756ARU94021756/13ARU94021756ARU94021756ARU 94021756 ARU94021756 ARU 94021756ARU 94021756/13 ARU94021756/13 ARU 94021756/13ARU 94021756 ARU94021756 ARU 94021756ARU 94021756 ARU94021756 ARU 94021756A
Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов
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Preparation Of Compounds By Using Micro-Organisms
(AREA)
Micro-Organisms Or Cultivation Processes Thereof
(AREA)
Abstract
FIELD: biotechnology, vitamins. SUBSTANCE: proposed strain is obtained by addition of recombinant plasmid carrying riboflavin operon of the strain B. subtilis with damaged negative regulation in recipient strain of B. subtilis. Strain-producer is resistant to 8-azaguanine, methionine-sulfoxide, diacetyl, psicofuranin and erythromycin and ensures to obtain up to 12.4 g/l riboflavin for 42 hr cultivation. EFFECT: increased yield of riboflavin.
Claims (1)
Предлагается штамм - продуцент рибофлавина, полученный путем введения в реципиентный штамм Bacillus subtilis рекомбинантной плазмиды, несущей рибофлавиновый оперон штамма Bacillus subtilis с нарушенной негативной регуляцией. Штамм-продуцент устойчив к 8- азагуанину, метионин-сульфоксиду, диацетилу, псикофуранину и к эритромицину и позволяет получать до 12,4 г/л рибофлавина за 42 ч культивирования.A strain producing riboflavin is proposed, obtained by introducing into the recipient strain of Bacillus subtilis a recombinant plasmid carrying the riboflavin operon of the Bacillus subtilis strain with impaired negative regulation. The producer strain is resistant to 8-azaguanine, methionine sulfoxide, diacetyl, psikofuranin and erythromycin and allows to obtain up to 12.4 g / l of riboflavin in 42 hours of cultivation.
RU94021756A1994-06-151994-06-15Strain of bacterium bacillus subtilis - a producer of riboflavin
RU2081906C1
(en)
Учреждение Российской академии медицинских наук научно-исследовательский институт вакцин и сывороток им. И.И. Мечникова РАМН (НИИВС им. И.И. Мечникова РАМН)
Fluid nutrient medium for high-activity metabolite collection by bacillus pumilus 'pashkov' strain showing inhibitory action on bacteria, fungi and viruses
BIOLOGICALLY CULTURE OF THE MICROORGANISM STREPTOMYCES ROSEOSPORUS NRRL 15998 AND A PROCEDURE FOR PREPARING ANTIBIOTIC EFFECTIVE A-21978C COMPLEX OR ANY OR CERO 'C' C 'C' 'C' FACTORS 'CO' FACTORS 'CO'
A method for controlling the expression of foreign genes by controlling the sugar concentration of the medium and a method for producing the foreign gene products accordingly