RU94020983A - Method of detection and quantitative determination of antibodies in biological liquids - Google Patents

Method of detection and quantitative determination of antibodies in biological liquids

Info

Publication number
RU94020983A
RU94020983A RU94020983/14A RU94020983A RU94020983A RU 94020983 A RU94020983 A RU 94020983A RU 94020983/14 A RU94020983/14 A RU 94020983/14A RU 94020983 A RU94020983 A RU 94020983A RU 94020983 A RU94020983 A RU 94020983A
Authority
RU
Russia
Prior art keywords
antibodies
antigen
detection
insoluble carrier
biological liquids
Prior art date
Application number
RU94020983/14A
Other languages
Russian (ru)
Inventor
Галлати Харалд
Ch]
Original Assignee
Фрезениус АГ (DE)
Фрезениус АГ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Фрезениус АГ (DE), Фрезениус АГ filed Critical Фрезениус АГ (DE)
Publication of RU94020983A publication Critical patent/RU94020983A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

FIELD: medicine. SUBSTANCE: method of detection and quantitative determination of antibodies in biological liquids uses immunologic reaction, during which sought for antibodies are incubated with corresponding antigen, one part of which is coupled with water insoluble carrier and other part is provided with mark. After phases separation and determination of marks number in liquid or solid phase components of analyzed sample may bind marked antigen with water insoluble carrier non-specifically and so giving false positive results. To produce correct results the reaction is carried out and incubation takes place in presence of surplus of unbound and non-marked antigen and after phases separation definite number of marks is also taken into account during qualitative estimation or quantitative analysis of antibodies. The technique is in particular applicable for analysis human being antibodies to rabbit globulin and in the case rabbit globulin is antigen. EFFECT: improved detection and quantitative determination of antibodies in biological liquids.

Claims (1)

Предложен способ для обнаружения или количественного определения антител в биологических жидкостях посредством иммунологической реакции, при которой искомые антитела инкубируются с соответствующим антигеном, одна часть которого связывается с водонерастворимым носителем, а другая часть снабжается меткой; после разделения фаз и определения количества метки, или в жидкой, или в твердой фазе возможно, что компоненты анализируемого образца свяжут меченный антиген с водонерастворимым носителем неспецифически, давая неверные положительные результаты. Чтобы получить правильные результаты проводится та же самая реакция, но инкубация происходит в присутствии избытка несвязанного и немеченого антигена, и после разделения фаз, определенное количество метки также принимается во внимание при качественной оценке или количественном анализе антител. Методика, в частности, удобна для анализа человеческих антител к кроличьему глобулину, причем антигеном является кроличий глобулин.The proposed method for the detection or quantification of antibodies in biological fluids through an immunological reaction in which the desired antibodies are incubated with the corresponding antigen, one part of which binds to a water-insoluble carrier, and the other part is labeled; after phase separation and determination of the amount of label, either in the liquid or in the solid phase, it is possible that the components of the analyzed sample will bind the labeled antigen with a water-insoluble carrier non-specifically, giving false positive results. To get the correct results, the same reaction is carried out, but the incubation takes place in the presence of an excess of unbound and unlabeled antigen, and after phase separation, a certain amount of label is also taken into account in the qualitative evaluation or quantitative analysis of antibodies. The technique, in particular, is convenient for the analysis of human antibodies to rabbit globulin, and the rabbit globulin is an antigen.
RU94020983/14A 1992-08-03 1993-07-30 Method of detection and quantitative determination of antibodies in biological liquids RU94020983A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH2432/92 1992-08-03
CH243292 1992-08-03

Publications (1)

Publication Number Publication Date
RU94020983A true RU94020983A (en) 1996-11-27

Family

ID=4233675

Family Applications (1)

Application Number Title Priority Date Filing Date
RU94020983/14A RU94020983A (en) 1992-08-03 1993-07-30 Method of detection and quantitative determination of antibodies in biological liquids

Country Status (10)

Country Link
EP (1) EP0606455A1 (en)
JP (1) JPH07505479A (en)
AU (1) AU665271B2 (en)
CA (1) CA2120348A1 (en)
FI (1) FI941502A (en)
NO (1) NO941094D0 (en)
RU (1) RU94020983A (en)
TW (1) TW274582B (en)
WO (1) WO1994003808A1 (en)
ZA (1) ZA935616B (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4595661A (en) * 1983-11-18 1986-06-17 Beckman Instruments, Inc. Immunoassays and kits for use therein which include low affinity antibodies for reducing the hook effect
US4788138A (en) * 1987-04-30 1988-11-29 Beckman Instruments, Inc. Method to achieve a linear standard curve in a sandwich immunoassay
US5223400A (en) * 1989-06-01 1993-06-29 The Ontario Cancer Institute Immunoassay method for determining the specificity of binding of a monoclonal antibody to an antigen

Also Published As

Publication number Publication date
NO941094L (en) 1994-03-25
AU665271B2 (en) 1995-12-21
AU4705393A (en) 1994-03-03
EP0606455A1 (en) 1994-07-20
JPH07505479A (en) 1995-06-15
FI941502A0 (en) 1994-03-30
ZA935616B (en) 1994-02-03
WO1994003808A1 (en) 1994-02-17
NO941094D0 (en) 1994-03-25
CA2120348A1 (en) 1994-02-17
TW274582B (en) 1996-04-21
FI941502A (en) 1994-05-26

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