RU93054302A - RECOMBINANT LGG-BINDING G PROTEIN G STREPTOCOKK GROUP G - Google Patents

RECOMBINANT LGG-BINDING G PROTEIN G STREPTOCOKK GROUP G

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Publication number
RU93054302A
RU93054302A RU93054302/14A RU93054302A RU93054302A RU 93054302 A RU93054302 A RU 93054302A RU 93054302/14 A RU93054302/14 A RU 93054302/14A RU 93054302 A RU93054302 A RU 93054302A RU 93054302 A RU93054302 A RU 93054302A
Authority
RU
Russia
Prior art keywords
protein
binding
group
streptocokk
recombinant
Prior art date
Application number
RU93054302/14A
Other languages
Russian (ru)
Other versions
RU2056859C1 (en
Inventor
Т.В. Гупалова
А.А. Тотолян
Original Assignee
Т.В. Гупалова
А.А. Тотолян
Filing date
Publication date
Application filed by Т.В. Гупалова, А.А. Тотолян filed Critical Т.В. Гупалова
Priority to RU93054302A priority Critical patent/RU2056859C1/en
Priority claimed from RU93054302A external-priority patent/RU2056859C1/en
Application granted granted Critical
Publication of RU2056859C1 publication Critical patent/RU2056859C1/en
Publication of RU93054302A publication Critical patent/RU93054302A/en

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Claims (1)

Рекомбинатный IgG связывающий G белок стрептококка группы G относится к медицине и медицинской биотехнологии и может найти применение для очистки иммуноглобулинов и использоваться в медицинской диагностике. Сущностью изобретения является получение IgG связывающего G белка путем клонирования гена G белка из стрептококка G 148 в E. coli, выделение белка из клеток Е.coli и очистка G белка посредством аффинной хроматографии с получением гомогенного белка, характеризующегося большим выходом и высокими константами связывания. Преимуществом изобретения является большой выход белка, гомогенность полученного G белка, высокая его аффинность и в связи с этим перспектива более успешного применения G белка в иммунологии, иммунохимии и в медицинской диагностике.Recombinant IgG G-binding protein of group G streptococcus belongs to medicine and medical biotechnology and can be used for purification of immunoglobulins and used in medical diagnostics. The essence of the invention is the production of IgG binding G protein by cloning the G gene of the protein from streptococcus G 148 into E. coli, isolating the protein from E. coli cells and purification of the G protein by affinity chromatography to obtain a homogeneous protein with a high yield and high binding constants. An advantage of the invention is the large yield of protein, the homogeneity of the obtained G protein, its high affinity and, therefore, the prospect of a more successful use of the G protein in immunology, immunochemistry and medical diagnostics.
RU93054302A 1993-12-14 1993-12-14 Recombinant igg-binding g-protein of streptococcus RU2056859C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU93054302A RU2056859C1 (en) 1993-12-14 1993-12-14 Recombinant igg-binding g-protein of streptococcus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU93054302A RU2056859C1 (en) 1993-12-14 1993-12-14 Recombinant igg-binding g-protein of streptococcus

Publications (2)

Publication Number Publication Date
RU2056859C1 RU2056859C1 (en) 1996-03-27
RU93054302A true RU93054302A (en) 1996-11-10

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU93054302A RU2056859C1 (en) 1993-12-14 1993-12-14 Recombinant igg-binding g-protein of streptococcus

Country Status (1)

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RU (1) RU2056859C1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2572343C2 (en) * 2014-04-15 2016-01-10 Федеральное государственное бюджетное научное учреждение "Институт экспериментальной медицины" (ФГБНУ "ИЭМ" ) RECOMBINANT DNA pA4, RECOMBINANT DNA pQE 30-pA4, PROVIDING OBTAINING POLYPEPTIDE A4, Esherichia coli STRAIN M 15-A4, TRANSFORMED WITH RECOMBINANT PLASMID DNA pQE 30-pA4, AND EXPRESSING RECOMBINANT POLYPEPTIDE A4, RECOMBINANT POLYPEPTIDE A4, POSSESSING ABILITY TO SELECTIVELY BIND HSA, AFFINE SORBENTS (VERSIONS) AND METHODS FOR HSA AND IgG REMOVAL FROM BLOOD SERUM (VERSIONS)
RU2715672C2 (en) * 2017-12-26 2020-03-02 Федеральное государственное бюджетное научное учреждение "Институт экспериментальной медицины" (ФГБНУ "ИЭМ") RECOMBINANT DNA OF pG4223 AND PLASMID DNA PQE 30-pG4223, STRAIN OF ESCHERICHIA COLI M 15-G4223 WHICH ENABLE TO OBTAIN G4223 POLYPEPTIDE SELECTIVELY BINDING IgG, AND USE THEREOF IN AFFINE CHROMATOGRAPHY TO ISOLATE IgG

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