RU93047672A - ENZYME, METHOD FOR ISOLATION OF ENZYME, METHOD FOR ISOLATION OF DIPEPTIDES - Google Patents

ENZYME, METHOD FOR ISOLATION OF ENZYME, METHOD FOR ISOLATION OF DIPEPTIDES

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Publication number
RU93047672A
RU93047672A RU93047672/13A RU93047672A RU93047672A RU 93047672 A RU93047672 A RU 93047672A RU 93047672/13 A RU93047672/13 A RU 93047672/13A RU 93047672 A RU93047672 A RU 93047672A RU 93047672 A RU93047672 A RU 93047672A
Authority
RU
Russia
Prior art keywords
enzyme
dar
isolation
dipeptides
methods
Prior art date
Application number
RU93047672/13A
Other languages
Russian (ru)
Other versions
RU2096455C1 (en
Inventor
Роберт Аткинсон Пол
Дейл Хилтон Мэттью
Карл Ламбуи Питер
Original Assignee
Эли Лилли Энд Компани
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Эли Лилли Энд Компани filed Critical Эли Лилли Энд Компани
Publication of RU93047672A publication Critical patent/RU93047672A/en
Application granted granted Critical
Publication of RU2096455C1 publication Critical patent/RU2096455C1/en

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Claims (1)

Изобретение относится к новой дипептидидаминопептидазе, выделенной из клеточного слизевого грибка Dictyostelium discoideum. Новый фермент ДАП, d ДАР проявляет активность, которая в некоторой степени присуща как АР-I, так и ДАР-III, однако заметно отличается от этих ферментов по своим физическим и другим характеристикам. Изобретение также направлено на разработку методов использования фермента d ДАР для выделения дипептидов у N-концов рекомбинантно полученных исходных белков и пептидов, а также методов выделения и очистки фермента d ДАР из культуры Dictyostelium discoideum.The invention relates to a new dipeptididopeptidase isolated from the cellular mucous fungus Dictyostelium discoideum. The new enzyme DAP, d DAR is active, which is somewhat inherent in both AP-I and DAR-III, but it differs markedly from these enzymes in its physical and other characteristics. The invention is also directed to the development of methods for using the enzyme d DAR to isolate dipeptides from the N-termini of recombinantly obtained starting proteins and peptides, as well as methods for isolating and purifying the enzyme d DAR from a culture of Dictyostelium discoideum.
RU9393047672A 1992-10-01 1993-09-30 Dipeptidylaminopeptidase and method of enzymatic splitting off n-terminal dipeptides met-tyr, met-arg or met-asp from the polypeptide sequence RU2096455C1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95553992A 1992-10-01 1992-10-01
US07/955,539 1992-10-01

Publications (2)

Publication Number Publication Date
RU93047672A true RU93047672A (en) 1996-04-20
RU2096455C1 RU2096455C1 (en) 1997-11-20

Family

ID=25496954

Family Applications (1)

Application Number Title Priority Date Filing Date
RU9393047672A RU2096455C1 (en) 1992-10-01 1993-09-30 Dipeptidylaminopeptidase and method of enzymatic splitting off n-terminal dipeptides met-tyr, met-arg or met-asp from the polypeptide sequence

Country Status (26)

Country Link
US (2) US5565349A (en)
EP (2) EP1094118A1 (en)
JP (1) JP3550409B2 (en)
KR (1) KR100291528B1 (en)
CN (1) CN1060212C (en)
AT (1) ATE211169T1 (en)
AU (1) AU669735B2 (en)
BR (1) BR9303949A (en)
CA (1) CA2107279A1 (en)
CZ (2) CZ286950B6 (en)
DE (1) DE69331371T2 (en)
DK (1) DK0595476T3 (en)
ES (1) ES2169036T3 (en)
FI (1) FI934305A (en)
HU (1) HU219771B (en)
IL (1) IL107123A (en)
MX (1) MX9306021A (en)
MY (1) MY113378A (en)
NO (1) NO933503L (en)
NZ (1) NZ248810A (en)
PL (1) PL176930B1 (en)
PT (1) PT595476E (en)
RU (1) RU2096455C1 (en)
TW (1) TW257792B (en)
UA (1) UA27718C2 (en)
ZA (1) ZA937243B (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5573923A (en) * 1993-12-22 1996-11-12 Eli Lilly And Company Method for removing N-terminal dipeptides from precursor polypeptides with immobilized dipeptidylaminopeptidase from dictyostelium discoideum
US5614379A (en) * 1995-04-26 1997-03-25 Eli Lilly And Company Process for preparing anti-obesity protein
CA2343966A1 (en) * 1998-09-21 2000-03-30 Eli Lilly And Company Production of soluble recombinant trypsinogen analogs
US6743600B1 (en) 1999-04-30 2004-06-01 Monsanto Technologies Llc Method of removing N-terminal alanine residues from polypeptides with Aeromonas aminopeptidase
US6794159B1 (en) 1999-04-30 2004-09-21 Pharmacia Corporation Method of removing n-terminal alanine residues from polypeptides with aeromonas aminopeptidase
US8129139B2 (en) 2009-07-13 2012-03-06 Allergan, Inc. Process for obtaining botulinum neurotoxin
EP2536749A1 (en) * 2010-02-17 2012-12-26 Elona Biotechnologies Methods for preparing human growth hormone
US11427814B2 (en) 2019-03-26 2022-08-30 Encodia, Inc. Modified cleavases, uses thereof and related kits
KR102567902B1 (en) * 2019-03-26 2023-08-18 엔코디아, 인코포레이티드 Modified Cleivases, Their Uses and Related Kits

Family Cites Families (12)

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Publication number Priority date Publication date Assignee Title
DK55685A (en) * 1985-02-07 1986-08-08 Nordisk Gentofte ENZYM OR ENZYM COMPLEX WITH PROTEOLYTIC ACTIVITY
DE3519218A1 (en) * 1985-05-29 1986-12-04 Boehringer Mannheim Gmbh, 6800 Mannheim H (DOWN ARROW) 2 (DOWN ARROW) O (DOWN ARROW) 2 (DOWN ARROW) SARCOSINOXIDASE, ITS PRODUCTION AND USE
US4894439A (en) * 1986-05-22 1990-01-16 Cetus Corporation N-terminal derivatives of tumor necrosis factor purified by microporous PTFE membranes
US5177003A (en) * 1987-04-16 1993-01-05 Phillips Petroleum Company Flavored protein products derived from Candida utilis
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
US5075222A (en) * 1988-05-27 1991-12-24 Synergen, Inc. Interleukin-1 inhibitors
JPH023698A (en) * 1988-06-20 1990-01-09 Denki Kagaku Kogyo Kk Monoclonal antibody against human lymphotoxin, hybridoma capable of producing the same antibody, method for purifying and measuring human lymphotoxin using the same antibody and measuring reagent
US5208218A (en) * 1988-09-19 1993-05-04 Ludwig Institute For Cancer Research T cell growth factor glycoproteins
US5179196A (en) * 1989-05-04 1993-01-12 Sri International Purification of proteins employing ctap-iii fusions
US5175251A (en) * 1989-05-04 1992-12-29 Sri International Antimetastatic peptides with laminin activity
US5126249A (en) * 1989-05-09 1992-06-30 Eli Lilly And Company Enzymatic removal of a protein amino-terminal sequence
US5173409A (en) * 1989-12-08 1992-12-22 Ecogen Inc. Recovery of bt endotoxin protein from lysed cell mixtures

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