RU93042145A - METHOD OF GRADIENT HIGHLY EFFICIENT MEMBRANE CHROMATOGRAPHY (VAMH) OF POLYMERS - Google Patents
METHOD OF GRADIENT HIGHLY EFFICIENT MEMBRANE CHROMATOGRAPHY (VAMH) OF POLYMERSInfo
- Publication number
- RU93042145A RU93042145A RU93042145/25A RU93042145A RU93042145A RU 93042145 A RU93042145 A RU 93042145A RU 93042145/25 A RU93042145/25 A RU 93042145/25A RU 93042145 A RU93042145 A RU 93042145A RU 93042145 A RU93042145 A RU 93042145A
- Authority
- RU
- Russia
- Prior art keywords
- eluent
- composition
- vamh
- peaks
- polymers
- Prior art date
Links
- 238000011140 membrane chromatography Methods 0.000 title 1
- 229920000642 polymer Polymers 0.000 title 1
- 239000000203 mixture Substances 0.000 claims 7
- 239000003480 eluent Substances 0.000 claims 5
- 239000003380 propellant Substances 0.000 claims 2
- 230000037094 Cmin Effects 0.000 claims 1
- 229920001222 biopolymer Polymers 0.000 claims 1
- 238000005194 fractionation Methods 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
Claims (1)
где δφo не превышает разности критических концентраций двух ближайших пиков Δφcmin, а объем подачи элюента данного состава
δvo=δto•F,
где Δto - продолжительность подачи для каждой ступени, F - расход элюента не меньше, чем 4σmax наибольшего значения ширины зоны компонентов, а затем по результирующей хроматограмме задают оптимальное количество ступеней N, равное числу пиков, где высоту каждой ступени δφi определяют по формуле:
δφi=(Ki+1)δφo
где Кi - число пустых интервалов δφo между i и i + 1 пиками, а объем подачи элюента данного состава φi определяют по формуле:
δvi=4δi The proposed solution relates to methods VAMH and will be widely used for the fractionation of complex mixtures of biopolymers, especially on a preparative scale, in the pharmaceutical and medical industry, bioorganic chemistry, biotechnology. The invention allows the separation of complex mixtures of unknown composition to almost the baseline and the optimal way to select fractions in the preparative selection, i.e. to increase the effectiveness of VAMH and the purity of the preparations obtained. This is achieved due to the fact that in the well-known method, based on programming the composition of the eluent by feeding a predetermined concentration to the cell of the propellant solution, a multistep mode of changing the composition of the eluent is used, at which the whole working interval of the propellant Δφ is divided into n equal steps
where δφ o does not exceed the difference of critical concentrations of the two nearest peaks Δφ cmin , and the volume of the eluent feed of a given composition
δv o = δt o • F,
where Δt o is the duration of supply for each stage, F is the eluent flow rate not less than 4σ max of the largest width of the component zone, and then using the resulting chromatogram set the optimal number of steps N equal to the number of peaks, where the height of each step δφ i is determined by the formula :
δφ i = (K i + 1 ) δφ o
where K i - the number of empty intervals δφ o between i and i + 1 peaks, and the amount of eluent flow of a given composition φ i is determined by the formula:
δv i = 4δ i
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU93042145A true RU93042145A (en) | 1996-02-20 |
Family
ID=
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4274967A (en) | Chromatographic apparatus and method | |
| Foley | Optimization of micellar electrokinetic chromatography | |
| Mazzeo et al. | Novel chiral surfactant for the separation of enantiomers by micellar electrokinetic capillary chromatography | |
| US4204952A (en) | Chromatographic apparatus and method | |
| Row et al. | Separation of modified nucleic acid constituents by micellar electrokinetic capillary chromatography | |
| Strausbauch et al. | Sensitivity enhancement and second‐dimensional information from solid phase extraction‐capillary electrophoresis of entire high‐performance liquid chromatography fractions | |
| ATE107534T1 (en) | SEPARATION OF CHARGED MOLECULES. | |
| JP2003156482A (en) | Preparative chromatograph and separation and refining method using it | |
| Hodges et al. | Preparative purification of peptides by reversed-phase chromatography: Sample displacement mode versus gradient elution mode | |
| FR2552231B1 (en) | PROCESS FOR CONCENTRATING THE SOLUTE DURING INJECTION INTO A GAS PHASE CHROMATOGRAPHY COLUMN | |
| Euerby et al. | Step-gradient capillary electrochromatography | |
| US5004547A (en) | Method and apparatus for bioaffinity separation | |
| Sun et al. | Enhanced chiral separation of dansylated amino acids with cyclodextrin‐dextran polymer network by capillary electrophoresis | |
| RU93042145A (en) | METHOD OF GRADIENT HIGHLY EFFICIENT MEMBRANE CHROMATOGRAPHY (VAMH) OF POLYMERS | |
| Hodges et al. | Multi-column preparative reversed-phase sample displacement chromatography of peptides | |
| EP1044716A1 (en) | Micropreparative isoelectric focussing | |
| Tehrani et al. | Capillary electrophoresis: An integrated system with a unique split‐flow sample introduction mechanism | |
| Sun et al. | Comparison of micellar electrokinetic capillary chromatography and high-performance liquid chromatography on the separation and determination of caffeine and its analogues in pharmaceutical tablets | |
| Burke et al. | A novel approach to reversed-phase preparative high-performance liquid chromatography of peptides | |
| Rathore | Resin screening to optimize chromatographic separations | |
| Fiedler et al. | Separation of amino acids and antibiotics by narrow-bore and normal-bore high-performance liquid chromatography with pre-column derivatization | |
| Caslavska et al. | Purification of ovalbumin and lysozyme from a commercial product by recycling isotachophoresis | |
| Jorgenson | Trends in analytical scale separations | |
| Burke et al. | Preparative Reversed-Phase Shallow Gradient Approach to the Purification of Closely-Related Peptide Analogs on Analytical Instrumentation | |
| Raymond et al. | Separation and Recovery of Proteins by Elution-Convection |