RU93033302A - CULTURED CELLS OF PLANTS OF ERITRICHIUM INCANUM (TURCZ.) A.DC.SSP.SICHOTENSE (M.POP) STARCHENKO, USED TO RECEIVE A DRUG, PRODUCTIVATING CHITIN THIN CHATOTH CHRISTOX CHROSTIAL TOSTS, - Google Patents

CULTURED CELLS OF PLANTS OF ERITRICHIUM INCANUM (TURCZ.) A.DC.SSP.SICHOTENSE (M.POP) STARCHENKO, USED TO RECEIVE A DRUG, PRODUCTIVATING CHITIN THIN CHATOTH CHRISTOX CHROSTIAL TOSTS,

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Publication number
RU93033302A
RU93033302A RU93033302/13A RU93033302A RU93033302A RU 93033302 A RU93033302 A RU 93033302A RU 93033302/13 A RU93033302/13 A RU 93033302/13A RU 93033302 A RU93033302 A RU 93033302A RU 93033302 A RU93033302 A RU 93033302A
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RU
Russia
Prior art keywords
drug
activity
chitinase
sichotense
starchenko
Prior art date
Application number
RU93033302/13A
Other languages
Russian (ru)
Other versions
RU2044052C1 (en
Inventor
Т.И. Музарок
Ю.Н. Журавлев
Л.И. Федореева
В.И. Горбач
Л.Д. Селецкая
И.Т. Ожигова
Л.С. Лауве
Original Assignee
Биолого-почвенный институт Дальневосточного отделения РАН
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Publication date
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Priority to RU93033302A priority Critical patent/RU2044052C1/en
Priority claimed from RU93033302A external-priority patent/RU2044052C1/en
Application granted granted Critical
Publication of RU2044052C1 publication Critical patent/RU2044052C1/en
Publication of RU93033302A publication Critical patent/RU93033302A/en

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Claims (1)

Штамм культивируемых клеток растений незабудочника сихотинского Eritrichium incanum (Turcz.) A. DC. ssp. sichotense (M. Pop) Starchenko, используемый для получения препарата, обладающего хитиназной и хитозаназной активностью, относится к биотехнологии. Штамм ErSR получен из дикорастущего растения незабудочника сихотинского, заготовленного в Партизанском районе Приморского края в фазе цветения. Ростовой индекс культуры 10±3. Штамм культивируемых клеток растений ErSR является новым источником для биотехнологического получения препарата, обладающего хитиназной и хитозаназной активностью. Препарат представляет собой белковую фракцию, выделенную из биомассы клеток штамма ErSR, и имеет следующие характеристики (из расчета на 1 г сырых клеток): концентрация белка - 2,05 мг; суммарная хитиназная активность - 4210 мкМ; суммарная хитозаназная активность - 585 мкМ; удельная хитиназная активность - 2050 мкМ/мг; удельная хитозаназная активность - 285 мкМ/мг. Штамм культивируемых клеток растений незабудочника сихотинского ErSR является высокопродуктивным и возобновимым источником биотехнологического получения препарата, обладающего хитиназной и хитозаназной активностью.Strain of cultured plant cells of the non-mate Sichotina Eritrichium incanum (Turcz.) A. DC. ssp. sichotense (M. Pop) Starchenko, used to obtain a drug with chitinase and chitosanase activity, refers to biotechnology. The ErSR strain was obtained from a wild-growing plant of the non-forgetting sikhotinsky, harvested in the Partizansky district of the Primorsky Territory in the flowering phase. Growth index of culture 10 ± 3. The strain of cultured plant cells ErSR is a new source for biotechnological production of the drug, which has chitinase and chitosanase activity. The drug is a protein fraction isolated from the biomass of ErSR cells, and has the following characteristics (based on 1 g of raw cells): protein concentration - 2.05 mg; total chitinase activity - 4210 μM; total chitosanase activity - 585 μM; specific chitinase activity - 2050 μM / mg; specific chitosanase activity - 285 μM / mg. The strain of cultivated plant cells of the non-forgetful of the sihotinskiy ErSR is a highly productive and renewable source of biotechnological production of the drug, which has chitinase and chitosanase activity.
RU93033302A 1993-06-25 1993-06-25 Strain of cultured plant cells eritrichium incanum (turcz) (a.dc.ssp.) sichotense (m.pop.) starchenko used for preparing the preparation showing chitinase and chirosanase activity RU2044052C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU93033302A RU2044052C1 (en) 1993-06-25 1993-06-25 Strain of cultured plant cells eritrichium incanum (turcz) (a.dc.ssp.) sichotense (m.pop.) starchenko used for preparing the preparation showing chitinase and chirosanase activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU93033302A RU2044052C1 (en) 1993-06-25 1993-06-25 Strain of cultured plant cells eritrichium incanum (turcz) (a.dc.ssp.) sichotense (m.pop.) starchenko used for preparing the preparation showing chitinase and chirosanase activity

Publications (2)

Publication Number Publication Date
RU2044052C1 RU2044052C1 (en) 1995-09-20
RU93033302A true RU93033302A (en) 1996-03-10

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RU93033302A RU2044052C1 (en) 1993-06-25 1993-06-25 Strain of cultured plant cells eritrichium incanum (turcz) (a.dc.ssp.) sichotense (m.pop.) starchenko used for preparing the preparation showing chitinase and chirosanase activity

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