RU93017669A - METHOD FOR ISOLATION OF MONONUCLERIERS FROM HUMAN BLOOD - Google Patents

METHOD FOR ISOLATION OF MONONUCLERIERS FROM HUMAN BLOOD

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Publication number
RU93017669A
RU93017669A RU93017669/14A RU93017669A RU93017669A RU 93017669 A RU93017669 A RU 93017669A RU 93017669/14 A RU93017669/14 A RU 93017669/14A RU 93017669 A RU93017669 A RU 93017669A RU 93017669 A RU93017669 A RU 93017669A
Authority
RU
Russia
Prior art keywords
human blood
mononucleriers
isolation
mononuclear cells
centrifugation
Prior art date
Application number
RU93017669/14A
Other languages
Russian (ru)
Other versions
RU2061958C1 (en
Inventor
А.Т. Мамасаидов
Е.В. Бененсон
М.В. Коростелева
Г.С. Приказчикова
Original Assignee
Кировский филиал Пермского государственного медицинского института
Filing date
Publication date
Application filed by Кировский филиал Пермского государственного медицинского института filed Critical Кировский филиал Пермского государственного медицинского института
Priority to RU93017669A priority Critical patent/RU2061958C1/en
Priority claimed from RU93017669A external-priority patent/RU2061958C1/en
Publication of RU93017669A publication Critical patent/RU93017669A/en
Application granted granted Critical
Publication of RU2061958C1 publication Critical patent/RU2061958C1/en

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Claims (1)

Способ выделения мононуклеаров из крови человека заключается в следующем: на 3 мл смеси (1 ч 76%-ного верографина и 4 ч 3,75%-ного поливинилпиролидона) наслаивают 6 мл цельной стабилизированной антикоагулянтом крови, затем проводят центрифугирование при 400 д в течение 30 мин. После центрифугирования отсасывают пастеровской пипеткой слой мононуклеаров (плотное облачко над смесью). Выделенные клетки отмывают 1 раз не менее, чем четырехкратным избытком питательной среды, затем мононуклеары концентрируют центрифугированием при 400 g в течение 10 мин.The method for isolating mononuclear cells from human blood is as follows: 6 ml of whole, anticoagulant-stabilized blood is layered on 3 ml of the mixture (1 h of 76% vegragraphin and 4 h of 3.75% polyvinylpyrrolidone), then centrifuged at 400 d for 30 min After centrifugation, a layer of mononuclear cells is sucked off with a Pasteur pipette (a dense cloud above the mixture). The isolated cells are washed once with not less than fourfold excess of the nutrient medium, then the mononuclear cells are concentrated by centrifugation at 400 g for 10 minutes.
RU93017669A 1993-04-05 1993-04-05 Method for separating mononuclears from the human blood RU2061958C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU93017669A RU2061958C1 (en) 1993-04-05 1993-04-05 Method for separating mononuclears from the human blood

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU93017669A RU2061958C1 (en) 1993-04-05 1993-04-05 Method for separating mononuclears from the human blood

Publications (2)

Publication Number Publication Date
RU93017669A true RU93017669A (en) 1995-12-10
RU2061958C1 RU2061958C1 (en) 1996-06-10

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU93017669A RU2061958C1 (en) 1993-04-05 1993-04-05 Method for separating mononuclears from the human blood

Country Status (1)

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RU (1) RU2061958C1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2542450C1 (en) * 2013-07-25 2015-02-20 федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Ростовский государственный строительный университет" РГСУ Method for determining blood cell adhesiveness
RU2727007C1 (en) * 2019-04-10 2020-07-17 Федеральное государственное бюджетное образовательное учреждение высшего образования "Саратовский государственный аграрный университет имени Н.И. Вавилова" Method for production of bovine lymphocytes

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