RU92003601A - NUTRIENT ENVIRONMENT FOR THE ACCELERATED ISOLATION AND DETERMINATION OF ANTIBIOTIC SENSITIVITY OF GRADUATIVE MICROORANISMS - Google Patents

Claims (1)

The invention relates to the field of medicine, mainly to clinical and sanitary microbiology, and relates to nutrient media for the accelerated isolation and determination of an antibiogram of gram-negative microorganisms. The aim of the study is to obtain a cumulative environment suitable for the accelerated cultivation and determination of antibiotic sensitivity of gram-negative bacteria. As the main source of nitrogen nutrition in the proposed medium, a new preparation of amino acids, isolated from Baker's yeast, was used. The preparation contains 18 amino acids necessary for the growth of microorganisms. Experimental data indicate that the growth characteristics of Escherichia coli on a new nutrient medium, such as the maximum growth rate, the minimum generation time, the lag phase duration, are similar to or exceed the corresponding characteristics for the prototype environment (the Abbot medium for Gram-). At the same time, the developed medium provides the maximum accumulation of culture in 2.0 - 2.5 times greater than in the prototype environment by 4 - 6 hours from the time of sowing. The effectiveness of the new environment for other representatives of gram-negative bacteria was shown. The developed environment is elective, highly sensitive and provides rapid accumulation and determination of the antibiogram of gram-negative bacteria. The medium can be used both in automated microbiological centers and in routine research methods.