RU92001266A - METHOD OF OBTAINING NON-RADIOACTIVE-COMBINED PROBE FOR DETERMINING NUCLEOTIDE SEQUENCES - Google Patents

METHOD OF OBTAINING NON-RADIOACTIVE-COMBINED PROBE FOR DETERMINING NUCLEOTIDE SEQUENCES

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Publication number
RU92001266A
RU92001266A RU92001266/13A RU92001266A RU92001266A RU 92001266 A RU92001266 A RU 92001266A RU 92001266/13 A RU92001266/13 A RU 92001266/13A RU 92001266 A RU92001266 A RU 92001266A RU 92001266 A RU92001266 A RU 92001266A
Authority
RU
Russia
Prior art keywords
radioactive
nucleotide sequences
obtaining non
combined probe
reacts
Prior art date
Application number
RU92001266/13A
Other languages
Russian (ru)
Other versions
RU2049821C1 (en
Inventor
В.А. Адаричев
Г.М. Дымшиц
С.М. Калачиков
Original Assignee
Институт цитологии и генетики СО РАН
Filing date
Publication date
Application filed by Институт цитологии и генетики СО РАН filed Critical Институт цитологии и генетики СО РАН
Priority to RU92001266A priority Critical patent/RU2049821C1/en
Priority claimed from RU92001266A external-priority patent/RU2049821C1/en
Application granted granted Critical
Publication of RU2049821C1 publication Critical patent/RU2049821C1/en
Publication of RU92001266A publication Critical patent/RU92001266A/en

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Claims (1)

Использование: биотехнология, медицина, сельское хозяйство. Задача изобретения: повышение качества целевого продукта. Сущность изобретения: в качестве переаминирующего реагента используют соединения общей формулы R1-S-R2, где R1 - производное гидроксиламина, реагирующее с цитозином в составе нуклеиновой кислоты; S - спейсер; R2 - химическая группировка, реагирующая с активированными производными сигнальных соединений (аминогруппа, аминооксигруппа), а процесс переаминирования проводят при pH 3,2 - 4,5 при 60 - 90oС в течение 1 - 12 мин. Технический результат: увеличение чувствительности при выявлении зонда, расширение круга модифицируемых нуклеиновых кислот (одно- и двуцепочечные поли- и олигонуклеотиды, дезокси- и рибопроизводные), а также увеличение стабильности получающегося продукта.Uses: biotechnology, medicine, agriculture. The objective of the invention: improving the quality of the target product. The essence of the invention: as a transaminating reagent using compounds of the general formula R 1 -SR 2 , where R 1 is a hydroxylamine derivative, which reacts with cytosine in the composition of a nucleic acid; S - spacer; R 2 is a chemical group that reacts with activated derivatives of signal compounds (amino group, aminooxy group), and the transamination process is carried out at pH 3.2 - 4.5 at 60 - 90 o С for 1 - 12 minutes. EFFECT: increased sensitivity in detecting a probe, expanding the range of modifiable nucleic acids (single and double stranded poly- and oligonucleotides, deoxy- and rib-derivatives), as well as increasing the stability of the resulting product.
RU92001266A 1992-10-20 1992-10-20 Method of preparing non-radioactive labelled probe for determination of nucleotide series RU2049821C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU92001266A RU2049821C1 (en) 1992-10-20 1992-10-20 Method of preparing non-radioactive labelled probe for determination of nucleotide series

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU92001266A RU2049821C1 (en) 1992-10-20 1992-10-20 Method of preparing non-radioactive labelled probe for determination of nucleotide series

Publications (2)

Publication Number Publication Date
RU2049821C1 RU2049821C1 (en) 1995-12-10
RU92001266A true RU92001266A (en) 1996-04-10

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU92001266A RU2049821C1 (en) 1992-10-20 1992-10-20 Method of preparing non-radioactive labelled probe for determination of nucleotide series

Country Status (1)

Country Link
RU (1) RU2049821C1 (en)

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