RU86101U1 - NANOCONTAINER WITH AN END PLUG AND A PERFORATED END PLUG - Google Patents

Abstract

A nanocontainer with an end cap and a perforated end cap, characterized in that it is made in the form of a roll, the surface of the outer part of which is formed of SiO2, and the channel surface is Al2O3, while the distance between the layers forming the roll is in the range from 0.18 ∙ 10 -9 to 2.5 ∙ 10-9 m, the roll length ranges from 6 ∙ 10-8 to 9 ∙ 10-6 m, the channel diameter is in the range from 5 ∙ 10-9 to 2.1 ∙ 10-7 m, and the number of layers of the roll is chosen equal to one of the values of the range from 2 to 50, while the channel of the said roll wb izi first end provided with a solid plug, and near the second end provided with a perforated stopper.

Description

The utility model relates to the field of nanotechnology and can be used to place microscopic doses of various materials in the channel of a roll of a nanocontainer equipped with an end cap and a perforated end cap, with the possibility of subsequent release of the contained material from the device into its external environment.

The prior art nanocontainer [1], which is used to place certain compositions in it. These formulations are intended for skin care and are both ordinary vitamins and vitamin complexes in the form of appropriate aqueous solutions. The nanocontainer under consideration is made of tubular halloysite (halloysite nanotube) with a length of 1 × 10 ^-7 to 4 × 10 ^-5 meters, with an outer diameter (surface of which consists of Si0 ₂ ) of the tube (tube) forming the nanocontainer roll, in the range of 1 × 10 ^-8 to 5 × 10 ^-7 meters. Moreover, the diameter of the channel of the said roll (the surface of which is already Al ₂ O ₃ ) does not exceed 2 × 10 ^-7 meters and, basically, is about 4 × 10 ^-7 meters.

The disadvantage of the analog device is the small (not exceeding 16 hours) release time of the above composition from the channel of the roll into the surrounding external analog device.

The closest in technical essence and the achieved result is a nanocontainer [2], which is made in the form of a tubular multilayer roll (tubular multilayer shell) so that the distance between the layers lies in the range from 0.18 × 10 ^-9 to 2.5 × 10 ^{- 9} meters, and the surface of the said roll is formed by Si0 ₂ , and the surface of its channel is from Al ₂ O ₃ . The length of the prototype nanocontainer lies in the range from 6 × 10 ^-8 to 9 × 10 ^-6 meters, and the channel diameter (the diameter of the inner part of the tubular multilayer shell) corresponds to a range of values from 5 × 10 ^-9 to 2.1 × 10 ^-7 meters . The prototype nanocontainer under consideration is also characterized by a certain number of layers ranging from 2 to 50. The disadvantage of the prototype nanocontainer is the relatively short (not exceeding 194 hours) time of release of the material contained in it into the environment.

The task to which the creation of this device is directed is the development of a promising means for containing microscopic doses of materials, ensuring its subsequent prolonged release into the environment.

The technical result expected from the use of the claimed device consists in prolonging the time of release of the material contained therein into the external environment.

The claimed technical result is achieved in that the nanocontainer with an end cap and a perforated end cap is made in the form of a roll, the surface of the outer part of which is formed of Si0 ₂ , and the channel surface is Al ₂ O ₃ , while the distance between the layers forming the roll is in the range from 0.18 × 10 ^-9 to 2.5 × 10 ^-9 meters, the length of the roll is in the range from 6 × 10 ^-8 to 9 × 10 ^-6 meters, the diameter of the channel is in the range from 5 × 10 ^-9 to 2, 1 × 10 ^-7 meters, and the number of coil layers is selected to be one of a range of values of 2-50, n and said channel of said coil near the first end is provided with a solid plug, and near the second end provided with a perforated stopper.

The utility model is illustrated by drawings. Figure 1 conditionally presents a longitudinal section of a nanocontainer before placing continuous and perforated plugs in its channel; figure 2 conditionally presents a longitudinal section of a nanocontainer with a continuous plug placed near the first end; figure 3 conditionally shows a longitudinal section of a nanocontainer with a continuous stopper located near the first end and a perforated stopper located near the second end; figure 4 schematically shows the appearance of the roll from the side of the first end; figure 5 schematically shows the appearance of the roll from the side of the second end.

The list of positions.

1. Roll (tubular multilayer shell).

11. The channel of the roll.

12. The diameter of the channel of the roll.

13. The length of the roll.

14. The distance between the layers of the roll.

2. A continuous cork.

3. Perforated cork.

The rolls of the claimed device 1 (FIG. 1 to FIG. 5), as well as the prototype nanocontainer, were made from mineral clay materials (halloysite) by initially coarsely crushing gallausite into pieces, further grinding these pieces into fine grinding and then washing the rolls 1 (FIG. .1) in the flow of a liquid, for example, water ([3] and [4]).

Using methods and techniques known from [5], weighed portions were prepared, then sorted by geometrical parameters.

As the mentioned parameters were used, the diameter (from 5 × 10 ^-9 to 2.1 × 10 ^-7 meters) of the channel of the roll 12 (Figure 1), the length (from 6 × 10 ^-8 to 9 × 10 ^-6 meters) of the roll 13 (Fig. 3), the distance (from 0.18 × 10 ^-9 to 2.5 × 10 ^-9 meters) between the layers of roll 14 (Fig. 1), as well as the number of layers (the value of this parameter is in the range from 2 up to 50 layers) in a roll 1 (Figure 1).

Example 1

For the first example, 5 × 10 ⁻³ Kg of rolls 1 (FIG. 1) were used, and the length of roll 13 (FIG. 1) was 5 × 10 ^-7 meters, the distance between the layers of roll 12 (FIG. 1) was 0.18 × 10 ^-9 meters, and the diameter of the channel of the roll was 5 × 10 ^-9 meters. The shell of the rolls 1 (Fig.1-Fig.5) is formed by 25 layers.

As containerized material to fill the channels of the roll 11 (Figure 1) used benzotriazole (functionally characterized as an anti-corrosion inhibitor).

Initially, 20 ml of water with a pH7 heated to a temperature of 293.15 K at atmospheric pressure of 101300 Pa and a relative humidity of 70% was placed in an ultrasonic bath. 5 × 10 ^-3 Kg of rolls 1 (Figure 1) and 3.2 × 10 ^-3 Kg of fullerenes with dimensions of about 4.5 × 10 ^-9 meters were successively poured there. Then, the source of ultrasonic vibrations with which the bath was equipped was turned on, and the resonance frequency of the mixture was determined (the search was performed in the frequency range from 20 to 50 KHz) according to the technique disclosed in [6].

In accordance with the recommendations set forth in the previously mentioned source of information [6], the shape of the ultrasonic signal was either rectangular, sinusoidal, or sawtooth. After 21 minutes of ultrasonic exposure at the detected resonant frequency to the mixture in the ultrasonic bath (21 minutes is the experimentally established time of guaranteed blocking of the end face of roll 1 by fullerene (Figure 2) under the indicated conditions), the processing was stopped.

The contents of the ultrasonic bath were removed and rinsed for 45 seconds in a stream of water with a pH of 6.8 (the flow rate of which was 1.7 l / min, and the temperature was 293.15 K). After that, the extracted rolls 1 (Figure 2), one of the ends of which was equipped with a continuous cork 2 (Figure 2) from fullerene, was subjected to vacuum drying at a pressure of 550 Pa for 10 minutes.

Then, 22 ml of a 5% solution of benzotriazole in acetone were placed in an ultrasonic bath at a temperature of 288 K, atmospheric pressure of 101325 Pa and relative humidity of 70%. Vacuum dried rolls 1 were added to a solution of benzotriazole in acetone (Figure 2). After that, 3.1 × 10 ^-3 Kg of short carbon nanotubes [7] with a diameter not exceeding 4.8 × 10 ^-9 meters were poured into the mixture.

Again, the source of ultrasonic vibrations with which the bath was equipped was turned on, and the resonant frequency of the resulting mixture was determined (the search was performed in the frequency range from 20 to 50 KHz) according to the technique disclosed in [6].

In accordance with the recommendations set forth in the source of information [6], the shape of the ultrasonic signal was set to either rectangular, sinusoidal, or sawtooth. After 14 minutes of ultrasonic exposure at the detected resonant frequency to the mixture in the ultrasonic bath (14 minutes is the experimentally established time of guaranteed blockage by a short carbon nanotube 3 (Figure 3) of the second end of roll 1 (Figure 3) under the selected conditions), the ultrasonic treatment was completed .

The contents of the ultrasonic bath were removed and rinsed for 2 minutes in a stream of water with pH7 (the flow rate of which was 3.3 l / min, and the temperature was 293.15 K). After that, the extracted rolls 1 (Figure 3) were mixed in 15 ml of water with a pH of 6.8 and a temperature of 303 K, where the benzotriazole was gradually released from nanocontainers with an end cap and a perforated end cap in an external aqueous medium.

The dynamics of the increase in the concentration of benzotriazole in the external aquatic environment was monitored by UV photometry using an Agilent-8453 device (UV spectrophotometer) with an accuracy of 1%.

The results of a comparison with the prototype device measuring the dynamics of the release of benzotriazole from the proposed device into the external environment are shown in Table 1.

Table 1 The amount of benzotriazole released into the water, (%) 10 twenty thirty 40 fifty 60 70 80 90 one hundred Benzotriazole release time (hours) from prototype device 0.8 1,5 3.0 7.3 14.7 27.4 44.7 68,2 89.5 - Benzotriazole release time (hours) from the proposed nanocontainer 32 79 131 188 240 295 353 406 447 -

As follows from Table 1, the proposed device provides (relative to the prototype device) a significant increase in the time of allocation of benzotriazole.

Example 2

For the second example, 5.8 × 10 ⁻³ Kg of rolls 1 (FIG. 1) were used, and the length of roll 13 (FIG. 1) was 6 × 10 ^-8 meters, the distance between the layers of roll 12 (FIG. 1) was 2 , 5 × 10 ^-9 meters, and the diameter of the channel of the roll was 3 × 10 ^-8 meters. The shell of the rolls 1 (Fig.1-Fig.5) was formed by 2 layers.

As the material for filling the channel of the roll 11 (Figure 1), insulin (a medicine for stopping the manifestations of the disease "diabetes") was used.

Initially, 18 ml of water (pH6.6), heated to a temperature of 298 K at atmospheric pressure of 101400 Pa and a relative humidity of 68%, was placed in an ultrasonic bath. 5.8 × 10 ^-3 Kg of rolls 1 (Figure 1) and 3.0 × 10 ^-3 Kg of fullerenes with dimensions of about 2.7 × 10 ^-8 meters were successively poured there. Then, the source of ultrasonic vibrations with which the bath was equipped was turned on, and the resonance frequency of the mixture was determined (the search was performed in the frequency range from 20 to 50 KHz) according to the technique disclosed in [6].

In accordance with the recommendations set forth in the above-mentioned source of information [6], the shape of the ultrasonic signal was either rectangular, sinusoidal, or sawtooth. After 20 minutes of ultrasonic exposure at the detected resonant frequency to the mixture in the ultrasonic bath (20 minutes is the experimentally established time of guaranteed blocking of the butt end of roll 1 by fullerene (Figure 2) under the selected conditions), the treatment was stopped.

The contents of the ultrasonic bath were removed and rinsed for 40 seconds in a stream of water with pH7 (the flow rate of which was 2.2 l / min and the temperature was 298 K). After that, the extracted rolls 1 (Figure 2), one of the ends of which was equipped with a continuous cork 2 (Figure 2) from fullerene, was subjected to vacuum drying at a pressure of 600 Pa for 12 minutes.

Then 25 ml of a 3% solution of insulin in water (pH6.7) was placed in an ultrasonic bath at a temperature of 288 K, atmospheric pressure of 101400 Pa and relative humidity of 68%. Into the indicated insulin solution, rolls 1 dried by vacuum were added (FIG. 2). After that, 2.8 × 10 ^-3 Kg of short carbon nanotubes [7] with a diameter not exceeding the value of 2.9 × 10 ^-8 meters were poured into the mixture.

Again, the source of ultrasonic vibrations with which the bath was equipped was turned on, and the resonant frequency of the resulting mixture was determined (the search was performed in the frequency range from 20 to 50 KHz) according to the technique disclosed in [6].

In accordance with the recommendations set forth in the source of information [6], the shape of the ultrasonic signal was set to either rectangular, sinusoidal, or sawtooth. After 15 minutes of ultrasonic exposure at the detected resonant frequency to the mixture in the ultrasonic bath (15 minutes is the experimentally established time of guaranteed blockage by a short carbon nanotube 3 (Figure 3) of the second end of roll 1 (Figure 3) under the selected conditions), the ultrasonic treatment was completed .

The contents of the ultrasonic bath were removed and rinsed for 2.5 minutes in a stream of water with a pH of 6.8 (the flow rate of which was 3.0 l / min, and the temperature was 298 K). After that, the extracted rolls 1 (Fig. 3) were mixed in 18 ml of water with pH7 and a temperature of 298 K, where insulin was gradually released from nanocontainers with an end cap and a perforated end cap in an external aqueous medium.

The dynamics of the increase in insulin concentration in the external (relative to the proposed nanocontainer) aqueous medium was controlled by UV photometry using an Agilent-8453 device (UV spectrophotometer) with an accuracy of 1%.

The results of the comparison with the prototype device, measuring the dynamics of the release of insulin from the proposed device into the environment are shown in Table 2.

table 2 The amount of insulin released into the water, (%) 10 twenty thirty 40 55 65 75 85 95 one hundred Insulin release time (hours) from the prototype device fourteen 25 46 70 112 143 164 176 181 - The time of isolation of insulin (hours) from the proposed nanocontainer 81 175 211 278 340 412 490 562 616 -

As follows from Table 2, the proposed device provides (relative to the prototype device) a significant increase in the time of isolation of insulin.

Example 3

For the third example, 4.6 × 10 ⁻³ Kg of rolls 1 (FIG. 1) were used, and the length of roll 13 (FIG. 1) was 9 × 10 ^-6 meters, the distance between the layers of roll 12 (FIG. 1) was 1 , 1 × 10 ^-9 meters, and the diameter of the channel of the roll was 2.1 × 10 ^-7 meters. The shell of the rolls 1 (Fig.1-Fig.5) is formed of 50 layers.

As containerized material to fill the channel of the roll 11 (Figure 1) was used catalase (related to protein enzymes).

Initially, 24 ml of water (pH6.8), heated to a temperature of 283 K at atmospheric pressure of 98,050 Pa and a relative humidity of 50%, was placed in an ultrasonic bath. There, 4.6 × 10 ^-3 Kg of rolls 1 (Figure 1) and 2.5 × 10 ^-3 Kg of fullerenes with dimensions of about 2 × 10 ^-7 meters were successively poured there. Then, the source of ultrasonic vibrations with which the bath was equipped was turned on, and the resonance frequency of the mixture was determined (the search was performed in the frequency range from 20 to 50 KHz) according to the technique disclosed in [6].

In accordance with the recommendations set forth in the above-mentioned source of information [6], the shape of the ultrasonic signal was either rectangular, sinusoidal, or sawtooth. After 23 minutes of ultrasonic exposure at the detected resonant frequency to the mixture in the ultrasonic bath (23 minutes is the experimentally established time of guaranteed blocking of the butt end of roll 1 by fullerene (Figure 2) under the selected conditions), the treatment was stopped.

The contents of the ultrasonic bath were removed and rinsed for 1 minute in a stream of water with a pH of 6.8 (the flow rate of which was 1.4 l / min, and the temperature was 283 K). After that, the extracted rolls 1 (Fig. 2), one of the ends of which was equipped with a continuous cork 2 (Fig. 2) made of fullerene, was vacuum dried at a pressure of 110 Pa for 8 minutes.

Then 26 ml of catalase was placed in an ultrasonic bath at a temperature of 283 K, atmospheric pressure 101320 Pa and a relative humidity of 50%. Vacuum dried rolls 1 were added to catalase (Figure 2). After that, 3.5 × 10 ^-3 Kg of short carbon nanotubes [7] with a diameter not exceeding 2 × 10 ^-7 meters were poured into the mixture.

Again, the source of ultrasonic vibrations with which the bath was equipped was turned on, and the resonant frequency of the resulting mixture was determined (the search was performed in the frequency range from 20 to 50 KHz) according to the technique disclosed in [6].

In accordance with the recommendations set forth in the source of information [6], the shape of the ultrasonic signal was set to either rectangular, sinusoidal, or sawtooth. After 17 minutes of ultrasonic exposure at the detected resonant frequency to the mixture in the ultrasonic bath (17 minutes is the experimentally established time of guaranteed blockage by a short carbon nanotube 3 (Figure 3) of the second end of roll 1 (Figure 3) under the selected conditions), the ultrasonic treatment was stopped .

The contents of the ultrasonic bath were removed and rinsed for 4.5 minutes in a stream of water with pH7 (the flow rate of which was 2.0 l / min, and the temperature was 293 K). After that, the extracted rolls 1 (Figure 3) were mixed in 21 ml of water with a pH of 6.8 and a temperature of 293 K, where the catalase was released from nanocontainers with an end cap and a perforated end cap into the external environment.

The dynamics of the increase in the concentration of catalase in the external (relative to the proposed nanocontainer) aqueous medium was monitored using UV photometry using an Agilent-8453 device (UV spectrophotometer) with an accuracy of 1%.

The results of a comparison with a prototype device measuring changes in the dynamics of catalase release from the proposed device into the environment are shown in Table 3.

Table 3 The amount of catalase released into the water, (%) 10 twenty thirty 40 fifty 60 70 80 90 one hundred Catalase isolation time (hours) from the prototype device fifteen 27 39 fifty 67 85 96 122 132 - Catalase isolation time (hours) from the proposed anocontainer 81 165 221 298 355 431 496 564 616 -

As follows from Table 3, the proposed device provides (relative to the prototype device) a significant increase in the time of allocation of catalase.

The data obtained, presented in Tables 1-3, give reason to confirm the achievement of the proposed device the claimed technical result.

Information sources

1. Application for US invention No. 2007/0202061, IPC: A61K 8/49, "Cosmetic skincare applications employing mineral-derived tubules for controlled release", publ. 08/30/2007

2. Utility model of the Russian Federation No. 71543, IPC: A61K 8/28, Nanocontainer, publ. March 20, 2008 (prototype)

3. Clay Minerals Magazine, v.40, p. 383-426, article Halloysite Clay Minerals, E. Joussein and all, 2005.

4. The journal Small (Nano, Micro), v.1, p. 510-513, article “Biomimetic Synthesis of Vaterite in the Interior of Clay Nanotubules”, D. Shchukin and all., 2005.

5. US invention No. 7425232, IPC: F17C 11/00, "Hydrogen storage apparatus comprised of halloysite", publ. September 16, 2008

6. The invention of the Russian Federation No. 2283273, IPC: C1B 31/02, "A method of obtaining a solution of fullerenes", publ. September 10, 2006

7. The invention of the Russian Federation No. 23039118, IPC: C01B 31/02, "Short carbon nanotubes", publ. 10/27/2007

Claims (1)

A nanocontainer with an end cap and a perforated end cap, characterized in that it is made in the form of a roll, the surface of the outer part of which is formed of SiO ₂ and the channel surface is Al ₂ O ₃ , while the distance between the layers forming the roll is in the range from 0 , 18 ∙ 10 ^-9 to 2.5 ∙ 10 ^-9 m, the length of the roll is in the range from 6 ∙ 10 ^-8 to 9 ∙ 10 ^-6 m, the channel diameter is in the range from 5 ∙ 10 ^-9 to 2.1 ∙ 10 ^-7 m, and the number of layers of the roll is chosen equal to one of the values of the range from 2 to 50, while the channel of the said roll it is equipped with a continuous plug near the first end, and with a perforated plug near the second end.