RU81199U1 - PERFUSION CAMERA FOR SURGEON DRUG - Google Patents

Abstract

The perfusion chamber device for a surviving microscopic preparation contains a tube for supplying saline solution passing through the wall of the water bath, its filling and its cover having an opening above the working area of the chamber, and also a discharge tube for spent saline solution, while the tube for supplying saline solution is made of polyethylene equipped with a glass microcapillary at the outlet located above the hole in the lid of the perfusion chamber, and a discharge tube connects the bottom of the working chamber and through the wall of the water bath to the reservoir aroma of spent saline. Saline from the reservoir, passing through the tube, is heated in the water bath of the perfusion chamber to a predetermined temperature and enters the perfusion chamber for the drug through a glass microcapillary. The spent saline solution leaves the bottom of the perfusion chamber and enters the reservoir through the discharge tube. The advantages of the proposed device perfusion chamber is that excluded the mechanical movement of the microscopic surviving drug in the perfusion chamber, because excluded water hammer drug from the peristaltic pump. 1 ill.

Description

The offer relates to medical equipment.

Known perfusion chambers surviving cell preparation [1-4], containing a tube for supplying saline, passing through the wall of the water bath, filling it, its lid with a hole above the perfusion chamber, as well as a discharge tube for spent saline. Saline suction performs a peristaltic pump. Saline enters the chamber through an opening in the side wall.

The disadvantage of the prototype is the movement of a microscopic cell surviving drug (not fixed, freely lying on a nylon mesh), under the influence of a pump and incoming saline.

The task is to exclude the movement of the microscopic surviving drug in the perfusion chamber, to exclude the water hammer of the drug from the peristaltic pump.

The essence of the proposed device perfusion chamber for surviving microscopic preparation containing a tube for supplying saline solution passing through the wall of the water bath, filling the perfusion chamber and its lid having a hole above the working area of the chamber, as well as a discharge tube for spent saline solution, while the tube for supplying saline solution made of polyethylene, equipped with a glass microcapillary at the outlet located above the hole in the lid of the perfusion chamber, and a discharge tube connects the bottom of the working chamber through the wall of the water bath with a reservoir of spent saline.

Figure 1 shows a schematic representation of the proposed perfusion chamber, where:

1 - saline solution tank

2 - tube for supplying saline

3 - glass microcapillary

4 - water bath

5 - perfusion chamber for the drug

6 - outlet pipe

7 - reservoir of spent saline

8 - water bath cover

Other perfusion chamber systems, as well as filling tanks, tubes and a water bath, are not shown for simplicity.

Saline from the reservoir (1) through the tube (2) is heated in the water bath of the perfusion chamber (4) to a predetermined temperature and enters the perfusion chamber for the preparation through a glass microcapillary (3). The spent saline solution leaves the bottom of the perfusion chamber (5) and enters the reservoir (7) through the tube (6).

The advantages of the proposed perfusion chamber device are that mechanical movements of the microscopic surviving drug in the perfusion chamber are excluded, water hammer of the drug from the peristaltic pump is excluded.

Literature

1. Mityushov M.I., Emelyanov NA, Mokrushin A.A., Voiner I.A., Bagaeva T.R. The surviving section of the brain as an object of neurophysiological and neurochemical research // L .: Nauka, 1986. p.23, 24.

2. Kelso S.R., Nelson D.O., Silva N.L., Boulant J.A. A slice chamber for intracellular and extracellular recording during continuous perfusion // Brain Research Bulletin. 1983. Vol. 10. N6. P.853-857.

3. Shi W.X., Bunney B.S. A small volume chamber for electrical recording from submerged brain slices and a pulse-free medium supply system using a peristaltic pump // Journal of Neuroscience Methods. 1990. Vol. 35. P.235-240.

4. Teyler T.J. Brain slice preparation: hippocampus // Brain Research Bulletin. 1980. Vol. 5. P.391-403.

Claims (1)

A perfusion chamber for a surviving preparation containing a saline solution tube passing through the wall of a water bath, its filling and its cover with an opening above the working chamber, and a discharge tube for spent saline solution, characterized in that the saline solution tube is made of polyethylene and provided at the outlet, a glass microcapillary placed above the hole in the lid of the water bath, the outlet tube connects the hole in the bottom of the working area through the filling and the wall of the water bath with the reservoir for botanical saline.