RU39878U1 - DEVICE FOR FREEZING AND STORAGE OF BIOLOGICAL OBJECTS - Google Patents

Abstract

The utility model relates to medicine, namely to devices for freezing and storing biological objects in a frozen state. The device includes a freezer with refrigerant and contains a labeled basket in the form of a tripod equipped with support strips with horizontal longitudinal protrusions, onto which protruding support rods are vertically stacked, sealed in labeled cryopackages with a frozen biological object, as well as movable inserts that divide the basket into cartridges, and plot for capturing cryo-packaging with forceps. The device can be placed in the freezer of an air electric freezer, or in chilled ethyl alcohol, carbon dioxide (“dry ice”), as well as liquid nitrogen or its vapor. At the same time, the operating temperature range is 22 ° --196 ° С. From the test results it follows that the proposed device is convenient, economical in manufacturing, affordable and can be used with high effect for freezing and frozen hematopoietic stem cells of bone marrow and peripheral blood, platelet concentrates and granulocytes, as well as other media.

Description

The utility model relates to medicine, in particular to devices for freezing and storing biological objects.

A device for freezing and storing biological objects in a frozen state [US PATENTS 3,520,471; 3,576,650; 3,583,460; 3,606,077. HEMOFLEX and UNION CARBIDE], including a freezer with liquid nitrogen and a metal holder immersed in the refrigerant, which is equipped with plates fixing one holder and a container with a bioobject in an upright position.

The known device is inconvenient in operation, excessively consumes usable volume in the freezer and limits the number of biological objects frozen in it, and is also made of expensive material.

Closest to the claimed technical solution is a device for freezing, storing and transporting biological objects [SU A.S. 1399912 A 01 N 1/00, 1985. GU KNIIIGiPK], including a freezer filled with liquid nitrogen, with labeled baskets and cassettes, in which cells are made to accommodate the same size of labeled cryopackages with the main dose and satellites with micro-samples of biological objects in the vertical position.

A disadvantage of the known device is its inability to freeze and store bioobjects in cryo-packaging of different sizes in the freezer, as well as the manufacture of expensive material.

The essence of the development lies in the fact that the basket is made in the form of a tripod, on the upper part of which there are support bars for supporting rods of packages with a bioobject and inserts that divide the basket into

cartridges, while the size of the cartridge may vary from the number and thickness of the installed packages.

Thanks to such design features, the device is convenient in operation, saves the usable volume of the freezer, and thereby allows you to increase the number of frozen bioobjects in cryo packs of different sizes. The use of dense wood simplifies and reduces the cost of manufacturing the device.

The development is described on an example implementation with reference to the accompanying drawing - Figure 1. - diagram of the device.

The device includes a freezer 1 with refrigerant and contains a labeled basket 2 in the form of a tripod, equipped with support strips 3 with horizontal longitudinal protrusions 4, on which the protruding parts 5 of the support rods 6, soldered into labeled cryo packaging 7 with a frozen bioobject, are vertically laid, as well as movable inserts 8, which divide the basket 2 into labeled cassettes 9 of variable length, the space 10 is intended for grippers of forceps.

The device can be placed in the freezer 1 of an air electric freezer, or in chilled ethyl alcohol, carbon dioxide (“dry ice”), a bio-storage with liquid nitrogen or its vapor (temperature 22 ° ÷ 196 ° C).

Cryopack 6 is installed vertically on the support strips 3 on the free space between the inserts 8 of the labeled cartridge 9 of the basket 2.

The proposed development is illustrated by the following examples of its implementation.

Example 1. For the manufacture of the device used blanks from hardwood. External dimensions: 43 × 90 × 43 cm. Weight 2.5 kg. The finished device is placed in an air-cooled freezer 1 in which there is no condensation of water vapor. Protruding parts 5 support

the rod 6 is installed on the horizontal longitudinal projections 4 of the support strips 3. In this case, the cryopacking 7 is vertical. The next cryopackage is installed at a distance of 1 cm from the previous one. The predetermined number of cryopackages 7 in the cassette 9 is controlled by means of movable inserts 8. The cryopackages 7 are removed from the cassette 9 using forceps. To do this, the tongs are held in the space 10 under the support rod 6.

In the process of testing, in all cases, the devices were convenient, they provided vertical and free arrangement of cryopackages 7 in cassette 9, as well as a predetermined mode of freezing and storage of biological objects. The safety of hematopoietic stem cells of bone marrow and peripheral blood, platelet concentrates and granulocytes, according to various assessment methods, is 84%. From the test results it follows that the proposed device can be effective for freezing and frozen hematopoietic stem cells of bone marrow and peripheral blood, platelet concentrates and granulocytes, as well as other media.

Example 2. The device is made of stainless steel or other metals. External dimensions: 43 × 90 × 43 cm. Weight 3.5 kg. The finished device is placed in a freezer 1 with carbon dioxide ("dry ice") at -79 ° C, in which condensation of water vapor is present. The protruding parts 5 of the support rod 6 are installed on the horizontal longitudinal projections 4 of the support strips 3. In this case, the cryopacking 7 is vertical. The next cryopackage is installed at a distance of 1 cm from the previous one. The predetermined number of cryopackages 7 in the cassette 9 is controlled by means of movable inserts 8. The cryopackages 7 are removed from the cassette 9 using forceps. To do this, the tongs are held in the space 10 under the support rod 6.

In the process of testing, in all cases, the devices were convenient, they provided the vertical position of the cryopackages 7, as well as the specified mode of freezing and storage of biological objects. Preservation of hematopoietic stem cells

bone marrow and peripheral blood, platelet concentrates and granulocytes, according to various assessment methods, is 83%. From the test results it follows that the proposed device can be effective for freezing and frozen hematopoietic stem cells of bone marrow and peripheral blood, platelet concentrates and granulocytes, as well as other media.

Example 3. The device is made of stainless steel or other metals. External dimensions: 43 × 90 × 43 cm. Weight 3.5 kg. The finished device is placed in the freezer of the bio-storage 1 with liquid nitrogen at -196 ° C, in which condensate of water vapor is present. The protruding parts 5 of the support rod 6 are installed on the horizontal longitudinal projections 4 of the support strips 3. In this case, the cryopacking 7 is vertical. The next cryopackage is installed at a distance of 1 cm from the previous one. The predetermined number of cryo packages 7 in the cassette 9 is adjusted using movable inserts 8. The cryo packages 7 are removed from the cassette 9 using forceps. For this, nitrogen vapors are sucked out of the bio-storage, the necessary cartridge 9 and cryopacking 7 are found by tags, and the tongs are pulled into the space 10 under the support rod 6.

In the process of testing, in all cases, the devices were convenient, they provided the vertical position of the cryopackages 7, as well as the specified mode of freezing and storage of biological objects. The safety of hematopoietic stem cells of bone marrow and peripheral blood, platelet concentrates and granulocytes, according to various assessment methods, is 87%. From the test results it follows that the proposed device can be effective for freezing and frozen hematopoietic stem cells of bone marrow and peripheral blood, platelet concentrates and granulocytes, as well as other media.

Literature.

1. Preservation of bone marrow at ultra low temperatures with the use of 15% glycerol. Guidelines. M. - 1973 from 10.19.1973

2. Methods of long-term storage in a frozen state of red blood cells intended for transfusion. Guidelines. No. 10-8 / 88. M.-1980 from 12.20.1978

3. Guidelines for transfusion medicine (Ed. By E. P. Swedentsov. - Kirov, 199.-716 p.).

Claims (1)

A device for freezing and storing biological objects, including a freezer, labeled baskets and cassettes with cells for vertical placement of labeled containers with the main dose and micro samples of a biological object, characterized in that the basket is made in the form of a tripod, on the upper part of which there are support plates for supporting rods of packages with a biological object, and inserts dividing the basket into cartridges, while the size of the cartridge varies from the number and thickness of the installed packages.