RU2818047C2 - Optical radiation source for exciting fluorescence, raman scattering in analytical and bioanalytical equipment - Google Patents

Abstract

FIELD: biotechnology.

SUBSTANCE: invention relates to analytical and bioanalytical equipment used in various applied biological and biomedical research. Optical radiation source for fluorescence excitation, Raman scattering in analytical and bioanalytical equipment comprises a laser video projector, which controls laser radiation in accordance with a video signal received from a control computer, laser video projector is connected to a control computer through a feedback system including a camera receiving optical radiation through a focusing optical system. To correct optical radiation parameters, the control computer comprises a program for controlling optical radiation of the laser video projector through a video stream transmitted to the laser video projector. Laser video projector is in the form of a laser scanning video projector or a laser matrix video projector.

EFFECT: invention provides excitation of fluorescence, Raman scattering in analytical and bioanalytical equipment.

10 cl, 6 dwg

Description

Field of technology

The invention relates to analytical and bioanalytical equipment, applicable in various applied biological and biomedical research. The source of optical radiation for excitation of fluorescence, Raman scattering and other optical signals is suitable for use in analytical and bioanalytical equipment, as well as in other optical instruments, including, but not limited to, microscopy and image analysis systems, flow cytometry, spectroscopy, immunoglobulin scanning and DNA matrices, 3D printers, machine vision systems.

State of the art

Optical emission: decohered and narrowband light containing one or more spectral bands, modulated light with a homogenized light beam profile - required in a variety of optical devices, including, but not limited to, microscopy and imaging systems, flow cytometry, spectroscopy, immunoglobulin scanning and DNA matrices, 3D printers, machine vision systems.

Known technical solutions for producing optical radiation suitable for excitation of fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment include a block of laser emitters or a block of LED emitters, a unit for homogenization or other profiling of optical radiation (for example, a P-shater), and a compensation unit thermal drift, decogeneration unit and interface unit in various combinations. These are technically complex solutions, the cost of such solutions is tens of thousands of dollars or more. However, the known devices have limited capabilities for adjusting the parameters of the resulting optical radiation in real time.

A known microchip analyzer based on wide-field fluorescence microscopy with laser illumination and a device for speckle suppression, providing limited decoherence of the beam using optical tubes and rotating mirrors (Source [1]: Microchip analyzer based on wide-field fluorescence microscopy with laser illumination and a device for speckle suppression. November 2017. Biomedical Optics Express 8(11):4798 DOI:10.1364/BOE.8.004798. [Microarray analyzer based on wide field fluorescent microscopy with laser illumination and a device for speckle suppression. November 2017. Biomedical Optics Express 8(11): 4798 DOI:10.1364/BOE.8.004798 Authors:Yuri Lysov, Victor Barsky Dmitriy Urasov, Roman Urasov]. Access mode: https://www.researchgate.net/publication/320207384_Microarray_analyzer_based_on_wide_field_fluorescent_microscopy _with_laser_illumination_and_a_device_for_ speckle_suppression). The analyzer contains lasers for excitation of fluorescence, barrier filters, optics for projecting images onto an image detector, and a device for suppressing laser spots on the microchip substrate. The speckle suppression device contains a fiber-optic beam and a rotating mirror located in such a way as to change the distance between the end of the beam and the mirror surface during each revolution of the mirror.

It does not allow the creation of decohered and narrow-band, containing one or more spectral bands, modulated light (optical radiation) with a homogenized, dynamically tunable light beam profile for excitation of fluorescence, Raman scattering and other optical signals.

Does not allow the creation of optical systems requiring homogenized and/or decohered and/or narrowband and/or multiband and/or pulsed and/or modulated and/or dynamically tunable optical radiation in a cost-effective and/or portable and/or energy-saving format, including but not limited to not limited to, microscopy and image analysis systems, flow cytometry, spectroscopy, scanning of immunoglobulin and DNA matrices, 3D printers, machine vision systems.

The known module for compensation of thermal drift in cytometry (Source [2]: Laser beam stabilizer. Access mode: https://www.kineticriver.com/beam-genie/) actively compensates for mismatch of the laser beam due to thermal shifts in laser aiming, providing correct aiming of the laser beam at the intended target.

A homogenization module (P-shaper) is known (Source [3]: Flat Top Beam Shapers [AdlOptica πShaper Flat Top Beam Shapers]. Access mode: https://www.edmundoptics.com/f/flat-top-beam-shapers/15036 /). These are refractive field imaging optical systems that convert collimated input Gaussian beams into collimated plane beams with a uniform intensity distribution and a flat phase front. Thanks to the optical field mapping design, the uniform intensity distribution of the converted beam is stable over long distances, making it suitable for holography, microscopy and system integration.

The functionality does not involve configuration or dynamic adjustment.

The ability to freely and dynamically adjust the radiation intensity in the beam cross section is achievable using both arrays of micromirrors (DLP) and scanning micromechanical devices (MEMS), as well as arrays of liquid crystal cells and similar systems, however, such solutions, manufactured individually, require specialized interfaces, and require optimization for mass use.

Solutions that include separately implemented static P-shapers, various decoherence and modulation systems, systems for compensation of thermal, mechanical and other types of drift are limited in functionality and require optimization for mass use.

The units for generating, focusing and combining radiation of different wavelengths are bulky in comparison with the components of custom projectors optimized in mass production and require optimization for mass use.

Homogenized and/or decohered and/or narrowband and/or multiband and/or pulsed and/or modulated and/or dynamically tunable optical radiation in a cost-effective and/or portable and/or energy-efficient format is required in a variety of optical devices, including but not limited to microscopy and image analysis systems, flow cytometry, spectroscopy, scanning of immunoglobulin and DNA matrices, 3D printers, machine vision systems.

The problem is that the prior art does not know a device that provides homogenized and/or decohered and/or narrowband and/or multiband and/or pulsed and/or modulated and/or dynamically tunable optical radiation in an economical and/or portable and/ or in an energy-saving format suitable for mass, cost-effective use for analytical and bioanalytical equipment.

The essence of the invention.

The technical result consists in ensuring the excitation of fluorescence, Raman scattering and other optical signals in analytical and bioanalytical equipment, as well as in other optical instruments, including, but not limited to, microscopy and image analysis systems, flow cytometry, spectroscopy, immunoglobulin and DNA scanning - matrices, 3D printers, machine vision systems. The technical solution provides homogenized and/or decohered and/or narrowband and/or multiband and/or pulsed and/or modulated and/or dynamically tunable optical radiation in an economical and/or portable and/or energy-saving format, suitable for mass, cost-effective use for analytical and bioanalytical equipment.

The technical result is achieved in that the source of optical radiation for excitation of fluorescence and Raman scattering in analytical and bioanalytical equipment contains a laser video projector that controls laser radiation in accordance with the video signal received from the control electronic computer; the laser video projector is connected to the control electronic computer through a feedback system including a camera receiving optical radiation through a focusing optical system for adjusting the parameters of optical radiation; the control electronic computer contains a program for controlling the optical radiation of a laser video projector by means of a video stream transmitted to the laser video projector, wherein the laser video projector is made in the form of a laser scanning video projector or in the form of a laser matrix video projector.

It is envisaged that the laser video projector in the optical radiation source includes a block of collimated lasers and a scanning microelectromechanical MEMS block.

It is envisaged that the control electronic computer in the optical radiation source is made in the form of a smartphone or tablet computer.

It is provided that in the optical radiation source, the focusing optical system includes a cylindrical optical lens that equalizes the divergence angle vertically/horizontally, in combination with a spherical lens that focuses the radiation flux into the beam.

It is provided that in the source of optical radiation, the focusing optical system includes a lens with curvature differing along the axes, which simultaneously equalizes the divergence angle vertically/horizontally and focuses the radiation flux into the beam.

It is provided that in the optical radiation source, the focusing optical system includes apochromatic optics designed to focus radiation without introducing chromatic distortions when using radiation of more than one wavelength.

It is provided that in the optical radiation source, the focusing optical system contains optics installed using electromechanical actuators, tunable for each sequentially used wavelength.

It is provided that in the optical radiation source the feedback system includes a beam splitter that redirects part of the optical radiation to a video camera designed to record the optical radiation reflected from the working surface, in parallel with fluorescence detection.

It is envisaged that the feedback system in the optical radiation source includes point detectors designed for high-speed detection of radiation modulation and pulse parameters.

The technical result is achieved by the fact that in the method of controlling the intensity of radiation in the cross section of a beam generated by a source of optical radiation to excite fluorescence, Raman scattering in analytical and bioanalytical equipment, the image transmitted to the laser video projector is corrected or the brightness of the image transmitted to the laser video projector is adjusted in each of the colors, the formation of radiation carried out by broadcasting a series of images to a laser video projector.

The device and method provide homogenized and/or decohered and/or narrowband and/or multiband and/or pulsed and/or modulated and/or dynamically tunable optical radiation in an economical and/or portable and/or energy-saving format.

The invention is illustrated by graphic materials:

Figure 1 – diagram, source of excitation of fluorescence, Raman scattering and other optical signals for use in epifluorescence microscopy in transmitted mode.

Fig. 2 – diagram, source of excitation of fluorescence, Raman scattering and other optical signals for use in epifluorescence microscopy (including for scanning analytical matrices) in transmitted mode, equipped with a filter 3, a collecting optical system 4 and a camera 5;

Figure 3 is a diagram, an example of focusing the radiation of a projector 1 and the organization of a feedback system 7 through the camera 5 of the control computer, made in the form of a smartphone. Cylindrical and spherical lenses, or one lens with curvature differing along the axes (or other similar optics) generates a flux of optical radiation with the same parameters of convergence or divergence along both axes. This flow can be converted into a parallel flow by an additional spherical lens (or other similar optics). A small part of the beam (for example, ~ 6% when using a glass slide at an angle of 45 degrees) is directed by a divider to camera 5 of the control computer to provide feedback (camera 5 is combined with the computer, i.e. a smartphone or tablet PC is used).

Figure 4 is a diagram, an example of focusing radiation from a source of fluorescence excitation, Raman scattering and other optical signals for analytical and bioanalytical equipment onto a protein or DNA matrix (sample 2) with fluorescent indication of the binding of the analyzed molecules, and epifluorescent reading of the generated signal by camera 5 controlling the projector 1 computer (in the form of a smartphone). The size of the matrix (item 2) in the image is exaggerated for clarity. Calibration of the exciting radiation to compensate for angular distortions, temperature and other drift, and inhomogeneity of the beam profile can be realized both by analyzing the beam itself (as shown in Fig. 3) and by analyzing the image it forms, which can be read, in particular, at the service wavelength (e.g. red), while the reading of the fluorescent signal is at the working length(s), e.g. blue/green and/or green/orange. Consistent calibration and reading simplifies device design. Targeted illumination of only the binding regions of the analyzed molecules improves the signal-to-noise ratio. Modulation of the excitation radiation followed by analysis of the modulation of the detected signal (e.g. Fourier analysis) improves the signal-to-noise ratio;

Figure 5 is a diagram, an example of focusing radiation from a source of fluorescence excitation, Raman scattering and other optical signals for analytical and bioanalytical equipment onto a protein or DNA matrix (sample 2) with a fluorescent indication of the binding of the analyzed molecules, and scanning reading of the generated signal when moving the matrix, controlled by smartphone. Calibration of the exciting radiation to compensate for angular distortions, temporal drift, and correction of the beam profile can be implemented both by analyzing the beam itself (as shown in Fig. 3) and by analyzing the image it generates during scanning, which can be read, in particular, at the service wavelength (e.g. red), while the reading of the fluorescent signal is at the working length(s), e.g. blue/green. Modulation of the excitation radiation followed by analysis of the modulation of the detected signal (e.g. Fourier analysis) improves the signal-to-noise ratio;

Figure 6 is a diagram of an example of apochromatic focusing of radiation from a fluorescence excitation source, Raman scattering and other optical signals for analytical and bioanalytical equipment onto a flow detection cell.

The numbers on the graphic materials indicate the following positions:

1 - projector;

2 - sample;

3 - filter;

4 - optical system;

5 - camera;

6 - electronic computer (computer);

7 - feedback system;

8 - beam divider;

9 - lenses.

Carrying out the invention

It is envisaged to use a laser video projector controlled by an electronic computer as a source of optical radiation to excite fluorescence, Raman scattering and other optical signals in analytical and bioanalytical equipment, microscopy and image analysis systems, flow cytometry systems, spectroscopy, immunoglobulin and DNA scanning. matrices, 3D printers, machine vision systems.

The source of optical radiation for analytical and bioanalytical equipment contains a laser video projector that controls laser radiation in accordance with the video signal received from the control electronic computer.

The laser projector is connected to an electronic computer through a feedback system and includes a camera that receives optical radiation through the optical system to adjust the parameters of the optical radiation.

The projector includes a collimated laser unit and a scanning microelectromechanical MEMS unit.

The control electronic computer is made in the form of a smartphone or tablet computer with a video camera.

The source of excitation of fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment contains a laser scanning video projector, an optical system for focusing radiation, a feedback system, an electronic computer with a program for controlling the optical radiation of a video projector transmitted to the projector video stream.

The source of excitation of fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment contains a laser matrix video projector, an optical system for focusing radiation, a feedback system, an electronic computer with a program for controlling the optical radiation of a video projector via a video stream transmitted to the projector.

The focusing optical system includes a cylindrical optical lens that levels the divergence angle vertically/horizontally, in combination with a spherical lens/lenses that focuses the radiation flux into a beam of the required parameters.

The focusing optical system includes a lens with a different curvature along the axes, which simultaneously equalizes the divergence angle vertically/horizontally and focuses the radiation flux into a beam of the required parameters.

The focusing optical system includes apochromatic optics, which focuses radiation without introducing chromatic distortion when using radiation of more than one wavelength.

The focusing optical system includes optics tunable for each sequentially used wavelength using electromechanical actuators.

The feedback system includes a beam splitter that redirects part of the optical radiation to the video camera.

The feedback system includes a video camera that records optical radiation reflected from the working surface, in parallel with the detection of fluorescence or other optical signals.

The feedback system includes one that registers radiation passing through the working area at reference points or temporally separated from the analyzed objects.

The feedback system includes point detectors for high-speed detection of radiation modulation and pulse parameters.

A method of using a source of excitation of fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment for its intended purpose, in which, to control the intensity of the generated radiation in the cross section of the beam, the image transmitted to the projector is corrected or the brightness of the image transmitted to the projector is adjusted in each of the colors, the formation of radiation is carried out by broadcast a series of images on a laser video projector.

As a source of optical radiation for analytical and bioanalytical equipment, it was envisaged to use a laser video projector controlled by an electronic computer.

The use of a mass-produced laser projector is proposed for use as a source of optical radiation, in analytical and bioanalytical equipment, to produce optical radiation: decohered and narrow-band light containing one or more spectral bands, modulated light with a homogenized light beam profile - required in a variety of optical devices , including, but not limited to, microscopy and image analysis systems, flow cytometry, spectroscopy, scanning of immunoglobulin and DNA matrices, 3D printers, machine vision systems.

The use of a mass-produced laser projector increases the energy efficiency of equipment, allows for reduction in size (compactness), reduces the cost of analytical and bioanalytical equipment, and expands its availability to a wide range of researchers.

A method of using a laser video projector controlled by an electronic computer as a source of optical radiation for analytical and bioanalytical equipment.

The source of excitation of fluorescence, Raman scattering and other optical signals (optical radiation) for analytical and bioanalytical equipment produces homogenized and/or decohered and/or narrowband and/or multiband and/or pulsed and/or modulated and/or dynamically tunable optical radiation in an economical manner and/or portable and/or energy-saving format.

Such optical radiation is required in a variety of optical devices, including, but not limited to, microscopy and imaging systems, flow cytometry, spectroscopy, immunoglobulin and DNA matrix scanning, 3D printers, and machine vision systems.

The source of excitation of fluorescence, Raman scattering of light and other optical signals for analytical and bioanalytical equipment contains a projector 1 (Fig. 1; Fig. 2; Fig. 3; Fig. 4; Fig. 5; Fig. 6), controlling laser beams of three wavelengths in accordance with the received video signal. The projector 1 (for example, a laser pico projector or a MEMS laser scanning projector) includes a collimated laser unit and a scanning microelectromechanical unit (MEMS unit), controlled via a video interface. Laser sources, combined in projector 1 into a common beam with dichroic mirrors, provide a narrowband of each of the three radiation bands, and a high spectral density of radiation with minimal energy consumption.

Projector 1 can be supplemented with the following elements in various combinations:

- focusing optical system 4, leveling the divergence angle of optical radiation vertically/horizontally (for example, 40 and 22.5 degrees in the above example are leveled to 22.5 degrees along two axes);

- focusing optical system 4, forming optical radiation into a beam of the required geometry (including a parallel beam, converging or diverging flow);

- a control device that generates a video stream in hardware, made on the basis of a computer 6;

- control software used to control the computer 6, which programmatically generates the video stream;

- feedback system 7, which provides signals for the source to correct the beam profile, spectral components of the beam, beam power, beam drift, beam modulation and other parameters of optical radiation.

Projector 1 controls laser beams of three wavelengths, in accordance with the video signal received through the video interface from the control device, allows you to create decohered and narrow-band light containing one or more spectral bands, modulated light (optical radiation) with a homogenized light beam profile, or any other, including a dynamically tunable light beam profile, which is a source of excitation of fluorescence, Raman scattering of light and other optical signals.

A method is provided for using a source of excitation of fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment to control the intensity of the generated radiation in the beam cross section by means of correction transmitted to the image projector 1.

A method is provided for using a source of excitation of fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment to control the intensity of the generated radiation depending on the time of illumination, temperature and other factors influencing the radiation parameters - by correcting the brightness of the image transmitted to the projector in each color .

A method is provided for using a source of excitation of fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment with a video camera, including one combined with a computer 6 (e.g. smartphone, tablet, laptop) control video projector 1 for organizing feedback to control the intensity of the generated radiation in beam cross section and over time, by correcting the image transmitted to the projector.

A method is provided for using a source of excitation of fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment based on a video projector containing a scanner or DLP and LCD matrices for beam decohering and speckle suppression. For example, for scanning with a laser beam focused from the radiation of the above-mentioned projector with a resolution of 1280x720 = 921 thousand pixels and a refresh rate of 60fps, with an exposure time of 1 s, the radiation is divided into 5.5 million elements, causing almost complete decoherence.

A method is provided for using a source of excitation of fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment based on a video projector 1 in which the formation of radiation is carried out by broadcasting a series of images to a scanning laser video projector 1 to modulate the radiation at frequencies of 30-120 Hz, and using them together with graphical, pixel-by-pixel image encoding for scanning projectors to modulate focused radiation at megahertz frequencies and/or produce picosecond pulses. For example, alternating light and dark pixels in the image scan of the above projector with a resolution of 1280x720 = 921 thousand pixels at a frequency of 60 fps will lead to the generation of 460,500x60 = 27,630,000 pulses per second. Accordingly, the minimum pulse length is 1/(921000*60)=18 ps.

A method is provided for using a source of excitation of fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment, which proposes targeted illumination of only microzones for detecting biomolecules in epifluorescent mode, set by broadcasting the corresponding image to the projector, to increase the signal-to-noise ratio.

A method is provided for using a source of excitation of fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment based on a video projector containing a block of three collimated lasers and a corresponding interface to generate narrow-band radiation at one, two or three wavelengths, with a high degree of control of the generated radiation in the beam cross section by correcting the image transmitted to the projector.

A method is provided for using a source of excitation of fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment based on a video projector containing a block of three collimated lasers and a corresponding interface to generate one, two, and three-band radiation, with a high degree of control of the generated radiation in the cross section beam by correcting the image transmitted to the projector.

A method is provided for using a source of excitation of fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment based on a video projector containing a block of three collimated lasers, supplemented by additional lasers sequentially controlled through a built-in interface, to generate multi-band radiation, with a high degree of control of the generated radiation in the cross section of the beam by correcting the image transmitted to the projector.

Example 1. Source of excitation of fluorescence, Raman scattering and other optical signals for use in epifluorescence microscopy in transmitted mode (Fig. 1).

The source of excitation of fluorescence, Raman scattering of light and other optical signals for use in epifluorescence microscopy contains a projector 1 that controls laser beams of three wavelengths, in accordance with a video signal received through a video interface from a control device made in the form of an electronic computer 6 (Fig. 1), which generates a video stream for analysis of sample 2. The video stream is generated using video cards and corresponding software.

Example 2. Source of excitation of fluorescence, Raman scattering and other optical signals for use in epifluorescence microscopy in transmitted mode (Fig. 2). additionally equipped with a filter 3, a collecting optical system 4 and a camera 5 (Fig. 2). Camera 5 (video or photo camera) is used to detect the signal from the analyzed sample 2, and to organize feedback from the projector 1 through the video interface from the control electronic computer 6 (Fig. 1) and the feedback system 7. The light-transmitting sample 2 is placed at a distance (required by the geometry of the illuminated area) from the projector scanner 1. The optical radiation parameters are controlled through a standard video interface, for example HDMI in the example given. The video stream is generated using video cards and corresponding software.

Example 3. A source for excitation of fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment, for universal purposes, contains a video projector 1 (for example, a USB-powered laser scanning projector is used, compatible with HDMI devices, iPhone, iPad and Android phones) including a block of three collimated lasers and a scanning microelectromechanical block (MEMS block), controlled by a computer 6 via a video interface and complemented by a focusing optical system 4, leveling the divergence angle vertically/horizontally (for example, 40 and 22.5 degrees in the above example are leveled to 22.5 degrees along two axes), as well as focusing optical radiation into a beam of the required geometry, including a parallel beam, converging or diverging flow; The computer 6 controls the proctor 1 through the feedback system, the feedback system 7 provides the signals (information) necessary to adjust the beam profile, spectral components of the beam, beam power, beam drift, beam modulation and other parameters generated by the projector 1 (Fig. 3). Optical radiation parameters are controlled through a standard video interface, for example HDMI in the example given. The video stream is generated using video cards and corresponding software.

The focusing optical system 4 provides the following private options, which serve only to illustrate private options of the invention, but do not limit other possible embodiments of the invention, available to specialists in various combinations:

- the focusing optical system 4 includes a cylindrical optical lens (or other optics similar in parameters), leveling the divergence angle vertically/horizontally, in combination with a spherical lens/lenses (or other optics similar in parameters), focusing the radiation flux into the beam required parameters;

- the focusing optical system 4 includes a lens with a different curvature along the axes (or other optics with similar parameters), which simultaneously equalizes the divergence angle vertically/horizontally and focuses the radiation flux into a beam of the required parameters;

- the focusing optical system 4 includes apochromatic optics that performs the described focusing without introducing chromatic distortion when using radiation of more than one wavelength;

- focusing optical system 4 includes optics tunable for each sequentially used wavelength using electromechanical actuators;

Figure 3 shows an example of focusing the radiation of the projector 1 and the organization of the feedback system 7 through the camera 5 of the control computer 6, made in the form of a smartphone. Cylindrical and spherical lenses, or one lens with curvature differing along the axes (or other similar optics) generates a flux of optical radiation with the same parameters of convergence or divergence along both axes. This flow is converted into a parallel flow by an additional spherical lens (or other similar optics). A small part of the beam (for example, ~ 6% when using a glass slide at an angle of 45 degrees) is directed by a divider to camera 5 of the control computer to provide feedback (camera 5 is combined with the computer, i.e. a smartphone or tablet PC is used).

Feedback system 7 provides the following private options, which serve only to illustrate private options of the invention, but do not limit other possible embodiments of the invention, available to specialists in various combinations:

- the feedback system 7 includes a beam splitter 8, which redirects a small part of the optical radiation to the video camera 5, with the optional use of lenses 9 of the focusing optical system 4;

- the feedback system 7 includes a video camera 5 that records optical radiation reflected from the working surface, in parallel with the detection of fluorescence or other optical signals - for example, in systems of microscopy and image analysis, spectroscopy, scanning of immunoglobulin and DNA matrices (Fig. 4) , 3D printers, machine vision systems;

- the feedback system 7 includes a video camera 5 that records radiation passing through the working area at reference points or temporally separated from the analyzed objects 2 - for example, in microscopy and image analysis systems, flow cytometry (Fig. 5), spectroscopy, immunoglobulin scanning and DNA matrices, 3D printers, machine vision systems;

- feedback system 7 includes augmented point detectors for high-speed detection of radiation modulation and pulse parameters.

Optical radiation parameters are controlled via a standard video interface, such as HDMI. The video stream is generated using video cards and corresponding software.

The laser illumination zone is formed from many (1280x720=921 thousand in the given example) pixels, sequentially updated at least with the frame rate of the video stream (60 Hz in the given example), which effectively decoheres the radiation and suppresses speckles.

Independent control of the intensity of each pixel at each wavelength is implemented by programming the video stream, and allows you to homogenize or customize the radiation in the beam cross-section, as well as modulate the radiation across the entire beam cross-section in real time.

Laser sources, combined in projector 1 into a common beam with dichroic mirrors, provide a narrowband of each of the three radiation bands, and a high spectral density of radiation with minimal energy consumption.

Example 4. The use of the invention for epifluorescence reading of a protein or DNA matrix with fluorescent indication of the binding of the analyzed molecules (or in other epifluorescence microscopy) is shown in Fig. 4.

The source of excitation of fluorescence, Raman scattering and other optical signals for obtaining homogenized and/or decohered and/or narrowband and/or multiband and/or pulsed and/or modulated and/or dynamically tunable optical radiation contains a laser scanning video projector 1 (Fig. .4), an optical system 4 for focusing radiation, a feedback system 7, a computer 6 with a program for controlling the optical radiation of the video projector 1 by means of a video stream transmitted to the projector 1.

In fig. Figure 4 shows an example of focusing radiation from a fluorescence excitation source, Raman scattering and other optical signals for analytical and bioanalytical equipment onto a protein or DNA matrix (sample 2) with a fluorescent indication of the binding of the analyzed molecules, and epifluorescent reading of the generated signal by camera 5 controlling computer projector 1 (in in the form of a smartphone). The size of the matrix (item 2) in the image is exaggerated for clarity. Calibration of the exciting radiation to compensate for angular distortions, temperature and other drift, and inhomogeneity of the beam profile can be realized both by analyzing the beam itself (as shown in Fig. 3) and by analyzing the image it forms, which can be read, in particular, at the service wavelength (e.g. red), while the reading of the fluorescent signal is at the working length(s), e.g. blue/green and/or green/orange. Consistent calibration and reading simplifies device design. Targeted illumination of only the binding regions of the analyzed molecules improves the signal-to-noise ratio. Modulation of exciting radiation followed by analysis of modulation of the detected signal

Example 5. The use of the invention in scanning reading of a protein or DNA matrix with fluorescent indication of the binding of the analyzed molecules (or in other scanning fluorescence microscopy) is shown in Fig. 5.

The source of excitation of fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment to obtain homogenized and/or decohered and/or narrowband and/or multiband and/or modulated and/or dynamically tunable optical radiation, contains laser, matrix (DLP, LCD ) video projector 1, optical system 4, for focusing radiation, feedback system 7, computer 6 with a program for controlling the optical radiation of video projector 1 by means of a video stream transmitted to projector 1.

In contrast to the source (example 4) based on scanning projectors 1, sources (example 5) based on laser matrix projectors 1 can use simpler optical systems 4 for focusing due to the identical divergence angles of the radiation horizontally and vertically. Also, matrix systems are limited in modulation and available pulse lengths compared to scanning systems.

An example of focusing the radiation of a fluorescence excitation source, Raman scattering and other optical signals for analytical and bioanalytical equipment onto a protein or DNA matrix (sample 2) with a fluorescent indication of the binding of the analyzed molecules, and scanning reading of the generated signal when moving the matrix controlled by a smartphone. Calibration of the exciting radiation to compensate for angular distortions, temporal drift, and correction of the beam profile can be implemented both by analyzing the beam itself (as shown in Fig. 3) and by analyzing the image it generates during scanning, which can be read, in particular, at the service wavelength (e.g. red), while the reading of the fluorescent signal is at the working length(s), e.g. blue/green. Modulation of the excitation radiation followed by analysis of the modulation of the detected signal (e.g. Fourier analysis) improves the signal-to-noise ratio.

An example of the application of the invention for flow analysis of microparticles (cytometry) is shown in FIG. 6. An example of apochromatic focusing of radiation from a fluorescence excitation source, Raman scattering and other optical signals for analytical and bioanalytical equipment onto a flow detection cell.

For the given examples of implementation of the invention, it is assumed that fluorescence, Raman scattering and other optical signals for analytical and bioanalytical equipment, video projectors containing a block of three collimated lasers, or a block of three collimated lasers, with the replacement of one or more lasers with a laser of a different length, are used in the source of excitation waves or a block of three collimated lasers, with the block complemented by one or more lasers with a different wavelength when using the built-in interface. Provision is made for alternating control of a pair or more lasers by one interface channel, implemented through a sequence of frames in a video stream, sequentially controlling several lasers through one transmitted color.

It is stipulated that the source uses a mass-produced scanner block for video projectors and its corresponding interface, uses a mass-produced DLP block for video projectors and its corresponding interface, and uses a mass-produced LCD block and its corresponding interface.

It is envisaged that the source uses a mass-produced interface for video projectors, using a video stream generated in hardware by mass-produced video cards.

The source is intended to use a mass-produced interface for video projectors that uses a video stream generated programmatically using software developed for media applications. This solution is unique in price, compactness and ease of use.

It is envisaged that the source uses mass-produced video cameras that generate a video stream for use in the source feedback system.

The invention can bring the greatest expected industrial (economic, technical) effect in the segment of mass-produced and portable analytical and bioanalytical equipment. The use will have a positive impact on the saturation of the market for analytical equipment and, as a result, increasing the overall technological level of society.

The source of excitation of fluorescence, Raman scattering of light and other optical signals for analytical and bioanalytical equipment produces homogenized and/or decohered and/or narrowband and/or multiband and/or pulsed and/or modulated and/or dynamically tunable optical radiation in an economical and/or portable and/or energy-saving format. Such optical radiation is required in a variety of optical devices, including, but not limited to, microscopy and imaging systems, flow cytometry, spectroscopy, immunoglobulin and DNA matrix scanning, 3D printers, and machine vision systems.

The device provides homogenized and/or decohered and/or narrowband and/or multiband and/or pulsed and/or modulated and/or dynamically tunable optical radiation in an economical and/or portable and/or energy-saving format.

Homogenized and/or decohered and/or narrowband and/or multiband and/or pulsed and/or modulated and/or dynamically tunable optical radiation in a cost-effective and/or portable and/or energy-saving format is required in a variety of optical devices, including but not limited to systems microscopy and image analysis, flow cytometry, spectroscopy, scanning of immunoglobulin and DNA matrices, 3D printers, machine vision systems.

Devices that provide a combination of the listed properties for analytical and bioanalytical equipment are currently not available on the market.

The solution, for the declared set of properties, is unique in price, compactness and energy efficiency, since it contains the most complex blocks (emitters, scanner, interface) optimized for mass production.

Claims (10)

1. A source of optical radiation for excitation of fluorescence, Raman scattering in analytical and bioanalytical equipment, characterized in that it contains a laser video projector that controls laser radiation in accordance with the video signal received from the control electronic computer, the laser video projector is connected to the control electronic computer through a feedback system, including a camera receiving optical radiation through a focusing optical system, to adjust the parameters of optical radiation, the control electronic computer contains a program for controlling the optical radiation of a laser video projector by means of a video stream transmitted to the laser video projector, wherein the laser video projector is made in the form of a laser scanning video projector or in the form of a laser matrix video projector. 2. The optical radiation source according to claim 1, characterized in that the laser video projector includes a block of collimated lasers and a scanning microelectromechanical MEMS block. 3. The source of optical radiation according to claim 1, characterized in that the control electronic computer is made in the form of a smartphone or tablet computer. 4. The source of optical radiation according to claim 1, characterized in that the focusing optical system includes a cylindrical optical lens that aligns the divergence angle vertically/horizontally, in combination with a spherical lens that focuses the radiation flux into the beam. 5. The source of optical radiation according to claim 1, characterized in that the focusing optical system includes a lens with a different curvature along the axes, which simultaneously equalizes the divergence angle vertically/horizontally and focuses the radiation flux into the beam. 6. An optical radiation source according to claim 1, characterized in that the focusing optical system includes apochromatic optics designed to focus radiation without introducing chromatic distortion when using radiation of more than one wavelength. 7. The optical radiation source according to claim 1, characterized in that the focusing optical system contains optics installed using electromechanical actuators, tunable for each successively used wavelength. 8. The source of optical radiation according to claim 1, characterized in that the feedback system includes a beam splitter that redirects part of the optical radiation to a video camera designed to record optical radiation reflected from the working surface, in parallel with fluorescence detection. 9. The optical radiation source according to claim 1, characterized in that the feedback system includes point detectors designed for high-speed detection of radiation modulation and pulse parameters. 10. A method for controlling the intensity of radiation in the cross section of a beam generated by a source of optical radiation to excite fluorescence, Raman scattering in analytical and bioanalytical equipment described in claim 1, in which the image transmitted to the laser video projector is adjusted or the brightness of the image transmitted to the laser video projector is adjusted in each from flowers, the formation of radiation is carried out by transmitting a series of images to a laser video projector.