RU2797537C2 - Methods and compositions for improving plant characteristics - Google Patents

Abstract

FIELD: biotechnology.

SUBSTANCE: method for identifying a microorganism strain that produces at least one pound of nitrogen per acre per season is proposed. The method includes the analysis of two or more strains of microorganisms-candidates for colonizing ability and nitrogen production per microorganism per hour. The following is identified: at least one strain of microorganisms from these two or more strains of candidate microorganisms, which have a product of (i) colonizing ability and (ii) N produced per microorganism per hour of at least about 2.5 × 10^-8 mmol N per gram wet weight of plant root tissue per hour. At the same time, at least one strain of microorganisms from these two or more strains of candidate microorganisms have an average colonizing ability per unit of plant root tissue of at least 1.79 × 10⁵ bacterial cells per gram wet weight of plant root tissue. The identified strain with the specified characteristics of (i) colonizing ability and (ii) N produced per microorganism per hour is used in a method to increase nitrogen fixation in non-legume plants in the field, to colonize a non-legume crop, to obtain a composition for increasing the yield of a non-legume crop.

EFFECT: invention provides an increase in nitrogen fixation in non-legume plants.

29 cl, 41 dwg, 22 tbl, 13 ex

Description

CROSS REFERENCES

[0001] This application claims the priority of US Provisional Application No. 62/445570 filed January 12, 2017; U.S. Provisional Application No. 62/445557, filed January 12, 2017; U.S. Provisional Application No. 62/447889, filed January 18, 2017; U.S. Provisional Application No. 62/467032, filed March 3, 2017; U.S. Provisional Application No. 62/566199, filed September 29, 2017, and U.S. Provisional Application No. 62/577147, filed October 25, 2017, which are incorporated herein by reference.

INFORMATION REGARDING THE SEQUENCE LIST

[0002] This application contains a sequence listing that is filed electronically in ASCII format and is therefore incorporated by reference in its entirety. The specified ASCII copy, created on January 3, 2018, is named 47736-707_601_SL.txt and is ≈599 KB in size.

FEDERALLY FUNDED STUDY STATEMENT

[0003] The present invention was made with the support of the Government of the United States of America in the form of grant SBIR 1520545 allocated by the National Science Foundation. The US government has certain rights in the subject matter described.

BACKGROUND OF THE INVENTION

[0004] Plants are associated with the microbiome by a common metabolome. The multidimensional relationship between a particular trait of an agricultural crop and its underlying metabolome is characterized by a relief with many local maxima. Optimizing from a smaller local maximum to another representing an improved trait by changing the influence of the microbiome on the metabolome may be desirable for a variety of reasons, such as crop optimization. Economically, environmentally and socially sustainable approaches to agriculture and food production are needed to meet the needs of a growing global population. The Food and Agriculture Organization of the United Nations predicts that to meet the needs of a growing population by 2050, total food production must increase by 70%, a task that is complicated by numerous factors, including declining fresh water resources, increasing competition for arable land, rising energy prices, rising production costs and similar demands to adapt crops to the pressures of a drier, hotter and more extreme global climate.

[0005] One area of interest is improving nitrogen fixation. Gaseous nitrogen (N ₂ ) is the main component of the Earth's atmosphere. In addition, elemental nitrogen (N) is an essential component of many of the chemical compounds that make up living organisms. However, many organisms cannot directly use _N2 to synthesize chemical compounds used in physiological processes such as growth and reproduction. To use N ₂ it is necessary that N ₂ be combined with hydrogen. The combination of hydrogen with N ₂ is referred to as nitrogen fixation. Nitrogen fixation, either chemically or biologically, requires a large amount of energy. In biological systems, the reaction that leads to nitrogen fixation is catalyzed by an enzyme known as nitrogenase. An important goal of nitrogen fixation research is to extend this phenotype to non-legumes, specifically to important agricultural grasses such as wheat, rice, and corn. Despite great progress in understanding the development of nitrogen-fixing symbiosis between nodule bacteria and legumes, the way to use this knowledge to induce nitrogen-fixing nodules in non-legumes is still unclear. Meanwhile, the challenge of providing sufficient additional sources of nitrogen, such as in fertilizers, will continue to grow along with the growing need to increase food production.

SUMMARY OF THE INVENTION

[0006] In one aspect of the invention, there is provided a bacterial composition comprising at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system fertilized with more than 9.1 kg of nitrogen per acre.

[0007] According to another aspect of the invention, a bacterial composition is provided that contains at least one bacterial strain bred to fix atmospheric nitrogen in an agricultural system that has been fertilized with more than 9.1 kg of nitrogen per acre.

[0008] According to a further aspect of the invention, a bacterial composition is provided that comprises at least one genetically engineered bacterial strain that fixes atmospheric nitrogen, wherein the at least one genetically engineered bacterial strain comprises exogenously added DNA, wherein said exogenously added DNA at least 80% identical with the corresponding native bacterial strain.

[0009] According to a further aspect of the invention, a seed composition is provided comprising the seeds of a plant inoculated with the bacterial composition.

[0010] Another aspect of the invention provides a method for growing a crop using a plurality of seeds with a seed composition inoculated with a bacterial composition.

[0011] In a further aspect of the invention, a method for applying a bacterial composition to a field is provided.

[0012] According to a further aspect of the invention, a fertilizer composition comprising a bacterial composition is provided.

[0013] According to another aspect of the invention, a method is provided for maintaining soil nitrogen levels. The method includes planting an agricultural crop inoculated with genetically engineered bacteria that fix atmospheric nitrogen into the soil of a field. The method also includes harvesting said crop, where between planting and harvesting, no more than 90% of the dose of nitrogen required to obtain said crop is applied to said soil of said field.

[0014] In a further aspect of the invention, a method for delivering a probiotic supplement to a crop is provided. The method includes coating the seeds of an agricultural crop with a seed coating, a seed treatment, or a seed dressing. Said seed coating, seed dressing, or seed dressing contains live representatives of said probiotic. In addition, the method includes introducing said crop seeds into the soil of the field.

[0015] In a further aspect of the invention, the engineered bacterial strain is a genetically engineered strain of a Gram-positive bacterium. In certain cases, a genetically engineered strain of gram-positive bacteria has an altered expression level of the Nif cluster regulator. In certain cases, a genetically engineered strain of Gram-positive bacteria expresses a reduced amount of the negative Nif cassette regulator. In certain cases, a genetically engineered bacterial strain expresses a reduced amount of GlnR . In certain cases, the genome of a genetically engineered bacterial strain encodes a polypeptide that is at least 75% identical to the sequence from the NifH database of the laboratory of J. Zehr (Zehr lab). In certain cases, the genome of a genetically engineered bacterial strain encodes a polypeptide that is at least 85% identical to the sequence from the NifH database of the laboratory of J. Zehr. In certain cases, the genome of a genetically engineered bacterial strain encodes a polypeptide that is at least 75% identical to the sequence from the NifH database of the laboratory of D. Buckley (Buckley lab). In certain cases, the genome of a genetically engineered bacterial strain encodes a polypeptide that is at least 85% identical to the sequence from the NifH database of D. Buckley's laboratory.

[0016] According to another aspect of the invention, a method is provided for increasing nitrogen fixation in a non-legume plant. The method includes applying to a plant a plurality of non-intergeneric bacteria, wherein said plurality includes non-intergeneric bacteria that (i) have an average colonizing capacity per unit of plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of tissue the root of the plant and (ii) produce a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour. In addition, many non-intergeneric bacteria, in planta , produce 1% or more of fixed nitrogen in the plant. In addition, each member of the plurality of non-intergeneric bacteria carries at least one genetic variation introduced into at least one gene or non-coding polynucleotide of the nitrogen fixation or assimilation genetic regulatory network such that the non-intergeneric bacteria become capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen.

[0017] In a further aspect of the invention, a method is provided for increasing nitrogen fixation in a non-legume plant. The method includes applying to a plant a plurality of non-intergeneric bacteria, wherein said plurality includes non-intergeneric bacteria that (i) have an average colonizing capacity per unit of plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of tissue the plant root and/or (ii) produce a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour. In addition, many non-intergeneric bacteria, in planta , produce 1% or more of fixed nitrogen in the plant. In addition, each member of the plurality of non-intergeneric bacteria carries at least one genetic variation introduced into at least one gene or non-coding polynucleotide of the nitrogen fixation or assimilation genetic regulatory network such that the non-intergeneric bacteria become capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen.

[0018] According to a further aspect of the invention, a method is provided for breeding strains of microorganisms to improve specific traits of agronomic importance. The method includes providing a plurality of strains of microorganisms having the ability to colonize the desired crop. The method also includes improving the regulatory networks affecting the trait through intragenomic rearrangement. In addition, the method includes evaluating microbial strains in a plurality of microbial strains to determine a measure of the trait. In addition, the method includes selecting from a plurality of microorganism strains one or more microorganism strains that provide an improvement in the trait in the case of the desired crop.

[0019] According to another aspect of the invention, a method is provided for breeding strains of microorganisms with the improvement of specific signs of agronomic importance. The method includes providing a plurality of strains of microorganisms having the ability to colonize the desired crop. The method also includes the introduction of genetic diversity in a variety of strains of microorganisms. In addition, the method includes evaluating microbial strains in a plurality of microbial strains to determine a measure of the trait. In addition, the method includes selecting from a plurality of microorganism strains one or more microorganism strains that provide an improvement in the trait in the case of the desired crop.

[0020] According to another aspect of the invention, a method is provided for increasing the amount of nitrogen available from the atmosphere in a non-legume plant. The method includes exposing said non-legume plant to genetically engineered non-intergeneric microorganisms, wherein said genetically engineered non-intergeneric microorganisms carry at least one genetic variation introduced into a nitrogen fixation genetic regulatory network, or at least one genetic variation introduced into genetic regulatory network for nitrogen assimilation.

[0021] According to a further aspect of the invention, a method is provided for increasing the amount of atmospheric nitrogen available to a corn plant. The method includes exposing said corn plant to genetically engineered non-intergeneric microorganisms carrying genetically engineered genetic variations in at least two genes selected from the group consisting of nifL , glnB , glnE and amtB .

[0022] According to another aspect of the invention, a method is provided for increasing the amount of atmospheric nitrogen available to a corn plant. The method includes exposing said corn plant to genetically engineered non-intergeneric microorganisms carrying at least one genetic variation introduced into a nitrogen fixation genetic regulatory network and at least one genetic variation introduced into a nitrogen assimilation genetic regulatory network, where said genetically engineered non-intergeneric microorganisms, in planta , produce in said corn plant at least 5% fixed nitrogen, as measured by ¹⁵ N dilution in crops grown in fields treated with a fertilizer containing 1.2% ¹⁵ N.

[0023] According to a further aspect of the invention, a method is provided for increasing nitrogen fixation in a non-legume plant. The method includes applying to a plant a plurality of non-intergeneric bacteria, wherein said plurality includes non-intergeneric bacteria that (i) have an average colonizing capacity per unit of plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of tissue the root of the plant and (ii) produce a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour. In addition, the product of (i) the average colonizing capacity per unit of plant root tissue and (ii) the fixed N produced per bacterial cell per hour is at least about 2.5 x 10 ^-8 mmol N per gram wet weight of plant root tissue per hour. . In addition, many non-intergeneric bacteria, in planta , produce 1% or more of fixed nitrogen in the plant. In addition, each member of the plurality of non-intergeneric bacteria carries at least one genetic variation introduced into at least one gene or non-coding polynucleotide of the nitrogen fixation or assimilation genetic regulatory network such that the non-intergeneric bacteria become capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen.

[0024] According to another aspect of the invention, a method is provided for increasing nitrogen fixation in a non-legume plant. The method includes applying a plurality of bacteria to a plant, wherein said plurality includes bacteria that (i) have an average colonizing capacity per unit of plant root tissue of at least about 1.0 × 10 ⁴ bacterial cells per gram wet weight of plant root tissue and/or ( ii) produce a fixed N in an amount of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour. In addition, many bacteria, in planta , produce 1% or more of fixed nitrogen in the plant.

[0025] In a further aspect of the invention, there is provided a non-intergeneric bacterial population capable of increasing nitrogen fixation in a non-legume plant, comprising a plurality of non-intergeneric bacteria that (i) have an average colonizing capacity per unit plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue and/or (ii) produce a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour. In addition, a plurality of non-intergeneric bacteria, in planta , produce in a plant grown in the presence of a plurality of non-intergeneric bacteria, 1% or more of fixed nitrogen. In addition, each member of the plurality of non-intergeneric bacteria carries at least one genetic variation introduced into at least one gene or non-coding polynucleotide of the nitrogen fixation or assimilation genetic regulatory network such that the non-intergeneric bacteria become capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen.

[0026] According to a further aspect of the invention, there is provided a bacterial population capable of increasing nitrogen fixation in a non-legume plant, wherein the bacterial population comprises a plurality of bacteria that (i) have an average colonizing capacity per unit of plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue; and/or (ii) produce a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour. In addition, many bacteria, in planta , produce 1% or more of fixed nitrogen in the plant.

[0027] In another aspect of the invention, bacteria are provided that (i) have an average colonizing capacity per unit plant root tissue of at least about 1.0 × 10 ⁴ bacterial cells per gram wet weight of plant root tissue and/or (ii) produce a fixed N in an amount of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour.

[0028] In a further aspect of the invention, non-intergeneric bacteria are provided that carry at least one genetic variation introduced into at least one gene or non-coding polynucleotide of the nitrogen fixation or assimilation genetic regulatory network such that the non-intergeneric bacteria are capable of atmospheric fixation. nitrogen in the presence of exogenous nitrogen, and wherein said bacteria (i) have an average colonizing capacity per unit plant root tissue of at least about 1.0 × 10 ⁴ bacterial cells per gram wet weight of plant root tissue and/or (ii) produce a fixed N in an amount of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour.

[0029] In a further aspect of the invention, a method of increasing nitrogen fixation in a plant is provided, comprising administering to the plant an effective amount of a composition that contains a purified population of bacteria that contains bacteria with a 16S nucleic acid sequence that is at least about 97% identical to the nucleic acid sequence selected from SEQ ID NOs: 85, 96, 111, 121, 122, 123, 124, 136, 149, 157, 167, 261, 262, 269, 277-283; a purified bacterial population that contains bacteria with a nucleic acid sequence that is at least about 90% identical to a nucleic acid sequence selected from SEQ ID NOs: 86-95, 97-110, 112-120, 125-135, 137-148 , 150-156, 158-166, 168-176, 263-268, 270-274, 275, 276, 284-295; and/or a purified bacterial population that contains bacteria with a nucleic acid sequence that is at least about 90% identical to a nucleic acid sequence selected from SEQ ID NOs: 177-260, 296-303; and wherein a plant administered with an effective amount of the composition exhibits an increase in nitrogen fixation compared to a plant without administration of the composition.

[0030] In a further aspect of the invention, isolated bacteria are provided comprising a 16S nucleic acid sequence that is at least about 97% identical to a nucleic acid sequence selected from SEQ ID NOs: 85, 96, 111, 121, 122, 123, 124, 136, 149, 157, 167, 261, 262, 269, 277-283; a nucleic acid sequence that is at least about 90% identical to a nucleic acid sequence selected from SEQ ID NOs: 86-95, 97-110, 112-120, 125-135, 137-148, 150-156, 158-166 , 168-176, 263-268, 270-274, 275, 276, 284-295; and/or a nucleic acid sequence that is at least about 90% identical to a nucleic acid sequence selected from SEQ ID NOs: 177-260, 296-303.

[0031] According to another aspect of the invention, a method for detecting a sequence with a non-natural compound is provided, comprising: amplifying a nucleotide sequence that is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77% , 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94 %, 95%, 96%, 97%, 98%, 99% or 100% identical to the sequence with SEQ ID NO: 372-405.

[0032] According to a further aspect of the invention, a method for detecting a sequence with a non-natural compound is provided, comprising: amplifying a nucleotide sequence that is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77% , 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94 %, 95%, 96%, 97%, 98%, 99% or 100% identical to the sequence of a fragment with at least 10 consecutive base pairs contained in SEQ ID NO: 372-405, where the specified fragment with consecutive base pairs consists of nucleotides at the intersection: located above the sequence containing SEQ ID NO: 304-337, and located below the sequence containing SEQ ID NO: 338-371.

[0033] In a further aspect of the invention, there is provided a sequence with a non-natural compound comprising a nucleotide sequence that is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95% , 96%, 97%, 98%, 99% or 100% identical to the sequence with SEQ ID NO: 372-405.

[0034] According to a further aspect of the invention, a sequence with a non-natural compound is provided, comprising a nucleotide sequence that is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95% , 96%, 97%, 98%, 99%, or 100% identical to the sequence of a fragment with at least 10 consecutive base pairs contained in SEQ ID NO: 372-405, where the specified fragment with consecutive base pairs consists of nucleotides at the intersection: the sequence above containing SEQ ID NOs: 304-337 and below the sequence containing SEQ ID NOs: 338-371.

[0035] In a further aspect of the invention, there is provided a bacterial composition comprising at least one reshaped bacterial strain that fixes atmospheric nitrogen, at least one remodeled bacterial strain containing exogenously added DNA, wherein said exogenously added DNA is at least 80% identical with the corresponding native bacterial strain.

[0036] According to a further aspect of the invention, a method is provided for maintaining soil nitrogen levels. The method includes planting a crop inoculated with reconstructed bacteria that fix atmospheric nitrogen into the soil of a field. The method also includes harvesting said crop, where between planting and harvesting, no more than 90% of the dose of nitrogen required to obtain said crop is applied to said soil of said field.

[0037] According to another aspect of the invention, a method for delivering a probiotic supplement to a crop is provided. The method includes coating seeds of a crop with a seed coating, seed treatment, or seed dressing, wherein said seed coating, seed dressing, or seed treatment contains live representatives of said probiotic. The method also includes applying said crop seeds to the field soil.

[0038] According to a further aspect of the invention, a method is provided for increasing the amount of nitrogen available from the atmosphere in a non-legume plant. The method includes exposing said non-legume plant to reshaped non-intergeneric microorganisms, wherein said remodeled non-intergeneric microorganisms carry at least one genetic variation introduced into the nitrogen fixation genetic regulatory network, or at least one genetic variation introduced into the assimilation genetic regulatory network. nitrogen.

[0039] According to a further aspect of the invention, a method is provided for increasing the amount of atmospheric nitrogen available to a corn plant. The method includes exposing said corn plant to reshaped non-intergeneric microorganisms carrying remodeled genetic variations in at least two genes selected from the group consisting of nifL , glnB , glnE and amtB .

[0040] According to another aspect of the invention, a method is provided for increasing the amount of atmospheric nitrogen available to a corn plant. The method includes exposing said corn plant to reconstituted non-intergeneric microorganisms carrying at least one genetic variation introduced into a nitrogen fixation genetic regulatory network and at least one genetic variation introduced into a nitrogen assimilation genetic regulatory network, wherein said reconstituted non- intergeneric microorganisms, in planta , produce at least 5% fixed nitrogen in said corn plant, as measured by ¹⁵ N dilution in crops grown in fields treated with a fertilizer containing 1.2% ¹⁵ N.

[0041] Additional aspects of the invention relate to a genus of microorganisms that has been evolved and optimized for nitrogen fixation in non-legume crops in planta . In particular, methods for increasing nitrogen fixation in a non-legume plant are described. The methods may include exposing the plant to a plurality of bacteria. Each member of the set carries one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacterial nitrogen fixation or assimilation genetic regulatory network such that the bacteria become capable of atmospheric nitrogen fixation in the presence of exogenous nitrogen. Bacteria are not intergeneric microorganisms. In addition, bacteria, in planta , produce 1% or more of fixed nitrogen in the plant.

[0042] Additional aspects of the invention relate to useful isolated microorganisms and compositions of microorganisms. In particular, isolated and biologically pure microorganisms are provided that are useful in increasing nitrogen fixation in crops, among other things. The described microorganisms can be used in their isolated and biologically pure states, as well as formulated in the composition. In addition, the description provides compositions of microorganisms containing at least two representatives of the described microorganisms, as well as methods of using these compositions of microorganisms.

INCLUDED AS A LINK

[0043] All publications, patents, and patent applications referenced herein are incorporated herein by reference to the same extent as if each particular publication, patent, or patent application were specifically and separately indicated for inclusion by reference.

BRIEF DESCRIPTION OF THE DRAWINGS

[0044] New features of the invention are described in detail in the attached claims. A better understanding of the features and advantages of the present invention may be obtained by referring to the following detailed description, which provides illustrative embodiments in which the basis of the invention is used, and to the accompanying figures, in which:

[0045] Figures 1A-B show the enrichment and isolation of nitrogen fixing bacteria. (A) Nfb agar plates were used to isolate single colonies of nitrogen fixing bacteria. (B) Semi-solid Nfb agar poured into a Balch tube. The arrow points to the pellicle of enriched nitrogen-fixing bacteria.

[0046] Figure 2 shows an exemplary screening of nifH by PCR. In this screen, positive bands were observed at ≈350 bp. for two colonies. The lower bands are primer dimers.

[0047] The figure 3 shows an example of screening by PCR of colonies after mutagenesis with the selection of CRISPR-Cas. CI006 colonies were screened with primers specific for the nifL locus. The expected wild-type PCR product is ≈2.2 kb, while the expected mutant is ≈1.1 kb. Seven out of ten screened colonies clearly showed the required deletion.

[0048] Figures 4A-D show the in vitro phenotypes of various strains. Acetylene recovery assay (ARA) activity of CI010 strain mutants ( Figure 4A ) and CI006 strain mutants ( Figure 4B ) grown in nitrogen fixation medium supplemented with 0 to 10 mM glutamine. The activity of the additional strains in ARA is shown in Figure 4C and the release profile of the two strains of ammonium over time is shown in Figure 4D .

[0049] Figure 5 shows the culture expression profile of 9 different genes in CI006 strains involved in diazotrophic nitrogen fixation. The numbers represent the amounts of each transcript. Various conditions are shown (0, 1, 10 mM glutamine and 0%, 10%, 20% atmospheric air in N ₂ ).

[0050] Figure 6 shows CI006 colonization of corn roots. Corn seedlings were inoculated with CI006 carrying the RFP expression plasmid. After two weeks of growth and maintenance of the plasmid by irrigating with the appropriate antibiotic, the roots were harvested and visualized by fluorescence microscopy. Colonization of the intercellular space of the roots can be observed.

[0051] Figure 7 shows nitrogen obtained at the microbial level for the wild-type strain (CI050) and the optimized strain (CM002).

[0052] Figures 8 show the Micro-Tom fruit weight test setup.

[0053] Figure 9 shows the Micro-Tom screening of 10 strains for plant fetal weight gain. The results of six repetitions are presented. For column 3, p=0.07. For column 7, p=0.05.

[0054] Figures 10A-C show additional results for the activity of candidate microorganisms and other candidate mutants grown in nitrogen fixation media supplemented with 0 to 10 mM glutamine in ARA.

[0055] Figure 11 shows a double mutant that exhibits higher ammonia excretion than the single mutant from which it is derived.

[0056] Figure 12 shows the NDFA obtained from the ¹⁵ N gas capture experiment (reverse extrapolated using day of exposure) to determine NDFA in corn plants under fertilization conditions.

[0057] Figure 13 shows the NDFA value obtained from the ¹⁵ N gas capture experiment (back extrapolated using day of exposure) to determine NDFA in Setaria plants under fertilization conditions.

[0058] Figure 14A shows the degree of ¹⁵ N gas incorporation. Plants inoculated with the evolved strain showed an increase in ¹⁵ N gas incorporation compared to non-inoculated plants.

[0059] Figure 14B shows 4 weeks after planting, up to 7% of the nitrogen in plants inoculated with the evolved strain comes from microbially fixed nitrogen.

[0060] Figure 14C shows the increase in leaf area (and other biomass measurements, data not shown) in plants inoculated with the evolved strain compared to uninoculated plants or inoculated wild type plants.

[0061] Figure 15A depicts evolved strains that show significantly higher production of nifH in root tissue as determined by in planta transcriptome testing.

[0062] Figure 15B shows that the level of fixed nitrogen detected in plant tissue correlates with the level of colonization of that particular plant by the HoME-optimized strain.

[0063] Figure 16A is a map of soil structures of various field soils tested for colonization. Soils, which initially were the source of a small number of microorganisms, are indicated by stars.

[0064] Figure 16B shows the colonization rate of strain 1 and strain 5 tested in four different soil types (circles). Both strains showed a relatively stable colonization profile in various soil types.

[0065] Figure 16C shows the colonization of strain 1 tested in field trials during the growing season. Strain 1 persisted in corn tissue up to 12 weeks after planting and began to show a decrease in colonization after that time.

[0040] Figure 17A is a diagram of microorganism breeding in embodiments.

[0041] Figure 17B is an expanded view of the microbiome composition as shown in Figure 17A .

[0042] Figure 17C shows the preparation of the root samples used in Example 7.

[0043] The figure 18 shows a line of modified strains derived from strain CI006.

[0044] The figure 19 shows a line of modified strains derived from strain CI019.

[0045] Figure 20 is a heat map of pounds of nitrogen delivered per acre/season by the microorganisms of the present invention, recorded as a function of microorganisms per gram wet weight per mmol nitrogen/microorganism/hour. Below the thin line across the larger image are microorganisms delivering less than one pound of nitrogen per acre/season, and above the line are microorganisms delivering more than one pound of nitrogen per acre/season. The table below the heatmap shows the exact mmol N produced by a microorganism per hour (mmol N/microorganism/hour) along with the exact CFU per gram wet weight (CFU/g wt) for each microorganism shown on the heatmap. The microorganisms used for the heat map were analyzed for N production in corn. For wild-type strains CI006 and CI019, maize root colonization data were obtained from one field plot. For the remaining strains, colonization was assumed to be the same as the wild type field level. N fixation activity was determined using an in vitro ARA assay with 5 mM glutamine.

[0046] The figure 21 shows the yield of plants under the influence of strain CI006. The area of the circles corresponds to the relative yield, while the shading corresponds to the specific MRTN. The x-axis represents the p value and the y-axis represents the probability of winning.

[0047] The figure 22 shows the yield of plants under the influence of strain CM029. The area of the circles corresponds to the relative yield, while the shading corresponds to the specific MRTN. The x-axis represents the p value and the y-axis represents the probability of winning.

[0048] The figure 23 shows the yield of plants under the influence of strain CM038. The area of the circles corresponds to the relative yield, while the shading corresponds to the specific MRTN. The x-axis represents the p value and the y-axis represents the probability of winning.

[0049] The figure 24 shows the yield of plants under the influence of strain CI019. The area of the circles corresponds to the relative yield, while the shading corresponds to the specific MRTN. The x-axis represents the p value and the y-axis represents the probability of winning.

[0050] The figure 25 shows the yield of plants under the influence of strain CM081. The area of the circles corresponds to the relative yield, while the shading corresponds to the specific MRTN. The x-axis represents the p value and the y-axis represents the probability of winning.

[0051] The figure 26 shows the yield of plants under the influence of strains CM029 and CM081. The area of the circles corresponds to the relative yield, while the shading corresponds to the specific MRTN. The x-axis represents the p value and the y-axis represents the probability of winning.

[0052] Figure 27 shows plant yields as cumulative gain/loss in bushels. The area of the circles corresponds to the relative yield, while the shading corresponds to the specific MRTN. The x-axis represents the p value and the y-axis represents the probability of winning.

[0053] Figures 28A-28E illustrate derived microorganisms that fix and release nitrogen in vitro under conditions similar to high nitrate agricultural soils. Figure 28A illustrates the regulatory network controlling nitrogen fixation and assimilation in PBC6.1, including the key nodes NifL, NifA, GS, GlnE depicted as a dual domain ATase-AR enzyme and AmtB. Figure 28B illustrates the genome of the PBC6.1 isolate Kosakonia sacchari . The three trajectories describing the genome correspond to PBC6.1, PBC6.38 transcription and strain differential expression data, respectively. Figure 28C illustrates the nitrogen fixation gene cluster and transcription data is enlarged to show finer details. Figure 28D illustrates nitrogenase activity at varying concentrations of exogenous nitrogen as determined by an acetylene reduction assay. The wild-type strain shows suppression of nitrogenase activity as glutamine concentrations increase, while derived strains show varying degrees of resistance. The error bars are the standard error of the mean of at least three biological replicates. Figure 28E illustrates the transient release of ammonia from derivative strains observed at mM concentrations. According to observations, wild-type strains do not release fixed nitrogen, and a small amount of ammonia accumulates in the media. The error bars are the standard error of the mean.

[0054] Figures 29A-29C illustrate greenhouse experiments demonstrating nitrogen fixation by microorganisms in corn. Figure 29A illustrates microorganism colonization six weeks after inoculation of corn plants with PBC6.1-derived strains. Error bars are the standard error of the mean of at least eight biological replicates. Figure 29B illustrates in planta transcription of nifH determined by isolation of total RNA from roots and subsequent Nanostring analysis. Only derived strains show nifH transcription under root conditions. Error bars are the standard error of the mean of at least 3 biological replicates. Figure 29C illustrates nitrogen fixation by microorganisms as determined by isotope tracer dilution in plant tissues. Derived microorganisms show a significant transfer of fixed nitrogen into the plant. The error bars are the standard error of the mean of at least ten biological replicates.

[0055] Figure 30 illustrates PBC6.1 colonization to nearly 21% of the root-associated microbiota in maize roots. Quantitative data based on sequencing of 16S amplicons from the rhizosphere and endosphere of maize plants inoculated with PBC6.1 and grown under greenhouse conditions.

[0056] Figure 31 illustrates transcriptional levels of nifA in PBC6.1-derived strains correlated with acetylene reduction levels. ARA analysis was performed as described in the methods section, after which a culture sample was taken and subjected to qPCR analysis to determine the levels of the nifA transcript. The error rates in each measurement are the standard error of the mean of at least three biological replicates.

[0057] Figure 32 illustrates the results of the summer 2017 field test. The yield results obtained demonstrate that the microorganisms of the invention can serve as a potential fertilizer substitute. For example, the use of the microorganism of the invention (ie, 6-403) resulted in higher yield than the wild type (WT) strain and higher yield than the untreated control (UTC). The "-25 lb N" treatment uses 25 lb N per acre less than standard farming practice in the region. The UTC treatment "100% N" is intended to refer to the standard agricultural practice in this region, where 100% of the standard N use is applied by the farmer. The microorganism "6-403" was deposited under NCMA ID 201708004 and can be found in Table A. It is a mutant of Kosakonia sacchari (also referred to as CM037) and is a wild-type mutant progeny strain of CI006.

[0058] Figure 33 illustrates the results of the summer 2017 test field experiment. The yield results obtained demonstrate that the microorganisms according to the invention act uniformly in different regions. In addition, the yield results demonstrate that the microorganisms of the invention perform well in both nitrogen deficient and nitrogen rich environments. The microorganism "6-881" (also known as CM094, PBC6.94) and being a mutant descendant of the wild-type strain Kosakonia sacchari CI006, is deposited under NCMA ID 201708002 and can be found in Table A. The microorganism "137-1034", which is a mutant descendant of the wild-type strain Klebsiella variicola CI137, has been deposited under NCMA ID 201712001 and can be found in Table A. The microorganism "137-1036", which is a mutant descendant of the wild-type strain Klebsiella variicola CI137, has been deposited under NCMA ID 201712002 and can be found in Table A. The microorganism "6-404" (also known as CM38, PBC6.38), and which is a mutant progeny of the wild-type strain Kosakonia sacchari CI006, is deposited under NCMA ID 201708003 and can be found in Table A. The "lack of nutrition" condition corresponds to conditions with 0% nitrogen. Conditions with "sufficient fertilizer content" corresponds to conditions with 100% nitrogen.

[0059] Figure 34 shows a line of modified strains derived from strain CI006 (also referred to as "6", wild type Kosakonia sacchari ).

[0060] Figure 35 shows a line of modified strains derived from strain CI019 (also referred to as "19", wild type Rahnella aquatilis ).

[0061] Figure 36 shows a line of modified strains derived from strain CI137 (also referred to as "137", wild type Klebsiella variicola ).

[0062] Figure 37 shows a line of modified strains derived from strain 1021 (wild type Kosakonia pseudosacchari ).

[0063] Figure 38 shows a line of modified strains derived from strain 910 (wild type Kluyvera intermedia ).

[0064] Figure 39 shows a line of modified strains derived from strain 63 (wild type Rahnella aquatilis ).

[0065] Figure 40 is a heat map of pounds of nitrogen delivered per acre/season by the microorganisms of the present invention, recorded as a function of microorganisms per gram wet weight per mmol nitrogen/microorganism/hour. Below the thin line across the larger image are microorganisms delivering less than one pound of nitrogen per acre/season, and above the line are microorganisms delivering more than one pound of nitrogen per acre/season. Table C in Example 12 lists the exact mmol N produced by a microorganism per hour (mmol N/microorganism/hr) along with the exact CFU per gram wet weight (CFU/g wt) for each microorganism shown on the heat map. . The data in Figure 40 is for microbial strains assessed for N production in corn in the field. Each point represents pounds N/acre produced by the microorganism using corn root colonization data from one field plot. N fixation activity was determined using an in vitro ARA assay with 5 mM N in the form of glutamine or ammonium phosphate.

[0066] Figure 41 is a heat map of pounds of nitrogen delivered per acre/season by the microorganisms of the present invention, recorded as a function of microorganisms per gram wet weight per mmol nitrogen/microorganism/hour. Below the thin line across the larger image are microorganisms delivering less than one pound of nitrogen per acre/season, and above the line are microorganisms delivering more than one pound of nitrogen per acre/season. Table D in Example 12 lists the exact mmol N produced by a microorganism per hour (mmol N/microorganism/hr) along with the exact CFU per gram wet weight (CFU/g wt) for each microorganism shown on the heat map. . The data in Figure 41 is obtained for strains of microorganisms evaluated for N production in corn under laboratory and greenhouse conditions. Each dot represents pounds N/acre produced by one strain. White dots represent strains for which corn root colonization data were obtained under greenhouse conditions. Black dots represent mutant strains for which corn root colonization levels are derived from average field corn root colonization levels of the wild-type parental strain. The shaded dots represent wild-type parental strains with their mean field levels of corn root colonization. In all cases, N fixation activity was determined by in vitro ARA assay with 5 mM N in the form of glutamine or ammonium phosphate.

DETAILED DESCRIPTION OF THE INVENTION

[0067] While various embodiments of the invention are presented and described herein, those skilled in the art will appreciate that such embodiments are provided by way of example only. Numerous variations, changes and substitutions can be made by those skilled in the art without departing from the invention. It should be understood that instead of the embodiments of the invention described in this document, you can use various alternatives.

[0068] The increased use of fertilizers is associated with environmental problems and is probably not possible for many economically stressed regions of the globe. In addition, many microbial industries focus on creating intergeneric micro-organisms. However, engineered microorganisms that are characterized/classified as intergeneric carry a heavy regulatory burden. These intergeneric microorganisms face not only a higher regulatory burden, making it difficult to spread and implement widely, but also more public scrutiny.

[0069] There are currently no engineered non-intergeneric microorganisms on the market that can enhance nitrogen fixation in non-legumes. This absence of such a microorganism represents the missing element in helping to create a truly environmentally friendly and more sustainable agricultural system in the 21st century.

[0070] The present invention solves the above problems and provides a non-intergeneric microorganism that has been engineered to readily fix nitrogen in crops. These micro-organisms are not characterized/classified as intergeneric micro-organisms and therefore will not face significant regulatory encumbrances. In addition, researched non-intergeneric microorganisms may help 21st century farmers become less dependent on the use of an ever-increasing amount of exogenous nitrogen fertilizers.

Definitions

[0071] The terms "polynucleotide", "nucleotide", "nucleotide sequence", "nucleic acid", and "oligonucleotide" are used interchangeably. They refer to the polymeric form of nucleotides of any length, which are deoxyribonucleotides or ribonucleotides or their analogues. Polynucleotides can have any three-dimensional structure and can perform any function, known or unknown. Non-limiting examples of polynucleotides are: coding or non-coding regions of a gene or gene fragment, loci (locus) determined by linkage analysis, exons, introns, messenger RNA (mRNA), transfer RNA (tRNA), ribosomal RNA (rRNA), small interfering RNA (siRNA), short hairpin RNA (shRNA), microRNA (miRNA), ribozymes, cDNA, recombinant polynucleotides, branched polynucleotides, plasmids, vectors, isolated DNA of any sequence, isolated RNA of any sequence, nucleic acid probes and primers. The polynucleotide may contain one or more modified nucleotides such as methylated nucleotides and nucleotide analogs. If present, modifications to the nucleotide structure may be provided before or after polymer assembly. Nucleotide sequences may be interrupted by non-nucleotide components. The polynucleotide can be further modified after polymerization, for example by conjugation with a labeled moiety.

[0072] "Hybridization" refers to a reaction in which one or more polynucleotides react to form a complex that is stabilized by hydrogen bonding between bases of nucleotide residues. Hydrogen bonding can occur by Watson-Crick base pairing, Hoogsteen bonding, or any other sequence-specific manner. The complex may contain two strands forming a duplex structure, three or more strands forming a multistrand complex, one self-hybridizing strand, or any combination of these. The hybridization reaction may be a step in a more complex process such as PCR initiation or enzymatic cleavage of a polynucleotide with an endonuclease. A second sequence that is complementary to the first sequence is referred to as a sequence "complementary" to the first sequence. The term "hybridizable" when applied to a polynucleotide refers to the ability of a polynucleotide to form a complex that is stabilized due to the formation of hydrogen bonds between bases of nucleotide residues in a hybridization reaction.

[0073] "Complementarity" refers to the ability of a nucleic acid to form hydrogen bond(s) with another nucleic acid sequence via conventional Watson-Crick or other non-traditional mating types. Complementarity percent refers to the percentage of residues in a nucleic acid molecule that can form hydrogen bonds (e.g., via Watson-Crick base pairing) with a second nucleic acid sequence (e.g., 5, 6, 7, 8, 9, 10 out of 10 are complementarity 50%, 60%, 70%, 80%, 90% and 100%, respectively). "Perfect complementarity" means that all consecutive residues of a nucleic acid sequence form hydrogen bonds with the same number of consecutive residues in a second nucleic acid sequence. As used herein, "substantially complementary" refers to a degree of complementarity that is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 97%, 98 %, 99% or 100% on areas 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 35, 40 , 45, 50 or more nucleotides, or refers to two nucleic acids that hybridize under stringent conditions. Sequence identity, e.g. for purposes of evaluating percent complementarity, can be determined by any suitable alignment algorithm, including but not limited to the Needleman-Wunsch algorithm (see e.g. EMBOSS Needle alignment tool available at www.ebi.ac.uk/Tools/psa /emboss_needle/nucleotide.html, optionally with default settings), BLAST algorithm (see for example the BLAST aligner available at blast.ncbi.nlm.nih.gov/Blast.cgi, optionally with default settings), or Smith's algorithm -Waterman (see for example the EMBOSS Water alignment tool available at www.ebi.ac.uk/Tools/psa/emboss_water/nucleotide.html, optionally with default settings). The optimal alignment can be estimated using any suitable parameters of the selected algorithm, including the default parameters.

[0074] Generally, "stringent hybridization conditions" refer to conditions under which a nucleic acid that is complementary to a target sequence preferentially hybridizes to the target sequence and does not substantially hybridize to non-target sequences. As a rule, stringent conditions are sequence dependent and vary depending on a number of factors. Generally, the longer a sequence is, the higher is the temperature at which the sequence specifically hybridizes to its target sequence. Non-limiting examples of stringent conditions are detailed in Tijssen (1993), Laboratory Techniques In Biochemistry And Molecular Biology-Hybridization With Nucleic Acid Probes Part I, Second Chapter "Overview of principles of hybridization and the strategy of nucleic acid probe assay", Elsevier, N.Y.

[0075] As used herein, "expression" refers to the process by which a polynucleotide is transcribed from a DNA template (e.g. into an mRNA or other RNA transcript) and/or the process by which the transcribed mRNA is then translated into peptides, polypeptides or squirrels. The transcripts and encoded polypeptides can be collectively referred to as a "gene product". If the polynucleotide is derived from genomic DNA, expression may involve mRNA splicing in a eukaryotic cell.

[0076] The terms "polypeptide", "peptide", and "protein" are used interchangeably herein to refer to polymers of amino acids of any length. The polymer may be linear or branched, it may contain modified amino acids, and it may be interrupted by non-amino acid regions. The terms also include a modified polymer of amino acids; for example, by disulfide bond formation, glycosylation, lipidation, acetylation, phosphorylation, or any other manipulation such as conjugation with a labeled moiety. As used herein, the term "amino acid" includes natural and/or non-natural or synthetic amino acids, including glycine and D or L optical isomers, and amino acid analogs and peptidomimetics.

[0077] As used herein, the term "about" is used as a synonym for the term "about". For illustration, the use of the term "about" in relation to the amount means that the values are slightly outside the specified values, for example, plus or minus from 0.1% to 10%.

[0078] The term "biologically pure culture" or "substantially pure culture" refers to a culture of the bacterial species described herein that does not contain other bacterial species in quantities sufficient to interfere with the replication of the culture or detectable by standard bacteriological methods.

[0079] "Plant productivity" generally refers to any measure of plant growth or development that is the reason the plant is grown. For food crops such as cereals or vegetables, "plant productivity" may refer to the yield of cereals or fruits harvested from a particular crop. As used herein, improved plant productivity refers broadly to improving the yield of grains, fruits, flowers or other plant parts harvested for various purposes, improving the growth of plant parts including stems, leaves and roots, promoting plant growth, maintaining a high chlorophyll content in leaves, increase in the number of fruits or seeds, increase in the weight of a single fruit or seed, decrease in NO ₂ emissions due to reduced use of nitrogen fertilizers, and similar improvements in plant growth and development.

[0080] Microorganisms in and around food crops can affect the traits of those crops. Plant traits that can be affected by microorganisms include: yield (eg, grain production, biomass production, fruit development, flower structure); nutrition (eg intake of nitrogen, phosphorus, potassium, iron, micronutrients); the ability to control abiotic stress (eg drought tolerance, salt tolerance, heat tolerance) and the ability to control biotic stress (eg pests, weeds, insects, fungi and bacteria). Strategies for modifying crop traits include: increasing concentrations of key metabolites; change in the temporal dynamics of the impact of microorganisms on key metabolites; linking the production/destruction of metabolites by microorganisms with new environmental parameters; reducing unwanted metabolites and improving the balance of metabolites or conditioning proteins.

[0081] As used herein, "regulatory sequence" refers to an operator, promoter, silencer, or terminator.

[0082] As used herein, " in planta " means in a plant, where the plant further includes parts, tissues, leaves, roots, stems, seeds, ovules, pollen, flowers, fruits of the plant, etc.

[0083] In certain embodiments, natural or endogenous regulatory sequences of the genes of the present invention are replaced with one or more intrageneric regulatory sequences.

[0084] As used herein, "administered" refers to administration through modern biotechnology, and not natural administration.

[0085] In certain embodiments, the implementation of the bacteria of the present invention is modified so that they are not natural bacteria.

[0086] In certain embodiments, the bacteria of the present invention are present in the plant in an amount of at least 10 ³ CFU, 10 ⁴ CFU, 10 ⁵ CFU, 10 ⁶ CFU, 10 ⁷ CFU, 10 ⁸ CFU, 10 ⁹ CFU, 10 ¹⁰ CFU , 10 ¹¹ CFU or 10 ¹² CFU per gram wet or dry weight of the plant. In certain embodiments, the bacteria of the present invention are present in the plant in an amount of at least about 10 ³ CFU, about 10 ⁴ CFU, about 10 ⁵ CFU, about 10 ⁶ CFU, about 10 ⁷ CFU, about 10 ⁸ CFU, about 10 ⁹ CFU , approximately 10 ¹⁰ CFU, approximately 10 ¹¹ CFU or approximately 10 ¹² CFU per gram of wet or dry weight of the plant. In certain embodiments, the bacteria of the present invention are present in the plant in an amount of at least 10 ³ to 10 ⁹ , 10 ³ to 10 ⁷ , 10 ³ to 10 ⁵ , 10 ⁵ to 10 ⁹ , 10 ⁵ to 10 ⁷ , from 10 ⁶ to 10 ¹⁰ , from 10 ⁶ to 10 ⁷ CFU per gram of wet or dry weight of the plant.

[0087] Fertilizers and exogenous nitrogen of the present invention may include the following nitrogen-containing molecules: ammonium, nitrate, nitrite, ammonia, glutamine, etc. The nitrogen sources of the present invention may include anhydrous ammonia, ammonium sulfate, urea, diammonium phosphate, formaldehyde urea, monoammonium phosphate, ammonium nitrate, liquid nitrogen fertilizers, calcium nitrate, potassium nitrate, sodium nitrate, and the like.

[0088] As used herein, "exogenous nitrogen" refers to non-atmospheric nitrogen readily available in the soil, field, or growing medium that is present under conditions without nitrogen restriction, including ammonia, ammonium, nitrate, nitrite, urea, uric acid, ammonic acids, etc.

[0089] As used herein, "non-limiting nitrogen conditions" refers to non-atmospheric nitrogen available in soil, field, environments at concentrations greater than about 4 mM nitrogen as described in Kant et al. (2010. J. Exp. Biol. 62(4):1499-1509), incorporated herein by reference.

[0090] As used herein, an "intergeneric microorganism" is a microorganism that is formed by an intentional combination of genetic material originally isolated from organisms of different taxonomic genera. Interchangeably with "intergeneric microorganism" you can use "intergeneric mutant". An exemplary "intergeneric microorganism" includes a microorganism containing a mobile genetic element that is first identified in a microorganism belonging to a genus other than the recipient microorganism. Additional explanation can be found at 40 C.F.R., among other things. 725.3.

[0091] In various aspects, the microorganisms explored herein are "non-intergeneric", which means that the microorganism organisms are not intergeneric.

[0092] As used herein, an "intrageneric microorganism" is a microorganism that is formed by an intentional combination of genetic material originally isolated from organisms of the same taxonomic genus. Interchangeably with "intrageneric microorganism" you can use "intrageneric mutant".

[0093] As used herein, "introduced genetic material" means genetic material that is added to the genome of the recipient and that remains in the genome of the recipient as a component.

[0094] In certain embodiments, the genetic regulatory network for nitrogen fixation and assimilation comprises polynucleotides encoding genes and non-coding sequences that direct, modulate, and/or regulate nitrogen fixation and/or assimilation by microorganisms, and may comprise nif cluster polynucleotide sequences (e.g., nif A, nif B, nif C, ... nifZ ), polynucleotides encoding nitrogen metabolism regulating protein C, polynucleotides encoding nitrogen metabolism regulating protein B, gln cluster polynucleotide sequences (e.g. gln A and glnD ), dra T and ammonium transporters/permeases . In certain cases, a Nif cluster may contain NifB , NifH , NifD , NifK , NifE , NifN , NifX , hesa , and NifV . In certain cases, a Nif cluster may contain a subset of NifB , NifH , NifD , NifK , NifE , NifN , NifX , hesa and NifV .

[0095] In certain embodiments, the fertilizer of the present invention contains at least 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16% , 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33 %, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66% , 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83 %, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% nitrogen by weight.

[0096] In certain embodiments, the fertilizer of the present invention contains at least about 5%, about 6%, about 7%, about 8%, about 9%, about 10%, about 11%, about 12%, about 13% , approximately 14%, approximately 15%, approximately 16%, approximately 17%, approximately 18%, approximately 19%, approximately 20%, approximately 21%, approximately 22%, approximately 23%, approximately 24%, approximately 25%, approximately 26%, approximately 27%, approximately 28%, approximately 29%, approximately 30%, approximately 31%, approximately 32%, approximately 33%, approximately 34%, approximately 35%, approximately 36%, approximately 37%, approximately 38% , approximately 39%, approximately 40%, approximately 41%, approximately 42%, approximately 43%, approximately 44%, approximately 45%, approximately 46%, approximately 47%, approximately 48%, approximately 49%, approximately 50%, approximately 51%, approximately 52%, approximately 53%, approximately 54%, approximately 55%, approximately 56%, approximately 57%, approximately 58%, approximately 59%, approximately 60%, approximately 61%, approximately 62%, approximately 63% , approximately 64%, approximately 65%, approximately 66%, approximately 67%, approximately 68%, approximately 69%, approximately 70%, approximately 71%, approximately 72%, approximately 73%, approximately 74%, approximately 75%, approximately 76%, approximately 77%, approximately 78%, approximately 79%, approximately 80%, approximately 81%, approximately 82%, approximately 83%, approximately 84%, approximately 85%, approximately 86%, approximately 87%, approximately 88% , about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, or about 99% nitrogen by weight.

[0097] In certain embodiments, the fertilizer of the present invention contains about 5% to 50%, about 5% to 75%, about 10% to 50%, about 10% to 75%, about 15% to 50% %, approximately 15% to 75%, approximately 20% to 50%, approximately 20% to 75%, approximately 25% to 50%, approximately 25% to 75%, approximately 30% to 50%, about 30% to 75%, about 35% to 50%, about 35% to 75%, about 40% to 50%, about 40% to 75%, about 45% to 50%, about 45% to 75% or about 50% to 75% nitrogen by weight.

[0098] In certain embodiments, the increase in nitrogen fixation and/or the production of 1% or more nitrogen in a plant is determined relative to control plants that have not been exposed to the bacteria of the present invention. All increases or decreases in bacteria are determined relative to control plants. All increases or decreases in plants are measured relative to control plants.

[0099] As used herein, a "constitutive promoter" is a promoter that is active under most conditions and/or during most stages of development. There are several advantages to using constitutive promoters in expression vectors used in biotechnology, for example: high production of proteins used to select transgenic cells or organisms; high expression of reporter proteins or selectable markers, allowing easy detection and quantification; a high level of transcription factor production, which is part of the transcription regulation system; production of compounds that require ubiquitous activity in the body; and the production of compounds that are essential at all stages of development. Non-limiting exemplary constitutive promoters include the CaMV 35S promoter, opine promoters, ubiquitin promoter, alcohol dehydrogenase promoter, and the like.

[0100] As used herein, a "non-constitutive promoter" is a promoter that is active under certain conditions, in certain cell types, and/or during certain stages of development. For example, tissue-specific, tissue-preferred, cell-type-specific, cell-type-preferred, inducible, and developmentally controlled promoters are non-constitutive promoters. Examples of developmentally controlled promoters include promoters that preferentially initiate transcription in certain tissues.

[0101] As used herein, an "inducible" or "repressible" promoter is a promoter that is under the control of chemicals or environmental factors. Examples of environmental conditions that can affect transcription by inducible promoters include anaerobic conditions, certain chemicals, presence of light, acidic or basic conditions, and so on.

[0102] As used herein, a "tissue-specific" promoter is one that initiates transcription only in certain tissues. Unlike constitutive gene expression, tissue-specific expression is the result of several interacting levels of gene regulation. Thus, in the art, it is sometimes preferable to use promoters from homologous or closely related species to achieve efficient and reliable expression of transgenes in specific tissues. This is one of the main reasons for the large number of tissue-specific promoters isolated from specific tissues found both in the scientific and patent literature.

[0103] As used herein, the term "operably linked" refers to the association of nucleic acid sequences in one nucleic acid fragment such that the function of one is controlled by the other. For example, a promoter is operably linked to a coding sequence when it can regulate the expression of that coding sequence (ie, that coding sequence is under the transcriptional control of the promoter). Coding sequences may be operably linked to regulatory sequences in either sense or antisense orientation. In another example, complementary RNA regions of the invention may be operably linked, directly or indirectly, 5' to the target mRNA, or 3' to the target mRNA, or to the target mRNA, or the first complementary region. is on the 5' end, and its complementary region is on the 3' end of the target mRNA.

[0104] In certain aspects, "applying a plurality of non-intergeneric bacteria to a plant" includes any means by which a plant (including parts of a plant such as a seed, root, stem, tissue, etc.) is brought into contact (i.e. , produce an effect) with these bacteria at any stage of the plant's life cycle. Thus, "applying a variety of non-intergeneric bacteria to a plant" includes any of the following means of exposing the plant (including plant parts such as seeds, root, stem, tissue, etc.) to said bacteria: spraying on the plant, dripping to a plant, application as a seed coat, application to a field that is then seeded, application to a field that has already been seeded, application to a field with mature plants, etc.

[0105] As used herein, "MRTN" is an abbreviation for maximum nitrogen return, and is used as an experimental indicator in the examples. The MRTN was developed at Iowa State University and information can be found at: http://cnrc.agron.iastate.edu/ The MRTN is the level of nitrogen at which the net economic return from nitrogen application is highest. The MRTN approach is a regional approach to developing state-specific recommendations for corn nitrogen levels. Nitrogen survey data was evaluated for Illinois, Iowa, Michigan, Minnesota, Ohio, and Wisconsin, where sufficient surveys were available for planting corn after soybeans and planting corn after corn. Studies were conducted using spring inter-row or split pre-plant/inter-row nitrogen application, and the plots were not irrigated except for the plots that are indicated for the irrigated sands in Wisconsin. The MRTN was developed at Iowa State University due to distinct differences in the way in which estimated nitrogen levels are required for corn production, incorrect assumptions about nitrogen recommendations, and concerns about application levels. By calculating the MRTN, practitioners can determine the following: (1) the nitrogen level at which the net economic return from applying nitrogen is maximum, (2) the economically optimal nitrogen level, which is the point where the last increase in nitrogen levels provides an increase in yield sufficient to pay additional nitrogen, (3) the value of corn kernel growth associated with the application of nitrogen, and the maximum yield, which is the yield at which the application of more nitrogen does not increase the yield of corn. Thus, MRTN calculations provide practitioners with the means to maximize corn plantings across regions while maximizing the financial benefit of nitrogen application.

[0106] The term mmol is an abbreviation for millimol, which is a thousandth (10 ^-3 ) of a mole, abbreviated as mole herein.

[0107] As used herein, the terms "microorganism" or "microbe" should be understood broadly. These terms, used interchangeably, include, by way of non-limiting examples, the two prokaryotic domains, bacteria and archaea. The term may also include eukaryotic fungi and protists.

[0108] The term "consortia of microorganisms" or "consortium of microorganisms" refers to a subset of the microbial community of specific microbial species or strains of species that can be described as having a common function, or can be described as participating in, or leading to, or correlated with a desired parameter, such as the phenotypic trait of interest.

[0109] The term "community of microorganisms" means a group of microorganisms containing two or more species or strains. Unlike consortia of microorganisms, a microbial community does not have to share a common function, or does not have to participate in, or lead to, or correlate with a desired parameter, such as a phenotypic trait of interest.

[0110] As used herein, "isolate", "isolated", "isolated microorganism" and similar terms are intended to mean that one or more microorganisms are separated from at least one of the substances with which they are associated in a particular environment. (eg soil, water, plant tissue, etc.). Thus, an "isolated microorganism" is not in its natural environment; rather, that by various methods described herein, the microorganism is removed from its natural state and placed in a non-naturally occurring state of existence. Thus, the isolated strain or isolated microorganism may be, for example, in the form of a biologically pure culture or in the form of spores (or other forms of the strain). In certain aspects, the isolated microorganism may be in association with an acceptable carrier, which may be an agriculturally acceptable carrier.

[0111] In certain aspects of the invention, isolated microorganisms exist as "isolated and biologically pure cultures". One skilled in the art should understand that an isolated and biologically pure culture of a particular microorganism means that said culture is substantially free of other living organisms and contains only the particular microorganism in question. The culture may contain varying concentrations of said microorganism. As noted herein, isolated and biologically pure microorganisms are often "not necessarily different from less pure or contaminated materials." See, e.g., In re Bergstrom, 427 F.2d 1394, (CCPA 1970) (for purified prostaglandins), see also In re Bergy, 596 F.2d 952 (CCPA 1979) (for purified microorganisms), see also, Parke-Davis & Co. v. H.K. Mulford & Co., 189 F. 95 (SDNY 1911) (Learned Hand describing purified adrenaline), partly affirmed, partly reviewed, 196 F. 496 (2d Cir. 1912), each of which is incorporated herein by reference . In addition, in certain aspects, the description provides for certain quantitative measures to limit the concentration or purity that must be detected in an isolated and biologically pure culture of microorganisms. The presence of these purity values in certain embodiments is an additional feature that distinguishes the microorganisms provided herein from those microorganisms that exist in a natural state. See, for example, Merck & Co. v. Olin Mathieson Chemical Corp., 253 F.2d 156 ( ^4th Cir. 1958) (which describes the purity limits for vitamin B12 produced by microorganisms), incorporated herein by reference.

[0112] As used herein, "individual isolates" should be understood as meaning a composition or culture containing predominant amounts of one genus, species or strain of microorganism after separation from one or more other microorganisms.

[0113] The microorganisms of the present invention may include spores and/or vegetative cells. In certain embodiments, the implementation of the microorganisms of the present invention include microorganisms in a viable but not cultivated (VBNC) state. As used herein, "spore" or "spores" refers to structures produced by bacteria and fungi that are adapted to survive and disperse. As a rule, spores are characterized as resting structures; however, spores are capable of differentiation during germination. Germination is the differentiation of spores into vegetative cells that are capable of metabolic activity, dew and reproduction. The germination of one spore leads to the formation of one fungal or bacterial vegetative cell. Fungal spores are units of asexual reproduction and, in certain cases, represent essential structures in the life cycles of fungi. Bacterial spores are structures for survival conditions that generally may not be conducive to the survival or growth of vegetative cells.

[0114] As used herein, "composition of microorganisms" refers to a composition containing one or more microorganisms of the present invention. In certain embodiments, the microbial composition is administered to plants (including various plant parts) and/or agricultural fields.

[0115] As used herein, "carrier", "acceptable carrier" or "agriculturally acceptable carrier" refers to a diluent, adjuvant, excipient or carrier with which a microorganism can be administered that does not adversely affect the microorganism.

Regulation of nitrogen fixation

[0116] In certain cases, the nitrogen fixation pathway can act as a target for genetic engineering and optimization. One of the traits that can be targeted by the methods described herein is nitrogen fixation. Nitrogen fertilizers are the biggest operating cost on farms and a major driver of higher yields in row crops such as corn and wheat. This document describes microbial waste products that can deliver renewable forms of nitrogen to non-legume crops. Although some endophytes have the genetic factors necessary for nitrogen fixation in pure crop, the main technical problem is that endophytes of wild-type cereals and grasses stop fixing nitrogen in fertilized fields. The use of chemical fertilizers and residual nitrogen levels in field soils signal the microorganism to turn off the biochemical pathway of nitrogen fixation.

[0117] For the development of a microorganism capable of fixing and transferring nitrogen to corn in the presence of a fertilizer, changes at the transcriptional and post-translational levels of components of the nitrogen fixation regulatory network may be beneficial. To this end, this document describes the microorganism host evolution (HoME) technology, which allows for the directed evolution of regulatory networks and obtaining new phenotypes. Also described herein are unique, proprietary libraries of nitrogen-fixing endophytes isolated from maize, together with extensive "omics" data on microbial-host interactions under various environmental conditions such as nitrogen deficiency and nitrogen excess. In certain embodiments, this technology enables the directed evolution of the endophyte genetic regulatory network towards producing microorganisms that actively fix nitrogen even in the presence of fertilizer in the field. Also described in this paper is an assessment of the industrial potential of derived microorganisms that colonize corn root tissues and produce nitrogen in fertilized plants, and an assessment of the compatibility of endophytes with standard formulation technologies and various soils to determine the possibility of integrating microorganisms into modern nitrogen control strategies.

[0118] To use elemental nitrogen (N) for chemical synthesis, living forms combine gaseous nitrogen (N ₂ ) available in the atmosphere with hydrogen in a process known as nitrogen fixation. Due to the energy-intensive nature of biological nitrogen fixation, diazotrophs (bacteria and archaea that fix atmospheric gaseous nitrogen) in the course of evolution have developed a developed and tight regulation of the nif gene cluster in response to environmental oxygen and available nitrogen. The Nif genes encode enzymes involved in nitrogen fixation (such as the nitrogenase complex) and proteins that regulate nitrogen fixation. Shamseldin (2013. Global J. Biotechnol. Biochem. 8(4):84-94) provides a detailed description of the nif genes and their products and is incorporated herein by reference. Methods for producing a trait-improved plant are described herein, including isolating bacteria from a first plant, introducing a genetic variation in the gene of the isolated bacteria to increase nitrogen fixation, exposing bacterial variants to a second plant, isolating bacteria from a second plant with an improved trait relative to the first plant, and repeating stages with bacteria isolated from the second plant.

[0119] In Proteobacteria, the regulation of nitrogen fixation is centered around the factor σ ⁵⁴ dependent enhancer-binding protein NifA, a positive transcriptional regulator of the nif cassette. Intracellular levels of active NifA control two key factors: transcription of the nifLA operon and inhibition of NifA activity by protein–protein interaction with NifL. Both of these processes respond to intracellular glutamine levels through a signaling cascade of PII proteins. This cascade is mediated by GlnD, which directly determines glutamine levels and catalyzes the uridylation or deuridylation of two PII regulatory proteins, GlnB and GlnK, in response to the absence or presence of bound glutamine, respectively. Under conditions of excess nitrogen, unmodified GlnB signals the deactivation of the nifLA promoter. However, under conditions of nitrogen restriction, post-translational modification of GlnB occurs, which inhibits its activity and leads to transcription of the nifLA operon. Thus, there is a tight control of nifLA transcription in response to environmental nitrogen through the signaling cascade of PII proteins. At the post-translational level of NifA regulation, GlnK inhibits the NifL/NifA interaction depending on the total level of free GlnK in the cell.

[0120] Transcription of NifA occurs from the operonnifLA, whose promoter activates phosphorylated NtrC, another σ-dependent⁵⁴ regulator. The NtrC phosphorylation state is mediated by NtrB histidine kinase, which interacts with deuridylated GlnB but not with uridylated GlnB. Under conditions of excess nitrogen, a high intracellular level of glutamine leads to deuridylation of GlnB, which then interacts with NtrB to deactivate its phosphorylase activity and activate its phosphatase activity, which leads to dephosphorylation of NtrC and deactivation of the promoter.nifLA. However, under conditions of nitrogen restriction, a low level of intracellular glutamine leads to uridylation of GlnB, which inhibits its interaction with NtrB and ensures NtrC phosphorylation and operon transcription.nifLA. Thus, there is a tight control of expressionnifLA in response to environmental nitrogen through signaling cascade of PII proteins. All ofnifA, ntrB, ntrC AndglnB are genes that can be mutated in the ways described herein. These processes can also respond to intracellular or extracellular levels of ammonia, urea, or nitrate.

[0121] There is also post-translational regulation of NifA activity in response to environmental nitrogen, typically through NifL-mediated inhibition of NifA activity. As a rule, the interaction between NifL and NifA is influenced by the signaling cascade of PII proteins through GlnK, although the nature of interactions between GlnK and NifL/NifA in diazotrophs varies considerably. In Klebsiella pneumoniae, both forms of GlnK inhibit the NifL/NifA interaction, and the interaction between GlnK and NifL/NifA is measured by the total level of free GlnK in the cell. Under conditions of excess nitrogen, deuridylated GlnK interacts with the ammonia transporter AmtB, which serves to block ammonia uptake by AmtB and sequester GlnK on the membrane, allowing NifA to be inhibited by NifL. On the other hand, in Azotobacter vinelandii , NifL/NifA interaction and NifA inhibition require interaction with deuridylated GlnK, while uridylation of GlnK inhibits this interaction with NifL. In diazotrophs lacking the nifL gene, there is evidence that inhibition of NifA activity occurs directly upon interaction with deuridylated forms of GlnK and GlnB under conditions of excess nitrogen. In some bacteria, the nif cluster may be under the regulatory influence of glnR, and additionally, in certain cases, this may include downregulation. Regardless of the mechanism, post-translational inhibition of NifA is an important regulator of the nif cluster in most known diazotrophs. Additionally, genes that can be mutated in the methods described herein are nifL, amtB , glnK , and glnR .

[0122] In addition to regulating the transcription of the nif gene cluster, many diazotrophs have evolved a mechanism for direct post-translational modification and inhibition of the nitrogenase enzyme itself, known as nitrogenase shutdown. It is mediated by ADP-ribosylation of the Fe protein (NifH) under conditions of excess nitrogen, which disrupts its interaction with the MoFe (NifDK) protein complex and eliminates nitrogenase activity. DraT catalyzes the ADP-ribosylation of the Fe protein and the shutdown of nitrogenase, while DraG catalyzes the removal of ADP-ribose and the reactivation of nitrogenase. As in the case of nifLA transcription and NifA inhibition, nitrogenase shutdown also regulates the signaling cascade of PII proteins. Under conditions of excess nitrogen, deuridylated GlnB interacts with DraT and activates it, while deuridylated GlnK interacts with DraG and AmtB to form a complex, sequestering DraG on the membrane. Under conditions of nitrogen restriction, the uridylated forms of GlnB and GlnK do not interact with DraT and DraG, respectively, leading to DraT inactivation and DraG diffusion to the Fe protein, where it removes ADP-ribose and activates nitrogenase. The methods described herein also provide for the introduction of genetic variations in the nifH, nifD, nifK and draT genes.

[0123] Although some endophytes have the ability to fix nitrogen in vitro , often these genetic factors are suppressed in the field by high levels of exogenous chemical fertilizers. To facilitate nitrogen fixation in the field, the determination of exogenous nitrogen levels and the expression of the nitrogenase enzyme can be separated. Improving the total nitrogenase activity over time further contributes to an increase in nitrogen production for crop use. Specific targets of genetic variation to facilitate field nitrogen fixation by the methods described herein include one or more genes selected from the group consisting of nifA, nifL, ntrB, ntrC, glnA, glnB, glnK, draT, amtB, gln D , glnE, nifJ, nifH, nifD, nifK, nifY, nifE, nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, and nifQ .

[0124] An additional target of genetic variation to facilitate nitrogen fixation in the field by the methods described herein is the NifA protein. As a rule, the NifA protein is an activator of the expression of nitrogen fixation genes. Increasing NifA production (either constitutively or under high ammonia conditions) overrides nature's way of determining ammonia levels. In addition, a decrease in the production of NifL proteins, a known NifA inhibitor, also leads to an increase in the level of free active NifA. In addition, an increase in the level of transcription of the nifAL operon (constitutively or under conditions of high ammonia content) also leads to an increase in the total level of NifA proteins. An increased level of nifAL expression is achieved by changing the promoter itself or by reducing the expression of NtrB (part of the ntrB and ntrC signaling cascade, which can initially lead to the shutdown of the nifAL operon under conditions of high nitrogen content). The high level of NifA achieved by these or any other methods described herein increases the activity of nitrogen fixation by endophytes.

[0125] Another target of genetic variation to facilitate nitrogen fixation in the field by the methods described herein is the GlnD/GlnB/GlnK PII signaling cascade. The intracellular level of glutamine determines the PII GlnD/GlnB/GlnK signaling cascade. GlnD active site mutations that abolish GlnD uridylate scavenging activity disrupt the nitrogen detection cascade. In addition, a decrease in the concentration of GlnB closes the cascade of determining the level of glutamine. These mutations "trick" the cells into detecting conditions of nitrogen restriction, thus increasing the level of nitrogen fixation activity. These processes can also respond to intracellular or extracellular levels of ammonia, urea, or nitrate.

[0126] Also targeted by genetic variation to facilitate nitrogen fixation in the field by the methods described herein is the amtB protein. The uptake of ammonia from the environment can be reduced by reducing the expression level of the amtB protein. Without intracellular ammonia, the endophyte is unable to detect high levels of ammonia, which prevents the downregulation of nitrogen fixation genes. Any ammonia entering the intracellular compartment is converted to glutamine. Intracellular glutamine levels are the primary means of determining nitrogen levels. A decrease in intracellular glutamine levels prevents cells from detecting high levels of ammonia in the environment. This can be done by increasing the expression level of glutaminase, the enzyme that converts glutamine to glutamate. In addition, the level of intracellular glutamine can also be reduced by reducing the level of glutamine synthetase (an enzyme that converts ammonia to glutamine). Diazotrophs rapidly assimilate fixed ammonia into glutamine and glutamate for use in cellular processes. Violation of ammonia assimilation can ensure the removal of fixed nitrogen for export from the cell in the form of ammonia. Fixed ammonia is predominantly assimilated to glutamine by glutamine synthetase (GS) encoded by glnA and then to glutamate by glutaminoxoglutarate aminotransferase (GOGAT). In certain examples, glnS encodes for glutamine synthetase. GS is post-translationally regulated by GS adenylyl transferase (GlnE), a bifunctional enzyme encoded by glnE that catalyzes adenylation and deadenylation of GS due to the activity of its adenylyl transferase (AT) and adenylyl retracting (AR) domains, respectively. Under conditions of nitrogen restriction, glnA is expressed and the AR domain of GlnE deadenylates GS, providing its activity. Under conditions of excess nitrogen, glnA expression is turned off and glutamine allosterically activates the AT domain of GlnE, which causes adenylation and deactivation of GS.

[0127] In addition, the draT gene can also be targeted by genetic variation to facilitate nitrogen fixation in the field by the methods described herein. After the cell produces nitrogen fixation enzymes, another level at which the cell negatively regulates fixation activity under high nitrogen conditions is nitrogenase shutdown. This switch-off can be eliminated by reducing the level of DraT expression.

[0128] Methods for imparting new phenotypes to microorganisms can be carried out at the transcriptional, translational, and post-translational levels. The transcriptional level includes changes in the promoter (such as a change in affinity for the sigma factor or transcription factor binding sites, including the deletion of all or part of the promoter) or a change in transcription terminators and attenuators. The translational level includes changes in ribosome binding sites and changes in mRNA degradation signals. The post-translational level includes mutations in the active sites of enzymes and changes in protein-protein interactions. These changes can be made in a variety of ways. Reducing the level of expression (or completely eliminating) can be done by replacing the natural ribosome binding site (RBS) or promoter with others with less strength/efficiency. The ATG start sites can be replaced with GTG, TTG or CTG start codons, resulting in a decrease in the translational activity of the coding region. Complete elimination of expression can be achieved by knockout (deletion) of the coding region of the gene. An open reading frame (ORF) shift is highly likely to result in a premature stop codon in the ORF, thus resulting in a non-functional truncated product. Similarly, in-frame insertion of stop codons also results in a non-functional truncated product. To reduce the effective concentration of a particular gene, the addition of a disruption tag to the N- or C-terminus can also be carried out.

[0129] Conversely, a certain level of expression of the genes described herein can be achieved using stronger promoters. To ensure high promoter activity under high nitrogen conditions (or any other conditions), a whole genome transcription profile under high nitrogen conditions can be obtained, and active promoters with the desired transcription level can be selected from this data set to replace the weak promoter. To improve translation initiation efficiency, weak start codons can be replaced with an ATG start codon. It is also possible to replace weak ribosome binding sites (RBS) with other RBSs with improved translation initiation efficiency. In addition, site-directed mutagenesis can also be performed to change the activity of the enzyme.

[0130] An increase in the level of nitrogen fixation that occurs in a plant can lead to a decrease in the amount of chemical fertilizers needed to produce crops and a decrease in greenhouse gas emissions (eg, monovalent nitric oxide).

Obtaining populations of bacteria

Isolation of bacteria

[0131] Microorganisms useful in the methods and compositions described herein can be obtained by isolating microorganisms from the surfaces or tissues of natural plants. Microorganisms can be obtained by grinding the seeds to isolate the microorganisms. Microorganisms can be obtained by planting seeds in various soil samples and isolating microorganisms from tissues. In addition, microorganisms can be obtained by inoculating plants with exogenous microorganisms and determining which microorganisms remain in plant tissues. Non-limiting examples of plant tissues may include seeds, shoots, leaves, sections, plant, bulbs, or tubers.

[0132] The method for obtaining microorganisms may be a method for isolating bacteria from soils. Bacteria can be collected from soils of various types. In a specific example, the soil can be characterized by such properties as high or low fertility, moisture levels, mineral levels, and various practical ways of growing plants. For example, the soil may be included in a crop rotation where different crops are planted in the same soil in successive planting seasons. Consistently growing different crops on the same soil can prevent disproportionate depletion of certain minerals. Bacteria can be isolated from plants growing in selected soils. Plant seedlings can be harvested at 2-6 weeks of growth. For example, at least 400 isolates can be collected per collection cycle. Soil and plant types reveal plant phenotypes, as well as the conditions that ensure the subsequent enrichment of certain phenotypes.

[0133] Microorganisms can be isolated from plant tissues to assess the characteristics of microorganisms. To isolate different types of associative microorganisms, such as rhizospheric bacteria, epiphytes or endophytes, the processing parameters of tissue samples can be varied. For enrichment with nitrogen-fixing bacteria, isolates can be cultured in nitrogen-free media. Alternatively, microorganisms can be obtained from worldwide strain banks.

[0134] to assess the characteristics of microorganisms, analyzes are carried out in the field. In certain embodiments, plant tissue can be processed for screening through high throughput processing of DNA and RNA. In addition, non-invasive measurements can be used to assess plant characteristics such as colonization. Measurements on wild-type microorganisms can be carried out in turn from plant to plant. Measurements on wild-type microorganisms can also be carried out in the field using medium throughput methods. Measurements can be taken sequentially over time. A model plant system can be used, including but not limited to Setaria .

[0135] Microorganisms in a plant system can be screened by transcriptional profiling of a microorganism in a plant system. Examples of screening by transcriptional profiling are the use of quantitative polymerase chain reaction (qPCR) methods, molecular barcodes for transcript detection, next generation sequencing, and labeling of microorganisms with fluorescent labels. Influencing factors can be measured to assess colonization in a greenhouse, including, but not limited to, microbiome, abiotic factors, soil conditions, oxygen, moisture, temperature, inoculation conditions, and root localization. Nitrogen fixation in bacteria can be assessed by determining gaseous ¹⁵ N/ ¹⁵ N in fertilizer (dilution) using IRMS or NanoSIMS as described herein. NanoSIMS is a high resolution secondary ion mass spectrometry. The NanoSIMS method is a way to study the chemical activity of biological samples. The reduction catalysis of the oxidation reactions that govern the metabolism of microorganisms can be investigated at the cellular, subcellular, molecular and elemental levels. NanoSIMS can provide high spatial resolution in excess of 0.1 µm. NanoSIMS can detect the use of tracers such as ¹³ C, ¹⁵ N and ¹⁸ O. Thus, NanoSIMS can be used to determine the reactivity of nitrogen in a cell.

[0136] Automated greenhouses can be used for plant analysis. Plant parameters in response to microbial attack include, but are not limited to, biomass determination, chloroplast analysis, CCD camera, volumetric tomography measurements.

[0137] One way to enrich a population of microorganisms is to match the genotype. For example, polymerase chain reaction (PCR) analysis with a directed primer or a specific primer. Primers designed for the nifH gene can be used to identify diazotrophs, since diazotrophs express the nifH gene during nitrogen fixation. Microbial populations can also be enriched through single-cell culture-independent and chemotaxis-based isolation approaches. Alternatively, targeted isolation of microorganisms can be carried out by culturing the microorganisms on selective media. For pre-planned approaches to enrich populations of microorganisms for desired traits, bioinformatics data can be guided and are described in this document.

Enrichment with microorganisms with the ability to fix nitrogen using bioinformatics

[0138] Bioinformatic methods can be used to identify and isolate plant growth promoting rhizobacteria (PGPR) that are selected based on their ability to effect nitrogen fixation. Microorganisms with a high capacity for nitrogen fixation can contribute to favorable traits in plants. Bioinformatics assays for identifying PGPR include, but are not limited to, genomics, metagenomics, targeted isolation, gene sequencing, transcriptome sequencing, and modeling.

[0139] Genomic analysis can be used to identify PGPR and confirm the presence of mutations by next generation sequencing methods, as described herein, and version control of microorganisms.

[0140] Metagenomics can be used to identify and isolate PGPR using a prediction algorithm for colonization. The metadata can also be used to identify the presence of the genetically engineered strain in environmental and greenhouse samples.

[0141] Transcriptome sequencing can be used to predict genotypes leading to PGPR phenotypes. In addition, transcriptome data is used to identify promoters for altering gene expression. Transcriptome data can be analyzed in combination with whole genome sequencing (WGS) to generate metabolic models and gene regulatory networks.

Culture of microorganisms

[0142] Microorganisms isolated from nature can be subjected to a culture process where the microorganisms are converted into a form that is genetically traceable and identifiable. One way to cultivate a microorganism is to construct antibiotic resistance properties in it. The process of constructing antibiotic resistance can begin with the determination of the antibiotic resistance of a strain of wild-type microorganisms. If the bacteria are sensitive to an antibiotic, then the antibiotic may be a good candidate for constructing antibiotic resistance. An antibiotic resistance gene or an antiselective suicide vector can then be introduced into the genome of the microorganism by recombinant methods. The anti-selective suicide vector may consist of a deletion of the gene of interest, a selectable marker, and the anti-selective marker sacBα . Counterselection can be used to replace the native DNA sequences of microorganisms with antibiotic resistance genes. For the simultaneous evaluation of several microorganisms, allowing parallel culture, the medium throughput method can be used. Alternative culture methods include the use of targeted nucleases to prevent looping of suicide vector sequences or to generate intermediate vector sequences.

[0143] DNA-containing vectors can be introduced into bacteria in various ways, including electroporation and chemical transformation. For transformation, you can use the standard vector library. An example of a gene editing method is CRISPR with prior testing of Cas9 to ensure Cas9 activity in microorganisms.

Non-transgenic engineering of microorganisms

[0144] A population of microorganisms with favorable properties can be obtained through directed evolution. Directed evolution is an approach where the process of natural selection is mimicked to evolve proteins or nucleic acids towards a user-defined goal. An example of directed evolution is when random mutations are introduced into a population of microorganisms, the microorganisms with the most favorable properties are selected, and the selected microorganisms are continued to grow. The most favorable properties in growth-promoting rhizobacteria (PGPRs) may be present in nitrogen fixation. The directed evolution method may be iterative and adaptive based on a selection process after each iteration.

[0145] Plant growth-promoting rhizobacteria (PGPR) with high nitrogen fixation capacity can be obtained. The evolution of PGPR can be carried out by introducing genetic variation. Genetic variation can be introduced by polymerase chain reaction mutagenesis, site-directed mutagenesis, saturation mutagenesis, fragment shuffling mutagenesis, homologous recombination, CRISPR/Cas9 systems, chemical mutagenesis, and combinations thereof. Through these approaches, random mutations can be introduced into a population of microorganisms. For example, mutants can be generated using synthetic DNA or RNA through site-directed mutagenesis. Mutants can be obtained using the means contained in the plasmids, which are later corrected. Genes of interest can be identified using other species libraries with improved properties, including but not limited to improved PGPR properties, improved cereal colonization, increased oxygen sensitivity, increased nitrogen fixation, and increased ammonia excretion. Intrageneric genes can be designed from these libraries using software such as Geneious or Platypus design software. Mutations can be engineered using machine learning. Mutations can be constructed using a metabolic model. Automated construction of mutations can be done using Platypus-like tools and will generate RNA for Cas-directed mutagenesis.

[0146] Intrageneric genes can be transferred to a host microorganism. In addition, reporter systems can also be transferred to the microorganism. Reporter systems characterize promoters, determine transformation success, screen for mutants, and act as negative selection agents.

[0147] Microorganisms carrying the mutation can be cultivated through serial passages. The colony of microorganisms contains one variant of the microorganism. Microbial colonies are screened using an automated colony picker and fluid handler. Mutants with a gene duplication and increased copy number express a higher genotype of the desired trait.

Selection of plant growth-promoting microorganisms based on nitrogen fixation

[0148] Microbial colonies can be screened using various nitrogen fixation assays. One way to determine nitrogen fixation is a single enzyme assay that measures nitrogen excretion. An alternative method is the acetylene recovery assay (ARA) with continuous sampling over time. ARA can be run in high-throughput microtube arrays. ARA can be performed on living plants and plant tissues. Media compositions and oxygen concentrations in media can be varied in ARA assays. Another method for screening variants of microorganisms is the use of biosensors. NanoSIMS and Raman microspectroscopy can be used to study the activity of microorganisms. In certain cases, bacteria can also be cultured and grown by fermentation methods in bioreactors. Bioreactors are designed to increase the resistance of bacterial growth and reduce the sensitivity of bacteria to oxygen. Microfermenter media based on high performance plates are used to assess oxygen sensitivity, nutrient requirements, nitrogen fixation and nitrogen excretion. Bacteria can also be co-cultured with competing or favorable microorganisms to determine hidden pathways. Flow cytometry can be used to screen for bacteria that produce high levels of nitrogen using chemical, colorimetric, or fluorescent indicators. Bacteria can be cultured in the presence or absence of a nitrogen source. For example, bacteria can be cultured with glutamine, ammonia, urea, or nitrates.

Removal of microorganisms

[0149] Breeding microorganisms is a way to systematically identify and improve the role of species in the crop microbiome. The method includes three steps: 1) selection of candidate species by mapping plant-microorganism interactions and predicting a regulatory network linked to a particular phenotype, 2) practical and predictable improvement in microorganism phenotypes by intraspecific crossing of regulatory networks and gene clusters, and 3) screening and selection of new genotypes of microorganisms that provide the required phenotypes of crops. For a systematic analysis of the improvement of strains, a model was obtained in which the dynamics of colonization of a community of microorganisms is linked to the genetic activity of key species. The model is used to predict the breeding of genetic targets and to increase the frequency of selection for improvements in microbiome-encoded properties of agricultural value. For a graphical representation of an embodiment of the process, see Figure 17A . Specifically, Figure 17A is a diagram of microorganism breeding according to embodiments. As illustrated in Figure 17A , rational crop microbiome improvement can be used to increase soil biodiversity, tune key species exposure, and/or alter the timing and expression of important metabolic pathways. To this end, the inventors have developed a microbial breeding pipeline to identify and improve the role of strains in the crop microbiome. The method includes three steps: 1) selection of candidate species by mapping plant-microorganism interactions and predicting a regulatory network linked to a particular phenotype, 2) practical and predictable improvement in microorganism phenotypes by intraspecific crossing of regulatory networks and gene clusters, and 3) screening and selection of new genotypes of microorganisms that provide the required phenotypes of crops. To systematically analyze the improvement of strains, the inventors use a model in which the dynamics of microbial community colonization are linked to the genetic activity of key species. This process provides a methodology for inferring and selecting improvements in microbiome-encoded properties of agronomic value.

[0150] Obtaining bacteria to improve plant traits (eg, nitrogen fixation) can be achieved through serial reseeding. The production of these bacteria, in addition to identifying bacteria and/or compositions capable of providing one or more improved traits in one or more plants, can be accomplished by selecting plants with a particular improved trait that is affected by the microbial flora. One way to obtain bacteria to improve a trait of a plant includes the steps of: (a) isolating the bacteria from the tissue or soil of the first plant; (b) introducing a genetic variation into one or more bacteria to produce one or more bacterial variants; (c) exposure of bacterial variants to a plurality of plants; (d) isolating the bacteria from the tissue or soil of one of the plurality of plants, wherein the plant from which the bacteria is isolated has an improved trait relative to other plants in the plurality of plants; and (e) repeating steps (b)-(d) with bacteria isolated from the trait-improved plant (step (d)). Steps (b)-(d) can be repeated any number of times (eg, once, twice, three times, four times, five times, ten times or more) until the improved plant trait reaches the desired level. Further, the plurality of plants may be more than two plants, such as 10 to 20 plants, or 20 or more, 50 or more, 100 or more, 300 or more, 500 or more, or 1000 or more plants.

[0151] In addition to obtaining a plant with an improved trait, a population of bacteria is obtained containing bacteria carrying one or more genetic variations introduced in one or more genes (for example, genes that regulate nitrogen fixation). By repeating the above steps, a bacterial population can be obtained that contains the most appropriate members of the population that correlates with the plant trait of interest. Bacteria in this population can be identified and their beneficial properties determined, for example, through genetic and/or phenotypic analysis. Genetic analysis can be performed on bacteria isolated in step (a). Phenotypic and/or genotypic information can be obtained using methods including: high-throughput screening of plant-derived chemical components, sequencing methods including high-throughput sequencing of genetic material, differential display methods (including DDOT-PCR and DD-PCR), nucleic acid microarray methods, RNA-sec (shotgun sequencing of the whole genome) and qPCR-RT (quantitative PCR with real-time detection). The information obtained can be used to obtain population profiling information about the identification characteristics and activity of the bacteria present, for example, phylogenetic analysis or microarray-based screening of nucleic acids encoding components of rRNA operons or other taxonomically informative loci. Examples of taxonomically informative loci include 16S rRNA gene, 23S rRNA gene, 5S rRNA gene, 5.8S rRNA gene, 12S rRNA gene, 18S rRNA gene, 28S rRNA gene, gyrB gene, rpoB gene, fusA gene, recA gene, coxl gene, nifD. Examples of taxonomic profiling methods for determining taxa present in a population are described in US 20140155283. Identification of bacteria may include characterizing the activity of one or more genes or one or more signal transduction pathways, for example genes associated with the nitrogen fixation pathway. also in bacterial populations there may be synergistic interactions (when two components, when combined, increase the desired effect more than additively) between different bacterial species.

Genetic variation - localization and sources of genome alterations

[0152] The genetic variation may be a gene selected from the group consisting of: nifA, nifL, ntrB, ntrC, glnA, glnB, glnK, draT, amtB, gln D, glnE, nifJ, nifH, nifD, nifK, nifY, nifE, nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, and nifQ . The genetic variation may be a variation in a gene encoding a protein with a functionality selected from the group consisting of: glutamine synthetase, glutaminase, glutamine synthetase adenylate transferase, transcription activator, transcription antiactivator, pyruvate:flavodoxin oxidoreductase, flavodoxin, or ADP-D-ribosyltransferase NAD ⁺ : dinitrogenase reductase. The genetic variation may be a mutation that results in one or more of: increased expression or activity of NifA or glutaminase; reduced expression or activity of NifL, NtrB, glutamine synthetase, GlnB, GlnK, DraT, AmtB; reduced GlnE adenylate scavenging activity; or reduced GlnD uridylate scavenging activity. The introduction of genetic variation may include the insertion and/or deletion of one or more nucleotides at the target site, for example, 1, 2, 3, 4, 5, 10, 25, 50, 100, 250, 500 or more nucleotides. A genetic variation introduced into one or more bacteria by the methods described herein may be a knockout mutation (e.g., promoter deletion, premature stop codon insertion or deletion, entire gene deletion), or it may be a deletion or elimination of protein domain activity (for example, a point mutation affecting the active site, or deletion of a portion of a gene encoding a significant portion of a protein product), or it may be a change or deletion of the regulatory sequence of the target gene. It is also possible to insert one or more regulatory sequences, including heterologous regulatory sequences and regulatory sequences found in the genome of the bacterial species or genus corresponding to the bacteria in which the genetic variation is being carried out. In addition, regulatory sequences can be selected based on the level of gene expression in a bacterial culture or plant tissue. The genetic variation may be a predetermined genetic variation that is specifically introduced into the target site. The genetic variation may be a random mutation in the target area. A genetic variation may be an insertion or deletion of one or more nucleotides. In some cases, many different genetic variations (eg, 2, 3, 4, 5, 10 or more) are introduced into one or more of the isolated bacteria before the bacteria are exposed to the plants to evaluate the improvement of a trait. The plurality of genetic variations may be any of the types described above, the same or different types, and any combination thereof. In some cases, a plurality of different genetic variations are performed sequentially, conducting the first genetic variation after the first isolation step, the second genetic variation after the second isolation step, and so on. so as to accumulate many genetic variations in bacteria gradually providing improved traits in associated plants.

Genetic variation - ways to introduce alterations in the genome

[0153] In general, the term "genetic variation" refers to any change introduced into a polynucleotide sequence relative to a reference polynucleotide, such as a reference genome or portion thereof, or a reference gene or portion thereof. A genetic variation may be referred to as a "mutation" and a sequence or organism containing the genetic variation may be referred to as a "genetic variant" or "mutant". Genetic variations can have a number of effects, such as an increase or decrease in a particular type of biological activity, the inclusion of gene expression, metabolism and cell signaling. Genetic variations can be specifically introduced into the target area or introduced randomly. A number of molecular biology tools and techniques are available for introducing genetic variation. For example, genetic variation can be introduced by polymerase chain reaction mutagenesis, site-directed mutagenesis, saturation mutagenesis, fragment shuffling mutagenesis, homologous recombination, recombination engineering, lambda red-mediated recombination, CRISPR/Cas9 systems, chemical mutagenesis, and combinations thereof. Chemical methods for introducing genetic variation include exposing DNA to a chemical mutagen, such as ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), N-nitrosourea (EN U), N-methyl-N-nitro-N'-nitrosoguanidine, -oxide, diethyl sulfate, benzopyrene, cyclophosphamide, bleomycin, triethylmelamine, monomeric acrylamide, nitrogen mustard, vincristine, diepoxyalkanes (e.g. diepoxybutane), ICR-170, formaldehyde, procarbazine hydrochloride, ethylene oxide, dimethylnitrosamine, 7,12 dimethylbenz(a)anthracene, chlorambucil, hexamethylphosphamide, bisulfan, and the like. Mutation-inducing agents by irradiation include ultraviolet irradiation, γ-irradiation, x-rays, and fast neutron bombardment. Also, genetic variations can be introduced into nucleic acids, for example, using trimethylpsoralen with ultraviolet irradiation. Another suitable method for generating genetic variation is the random or targeted insertion of a transposable DNA element, such as a transposon. Genetic variations can be introduced into the nucleic acid by amplification in an in vitro cell-free system, for example, using a polymerase chain reaction (PCR) method, such as reduced fidelity PCR. Genetic variations can be introduced into nucleic acids in vitro using DNA shuffling techniques (eg, exon shuffling, domain swaps, and the like). Genetic variations can also be introduced into nucleic acids as a result of a lack of DNA repair enzymes in the cell, for example, it is believed that the presence in the cell of a mutant gene encoding a mutant DNA repair enzyme will result in a high mutation rate (i.e., from about 1 mutation /100 genes to 1 mutation/10,000 genes) in the cell genome. Non-limiting examples of genes encoding DNA repair enzymes include Mut H, Mut S, Mut L, and Mut U and their homologues in other species (e.g., MSH1 to MSH6, PMS1 to PMS2, MLH1, GTBP, ERCC-1 and so on.). Examples of descriptions of various methods for introducing genetic variation are given, for example, in Stemple (2004) Nature 5:1-7; Chiang et al. (1993) PCR Methods Appl 2(3): 210-217; Stemmer (1994) Proc. Natl. Acad. sci. USA 91:10747-10751 and US Pat. Nos. 6,033,861 and 6,773,900.

[0154] Genetic variations introduced into microorganisms can be classified as transgenic, cisgenic, intragenomic, intrageneric, intergeneric, synthetic, evolved, permuted, or SNP.

[0155] To provide improvements to the properties described above, genetic variation can be introduced into a variety of metabolic pathways in microorganisms. Typical pathways include sulfur uptake pathways, glycogen biosynthesis, glutamine regulation pathway, molybdenum uptake pathway, nitrogen fixation pathway, ammonia assimilation, ammonia excretion or secretion, nitrogen uptake, glutamine biosynthesis, anaerobic ammonium oxidation, phosphate dissolution, organic acid transport, organic acid production , agglutitin production, free reactive oxygen radical scavenging genes, indoleacetic acid biosynthesis, trehalose biosynthesis, enzymes or plant cell wall disruption pathways, root attachment genes, exopolysaccharide secretion, glutamate synthase synthesis pathway, iron uptake pathways, siderophore pathways, chitinase pathway, ACC deaminase , glutathione biosynthesis, phosphate signaling genes, quenching pathway, cytochrome pathways, hemoglobin pathway, bacterial hemoglobin-like protein pathway, rsmZ small RNAs, rhizobiotoxin biosynthesis, lapA adhesion protein, AHL quorum sensing pathway, phenazine biosynthesis, cyclic lipopeptide biosynthesis and antibiotic production.

[0156] CRISPR/Cas9 (regularly spaced short palindromic repeats)/CRISPR-associated (Cas) systems can be used to introduce the desired mutations. CRISPR/Cas9 provides bacteria and archaea with acquired immunity against viruses and plasmids through the use of CRISPR RNA (csRNA) to direct the silencing of invading nucleic acids. The Cas9 protein (or its functional equivalent and/or variant, i.e., a Cas9-like protein) naturally has DNA endonuclease activity that depends on the association of the protein with two natural or synthetic RNA molecules, called cpRNA and transcpRNA (also called guide RNA). In some cases, two molecules are covalently linked to form a single molecule (also called a single guide RNA ("sRNA"). Thus, a Cas9 protein or a Cas9-like protein associates with a DNA-directed RNA (where this term includes a two-guide RNA configuration). and single guide RNA configuration) that activates a Cas9 or Cas9-like protein and directs the protein to a target nucleic acid sequence. If Cas9 or a Cas9-like protein retains its enzymatic function, it cleaves the target DNA to form a double-strand break, which can to modify the genome (i.e., editing: deletions, insertions (when a donor polynucleotide is present), substitution, etc.), thereby altering gene expression. Certain variants of Cas9 (which are included in the term Cas9-like) alter so so that they have reduced DNA cleavage activity (in some cases they cleave one strand instead of both strands of the target DNA, while in other cases their activity is severely reduced to no DNA cleavage activity). Additional illustrative descriptions of CRISPR systems for introducing genetic variation can be found, for example, in US 8795965.

[0157] As a method of cyclic amplification in polymerase chain reaction (PCR) mutagenesis, mutagenic primers are used to introduce desired mutations. PCR is carried out in the form of cycles of denaturation, annealing and completion. After amplification by PCR, selection of mutant DNA and removal of the original plasmid DNA can be carried out by: 1) replacing dCTP during PCR with hydroxymethylated dCTP, followed by restriction enzyme digestion to remove only the non-hydroxymethylated original DNA; 2) simultaneous mutagenesis of the antibiotic resistance gene and the gene under study, changing the antibiotic resistance of the plasmid to resistance to other antibiotics, where resistance to new antibiotics then facilitates the selection of the desired mutation; 3) cleavage of the original methylated template DNA after introducing the desired mutation with the restriction enzyme DpnI, which cleaves only methylated DNA, whereby the mutant unmethylated strands are isolated; or 4) obtaining circular molecules of mutant PCR products in an additional ligation reaction with an increase in the efficiency of transformation of the mutant DNA. Further description of illustrative methods can be found, for example, in US 7132265, US 6713285, US 6673610, US 6391548, US 5789166, US 5780270, US 5354670, US 5071743 and US 20100267147.

[0158] Oligonucleotide-specific mutagenesis, also referred to as site-specific mutagenesis, typically uses a synthetic DNA primer. This synthetic primer contains the desired mutation and is complementary to the template DNA around the site of the mutation so that it can hybridize to the DNA in the gene of interest. The mutation may be a single nucleotide substitution (point mutation), a multi-nucleotide substitution, a deletion or insertion, or a combination thereof. The single strand primer is then completed using a DNA polymerase that copies the rest of the gene. The gene copied in this manner contains a mutated region and can then be introduced into a host cell as a vector and cloned. Finally, mutants can be selected by DNA sequencing to verify that they contain the desired mutation.

[0159] Genetic variations can be introduced using PCR with reduced accuracy. In this method, a gene of interest is amplified using a DNA polymerase under conditions of insufficient sequence replication fidelity. The result is that the amplification products contain at least one sequence error. When a gene is amplified and the resulting reaction product(s) contains one or more sequence changes from the template molecule, the resulting products are mutated from the template. Another way to introduce random mutations is to expose the cells to a chemical mutagen such as nitrosoguanidine or ethylmethanesulfonate (Nestmann, Mutat Res 1975 June; 28(3):323-30) and then isolate the vector containing the gene from the host.

[0160] Another method of random mutagenesis is saturation mutagenesis, which tries to get all or almost all possible mutations in a particular region or small region of a gene. In a broad sense, saturation mutagenesis consists of mutagenesis of a complete set of mutagenic cassettes (where each cassette is, for example, 1-500 bases long) in a specific mutagenesis polynucleotide sequence (where the mutagenesis sequence is, for example, 15 to 100,000 bases long). Thus, a group of mutations (eg, ranging from 1 to 100 mutations) is introduced into each cassette to be mutated. The group of mutations introduced into one cassette may be different or the same as the second group of mutations introduced into the second cassette during one round of saturation mutagenesis. Examples of such groups are deletions, additions, specific codon groups, and specific nucleotide cassette groups.

[0161] Fragment shuffling mutagenesis, also referred to as DNA shuffling, is a way to rapidly reproduce favorable mutations. In an exemplary shuffling method, DNase is used to fragment a set of original genes into pieces of length, eg, approximately 50-100 bp. After that, a polymerase chain reaction (PCR) is carried out without primers - DNA fragments with sufficient overlap of homologous sequences are annealed to each other and then they are completed by DNA polymerase. After some DNA molecules reach the size of the original genes, several cycles of this completion are carried out by PCR. These genes can then be amplified by another PCR, this time with the addition of primers designed to be complementary to the ends of the chains. The primers may have additional sequences added to their 5' ends, such as restriction enzyme recognition sequences required for ligation into a cloning vector. Additional examples of permutation methods are given in US 20050266541.

[0162] Mutagenesis by homologous recombination involves the recombination of an exogenous DNA fragment and a target polynucleotide sequence. After the formation of double-strand breaks, the sections of DNA at the 5' ends of the breaks are removed in a process called resection. In a subsequent strand insertion step, the 3' protruding end of the broken DNA molecule is then "introduced" into a similar or identical DNA molecule that has not been broken. The method can be used to remove genes, remove exons, add genes, and introduce point mutations. Mutagenesis by homologous recombination may be permanent or conditional. Typically, a recombination matrix is also provided. The recombination template may be a component of another vector contained in a separate vector or provided as a separate polynucleotide. In certain embodiments, the recombination template is designed to serve as a template in homologous recombination, for example, within or adjacent to the target sequence being cut or cleaved by the site-specific nuclease. The length of the template polynucleotide may be any suitable length, for example, the length may be about or more than about 10, 15, 20, 25, 50, 75, 100, 150, 200, 500, 1000 or more nucleotides. In certain embodiments, the template polynucleotide is complementary to the portion of the polynucleotide containing the target sequence. When optimally aligned, the template polynucleotide may overlap with one or more nucleotides of the target sequence (e.g., about or more than about 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100 or more nucleotides). In certain embodiments, when the template sequence and the polynucleotide containing the target sequence are optimally aligned, the closest nucleotide of the template polynucleotide is within about 1, 5, 10, 15, 20, 25, 50, 75, 100, 200, 300, 400 , 500, 1000, 5000, 10000 or more nucleotides from the target sequence. Non-limiting examples of site-specific nucleases useful in homologous recombination methods include zinc finger nucleases, CRISPR nucleases, TALE nucleases, and meganuclease. For further description of the use of such nucleases, see, for example, US 8795965 and US 20140301990.

[0163] To obtain genetic variations, mutagens can be used, by which point mutations and short deletions, insertions, transversions and/or transitions are predominantly obtained, including chemical mutagens or irradiation. As non-limiting examples, mutagens include ethyl methanesulfonate, methyl methanesulfonate, N-ethyl-N-nitrosourea, triethylmelamine, N-methyl-N-nitrosourea, procarbazine, chlorambucil, cyclophosphamide, diethyl sulfate, monomeric acrylamide, melphalan, nitrogen mustard, vincristine, dimethylnitrosamine, N- methyl-N'-nitronitrosoguanidine, nitrosoguanidine, 2-aminopurine, 7,12-dimethylbenz(a)anthracene, ethylene oxide, hexamethylphosphamide, bisulfan, diepoxyalkanes (diepoxyoctane, diepoxybutane, etc.), 2-methoxy-6-chloro-dihydrochloride 9[3-(ethyl-2-chloroethyl)aminopropylamino]acridine and formaldehyde.

[0164] The introduction of genetic variation may be a work in progress such that certain bacteria in the treated bacterial population carry the desired mutation while others do not. In certain cases, selection pressure must be applied to enrich for bacteria carrying the desired genetic variation. Generally, the selection of successful genetic variants involves selection for or against a particular functionality conferred or eliminated by the genetic variation, such as insertion of an antibiotic resistance gene or removal of a metabolic activity allowing the conversion of a non-lethal compound to a lethal metabolite. Also, selection pressure can be applied on the basis of the polynucleotide sequence itself, such that only the desired genetic variation needs to be introduced (eg also without the need for a selectable marker). In this case, the selection pressure may include segregation of genomes with no genetic variation introduced into the target region, such that selection is effectively directed against the reference sequence into which the genetic variation is to be introduced. Typically, cleavage occurs within 100 nucleotides of the target site (eg, within 75, 50, 25, 10 or less nucleotides of the target site, including cleavage at or within the target site). Cleavage can be directed by a site-specific nuclease selected from the group consisting of zinc finger nuclease, CRISPR nuclease, TALE nuclease (TALEN), or meganuclease. Such a process is similar to the processes for increasing homologous recombination at the target site, except that no template for homologous recombination is provided. As a result, bacteria without the required genetic variation are more likely to undergo cleavage, which in the absence of repair leads to cell death. The bacteria surviving the selection can then be isolated for use in plant treatment to evaluate the improved trait imparted.

[0165] CRISPR nuclease can be used to direct the cleavage at the target site. By using Cas9 to kill wild-type cells, improved selection of mutant microorganisms can be achieved. The plants are then inoculated with mutant microorganisms to confirm the symbiosis and provide evolutionary pressure to select effective symbionts. The microorganisms can then be re-isolated from plant tissues. In systems with CRISPR nucleases used to select for the absence of variants, elements similar to those described above regarding the introduction of genetic variation elements can be used, except that they do not provide a template for homologous recombination. Thus, cleavage directed to the target site increases the death of exposed cells.

[0166] Other variants of specifically induced cleavage at the target site are available, such as zinc finger nucleases, TALE nuclease (TALEN) systems, and meganuclease. Zinc finger nucleases (ZFNs) are artificial DNA endonucleases obtained by fusing the DNA-binding domain of zinc fingers with a DNA cleavage domain. ZFNs can be designed to cleave desired DNA sequences and this allows zinc finger nucleases to cleave unique target sequences. When introduced into a cell, ZFN can be used to edit a target DNA in the cell (eg, the cell's genome) by inducing double-strand breaks. Transcription activator-like effector nucleases (TALEN) are artificial DNA endonucleases obtained by fusion of the TAL effector DNA-binding domain (like transcription activators) with a DNA cleavage domain. TALEN can be quickly designed to bind virtually any desired DNA sequence, and when introduced into a cell, TALEN can be used to edit a target DNA in a cell (eg, the cell's genome) by introducing double-strand breaks. Meganucleases (homing endonuclease) are endodeoxyribonucleases characterized by a large recognition site (double-stranded DNA sequences from 12 to 40 base pairs in length). Meganucleases can be used to replace, delete or modify sequences in a narrow manner. By modifying the sequences they recognize through protein engineering, the target sequence can be changed. Meganucleases can be used to modify all types of genomes, bacteria, plants or animals, and are generally grouped into four families: the LAGLIDADG family (SEQ ID NO:1) , the GIY-YIG family, the His-Cyst box family, and the HNH family. Exemplary homing endonucleases include I-SceI, I-CeuI, PI-PspI, PI-Sce, I-SceIV, I-CsmI, I-PanI, I-SceII, I-PpoI, I-SceIII, I-CreI, I -TevI, I-TevII and I-TevIII.

Genetic Variation - Methods of Identification

[0167] The microorganisms of the present invention can be identified by one or more genetic modifications or alterations that are made in the specified microorganism. One way in which said genetic modification or alteration can be identified is by comparison with SEQ ID NO, which contains a portion of the microorganism's genomic sequence sufficient to identify the genetic modification or alteration.

[0168] In addition, in the case of microorganisms that should not carry genetic modifications or alterations (eg, wild type) introduced into their genomes, 16S nucleic acid sequences can be used as described to identify these microorganisms. The 16S nucleic acid sequence is an example of a "molecular marker" or "genetic marker" which is an indicator that is used in methods for visualizing differences in characteristics of nucleic acid sequences. Examples of other such indicators are restriction fragment length polymorphism (RFLP) marks, amplified fragment length polymorphism (AFLP) marks, single nucleotide polymorphisms (SNPs), insertion mutations, microsatellite marks (SSR), sequence-characterized amplified regions (SCAR), amplified restriction polymorphism marks sequences (CAPS) or isoenzyme labels or combinations of labels described in this section that define a particular genetic and chromosomal position. The tags further include polynucleotide sequences encoding 16S or 18S rRNA and internal transcribed spacer (ITS) sequences, which are sequences between the small subunit and large subunit rRNA genes, which have proven to be particularly useful in determining similarities or differences when compared to each other. . In addition, unique sequences found in genes of interest (eg, nif H,D,K,L,A, glnE, amtB, etc.) are used as described to identify the microorganisms described herein.

[0169] The primary structure of the 16S rRNA large subunit contains a specific combination of conserved, variable, and hypervariable regions that evolve at different rates and allow discrimination between very ancient lineages, such as domains, and more recent lineages, such as genera. The secondary structure of the 16S subunit contains approximately 50 helices, resulting in base pairing of approximately 67% of the residues. These highly conserved secondary structural features are of great functional importance and can be used to provide positional homology in multiple sequence alignments and phylogenetic analyses. Over the past few decades, the 16S rRNA gene has become the most sequenced taxonomic marker, and it is the cornerstone of the modern systematic classification of bacteria and archaea. (Yarza et al. 2014. Nature Rev. Micro. 12:635-45).

[0170] Thus, In certain aspects, the description provides a sequence that is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96% , 97%, 98%, 99%, or 100% identical in sequence to any sequence in tables E , F , G , or H.

[0171] Thus, In certain aspects, the description provides a microorganism that contains a sequence that is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78% , 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95 %, 96%, 97%, 98%, 99% or 100% sequence identical to SEQ ID NO: 62-303. These sequences and their associated descriptions can be found in Tables F , G , and H.

[0172] In certain aspects, the description provides a microorganism that contains a 16S nucleic acid sequence that is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78% , 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95 %, 96%, 97%, 98%, 99% or 100% identical in sequence to SEQ ID NOs: 85, 96, 111, 121, 122, 123, 124, 136, 149, 157, 167, 261, 262, 269, 277-283. These sequences and their associated descriptions can be found in Tables G and H.

[0173] In certain aspects, the description provides a microorganism that contains a nucleic acid sequence that is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95% , 96%, 97%, 98%, 99%, or 100% identical in sequence to SEQ ID NOs: 86-95, 97-110, 112-120, 125-135, 137-148, 150-156, 158-166 , 168-176, 263-268, 270-274, 275, 276, 284-295. These sequences and their associated descriptions can be found in Tables G and H.

[0174] In certain aspects, the description provides a microorganism that contains a nucleic acid sequence that is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95% , 96%, 97%, 98%, 99%, or 100% sequence identical to SEQ ID NOs: 177-260, 296-303. These sequences and their associated descriptions can be found in Tables G and H.

[0175] In certain aspects, the description provides a microorganism, or a primer, or a probe, or a sequence with a non-natural compound that contains, a nucleic acid sequence that is at least about 70%, 71%, 72%, 73%, 74% , 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91 %, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% sequence identical to SEQ ID NO: 304-424. These sequences and their associated descriptions can be found in Table E.

[0176] In certain aspects, the description provides a microorganism that contains a sequence with a non-natural compound that is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78% , 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95 %, 96%, 97%, 98%, 99% or 100% identical to the sequence with SEQ ID NO: 372-405. These sequences and their associated descriptions can be found in Table E.

[0177] In certain aspects, the description provides a microorganism that contains an amino acid sequence that is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79 %, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identical to SEQ ID NO: 77, 78, 81, 82, or 83. These sequences and their associated descriptions can be found in Tables F and H.

Genetic variation - Detection methods: primers, probes and assays

[0178] In the present description, primers, probes and assays are indicated that are suitable for the detection of microorganisms investigated in this document. In certain aspects, the disclosure provides methods for detecting parental wild-type strains. In other aspects, the disclosure provides methods for detecting non-intergeneric genetically engineered microorganisms derived from wild-type strains. In certain aspects, the present invention relates to methods for identifying non-intergeneric genetic alterations in a microorganism.

[0179] In certain aspects, the genetic engineering methods of the present invention result in sequences with non-natural "connection" of nucleotides in non-intergeneric microorganisms. These non-natural nucleotide compounds can be used as a form of diagnosis that is indicative of the presence of a specific genetic alteration in the microorganism investigated herein.

[0180] The methods of the present invention can detect these non-natural nucleotide compounds using specialized quantitative PCR methods, including uniquely designed primers and probes. In certain aspects, the probes of the invention bind to non-naturally coupled sequences. In certain aspects, conventional PCR is used. In other aspects, real-time detection PCR is used. In certain aspects, quantitative PCR (qPCR) is used.

[0181] Thus, the description may include the use of two general methods for detecting real-time PCR products: (1) non-specific fluorescent dyes that intercalate any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides labeled a fluorescent reporter that provides detection only after hybridization of the probe with its complementary sequence. In certain aspects, only a non-natural nucleotide compound can be amplified by said primers, and thus can be detected by a non-specific dye or by using a specific hybridization probe. In other aspects, the primers of the invention are chosen such that the primers flank either side of the sequence with the compound so that if an amplification reaction occurs, then the specified sequence is present with the compound.

[0182] Aspects of the invention include non-natural nucleotide pairing sequence molecules themselves, together with other nucleotide molecules that are capable of binding to said non-natural nucleotide pairing sequences under moderate to stringent hybridization conditions. In certain aspects, nucleotide molecules that are capable of binding to said sequences with unnatural nucleotide bonding under moderate to stringent conditions are referred to as "nucleotide probes".

[0183] In certain aspects, genomic DNA can be isolated from samples and used to quantify the presence of microorganisms of the invention using qPCR. The primers used in the qPCR reaction can be primers designed with Primer Blast (https://www.ncbi.nlm.nih.gov/tools/primer-blast/) to amplify unique regions of the wild-type genome or unique regions that have been genetically modified. engineering of non-intergeneric mutant strains. The qPCR reaction can be performed using the SYBR GreenER qPCR SuperMix Universal kit (Thermo Fisher P/N 11762100) using only forward and reverse amplification primers; alternatively, the Kapa Probe Force kit (Kapa Biosystems P/N KK4301) can be used with amplification primers and a TaqMan probe containing a FAM dye tag at the 5' end, an internal ZEN quencher, and a minor groove binder and a fluorescent quencher at the 3' end. end (Integrated DNA Technologies).

[0184] Certain sequences of primers, probes, and sequences with non-natural compounds are shown in Table E. The efficiency of the qPCR reaction can be determined using a standard curve derived from a known amount of gDNA from the target genome. The data can be normalized to the number of copies of the genome per g wet weight using tissue weight and isolation volume.

[0185] Quantitative polymerase chain reaction (qPCR) is a method for quantitative, with real-time detection, amplification of one or more nucleic acid sequences. Real-time quantification in the PCR assay provides quantification of nucleic acids produced by the PCR amplification steps by comparing the amplification of the nucleic acids of interest and a corresponding control nucleic acid sequence that can act as a calibration standard.

[0186] TaqMan probes are often used in qPCR assays, which require increased specificity to quantify target nucleic acid sequences. TaqMan probes contain an oligonucleotide probe with a fluorophore attached to the 5' end and a quencher attached to the 3' end of the probe. When the TaqMan probes are left as is, with the 5' and 3' ends of the probe in close contact with each other, the quencher prevents transmission of the fluorescent signal from the fluorophore. TaqMan probes are designed to anneal to the region of the nucleic acid amplified with a specific set of primers. Since Taq polymerase extends the primer and synthesizes the nascent chain, the 5'-3' exonuclease activity of Taq polymerase destroys the probe that has annealed to the template. This destruction of the probe releases the fluorophore, thus breaking close contact with the quencher and allowing the fluorophore to fluoresce. The fluorescence detected in the qPCR assay is directly proportional to the fluorophore released and the amount of DNA template present in the reaction.

[0187] The properties of qPCR allow the practitioner to eliminate the laborious step of post-amplification of the gel electrophoresis preparation, which is usually necessary to monitor the amplified products of traditional PCR assays. The advantages of qPCR over conventional PCR are significant and include increased speed, ease of use, reproducibility, and quantitation.

Property Improvement

[0188] The methods of the present invention can be used to impart or improve one or more of a number of desired features. Examples of traits that can be given or improved include: root biomass, root length, growth, shoot height, number of leaves, water use efficiency, total biomass, yield, fruit size, seed size, photosynthesis rate, drought tolerance, heat tolerance, salt tolerance, tolerance nematode stress, fungal pathogen resistance, bacterial pathogen resistance, viral pathogen resistance, metabolite level and proteome expression. Required traits including growth, total biomass, root and/or shoot biomass, seed germination, seedling viability, photosynthetic efficiency, transcription rate, seed/fruit number or weight, plant seed or fruit yield, leaf chlorophyll content, photosynthesis rate, length roots, or any combination thereof, can be used to determine growth and compare with the growth rate of reference crop plants (eg, plants without improved traits) grown under identical conditions.

[0189] The preferred attribute to be imparted or improved, as described herein, is nitrogen fixation. In some instances, plants produced by the methods described herein exhibit trait differences that are at least about 5%, such as at least about 5%, at least about 8%, over at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 75%, at least about 80%, at least about 80%, at least about 90%, or at least 100%, at least about 200%, at least about 300%, at least about 400% or more compared to a reference crop grown in soil under the same conditions. In additional examples, a plant produced by the methods described herein exhibits trait differences that are at least about 5% greater, such as at least about 5%, at least about 8%, at least about 10 %, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 40%, at least about 50%, at least about 60% , at least about 75%, at least about 80%, at least about 80%, at least about 90%, or at least 100%, at least about 200%, at least about 300%, at least about 400% or more than a reference crop grown under similar soil conditions.

[0190] An improved trait can be evaluated under conditions involving exposure to one or more biotic or abiotic stressors. Examples of stressors include abiotic stressors (such as heat stress, salt stress, drought stress, cold stress, and nutrient deficiency stress) and biotic stressors (such as nematode stress, herbivorous insect stress, stress, stress caused by fungal pathogens, stress caused by bacterial pathogens and stress caused by viral pathogens).

[0191] The trait improved by the methods and compositions of the present invention may be nitrogen fixation, including in a plant previously incapable of nitrogen fixation. In some cases, bacteria isolated by the method described herein produce 1% or more (for example, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15% , 20% or more) of plant nitrogen, which may represent an increase in nitrogen fixation capacity of at least 2-fold (e.g., 3-fold, 4-fold, 5-fold, 6-fold, 7-fold, 8-fold , 9-fold, 10-fold, 20-fold, 50-fold, 100-fold, 1000-fold or more) compared to bacteria isolated from the first plant before any genetic variation was introduced. In some cases, the bacteria produce 5% or more of the plant's nitrogen. The desired level of nitrogen fixation can be achieved by repeating the steps of introducing genetic variation, exposing multiple plants, and isolating bacteria from trait-improved plants (e.g., 1, 2, 3, 4, 5, 10, 15, 25 or more times) once or multiple times. . In some cases, increased levels of nitrogen fixation are achieved in the presence of a fertilizer supplemented with glutamine, ammonia, or other chemical nitrogen source. Known methods for assessing the degree of nitrogen fixation, examples of which are described in this document.

[0192] Breeding microorganisms is a method for systematically identifying and improving the role of species in the crop microbiome. The method includes three steps: 1) selection of candidate species by mapping plant-microorganism interactions and predicting a regulatory network linked to a particular phenotype, 2) practical and predictable improvement of microorganism phenotypes by intraspecific crossing of regulatory networks and gene clusters, and 3) screening and selection of new genotypes of microorganisms that provide the required phenotypes of crops. For a systematic analysis of the improvement of strains, a model was obtained in which the dynamics of colonization of a community of microorganisms is linked to the genetic activity of key species. The model is used to predict the breeding of genetic targets and to increase the frequency of selection for improvements in microbiome-encoded properties of agricultural value.

Determination of nitrogen delivered in agriculturally significant field conditions

[0193] In the field, the amount of nitrogen delivered can be determined as a function of colonization times activity.

[0194] The above equation requires (1) average colonization per unit of plant tissue and (2) activity in terms of the amount of fixed nitrogen or the amount of ammonia excreted by each cell of the microorganism. To convert to pounds of nitrogen per acre, the physiology of corn growth over time is tracked, such as plant size and associated root system, at all stages of maturity.

[0195] The pounds of nitrogen delivered to the crop per acre/season can be calculated using the following equation:

[0196] Plant tissue (t) is the wet mass of corn plant tissue during growth time (t). Values for making a reasonable calculation are detailed in Roots, Growth and Nutrient Uptake (Mengel. Dept. of Agronomy Pub. # AGRY-95-08 (Rev. May-95. p. 1-8).

[0197] Colonization (t) is the number of microorganisms of interest found in plant tissue per gram wet weight of plant tissue, at any particular time, t, during a growth season. In the case where only one time point is available, that one time point is normalized as the peak colonization rate per season, and the colonization rate at the remaining time points is thus adjusted.

[0198] Activity(t) is the rate at which microorganisms of interest fix N per unit time, at any particular time, t, during a growth season. In the embodiments described herein, this level of activity is approximated by an in vitro acetylene reduction (ARA) assay in ARA media in the presence of 5 mM glutamine or an ammonium excretion assay in ARA media in the presence of 5 mM ammonium ions.

[0199] The delivered amount of nitrogen is then calculated by numerically integrating the above function. In cases where the values of the variables described above are discretely determined at specified time points, the values between these time points are approximated by linear interpolation.

Nitrogen fixation

[0200] Described herein are methods of increasing nitrogen fixation in a plant, comprising exposing the plant to bacteria carrying one or more genetic variations introduced into one or more genes regulating nitrogen fixation, wherein the bacteria produce 1% or more nitrogen in the plant (e.g. , 2%, 5%, 10% or more), which may represent at least 2 times the nitrogen fixation capacity compared to the plant in the absence of bacteria. Bacteria can produce nitrogen in the presence of a fertilizer supplemented with glutamine, urea, nitrates, or ammonia. Genetic variations can be any of the genetic variations described herein, including the examples above, in any amount and in any combination. Genetic variation can be introduced into a gene selected from the group consisting of nifA, nifL, ntrB, ntrC, glutamine synthetase, glnA, glnB, glnK, draT, amtB, glutaminase, glnD, glnE, nifJ, nifH, nifD, nifK, nifY, nifE , nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, and nifQ. The genetic variation may be a mutation that results in one or more of: increased expression or activity of NifA or glutaminase; reduced expression or activity of NifL, NtrB, glutamine synthetase, GlnB, GlnK, DraT, AmtB; reduced GlnE adenylate scavenging activity; or reduced GlnD uridylate scavenging activity. A genetic variation introduced into one or more bacteria by the methods described herein may be a knockout mutation, or it may remove the regulatory sequence of the target gene, or it may include the insertion of a heterologous regulatory sequence, for example, the insertion of a regulatory sequence identified in bacterial genome of the same species or genus. The regulatory sequence can be selected based on the level of gene expression in the bacterial culture or plant tissue. Genetic variation can be obtained by chemical mutagenesis. Plants grown in step (c) may be subjected to biotic or abiotic stress factors.

[0201] Nitrogen fixation occurring in the plants described herein can be quantified in various ways, for example, by analyzing acetylene reduction (AR). The acetylene reduction assay can be performed in vitro or in vivo . Evidence that a particular bacterium provides fixed nitrogen to the plant may include: 1) the total N of the plant significantly increases after inoculation, preferably with a concomitant increase in N concentration in the plant; 2) symptoms of nitrogen deficiency under N-limiting conditions improve after inoculation (which should include an increase in dry weight); 3) documentation of N ₂ fixation using the ¹⁵ N approach (which may be isotope dilution experiments, ¹⁵ N ₂ recovery assays, or natural ¹⁵ N abundance assays); 4) the incorporation of fixed N into the plant protein or metabolite; and 5) the absence of all these effects in plants without inoculation or in plants inoculated with a mutant of the inoculated strain.

[0202] The wild-type nitrogen fixation regulatory cascade can be thought of as a digital logic circuit where incoming O ₂ and NH ₄ ⁺ are passed through a NOR logic gate whose output, in addition to ATP, goes to an AND logic gate. In certain embodiments, the methods, described here disrupt the action of NH ₄ ⁺ on this circuit at several points in the regulatory cascade so that microorganisms can produce nitrogen even in fertilized fields. However, the methods described herein also involve altering the effect of ATP or O ₂ on the schema, or altering the schema using other regulatory cascades in the cell, or altering genetic circuits other than nitrogen fixation. It is possible to rearrange gene clusters to obtain functional products under the control of a heterologous regulatory system. By removing natural regulatory elements outside and in the coding sequences of gene clusters and replacing them with alternative regulatory systems, the functional products of complex genetic operons and other gene clusters can be controlled and/or placed in heterologous cells, including cells of other species than those from which the natural genes. Once rearranged, the synthetic gene clusters can be controlled by genetic circuits or other inducible regulatory systems, thus controlling the expression of the products as desired. Expression cassettes can be designed to act as logic gates, pulse generators, oscillators, switches, or memory devices. The expression control cassette can be ligated to the promoter such that the expression cassette functions as an environmental sensor such as oxygen, temperature, touch, osmotic stress, surface tension, or redox.

[0203] As an example, the nifL, nifA, nifT, and nifX genes can be deleted from the nif gene cluster. Synthetic genes can be constructed by randomizing the codons of the DNA encoding each amino acid sequence. The selection of codons is carried out by establishing that the frequency of use of codons is as different as possible from the frequency of use of codons in the natural gene. The proposed sequences are scanned for any undesirable features such as restriction enzyme recognition sites, transposon recognition sites, repeat sequences, sigma 54 and sigma 70 promoters, cryptic ribosome binding sites, and rho-independent terminators. Synthetic ribosome binding sites are chosen to match the strength of each corresponding natural ribosome binding site, for example by constructing a fluorescent reporter plasmid in which the 150 bp surrounding the gene's start codon (-60 to +90) is fused to the gene fluorescent marker. This chimeric gene can be expressed under the control of the Ptac promoter and fluorescence measured by flow cytometry. To obtain synthetic ribosome binding sites, a reporter plasmid library is prepared using 150 bp. (-60 to +90) synthetic expression cassette. Briefly, a synthetic expression cassette may consist of a random DNA spacer, a degenerate sequence encoding an RBS library, and the coding sequence for each synthetic gene. Multiple clones are screened to identify a synthetic ribosome binding site that best matches the natural ribosome binding site. Thus, synthetic operons are constructed that consist of the same genes as natural operons and tested for functional complementation. An additional illustrative description of synthetic operons is given in US20140329326.

Types of bacteria

[0204] Microorganisms useful in the methods and compositions described herein can be obtained from any source. In certain instances, the microorganisms may be bacteria, archaea, protozoa, or fungi. The microorganisms of the present disclosure may be nitrogen-fixing microorganisms, for example, nitrogen-fixing bacteria, nitrogen-fixing archaea, nitrogen-fixing fungi, nitrogen-fixing yeasts, or nitrogen-fixing protozoa. Microorganisms useful in the methods and compositions described herein may be spore-forming microorganisms, eg spore-forming bacteria. In certain instances, bacteria useful in the methods and compositions described herein may be gram positive bacteria or gram negative bacteria. In certain instances, the bacteria may be the endospore-forming bacteria Firmicute phylum . In certain cases, the bacteria may be a diazatroph. In certain cases, bacteria may not be diazotrophic.

[0205] The methods and compositions of the present disclosure can be used using archaea such as, for example, Methanothermobacter thermoautotrophicus .

[0206] In certain instances, bacteria that may be useful include, but are not limited to , Agrobacterium radiobacter , Bacillus acidocaldarius , Bacillus acidoterrestris , Bacillus agri , Bacillus aizawai , Bacillus albolactis , Bacillus alcalophilus , Bacillus alvei , Bacillus aminoglucosidicus , Bacillus a minovorans , Bacillus amylolyticus (also known as Paenibacillus amylolyticus ) Bacillus amyloliquefaciens , Bacillus aneurinolyticus , Bacillus atrophaeus , Bacillus azotoformans , Bacillus badius , Bacillus cereus (synonyms: Bacillus endorhythmos , Bacillus medusa ), Bacillus chitinosporus , Bacillus circulans , Bac illus coagulans , Bacillus endoparasiticus Bacillus fastidiosus , Bacillus firmus Bacillus kurstaki Bacillus lacticola Bacillus lactimorbus acillus mycoides , Bacillus natto , _ _ _ Bacillus nematocida Bacillus nigrificans Bacillus nigrum Bacillus pantothenticus Bacillus popillae Bacillus psychrosaccharolyticus Bacillus pumilus Bacillus siamensis Bacillus smithii Bacillus sphaericus Bacillus subtilis Bacillus thuringiensis Bacillus uniflag ellatus , Bradyrhizobium japonicum , Brevibacillus brevis Brevibacillus laterosporus (formerly Bacillus laterosporus ), Chromobacterium subtsugae , Delftia acidovorans , Lactobacillus acidophilus , Lysobacter antibioticus , Lysobacter enzymogenes , Paenibacillus alvei , Paenibacillus polymyxa , Paenibacillus popilliae (formerly Bacillus popilliae ), Pantoea agglomerans , Pasteuria penetrans (formerly Bacillus penetrans ), Pasteuria usgae , Pectobacterium carotovorum (formerly Erwinia carotovora ) Pseudomonas aeruginosa Pseudomonas aureofaciens Pseudomonas cepacia a , Serratia marcescens , Streptomyces colombiensis , Streptomyces galbus , Streptomyces goshikiensis , Streptomyces griseoviridis , Streptomyces lavendulae , Streptomyces prasinus , Streptomyces saraceticus , Streptomyces venezuelae , Xanthomonas campestris , Xenorhabdus luminescens , Xenorhabdus nematophila , Rhodococcus globerulus AQ719 (NRRL accession no. B-21663), species B acillus AQ175 (ATCC Accession No. 55608), species Bacillus AQ 177 ( Accession No. ATCC 55609), Bacillus species (Accession No. ATCC 53522) and Streptomyces species strain Accession No. NRRL B-30145. In certain instances, the bacterium may also be Azotobacter chroococcum , Methanosarcina barkeri , Klesiella pneumoniae , Azotobacter vinelandii , Rhodobacter spharoides , Rhodobacter capsulatus , Rhodobcter palustris , Rhodosporillum rubrum , Rhizobium leguminosarum , or Rhizobium etli .

[0207] In certain instances, the bacterium may be a Clostridium spp., for example, Clostridium pasteurianum , Clostridium beijerinckii , Clostridium perfringens , Clostridium tetani , Clostridium acetobutylicum .

[0208] In certain cases, the bacteria used in the methods and compositions of the present invention may be cyanobacteria. Examples of cyanobacteria genera include Anabaena (eg Anagaena species PCC7120), Nostoc (eg Nostoc punctiforme ) or Synechocystis (eg Synechocystis species PCC6803).

[0209] In certain cases, the bacteria used in the methods and compositions of the present invention may belong to the Chlorobi taxonomic group, for example, Chlorobium tepidum .

[0210] In certain cases, the microorganisms used in the methods and compositions of the present invention may contain a gene homologous to a known NifH gene. Sequences of known NifH genes can be found, for example, in the database of J. Zehr's laboratory NifH, (https://wwwzehr.pmc.ucsc.edu/nifH_Database_Public/, April 4, 2014), or the database of D. Buckley's laboratory NifH (http ://www.css.cornell.edu/faculty/buckley/nifh.htm, and Gaby, John Christian, and Daniel H. Buckley "A comprehensive aligned nifH gene database: a multipurpose tool for studies of nitrogen-fixing bacteria" .Database 2014 (2014): bau001.). In certain cases, the microorganisms used in the methods and compositions of the present invention may contain a sequence that encodes a polypeptide for at least 60%, 70%, 80%, 85%, 90%, 95%, 96%, 96%, 98 %, 99% or more than 99% sequence identical with the sequence from the NifH database of the laboratory of J. Zehr, (https://wwwzehr.pmc.ucsc.edu/nifH_Database_Public/, April 4, 2014). In certain cases, the microorganisms used in the methods and compositions of the present invention may contain a sequence that encodes a polypeptide for at least 60%, 70%, 80%, 85%, 90%, 95%, 96%, 96%, 98 %, 99% or more 99% sequence identical with the sequence from the NifH database of the laboratory of D. Buckley, (Gaby, John Christian, and Daniel H. Buckley. "A comprehensive aligned nifH gene database: a multipurpose tool for studies of nitrogen- fixing bacteria". Database 2014 (2014): bau001.).

[0211] Microorganisms useful in the methods and compositions described herein can be obtained by isolating microorganisms from the surfaces or tissues of natural plants; crushing seeds with the release of microorganisms; planting seeds in various soil samples and isolating microorganisms from tissues; or inoculating plants with exogenous microorganisms and determining which microorganisms are found in plant tissues. Non-limiting examples of plant tissues include seeds, shoots, leaves, sections, plants, bulbs, or tubers. In some cases, bacteria are isolated from seeds. Sample processing parameters can be varied to isolate different types of associated microorganisms such as rhizospheric microorganisms, epiphytes or endophytes. Instead of initial isolation from the first plant, the bacteria can also be obtained from repositories such as collections of natural strains. Microorganisms can be genotyped and phenotyped by sequencing the genomes of isolated microorganisms; profiling the composition of communities in planta ; characteristics of the functionality of transcriptomes of communities or isolated microorganisms; or screening for microbial properties using selective or phenotypic media (eg, nitrogen-fixing or phosphate-dissolving phenotypes). Selected strains or candidate populations can be derived from sequencing data; phenotyping data; plant data (eg genome, phenotype and/or yield data); soil data (eg, pH, N/P/K content, and/or biotic community of the base soil), or any combination of these.

[0212] The bacteria and methods for producing bacteria described herein can be used for bacteria that are capable of efficient self-propagation on leaf surfaces, root surfaces, or within plant tissues without inducing a destructive plant defense response, or for bacteria that are resistant to defense plant reactions. The bacteria described herein can be isolated by culturing a plant tissue extract or by washing the leaf surface in a medium without the addition of nitrogen. However, the bacteria may be non-culturable, ie. their culturability may not be known, or they may be difficult to cultivate by standard methods known in the art. The bacteria described herein may be endophytic or epiphytic, or bacteria living in the rhizosphere of a plant (rhizosphere bacteria). Bacteria obtained after repeating the steps of introducing genetic variation, exposing multiple plants, and isolating bacteria from plants with an improved trait (e.g., 1, 2, 3, 4, 5, 10, 15, 25, or more times) may be endophytic , epiphytic or rhizospheric. Endophytes are organisms that enter the interior of plants without causing disease symptoms or causing the formation of symbiotic structures, and are of agronomic interest because they can enhance plant growth and improve plant nutrition (eg, through nitrogen fixation). The bacteria may be seed-borne endophytes. Seed-borne endophytes include bacteria associated with or derived from seeds of grasses or other plants, such as seed-borne bacterial endophytes found in mature, dry, undamaged (e.g., no cracks, no visible fungal infection, or no premature germination) seeds . Seed-borne bacterial endophytes may be associated or obtained from the surface of seeds; alternatively, or in addition, they may be associated or may be obtained from the internal compartment of the seed (eg surface-sterilized seeds). In certain cases, seed-borne bacterial endophytes are able to replicate in plant tissues, for example, within seeds. In certain cases, seed-borne bacterial endophytes are also able to survive dehydration.

[0213] Bacteria isolated by the methods of the invention or used in the methods or compositions of the invention may include a variety of bacteria of different taxa in combination. By way of example, bacteria may include Proteobacteria (such as Pseudomonas, Enterobacter, Stenotrophomonas, Burkholderia, Rhizobium, Herbaspirillum, Pantoea, Serratia, Rahnella, Azospirillum, Azorhizobium, Azotobacter, Duganella, Delftia, Bradyrhizobiun, Sinorhizobium and Halomonas), Firmicutes ( such as bacillus , Paenibacillus, Lactobacillus, Mycoplasma and Acetabacterium) and Actinobacteria (such as Streptomyces, Rhodacoccus, Microbacterium and Curtobacterium) . The bacteria used in the methods and compositions of the present disclosure may include nitrogen-fixing bacterial consortia of two or more species. In certain cases, one or more species of bacteria of bacterial consortia may be capable of nitrogen fixation. In certain cases, one or more types of bacterial consortia can facilitate or increase the ability of other bacteria to fix nitrogen. Bacteria that fix nitrogen and bacteria that increase the ability of other bacteria to fix nitrogen may be the same or different. In certain instances, a bacterial strain may be capable of nitrogen fixation when in combination with another bacterial strain or in certain bacterial consortia, but may be incapable of nitrogen fixation in a monoculture. Examples of bacterial genera that can be found in nitrogen-fixing bacterial consortia include, but are not limited to, Herbaspirillum , Azospirillum , Enterobacter , and Bacillus .

[0214] Bacteria that can be obtained by the methods described herein include Azotobacter spp ., Bradyrhizobium spp. , Klebsiella spp., and Sinorhizobium spp . In certain cases, the bacteria can be selected from the group consisting of: Azotobacter vinelandii, Bradyrhizobium japonicum, Klebsiella pneumoniae and Sinorhizobium meliloti . In certain instances, the bacteria may be of the genus Enterobacter or Rahnella . In certain instances, the bacteria may be of the genus Frankia or Clostridium . Examples of bacteria of the genus Clostridium include, but are not limited to, Clostridium acetobutilicum , Clostridium pasteurianum , Clostridium beijerinckii , Clostridium perfringens , and Clostridium tetani . In certain cases, the bacteria may be of the genus Paenibacillus , e.g., Paenibacillus azotofixans , Paenibacillus borealis , Paenibacillus durus , Paenibacillus macerans , Paenibacillus polymyxa , Paenibacillus alvei , Paenibacillus amylolyticus , Paenibacillus campinasensis , Paenibacillus chibensis , Pa enibacillus glucanolyticus , Paenibacillus illinoisensis , Paenibacillus larvae subsp. Larvae , Paenibacillus larvae subsp. Pulvifaciens , Paenibacillus lautus , Paenibacillus macerans , Paenibacillus macquariensis , Paenibacillus macquariensis , Paenibacillus pabuli , Paenibacillus peoriae , or Paenibacillus polymyxa .

[0215] In certain examples, bacteria isolated by the methods of the invention may be members of one or more of the following taxa: Achromobacter , Acidithiobacillus , Acidovorax , Acidovoraz , Acinetobacter , Actinoplanes , Adlercreutzia , Aerococcus , Aeromonas , Afipia , Agromyces , Ancylobacter , Arthrobacter , Atopost ipes , Azospirillum , Bacillus , Bdellovibrio , Beijerinckia , Bosea , Bradyrhizobium , Brevibacillus , Brevundimonas , Burkholderia , Candidatus Haloredivivus , Caulobacter , Cellulomonas , Cellvibrio , Chryseobacterium , Citrobacter , Clostridium , Coraliomargarita , Corynebacterium , Cupriavidus , Curtobacterium , Curvibacter , Deinococcus , Delftia , Desemzia , Devosia , Dokdonella , Dyella , Enhydrobacter , Enterobacter , Enterococcus , Erwinia , Escherichia , Escherichia/Shigella , Exiguobacterium , Ferroglobus , Filimonas , Finegoldia , Flavisolibacter , Flavobacterium , Frigoribacterium , Gluconacetobacter , Hafnia , Halobaculum , Halomonas , Halosimplex , Herbaspirillum , Hymenobacter , Klebsiella , Kocuria , Kosakonia , Lactobacillus , Leclercia , Lentzea , Luteibacter , Luteimonas , Massilia , Mesorhizobium , Methylobacterium , Microbacterium , Micrococcus , Microvirga , Mycobacterium , Neisseria , Nocardia , Oceanibaculum , Ochrobactrum , Okibacterium , Oligotropha , Oryzihumus , Oxalophagus , Paenibacillus , Panteoa , Pantoea , Pelomonas , Perlucidibaca , Plantibacter , Polynucleobacter , Propionibacterium , Propioniciclava , Pseudoclavibacter , Pseudomonas , Pseudonocardia , Pseudoxanthomonas , Psychrobacter , Rahnella , Ralstonia , Rheinheimera , Rhizobium , Rhodococcus , Rhodops eudomonas , Roseateles , Ruminococcus , Sebaldella , Sediminibacillus , Sediminibacterium , Serratia , Shigella , Shinella , Sinorhizobium , Sinosporangium , Sphingobacterium , Sphingomonas , Sphingopyxis , Sphingosinicella , Staphylococcus , 25 Stenotrophomonas , Strenotrophomonas , Streptococcus , Streptomyces , Stygiolobus , Sulfurisphaera , Tatumella , Tepidimonas , Thermomonas , T hiobacillus , Variovorax , genera WPS-2 indeterminate, Xanthomonas and Zimmermannella .

[0216] In certain instances, bacterial species selected from at least one of the following genera are used: Enterobacter , Klebsiella , Kosakonia , and Rahnella . In certain cases, a combination of bacterial species of the following genera is used: Enterobacter, Klebsiella, Kosakonia and Rahnella . In certain cases, the species used may be one or more of: Enterobacter sacchari , Klebsiella variicola , Kosakonia sacchari and Rahnella aquatilis .

[0217] In certain cases, a gram-positive microorganism may contain a molybdenum-iron nitrogenase system containing: nifH , nifD , nifK , nifB , nifE , nifN , nifX , hesA , nifV , nifW , nifU , nifS , nifI1 and nifI ₂ . In certain cases, a gram-positive microorganism may contain a vanadium nitrogenase system containing: vnfDG , vnfK , vnfE , vnfN , vupC , vupB , vupA , vnfV , vnfR1 , vnfH , vnfR2 , vnfA (transcriptional regulator). In certain cases, a gram-positive microorganism may have an iron-only nitrogenase system comprising: anfK , anfG , anfD , anfH , anfA (transcriptional regulator). In certain cases, a gram-positive microorganism may contain a nitrogenase system containing glnB and glnK (proteins that transmit a signal from nitrogen). Specific examples of enzymes involved in nitrogen metabolism in Gram-positive microorganisms include glnA (glutamine synthetase), gdh (glutamate dehydrogenase), bdh (3-hydroxybutyrate dehydrogenase), glutaminase, gltAB/gltB/gltS (glutamate synthase), asnA/asnB (aspartate ammine ligase/asparagine synthetase) and ansA /ansZ (asparaginase). Specific examples of proteins involved in nitrogen transport in Gram-positive microorganisms include amtB (ammonium transporter), glnK (ammonium transport regulator), glnPHQ/glnQHMP (ATP-dependent glutamine/glutamate transporters), glnT/alsT/yrbD/yflA (glutamine-like proton transporters) and gltP/gltT/yhcl/nqt (glutamine-like proton co-transporters).

[0218] Examples of Gram-positive microorganisms that may be of particular interest include Paenibacillus polymixa , Paenibacillus riograndensis , Paenibacillus spp., Frankia spp., Heliobacterium spp ., Heliobacterium chlorum , Heliobacillus spp., Heliophilum spp ., Heliorestis spp ., Clostridium acetobutylicum , Clostridium spp ., Mycobact erium flaum , Mycobacterium spp ., Arthrobacter spp ., Agromyces spp. , Corynebacterium autitrophicum , Corynebacterium spp. , Micromonspora spp. , Propionibacteria spp ., Streptomyces spp . and Microbacterium spp .

[0219] Certain examples of genetic alterations that can be carried out in Gram-positive microorganisms include: glnR deletion to deregulate BNF in the presence of environmental nitrogen, insertion of different promoters just upstream of the nif cassette to deregulate GlnR in response to environmental nitrogen, mutation glnA with a decrease in the level of ammonium assimilation along the GS-GOGAT pathway, deletion of amtB with a decrease in ammonium consumption from the environment, mutation of glnA so that it is constitutively in a state of feedback inhibition (FBI-GS), with a decrease in ammonium assimilation along the GS- GOGAT.

[0220] In certain cases, glnR is the main regulator of N metabolism and fixation in Paenibacillus species. In certain cases, the genome of Paenibacillus species may not contain a gene for the production of glnR. In certain cases, the genome of the Paenibacillus species may not contain a gene for the production of glnE or glnD. In certain cases, the genome of Paenibacillus species may contain a gene for the production of glnB or glnK . For example, the species Paenibacillus WLY78 does not contain the gene for glnB or its homolog found in the archaebacterium Methanococcus maripaludis , nifI1 and nifI ₂ . In certain cases, the genomes of Paenibacillus species may vary. For example, Paenibacillus polymixa E681 lacks glnK and gdh , and has several nitrogen compound transporters, but apparently only amtB is under the control of GlnR. In another example, the species Paenibacillus JDR2 contains glnK , gdh and most of the other central nitrogen metabolism genes, contains many smaller nitrogen compound transporters, but does not contain glnPHQ under the control of GlnR. Paenibacillus riograndensis SBR5 contains the glnRA standard operon, the fdx gene, the nif primary operon, the nif secondary operon, and the anf operon (coding for iron-only nitrogenase). Upstream of each of these operons, putative glnR/tnrA regions have been identified. GlnR can regulate all of the above operons except for the anf operon. GlnR can bind to each of these regulatory sequences as a dimer.

[0221] N-fixing strains of Paenibacillus can fall into two subgroups: subgroup I, which contains only a minimal cluster of nif genes, and subgroup II, which contains a minimal cluster and an uncharacterized gene between nifX and hesA , and duplication of certain nif genes often occurs in other clusters. such as nifH , nifHDK , nifBEN, or clusters encoding vanadium ( vnf ) or iron-only nitrogenase nitrogenase genes).

[0222] In certain cases, the genome of Paenibacillus species may not contain a gene for the production of glnB or glnK . In certain cases, the genome of Paenibacillus species may contain a minimal nif cluster with 9 genes transcribed from the sigma-70 promoter. In certain cases, a cluster of nif Paenibacillus can negatively regulate nitrogen or oxygen. In certain cases, the genome of Paenibacillus species may not contain the gene for the production of sigma-54 . For example, the species Paenibacillus WLY78 does not contain the sigma-54 gene. In certain cases, the nif cluster may regulate glnR and/or TnrA. In certain cases, the activity of the nif cluster can be determined by changing the activity of glnR and/or TnrA.

[0223] In Bacilli, glutamine synthetase (GS) is feedback inhibited by high concentrations of intracellular glutamine, causing a shift in confirmation (referred to as FBI-GS). The Nif clusters contain prominent binding sites for the GlnR and TnrA regulators in various Bacilli species. GlnR binds and represses gene expression in the presence of excess intracellular glutamine and AMP. The role of GlnR may be to prevent the influx and intracellular production of glutamine and ammonium under conditions of high nitrogen availability. TnrA can bind and/or activate (or repress) gene expression in the presence of limiting intracellular glutamine and/or in the presence of FBI-GS. In certain cases, the activity of the nif Bacilli cluster can be changed by changing the activity of GlnR.

[0224] Feedback-inhibited glutamine synthetase (FBI-GS) can bind GlnR and stabilize GlnR binding to recognized sequences. Several bacterial species contain a GlnR/TnrA binding site upstream of the nif cluster. Altering FBI-GS and GlnR binding can alter the activity of the nif pathway.

Sources of microorganisms

[0225] Bacteria (or any microorganism as described) can be obtained from any common terrestrial environment, including its soils, plants, fungi, animals (including invertebrates) and other biota, including sediment, water, and biota of lakes and rivers; from the marine environment, its biota and sediments (eg sea water, sea silt, marine plants, marine invertebrates (eg sponges), marine vertebrates (eg fish)); terrestrial and marine geosphere (loose surface sediments and stones, for example, crushed subsoil stones, sand and clays); the cryosphere and its melt waters; atmosphere (for example, filtered suspended dust, cloud and rain drops); urban, industrial, and other man-made environments (eg, accumulated organic and mineral material on concrete, curbs, roof surfaces, and road surfaces).

[0226] The plants from which the bacteria (or any microorganism as described) are derived can be a plant with one or more of the desired traits, such as plants that naturally grow in a particular environment or under certain conditions of interest. As an example, a particular plant may naturally grow in sandy soil or sand with a high mineral content, or at extreme temperatures, or with little water, or it may be resistant to certain pests or diseases present in the environment, and it may be desirable growing a commercial crop under such conditions, in particular, for example, if they are the only conditions available in a particular geographical location. As a further example, the bacteria can be isolated from a commercial crop grown in such media, or more specifically, from a particular crop in which, among crops grown in any particular environment, the trait of interest is best represented: e.g., fastest growing plants among crops grown in soils with limited salt levels, or the least damaged plants among crops that have been severely damaged by insects or epidemic disease, or plants with the required amounts of certain metabolites and other compounds, including fiber content, oil content, etc. , or plants that exhibit the required colors, tastes or smells. Bacteria can be isolated from the plant of interest or any material found in the environment of interest, including fungi and other animal and plant biota, soil, water, sediments, and other environmental components, as previously discussed.

[0227] Bacteria (or any microorganism as described) can be isolated from plant tissue. This isolation can be carried out from any suitable tissue of the plant, including, for example, the root, stem and leaves, and reproductive tissues of the plant. By way of example, conventional plant isolation methods typically involve sterile cutting of test plant material (e.g., root or stem sections, leaves), sterilizing the surface with a suitable solution (e.g., 2% sodium hypochlorite), and then placing the plant material on nutrient medium for the growth of microorganisms. Alternatively, the surface-sterilized plant material can be ground into a sterile liquid (typically water) and a liquid suspension containing small portions of the ground plant material applied to the surface of a suitable solid agarose medium or media, which may or may not be selective (e.g., contain only phytic acid). acid as a source of phosphorus). This approach is particularly useful for bacteria that form isolated colonies and that can be individually transferred to individual culture media plates and further purified to a single species by well-known methods. Alternatively, samples of the roots or leaves of the plant may not be surface-sterilized but only gently washed, thus including surface-dwelling epiphytic microorganisms in the isolation process, or epiphytic microorganisms may be isolated separately by applying and removing parts of the roots, stems or leaves of plants on the surface. agarose medium and then isolating individual colonies as described above. This approach is particularly suitable, for example, for bacteria. Alternatively, the roots can be treated without washing off the small amounts of soil adhering to the roots, thus including the microorganisms that colonize the plant's rhizosphere. In other cases, the soil adhering to the roots can be taken, diluted and applied to agar with suitable selective and non-selective media to obtain individual colonies of rhizospheric bacteria.

BUDAPEST TREATY ON THE INTERNATIONAL RECOGNITION OF THE DEPOSIT OF MICROORGANISMS FOR THE PURPOSE OF PATENT PROCEDURE

[0228] Deposits of microorganisms of the present invention are made in accordance with the provisions of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure (the Budapest Treaty).

[0229] The authors contend that pursuant to 37 C.F.R. 1.808(a) (2) "All restrictions placed by the contributor on the availability to the public of the deposited material shall be irrevocably lifted upon the grant of the patent." This statement is subject to paragraph (b) of this section (i.e., 37 C.F.R. 1.808(b)).

[0230] Biologically pure cultures of Rahnella aquatilis and Enterobacter sacchari were deposited on July 14, 2015 with the American Type Culture Collection (ATCC; International Depositary Authority), 10801 University Blvd., Manassas, VA 20110, USA, and assigned ATTC Patent Deposit Designation PTA numbers -122293 and PTA-122294, respectively. Relevant deposit information is provided in Table A below.

[0231] Enterobacter sacchari is currently reclassified as Kosakonia sacchari , this name for this organism can be used interchangeably throughout the description.

[0232] Many of the microorganisms of the present invention are derived from two wild-type strains as shown in Figure 18 and Figure 19. Strain CI006 is a bacterial species previously classified in the genus Enterobacter (see above reclassification in Kosakonia ) and in Figure 19 shows a line of mutants that are derived from CI006. Strain CI019 is a species of bacteria classified in the genus Rahnella and Figure 19 shows the line of mutants that are derived from CI019. With respect to figures 18 and figures 19 , it should be noted that the strains containing CM in the name, are mutants of the strains depicted directly to the left of the specified strain of CM. Deposit information for CI006 Kosakonia wild type (WT) and CI019 Rahnella WT is shown in Table A below.

[0233] Some of the microorganisms described in this application were deposited on January 06, 2017 or August 11, 2017 at the Bigelow National Center for Marine Algae and Microbiota (NCMA), located at 60 Bigelow Drive, East Boothbay, Maine 04544, USA. As stated above, all deposits are made in accordance with the terms of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure. Bigelow National Center for Marine Algae and Microbiota access numbers and deposit dates for the above Budapest Treaty deposits are shown in Table A.

[0234] Biologically pure cultures of Kosakonia sacchari (WT), Rahnella aquatilis (WT), and a variant strain of Kosakonia sacchari were deposited on January 06, 2017 at the Bigelow National Center for Marine Algae and Microbiota (NCMA), located at 60 Bigelow Drive, East Boothbay, Maine 04544, USA, and have been assigned NCMA Patent Deposit Designation numbers 201701001, 201701003, and 201701002, respectively. Relevant deposit information is provided in Table A below.

[0235] Biologically pure cultures of variant strains of Kosakonia sacchari were deposited on August 11, 2017 at the Bigelow National Center for Marine Algae and Microbiota (NCMA), located at 60 Bigelow Drive, East Boothbay, Maine 04544, USA, and assigned NCMA Patent Deposit numbers Designation 201708004, 201708003 and 201708002, respectively. Relevant deposit information is provided in Table A below.

[0236] A biologically pure culture of Klebsiella variicola (WT) was deposited on August 11, 2017 at the Bigelow National Center for Marine Algae and Microbiota (NCMA), located at 60 Bigelow Drive, East Boothbay, Maine 04544, USA, and has been assigned an NCMA Patent number Deposit Designation 201708001 Biologically pure cultures of two variants of Klebsiella variicola were deposited on December 20, 2017 at the Bigelow National Center for Marine Algae and Microbiota (NCMA) located at 60 Bigelow Drive, East Boothbay, Maine 04544, USA and assigned NCMA Patent numbers Deposit Designation 201712001 and 201712002, respectively. Relevant deposit information is provided in Table A below.

Table A: Microorganisms deposited under the Budapest Treaty

Depository Main strain designation (some strains have multiple designations) Taxonomy Access number Deposit date ATCC Rahnella aquatilis PTA-122293 July 14, 2015 ATCC Enterobacter sacchari (taxonomically reclassified as Kosakonia sacchari after deposit) PTA-122294 July 14, 2015 NCMA CI006, PBC6.1, 6 Kosakonia sacchari (WT) 201701001 January 06, 2017 NCMA CI019, 19 Rahnella aquatilis (WT) 201701003 January 06, 2017 NCMA CM029, 6-412 Kosakonia sacchari 201701002 January 06, 2017 NCMA 6-403CM037 Kosakonia sacchari 201708004 August 11, 2017 NCMA 6-404, CM ³ 8, PBC6.38 Kosakonia sacchari 201708003 August 11, 2017 NCMA CM094, 6-881, PBC6.94 Kosakonia sacchari 201708002 August 11, 2017 NCMA CI137, 137, PB137 Klebsiella variicola (WT) 201708001 August 11, 2017 NCMA 137-1034 Klebsiella variicola 201712001 December 20, 2017 NCMA 137-1036 Klebsiella variicola 201712002 December 20, 2017

Isolated and biologically pure microorganisms

[0237] The present invention in certain embodiments relates to isolated and biologically pure microorganisms, which, among other things, have applications in agriculture. The microorganisms of the invention can be used in their isolated and biologically pure states, as well as formulated in a composition (for a description of illustrative compositions, see the section below). In addition, the invention provides compositions of microorganisms containing at least two representatives of the described isolated and biologically pure microorganisms, as well as methods for using these compositions of microorganisms. In addition, the invention provides methods for modulating nitrogen fixation in plants through the use of the described isolated and biologically pure microorganisms.

[0238] In certain aspects, the isolated and biologically pure microorganisms of the invention are those of Table A. In other aspects, isolated and biologically pure microorganisms according to the invention are obtained from microorganisms from table A. For example, provided herein is a strain, progeny, mutant, or derivative of a microorganism from Table A. The invention contemplates all possible combinations of microorganisms listed in Table A , where said combinations sometimes form consortia of microorganisms. Microorganisms from table A , individually or in any combination, can be combined with any plant, active substance (synthetic, organic, etc.), adjuvant, carrier, additives or biological component specified in the description.

Compositions

[0239] Compositions containing bacteria or populations of bacteria obtained by the methods described herein and/or having the characteristics as described herein may be in the form of a liquid, foam or dry product. Compositions containing bacteria or populations of bacteria obtained by the methods described herein and/or having the characteristics as described herein can also be used to improve the properties of plants. In certain examples, the composition containing populations of bacteria may be in the form of a dry powder, a slurry of powder and water, or a liquid seed treatment. Compositions containing populations of bacteria can be applied to the surface of the seeds, and it can be in liquid form.

[0240] The composition can be produced in bioreactors such as continuously stirred tank reactors, batch reactors, and on a farm. In certain examples, the compositions may be stored in a container such as a canister or mini-volume. In certain examples, the compositions may be stored in any item selected from the group consisting of a bottle, flask, ampoule, package, vessel, bag, box, crate, wrapper, carton, container, accumulator, shipping container, truck bed, and/ or body.

[0241] the Compositions can also be used to improve the properties of plants. In certain examples, one or more compositions can be applied to seeds. In certain examples, one or more compositions can be applied to the seedling. In certain examples, one or more compositions can be applied to the surface of the seeds. In certain examples, one or more compositions can be applied as a layer on the surface of the seeds. In certain examples, the composition that is applied to the seed may be in liquid form, in dry product form, in foam form, in the form of a slurry of powder and water, or in liquid seed treatment. In certain examples, one or more compositions can be applied to seeds and/or seedlings by spraying, dipping, coating, encapsulating and/or pollinating the seeds and/or seedlings with one or more compositions. In certain examples, several bacteria or populations of bacteria can be applied to seeds and/or seedlings of plants. In certain examples, at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, at least ten, or more than ten bacteria combinations of bacteria from one of the following genera: Acidovorax , Agrobacterium , Bacillus , Burkholderia , Chryseobacterium , Curtobacterium , Enterobacter , Escherichia , Methylobacterium , Paenibacillus , Pantoea , Pseudomonas , Ralstonia , Saccharibacillus , Sphingomonas , and Stenotrophomo nas .

[0242] In certain examples, at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, at least ten or more than ten bacteria and bacterial populations of an endophytic combination are selected from one of the following families: Bacillaceae , Burkholderiaceae , Comamonadaceae , Enterobacteriaceae , Flavobacteriaceae , Methylobacteriaceae , Microbacteriaceae , Paenibacillileae , Pseudomonnaceae , Rhizobiaceae , Sphingomonadaceae , Xanthomonadaceae , Cladosporiaceae , Gnom oniaceae , Incertae sedis , Lasiosphaeriaceae , Netriaceae and Pleosporaceae .

[0243] In certain examples, at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, at least ten or more than ten bacteria and bacterial populations of an endophytic combination are selected from one of the following families: Bacillaceae , Burkholderiaceae , Comamonadaceae , Enterobacteriaceae , Flavobacteriaceae , Methylobacteriaceae , Microbacteriaceae , Paenibacillileae , Pseudomonnaceae , Rhizobiaceae , Sphingomonadaceae , Xanthomonadaceae , Cladosporiaceae , Gononi aceae , Incertae sedis , Lasiosphaeriaceae , Netriaceae , Pleosporaceae .

[0244] Examples of compositions may include seed coatings for commercially important crops, such as sorghum, canola, tomato, strawberry, barley, rice, corn, and wheat. Examples of compositions may also include seed coatings for corn, soybeans, canola, sorghum, potatoes, rice, vegetables, cereals and oilseeds. Seeds, as provided herein, may be genetically modified organisms (GMO), non-GMO, organic forms, or conventional forms. In certain examples, the compositions can be sprayed onto the aerial parts of the plants or applied to the roots by placing them in the furrows in which the plant seeds are planted, watering the soil, or dipping the roots into a suspension of the composition. In certain examples, the compositions can be dehydrated in a suitable manner that retains cell viability and the ability to artificially populate and colonize host plants. The bacterial species may be present in the compositions at a concentration of from 10 ⁸ to 10 ¹⁰ cfu/ml. In certain examples, the compositions can be supplemented with trace metal ions such as molybdenum ions, iron ions, manganese ions, or combinations of these ions. Ion concentrations in the exemplary compositions described herein can be from about 0.1 mM to about 50 mM. Certain exemplary compositions may also be formulated with a carrier such as beta-glucan, carboxymethylcellulose (CMC), bacterial extracellular polymeric substance (EPS), sugar, animal milk, or other suitable carriers. In certain examples, peat or planting materials or biopolymers can be used as a carrier, in which the composition is placed in a biopolymer. Compositions containing the bacterial populations described herein can improve plant traits, such as promoting plant growth, maintaining high leaf chlorophyll content, increasing fruit or seed numbers, and increasing single fruit or seed weight.

[0245] Compositions containing the bacterial populations described herein can be applied to the surface of seeds. Thus, compositions are contemplated containing seeds coated with one or more of the bacterial species described herein. The seed coat can be formulated by mixing a population of bacteria with a porous, chemically inert granular carrier. Alternatively, the compositions can be applied directly to the furrows in which the seeds are planted, or sprayed onto the leaves of the plants, or applied by dipping the roots into a suspension of the composition. An effective amount of the composition can be used to fill an area of soil near the roots of a plant with growth of viable bacteria, or to colonize the leaves of a plant with growth of viable bacteria. Generally, an effective amount is an amount sufficient to produce plants with improved traits (eg, the desired level of nitrogen fixation).

[0246] The bacterial compositions described herein can be formulated using an agriculturally acceptable carrier. A formulation suitable for these embodiments may comprise at least one member selected from the group consisting of tackifying agent, microorganism stabilizer, fungicide, antibacterial agent, preservative, stabilizer, surfactant, anti-complex agent, herbicide, nematicide, an insecticide, a plant growth regulator, a fertilizer, a rodenticide, a humectant, a bactericide, a nutrient, or any combination thereof. In certain examples, the compositions may be suitable for long-term storage. For example, any of the compositions described herein may contain an agriculturally acceptable carrier (e.g., one or more of fertilizers, such as a non-natural fertilizer, tackifiers, such as a non-natural tackifier, and pesticides, such as a non-natural pesticide ). The non-natural tackifier may be, for example, a polymer, copolymer or synthetic wax. For example, any of the coated seeds, seedlings or plants described herein may contain such an agriculturally acceptable carrier in the seed coating. In any of the compositions or methods described herein, the agriculturally acceptable carrier may be or may contain a non-natural compound (eg, a non-natural fertilizer, a non-natural tackifier such as a polymer, copolymer, or synthetic wax, or a non-natural pesticide). Non-limiting examples of agriculturally acceptable carriers are described below. Additional examples of agriculturally acceptable carriers are known in the art.

[0247] In certain cases, the bacteria are mixed with an agriculturally acceptable carrier. The carrier may be a solid carrier or a liquid carrier, and may be in various forms, including microspheres, powders, emulsions, and the like. The carrier may be any one or more of a number of carriers that provide a number of properties such as increased stability, wettability, or dispersibility. Wetting agents such as natural or synthetic surfactants, which may be non-ionic or ionic surfactants, or a combination thereof, may be included in the compositions. Also, water-in-oil emulsions can be used to formulate compositions that contain isolated bacteria (see, for example, US Pat. No. 7,485,451). Suitable formulations that can be prepared include wettable powders, granules, gels, agarose plates or beads, thickeners and the like, microencapsulated particles and the like, liquids such as aqueous liquids, aqueous suspensions, water-in-emulsions oil, etc. The composition may contain grains or legumes, for example, ground grains or beans, broth or fine flour obtained from grains or beans, starch, sugar or oil.

[0248] In certain embodiments, the implementation of the agricultural carrier may be a soil or environment for growing plants. Other agricultural vehicles that can be used include water, fertilizers, vegetable oils, humectants, or combinations thereof. Alternatively, the agricultural carrier may be a solid such as diatomaceous earth, clay, silica, alginate, clay soil, bentonite, vermiculite, seed pods, other plant and animal products, or combinations thereof, including granules, sediments, or suspensions. Mixtures of any of the above ingredients are also contemplated as non-limiting examples, such as pestle (fine flour and kaolin clay), agar balls or fine flour in clay, sand or clay soil, etc. The formulations may contain food sources for bacteria such as barley, rice or other biological materials such as seeds, plant parts, sugar cane pomace, grain husks or stalks, shredded plant material or wood from construction site waste, sawdust or fine fibers. after processing paper, fabric or wood.

[0249] For example, a fertilizer can be used to help promote growth or provide nutrients to seeds, seedlings, or plants. Non-limiting examples of fertilizers include nitrogen, phosphorus, potassium, calcium, sulfur, magnesium, boron, chloride, manganese, iron, zinc, copper, molybdenum, and selenium (or salts thereof). Additional examples of fertilizers include one or more amino acids, salts, carbohydrates, vitamins, glucose, NaCl, yeast extract, NH ₄ H ₂ PO ₄ , (NH ₄ ) ₂ SO ₄ , glycerol, valine, L-leucine, lactic acid, propionic acid, succinic acid, malic acid, citric acid, KH tartrate, xylose, lyxose and lecithin. In one embodiment, the formulation may contain a tackifier or adhesive (referred to as an adhesive) to help bind other active agents to the substance (eg, the surface of the seeds). Such agents are suitable for combination of bacteria with carriers which may contain other compounds (eg control agents which are non-biological) to form a coating composition. Such compositions help to provide coatings around a plant or seed while maintaining contact of microorganisms and other agents with the plant or plant part. In one embodiment, the adhesives are selected from the group consisting of: alginate, gums, various starches, lecithins, formononetin, polyvinyl alcohol, alkaline formononetinate, hesperetin, polyvinyl acetate, cephalins, gum arabic, xanthan gum, mineral oil, polyethylene glycol (PEG), polyvinylpyrrolidone (PVP), arabino-galaktana, methyl cellulose, PEG 400, chitosan, polyacrylamide, polyacrylate, aoliacrilonitril, glycerin, tretilenlycol, vinylcetate, gelllan gummy, polyvinyl, polyvinyl, carboxymethyl cellulose, gkhatti and polyoxyati and polyoxyati and polyoxyati and polyoxyati and polyoxyati and polyoxyati and polyoxyeti Len-polyoxibulent block-polymers.

[0250] In certain embodiments, the adhesives may be, for example, a wax such as carnauba wax, beeswax, Chinese wax, shellac wax, spermaceti wax, candelilla wax, hydrogenated castor oil, oricuria wax, and rice bran wax, a polysaccharide (e.g. , starch, dextrins, maltodextrins, alginate and chitosans), fat, oil, protein (eg gelatin and zeins), gum arabic and shellacs. Adhesives may be non-natural compounds such as polymers, copolymers and waxes. For example, non-limiting examples of polymers that can be used as an adhesive include: polyvinyl acetates, polyvinyl acetate copolymers, ethylene-vinyl acetate (EVA) copolymers, polyvinyl alcohols, polyvinyl alcohol copolymers, cellulose compounds (e.g. various types of ethylcellulose, methylcellulose, hydroxymethylcellulose, hydroxypropylcellulose and carboxymethylcelluloses), polyvinylpyrrolidones, vinyl chloride, vinylidene chloride copolymers, calcium lignosulfonates, acrylic copolymers, polyvinyl acrylates, polyethylene oxide, acylamide polymers and copolymers, polyhydroxyethyl acrylate, monomeric methylacrylamides and polychloroprene.

[0251] In certain examples, one or more of the adhesives, antifungals, growth regulators, and pesticides (eg, insecticides) are non-natural compounds (eg, in any combination). Additional examples of agriculturally acceptable carriers include dispersants (eg, polyvinylpyrrolidone/vinyl acetate PVPIVA S-630), surfactants, binders, and fillers.

[0252] The composition may also contain a surfactant. Non-limiting examples of surfactants include mixtures of nitrogen fertilizers and surfactants such as Prefer 28 (Cenex), Surf-N (US), Inhance (Brandt), P-28 (Wilfarm) and Patrol (Helena); esterified seed oils include Sun-It II (AmCy), MSO (UAP), Scoil (Agsco), Hasten (Wilfarm) and Mes-100 (Drexel); and organo-silicone surfactants include Silwet L77 (UAP), Silikin (Terra), Dyne-Amic (Helena), Kinetic (Helena), Sylgard 309 (Wilbur-Ellis) and Century (Precision). In one embodiment, the surfactant is present at a concentration of 0.01% v/v. up to 10% v/v In another embodiment, the surfactant is present at a concentration of 0.1% v/v. up to 1% v/v

[0253] In certain cases, the composition contains a microorganism stabilizer. Such agent may include a desiccant, which may include any compound or mixture of compounds that can be classified as a desiccant, whether or not the compound or compounds are used at such concentrations that they actually exhibit a drying effect on the liquid inoculating agent. Such desiccants are ideally compatible with the bacterial populations used and should maintain the ability of the microbial population to survive seed application and survive dehydration. Examples of suitable moisture scavengers include one or more of trehalose, sucrose, glycerol and methylene glycol. Other suitable moisture scavengers include non-limiting examples of non-reducing sugars and sugar alcohols (eg mannitol or sorbitol). The amount of desiccant incorporated into the formulation may range from about 5% to about 50% by weight/volume, for example, from about 10% to about 40%, from about 15% to about 35%, or from about 20% to approximately 30%. In certain instances, it is preferred that the composition contains agents such as a fungicide, antibacterial agent, herbicide, nematocide, insecticide, plant growth regulator, rodenticide, bactericide or nutrient. In certain examples, the agents may include protective agents that provide protection against pathogens on the surface of the seeds. In certain instances, protective agents may provide some level of control of soil pathogens. In certain instances, the protectants may be effective predominantly on the surface of the seed.

[0254] In certain examples, the fungicide may include a compound or a chemical or biological agent that can inhibit or kill the fungus. In certain examples, the fungicide may include compounds that may be fungistatic or fungicidal. In certain instances, the fungicide may be a preservative or agents that are effective primarily on the surface of the seed, providing protection against pathogens on the surface of the seed and providing some level of control of soil pathogens. Non-limiting examples of protective fungicides include captan, maneb, thiram, or fludioxonil.

[0255] In certain examples, the fungicide may be a systemic fungicide that can be absorbed into the growing seedling and inhibit or kill the fungus within host plant tissues. Systemic fungicides used for seed treatment include, but are not limited to, the following: azoxystrobin, carboxin, mefenoxam, metalaxyl, thiabendazole, trifloxystrobin, and various triazole fungicides including difenoconazole, ipconazole, tebuconazole, and triticonazole. Mefenoxam and metalaxyl are predominantly used to attack Pythium and Phytophthora water molds. Certain fungicides are preferred over others depending on the plant species due to minor differences in susceptibility of pathogenic fungal species or due to differences in fungicide distribution or plant susceptibility. In certain instances, the fungicide may be a biological control such as a bacterium or fungus. Such organisms may be parasites of pathogenic fungi or secrete toxins or other substances that can destroy or otherwise prevent the growth of fungi. Any type of fungicide can be used as a control agent in a seed formulation, especially fungicides that are widely used in plants.

[0256] In certain examples, the seed coating composition contains a control agent that has antibacterial properties. In one embodiment, the antibacterial control agent is selected from the compounds described elsewhere herein. In another embodiment, the compound is streptomycin, oxytetracycline, oxolinic acid, or gentamicin. Other examples of antibacterial compounds that can be used as part of a seed coating composition include compounds based on dichlorophene and benzyl alcohol hemiformal (Proxel® available from ICI or Acticide® RS available from Thor Chemie and Kathon® MK 25 available from Rohm & Haas ) and isothiazolinone derivatives such as alkylisothiazolinones and benzisothiazolinones (Acticide® MBS manufactured by Thor Chemie).

[0257] In certain examples, the growth regulator is selected from the group consisting of: abscisic acid, amidochlor, ancymidol, 6-benzylaminopurine, brassinolide, butralin, chlormequat (chlormequat chloride), choline chloride, cyclanilide, daminoside, dikegulac, dimethypine, 2,6- dimethylpuridine, ethephon, flumetralin, flurprimidol, fluthiacet, forchlorfenuron, gibberellic acid, inabenfide, indole-3-acetic acid, maleic hydrazide, mefluidide, mepiquat (mepiquat chloride), naphthaleneacetic acid, N-6-benzyladenine, paclobutrazole, phosphotriti prohexadione oate, 2 ,3,5-tri-iodobenzoic acid, trinexapacethyl and uniconazole. Additional non-limiting examples of growth regulators include brassinosteroids, cytokinins (eg, kinetin and zeatin), auxins (eg, indoleacetic acid and indole acetylaspartate), flavonoids and isoflavonoids (eg, formononetin and diosmetin), phytoauxins (eg, glyceollin), and phytoalexin-inducible oligosaccharides (eg. , pectin, chitin, chitosan, polygalacturonic acid and oligogalacturonic acid) and gibellerins. Such agents are ideally compatible with the agricultural seed or seedling to which the composition is applied (eg, it must not be harmful to plant growth or health). In addition, ideally, the agent is an agent that does not pose a safety hazard to human, animal, or industrial use (e.g., no safety hazards, or the compound is sufficiently unstable such that a consumable herbal product derived from the plant contains negligible amounts of it). connections).

[0258] Certain examples of antagonistic nematode biocontrol agents include ARF18; 30 species of Arthrobotrys ; Chaetomium species; species of Cylindrocarpon ; Exophilia species; Fusarium species; species of Gliocladium ; Hirsutella species; Lecanicillium species; species of Monacrosporium ; Myrothecium species; Neocosmospora species; Paecilomyces species; species of Pochonia ; species of Stagonospora ; vesicular-arbuscular mycorrhizal fungi , Burkholderia species; Pasteuria spp., Brevibacillus spp.; Pseudomonas and Rhizobacteria species. Particularly preferred nematode antagonist biocontrol agents include ARF18, Arthrobotrys oligospora , Arthrobotrys dactyloides , Chaetomium globosum , Cylindrocarpon heteronema , Exophilia jeanselmei , Exophilia pisciphila , Fusarium aspergilus , Fusarium solani , Gclaliodium catenulatum , Gliocladium roseum , Gliocladium vixens , Hirsutella rhossiliensis , Hirsutella minnesotensis , Lecanicillium lecanii , Monacrosporium drechsleri , Monacrosporium gephyropagum , Myrotehcium verrucaria , Neocosmospora vasinfecta , Paecilomyces lilacinus , Pochonia chlamydosporia , Stagonospora heteroderae , Stagonospora phaseoli , vesicular-arbuscular mycorrhizal fungi , Burkholderia ce pacia , Pasteuria penetrans , Pasteuria thornei , Pasteuria nishizawae , Pasteuria ramosa , Pastrueia usage , strain G4 Brevibacillus laterosporus , Pseudomonas fluorescens and Rhizobacteria .

[0259] Specific examples of nutrients can be selected from the group consisting of nitrogen fertilizers, including, but not limited to, urea, ammonium nitrate, ammonium sulfate, non-pressure nitrogen solutions, aqueous ammonia, anhydrous ammonia, ammonium thiosulfate, sulfur-coated urea, urea-formaldehydes , IBDU, polymer-coated urea, calcium nitrate, ureaform and methylene urea, phosphate fertilizers such as ammonium diphosphate, ammonium monophosphate, ammonium polyphosphate, concentrated superphosphate and triple superphosphate, and potash fertilizers such as potassium chloride, potassium sulfate, potassium magnesium sulfate , potassium nitrate. Such compositions in the seed coating composition may be in the form of free salts or ions. Alternatively, the nutrients/fertilizers can be complexed or chelated to provide sustained release over an extended period.

[0260] Specific examples of rodenticides can be selected from the group consisting of 2-isovalerylindan-1,3-dione, 4-(quinoxalin-2-ylamino)benzenesulfonamide, alpha-chlorohydrin, aluminum phosphide, alpha-naphthylthiourea, arsenic oxide, carbonate barium, bistiosemi, brodifacoum, bromadiolone, bromethalin, calcium cyanide, chloralose, chlorophacinone, cholecalciferol, cumachlor, coumafuril, coumatetralyl, crimidine, difenacum, difetialon, difacinone, ergocalciferol, flocumafen, fluoroacetamide, flupropadine, hydrochloride f lupropadine, hydrogen cyanide, iodomethane, lindane , magnesium phosphide, methyl bromide, norbormide, fosacetim, phosphine, phosphorus, pindone, potassium arsenite, pyrinuron, skylyroside, sodium arsenite, sodium cyanide, sodium fluoroacetate, strychnine, thallium sulfate, warfarin, and zinc phosphide.

[0261] In liquid form, for example, in solution or suspension, bacterial populations can be mixed or suspended in water or in aqueous solutions. Suitable liquid diluents or carriers include water, aqueous solutions, petroleum distillates, or other liquid carriers.

[0262] Solid compositions can be prepared by dispersing bacterial populations in and on a suitably pulverized solid carrier such as peat, wheat, bran, vermiculite, clay, talc, bentonite, diatomaceous earth, fuller's clay, pasteurized soil, and the like. When such formulations are used as wettable powders, biocompatible dispersants such as non-ionic, anionic, amphoteric or cationic dispersants and emulsifiers can be used.

[0263] Solid carriers used in the formulation include, for example, mineral carriers such as kaolin clay, pyrophyllite, bentonite, montmorillonite, diatomaceous earth, acid white earth, vermiculite, and perlite, and inorganic salts such as ammonium sulfate, ammonium phosphate, ammonium nitrate, urea, ammonium chloride and calcium carbonate. Organic fine powders such as wheat flour, wheat bran and rice bran can also be used. Liquid carriers include vegetable oils such as soybean oil and cottonseed oil, glycerin, ethylene glycol, polyethylene glycol, propylene glycol, polypropylene glycol, and the like.

Application of bacterial populations for crops

[0264] The bacterial composition or population of bacteria described herein can be applied to the furrow, in the form of talc, or as a seed coat. The composition can be applied to the package of seeds in general, mini-volume, in a bag or in talc.

[0265] The farmer can plant the treated seeds and grow the crop in conventional, two-row, or no-till methods. Seed can be distributed using the control hopper or a separate hopper. Seeds can also be distributed using compressed air or manually. Seeding of seeds can be carried out using technologies with variable speed. In addition, using technologies with variable speed can carry out the application of bacteria or populations of bacteria described in this document. In certain examples, the bacteria can be applied to corn, soybean, canola, sorghum, potato, rice, vegetable, cereal, pseudocereal and oilseed seeds. Examples of cereals may include barley, fonio, oats, palmer grass, rye, pearl millet, sorghum, spelt, teff, triticale and wheat. Examples of pseudocereals may include walnut, buckwheat, horsetail, chia, flax, grain amaranth, ganza, quinoa, and sesame. In certain instances, the seeds may be genetically modified organisms (GMO), non-GMO, organic, or conventional.

[0266] In addition to crop treatment, additives such as microfertilizer, PGR, herbicide, insecticide, and fungicide can be used. Examples of additives include crop protectants such as insecticides, nematocides, fungicides, strengthening agents such as dyes, polymers, pelletizing, priming, and disinfectants, and other agents such as an inoculant, PGR, softener, and micronutrients. PGRs can be natural or synthetic plant hormones that affect root growth, flowering, or stem elongation. PGRs may include auxins, gibberellins, cytokines, ethylene, and abscisic acid (ABA).

[0267] The composition can be applied to the furrow in combination with a liquid fertilizer. In certain instances, the liquid fertilizer may be contained in tanks. NPK fertilizers contain macronutrients with sodium, phosphorus and potassium.

[0268] The composition can improve the properties of plants, for example, promoting plant growth, maintaining a high chlorophyll content in leaves, increasing the number of fruits or seeds, and increasing the weight of single fruits or seeds. The methods of the present invention can be used to add or improve one or more of a number of desired properties. Examples of properties that can be added or improved include: root biomass, root length, height, shoot height, number of leaves, water use efficiency, total biomass, yield, fruit size, grain size, photosynthesis rate, drought tolerance, heat tolerance, salt tolerance, stress tolerance low nitrogen, nitrogen utilization efficiency, nematode resistance, fungal pathogen resistance, bacterial pathogen resistance, viral pathogen resistance, metabolite level, metabolite level modulation, proteome expression. Required properties, including height, total biomass, root and/or shoot biomass, seed germination, seedling viability, photosynthesis efficiency, transpiration rate, number or weight of seeds/fruits, grain or fruit yield per plant, leaf chlorophyll content, photosynthesis rate, root length, or any combination thereof, can be used to determine growth and compared with the growth rate of reference crop plants (eg, plants without addition and/or enhancement) grown under identical conditions. In certain examples, desired properties, including height, total biomass, root and/or shoot biomass, seed germination, seedling viability, photosynthesis efficiency, transpiration rate, number or weight of seeds/fruits, grain or fruit yield per plant, chlorophyll content in leaves, photosynthesis rate, root length, or any combination thereof can be used to determine growth and compare with the growth rate of a reference crop plant (eg, a plant without addition and/or enhancement) grown under similar conditions.

[0269] Agronomic traits of the host plant may include, but are not limited to, the following: altered oil content, altered protein content, altered seed carbohydrate composition, altered seed oil composition and altered seed protein composition, chemical resistance, cold tolerance, delayed aging, resistance disease resistance, drought tolerance, ear weight, improved growth, improved viability, heat tolerance, herbicide tolerance, herbivore tolerance, improved nitrogen fixation, improved nitrogen utilization, improved root architecture, improved water use efficiency, increased biomass, increased root length , increased seed weight, increased shoot growth, increased yield, increased yield under water limited conditions, grain weight, grain moisture content, metal tolerance, number of cobs, number of kernels per cob, number of pods, improved nutrition, resistance to pathogenic resistance to pests, improved photosynthetic capacity, salinity tolerance, green color retention, improved vigor, increased dry weight of mature seeds, increased wet weight of mature seeds, increased number of mature seeds per plant, increased chlorophyll content, increased number of pods per plant , increased pod length per plant, reduced wilted leaves per plant, reduced severe wilted leaves per plant, and increased non-withered leaves per plant, detectable change in metabolite level, detectable change in transcript level, and detectable change in proteome compared to isoline plant grown from seeds without the specified composition of the preparation for seed treatment.

[0270] In certain instances, plants are inoculated with bacteria or populations of bacteria that are isolated from the same plant species as a constituent of the inoculated plant. For example, a bacterium or population of bacteria that is normally found in one cultivar of Zea mays (maize) is associated with a plant constituent of another cultivar of Zea mays that is deficient in said bacteria and bacterial populations in its natural state. In one embodiment, the bacteria and bacterial populations are obtained from a plant of a related species as a constituent of the inoculated plant. For example, bacteria and bacterial populations that are commonly found in Zea diploperennis , Iltis et al., (Diploperennial teosinte) are used for Zea mays (maize) or vice versa. In certain instances, plants are inoculated with bacteria and bacterial populations that are heterologous with respect to the plant element of the inoculated plant. In one embodiment, the bacteria and bacterial populations are obtained from a plant of another species. For example, bacteria and bacterial populations that are commonly found in dicotyledonous plants are applied to monocotyledonous plants (eg, corn is inoculated with bacteria and bacterial populations derived from soybeans) or vice versa. In other cases, the bacteria and bacterial populations to be inoculated into the plant are obtained from related species of the plant being inoculated. In one embodiment, the bacteria and bacterial populations are obtained from a related taxon, such as a related species. The plant of another species may be an agricultural plant. In another embodiment, the bacteria and bacterial populations are part of the designed composition, inoculated into any element of the host plant.

[0271] In certain examples, the bacteria or bacterial population is exogenous, where the bacteria and bacterial population are isolated from a plant other than the plant being inoculated. For example, in one embodiment, the bacteria or population of bacteria can be isolated from another plant of the same species as the plant being inoculated. In certain cases, the bacteria or population of bacteria can be isolated from species related to the inoculated plant.

[0272] In certain examples, the bacteria and bacterial populations described herein are capable of moving from one type of tissue to another. For example, detection and isolation of the bacteria and bacterial populations of the present invention in mature plant tissues after coating the outer surface of the seed demonstrates their ability to migrate from the outside of the seed into the vegetative tissues of the mature plant. Thus, in one embodiment, the bacterial population and bacterial populations are capable of moving from outside the seed into the vegetative tissues of the plant. In one embodiment, the bacteria and bacterial populations that are applied to the seeds of a plant are capable of localizing to other tissues of the plant when the seed germinates into a vegetative state. For example, bacteria and populations of bacteria may be capable of localization in any of the tissues of a plant, including: root, accessory root, germinal root, root hair, shoot, leaf, flower, bud, panicle, meristem, pollen, pistil, ovary, stamen, fruit, underground shoot, root shoot, nodule, tuber, trichome, guard cells, hydathode, petal, sepal, grain coat, spike axis, vascular cambium, phloem and xylem. In one embodiment, the bacteria and bacterial populations are capable of localizing to the root and/or root hairs of a plant. In another embodiment, the bacteria and populations of bacteria are capable of localization in photosynthetic tissues, such as leaves and shoots of a plant. In other cases, bacteria and bacterial populations are localized in plant vascular tissues, such as xylem and phloem. In yet another embodiment, the bacteria and bacterial populations are capable of localization in the reproductive tissues (flower, pollen, pistil, ovary, stamen, fruit) of the plant. In another embodiment, the bacteria and bacterial populations are capable of localization in the root, shoots, leaves and reproductive tissues of a plant. In yet another embodiment, the bacteria and bacterial populations colonize the fruit or seed tissue of the plant. In yet another embodiment, the bacteria and bacterial populations are capable of colonizing the plant such that they reside on the surface of the plant (ie, their presence is detected on the outside of the plant or in the plant's episphere). In other embodiments, the bacteria and bacterial populations are capable of localizing to substantially the entire plant or all tissues of the plant. In certain embodiments, the bacteria and bacterial populations are not located in the root of the plant. In other cases, bacteria and bacterial populations are not localized in the photosynthetic tissues of the plant.

[0273] The effectiveness of the compositions can also be assessed by determining the relative maturity of the crop or crop heat units (CHU). For example, a population of bacteria can be applied to corn, and corn growth can be judged according to the relative maturity of the corn grain or the time it takes for the corn kernel to reach its maximum weight. Crop heat units (CHU) can also be used to predict maize maturation. CHU determine the amount of stored heat by determining the daily maximum temperatures during crop growth.

[0274] In examples, bacteria can be localized in any of the plant tissues, including: root, accessory root, germinal root, root hair, shoot, leaf, flower, bud, panicle, meristem, pollen, pistil, ovary, stamen, fruit , underground shoot, root shoot, nodule, tuber, trichome, guard cells, hydathode, petal, sepal, grain coat, spike axis, vascular cambium, phloem and xylem. In another embodiment, the bacterium or population of bacteria is capable of localization in photosynthetic tissues, such as leaves and shoots of a plant. In other cases, bacteria and bacterial populations are localized in the vascular tissues of the plant, such as the xylem and phloem. In another embodiment, the bacterium or population of bacteria is capable of localization in the reproductive tissues (flower, pollen, pistil, ovary, stamen, or fruit) of the plant. In another embodiment, the bacteria and bacterial populations are capable of localization in the root, shoots, leaves and reproductive tissues of a plant. In another embodiment, the bacteria or population of bacteria colonize the fruit or seed tissue of the plant. In yet another embodiment, the bacteria or population of bacteria are capable of colonizing the plant such that they reside on the surface of the plant. In another embodiment, the bacteria, or population of bacteria, is capable of localizing to substantially the entire plant or all tissues of the plant. In certain embodiments, the bacteria or population of bacteria is not located in the root of the plant. In other cases, bacteria and bacterial populations are not localized in the photosynthetic tissues of the plant.

[0275] The effectiveness of bacterial compositions applied to crops can be assessed by determining various crop growth properties, including, but not limited to, seeding rate, planting power, root strength, drought tolerance, plant height, dry weight, and test weight.

plant species

[0276] The methods and bacteria described herein are suitable for any of a variety of plants, such as plants of the genera Hordeum, Oryza, Zea, and Triticeae . Other non-limiting examples of suitable plants include mosses, lichens and algae. In some cases, plants are of economic, social and/or environmental importance as food crops, fiber crops, oilseeds, plants used in the forestry or pulp and paper industry, biofuel feedstock and/or ornamental plants. In certain examples, plants can be used to produce economically valuable products such as grains, fine flour, starch, syrup, wholemeal, oil, film, packaging, biological additives, pulp, animal feed, fish food, base material. for industrial chemicals, baked goods, processed human food, sugar, alcohol and/or protein. Non-limiting examples of crops include corn, rice, wheat, barley, sorghum, millet, oats, triticale rye, buckwheat, sweet corn, sugarcane, onions, tomatoes, strawberries, and asparagus.

[0277] In certain examples, plants that can be obtained or improved by the methods and compositions described herein may include plants that are important or of interest to agriculture, horticulture, biomass for the production of biofuel molecules and other chemicals, and/ or for forestry. Certain examples of these plants may include pineapple, banana, coconut, lilies, grass peas, and herbs; and dicotyledonous plants such as, for example, peas, alfalfa, physalis, melon, chickpeas, chicory, clover, cabbage, lentils, soybeans, tobacco, potatoes, sweet potatoes, radishes, kale, rapeseed, apple trees, grapes, cotton, sunflower, arabidopsis, canola, citrus fruits (including orange, tangerine, kumquat, lemon, lime, grapefruit, tangerine, tangelo, citron and pomelo), pepper, beans and lettuce, Panicum virgatum (brush), Sorghum bicolor (sorghum, Sudan), Miscanthus giganteus (miscanthus), Saccharum species (sugarcane), Populus balsamifera (poplar), Zea mays (maize), Glycine max (soybean), Brassica napus (canola), Triticum aestivum (wheat), Gossypium hirsutum (cotton), Oryza sativa (rice), Helianthus annuus (sunflower), Medicago sativa (alfalfa), Beta vulgaris (sugar beet), Pennisetum glaucum (American millet), Panicum spp. Sorghum spp ., Miscanthus spp ., Saccharum spp ., Erianthus spp ., Populus spp ., Secale cereale (rye), Salix spp. (willow), Eucalyptus spp . (eucalyptus), Triticosecale spp . (wheat - 25 wheat X rye), Bamboo , Carthamus tinctorius (safflower), Jatropha curcas (jatropha), Ricinus communis (castor bean), Elaeis guineensis ( oil palm), Phoenix dactylifera (date palm), Archontophoenix cunninghamiana (royal palm), Syagrus romanzoffiana (arecastrum), Linum usitatissimum (flax), Brassica juncea , Manihot esculenta (cassava), Lycopersicon esculentum (tomato), Lactuca saliva (lettuce) , Musa paradisiaca (banana), Solanum tuberosum (potato), Brassica oleracea (broccoli, cauliflower, Brussels sprouts), Camellia sinensis (tea), Fragaria ananassa (strawberry), Theobroma cacao (cocoa), Coffea arabica (coffee), Vitis vinifera (grape), Ananas comosus (pineapple), Capsicum annum (hot and sweet pepper), Allium cepa (onion), Cucumis melo (melon), Cucumis sativus (cucumber), Cucurbita maxima (pumpkin), Cucurbita moschata (pumpkin), Spinacea oleracea (spinach), Citrullus lanatus (watermelon), Abelmoschus esculentus (okra), Solanum melongena (eggplant), Papaver somniferum (opium poppy), Papaver orientale , Taxus baccata , Taxus brevifolia , Artemisia annua , Cannabis saliva , Camptotheca acuminate , Catharanthus roseus , Vinca rosea , Cinchona officinalis , Coichicum autumnale , Veratrum californica , Digitalis lanata , Digitalis purpurea , Dioscorea 5 spp., Andrographis paniculata , Atropa belladonna , Datura stomonium , Berberis spp., Cephalotaxus spp., Ephedra sinica , Ephedra spp., Erythroxy lum coca , Galanthus wornorii , Scopolia species , Lycopodium serratum ( Huperzia serrata ), Lycopodium species, Rauwolfia serpentina , Rauwolfia species, Sanguinaria canadensis , Hyoscyamus species, Calendula officinalis , Chrysanthemum parthenium , Coleus forskohlii , Tanacetum parthenium , Parthenium argentatum (guayule), species Hevea (rubber), Mentha spicata (mint), Mentha piperita (mint), Bixa orellana , Alstroemeria spp., Rosa spp. (rose), Dianthus caryophyllus (carnation), Petunia spp . (petunia), Poinsettia pulcherrima (poinsettia), Nicotiana tabacum (tobacco), Lupinus albus (lupine), Uniola paniculata (oats), Hordeum vulgare (barley) and Lolium species (rye).

[0278] In certain examples, a monocot plant can be used. Monocots belong to the orders Alismatales , Arales , Arecales , Bromeliales , Commelinales , Cyclanthales , Cyperales , Eriocaulales , Hydrocharitales , Juncales , Lilliales , Najadales , Orchidales , Pandanales , Poales , Restionales , Triuridales , Typhales , and Zingiberales . Plants belonging to the class Gymnospermae are Cycadales , Ginkgoales , Gnetales and Pinales . In certain examples, the monocot plant may be selected from the group consisting of corn, rice, wheat, barley, and sugarcane.

[0279] In certain examples, a dicot plant can be used, including plants belonging to the orders Aristochiales , Asterales , Batales , Campanulales , Capparales , Caryophyllales , Casuarinales , Celastrales , Cornales , Diapensales , Dilleniales , Dipsacales , Ebenales , Ericales , Eucomiales , Euphorbiales , Fabal es , Fagales , Gentianales , Geraniales , Haloragales , Hamamelidales , Middles , Juglandales , Lamiales , Laurales , Lecythidales , Leitneriales , Magniolales , Malvales , Myricales , Myrtales , Nymphaeales , Papeverales , Piperales , Plantaginales , Plumb aginales , Podostemales , Polemoniales , Polygalales , Polygonales , Primulales , Proteales , Rafflesiales , Ranunculales , Rhamnales , Rosales , Rubiales , Salicales , Santales , Sapindales , Sarraceniaceae , Scrophulariales , Theales , Trochodendrales , Umbellales , Urticales and Violates . In certain instances, the dicot may be selected from the group consisting of cotton, soy, pepper, and tomato.

[0280] In some cases, the improved plant is difficult to experimental conditions. For example, it may take too long for a crop to grow sufficiently to evaluate an improved trait almost consistently across multiple iterations. Thus, the first plant from which the bacteria are initially isolated and/or the plurality of plants to which the genetically modified bacteria are used may be model plants, such as a plant more amenable to analysis under the required conditions. Non-limiting examples of model plants include Setaria , Brachypodium and Arabidopsis . The ability of bacteria isolated by the method of the present invention using a model plant can then be applied to another type of plant (eg crop) to confirm the acquisition of an improved trait.

[0281] Traits that can be improved by the methods described herein include any observable characteristics of a plant, including, for example, growth rate, growth, weight, color, taste, smell, changes in the plant's production of one or more compounds (including, for example, metabolites , proteins, drugs, carbohydrates, oils and any other compounds). Also contemplated is selection of plants based on genotypic information (eg, including a plant's gene expression profile in response to bacteria or identification of the presence of genetic markers, such as genetic markers associated with increased nitrogen fixation). Plants, as opposed to the presence of a particular property or trait (such as a desired property or trait), can also be selected based on the absence, suppression, or inhibition of a particular property or trait (such as an undesirable property or trait).

Concentrations and application levels of agricultural compositions

[0282] As indicated above, the agricultural compositions of the present invention, which contain these microorganisms, can be applied to plants in a variety of ways. In two specific aspects of the invention, a furrow treatment or seed treatment is provided.

[0283] For seed treatment embodiments, the microorganisms of the invention can be found on seeds at a variety of concentrations. For example, microorganisms in seed treatment may be present at a CFU concentration per seed: 1×10 ¹ , 1×10 ² , 1×10 ³ , 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ . 1×10 ⁸ , 1×10 ⁹ , 1×10 ¹⁰ or more. In specific aspects, the seed treatment compositions contain from about 1×10 ⁴ to about 1×10 ⁸ CFU per seed. In other specific aspects, the seed treatment composition contains from about 1×10 ⁵ to about 1×10 ⁷ CFU per seed. In other aspects, the seed impregnation composition contains approximately 1×10 ⁶ cfu per seed.

[0284] In the United States, approximately 10% of the corn area is planted at a seed density greater than approximately 36,000 seeds per acre; 1/3 of the corn area planted at a seed density of approximately 33,000 to 36,000 seeds per acre; 1/3 of the area under corn is planted at a seed density of approximately 30,000 to 33,000 seeds per acre, and the remainder of the area is variable. See, "Corn Seeding Rate Considerations" by Steve Butzen, available at https://www.pioneer.com/home/site/us/agronomy/library/corn-seeding-rate-considerations/

[0285] Table B below uses various CFU concentrations per seed in the envisaged seed treatment embodiment (cross rows) and various acreage planting densities (1 column: 15K-41K) to calculate the total CFU per acre that can be used in different agricultural conditions (i.e. seed treatment concentration per seed × seed planting density per acre). Thus, if a seed treatment with 1×10 ⁶ CFU per seed is used and 30,000 seeds per acre are planted, the total CFU per acre will be 3×10 ¹⁰ (ie 30K×1×10 ⁶ ).

Table B : Calculation of total CFU per acre for seed treatment options

Corn population (i.e. seeds per acre) 1.00E+02 1.00E+03 1.00E+04 1.00E+05 1.00E+06 1.00E+07 1.00E+08 1.00E+09 15000 1.50E+06 1.50E+07 1.50E+08 1.50E+09 1.50E+10 1.50E+11 1.50E+12 1.50E+13 16000 1.60E+06 1.60E+07 1.60E+08 1.60E+09 1.60E+10 1.60E+11 1.60E+12 1.60E+13 17000 1.70E+06 1.70E+07 1.70E+08 1.70E+09 1.70E+10 1.70E+11 1.70E+12 1.70E+13 18000 1.80E+06 1.80E+07 1.80E+08 1.80E+09 1.80E+10 1.80E+11 1.80E+12 1.80E+13 19000 1.90E+06 1.90E+07 1.90E+08 1.90E+09 1.90E+10 1.90E+11 1.90E+12 1.90E+13 20000 2.00E+06 2.00E+07 2.00E+08 2.00E+09 2.00E+10 2.00E+11 2.00E+12 2.00E+13 21000 2.10E+06 2.10E+07 2.10E+08 2.10E+09 2.10E+10 2.10E+11 2.10E+12 2.10E+13 22000 2.20E+06 2.20E+07 2.20E+08 2.20E+09 2.20E+10 2.20E+11 2.20E+12 2.20E+13 23000 2.30E+06 2.30E+07 2.30E+08 2.30E+09 2.30E+10 2.30E+11 2.30E+12 2.30E+13 24000 2.40E+06 2.40E+07 2.40E+08 2.40E+09 2.40E+10 2.40E+11 2.40E+12 2.40E+13 25000 2.50E+06 2.50E+07 2.50E+08 2.50E+09 2.50E+10 2.50E+11 2.50E+12 2.50E+13 26000 2.60E+06 2.60E+07 2.60E+08 2.60E+09 2.60E+10 2.60E+11 2.60E+12 2.60E+13 27000 2.70E+06 2.70E+07 2.70E+08 2.70E+09 2.70E+10 2.70E+11 2.70E+12 2.70E+13 28000 2.80E+06 2.80E+07 2.80E+08 2.80E+09 2.80E+10 2.80E+11 2.80E+12 2.80E+13 29000 2.90E+06 2.90E+07 2.90E+08 2.90E+09 2.90E+10 2.90E+11 2.90E+12 2.90E+13 30000 3.00E+06 3.00E+07 3.00E+08 3.00E+09 3.00E+10 3.00E+11 3.00E+12 3.00E+13 31000 3.10E+06 3.10E+07 3.10E+08 3.10E+09 3.10E+10 3.10E+11 3.10E+12 3.10E+13 32000 3.20E+06 3.20E+07 3.20E+08 3.20E+09 3.20E+10 3.20E+11 3.20E+12 3.20E+13 33000 3.30E+06 3.30E+07 3.30E+08 3.30E+09 3.30E+10 3.30E+11 3.30E+12 3.30E+13 34000 3.40E+06 3.40E+07 3.40E+08 3.40E+09 3.40E+10 3.40E+11 3.40E+12 3.40E+13 35000 3.50E+06 3.50E+07 3.50E+08 3.50E+09 3.50E+10 3.50E+11 3.50E+12 3.50E+13 36000 3.60E+06 3.60E+07 3.60E+08 3.60E+09 3.60E+10 3.60E+11 3.60E+12 3.60E+13 37000 3.70E+06 3.70E+07 3.70E+08 3.70E+09 3.70E+10 3.70E+11 3.70E+12 3.70E+13 38000 3.80E+06 3.80E+07 3.80E+08 3.80E+09 3.80E+10 3.80E+11 3.80E+12 3.80E+13 39000 3.90E+06 3.90E+07 3.90E+08 3.90E+09 3.90E+10 3.90E+11 3.90E+12 3.90E+13 40000 4.00E+06 4.00E+07 4.00E+08 4.00E+09 4.00E+10 4.00E+11 4.00E+12 4.00E+13 41000 4.10E+06 4.10E+07 4.10E+08 4.10E+09 4.10E+10 4.10E+11 4.10E+12 4.10E+13

[0286] For furrow treatment embodiments, the microorganisms of the invention can be applied at a concentration of CFU per acre: 1×10 ⁶ , 3.20×10 ¹⁰ , 1.60×10 ¹¹ , 3.20×10 ¹¹ , 8.0× 10 ¹¹ , 1.6×10 ¹² , 3.20×10 ¹² or more. Thus, in certain aspects, the liquid furrow treatment compositions can be applied at a concentration of from about 1×10 ⁶ to about 3×10 ¹² CFU per acre.

[0287] In certain aspects, the furrow treatment compositions are contained in a liquid formulation. In embodiments for fluid treatment in the furrow, the microorganisms may be in a concentration of CFU per milliliter: 1×10 ¹ , 1×10 ² , 1×10 ³ , 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ , 1×10 ⁸ , 1×10 ⁹ , 1×10 ¹⁰ , 1×10 ¹¹ , 1×10 ¹² , 1×10 ¹³ or more. In certain aspects, the liquid furrow treatment compositions contain microorganisms at a concentration of from about 1×10 ⁶ to about 1×10 ¹¹ cfu per milliliter. In other aspects, the liquid furrow treatment compositions contain microorganisms at a concentration of from about 1×10 ⁷ to about 1×10 ¹⁰ CFU per milliliter. In other aspects, the liquid furrow treatment compositions contain microorganisms at a concentration of from about 1×10 ⁸ to about 1×10 ⁹ cfu per milliliter. In other aspects, the liquid furrow treatment compositions contain microorganisms at a concentration of up to about 1×10 ¹³ cfu per milliliter.

EXAMPLES

[0288] The examples provided herein describe methods for isolating bacteria, analyzing bacteria and plants, and improving plant traits. The examples are provided for illustrative purposes only and should not be construed as limiting in any way.

Example 1 Isolation of Microorganisms from Plant Tissue

[0289] Topsoil was obtained in various agricultural areas of central California. Received twenty soils with different structural characteristics, including dense clay, peat loam, silty clay and loam. Seeds of various field corn, sweet corn, old non-hybrid corn and tomato were planted in each soil as shown in Table 1 .

crop type field corn sweet corn Old non-hybrid corn Tomato Varieties Mo17 Ferry-Morse "Golden Cross Bantam T-51" Victory Seeds "Moseby Prolific" Ferry-Morse Roma VF B73 Ferry-Morse "Silver Queen Hybrid" Victory Seeds "Reid's Yellow Dent" Stover Roma DKC 66-40 Ferry-Morse "Sugar Dots" Victory Seeds "Hickory King" Totally Tomatoes "Micro Tom Hybrid" DKC 67-07 Heinz 1015 DKC 70-01 Heinz 2401 Heinz 3402 Heinz 5508 Heinz 5608 Heinz 8504

Table 1: Types and varieties of crops planted in soils with different characteristics

[0290] Plants were uprooted after 2-4 weeks of growth and excess soil on root surfaces was removed with deionized water. After removing the soil from the plants, the surface was sterilized with whitewash and intensively washed in sterile water. A 1 cm section of the cleaned root was excised from the plant and placed in phosphate-buffered saline containing 3 mm steel pellets. By vigorously stirring the solution on a Qiagen TissueLyser II, a slurry was obtained.

[0291] A suspension of root and saline was diluted and inoculated onto various types of growth media to isolate rhizospheric, endophytic, epiphytic, and other plant-associated microorganisms. R2A and Nfb agarose media were used to obtain single colonies, and Nfb semi-solid slant media were used to obtain populations of nitrogen-fixing bacteria. After 2-4 weeks of incubation in semi-solid Nfb slants, microorganism populations were collected and streaked onto R2A agar to obtain single colonies as shown in Figure 1A-B . Single colonies were resuspended in a mixture of R2A and glycerol, subjected to PCR analysis and frozen at -80°C for further analysis. Approximately 1000 single colonies were obtained and referred to as "isolated microorganisms".

[0292] The isolates were then colony screened by PCR to detect the presence of nifH genes to identify diazotrophs. To analyze the presence of the nif cluster in each isolate, the previously described Ueda 19F/388R primer set was used, which has been shown to detect more than 90% of diazotrophs in screenings (Ueda et al. 1995; J. Bacteriol. 177: 1414-1417). Single colonies of isolated isolates were punctured, resuspended in PBS and used as template for colony PCR as shown in Figure 2 . Colonies of isolates for which positive PCR bands were obtained were streaked again, and colony PCR and streaked were repeated twice to prevent false positive identification of diazotrophs. The isolated isolates were then designated as "candidate microorganisms".

Example 2 Characterization of Isolated Microorganisms

Sequencing, analysis and phylogenetic characterization

[0293] 16S rDNA sequencing with primer set 515f-806r was used to obtain preliminary phylogenetic identification characteristics of isolated microorganisms and candidate microorganisms (see, for example, Vernon et al.; BMC Microbiol. 2002 Dec 23;2:39.). Microorganisms contained various genera including: Enterobacter , Burkholderia , Klebsiella , Bradyrhizobium , Rahnella , Xanthomonas , Raoultella , Pantoea , Pseudomonas , Brevundimonas , Agrobacterium , and Paenibacillus , as shown in Table 2 .

Genus Isolates Genus Isolates Achromobacter 7 Paenibacillus 1 Agrobacterium 117 Paenisporosarcina 3 Agromyces 1 pantoea 14 Alicyclobacillus 1 Pedobacter 16 Asticcacaulis 6 Pimelobacter 2 bacillus 131 Pseudomonas 212 Bradyrhizobium 2 Rhizobium 4 Brevibacillus 2 Rhodoferax 1 Burkholderia 2 Sphingobacterium 13 Caulobacter 17 Sphingobium 23 Chryseobacterium 42 Spingomonas 3 Comamonas 1 Sphingopyxis 1 Dyadobacter 2 Stenotrophomonas 59 Flavobacterium 46 Streptococcus 3 Halomonas 3 Variovorax 37 Leptothrix 3 Xylanimicrobium 1 Lysobacter 2 unidentified 75 Neisseria 13

Table 2: Diversity of microorganisms isolated from tomato plants as determined by 16S rDNA deep sequencing.

[0294] The genomes of 39 candidate microorganisms were then sequenced using the Illumina Miseq platform. Genomic DNA from pure cultures was isolated using a QIAmp DNA mini kit (QIAGEN) and total DNA libraries for sequencing were obtained from an independent supplier (SeqMatic, Hayward). The genome was then assembled using the A5 pipeline (Tritt et al., 2012; PLoS One 7(9):e42304). Genes were identified and annotated, and genes associated with the regulation and expression of nitrogen fixation were marked as targets of mutagenesis.

Transcriptome profiling of candidate microorganisms

[0295] To identify promoters that are active in the presence of environmental nitrogen, transcriptomic profiling of strain CI010 was performed. The CI010 strain was cultured in a defined nitrogen-free medium supplemented with 10 mM glutamine. Total RNA was isolated from these cultures (QIAGEN RNeasy kit) and subjected to RNAseq sequencing by HiSeq Illumina (SeqMatic, Fremont CA). Sequencing reads were mapped to CI010 genome data using Geneious and identified highly expressed genes under the control of proximal transcription promoters.

[0296] Tables 3A-C list genes and their relative expression levels as determined by total RNA RNAseq sequencing. Proximal promoter sequences were registered for use in mutagenesis of nif metabolic pathways, nitrogen utilization metabolic pathways, or other genes at the desired level of expression.

Table 3A

Name Minimum Maximum Length Direction murein lipoprotein CDS 2929898 2930134 237 direct membrane protein CDS 5217517 5217843 327 direct CDS of zinc/cadmium binding protein 3479979 3480626 648 direct CDS of acyl carrier protein 4563344 4563580 237 reverse CDS ompX 4251002 4251514 513 direct CDS of DNA-binding protein HU-beta 375.156 375.428 273 direct CDS sspA 629.998 630.636 639 reverse CDS tatE 3199435 3199638 204 reverse CDS of the LexA repressor 1850457 1851065 609 direct CDS hisS <3999979 4001223 >1245 direct

Table 3B

Name absolute reliability of differential expression Differential expression ratio RNAseq_nifL - Total number of reads RNAseq_nifL - Total number of transcripts RNAseq_WT - Total number of reads RNAseq_WT - Total number of transcripts murein lipoprotein CDS 1000 -1.8 12950.5 10078.9 5151.5 4106.8 membrane protein CDS 1000 -1.3 9522.5 5371.3 5400 3120 CDS of zinc/cadmium binding protein 3.3 1.1 6461 1839.1 5318 1550.6 CDS of acyl carrier protein 25.6 1.6 1230.5 957.6 1473.5 1174.7 CDS ompX 1.7 1.1 2042 734.2 1687.5 621.5 CDS of DNA-binding protein HU-beta 6.9 -1.3 1305 881.7 725 501.8 CDS sspA 0.2 1 654 188.8 504.5 149.2 CDS tatE 1.4 1.3 131 118.4 125 115.8 CDS of the LexA repressor 0.1 -1.1 248 75.1 164 50.9 CDS hisS 0 -1.1 467 69.2 325 49.3

Table 3C

Strain table

Variety First indication Current name Universal name Line Description of mutant DNA Genotype Gene mutation 1 Gene mutation 2 1 Application text CI006 CI006 A strain isolated from the genera Enterobacter No wt 2 Application text CI008 CI008 A strain isolated from the genera Burkholderia No wt 3 Application text CI010 CI010 A strain isolated from the genera Klebsiella No wt 4 Application text CI019 CI019 A strain isolated from the genera Rahnella No wt 5 Application text CI028 CI028 A strain isolated from the genera Enterobacter No wt 6 Application text CI050 CI050 A strain isolated from the genera Klebsiella No wt 7 Application text CM002 CM002 Mutant CI050 Destruction of the nifL gene by the kanamycin resistance (KanR) expression cassette encoding the built-in aph1 aminoglycoside-O-phosphotransferase gene. ΔnifL::KanR SEQ ID NO:33 8 Application text CM011 CM011 Mutant CI019 Destruction of the nifL gene by a spectinomycin resistance expression cassette (SpecR) encoding an inserted streptomycin-3''-O-adenylate transferase aadA gene. ΔnifL::SpecR SEQ ID NO:34 9 Application text CM013 CM013 Mutant CI006 Destruction of the nifL gene by the kanamycin resistance (KanR) expression cassette encoding the built-in aph1 aminoglycoside-O-phosphotransferase gene. ΔnifL::KanR SEQ ID NO:35 10 figure 4A CM004 CM004 Mutant CI010 Destruction of the amtB gene by the kanamycin resistance (KanR) expression cassette encoding the built-in aph1 aminoglycoside-O-phosphotransferase gene. ΔamtB::KanR SEQ ID NO:36 eleven figure 4A CM005 CM005 Mutant CI010 Destruction of the nifL gene by the kanamycin resistance (KanR) expression cassette encoding the built-in aph1 aminoglycoside-O-phosphotransferase gene. ΔnifL::KanR SEQ ID NO:37 12 figure 4B CM015 CM015 Mutant CI006 Destruction of the nifL gene by the built-in fragment of the region upstream of the ompX gene (Prm5). ΔnifL::Prm5 SEQ ID NO:38 13 figure 4B CM021 CM021 Mutant CI006 Destruction of the nifL gene by an inserted fragment of the region upstream of the unannotated gene and the first 73 b.p. this gene (Prm2). ΔnifL::Prm2 SEQ ID NO:39 14 figure 4B CM023 CM023 Mutant CI006 Destruction of the nifL gene by the built-in fragment of the region upstream of the acpP gene and the first 121 b.p. acpP gene (Prm4). ΔnifL::Prm4 SEQ ID NO:40 15 figure 10A CM014 CM014 Mutant CI006 Destruction of the nifL gene by the built-in fragment of the region upstream of the lpp gene and the first 29 b.p. lpp gene (Prm1). ΔnifL::Prm1 SEQ ID NO:41 16 figure 10A CM016 CM016 Mutant CI006 Destruction of the nifL gene by the built-in fragment of the region upstream of the lexA gene 3 and the first 21 b.p. lexA 3 gene (Prm9). ΔnifL::Prm9 SEQ ID NO:42 17 figure 10A CM022 CM022 Mutant CI006 Destruction of the nifL gene by the built-in fragment of the region upstream of the mntP 1 gene and the first 53 b.p. mntP 1 gene (Prm3). ΔnifL::Prm3 SEQ ID NO:43 18 figure 10A CM024 CM024 Mutant CI006 Destruction of the nifL gene by the built-in fragment of the region upstream of the sspA gene (Prm7). ΔnifL::Prm7 SEQ ID NO:44 19 figure 10A CM025 CM025 Mutant CI006 Destruction of the nifL gene by an inserted fragment of the region upstream of the hisS gene and the first 52 bp. hisS gene (Prm10). ΔnifL::Prm10 SEQ ID NO:45 20 figure 10B CM006 CM006 Mutant CI010 Destruction of the glnB gene by the kanamycin resistance (KanR) expression cassette encoding the built-in aph1 aminoglycoside-O-phosphotransferase gene. ΔglnB::KanR SEQ ID NO:46 21 figure 10C CI028 nifL:KanR CM017 Mutant CI028 Destruction of the nifL gene by the kanamycin resistance (KanR) expression cassette encoding the built-in aph1 aminoglycoside-O-phosphotransferase gene. ΔnifL::KanR SEQ ID NO:47 22 figure 10C CI019 nifL:SpecR CM011 Mutant CI019 Destruction of the nifL gene by a spectinomycin resistance expression cassette (SpecR) encoding an inserted streptomycin-3''-O-adenylate transferase aadA gene. ΔnifL::SpecR SEQ ID NO:48 23 figure 10C CI006 nifL:KanR CM013 Mutant CI006 Destruction of the nifL gene by the kanamycin resistance (KanR) expression cassette encoding the built-in aph1 aminoglycoside-O-phosphotransferase gene. ΔnifL::KanR SEQ ID NO:49 24 figure 10C CI010 nifL:KanR CM005 Mutant CI010 Destruction of the nifL gene by the kanamycin resistance (KanR) expression cassette encoding the built-in aph1 aminoglycoside-O-phosphotransferase gene. ΔnifL::KanR SEQ ID NO:50 25 figure 4C Strain 2 CI006 A strain isolated from the genera Enterobacter No wt 26 figure 4C Strain 4 CI010 A strain isolated from the genera Klebsiella No wt 27 figure 4C Strain 1 CI019 A strain isolated from the genera Rahnella No wt 28 figure 4C Strain 3 CI028 A strain isolated from the genera Enterobacter No wt 29 figure 4B Strain 2 CI006 A strain isolated from the genera Enterobacter No wt thirty figure 4B high CM014 Mutant CI006 Destruction of the nifL gene by the built-in fragment of the region upstream of the lpp gene and the first 29 b.p. lpp gene (Prm1) ΔnifL::Prm1 SEQ ID NO:51 31 figure 4B Med CM015 Mutant CI006 Destruction of the nifL gene by an integrated fragment of the region upstream of the ompX gene (Prm5). ΔnifL::Prm5 SEQ ID NO:52 32 figure 4B low CM023 Mutant CI006 Destruction of the nifL gene by the built-in fragment of the region upstream of the acpP gene and the first 121 b.p. acpP gene (Prm4). ΔnifL::Prm4 SEQ ID NO:53 33 figure 4D Strain 2 CI006 A strain isolated from the genera Enterobacter No wt 34 figure 4D evolved CM029 Mutant CI006 Destruction of the nifL gene by an integrated fragment of the region upstream of the ompX gene (Prm5) and a 1287 bp deletion. after the start codon of the glnE gene containing the adenylyl-deleting domain of the adenylate transferase glutamate ammonia ligase (ΔglnE-AR_KO1). ΔnifL::Prm5 ΔglnE-AR_KO1 SEQ ID NO:54 SEQ ID NO:61 35 figure 14C wild type CI006 A strain isolated from the genera Enterobacter No wt 36 figure 14C evolved CM014 Mutant CI006 Destruction of the nifL gene by the built-in fragment of the region upstream of the lpp gene and the first 29 b.p. lpp gene (Prm1) ΔnifL::Prm1 SEQ ID NO:55 37 figure 14B wild type CI019 A strain isolated from the genera Rahnella No wt 38 figure 14B evolved CM011 Mutant CI019 Destruction of the nifL gene by a spectinomycin resistance expression cassette (SpecR) encoding an inserted streptomycin-3''-O-adenylate transferase aadA gene. ΔnifL::SpecR SEQ ID NO:56 39 figure 14A evolved CM011 Mutant CI019 Destruction of the nifL gene by a spectinomycin resistance expression cassette (SpecR) encoding an inserted streptomycin-3''-O-adenylate transferase aadA gene. ΔnifL::SpecR SEQ ID NO:57 40 figure 15A wild type CI006 A strain isolated from the genera Enterobacter No wt 41 figure 15A evolved CM013 Mutant CI006 Destruction of the nifL gene by the kanamycin resistance (KanR) expression cassette encoding the built-in aph1 aminoglycoside-O-phosphotransferase gene. ΔnifL::KanR SEQ ID NO:58 42 figure 15B Untitled CM011 Mutant CI019 Destruction of the nifL gene by a spectinomycin resistance expression cassette (SpecR) encoding an inserted streptomycin-3''-O-adenylate transferase aadA gene. ΔnifL::SpecR SEQ ID NO:59 43 figure 16B Strain 5 CI008 A strain isolated from the genera Burkholderia No wt 44 figure 16B Strain 1 CM011 Mutant CI019 Destruction of the nifL gene by a spectinomycin resistance expression cassette (SpecR) encoding an inserted streptomycin-3''-O-adenylate transferase aadA gene. ΔnifL::SpecR SEQ ID NO:60

Strain Sequence Table

SEQID NO: Subsequence 33 ATGAGCCATATTCAACGGGAAACGTCTTGCTCCAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGA CGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAAT GAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTTCTCACTTGATAACCTTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACT GCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGAATGCTCGATGAGTTTTTCTAATAAGCCTGCCTGGTTCTGCGTTTCCCGCTCTTTAATACCCTGACCGGAGGTGAGCAATGA 34 ATGAGCATCACGGCGTTATCAGCATCATTTCCTGAGGGGAATATCGCCAGCCGCTTGTCGCTGCAACATCCTTCACTGTTTTATACCGTGGTTGAACAATCTTCGGTGGCGAGCGTGTTGAGTCATCCTGACTAGCTGAGATGAGGGCTCGCCCCCTCGTCCCGACACTTCCAGATCGCCATAGCGCACAGCGCCTCGAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGC TTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCCATCTCGAACCGACG TTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGC AATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCGACTGGGCTGGCGATGAGCGAAATGTA GTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAAT GTCTAACAATTCGTTCAAGCCGACGCCGCTTCGGCGCGGCTTAACTCAAGCGTTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAGGTCAAGTCTGCTTTTTATTTTTTAAGCGTGCATAATAAGCCCTACACAAATGGTACCCGACCGGTGGTGAATTTAATCTCGCTGACGTGTAGACATTCCCTTATCCAGACGCTGATCGCCCATCATCGCGGTTCTTTAGATCT CTCGGTCCGCCCTGATGGCGGCACCTTGCTGACGTTACGCCTGCCGGTACAGCAGGTTATCACCGGAGGCTTAAAATGA 35 CTGATCCTTCAACTCAAGCAAAAGTTCGATTTATTCAACAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCCAGGCCGGATTAAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGC GATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCAGG TATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCAT TCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTC GATGAGTTTTTCTAATAAGCCTTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGA 36 ATGAAGATAGCAACAATGAAAACAGGTCTGGGAGCGTTGGCTCTTCTTCCCTGATCCTTCAACTCAAGCAAAAGTTCGATTTATTCAACAAAGCCACGTTGTGTCTCAAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCCCGTCCG CGCTTAAACTCCAACATGGACGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCCGTCTCAACTGGCTGTCTCAACTGCTGACGGAGTTTATGCCTCTCCCGACCATCAAGCATTTTATCCGT ACTCCTGATGATGCGTGGTTACTCACCACCGCGATTCCTGGGAAAACAGCCTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCCGTGTTCCTGCGCCGGTTACATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGTGTATTTCGTCTTGCTCAGGGCCAATCACGCATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGA CGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCACAAGCTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTTCTCACTTGATAACCTTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGGGTCGGAATCGCAGACCGTTACCAGACCTTGCCATTCTTTGGAACTGCCTCGGTGAGTTTTCCTTCATTACAGAAA CGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATAAGCCTGTGAAGGGCTGGACGTAAACAGCCACGGCGAAAACGCCTACAACGCCTGA 37 ATGACCCTGAATATGATGCTCGATAACGCCGTACCCGAGGCGATTGCCGGCTGATCCTTCAACTCAAGCAAAAGTTCGATTTATTCAACAAAGCCACGTTGTGTCTCAAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCCCGTCCGCGCT TAAACTCCAACATGGACGCTGATTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCCGTCTCAACTGGCTGACGGAGTTTATGCCTCTCCCGACCATCAAGCATTTTATCCGTACTCC TGATGATGCGTGGTTACTCACCACCGCGATTCCTGGGAAAACAGCCTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCCGTGTTCCTGCGCCGGTTACATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGTGTATTTCGTCTTGCTCAGGCGCAATCACGCATGAATAACGTTTGGTTGATGCGAGTGATTTTGATGACGAG CGTAATGGCTGGCCTGTTGAACTCTGGAAAGAAATGCACAAGCTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGGGTCGGAATCGCAGACCGTTACCAGGACCTTGCCATTCTTTGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGC TTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGAATGCTCGATGAGTTTTTCTAATAAGCCTTGGTTCTGCGTTTCCCGCTCTTTAATACCCTGACCGGAGGTGAGCAATGA 38 ATGACCCTGAATATGATGATGGATGCCGGCGGACATCATCGCGACAAACAATATTAATACCGGCAACCACCGGCAATTTACGAGACTGCGCAGGCATCCTTTCTCCGTCAATTTCTGTCAAATAAAGTAAAAGAGGCAGTCTACTTGAATTACCCCCGGCTGGTTGAGCGTTTGTTGAAAAAAAAGTAACTGAAAAATCCGTAGAATAGCGCCACTCTGATGGTTAATTAACCTAT TCAATTAAGAATTATCTGGATGAATGTGCCATTAAATGCGCAGCATAATGGTGCGTTGTGCGGGAAAACTGCTTTTTTTTGAAAGGGTTGGTCAGTAGCGGAAACAACTCACTTCACACCCCGAAGGGGGAAGTTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGA 39 ATGACCCTGAATATGATGATGGATGCCGGCTCACCACGGCGATAACCATAGGTTTTCGGCGTGGCCACATCCATGGTGAATCCCACTTTTTCCAGCACGCGCGCCACTTCATCGGGTCTTAAATACATAGATTTTCCTCGTCATCTTTCCAAAGCCTCGCCACCTTACATGACTGAGCATGGACCGTGACTCAGAAAATTCCACAAACGAACCTGAAAGGCGTGATTGCCGTCTGGCCT TAAAAATTATGGTCTAAACTAAAATTTACATCGAAAACGAGGGAGGATCCTATGTTTAACAAACCGAATCGCCGTGACGTAGATGAAGGTGTTGAGGATATTAACCACGATGTTAACCAGCTCGAACTCACTTCACACCCCGAAGGGGGAAGTTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGA 40 ATGACCCTGAATATGATGATGGATGCCGGCTGACGAGGCAGGTTACATCACTGGTGAAACCCTGCACGTCAATGGCGGAATGTATATGGTTTAACCACGATGAAAATTATTTGCGTTATTAGGGCGAAAGGCCTCAAAATAGCGTAAAATCGTGGTAAGAACTGCCGGGATTAGTTGCAAATTTTCAACATTTTATACACTACGAAAACCATCGCGAAAGCGAGTTTT GATAGGAAATTTAAGAGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCCTTCGTTGAAGACCTGGGCGCTGATTCTCTTGACACCGAACTCACTTCACACCCCGAAGGGGGAAGTTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGA 41 ATGACCCTGAATATGATGATGGATGCCGGCCGTCCTGTAATAATAACCGGACAATTCGGACTGATTAAAAAAGCGCCCTTGTGGCGCCTTTTTTTATATTCCCGCCTCCATTTAAAATAAAAAATCCAATCGGATTTCACTATTTAAACTGGCCATTATCTAAGATGAATCCGATGGAAGCTCGCTGTTTTAACACGCGTTTTAACCTTTTATTGAAAGTCGGTGCTTCTTTGAGCGAA CGATCAAATTTAAGTGGATTCCCATCAAAAAAAATATTCTCAACCTAAAAAAGTTTGTGTAATACTTGTAACGCTACATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAATCGTACTAAACTGGTACTGGGCGCAACTCACTTCACACCCCGAAGGGGGAAGTTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGA 42 ATGACCCTGAATATGATGATGGATGCCGGCATATTGACACCATGACGCGCGTAATGCTGATTGGTTCTGTGACGCTGGTAATGATTGTCGAAATTCTGAACAGTGCCATCGAAGCCGTAGTAGACCGTATTGGTGCAGAATTCCATGAACTTTCCGGGCGGGCGAAGGATATGGGGTCGGCGGCGGTGCTGATGTCCATCCTGCTGGCGATGTTTACCTGGATCG CATTACTCTGGTCACATTTTCGATAACGCTTCCAGAATTCGATAACGCCCTGGTTTTTTGCTTAAATTTGGTTCCAAAATCGCCTTTAGCTGTATATACTCACAGCATAACTGTATATACACCCAGGGGGCGGGATGAAAGCATTAACGGCCAGGAACTCACTTCACACCCCGAAGGGGAAGTTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCA AAATGA 43 ATGACCCTGAATATGATGATGGATGCCGGCATCATATTGCGCTCCCTGGTTATCATTTGTTACTAAATGAAATGTTATAATATAACAATTATAAATACCACATCGCTTTCAATTCACCAGCCAAATGAGGAGCGCCGTCTGACATAGCCAGCGCTATAAAACATAGCATTATCTATATGTTTATGATTAATAACTGATTTTTGCGTTTTGGATTTGGCTGTGGCATCCTTGCCGCTCTTTTCGCAG CGTCTGCGTTTTTGCCCTCCGGTCAGGGCATTTAAGGGTCAGCAATGAGTTTTTACGCAATTACGATTCTTGCCTTCGGCATGTCGATGGATTGCTTTAACTCACTTCACACCCCCGAAGGGGGAAGTTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGA 44 ATGACCCTGAATATGATGATGGATGCCGGCCGCGTCAGGTTGAACGTAAAAAAGTCGGTCTGCGCAAAGCACGTCGTCGTCCGCAGTTCTCCAAACGTTAATTGGTTTCTGCTTCGGCAGAACGATTGGCGAAAAAACCCGGTGCGAACCGGGTTTTTTTATGGATAAAGATCGTGTTATCCACAGCAATCCATTGATTATCTCTTCTTTTTCAGCATTTCCAG AATCCCCTCACCACAAAGCCCGCAAAATCTGGTAAACTATCATCCAATTTTCTGCCCAAATGGCTGGGATTGTTCATTTTTTGTTTGCCTTACAACGAGAGTGACAGTACGCGCGGGTAGTTAACTCAACATCTGACCGGTCGATAACTCACTTCACACCCCGAAGGGGGAAGTTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGA 45 ATGACCCTGAATATGATGATGGATGCCGGCCCTGTATGAAGATGGCGTGCGCAAAGATCGCCTGGATAACAGCGATATGATTAGCCAGCTTGAAGCCCGCATTCGCGCGAAAAGCGTCAATGCTGGACGAAGCGCGTCGTATCGATGTGCAACAGGTAGAAAAATAAGGTTGCTGGGAAGCGGCAGGCTTCCCGTGTATGATGAACCCGCCCGGCGCGACCCGTTGTTCGTCGC GGCCCCGAGGGTTCATTTTTTGTATTAATAAAGAGAATAAACGTGGCAAAAAATATTCAAGCCATTCGGCATGAACGATTATCTGCCTGGCGAACTCACTTCACACCCCGAAGGGGAAGTTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGA 46 ATGAAAAAGATTGATGCGATTATTAAACCTTTCAAACTGGATGACGTGCGCTGATCCTTCAACTCAAGCAAAAGTTCGATTTATTCAACAAAGCCACGTTGTGTCTCAAAAATCTCTGATGTTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCCCCGTCCGCG CTTAAACTCCAACATGGACGCTGATTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCCGTCTCAACTGGCTGGACGGAGTTTATGCCTCTCCCGACCATCAAGCATTTTATCCGTACT CCTGATGATGCGTGGTTACTCACCACCGCGATTCCTGGGAAAACAGCCTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCCGTGTTCCTGCGCCGGTTACATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGTGTATTTCGTCTTGCTCAGGCGCAATCACGCATGAATAACGTTTGGTTGATGCGAGTGATTTTGATGACG AGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCACAAGCTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTTCTCACTTGATAACCTTTTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGGGTCGGAATCGCAGACCGTTACCAGGACCTTGCCATTCTTTGGAACTGCCTCGGTGAGTTTTCCTTCATTACAGAAACG GCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATAAGCCTCGCGCGTGATTCGTATCCGCACCGGCGAAGAAGACGACGCGGCGATTTAA 47 ATGACCATGAACCTGATGACGGATGTCGTCTCAGCCACCGGGATCGCCGGGTTGCTTTCACGACAACCCGACGCTGTTTTTTACACTAATTGAACAGGCCCCCGTGGCGATCACCGCTGACGGATACCGCTGCCCGCATTGTCTATGCCAACCCGGGCGTGTTGAGTCATCCTGACTAGCTGAGATGAGGGCTCGCCTGATTCCTTCAACTCAGCAAAAGTTCGATTTATTCAA CAAAGCCACGTTGTGTCTCAAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCCAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCG ATTGTATGGGAAGCCCGATGCCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTT GATGGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTC ACTTGATAACCTTATTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATAAGCCTGACCGGTGGTGAATTTA ATCTCGCTGACGTGTAGACATTCATCGATCTGCATCCACGGTCCGGCGGCGGTACCTGCCTGACGCTACGTTTACCGCTCTTTTATGAACTGACCGGAGGCCCAAGATGA 48 ATGAGCATCACGGCGTTATCAGCATCATTTCCTGAGGGGAATATCGCCAGCCGCTTGTCGCTGCAACATCCTTCACTGTTTTATACCGTGGTTGAACAATCTTCGGTGGCGAGCGTGTTGAGTCATCCTGACTAGCTGAGATGAGGGCTCGCCCCCTCGTCCCGACACTTCCAGATCGCCATAGCGCACAGCGCCTCGAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGC TTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCCATCTCGAACCGACG TTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGC AATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCGACTGGGCTGGCGATGAGCGAAATGTA GTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAAT GTCTAACAATTCGTTCAAGCCGACGCCGCTTCGGCGCGGCTTAACTCAAGCGTTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAGGTCAAGTCTGCTTTTTATTTTTTAAGCGTGCATAATAAGCCCTACACAAATGGTACCCGACCGGTGGTGAATTTAATCTCGCTGACGTGTAGACATTCCCTTATCCAGACGCTGATCGCCCATCATCGCGGTTCTTTAGATCT CTCGGTCCGCCCTGATGGCGGCACCTTGCTGACGTTACGCCTGCCGGTACAGCAGGTTATCACCGGAGGCTTAAAATGA 49 CTGATCCTTCAACTCAAGCAAAAGTTCGATTTATTCAACAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCCAGGCCGGATTAAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGC GATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCAGG TATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCAT TCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTC GATGAGTTTTTCTAATAAGCCTTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGA 50 ATGACCCTGAATATGATGCTCGATAACGCCGTACCCGAGGCGATTGCCGGCTGATCCTTCAACTCAAGCAAAAGTTCGATTTATTCAACAAAGCCACGTTGTGTCTCAAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCCCGTCCGCGCT TAAACTCCAACATGGACGCTGATTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCCGTCTCAACTGGCTGACGGAGTTTATGCCTCTCCCGACCATCAAGCATTTTATCCGTACTCC TGATGATGCGTGGTTACTCACCACCGCGATTCCTGGGAAAACAGCCTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCCGTGTTCCTGCGCCGGTTACATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGTGTATTTCGTCTTGCTCAGGCGCAATCACGCATGAATAACGTTTGGTTGATGCGAGTGATTTTGATGACGAG CGTAATGGCTGGCCTGTTGAACTCTGGAAAGAAATGCACAAGCTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGGGTCGGAATCGCAGACCGTTACCAGGACCTTGCCATTCTTTGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGC TTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGAATGCTCGATGAGTTTTTCTAATAAGCCTTGGTTCTGCGTTTCCCGCTCTTTAATACCCTGACCGGAGGTGAGCAATGA 51 ATGACCCTGAATATGATGATGGATGCCGGCCGTCCTGTAATAATAACCGGACAATTCGGACTGATTAAAAAAGCGCCCTTGTGGCGCCTTTTTTTATATTCCCGCCTCCATTTAAAATAAAAAATCCAATCGGATTTCACTATTTAAACTGGCCATTATCTAAGATGAATCCGATGGAAGCTCGCTGTTTTAACACGCGTTTTAACCTTTTATTGAAAGTCGGTGCTTCTTTGAGCGAA CGATCAAATTTAAGTGGATTCCCATCAAAAAAAATATTCTCAACCTAAAAAAGTTTGTGTAATACTTGTAACGCTACATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAATCGTACTAAACTGGTACTGGGCGCAACTCACTTCACACCCCGAAGGGGGAAGTTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGA 52 ATGACCCTGAATATGATGATGGATGCCGGCGGACATCATCGCGACAAACAATATTAATACCGGCAACCACCGGCAATTTACGAGACTGCGCAGGCATCCTTTCTCCGTCAATTTCTGTCAAATAAAGTAAAAGAGGCAGTCTACTTGAATTACCCCCGGCTGGTTGAGCGTTTGTTGAAAAAAAAGTAACTGAAAAATCCGTAGAATAGCGCCACTCTGATGGTTAATTAACCTAT TCAATTAAGAATTATCTGGATGAATGTGCCATTAAATGCGCAGCATAATGGTGCGTTGTGCGGGAAAACTGCTTTTTTTTGAAAGGGTTGGTCAGTAGCGGAAACAACTCACTTCACACCCCGAAGGGGGAAGTTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGA 53 ATGACCCTGAATATGATGATGGATGCCGGCTGACGAGGCAGGTTACATCACTGGTGAAACCCTGCACGTCAATGGCGGAATGTATATGGTTTAACCACGATGAAAATTATTTGCGTTATTAGGGCGAAAGGCCTCAAAATAGCGTAAAATCGTGGTAAGAACTGCCGGGATTAGTTGCAAATTTTCAACATTTTATACACTACGAAAACCATCGCGAAAGCGAGTTTT GATAGGAAATTTAAGAGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCCTTCGTTGAAGACCTGGGCGCTGATTCTCTTGACACCGAACTCACTTCACACCCCGAAGGGGGAAGTTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGA 54 ATGACCCTGAATATGATGATGGATGCCGGCGGACATCATCGCGACAAACAATATTAATACCGGCAACCACCGGCAATTTACGAGACTGCGCAGGCATCCTTTCTCCGTCAATTTCTGTCAAATAAAGTAAAAGAGGCAGTCTACTTGAATTACCCCCGGCTGGTTGAGCGTTTGTTGAAAAAAAAGTAACTGAAAAATCCGTAGAATAGCGCCACTCTGATGGTTAATTAACCTAT TCAATTAAGAATTATCTGGATGAATGTGCCATTAAATGCGCAGCATAATGGTGCGTTGTGCGGGAAAACTGCTTTTTTTTGAAAGGGTTGGTCAGTAGCGGAAACAACTCACTTCACACCCCGAAGGGGGAAGTTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGA 55 ATGACCCTGAATATGATGATGGATGCCGGCCGTCCTGTAATAATAACCGGACAATTCGGACTGATTAAAAAAGCGCCCTTGTGGCGCCTTTTTTTATATTCCCGCCTCCATTTAAAATAAAAAATCCAATCGGATTTCACTATTTAAACTGGCCATTATCTAAGATGAATCCGATGGAAGCTCGCTGTTTTAACACGCGTTTTAACCTTTTATTGAAAGTCGGTGCTTCTTTGAGCGAA CGATCAAATTTAAGTGGATTCCCATCAAAAAAAATATTCTCAACCTAAAAAAGTTTGTGTAATACTTGTAACGCTACATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAATCGTACTAAACTGGTACTGGGCGCAACTCACTTCACACCCCGAAGGGGGAAGTTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGA 56 ATGAGCATCACGGCGTTATCAGCATCATTTCCTGAGGGGAATATCGCCAGCCGCTTGTCGCTGCAACATCCTTCACTGTTTTATACCGTGGTTGAACAATCTTCGGTGGCGAGCGTGTTGAGTCATCCTGACTAGCTGAGATGAGGGCTCGCCCCCTCGTCCCGACACTTCCAGATCGCCATAGCGCACAGCGCCTCGAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGC TTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCCATCTCGAACCGACG TTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGC AATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCGACTGGGCTGGCGATGAGCGAAATGTA GTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAAT GTCTAACAATTCGTTCAAGCCGACGCCGCTTCGGCGCGGCTTAACTCAAGCGTTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAGGTCAAGTCTGCTTTTTATTTTTTAAGCGTGCATAATAAGCCCTACACAAATGGTACCCGACCGGTGGTGAATTTAATCTCGCTGACGTGTAGACATTCCCTTATCCAGACGCTGATCGCCCATCATCGCGGTTCTTTAGATCT CTCGGTCCGCCCTGATGGCGGCACCTTGCTGACGTTACGCCTGCCGGTACAGCAGGTTATCACCGGAGGCTTAAAATGA 57 ATGAGCATCACGGCGTTATCAGCATCATTTCCTGAGGGGAATATCGCCAGCCGCTTGTCGCTGCAACATCCTTCACTGTTTTATACCGTGGTTGAACAATCTTCGGTGGCGAGCGTGTTGAGTCATCCTGACTAGCTGAGATGAGGGCTCGCCCCCTCGTCCCGACACTTCCAGATCGCCATAGCGCACAGCGCCTCGAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGC TTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCCATCTCGAACCGACG TTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGC AATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCGACTGGGCTGGCGATGAGCGAAATGTA GTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAAT GTCTAACAATTCGTTCAAGCCGACGCCGCTTCGGCGCGGCTTAACTCAAGCGTTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAGGTCAAGTCTGCTTTTTATTTTTTAAGCGTGCATAATAAGCCCTACACAAATGGTACCCGACCGGTGGTGAATTTAATCTCGCTGACGTGTAGACATTCCCTTATCCAGACGCTGATCGCCCATCATCGCGGTTCTTTAGATCT CTCGGTCCGCCCTGATGGCGGCACCTTGCTGACGTTACGCCTGCCGGTACAGCAGGTTATCACCGGAGGCTTAAAATGA 58 CTGATCCTTCAACTCAAGCAAAAGTTCGATTTATTCAACAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCCAGGCCGGATTAAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGC GATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCAGG TATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCAT TCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTC GATGAGTTTTTCTAATAAGCCTTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGA 59 ATGAGCATCACGGCGTTATCAGCATCATTTCCTGAGGGGAATATCGCCAGCCGCTTGTCGCTGCAACATCCTTCACTGTTTTATACCGTGGTTGAACAATCTTCGGTGGCGAGCGTGTTGAGTCATCCTGACTAGCTGAGATGAGGGCTCGCCCCCTCGTCCCGACACTTCCAGATCGCCATAGCGCACAGCGCCTCGAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGC TTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCCATCTCGAACCGACG TTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGC AATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCGACTGGGCTGGCGATGAGCGAAATGTA GTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAAT GTCTAACAATTCGTTCAAGCCGACGCCGCTTCGGCGCGGCTTAACTCAAGCGTTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAGGTCAAGTCTGCTTTTTATTTTTTAAGCGTGCATAATAAGCCCTACACAAATGGTACCCGACCGGTGGTGAATTTAATCTCGCTGACGTGTAGACATTCCCTTATCCAGACGCTGATCGCCCATCATCGCGGTTCTTTAGATCT CTCGGTCCGCCCTGATGGCGGCACCTTGCTGACGTTACGCCTGCCGGTACAGCAGGTTATCACCGGAGGCTTAAAATGA 60 ATGAGCATCACGGCGTTATCAGCATCATTTCCTGAGGGGAATATCGCCAGCCGCTTGTCGCTGCAACATCCTTCACTGTTTTATACCGTGGTTGAACAATCTTCGGTGGCGAGCGTGTTGAGTCATCCTGACTAGCTGAGATGAGGGCTCGCCCCCTCGTCCCGACACTTCCAGATCGCCATAGCGCACAGCGCCTCGAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGC TTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCCATCTCGAACCGACG TTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGC AATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCGACTGGGCTGGCGATGAGCGAAATGTA GTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAAT GTCTAACAATTCGTTCAAGCCGACGCCGCTTCGGCGCGGCTTAACTCAAGCGTTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAGGTCAAGTCTGCTTTTTATTTTTTAAGCGTGCATAATAAGCCCTACACAAATGGTACCCGACCGGTGGTGAATTTAATCTCGCTGACGTGTAGACATTCCCTTATCCAGACGCTGATCGCCCATCATCGCGGTTCTTTAGATCT CTCGGTCCGCCCTGATGGCGGCACCTTGCTGACGTTACGCCTGCCGGTACAGCAGGTTATCACCGGAGGCTTAAAATGA 61 ATGTTTAACGATCTGATTGGCGATGATGAAACGGATTCGCCGGAAGATGCGCTTTCTGAGAGCTGGCGCGAATTGTGTGGCAGGATGCGTTGCAGGAGGAGGATTCCACGCCCGTGCTGGCGCATCTCTCAGGACGATCGCCGCCGCGTGGTGGCGCCTGATTGCCGATTTTCGCAAAGAGTTGGATAAACGCACCATTGGCCCGCGAGGGCGGCAGGTACTCGATCACT TAATGCCGCATCTGCTCAGCGATGTATGCTCGCGACGATGCGCCAGTACCGCTGTCACGCCTGACGCCGCTGCTCACCGGAATTATTACCCGCACCACTTACCTTGAGCCTGCTAAGTGAATTTCCCGGCGCACTGAACACCTCATTTCCCTGTGTGCCGCGTCGCCCGATGGTTGCCAGTCAGCTGGCGCTACCCGATCCTGCTTGATGAATTGCTCGACCCGAATACGCTCTAT CAACCGACGGCGATGAATGCCTATCGCGATGAGCTGCGCCAATACCTGCTGCGCGTGCCGGAAGATGAAGAGCAACAGCTTGAGGCTGCGGCAGTTTAAGCAGGCGCAGTTGCTGCGCGTGGCGGCGGCGGATATTGCCGGTACGTTGCCAGTAATGAAAGTGAGCGATCACTTAACCTGGCTGGCGGAAGCGATTATTGATGCGGTGGTGGTCAGCAAGCCTGGG GGCAGATGGTGGCGCGTTATGGCCAGCCAACGCATCTGCACGATCGCGAAGGGCGCGGTTTTGCGGTGGTCGGTTATGGCAAGCTGGGCGGCTGGGAGCTGGGTTACAGCTCCGATCTGGATCTGGTATTCCTGCACGACTGCCCGATGGATGTGATGACCGATGGCGAGCGTGAAATCGATGGTCGCCAGTTCTATTTGCGTCTCGCGCAGCGTGTGATGC ACCTGTTTAGCACGCGCACGTCGTCCGGCATCCTTTTATGAAGTTGATGCGCGTCTGCGTCCATCTGGCTGCGGGGATGCTGGTCACTACTACGGAATCGTTCGCCGATTACCACAAAACGAAGCCTGGACGTGGGAACATCAGGCGCTGGCCCGTGCGCGCGTGGTGTACGGCGATCCGCAACTGACCGCGAATTTTGACGCCATTCGCCGCGATATTCTGATGACGCCTCGC GACGGCGCAACGCTGCAAACCGACGTGCGGAAAATGCGCGAGAAAATGCGTGCCCATCTTGGCAACAAGCATAAAGACCGCTTCGATCTGAAAGCCGATGAAGGCGGTATCACCGACATCGAGTTTATCGCCCAATATCTGGTGCTGCGCTTTGCCCATGACAAGCCGAAACTGACGCGCTGGTCGGATAATGTGCGCATTCTCGAAGGGCTGGCGCAAAACGGCATCATGGAGGAGC AGGAAGCGGCAGGCATTGACGCTGGCGTACACCACATTGCGTGATGAGCTGCACCACCTGGCGCTGCAAGAGTTGCCGGGACATGTGGCGCTCTCCTGTTTTGTCGCCGAGCGTGCGCTTATTAAAACCAGCTGGGACAAGTGGCTGGTGGAACCGTGCGCCCCGGCGTAA

Assessing the convenience of gene manipulation

[0297] Microorganisms-candidates were characterized on the basis of the ability to transform and ease of manipulation of genes. First, the optimal utilization of the carbon source was determined by growing on a small panel of the appropriate medium, as well as the growth curve in nitrogen-free and rich media. Secondly, the natural antibiotic resistance of each strain was determined by the method of replication and growth in liquid culture containing a panel of antibiotics used as selective markers of mutagenesis. Third, each strain was tested for its ability to transform by electroporation of a collection of plasmids. The collection of plasmids contains a combinatorial extension of the seven origins of replication, i.e. p15a, pSC101, CloDF, colA, RK2, pBBR1 and pRO1600 and four antibiotic resistance markers, i.e. CmR, KmR, SpecR and TetR. This systemic compatibility assessment of origins of replication and resistance markers was used to identify vectors for plasmid-based mutagenesis of candidate microorganisms.

Example 3 Mutagenesis of Candidate Microorganisms

Mediated Red lambda knockout

[0298] Several candidate mutants were generated using the plasmid pKD46 or a derivative thereof containing a kanamycin resistance marker (Datsenko et al. 2000; PNAS 97(12): 6640-6645). Knockout cassettes were constructed with 250 bp homology flanking the target gene and generated by PCR with overlap completion. Candidate microorganisms were transformed with pKD46, cultured in the presence of arabinose to induce the Lambda Red expression mechanism, prepared for electroporation, and transformed with knockout cassettes to obtain candidate mutant strains. Four candidate microorganisms and one laboratory strain, Klebsiella oxytoca M5A1, were used to generate thirteen candidates mutated in the nitrogen fixation regulatory genes nifL , glnB , and amtB , as shown in Table 4 .

Strain nifL glnB amtB M5A1 X X X CI006 X X X CI010 X X X CI019 X X CI028 X X

Table 4: List of single knockout mutants generated using Red-mediated lambda mutagenesis

Oligonucleotide-specific mutagenesis with Cas9 selection

[0299] Oligonucleotide-specific mutagenesis was used to localize genome changes in the E. coli DH10B rpoB gene, and mutants were selected using the CRISPR-Cas system. A mutagenic oligonucleotide (ss1283: "G*T*T*G*ATCAGACCGATGTTCGGACCTTCcaagGTTTCGATCGGACATACGCGACCGTAGTGGGTCGGGTGTACGTCTCGAACTTCAAAGCC" (SEQ ID NO:2) , where * denotes a thiophosphate bond) was constructed to confer resistance to rifampicin via a 4 bp mutation. in the rpoB gene. Cells containing a plasmid encoding Cas9 were induced to express Cas9, prepared for electroporation, and then electroporated with a mutagenic oligonucleotide and a plasmid encoding constitutive expression of a guide RNA (nRNA) that directs Cas9 cleavage of the WT rpoB sequence. The electroporated cells were reconstituted in non-selective media overnight to ensure sufficient segregation of the resulting mutant chromosomes. After seeding on selection medium to select the coding nRNA plasmid, two of the ten colonies screened were shown to contain the desired mutation, while the rest were shown to be escape mutants resulting from a protospacer mutation in the nRNA plasmid or loss of the Cas9 plasmid. .

Red-mediated lambda mutagenesis with selection through Cas9

[0300] Received mutants of microorganism candidates CI006 and CI010 using Red-mediated lambda mutagenesis with selection by CRISPR-Cas. The knockout cassettes contained an endogenous promoter identified by transcriptome profiling (as described in Example 2 and shown in Tables 3A-C ) and ≈250 bp regions of homology flanking the deletion target. CI006 and CI010 were transformed with plasmids encoding the Red lambda recombination system (exo, beta, gam genes) under the control of an arabinose inducible promoter and Cas9 under the control of an IPTG inducible promoter. In the resulting transformants, the Red and Cas9 recombination systems were induced and strains for electroporation were obtained. Then knockout cassettes and a plasmid with encoded selection nRNA were transformed into competent cells. After seeding on selective media with antibiotics for Cas9 plasmid and nRNA plasmid, 7 out of 10 screened colonies showed the desired knockout mutation, as shown in Figure 3 .

Example 4: Phenotyping of Candidate Molecules in vitro

[0301] In various mutants, the effect of exogenous nitrogen on the biosynthesis and activity of nitrogenase was determined. Acetylene reduction assay (ARA) was used to determine nitrogenase activity under pure culture conditions (Temme et. al. 2012; 109(18): 7085-7090). The strains were grown in sealed test tubes and the reduction of acetylene to ethylene was quantified using an Agilent 6890 gas chromatograph. The activity of candidate microorganisms and other candidate mutants grown in nitrogen fixation media supplemented with 0 to 10 mM glutamine in ARA shown in figures 4A-B and figures 10A-C .

[0302] Under anaerobic culture conditions, a range of glutamine and ammonia concentrations was determined, and the effect on nitrogen fixation activity was quantified. In wild-type cells, as the concentration of glutamine increased, the activity rapidly decreased. However, a class of mutations has been confirmed in a series of parental knockout mutations that mediate the expression of nitrogen fixation genes under conditions of glutamine concentration, which would otherwise suppress this activity in the wild type. As seen in Figure 4C , this profile was obtained from four different diazotrophic species. In addition, by reconfiguring the regulatory network using the identified genetic components, the level of nitrogen fixation activity was regulated in a predictable manner. This can be observed in Figure 4B , which illustrates strains CM023, CM021, CM015 and CI006. Strain CM023 is a weakly evolved strain; strain CM021 is a highly evolved strain; strain CM015 is a medium evolved strain; strain CI006 is a wild-type strain (strain 2). Ammonia released into culture supernatants was tested using an enzymatic assay (MEGAZYME). The amount of consumed NADPH was determined in the assay at an optical density of 340 nm. The assay was performed in bacterial cultures grown in a nitrogen-free, anaerobic environment with an initial density of 10 ⁹ cfu/ml. As seen in Figure 4D , in a panel of six evolved strains, one of the strains released up to 100 μM ammonia over a 48 hour period. In addition, as seen in Figure 11 , the double mutant exhibited greater ammonia release than the single mutant from which it was derived. This demonstrates the ability of microorganisms to produce ammonia in quantities in excess of their physiological requirements.

Transcriptome profiling of pure cultures

[0303] Transcriptional activity of CI006 was determined using the Nanostring Elements platform. Cells were grown in a nitrogen-free medium and 10 ⁸ cells were harvested after 4 hours of incubation. Total RNA was isolated using the Qiagen RNeasy kit. The purified RNA was sent to Core Diagnostics in Palo Alto, CA for probe hybridization and Digital Analyzer analysis as shown in Figure 5 .

Example 5: Phenotyping of Candidate Microorganisms in planta

Colonization of plants by microorganism candidates

[0304] Colonization of desired host plants by candidate microorganisms was quantified through short term plant growth experiments. Maize plants were inoculated with strains expressing RFP from a plasmid or with a Tn5-integrated RFP expression cassette. Plants were grown in sterilized sand and non-sterile peat medium, and inoculation was carried out by applying 1 ml of cell culture with a pipette directly to the emerging plant coleoptile three days after germination. Plasmids were maintained by watering the plants with a solution containing the appropriate antibiotic. Three weeks later, plant roots were collected, washed three times in sterile water to remove visible soil, and divided into two samples. One root sample was analyzed by fluorescence microscopy to determine localization profiles of candidate microorganisms. Microscopy was performed on 10 mm parts of the best intact plant roots, as shown in figure 6 .

[0305] A second quantitative method for assessing colonization has been developed. A quantitative PCR analysis was carried out on preparations of total DNA from plant roots inoculated with endophytes. Corn seeds (Dekalb DKC-66-40) were germinated in pre-autoclaved sand in a 6.35 cm by 6.35 cm by 25.4 cm pot. location of seeds. 1 ml of this overnight culture is approximately equivalent to approximately 10 ⁹ cfu, with a variation within 3 times each other depending on the strain used. Each seedling was fertilized 3 times a week with 50 ml of modified Hoagland's solution supplemented with 2.5 mM or 0.25 mM ammonium nitrate. Four weeks after planting, root samples were collected for DNA extraction. Soil residues were washed off using a pressurized water spray. These tissue samples were then homogenized using the QIAGEN Tissuelyzer and then DNA was isolated using the QIAmp Mini DNA Isolation Kit (QIAGEN) following the recommended protocol. These isolated DNA samples were subjected to qPCR analysis using a Stratagene Mx3005P RT-PCR device using primers that are designed (using Primer BLAST NCBI) to be specific to the loci within each endophyte genome. The presence of copies of endophyte genomes was quantified. To further confirm the identification of endophytes, the sequencing of the PCR amplification products was performed and the presence of the correct sequence was confirmed. A summary of the colonization profiles of candidate microorganism strains CI006 and CI008 is presented in Table 5 . Strain CI008 demonstrated a colonization rate of up to 10 ⁷ × CFU/g ww of the root.

Strain Colonization Rate (cfu/g ww) CI006 1.45×10 ⁵ CI008 1.24×10 ⁷

Table 5: Maize colonization determined by qPCR

RNA profiling in planta

[0306] Biosynthesis of nif pathway components in planta was assessed by determining the transcription of nif genes. Total RNA was obtained from root tissues of plants inoculated with CI006 (planting methods described previously). RNA isolation was performed using the RNEasy mini kit according to the recommended protocol (QIAGEN). The total RNA of these plant tissues was then analyzed using Nanostring Elements kits (NanoString Technologies, Inc.) using probes specific for the nif genes in the CI006 strain genome. Data on nif gene expression in planta are summarized in Table 6 . Expression of nifH genes was detected in plants inoculated with strain CM013, while nifH expression was not detected in plants inoculated with CI006. Strain CM013 is a derivative of strain CI006 in which the nifL gene has been knocked out.

[0307] Highly expressed CM011 genes, ranked by transcripts in the so-called. per million (TPM), determined in planta under conditions of addition of fertilizers. Promoters controlling the expression of some of these highly expressed genes have been used as templates for homologous recombination at the target nitrogen fixation and assimilation loci. RNA samples from greenhouse-grown CM011 inoculated plants were isolated, rRNA removed using the Ribo-Zero kit, sequenced using the Truseq Illumina platform, and mapped back to the CM011 genome. Highly expressed CM011 genes are shown in Table 7 .

Strains Relative transcripts CI006 9.4 CM013 103.25

Table 6: Expression of nifH in planta

Gene name Gene position Direction Total number of reads TPM (transcripts in tons per million) CDS rpsH 18196-18588 reverse 4841.5 27206.4 CDS rplQ 11650-12039 reverse 4333 24536.2 CDS rpsJ 25013-25324 reverse 3423 24229 CDS rplV 21946-22278 reverse 3367.5 22333 CDS rpsN 18622-18927 reverse 2792 20150.1 CDS rplN 19820-20191 reverse 3317 19691.8 CDS rplF 17649-18182 reverse 4504.5 18628.9 CDS rpsD 13095-13715 reverse 5091.5 18106.6 CDS rpmF 8326-8493 direct 1363.5 17923.8 CDS rplW 23429-23731 reverse 2252 16413.8 CDS rpsM 14153-14509 reverse 2269 14036.2 CDS rplR 17286-17639 reverse 2243.5 13996.1 CDS rplC 24350-24979 reverse 3985 13969.2 CDS rplK 25526-25954 reverse 2648.5 13634.1 CDS rplP 20807-21217 reverse 2423 13019.5 CDS rplX 19495-19809 reverse 1824 12787.8 CDS rpsQ 20362-20616 reverse 1460.5 12648.7 CDS bhsA 3 79720-79977 reverse 1464 12531.5 CDS rpmC 20616-20807 reverse 998.5 11485 CDS rpoA 12080-13069 reverse 4855 10830.2 CDS rplD 23728-24333 reverse 2916.5 10628.5 CDS bhsA 1 78883-79140 reverse 1068 9141.9 CDS rpsS 22293-22571 reverse 1138.5 9011.8 CDS rpmA 2210-2467 direct 1028.5 8803.7 CDS rpmD 16585-16764 reverse 694.5 8520.8 CDS rplB 22586-23410 reverse 3132 8384 CDS rpsC 21230-21928 reverse 2574.5 8133.9 CDS rplE 18941-19480 reverse 1972.5 8066.9 CDS rplO 16147-16581 reverse 1551 7874.2 CDS of SecY translocase subunit precursor protein 14808-16139 reverse 4657 7721.2 CDS rpsE 16771-17271 reverse 1671.5 7368 CDS rpsK 13746-14135 reverse 1223.5 6928.2 CDS tufA 27318-28229 reverse 2850 6901.3 CDS rpmI 38574-38771 direct 615 6859.5 CDS rplU 1880-2191 direct 935.5 6621.7 CDS rplT 38814-39170 direct 1045 6464.4 CDS bhsA 2 79293-79550 reverse 754 6454.1 CDS rpmB 8391-8627 reverse 682 6355.1 CDS rplJ 23983-24480 reverse 1408 6243.9 CDS fusA 2 481-2595 reverse 5832 6089.6 CDS rpsA 25062-26771 reverse 4613 5957.6 CDS rpmJ 14658-14774 reverse 314 5926.9 CDS rpsR 52990-53217 direct 603 5840.7 CDS rpsG 2692-3162 reverse 1243 5828.2 CDS rpsI 11354-11746 reverse 980.5 5509.8 CDS cspC 1 8091-8300 reverse 509 5352.8 CDS rpsF 52270-52662 direct 916 5147.4 CDS rpsT 55208-55471 reverse 602 5035.9 CDS infC 38128-38478 direct 755 4750.3 CDS cspG 30148-30360 direct 446 4624.2

Table 7

Analysis ¹⁵ N

[0308] The main method for demonstrating fixation uses the nitrogen isotope ¹⁵ N, which is in the atmosphere at a set level relative to ¹⁴ N. By adding increased levels of ¹⁵ N to each fertilizer or atmosphere, fixation can be observed or directly in increased amounts of ¹⁵ N fixed from the atmosphere supplemented with ¹⁵ N ₂ gas (Yoshida 1980), or indirectly through dilution of enriched fertilizer with atmospheric N ₂ gas in plant tissues (Iniguez 2004). The dilution method allows observation of fixed nitrogen accumulation during plant growth, while the ¹⁵ N ₂ gas method is limited to determining fixation, which occurs over a short interval during which the plant can be grown in a confined atmosphere (velocity measurements). Thus, the gas method is better suited for determining specificity (since any elevated levels of ¹⁵ N ₂ in the plant above atmospheric levels can be unambiguously correlated with fixation), but they cannot show cumulative activity.

[0309] To determine the fixation activity of improved strains relative to wild-type and non-inoculated maize plants, both assays were performed and increased fixation levels were observed in several of the improved strains in planta ( Figure 12 , Figure 14A and Figure 14B ). These assays are important in demonstrating that the in vitro activity of test strains is consistent with in vivo results. In addition, these assays make it possible to determine the effect of a fertilizer on the activity of a strain, suggesting appropriate functionality under agricultural conditions. Similar results were observed when wild-type and improved strains were inoculated with Setaria plants ( Figure 13 ). In planta fixation activity shown in Figures 14A-14C was further confirmed by transcriptomic data. Evolved strains show an increased level of nifH transcripts relative to wild-type variants. In addition, the level of nitrogen provided by microorganisms in planta also correlated with the level of plant colonization per plant. These results ( Figure 12, Figure 13, Figures 14A-14C, Figure 15A and Figure 15B ) support the hypothesis that a microorganism is the likely cause of the increase in atmospheric nitrogen observed in plant tissues due to improved regulation of the nif gene cluster. In addition to direct determination of fixation, the effect of plant inoculation with improved strains was determined in a plant biomass analysis under nitrogen deficient conditions. Although plant biomass can be associated with a variety of possible microorganism-plant interactions, it would be expected that the addition of fixed nitrogen could influence plant phenotype under nitrogen-deficient conditions. The inoculated plants were grown under complete nitrogen-free conditions and a significant increase in leaf area, green and dry weight of shoots and wet and dry weight of roots was observed in the inoculated plants relative to untreated control plants ( Figure 14C ). Although these differences cannot be attributed solely to nitrogen fixation, they support the conclusion that improved strains actively provide nitrogen to plants. Corn and Setaria plants were grown and inoculated as described above. Fertilizer containing 1.2% ¹⁵ N was regularly added to plants by watering. Nitrogen fixation by microorganisms was quantified by determining the level of ¹⁵ N in plant tissue. Fourth leaf tissue was obtained at 4 weeks after planting and dried. Dried leaf samples were homogenized using pellets (QIAGEN Tissuelyzer) and aliquoted into IRMS tin capsules (MBL Stable Isotope Laboratory at The Ecosystems Center, Woods Hole, MA). Atmospheric nitrogen (NDFA) was calculated and nitrogen production of CI050 and CM002 is shown in Figure 7 .

Analysis of phytohormone production

[0310] Previously, the dwarf tomato ( Solanum lycopersicum ) cultivar "Micro-Tom" (Cohen 1996; J Am Soc Hortic Sci 121:520-524) was used to investigate the effect of indole-3-acetic acid on fruit ripening by in vitro assay. To assess the production and secretion of phytohormones by candidate microorganisms, a plate screening assay using immature Micro-Tom fruits was developed. Twelve-well tissue culture test plates were prepared by filling the wells with agarose medium, allowing it to solidify, and topping the agar surface with 10 µl of an overnight microorganism culture as shown in Figure 8 . Agar wells contain increased amounts of gibberellic acid (GA), but no bacterial cultures were used as positive controls and standards. Flowers cut from growing Micro-Tom plants one day after the start of flowering were placed in the agar in the area where the bacterial culture was applied stem first. These flowers were observed for 2-3 weeks, after which the fruits were collected and weighed. An increase in the mass of the plant fruit in several repetitions indicated the production of plant hormones by the inoculated microorganisms, as shown in figure 9 .

Example 6: Host-Microorganism Cyclic Evolution

[0311] Corn plants were inoculated with CM013 and grown for 4 weeks to approximately the V5 growth stage. Plants showing improved nitrogen uptake from microbial sources as measured by ¹⁵ N analysis were uprooted and the roots were washed using pressurized water to remove the underlying soil. A root cut was made weighing 0.25 g and washed in a PBS solution to remove small soil particles and unattached microorganisms. Tissue samples were homogenized using 3 mm steel beads in a QIAGEN TissueLyser II. The homogenate was diluted and plated on SOB agarose media. Single colonies were resuspended in liquid media and subjected to PCR analysis of 16s rDNA and mutations unique to the inoculated strain. The process of isolation of the microorganism, mutagenesis, inoculation and re-isolation can be repeated several times with the improvement of the characteristics of the microorganism, the characteristics of plants and the ability of microorganisms to colonize.

Example 7: Compatibility in different geographical conditions

[0312] The ability of the improved microorganisms to colonize the inoculated plant is critical to the success of the plant in the field. Although the isolation methods described are designed to select from soil microorganisms that may be closely related to crops such as corn, many strains cannot effectively colonize across a range of plant genotypes, environmental types, soil types, or inoculation conditions. Since colonization is a complex process that requires a number of interactions between a strain of microorganisms and a host plant, screening for the ability to colonize has become the main way to select priority strains for further development. Early attempts at assessing colonization used fluorescently labeled strains, which were effective but time consuming and did not scale across strains. Since colonization activity cannot be directly improved, it is critical to select potential production candidates from strains that are natural colonizers.

[0313] An assay was developed to test for stable colonization by wild-type strains of any given host plant using qPCR and primers designed to be specific for the strain in a community sample. This assay is designed to rapidly determine the rate of colonization of microorganisms from maize tissue samples. Initial tests using strains assessed as likely colonizers using fluorescence microscopy and plate methods demonstrated that the qPCR approach can be quantitative and scalable.

[0314] A typical analysis is carried out as follows: plants, mainly varieties of corn and wheat, are grown in peat potting mix in a greenhouse in six repetitions per strain. Four or five days after planting, 1 ml of the impregnation of cultures of bacteria in the early stationary phase, diluted to an OD ₅₉₀ of 0.6-1.0 (approximately 5×10 ⁸ CFU/ml), is pipetted onto an ascending coleoptile. The plants are irrigated with tap water only and left to rati for four weeks, followed by sampling, during which the plants are uprooted and the roots thoroughly washed to remove most of the peat residue. Samples of clean roots are cut out and homogenized to obtain a suspension of plant debris cells and associated bacterial cells. The authors have developed a protocol for high-throughput DNA isolation, which allows one to efficiently obtain a mixture of plant and bacterial DNA for use as a template for qPCR. Based on an experiment in a sharp increase in the number of bacterial cells, this method of DNA extraction obtains a quantitative sample of bacterial DNA relative to the wet weight of the roots. Each strain is evaluated using strain-specific primers designed using Primer BLAST (Ye 2012) and compared to background amplification in non-inoculated plants. Since some primers in non-inoculated plants showed side amplification, colonization is determined by the presence of amplification or increased amplification of the corresponding product compared to the background level.

[0315] This analysis was used to determine the compatibility of the product of microorganisms in different soil geographic conditions. Field soil qualities and field conditions can have a very large influence on the action of the microbial product. Soil pH, water retention capacity, and competing microorganisms are just a few examples of soil factors that can affect inoculum viability and colonization ability. Colonization analysis was performed using three different soil types sampled as plant growth media from agricultural fields in California ( Figure 16A ). An intermediate inoculation density was used to approximate real agricultural conditions. Within 3 weeks, strain 5 colonized all plants at a level of 10 ⁶ to 10 ⁷ cfu/g wet weight. After 7 weeks of plant growth, the evolved version of strain 1 showed high levels of colonization (10 ⁶ cfu/g wet weight) in all soil types. ( Figure 16B) .

[0316] In addition, to evaluate colonization in challenging field conditions in a 1-acre field in San Luis Obispo, a trial was initiated in June 2015 to evaluate the impact and colonization of seven wild-type strains on two varieties of field corn. Agrotechnical design and testing was contracted out to a field research organization, Pacific Ag Research. For inoculation, the same peat seed coating method was used that was tested in the inoculation method experiments. During the growing season, plant samples were collected to assess the colonization of the root and the interior of the stem. Samples were collected from three replicate plots after each treatment at four and eight weeks post planting, and from all six replicate sites after each treatment shortly before collection at 16 weeks. Additional samples were collected from all six replicate sites after strain 1 and strain 2 inoculation treatments, as well as untreated controls, 12 weeks later. The number of cells per gram wet weight of washed roots was evaluated as in other colonization assays using qPCR and strain-specific primers. Two strains, strain 1 and strain 2, showed stable and ubiquitous root colonization that peaked at 12 weeks and then declined sharply ( Figure 16C ). Although strain 2 appears to be present at an order of magnitude lower than strain 1, it has been found to be more constant in plant to plant amounts. Apparently, none of the strains effectively colonizes the interior of the stem. In support of the qPCR colonization data, both strains were successfully reisolated from root samples using plates and 16S sequencing to identify isolates of the respective sequence.

Example 8: Removal of microorganisms

[0317] Examples of breeding microorganisms can be summarized in the scheme of figure 17A . Figure 17A depicts microbial breeding, where the composition of the microbiome can first be determined and the species of interest identified. Microbiome metabolism can be mapped and linked to genetics. Directional genetic variation can then be carried out by methods including, but not limited to, conjugation and recombination, chemical mutagenesis, adaptive evolution, and gene editing. Derived microorganisms are used to inoculate crops. In certain examples, crops with the best phenotypes are selected.

[0318] As shown in Figure 17A, the composition of the microbiome can first be determined and the species of interest identified. Figure 17B is an expanded view of the microbiome determination step. Microbiome metabolism can be mapped and linked to genetics. Nitrogen metabolism may include the entry of ammonia (NH ₄ ⁺ ) from the rhizosphere into the bacterial cytosol via the AmtB transporter. Glutamine synthetase and ATP catalyze the conversion of ammonia and L-glutamate (L-Glu) to glutamine. Glutamine can lead to biomass formation (plant growth), and it can also inhibit the expression of the nif operon. Then, directed genetic variation can be carried out by methods including, but not limited to, conjugation and recombination, chemical mutagenesis, adaptive evolution, and gene editing. Derived microorganisms are used to inoculate crops. Selected crops with the best phenotypes.

Example 9: Field trials with microorganisms according to the invention - summer 2016

[0319] Field trials were conducted to evaluate the effect of the strains of the present invention on corn growth and productivity under various nitrogen regimes.

[0320] The tests were performed with (1) treatment of seven sites with six strains and control - four main sites were set to 0, 15, 85 and 100% maximum nitrogen return (MRTN) with regional confirmation. Control (UTC only) was run at 10 100% MRTN plus 5, 10, or 15 lbs. Treatments were performed in four repetitions.

[0321] Plots of corn (minimum) were 4 rows of 9.144 meters long with 124 plots per region. All observations were made in the central two rows of plots, and all sample preparations with destruction were made in the outer rows. Seed samples were cooled to 1.5-2 hours before use.

[0322] Regional agricultural practice: Seeds were commercial corn without conventional fungicide and insecticide treatments. All seed treatments were performed by a single seed treatment specialist to ensure uniformity. Planting date, seeding rate, weed/insect control, etc. left as in regional agricultural practice. With the exception of the use of fungicides, standard methods of regulation were used.

[0323] Soil characteristics: Soil texture and soil fertility were assessed. Soil samples for each iteration were pre-seeded to ensure residual nitrate levels below 22.7 kg/acre. Soil samples were obtained from 0 cm to 30 cm. The soil was further characterized by pH, CEC, total K and P.

[0324] Assessments: Initial plant population was assessed 14 days after planting (DAP)/acre and further assessed for: (1) power (on a scale of 1 to 10, w/10=excellent) 14 DAP &V10; (2) keeping a record of disease ratings at any time the sites show symptoms; (3) record any differences in lodging if lodging occurs at the site; (4) yield (Bu/acre) corrected for standard percentage moisture; (5) the weight of the dough; and (6) the moisture percentage of the grains.

[0325] Requirements for obtaining samples: A soil sample was obtained at three time points (before the test, V10-VT, 1 week after harvest). All six regions and all regions were sampled at 10 grams per sample (124 regions x 3 time points x 6 regions).

[0326] Colonization sample preparation : Colonization samples were collected at two time points (V10 and VT) for five regions and six time points (V4, V8, V10, VT, R5 and post-harvest). Samples were collected as follows: (1) from 0% and 100% MRTN, 60 plots per region; (2) 4 plants per plot randomly selected from the outer rows; (3) 5 grams of root, 20.3 cm stem and top three leaves, each individually bagged and assigned an ID of 12/bags per lot; (4) five regions (60 plots × 2 time points × 12 bags/plot); and one region (60 plots x 6 time points x 12 bags/plot. See Figure 17C illustrating the preparation of colonization samples.

[0327] The determination of the normalized difference vegetation index (NDVI) was performed using the Greenseeker tool at two time points (V4 - V6 and VT). Each site was analyzed in all six regions (124 sites x 2 time points x 6 regions).

[0328] Root analysis was performed using Win Rhizo from the region that best illustrated processing differences. Ten plants per plot were randomly selected (5 near each outer row; plants were preferably selected at V3-V4) and washed gently to remove as much contaminants as possible. Ten roots were placed in a plastic bag and labelled. Root analysis was performed using WinRhizo root assay.

[0329] Stem characteristics were performed for all six regions between R2 and R5. The stem diameter of ten plants per plot at a height of 15 cm was recorded, as was the length of the first internode above the 15 cm mark. Ten plants were monitored; five consecutive plants from the center of the two inner rows. Six regions were evaluated (124 sites × 2 measurements × 6 regions).

[0330] Tissue nitrates were analyzed at all sites and in all regions. Stem section 20.3 cm long starting 15 cm above the soil when corn was between the first and third weeks after blackpoint formation; leaf sheaths were removed. All regions and sites were evaluated (6 regions × 124 sites).

[0331] For all regions from planting to harvest, the following weather data was recorded: daily maximum and minimum temperatures, soil temperature at planting, daily precipitation plus watering (if applicable), and any unusual weather events such as heavy rain, wind, cold or fever.

[0332] Table 8 shows yield data for all six regions. The level of nitrogen significantly affected the yield, but the strains had no effect on nitrogen levels. However, strains CI006, CM029 and CI019 outnumbered UTC by 4-6 bu/acre at the lowest nitrogen levels. Yields also increased numerically by 2-4 bu/acre with strains CM029, CI019 and CM081 at 15% MRTN.

Table 8: Yield data for all six regions

MRTN% Yield (bu) Power _E Power _L Stem diameter (mm) Internode length (in) NDVI_Veg NDVI_Rep 0 143.9 7.0 5.7 18.87 7.18 64.0 70.6 15 165.9 7.2 6.3 19.27 7.28 65.8 72.5 85 196.6 7.1 7.1 20.00 7.31 67.1 74.3 100 197.3 7.2 7.2 20.23 7.37 66.3 72.4 Strain YLD (bu) Power _E Power _L Stem diameter (mm) Internode length (in) NDVI_Veg NDVI_Rep CI006 (1) 176.6 7.2 6.6 19.56 18.78 66.1 72.3 CM029 (2) 176.5 7.1 6.5 19.54 18.61 65.4 71.9 CM038(3) 175.5 7.2 6.5 19.58 18.69 65.7 72.8 CI019 (4) 176.0 7.1 6.6 19.51 18.69 65.5 72.9 CM081 (5) 176.2 7.1 6.6 19.57 18.69 65.8 73.1 CM029/CM081 (6) 174.3 7.1 6.6 19.83 18.79 66.2 72.5 UTC (7) 176.4 7.1 6.6 19.54 18.71 65.9 71.7 MRTN/Strain YLD (bu) Power _E Power _L Stem diameter (mm) Internode length (in) NDVI_Veg NDVI_Rep 0 1 145.6 7.0 5.6 19.07 7.12 63.5 70.3 0 2 147.0 7.0 5.5 18.74 7.16 64.4 70.4 0 3 143.9 7.0 5.5 18.83 7.37 64.6 70.5 0 4 146.0 6.9 5.7 18.86 7.15 63.4 70.7 0 5 141.7 7.0 5.8 18.82 7.05 63.6 70.9 06 142.2 7.2 5.8 19.12 7.09 64.7 69.9 0 7 141.2 7.0 5.8 18.64 7.32 64.0 71.4 15 1 164.2 7.3 6.1 19.09 7.21 66.1 71.5 15 2 167.3 7.2 6.3 19.32 7.29 65.5 72.7 15 3 165.6 7.3 6.3 19.36 7.23 64.8 72.5 15 4 167.9 7.3 6.4 19.31 7.51 66.1 72.3 15 5 169.3 7.2 6.2 19.05 7.32 66.0 72.8 15 6 161.9 7.1 6.3 19.45 7.20 66.2 72.2 15 7 165.1 7.3 6.4 19.30 7.18 66.0 73.3 85 1 199.4 7.3 7.2 19.70 7.32 67.2 74.0 85 2 195.1 7.1 7.2 19.99 7.09 66.5 74.4 85 3 195.0 7.0 7.0 20.05 7.26 67.3 74.6 85 4 195.6 7.2 7.1 20.04 7.29 66.4 74.4 85 5 196.4 7.2 7.0 19.87 7.39 67.3 74.5 85 6 195.1 7.0 6.9 20.35 7.34 67.4 74.4 85 7 199.5 6.9 7.2 19.97 7.48 67.4 74.1 100 1 197.1 7.2 7.3 20.38 7.68 67.5 73.4 100 2 196.5 7.0 7.1 20.11 7.21 65.3 70.2 100 3 197.6 7.5 7.3 20.08 7.42 66.3 73.4 100 4 194.6 7.1 7.1 19.83 7.40 66.1 74.1 100 5 197.4 7.2 7.3 20.53 7.36 66.2 74.3 100 6 198.1 7.2 7.4 20.40 7.16 66.6 73.6 100 7 199.9 7.2 7.2 20.26 7.32 66.2 68.1

[0333] Table 9 presents a different approach to the analysis. The table shows the four regions where the response to nitrogen was greatest, suggesting that available residual nitrogen was the lowest. This approach does not change the assessment that nitrogen levels significantly affect yield, which strains did not do when averaged over all nitrogen levels. However, the numerical yield advantage at the lowest N level is more pronounced for all strains, especially for CI006, CM029 and CM029/CM081 where yields increased from 8 to 10 bu/acre. At 15% MRTN, strain CM081 outperformed UTC by 5 bu.

Table 9: Yield data for four regions

Average of 4 regions - SGS, AgIdea, Bennett, RFR Table 16 MRTN% YLD (bu) Power _E Power _L Stem diameter (mm) Internode length (in) 0 137.8 7.3 5.84 18.10 5.36 15 162.1 7.5 6.63 18.75 5.40 85 199.2 7.4 7.93 19.58 5.62 100 203.5 7.5 8.14 19.83 5.65 Strain YLD (bu) Power _E Power _L Stem diameter (mm) Internode length (in) CI006 (1) 175.4 7.5 7.08 19.03 5.59 CM029 (2) 176.1 7.4 7.08 19.09 5.39 CM038(3) 175.3 7.5 7.05 19.01 5.59 CI019 (4) 174.8 7.5 7.16 19.02 5.45 CM081 (5) 176.7 7.4 7.16 19.00 5.53 CM029/CM081 (6) 175.1 7.4 7.17 19.33 5.46 UTC (7) 176.0 7.3 7.27 18.98 5.55 MRTN/Strain YLD (bu) Power _E Power _L Stem diameter (mm) Internode length (in) 0 1 140.0 7.3 5.69 18.32 5.28 0 2 140.7 7.4 5.69 18.19 5.23 0 3 135.5 7.3 5.63 17.95 5.50 0 4 138.8 7.3 5.81 17.99 5.36 0 5 136.3 7.3 6.06 18.05 5.34 06 141.4 7.5 6.00 18.43 5.30 0 7 131.9 7.3 6.00 17.75 5.48 15 1 158.0 7.6 6.44 18.53 5.34 15 2 164.1 7.5 6.56 19.13 5.42 15 3 164.3 7.6 6.63 18.68 5.51 15 4 163.5 7.6 6.81 18.84 5.34 15 5 166.8 7.5 6.63 18.60 5.39 15 6 156.6 7.4 6.56 18.86 5.41 15 7 161.3 7.5 6.81 18.62 5.42 85 1 199.4 7.6 8.00 19.15 5.63 85 2 199.0 7.4 8.09 19.49 5.46 85 3 198.2 7.4 7.75 19.88 5.69 85 4 196.8 7.4 8.00 19.65 5.60 85 5 199.5 7.4 7.75 19.26 5.70 85 6 198.7 7.3 7.81 19.99 5.61 85 7 202.8 7.2 8.13 19.66 5.65 100 1 204.3 7.4 8.19 20.11 6.10 100 2 200.6 7.3 8.00 19.53 5.46 100 3 203.3 7.7 8.19 19.55 5.67 100 4 200.2 7.6 8.00 19.59 5.49 100 5 203.9 7.4 8.19 20.08 5.68 100 6 203.8 7.5 8.31 20.05 5.52 100 7 208.1 7.4 8.13 19.90 5.63

[0334] The results of field trials are also illustrated in Figures 21-27 . The results indicate that the microorganisms according to the invention are able to increase the yield of plants, which indicates the ability of the studied microorganisms to increase nitrogen fixation in an important agricultural crop, i. corn.

[0335] Field results further support the described methods for non-intergeneric modification of the genome of selected strains of microorganisms to obtain agriculturally significant results in the field when these engineered strains are applied to a crop.

[0336] Figure 18 shows a line of modified strains derived from strain CI006 (wild type Kosakonia sacchari ). Field data demonstrate that a genetically engineered derivative of wild-type CI006 strains, i. CM029, can lead to numerically relevant results in the field. For example, Table 8 shows that at 0% MRTN, CM029 resulted in a yield of 147.0 bu/acre compared to the untreated control at 141.2 bu/acre (an increase of 5.8 bu/acre). Table 8 also shows that at 15% MRTN CM029 resulted in a yield of 167.3 bu/acre compared to the untreated control at 165.1 bu/acre (an increase of 2.2 bu/acre). Table 9 supports these conclusions and demonstrates that at 0% MRTN CM029 resulted in a yield of 140.7 bu/acre compared to the untreated control at 131.9 bu/acre (an increase of 8.8 bu/acre). Table 9 also shows that at 15% MRTN CM029 resulted in a yield of 164.1 bu/acre compared to the untreated control at 161.3 bu/acre (an increase of 2.8 bu/acre).

[0337] Figure 19 shows a line of modified strains derived from strain CI019 (wild type Rahnella aquatilis ). Field data demonstrate that a genetically engineered derivative of wild-type strains of CI019, i. CM081, can lead to numerically relevant results in the field. For example, Table 8 shows that at 15% MRTN CM081 resulted in a yield of 169.3 bu/acre compared to the untreated control at 165.1 bu/acre (an increase of 4.2 bu/acre). Table 9 supports these conclusions and demonstrates that at 0% MRTN CM081 resulted in a yield of 136.3 bu/acre compared to the untreated control at 131.9 bu/acre (an increase of 4.4 bu/acre). Table 9 also shows that at 15% MRTN CM081 resulted in a yield of 166.8 bu/acre compared to the untreated control at 161.3 bu/acre (an increase of 5.5 bu/acre).

[0338] In addition, it can be observed in Table 9 that the combination of CM029/CM081 at 0% MRTN resulted in a yield of 141.4 bu/acre compared to the untreated control at 131.9 bu/acre (an increase of 9.5 bu/acre). acre ).

Example 10: Field trials with microorganisms according to the invention

[0339] In nature, including agricultural soils, a wide variety of nitrogen-fixing bacteria can be found. However, the microorganism's potential to provide sufficient nitrogen to crops to ensure reduced fertilizer use may be limited by suppression of nitrogenase genes in fertilized soils, as well as a low amount in close association with crop roots. Identification, isolation and breeding of microorganisms that are closely associated with key commercial crops can disrupt and improve the regulatory networks linking nitrogen sensitivity and nitrogen fixation and unlock the significant contribution of crop associated microorganisms to nitrogen levels. For this purpose, nitrogen-fixing microorganisms associated with and colonizing the root system of maize have been identified.

[0340] Root samples of corn plants grown in agronomically significant soils were collected, and populations of microorganisms were isolated from the rhizosphere and endosphere. The genomic DNA of these samples was isolated followed by sequencing of the 16S amplicons to profile the composition of the community. The microorganism Kosakonia sacchari (strain PBC6.1) was isolated and classified by 16S rRNA and whole genome sequencing. It is a particularly interesting nitrogen fixer, capable of colonizing nearly 21% of the root-associated microbiota (FIG. 30). To assess strain sensitivity to exogenous nitrogen, levels of nitrogen fixation in pure culture were determined using the classical acetylene reduction assay (ARA) and various levels of glutamine supplementation. The species showed a high level of nitrogen fixation activity in nitrogen-free environments, but exogenous fixed nitrogen suppressed nif gene expression and nitrogenase activity (strain PBC6.1, figures 28C and 28D). In addition, when the ammonia released in the supernatant of PBC6.1 grown under nitrogen fixing conditions was determined, very little release of fixed nitrogen could be detected (FIG. 28E).

[0341] The authors hypothesized that PBC6.1 could become a significant source of fixed nitrogen in fertilized fields if the regulatory networks controlling nitrogen metabolism were redesigned to ensure optimal nitrogenase expression and ammonia release in the presence of fixed nitrogen. Sufficient genetic diversity must exist in the PBC6.1 genome to allow for a broad phenotypic reconstruction without insertions of transgenes or synthetic regulatory elements. The genome of the isolated strain is at least 5.4 kb. and contains the canonical cluster of nitrogen fixation genes. The associated nitrogen metabolism pathways in PBC6.1 are similar to those of the nitrogen fixation model organism, Klebsiella oxytoca m5al .

[0342] Several nodes of the gene regulatory network have been identified that can enhance nitrogen fixation and its subsequent transfer to the host plant, especially under conditions of high exogenous concentrations of fixed nitrogen (Fig. 28A). The nifLA operon directly regulates the rest of the nif cluster through transcriptional activation by NifA and nitrogen- and oxygen-dependent repression of NifA by NifL. Destruction of nifL can abolish NifA inhibition and improve nif expression in the presence of oxygen and exogenous fixed nitrogen. In addition, the expression of nifA under the control of a nitrogen-independent promoter can separate nitrogenase biosynthesis from regulation by the nitrogen-sensitive NtrB/NtrC complex. The assimilation of fixed nitrogen by the microorganism into glutamine via glutamine synthetase (GS) is reversibly regulated by the two-domain enzyme adenylate transferase (ATase) GlnE through adenylation and deadenylation of GS with attenuation and restoration of activity, respectively. Shortening of the GlnE protein with the removal of its adenylate-removing (AR) domain can lead to a constitutively adenylated glutamine synthetase, which limits the assimilation of ammonia by the microorganism and increases the content of intra- and extracellular ammonia. Finally, a decrease in the expression of AmtB, the carrier responsible for ammonia uptake, can lead to an increase in extracellular ammonia. To obtain rationally constructed phenotypes of microorganisms without the use of transgenes, two approaches were used: obtaining marker-free deletions of genomic sequences encoding protein domains or entire genes, and rearranging regulatory networks through intragenomic rearrangement of promoters. Several non-transgenic derivatives of PBC6.1 strains were obtained through a repetitive mutagenesis process (Table 10).

Table 10. List of isolated and derived strains of K. sacchari used in this work. Prm, promoter sequence derived from the PBC6.1 genome; ΔglnE _AR 1 and ΔglnE _AR 2, various truncated variants of the glnE gene sequence that removes the adenylate-removing domain.

Strain ID Genotype PBC6.1 wild type PBC6.14 ΔnifL::Prm1 PBC6.15 ΔnifL::Prm5 PBC6.22 ΔnifL::Prm3 PBC6.37 ∆nifL::Prm1 ∆glnE _AR 2 PBC6.38 ∆nifL::Prm1 ∆glnE _AR 1 PBC6.93 ΔnifL::Prm1 ΔglnE _AR 2 ΔamtB PBC6.94 ∆nifL::Prm1 ∆glnE _AR 1 ∆amtB

[0343] Several in vitro assays have been performed to characterize specific phenotypes of derived strains. To assess the sensitivity of the strain to exogenous nitrogen used ARA, in which PBC6.1 showed suppression of nitrogenase activity at high concentrations of glutamine (Fig. 28D). In contrast, most derived strains showed a derepressed phenotype with varying levels of acetylene reduction observed at high glutamine concentrations. The transcription rates of nifA in samples analyzed by qPCR correlated well with the rates of acetylene reduction (Fig. 31), supporting the hypothesis that disruption of nifL and insertion of a nitrogen-independent promoter to control nifA may lead to derepression of the nif cassette. Strains with altered GlnE or AmtB activity showed markedly increased levels of ammonium excretion compared to wild-type or derivative strains without these mutations (FIG. 28E), illustrating the effect of these genotypes on ammonia assimilation and reuptake.

[0344] Two experiments were conducted to study the interaction of PBC6.1 derivatives with corn plants and to quantify the introduction of fixed nitrogen into plant tissues. First, in a greenhouse study, levels of nitrogen fixation by microorganisms were quantified using isotope tracers. Briefly, plants are grown with ¹⁵ N labeled fertilizer, and dilute concentrations of ¹⁵ N in plant tissues indicate the contribution of fixed nitrogen from microorganisms. Corn seedlings were inoculated with the selected microbial strains and the plants were grown to the V6 growth stage. The plants were then destroyed to allow the determination of microorganism colonization and gene expression, as well as the measurement of ¹⁵ N/ ¹⁴ N ratios in plant tissues by isotope ratio mass spectrometry (IRMS). Exposed tissue analysis demonstrated a small non-significant contribution of PBC6.38 and a significant contribution of PBC6.94 (p=0.011) to nitrogen levels in plants. Approximately 20% of the nitrogen found in aboveground maize leaves is produced by PBC6.94, with the remainder coming from seed, soil mix, or “background” fixation by other soil microorganisms (Figure 29C). This demonstrates that the inventors' microbial propagation pipeline can produce strains capable of making a significant contribution of nitrogen to plants in the presence of nitrogen fertilizers. Transcription of microorganisms in plant tissues was determined, and expression of the nif gene cluster was observed in derived strains but not in the wild-type strain (Fig. 29B), demonstrating the importance of nif derepression for the contribution of BNF to crops under fertilization conditions. Root colonization determined by qPCR showed that each of the strains tested had a different colonization density (FIG. 29A). PBC6.38 and PBC6.94 showed a 50-fold difference in colonization. This difference may indicate that PBC6.94 has reduced rhizosphere fitness compared to PBC6.38 as a result of high levels of fixation and excretion.

Ways

environments

[0345] The minimum medium contains (per liter) 25 g Na ₂ HPO ₄ , 0.1 g CaCL ₂ ⋅2H ₂ O, 3 g KH ₂ PO ₄ , 0.25 g MgSO ₄ ⋅7H ₂ O, 1 g NaC1, 2.9 mg FeCl ₃ , 0.25 mg Na ₂ MoO ₄ ⋅2H ₂ O and 20 g sucrose. Growth medium is defined as minimal medium supplemented with 50 ml of 200 mM glutamine per liter.

Isolation of diazotrophs

[0346] Corn seedlings were grown from seeds (DKC 66-40, DeKalb, IL) for two weeks in greenhouse conditions, controlled from 22°C (night) to 26°C (day), and provided with 16 hour light cycles in soil collected in San Joaquin County, CA. The roots were harvested and washed with sterile deionized water, removing the main soil. Root tissues were homogenized with 2 mm stainless steel balls in a tissue lyser (TissueLyser II, Qiagen P/N 85300) for three minutes at setting 30 and samples were centrifuged for 1 minute at 13,000 rpm to separate tissue from bacteria associated with the root. The supernatants were divided into two fractions and one was used for microbiome characterization by 16S rRNA amplicon sequencing, and the remaining fraction was diluted and plated on nitrogen free (NfB) medium supplemented with 1.5% agar. The plates were incubated at 30°C for 5-7 days. The resulting colonies were tested for the presence of the nifH gene by colony PCR with primers Ueda19f and Ueda406r. Genomic DNA of nifH positive colony PCR strains (QIAamp DNA Mini Kit, Cat No. 51306, QIAGEN, Germany) was isolated and sequenced (Illumina MiSeq v3, SeqMatic, Fremont, CA). After assembly and sequence annotation, isolates containing nitrogen fixation gene clusters were used in subsequent studies.

Profiling the microbiome from seedlings after isolation

[0347] Genomic DNA was isolated from root associated bacteria using the ZR-96 Genomic DNA I kit (Zymo Research P/N D3011) and 16S rRNA amplicons were generated using Nextera barcoded primers directed to 799f and 1114r. Amplicon libraries were purified and sequenced using the Illumina MiSeq v3 platform (SeqMatic, Fremont, CA). Readings were classified by taxa using Kraken using the minikraken database (Figure 30).

Acetylene Recovery Analysis (ARA)

[0348] A modified version of the acetylene reduction assay was used to determine nitrogenase activity under pure culture conditions. Strains were grown from a single colony in SOB (RPI, P/N S25040-1000) at 30°C with agitation at 200 rpm for 24 hours, and then subcultured 1:25 into growth medium and aerobically grown for 24 hours (30°C, 200 rpm). Then 1 ml culture from minimal medium was added to 4 ml minimal medium supplemented with 0 to 10 mM glutamine in airtight Hungate tubes and grown anaerobically for 4 hours (30° C., 200 rpm). Removed 10% headspace, then by injection carried out the replacement with an equal volume of acetylene, and the incubation was continued for 1 hour. 2 ml of head space was then removed with a gas-tight product quantification syringe to quantify ethylene production using an Agilent 6850 gas chromatograph equipped with a flame ionization detector (FID).

Ammonium release analysis

[0349] The excretion of fixed nitrogen in the form of ammonia was determined using batch fermentation in anaerobic bioreactors. Strains were grown from a single colony in 1 ml/well SOB in 96-well DeepWell plates. The plates were incubated at 30° C. with shaking at 200 rpm for 24 hours and then diluted 1:25 in a fresh plate containing 1 ml/well of growth medium. Cells were incubated for 24 hours (30°C, 200 rpm) and then diluted 1:10 in fresh plate containing minimal medium. The plate was transferred to an anaerobic chamber with a gas mixture of >98.5% nitrogen, 1.2-1.5% hydrogen and <30 ppm. oxygen and incubated at 1350 rpm, room temperature for 66-70 hours. The biomass of the original culture was compared with the final biomass by determining the optical density at 590 nm. The cells were then separated by centrifugation and the supernatant from the reaction broth was analyzed for free ammonia using the Megazyme Ammonia Assay Kit (P/N K-AMIAR) normalized to biomass at each time point.

Isolation of the root-associated microbiome

[0350] The roots were gently shaken to remove loose particles and the root systems were separated and soaked in RNA stabilizing solution (Thermo Fisher P/N AM7021) for 30 minutes. The roots were then washed briefly with sterile deionized water. Samples were homogenized using ball agitation using 1.27 cm stainless steel ball bearings in a tissue lyser (TissueLyser II, Qiagen P/N 85300) in 2 ml lysis buffer (Qiagen P/N 79216). Genomic DNA isolation was performed using the ZR-96 Quick-gDNA kit (Zymo Research P/N D3010) and RNA extraction was performed using the RNeasy kit (Qiagen P/N 74104).

Root colonization assay

[0351] Four days after planting, 1 ml of bacterial overnight culture (approximately 10 ⁹ CFU) was applied to the soil above the planted seeds. The seedlings were fertilized three times a week with 25 ml of Hoagland's modified solution supplemented with 0.5 mM ammonium nitrate. Four weeks after planting, root samples were collected and total genomic DNA (gDNA) was isolated. Root colonization was quantified using qPCR with primers designed to amplify unique regions of the wild type or derived strain genomes. The efficiency of the qPCR reaction was determined using a standard curve derived from known amounts of gDNA from the target genome. The data were normalized to the number of genome copies per gram wet weight using tissue weight and isolation volume. For each experiment, the number of colonizations was compared to untreated control seedlings.

Transcriptomics in planta

[0352] Transcriptional profiling of root-associated microorganisms was performed on seedlings grown and treated as described in the Root Colonization Assay section. Purified RNA was sequenced using the Illumina NextSeq platform (SeqMatic, Fremont, CA). Reads were mapped to the genome of the inoculated strain using bowtie2 using the '-very-sensitive-local' parameters and a minimum alignment score of 30. Genome coverage was calculated using samtools. Differential coverage was normalized for housekeeping gene expression and visualized across the genome using Circos and across the nif gene cluster using DNAplotlib. In addition, the in planta transcriptional profile was quantified by targeted Nanostring analysis. Purified RNAs were processed on nCounter Sprint (Core Diagnostics, Hayward, CA).

Greenhouse breeding research ¹⁵ N

[0353] A fertilizer dilution experiment with ¹⁵ N was performed to evaluate the in planta activity of the optimized strain. Planting media containing minimal background N was prepared using a mixture of vermiculite and washed sand (5 washes in DI H ₂ O). The sand mixture was autoclaved for 1 hour at 122°C and measured approximately 600 g in 656 ml pots, which were soaked in sterile DI H ₂ O and left to drain 24 hours before planting. The surface of corn seeds (DKC 66-40) was sterilized in 0.625% sodium hypochlorite for 10 minutes, then washed five times in sterile distilled water and planted at a depth of 1 cm. The plants were kept under fluorescent lamps for four weeks with a 16 hour day length at room temperatures average between 22°C (night) and 26°C (day).

[0354] Five days after planting, seedlings were inoculated with 1 ml of cell suspension by soaking directly over the growing coleoptile. The inoculum was prepared from a 5 ml overnight culture in SOB, which was centrifuged twice and resuspended in 5 ml PBS to remove residual SOB followed by a final dilution to OD 1.0 (approximately 10 ⁹ CFU/ml). Control plants were treated with sterile PBS and each treatment was for ten plant replicates.

[0355] Plants were fertilized with 25 ml of a fertilizer solution containing 2% enriched in ¹⁵ N 2 mm KNO ₃ on days 5, 9, 14 and 19 after planting, and the same solution without KNO ₃ on days 7, 12, 16 and 18 after landing. The fertilizer solution contained (per litre) 3 mmol CaCl ₂ , 0.5 mmol KH ₂ PO ₄ , 2 mmol MgSO ₄ , 17.9 µmol FeSO ₄ , 2.86 mg H ₃ BO ₃ , 1.81 mg MnCl ₂ ⋅4H ₂ O, 0.22 mg ZnSO ₄ ⋅7H ₂ O, 51 µg CuSO ₄ ⋅5H ₂ O, 0.12 mg Na ₂ MoO ₄ ⋅2H ₂ O and 0.14 nmol NiCl ₂ . All pots were wetted as needed with sterile DI H ₂ O to maintain proper soil moisture without dripping.

[0356] Four weeks later, plants were harvested and separated at the lowest node into samples for root gDNA and RNA isolation and exposed tissues for IRMS. The exposed fabrics were wiped to remove sand as needed, placed whole in paper bags and dried for at least 72 hours at 60°C. After complete drying, the total exposed tissue was homogenized by mixing with the beads and 5-7 mg samples were analyzed by isotope ratio mass spectrometry (IRMS) for δ15N at the MBL Stable Isotope Laboratory (The Ecosystems Center, Woods Hole, MA). The percentage of NDFA was calculated using the following formula: %NDFA=(δ15N mean UTC - δ15N sample)/(δ15N mean UTC) × 100.

Example 11: Field trials with microorganisms according to the invention - summer 2017

[0357] To evaluate the impact of the strains of the present invention, the growth and productivity of corn under various nitrogen regimes were conducted field trials. The following field data demonstrates that the non-intergeneric microorganisms of the invention are capable of fixing atmospheric nitrogen and delivering said nitrogen to the plant, resulting in increased yields in nitrogen-limited as well as non-nitrogen-limited environments.

[0358] Tests were conducted in seven regions of the United States in six geographically distinct midwestern regions. Five nitrogen regimens were used for fertilization: 100% site/region standard agricultural practice, 100% minus 25 lbs, 100% minus 50 lbs, 100% sine 75 lbs, and 0%; all data are per acre. Pounds of nitrogen per acre for the 100% regime was dependent on site/region standard farming practices. The above nitrogen regimens ranged from about 153 pounds per acre to about 180 pounds per acre with an average of about 164 pounds of nitrogen per acre.

[0359] For each fertilizer regimen, 14 treatments were performed. Six repetitions were performed for each regimen, and a split-plot scheme was used. The 14 treatments included: 12 different microorganisms, 1 UTC with the same level of fertilizer as the main plot, and 1 UTC with 100% nitrogen. In 100% nitrogen mode, 2nd UTC was 100 plus 25 pounds.

[0360] Plots of corn, at a minimum, consisted of 4 rows of 9.144 m in length (76.2 cm between rows) with 420 plots per region. Unless otherwise indicated, all observations were made in the center two rows of plots, and all sample preparations with destruction were made in the outer rows. Seed samples were cooled to 1.5-2 hours before use.

[0361] Regional agricultural practice: Seeds were commercial corn used with commercial seed treatment without biological co-application. Seeding rate, planting date, weed/insect control, harvest times, and other standard farming practices were in line with local agricultural practices for the regions, with the exception of fungicide application (if required).

[0362] Application of Microorganisms: Microorganisms were applied to the seeds in a seed treatment for seeds that had already undergone normal chemical treatment. The seeds were covered with a fermentation broth containing microorganisms.

[0363] Soil characteristics: Soil texture and soil fertility were assessed. Standard soil sampling methods were used, which included soil samples at depths of 0-30 cm and 30-60 cm. The soil sampling standard included determination of nitrate nitrogen, ammonium nitrogen, total nitrogen, organic matter, and CEC. The standard for obtaining a soil sample additionally included the determination of pH, total potassium and total phosphorus. To determine nitrogen fertilizer levels, pre-plant soil samples were obtained from each region to ensure that 0-12" and potentially 12" to 24" soil regions for nitrate nitrogen.

[0364] Before planting and fertilizing, 2 ml of soil samples were collected from 0 to 6-12" from UTC. Collected one sample per repetition per nitrogen area using the middle of the row. (5 fertilizer regimens × 6 repetitions = thirty soil samples) .

[0365] After planting (V4-V6), 2 ml of soil samples from 0 to 6-12" from UTC were collected. One sample was collected per repeat for each nitrogen area using the middle of the row. (5 fertilization regimens × 6 repetitions = thirty soil samples).

[0366] After harvesting (V4-V6), 2 ml soil samples were collected from 0 to 6-12" from UTC. Collected one sample per repetition for each nitrogen area using the middle of the row. 0-12" from UTC and potentially 12-24" from UTC (5 fertilizer applications x 6 repetitions = thirty soil samples).

[0367] Soil sample for V6-V10 for each fertilizer setting (excluding 100% and 100%+25 lb treatment [in 100% block] for all fertilizer settings at 0-12" and 12-24" (5 fertilizer settings × 2 depths=10 samples per region).

[0368] After harvest, soil sample for each fertilization regimen (excluding 100% and 100%+25 lb treatment [in 100% block] for all fertilization regimens at 0-12" and 12-24". (5 fertilization regimens × 2 depth=10 samples per region).

[0369] Analysis: The initial plant population was evaluated at ≈50% UTC, and the final plant population was evaluated before harvest. The analysis included (1) potential temperature (temperature sensor); (2) power (on a scale of 1-10 with 10=excellent) at V4 and V8-V10; (3) plant height at V8-V10 and V14; (4) yield (bushels/acre) corrected for standard moisture percentage; (5) test mass; (6) grain moisture percentage; (7) black dot stem nitrate tests (420 plots × 7 regions); (8) colonization with 1 plant per plot in a ziplock bag at 0% and 100% fertilizer at V4-V6 (1 plant x 14 treatments x 6 reps x 2 fertilization regimens = 168 plants); (9) transcriptomic with 1 plant per plot in a zipper bag at 0% and 100% fertilization at V4-V6 (1 plant x 14 treatments x 6 reps x 2 fertilization regimens = 168 plants); (10) determination of Normalized Difference Vegetation Index (NDVI) or Normalized Red Edge Difference (NDRE) using the Greenseeker tool at two time points (V4-V6 and VT) to evaluate each plot in all 7 regions (420 plots × 2 time points × 7 regions=5880 data points); (11) stem characteristics determined in all 7 regions between R2 and R5 by recording stem diameter 10 plants/plot at 15 cm height, recording first internode length above 15 cm mark, monitoring 10 plants (5 consecutive plants from the center of the two inner rows ) (420 plots × 10 plants × 7 regions=29400 data points).

[0370] Monitoring Scheme: Practitioners attended all trials during the V3-V4 stage to assess early seasonal response to treatments and during the reproductive growth stage to monitor maturation. A local employee continuously made exploratory tests.

[0371] Weather Information: Weather data was collected covering the period from planting to harvest, and consisted of daily minimum and maximum temperatures, soil temperature at planting, daily precipitation and irrigation (if applicable), and unusual weather events such as strong wind, rain, cold, heat.

[0372] Data presentation : Including the data above, data points were obtained in field trials, including soil textures; distance between rows; plot dimensions; irrigation; tillage; previous harvest; seeding rate; plant population; seasonal inputs of fertilizers, including source, rate, timing and location; the size of the harvested area, the method of harvesting, for example, by hand or machine, and the measuring instruments used (scales, yield monitor, etc.)

[0373] Results: Selected results from the above field trial are shown in Figure 32 and Figure 33 .

[0374] In figure 32 , it can be seen that the use of the microorganism of the invention (i.e., 6-403) resulted in a higher yield than the wild type strain (wild type) and a higher yield than the untreated control (UTC). The "-25 lbs N" treatment used 25 lbs N per acre less than standard farming practice in the region. The UTC treatment "100% N" is intended to refer to a region's standard agricultural practice in which 100% of the standard N use is applied by the farmer. The microorganism "6-403" was deposited under NCMA ID 201708004 and can be found in Table A. It is a mutant of Kosakonia sacchari (also referred to as CM037) and is a mutant offspring of wild-type strain CI006.

[0375] In Figure 33, the yield results obtained demonstrate that the microorganisms of the present invention act in different regions in a uniform manner. In addition, the yield results demonstrate that the microorganisms of the present invention function well both in an environment with insufficient nitrogen content (i.e., an environment with nitrogen restriction) and in an environment that has sufficient nitrogen content (i.e., an environment without nitrogen restriction). The microorganism "6-881" (also known as CM094, PBC6.94), and which is a mutant progeny of wild-type strain Kosakonia sacchari CI006, is deposited under NCMA ID 201708002 and can be found in Table A. The microorganism "137-1034", which is a mutant descendant of the wild-type strain Klebsiella variicola CI137, has been deposited under NCMA ID 201712001 and can be found in Table A. The microorganism "137-1036", which is a mutant descendant of the wild-type strain Klebsiella variicola CI137, has been deposited under NCMA ID 201712002 and can be found in Table A. The microorganism "6-404" (also known as CM38, PBC6.38), and which is a mutant progeny of the wild-type strain Kosakonia sacchari CI006, is deposited under NCMA ID 201708003 and can be found in Table A.

Example 12 Non-intergeneric Microorganism Genus Effective for Agricultural Systems

[0376] The microorganisms of the present invention were evaluated and compared with each other in terms of nitrogen production occurring per acre during the season. See figure 20 , figure 40 and figure 41

[0377] The inventors hypothesized that for a population of genetically engineered non-intergeneric microorganisms to be effective in a modern row crop agricultural system, the microbial population must produce at least one pound or more of nitrogen per acre per season.

[0378] Based on this, the inventors unexpectedly identified a functional genus of microorganisms that, among other things, are able to contribute to: increasing yields in non-legume crops; and/or reducing the farmer's dependence on the use of exogenous nitrogen; and/or the ability to produce at least one pound of nitrogen per acre per season, even in non-nitrogen-restricting environments, where the specified genus is defined by the product of colonizing capacity × mmol N produced per microorganism per hour (i.e., the line dividing figures 20 , 40 and 41 ).

[0379] Referring to Figures 20 , 40, and 41 , certain inventive microorganism usage data are combined to display a heat map of the number of pounds of nitrogen delivered per acre/season by inventive micro-organisms, which is recorded as a function of microorganisms per gram wet weight per mmol of nitrogen/microorganism /hour. Below the thin line across the larger image are microorganisms delivering less than one pound of nitrogen per acre/season, and above the line are microorganisms delivering more than one pound of nitrogen per acre/season.

[0380] Field Data and Heatmap Wild Type Colonization: The microorganisms used in the heatmap of Figure 20 were analyzed for N production in corn. For wild-type strains CI006 and CI019, maize root colonization data were obtained from a single field plot. For the remaining strains, colonization was assumed to be the same as the wild-type level in the field. N fixation activity was determined using an in vitro ARA assay with 5 mM glutamine. In the table below, the heat map in Figure 20 gives the exact value of mmol N produced by a microorganism per hour (mmol N/microorganism/hour), together with the exact values of CFU per gram wet weight (CFU/g wt.) for each microorganism, given on the heat map.

[0381] Heat map of field data: The data used in the heat map of figure 40 was obtained from strains of microorganisms evaluated for N production in corn in the field. Each point represents pounds N/acre produced by the microorganism using corn root colonization data from one field plot. N fixation activity was determined using an in vitro ARA assay with 5 mM N in the form of glutamine or ammonium phosphate. Table C below gives the exact mmol N produced by a microorganism per hour (mmol N/microorganism/hr) along with the exact CFU per gram wet weight (CFU/g wt) for each microorganism shown on the heat map of the figure . 40 .

[0382] Heatmap Based on Greenhouse and Laboratory Data: The data used in the heatmap of Figure 41 was obtained from strains of microorganisms assessed for N production in corn under laboratory and greenhouse conditions. Each dot represents pounds N/acre produced by one strain. White dots represent strains from which corn root colonization data were collected under greenhouse conditions. Black dots represent mutant strains for which corn root colonization levels are derived from average field corn root colonization levels of the wild-type parental strain. The shaded dots represent wild-type parental strains with their mean field levels of corn root colonization. In all cases, N fixation activity was determined by in vitro ARA assay with 5 mM N in the form of glutamine or ammonium phosphate. Table D below gives the exact mmol N produced by a microorganism per hour (mmol N/microorganism/hr) along with the exact CFU per gram wet weight (CFU/g wt) for each microorganism shown on the heat map of figure 41 .

Table C: figure 40 - Heat map for field data

Strain name Activity (mmol N/microorganism hour) Peak Colonization (CFU/g wt.) Produced N/acre/season Taxonomic designation CI006 3.88E-16 1.50E+07 0.24 Kosakonia sacchari 6-404 1.61E-13 3.50E+05 2.28 Kosakonia sacchari 6-848 1.80E-13 2.70E+05 1.97 Kosakonia sacchari 6-881 1.58E-13 5.00E+05 3.20 Kosakonia sacchari 6-412 4.80E-14 1.30E+06 2.53 Kosakonia sacchari 6-403 1.90E-13 1.30E+06 10.00 Kosakonia sacchari CI019 5.33E-17 2.40E+06 0.01 Rahnella aquatilis 19-806 6.65E-14 2.90E+06 7.80 Rahnella aquatilis 19-750 8.90E-14 2.60E+05 0.94 Rahnella aquatilis 19-804 1.72E-14 4.10E+05 0.29 Rahnella aquatilis CI137 3.24E-15 6.50E+06 0.85 Klebsiella variicola 137-1034 1.16E-14 6.30E+06 2.96 Klebsiella variicola 137-1036 3.47E-13 1.30E+07 182.56 Klebsiella variicola 137-1314 1.70E-13 1.99E+04 0.14 Klebsiella variicola 137-1329 1.65E-13 7.25E+04 0.48 Klebsiella variicola 63 3.60E-17 3.11E+05 0.00 Rahnella aquatilis 63-1146 1.90E-14 5.10E+05 0.39 Rahnella aquatilis 1021 1.77E-14 2.69E+07 19.25 Kosakonia pseudosacchari 728 5.56E-14 1445240.09 3.25 Klebsiella variicola

Table D: figure 41 - Heat map for greenhouse and laboratory data

Strain name Activity (mmol N/microorganism hour) Peak Colonization (CFU/g wt.) Produced N/acre/season Taxonomic designation CI006 3.88E-16 1.50E+07 0.24 Kosakonia sacchari 6-400 2.72E-13 1.79E+05 1.97 Kosakonia sacchari 6-397 1.14E-14 1.79E+05 0.08 Kosakonia sacchari CI137 3.24E-15 6.50E+06 0.85 Klebsiella variicola 137-1586 1.10E-13 1.82E+06 8.10 Klebsiella variicola 137-1382 4.81E-12 1.82E+06 354.60 Klebsiella variicola 1021 1.77E-14 2.69E+07 19.25 Kosakonia pseudosacchari 1021-1615 1.20E-13 2.69E+07 130.75 Kosakonia pseudosacchari 1021-1619 3.93E-14 2.69E+07 42.86 Kosakonia pseudosacchari 1021-1612 1.20E-13 2.69E+07 130.75 Kosakonia pseudosacchari 1021-1623 4.73E-17 2.69E+07 0.05 Kosakonia pseudosacchari 1293 5.44E-17 8.70E+08 1.92 Azospirillum lipoferum 1116 1.05E-14 1.37E+07 5.79 Enterobacter sp. 1113 8.05E-15 4.13E+07 13.45 Enterobacter sp. 910 1.19E-13 1.34E+06 6.46 Kluyvera intermedia 910-1246 2.16E-13 1.34E+06 11.69 Kluyvera intermedia 850 7.2301E-16 1.17E+06 0.03 Achromobacter spiritinus 852 5.96E-16 1.07E+06 0.03 Achromobacter marplatensis 853 6.42E-16 2.55E+06 0.07 Microbacterium murale

[0383] Conclusions: The data in figures 20 , 40 , 41 and in tables C and D illustrate a large number of representatives of the described genus (ie microorganisms to the right of the line in the figures). In addition, these numerous members come from a diverse array of taxonomic genera that can be found in Tables C and D above. In addition, the inventors have identified a variety of genetic properties that reflect the structure/function relationship found in many microorganisms. This genetic dependency can be found in a variety of tables of the invention, which list the genetic modifications carried out by the inventors, which include the introduction of at least one genetic variation in at least one gene or non-coding polynucleotide of the nitrogen fixation or assimilation genetic regulatory network.

[0384] Thus, the newly identified genus is supported by: (1) a robust dataset, (2) a large number of members, (3) members from different taxonomic genera, and (4) classes of genetic modifications that define a structure/function relationship in the underlying basis of the genetic architecture of representatives of the genus.

Example 13 Methods and Assays for Detecting Non-Intergeneric Genetically Engineered Microorganisms

[0385] In the present description, primers, probes and assays are indicated that are suitable for the detection of microorganisms used in the various examples above. The assays can detect sequences with non-natural "connection" of nucleotides in the resulting/mutant non-intergeneric microorganisms. These non-natural nucleotide compounds can be used as a type of diagnostic that is indicative of the presence of a particular genetic alteration in a microorganism.

[0386] The methods of the present invention can detect these non-natural nucleotide compounds using specialized quantitative PCR methods, including uniquely designed primers and probes. Probes can bind to sequences with non-natural connection of nucleotides. Those. you can use sequence-specific DNA-containing probes, consisting of oligonucleotides that are labeled with a fluorescent reporter, providing detection only after hybridization of the probe with its complementary sequence. Quantitative methods can ensure that only non-natural nucleotide bonding will be amplified by said primers, and thus can be detected by a non-specific dye or by using a specific hybridization probe. Another aspect of the method is the selection of primers such that the primers flank either end of the sequence with the compound so that if an amplification reaction occurs, said sequence with the compound is present.

[0387] Thus, genomic DNA can be isolated from samples and used to quantify the presence of microorganisms of the invention using qPCR. The primers used in the qPCR reaction may be primers designed with Primer Blast (https://www.ncbi.nlm.nih.gov/tools/primer-blast/) to amplify unique regions of the wild type genome or unique regions subjected to genetic engineering of non-intergeneric mutant strains. The qPCR reaction can be performed using the SYBR GreenER qPCR SuperMix Universal kit (Thermo Fisher P/N 11762100), using only forward and reverse amplification primers; alternatively, the Kapa Probe Force kit (Kapa Biosystems P/N KK4301) with amplification primers and TaqMan probe containing FAM dye at the 5' end, internal ZEN quencher, and minor groove binder and fluorescent quencher at 3' can be used. -end (Integrated DNA Technologies).

[0388] Certain primer, probe, and non-naturally coupled sequences that can be used in qPCR methods are listed in Table E below. Specifically, sequences with non-natural connection can be found in SEQ ID NO: 372-405.

Table E: Detection of microorganisms

Main CI Name connections above/below connection SEQID NO 100 b.p. above connection SEQID NO 100 b.p. below connection SEQID NO Connection sequence "/" indicates connection Described. connections Forward primer SEQ reverse primer SEQ Probe SEQ 1021 ds1131 up 304 TGGTGTCCGGGCGAACGTCGCCAGGTGGCACAAATTGTCAGAACTACGACACGACTAACCGACCGCAGGAGTGTGCGATGACCCTGAATATGATGATGGA 338 TTCTTGGTTCTCTGGAGCCGCTTTATCGGCATCCTGACTGAAGAATTTGCAGGCTTCTTCCCAACCTGGCTTGCACCCGTGCAGGTAGTTGTGATGAACAT 372 5'-TGGTGTCCGGGC
GAACGTCGCCAG
GTGGCACAAATT
GTCAGAACTACG
ACACGACTAACC
GACCGCAGGAG
TGTGCGATGACC
CTGAATATGATG
ATGGA/TTCTTGG
TTTCTCTGGAGCG
CTTTATCGGCAT
CCTGACTGAAGA
ATTTGCAGGCTT
CTTCCCAACCTG
GCTTGCACCCGT
GCAGGTAGTTGT
GATGAACAT-3' disrupted nifL/PinfC gene n.p. n.p. n.p. 1021 ds1131 down 305 CGGAAAACGAGTTCAAACGGCGCGTCCCAATCGTATTAATGGCGAGATTCGCGCCACGGAAGTTCGCTTAACAGGTCTGGAAGGCGAGCAGCTTGGTATT 339 GCGATAGAACTCACTTCACGCCCCGAAGGGGGAAGCTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGACCCAGCGAAC 373 5'-
CGGAAAACGAG
TTCAAACGGCGC
GTCCCAATCGTATTAATGGCGAGATTTCGCGCCACGGAAGTTCGCTTAACAGGTCTGGAAGGCGAGCAGCTTGGTATT/GCGATAGAACTCACTTCACGCCCCGAAGGGGGAAGCTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGACCCAGCGAAC
-3' PinfC/destroyed nifL gene n.p. n.p. n.p. 1021 ds1133 n.p. 306 CGCCAGAGAGTTGAAATCGAACATTTCCGTAATACCGCCATTACCCAGGAGCCGTTCTGGTTGCACAGCGGAAAACGTTAACGAAAGGATATTTCGCATG 340 TCCCTGTGCGCCGCGTCGCCGATGGTGGCCAGCCAACTGGCGCGCTACCCGATCCTGCTCGATGAACTGCTCGACCCGAACACGCTCTATCAACCGACGG 374 5'-CGCCAGAGAGTT
GAAATCGAACAT
TTCCGTAATACC
GCCATTACCAG
GAGCCGTTCTGG
TTGCACAGCGGA
AAACGTTAACGA
AAGGATATTTCG
CATG/TCCCTGTG
CGCCGCGTCGCC
GATGGTGGCCAG
CCAACTGGCGCG
CTACCCGATCCT
GCTCGATGAACT
GCTCGACCCGAA
CACGCTCTATCA
ACCGACGG-3' 5'-UTR and ATG/truncated glnE gene n.p. n.p. n.p. 1021 ds1145 up 307 CGGGCGAACGTCGCCAGGTGGCACAAATTGTCAGAACTACGACACGACTAACCGACCGCAGGAGTGTGCGATGACCCTGAATATGATGATGGATGCCAGC 341 CGTTCTGTAATAATAACCGGACAATTCGGACTGATTAAAAAAGCGCCCTCGCGGCCTTTTTTTATATTCTCGACTCCATTTAAAATAAAAAATCCAATC 375 5'-CGGGCGAACGTC
GCCAGGTGGCAC
AAATTGTCAGAA
CTACGACACGAC
TAACCGACCGCA
GGAGTGTGCGAT
GACCCTGAATAT
GATGATGGATGC
CAGC/CGTTCTGT
AATAATAACCGG
ACAATTCGGACT
GATTAAAAAAG
CGCCCTCGCGGC
GCTTTTTTTATAT
TCTCGACTCCAT
TTAAAATAAAAA
ATCCAATC-3' disrupted nifL/Prm1 gene n.p. n.p. n.p. 1021 ds1145 down 308 TCAACCTAAAAAAGTTTGTGTAATACTTGTAACGCTACATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAATCGTACTAAACTGGTACTGGGCGC 342 AACTCACTTCACGCCCCGAAGGGGGAAGCTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGACCCAGCGAACCGAGTCG 376 5'-TCAACCTAAAAA
AGTTTGTGTAAT
ACTTGTAACGCT
ACATGGAGATTA
ACTCAATCTAGA
GGGTATTAATAA
TGAATCGTACTA
AACTGGTACTGG
GCGC/AACTCAC
TTCACGCCCCGA
AGGGGGAAGCT
GCCTGACCCTAC
GATTCCCGCTAT
TTCATTCACTGA
CCGGAGGTTCAA
AATGACCCAGCG
AACCGAGTCG-3' Prm1/destroyed nifL gene n.p. n.p. n.p. 1021 ds1148 up 309 CGGGCGAACGTCGCCAGGTGGCACAAATTGTCAGAACTACGACACGACTAACCGACCGCAGGAGTGTGCGATGACCCTGAATATGATGATGGATGCCAGC 343 CGCGTCAGGTTGAACGTAAAAAAGTCGGTCTGCGCAAAGCACGTCGTCGTCCGCAGTTCTCCAAACGTTAATTGGTTTCTGCTTCGGCAGAACGATTGGC 377 5'-CGGGCGAACGTC
GCCAGGTGGCAC
AAATTGTCAGAA
CTACGACACGAC
TAACCGACCGCA
GGAGTGTGCGAT
GACCCTGAATAT
GATGATGGATGC
CAGC/CGCGTCA
GGTTGAACGTAA
AAAAGTCGGTCT
GCGCAAAGCAC
GTCGTCGTCGCGC
AGTTCTCCAAAC
GTTAATTGGTTT
CTGCTTCGGCAG
AACGATTGGC-3' disrupted nifL/Prm7 gene n.p. n.p. n.p. 1021 ds1148 down 310 AATTTTCTGCCCAAATGGCTGGGATTGTTCATTTTTTGTTTGCCTTACAACGAGAGTGACAGTACGCGCGGGTAGTTAACTCAACATCTGACCGGTCGAT 344 AACTCACTTCACGCCCCGAAGGGGGAAGCTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGACCCAGCGAACCGAGTCG 378 5'-AATTTTCTGCCC
AAATGGCTGGG
ATTGTTCATTTTT
TGTTTGCCTTAC
AACGAGAGTGA
CAGTACGCGCGG
GTAGTTAACTCA
ACATCTGACCGG
TCGAT/AACTCA
CTTCACGCCCCG
AAGGGGGAAGC
TGCCTGACCCTA
CGATTCCCGCTA
TTTCATTCACTG
ACCGGAGGTTCA
AAATGACCCAGC
GAACCGAGTCG-
3' Prm4/destroyed nifL gene n.p. n.p. n.p. CI006 ds126 n.p. 311 GTAACCAATAAAGGCCACCACGCCAGACCACACGATAGTGATGGCAACACTTTCCAGCTGCACCAGCACCTGATGGCCCATGGTCACACCTTCAGCGAAA 345 CCGATCCCCATCACTGTGTGTCTTGTATTACAGTGCCGCTTCGTCGGCTTCGCCGGTACGAATACGAATGACGCGTTGCAGCTCAGCAACGAAAATTTTG 379 5'-GTAACCAATAAA
GGCCACCACCGCC
AGACCACACGAT
AGTGATGGCAAC
ACTTTCCAGCTG
CACCAGCACCTG
ATGGCCCATGGT
CACACCTTCAGC
GAAA/CCGATCC
CCATCACTGTGT
GTCTTGTATTAC
AGTGCCGCTTCG
TCGGCTTTCGCCG
GTACGAATACGA
ATGACGCGTTGC
AGCTCAGCAACG
AAAATTTTG-3' 5'-UTR to ATG-4 b.p. amtB gene/damaged amtB gene n.p. n.p. n.p. CI019 ds172 down 312 TGGTATTGTCAGTCTGAATGAAGCTCTTGAAAAAGCTGAGGAAGCGGGCGTCGATTTAGTAGAAATCAGTCCGAATGCCGAGCCGCCAGTTTGTCGAATC 346 CCGTCTCTGAAGCTCTCGGTGAACATTGTTGCGAGGCAGGATGCGAGCTGGTTGTGTTTTGACATTACCGATAATGTGCCGCGTGAACGGGTGCGTTATG 380 5'-TGGTATTGTCAG
TCTGAATGAAGC
TCTTGAAAAAGC
TGAGGAAGCGG
GCGTCGATTTAG
TAGAAATCAGTC
CGAATGCCGAGC
CGCCAGTTTGTC
GAATC/CCGTCTC
TGAAGCTCTCGG
TGAACATTGTTG
CGAGGCAGGAT
GCGAGCTGGTTG
TGTTTTGACATT
ACCGATAATGTG
CCGCGTGAACGG
GTGCGTTATG-3' Prm1.2/destroyed nifL gene SEQ ID NO:406 CAAGAA
GTTCGC
CTCACA
GG SEQ ID NO:407 TGCC
TCGC
AACA
ATGT
TCAC n.p. CI019 ds172 up 313 ACCGATCCGCAGGCGCCGCATTTGTTATGCCAATCCGGCATTCTGCCGCCAGACGGGTTTTGCACTTGAGACACTTTTGGGCGAGAACCACCGTCTGCTGG 347 TGAACATCACTGATGCACAAGCTACCTATGTCGAAGAATTAACTAAAAAAACTGCAAGATGCAGGCATTCGCGTTAAAGCCGACTTGAGAAATGAGAAGAT 381 5'-ACCGATCCGCAG
GCGCGCATTTGT
TATGCCAATCCG
GCATTCTGCCGC
CAGACGGGTTTT
GCACTTGAGACA
CTTTTGGGCGAG
AACCACCGTCTG
CTGG/TGAACAT
CACTGATGCACA
AGCTACCTATGT
CGAAGAATTAAC
TAAAAAACTGCA
AGATGCAGGCAT
TCGCGTTAAAGC
CGACTTGAGAAA
TGAGAAGAT-3' disrupted nifL/Prm1.2 gene n.p. n.p. n.p. CI019 ds175 down 314 CGGGAACCGGTGTTATAATGCCGCGCCCTCATATTGTGGGGATTTCTTAATGACCTATCCTGGGTCCTAAAGTTGTAGTTGACATTAGCGGAGCACTAAC 348 CCGTCTCTGAAGCTCTCGGTGAACATTGTTGCGAGGCAGGATGCGAGCTGGTTGTGTTTTGACATTACCGATAATGTGCCGCGTGAACGGGTGCGTTATG 382 5'-CGGGAACCGGT
GTTATAATGCCG
CGCCCTCATATT
GTGGGGATTCTCT
TAATGACCTATC
CTGGGTCCTAA
GTTGTAGTTGAC
ATTAGCGGAGCA
CTAAC/CCGTCTC
TGAAGCTCTCGG
TGAACATTGTTG
CGAGGCAGGAT
GCGAGCTGGTTG
TGTTTTGACATT
ACCGATAATGTG
CCGCGTGAACGG
GTGCGTTATG-3' Prm3.1/destroyed nifL gene SEQ ID NO:408 CGCCCT
CATTT
GTGGGG
AT SEQ ID NO:409 GGCA
TAAC
GCAC
CCGT
TCA SEQ ID NO:410 /56-FA
M/T
A
AC
C
CG
T
C/Z
EN/
T
CT
G
AAG
CTC
TC
G
GT/
3IA
BkF
Q/ CI019 ds175 up 315 ACCGATCCGCAGGCGCCGCATTTGTTATGCCAATCCGGCATTCTGCCGCCAGACGGGTTTTGCACTTGAGACACTTTTGGGCGAGAACCACCGTCTGCTGG 349 TACAGTAGCCGCCTCTCAAAAATAGATAAACGGCTCATGTACGTGGGCCGTTTATTTTTTCTACCCATAATCGGGAACCGGTGTTATAATGCCGCGCCCTC 383 5'-ACCGATCCGCAG
GCGCGCATTTGT
TATGCCAATCCG
GCATTCTGCCGC
CAGACGGGTTTT
GCACTTGAGACA
CTTTTGGGCGAG
AACCACCGTCTG
CTGG/TACAGTA
GCGCCTCTCAAA
AATAGATAAAC
GGCTCATGTACG
TGGGCCGTTTAT
TTTTTCTACCCA
TAATCGGGAACC
GGTGTTATAATG
CCGCGCCCTC-3' disrupted nifL/Prm3.1 gene n.p. n.p. n.p. CI006 ds20 down 316 TCAACCTAAAAAAGTTTGTGTAATACTTGTAACGCTACATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAATCGTACTAAACTGGTACTGGGCGC 350 AACTCACTTCACACCCCCGAAGGGGGAAGTTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGACCCAGCGAACCGAGTCG 384 5'-TCAACCTAAAAA
AGTTTGTGTAAT
ACTTGTAACGCT
ACATGGAGATTA
ACTCAATCTAGA
GGGTATTAATAA
TGAATCGTACTA
AACTGGTACTGG
GCGC/AACTCAC
TTCACACCCCGA
AGGGGGAAGTT
GCCTGACCCTAC
GATTCCCGCTAT
TTCATTCACTGA
CCGGAGGTTCAA
AATGACCCAGCG
AACCGAGTCG-3' Prm1/destroyed nifL gene SEQ ID NO:411 TAAACT
GGTACT
GGGCGC
AACT SEQ ID NO:412 CAAA
TCGA
AGCG
CCAG
ACGG
TAT SEQ ID NO:413 /56-
FA
M/A
AG
TTG
CCT
/ZE
N/G
AC
CCT
AC
GA
TTC
CC/
3IA
BkF
Q/ CI006 ds20 up 317 GGGCGACAAACGGCCTGGTGGCACAAATTGTCAGAACTACGACACGACTAACTGACCGCAGGAGTGTGCGATGACCCTGAATATGATGATGGATGCCGGC 351 CGTCCTGTAATAATAACCGGACAATTCGGACTGATTAAAAAAGCGCCCTTGTGGCCGCTTTTTTTATATTCCCGCCTCCATTTAAAATAAAAAATCCAATC 385 5'-GGGCGACAAAAC
GGCCTGGTGGCA
CAAATTGTCAGA
ACTACGACACGA
CTAACTGACCGC
AGGAGTGTGCG
ATGACCCTGAAT
ATGATGATGGAT
GCCGGC/CGTCC
TGTAATAATAAC
CGGACAATTCGG
ACTGATTAAAAA
AGCGCCCTTGTG
GCGCTTTTTTTA
TATTCCCGCCTC
CATTTAAAATAA
AAAATCCAATC-
3' disrupted nifL/Prm1 gene n.p. n.p. n.p. CI006 ds24 up 318 GGGCGACAAACGGCCTGGTGGCACAAATTGTCAGAACTACGACACGACTAACTGACCGCAGGAGTGTGCGATGACCCTGAATATGATGATGGATGCCGGC 352 GGACATCATCGCGACAAACAATATTAATACCGGCAACCACACCGGCAATTTACGAGACTGCGCAGGCATCCTTTCTCCCGTCAATTTCTGTCAAATAAAG 386 5'-GGGCGACAAAAC
GGCCTGGTGGCA
CAAATTGTCAGA
ACTACGACACGA
CTAACTGACCGC
AGGAGTGTGCG
ATGACCCTGAAT
ATGATGATGGAT
GCCGGC/GGACA
TCATCGCGACAA
ACAATATTAATA
CCGGCAACCACA
CCGGCAATTTAC
GAGACTGCGCA
GGCATCCTTTCT
CCCGTCAATTTC
TGTCAAATAAAG
-3' disrupted nifL/Prm5 gene SEQ ID NO:414 GGTGCA
CTCTTT
GCATGG
TT SEQ ID NO:415 GCGC
AGTC
TCGT
AAAT
TGCC SEQ ID NO:416 /56-
FA
M/C
A
GG
A
GT
G
T/Z
EN/G
CG
A
TG
A
CC
C
TG
A
AT/
3IA
BkF
Q CI006 ds24 down 319 TAAGAATTATCTGGATGAATGTGCCATTAAATGCGCAGCATAATGGTGCGTTGTGCGGGAAAACTGCTTTTTTTTGAAAGGGTTGGTCAGTAGCGGAAAC 353 AACTCACTTCACACCCCCGAAGGGGGAAGTTGCCTGACCCTACGATTCCCGCTATTTCATTCACTGACCGGAGGTTCAAAATGACCCAGCGAACCGAGTCG 387 5'-TAAGAATTATCT
GGATGAATGTGC
CATTAAATGCGC
AGCATAATGGTG
CGTTGTGCGGGA
AAACTGCTTTTTT
TTTGAAAGGGTT
GGTCAGTAGCGG
AAAC/AACTCAC
TTCACACCCCGA
AGGGGGAAGTT
GCCTGACCCTAC
GATTCCCGCTAT
TTCATTCACTGA
CCGGAGGTTCAA
AATGACCCAGCG
AACCGAGTCG-3' Prm5/destroyed nifL gene n.p. n.p. n.p. CI006 ds30 n.p. 320 CGCCAGAGAGTCGAAATCGAACATTTCCGTAATACCGCGATTACCCAGGAGCCGTTCTGGTTGCACAGCGGAAAACGTTAACGAAAGGATATTTCGCATG 354 TTTAACGATCTGATTGGCGATGATGAAACGGATTCGCCGGAAGATGCGCTTTCTGAGAGCTGGCGCGAATTGTGGCAGGATGCGTTGCAGGAGGAGGATT 388 5'-CGCCAGAGAGTC
GAAATCGAACAT
TTCCGTAATACC
GCGATTACCCAG
GAGCCGTTCTGG
TTGCACAGCGGA
AAACGTTAACGA
AAGGATATTTCG
CATG/TTTAACG
ATCTGATTGGCG
ATGATGAAACG
GATTCGCCGGAA
GATGCGCTTTCT
GAGAGCTGGCG
CGAATTGTGGCA
GGATGCGTTGCA
GGAGGAGGATT-
3' 5'-UTR and ATG/truncated glnE gene n.p. n.p. n.p. CI006 ds31 n.p. 321 CGCCAGAGAGTCGAAATCGAACATTTCCGTAATACCGCGATTACCCAGGAGCCGTTCTGGTTGCACAGCGGAAAACGTTAACGAAAGGATATTTCGCATG 355 GCACTGAAAACCTCATTTCCCTGTGTGCCGCGTCGCCGATGGTTGCCAGTCAGCTGGCGCGCTACCCGATCCTGCTTGATGAATTGCTCGACCCGAATA 389 5'-CGCCAGAGAGTC
GAAATCGAACAT
TTCCGTAATACC
GCGATTACCCAG
GAGCCGTTCTGG
TTGCACAGCGGA
AAACGTTAACGA
AAGGATATTTCG
CATG/GCACTGA
AACACCTCATTT
CCCTGTGTGCCG
CGTCCGCCGATGG
TTGCCAGTCAGC
TGGCGCGCTACC
CGATCCTGCTTG
ATGAATTGCTCG
ACCCGAATA-3' 5'-UTR and ATG/truncated glnE gene n.p. n.p. n.p. CI019 ds34 n.p. 322 GATGATGGATGCTTTCTGGTTAAACGGGCAACCTCGTTAACTGACTGACTAGCCTGGGCAAACTGCCCGGGCTTTTTTTTGCAAGGAATCTGATTTCATG 356 GCGCTCAAACAGTTAATCCGTCTGTGTGCCGCCTCGCCGATGGTCGCGACACAACTTGCACGTCATCCTTTATTGCTCGATGAACTGCTCGACCCGCGCA 390 5'-GATGATGGATGC
TTTCTGGTTAAA
CGGGCAACCTCG
TTAACTGACTGA
CTAGCCTGGGCA
AACTGCCCGGGC
TTTTTTTTGCAA
GGAATCTGATTTT
CATG/GCCGCTCA
AACAGTTAATCC
GCTTGTGTGCCG
CCTCGCCGATGG
TCGCGACACAAC
TTGCACGTCATC
CTTTATTGCTCG
ATGAACTGCTCG
ACCCCGCGCA-3' 5'-UTR and ATG/truncated glnE gene n.p. n.p. n.p. CI019 ds70 up 323 ACCGATCCGCAGGCGCCGCATTTGTTATGCCAATCCGGCATTCTGCCGCCAGACGGGTTTTGCACTTGAGACACTTTTGGGCGAGAACCACCGTCTGCTGG 357 AGTCTGAACTCATCCTGCGGCAGTCGGTGAACGTATTTTTTGACCAAAGAGTGATCTACATCACGGAATTTTGTGGTTGTTGCTGCTTAAAAGGGCAAAT 391 5'-ACCGATCCGCAG
GCGCGCATTTGT
TATGCCAATCCG
GCATTCTGCCGC
CAGACGGGTTTT
GCACTTGAGACA
CTTTTGGGCGAG
AACCACCGTCTG
CTGG/AGTCTGA
ACTCATCCTGCG
GCAGTCGGTGAG
ACGTATTTTGA
CCAAAGAGTGAT
CTACATCACGGA
ATTTTGTGGTTG
TTGCTGCTTAAA
AGGGCAAAT-3' disrupted nifL/Prm4 gene n.p. n.p. n.p. CI019 ds70 down 324 CATCGGACACCACCAGCTTACAAATTGCCTGATTGCGGCCCCGATGGCCGGTATCACTGACCGACCATTTCGTGCCTTATGTCATGCGATGGGGGCTGGG 358 CCGTCTCTGAAGCTCTCGGTGAACATTGTTGCGAGGCAGGATGCGAGCTGGTTGTGTTTTGACATTACCGATAATGTGCCGCGTGAACGGGTGCGTTATG 392 5'-CATCGGACACCA
CCAGCTTACAAA
TTGCCTGATTGC
GGCCCCGATGGC
CGGTATCACTGA
CCGACCATTTCG
TGCCTTTATGTCA
TGCGATGGGGGC
TGGG/CCGTCTCT
GAAGCTCTCGGT
GAACATTGTTGC
GAGGCAGGATG
CGAGCTGGTTGT
GTTTGACATTA
CCGATAATGTGC
CGCGTGAACGG
GTGCGTTATG-3' Prm4/destroyed nifL gene n.p. n.p. n.p. 137 ds799 down 325 TCTCAACAACTGGAGGAATAAGGTATTAAAGGCGGAAAACGAGTTCAAACGGCACGTCCGAATCGTATCAATGGCGAGATTCGCGCCCTGGAAGTTCGC 359 GCCATTGAGCTGGCTTCCCGACCGCAGGGCGGCACCTGCCTGACCCTGCGTTTCCCGCTGTTTAACACCCTGACCGGAGGTGAAGCATGATCCCTGAATC 393 5'-TCTTCAACAACT
GGAGGAATAAG
GTATTAAAGGCG
GAAAACGATTTC
AAACGGCACGTC
CGAATCGTATCA
ATGGCGAGATTC
GCGCCCTGGAAG
TTCGC/GCCATTG
AGCTGGCTTCCC
GACCGCAGGGC
GGCACCTGCCTG
ACCCTGCGTTTC
CCGCTGTTTAAC
ACCCTGACCGGA
GGTGAAGCATG
ATCCCTGAATC-
3' PinfC/destroyed nifL gene SEQ ID NO:417 CTCGGC
AGCATG
GACGTA
A SEQ ID NO:418 AGG
GTGT
TAAA
CAGC
GGG
AAA SEQ ID NO:419 /56-
FA
M/A
A
CG
G
CA
C
G/Z
EN/
T
CC
G
AA
T
CG
T
AT
C
AA/
3IA
BkF
Q/ 137 ds799 up 326 TCCGGGTTCGGCTTACCCCGCCGCGTTTTGCGCACGGTGTCGGACAATTTGTCATAACTGCGACACAGGAGTTTGCGATGACCCTGAATATGATGCTCGA 360 AGCGTCAGGTACCGGTCATGATTCACCGTGCGATTCTCGGTTCCCTGGAGCGCTTCATTGGCATCCTGACCGAAGAGTTCGCTGGCTTCTTCCCAACCTG 394 5'-TCCGGGTTCGGC
TTACCCCGCCGC
GTTTTGCGCACG
GTGTCGGACAAT
TTGTCATAACTG
CGACACAGGAG
TTTGCGATGACC
CTGAATATGATG
CTCGA/AGCGTC
AGGTACCGGTCA
TGATTCACCGTG
CGATTCTCGGTT
CCTGGAGCGCT
TCATTGGCATCC
TGACCGAAGAGT
TCGCTGGCTTCT
TCCCAACCTG-3' disrupted nifL/PinfC gene n.p. n.p. n.p. 137 ds809 n.p. 327 ATCGCAGCGTCTTGAATATTTCCGTCGCCAGGCGCTGGCTGCCGAGCCGTTCTGGCTGCATAGTGGAAAACGATAATTTCAGGCCAGGGAGCCCTTATG 361 GCGCTGAAGCACCTGATCACGCTCTGCGCGGCGTCGCCGATGGTCGCCAGCCAGCTGGCGCGCACCCGCTGCTGCTGGATGAGCTGCTGGATCCCAACA 395 5'-ATCGCAGCGTCT
TTGAATATTTCC
GTCGCCAGGCGC
TGGCTGCCGAGC
CGTTCTGGCTGC
ATAGTGGAAAA
CGATAATTTCAG
GCCAGGGAGCC
CTTATG/GCGCTG
AAGCACCTGATC
ACGCTCTGCGCG
GCGTCGCCGATG
GTCGCCAGCCAG
CTGGCGCCGCCAC
CCGCTGCTGCTG
GATGAGCTGCTG
GATCCCAACA-3' 5'-UTR and ATG/truncated glnE gene SEQ ID NO:420GAGCCG
TTCTGG
CTGCAT
AG SEQ ID NO:421 GCCG
TCGG
CTGA
TAGA
GG SEQ ID NO:422 /56-
FA
M/T
TAT
GG
CG
C/Z
EN/
TG
AA
GC
AC
CT
GA
TC
A/3I
ABk
FQ/ 137 ds843 up 328 TCCGGGTTCGGCTTACCCCGCCGCGTTTTGCGCACGGTGTCGGACAATTTGTCATAACTGCGACACAGGAGTTTGCGATGACCCTGAATATGATGCTCGA 362 GCCCGCTGACCGACCAGAACTTCCACCTTGGACTCGGCTATACCCTTGGCGTGACGGCGCGCGATAACTGGGACTACATCCCCCATTCCGGTGATCTTACC 396 5'-TCCGGGTTCGGC
TTACCCCGCCGC
GTTTTGCGCACG
GTGTCGGACAAT
TTGTCATAACTG
CGACACAGGAG
TTTGCGATGACC
CTGAATATGATG
CTCGA/GCCCGC
TGACCGACCAGA
ACTTCCACCTTG
GACTCGGCTATA
CCCTTGGCGTGA
CGGGCGCCGATA
ACTGGGACTACA
TCCCCATTCCGG
TGATCTTACC-3' disrupted nifL/Prm1.2 gene n.p. n.p. n.p. 137 ds843 down 329 TCACTTTTTAGCAAAGTTGCACTGGACAAAAGGTACCACAATTGGTGTACTGATACTCGACACAGCATTAGTGTCGATTTTCATATAAAGGTAATTTTG 363 GCCATTGAGCTGGCTTCCCGACCGCAGGGCGGCACCTGCCTGACCCTGCGTTTCCCGCTGTTTAACACCCTGACCGGAGGTGAAGCATGATCCCTGAATC 397 5'-TCACTTTTTAGC
AAAGTTGCACTG
GACAAAAGGTA
CCACAATTGGTG
TACTGATACTCG
ACACAGCATTAG
TGTCGATTTTTC
ATATAAAGGTAA
TTTTG/GCCATTG
AGCTGGCTTCCC
GACCGCAGGGC
GGCACCTGCCTG
ACCCTGCGTTTC
CCGCTGTTTAAC
ACCCTGACCGGA
GGTGAAGCATG
ATCCCTGAATC-
3' Prm1.2/destroyed nifL gene n.p. n.p. n.p. 137 ds853 up 330 TCCGGGTTCGGCTTACCCCGCCGCGTTTTGCGCACGGTGTCGGACAATTTGTCATAACTGCGACACAGGAGTTTGCGATGACCCTGAATATGATGCTCGA 364 GCTAAAGTTCTCGGCTAATCGCTGATAACATTTGACGCAATGCGCAATAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCATACCAGAACCTGGC 398 5'-TCCGGGTTCGGC
TTACCCCGCCGC
GTTTTGCGCACG
GTGTCGGACAAT
TTGTCATAACTG
CGACACAGGAG
TTTGCGATGACC
CTGAATATGATG
CTCGA/GCTAAA
GTTCTCGGCTAAA
TCGCTGATAACA
TTTGACGCAATG
CGCAATAAAAG
GGCATCATTTGA
TGCCCTTTTTGC
ACGCTTTCATAC
CAGAACCTGGC-
3' disrupted nifL/Prm6.2 gene n.p. n.p. n.p. 137 ds853 down 331 GTTCTCCTTTGCAATAGCAGGGAAGAGGCGCCAGAACCGCCAGCGTTGAAGCAGTTTGAACGCGTTCAGTGTATAATCCGAAACTTAATTTCGGTTTGGA 365 GCCATTGAGCTGGCTTCCCGACCGCAGGGCGGCACCTGCCTGACCCTGCGTTTCCCGCTGTTTAACACCCTGACCGGAGGTGAAGCATGATCCCTGAATC 399 5'-GTTTCTCCTTTGC
AATAGCAGGGA
AGAGGCCGCCAG
AACCGCCAGCGT
TGAAGCAGTTTG
AACGCGTTCAGT
GTATAATCCGAA
ACTTAATTTCGG
TTTGGA/GCCATT
GAGCTGGCTTCC
CGACCGCAGGG
CGGCACCTGCCT
GACCCTGCGTTT
CCCGCTGTTTAA
CACCCTGACCGG
AGGTGAAGCAT
GATCCCTGAATC
-3' Prm6.2/destroyed nifL gene n.p. n.p. n.p. 137 ds857 up 332 TCCGGGTTCGGCTTACCCCGCCGCGTTTTGCGCACGGTGTCGGACAATTTGTCATAACTGCGACACAGGAGTTTGCGATGACCCTGAATATGATGCTCGA 366 CGCCGTCTCGCAGTACCATTGCAACCGACTTTACAGCAAGAAGTGATTCTGGCACGCATGGAACAAATTCTTGCCAGTCGGGCTTTATCCGATGACGAA 400 5'-TCCGGGTTCGGC
TTACCCCGCCGC
GTTTTGCGCACG
GTGTCGGACAAT
TTGTCATAACTG
CGACACAGGAG
TTTGCGATGACC
CTGAATATGATG
CTCGA/CGCCGT
CCTCGCAGTACC
ATTGCAACCGAC
TTTACAGCAAGA
AGTGATTCTGGC
ACGCATGGAAC
AAATTCTTGCCA
GTCGGGCTTTTAT
CCGATGACGAA-
3' disrupted nifL/Prm8.2 gene n.p. n.p. n.p. 137 ds857 down 333 GATATGCCTGAAGTATTCAATTACTTAGGCATTTACTTAACGCAGGCAGGCAATTTTGATGCTGCCTATGAAGCGTTTGATTCTGTACTTGAGCTTGATC 367 GCCATTGAGCTGGCTTCCCGACCGCAGGGCGGCACCTGCCTGACCCTGCGTTTCCCGCTGTTTAACACCCTGACCGGAGGTGAAGCATGATCCCTGAATC 401 5'-GATATGCCTGAA
GTATTCAATTAC
TTAGGCATTTAC
TTAACGCAGGCA
GGCAATTTTGAT
GCTGCCTATGAA
GCGTTTTGATTCT
GTACTTGAGCT
GATC/GCCATTG
AGCTGGCTTCCC
GACCGCAGGGC
GGCACCTGCCTG
ACCCTGCGTTTC
CCGCTGTTTAAC
ACCCTGACCGGA
GGTGAAGCATG
ATCCCTGAATC-
3' Prm8.2/destroyed nifL gene n.p. n.p. n.p. 63 ds908 down 334 TGGTATTGTCAGTCTGAATGAAGCTCTTGAAAAAGCTGAGGAAGCGGGCGTCGATTTAGTAGAAATCAGTCCGAATGCCGAGCCGCCAGTTTGTCGAATC 368 TCTTTAGATCTCTCGGTCCGCCCTGATGGCGGCACCTTGCTGACGTTACGCCTGCCGGTACAGCAGGTTATCACCGGAGGCTTAAAATGACCCAGTTACC 402 5'-TGGTATTGTCAG
TCTGAATGAAGC
TCTTGAAAAAGC
TGAGGAAGCGG
GCGTCGATTTAG
TAGAAATCAGTC
CGAATGCCGAGC
CGCCAGTTTGTC
GAATC/TCTTTAG
ATCTCTCGGTCC
GCCTGATGGCG
GCACCTTGCTGA
CGTTACGCCTGC
CGGTACAGCAG
GTTATCACCGGA
GGCTTAAAATGA
CCCAGTTAC-3' PinfC/destroyed nifL gene SEQ ID NO:423 GGAAAAA
CGAGTT
CAACCG
GC SEQ ID NO:424 GGGC
GGAC
CGAG
AGAT
CTAA n.p. 63 ds908 up 335 TGCAAATTGCACGGTTATTCCGGGTGAGTATATGTGTGATTTGGGTTCCGGCATTGCGCAATAAAGGGGAGAAAGACATGAGCATCACGGCGTTATCAGC 369 TGAATATCACTGACTCACAAGCTACCTATGTCGAAGAATTAACTAAAAAAACTGCAAGATGCAGGCATTCGCGTTAAAGCCGACTTGAGAAATGAGAAGAT 403 5'-TGCAAATTGCAC
GGTTATTCCGGG
TGAGTATATGTG
TGATTTGGGTTC
CGGCATTGCGCA
ATAAAGGGGAG
AAAGACATGAG
CATCACGGCGTT
ATCAGC/TGAAT
ATCACTGACTCA
CAAGCTACCTAT
GTCGAAGAATTA
ACTAAAAAACTG
CAAGATGCAGG
CATTCGCGTTAA
AGCCGACTTGAG
AAATGAGAAGAT-3' disrupted nifL/PinfC gene n.p. n.p. n.p. 910 ds960 up 336 TCAGGGCTGCGGATGTCGGGCGTTTCACAACACAAAATGTTGTAAATGCGACACAGCCGGGCCTGAAACCAGGAGCGTGTGATGACCTTTAATATGATGC 370 CTGGGGTCACTGGAGCCGCTTTATCGGCATCCTGACCGAAGAATTTGCCGGTTTTTCCCGACCTGGCTGGCCCCTGTTCAGGTTGTGGTGATGAATATCA 404 5'-TCAGGGCTGCGG
ATGTCGGGCGTT
TCACAACACAAA
ATGTTGTAAATG
CGACACAGCCG
GGCCTGAAACCA
GGAGCGTGTGAT
GACCTTTAATAT
GATGC/CTGGGG
TCACTGGAGCGC
TTTATCGGCATC
CTGACCGAAGA
ATTTGCCGGTTT
CTTCCCGACCTG
GCTGGCCCCTGT
TCAGGTTGTGGT
GATGAATATCA-
3' disrupted nifL/PinfC gene n.p. n.p. n.p. 910 ds960 down 337 CGGAAAACGAGTTCAAACGGCACGTCCGAATCGTATCAATGGCGAGATTCGCGCCCAGGAAGTTCGCTTAACTGGTCTGGAAGGTGAGCAGCTGGGTATT 371 GCAATAGAACTAACTACCCGCCCTGAAGGCGGTACCTGCCTGACCCTGCGATTCCCGTTATTTCATTCACTGACCGGAGGCCCACGATGACCCAGCGACC 405 5'-CGGAAAACGAG
TTCAAACGGCAC
GTCCGAATCGTA
TCAATGGCGAGA
TTCGCGCCCAGG
AAGTTCGCTTAA
CTGGTCTGGAAG
GTGAGCAGCTGG
GTATT/GCAATA
GAACTAACTACC
CGCCCTGAAGGC
GGTACCTGCCTG
ACCCTGCGATTC
CCGTTATTTCAT
TCACTGACCGGA
GGCCCACGATGA
CCCAGCGACC-3' PinfC/destroyed nifL gene n.p. n.p. n.p.

Table F: Genetically engineered non-intergeneric microorganisms

Strain name Genotype SEQID NO CI006 16S rDNA - contig 5 62 CI006 16S rDNA - contig 8 63 CI019 16S rDNA 64 CI006 nifH 65 CI006 nifD 66 CI006 nifK 67 CI006 nifL 68 CI006 nifA 69 CI019 nifH 70 CI019 nifD 71 CI019 nifK 72 CI019 nifL 73 CI019 nifA 74 CI006 Prm5 with flanking regions 500 b.p. 75 CI006 nifLA operon - upstream intergenic region and CDS nifL and nifA 76 CI006 nifL (amino acid) 77 CI006 nifA (amino acid) 78 CI006 glnE 79 CI006 glnE_KO1 80 CI006 glnE (amino acid) 81 CI006 glnE_KO1 (amino acid) 82 CI006 ATase domain GlnE (amino acid) 83 CM029 Prm5 embedded in nifL area 84

Table G: Genetically engineered non-intergeneric microorganisms

Strain Strain ID SEQID NO Genotype Description Associated new connection if applicable CI63; CI063 63 SEQ ID NO 85 16S n.p. n.p. CI63; CI063 63 SEQ ID NO 86 nifH n.p. n.p. CI63; CI063 63 SEQ ID NO 87 nifD1 1 of 2 unique genes annotated in genome 63 as nifD n.p. CI63; CI063 63 SEQ ID NO 88 nifD2 2 of 2 unique genes annotated in genome 63 as nifD n.p. CI63; CI063 63 SEQ ID NO 89 nifK1 1 of 2 unique genes annotated in genome 63 as nifK n.p. CI63; CI063 63 SEQ ID NO 90 nifK2 2 of 2 unique genes annotated in genome 63 as nifK n.p. CI63; CI063 63 SEQ ID NO 91 nifL n.p. n.p. CI63; CI063 63 SEQ ID NO 92 nifA n.p. n.p. CI63; CI063 63 SEQ ID NO 93 glnE n.p. n.p. CI63; CI063 63 SEQ ID NO 94 amtB n.p. n.p. CI63; CI063 63 SEQ ID NO 95 PinfC 500 b.p. immediately upstream of the ATG start codon of the infC gene n.p. CI137 137 SEQ ID NO 96 16S n.p. n.p. CI137 137 SEQ ID NO 97 nifH1 1 of 2 unique genes annotated in genome 137 as nifH n.p. CI137 137 SEQ ID NO 98 nifH2 2 of 2 unique genes annotated in genome 137 as nifH n.p. CI137 137 SEQ ID NO 99 nifD1 1 of 2 unique genes annotated in genome 137 as nifD n.p. CI137 137 SEQID NO 100 nifD2 2 of 2 unique genes annotated in genome 137 as nifD n.p. CI137 137 SEQ ID NO 101 nifK1 1 of 2 unique genes annotated in genome 137 as nifK n.p. CI137 137 SEQ ID NO 102 nifK2 2 of 2 unique genes annotated in genome 137 as nifK n.p. CI137 137 SEQ ID NO 103 nifL n.p. n.p. CI137 137 SEQ ID NO 104 nifA n.p. n.p. CI137 137 SEQ ID NO 105 glnE n.p. n.p. CI137 137 SEQ ID NO 106 PinfC 500 b.p. immediately upstream of the start codon TTG of infC n.p. CI137 137 SEQ ID NO 107 amtB n.p. n.p. CI137 137 SEQ ID NO 108 Prm8.2 an internal promoter located in the nlpI gene; 299 b.p., starting from 81 b.p. after A ATG of the nlpI gene n.p. CI137 137 SEQ ID NO 109 Prm6.2 300 b.p. upstream of the secE gene, starting from 57 bp above A ATG secE n.p. CI137 137 SEQ ID NO 110 Prm1.2 400 bp immediately upstream of the ATG of the cspE gene n.p. No 728 SEQ ID NO 111 16S n.p. n.p. No 728 SEQ ID NO 112 nifH n.p. n.p. No 728 SEQ ID NO 113 nifD1 1 of 2 unique genes annotated in genome 728 as nifD n.p. No 728 SEQ ID NO 114 nifD2 2 of 2 unique genes annotated in the 728 genome as nifD n.p. No 728 SEQ ID NO 115 nifK1 1 of 2 unique genes annotated in genome 728 as nifK n.p. No 728 SEQ ID NO 116 nifK2 2 of 2 unique genes annotated in the 728 genome as nifK n.p. No 728 SEQ ID NO 117 nifL n.p. n.p. No 728 SEQ ID NO 118 nifA n.p. n.p. No 728 SEQ ID NO 119 glnE n.p. n.p. No 728 SEQ ID NO 120 amtB n.p. n.p. No 850 SEQ ID NO 121 16S n.p. n.p. No 852 SEQ ID NO 122 16S n.p. n.p. No 853 SEQ ID NO 123 16S n.p. n.p. No 910 SEQ ID NO 124 16S n.p. n.p. No 910 SEQ ID NO 125 nifH n.p. n.p. No 910 SEQ ID NO 126 CDS of iron-molybdenum dinitrogenase cofactor n.p. n.p. No 910 SEQ ID NO 127 nifD1 n.p. n.p. No 910 SEQ ID NO 128 nifD2 n.p. n.p. No 910 SEQ ID NO 129 nifK1 n.p. n.p. No 910 SEQ ID NO 130 nifK2 n.p. n.p. No 910 SEQ ID NO 131 nifL n.p. n.p. No 910 SEQ ID NO 132 nifA n.p. n.p. No 910 SEQ ID NO 133 glnE n.p. n.p. No 910 SEQ ID NO 134 amtB n.p. n.p. No 910 SEQ ID NO 135 PinfC 498 b.p. immediately upstream of the infC gene ATG n.p. No 1021 SEQ ID NO 136 16S n.p. n.p. No 1021 SEQ ID NO 137 nifH n.p. n.p. No 1021 SEQ ID NO 138 nifD1 1 of 2 unique genes annotated in the 910 genome as nifD n.p. No 1021 SEQ ID NO 139 nifD2 2 of 2 unique genes annotated in the 910 genome as nifD n.p. No 1021 SEQ ID NO 140 nifK1 1 of 2 unique genes annotated in the 910 genome as nifK n.p. No 1021 SEQ ID NO 141 nifK2 2 of 2 unique genes annotated in the 910 genome as nifK n.p. No 1021 SEQ ID NO 142 nifL n.p. n.p. No 1021 SEQ ID NO 143 nifA n.p. n.p. No 1021 SEQ ID NO 144 glnE n.p. n.p. No 1021 SEQ ID NO 145 amtB n.p. n.p. No 1021 SEQ ID NO 146 PinfC 500 b.p. immediately upstream of the ATG start codon of the infC gene n.p. No 1021 SEQ ID NO 147 Prm1 348 b.p. includes 319 b.p. immediately upstream of the ATG start codon of the lpp gene and the first 29 b.p. lpp gene n.p. No 1021 SEQ ID NO 148 Prm7 339 b.p. upstream of the sspA gene, ends at 46 bp. upstream of the ATG of the sspA gene n.p. No 1113 SEQ ID NO 149 16S n.p. n.p. No 1113 SEQ ID NO 150 nifH n.p. n.p. No 1113 SEQ ID NO 151 nifD1 1 of 2 unique genes annotated in the 1113 genome as nifD n.p. No 1113 SEQ ID NO 152 nifD2 2 of 2 unique genes annotated in the 1113 genome as nifD n.p. No 1113 SEQ ID NO 153 nifK n.p. n.p. No 1113 SEQ ID NO 154 nifL n.p. n.p. No 1113 SEQ ID NO 155 partial nifA gene due to a missing sequence in the assembly, the authors can only identify a partial gene from the 1113 genome n.p. No 1113 SEQ ID NO 156 glnE n.p. n.p. No 1116 SEQ ID NO 157 16S n.p. No 1116 SEQ ID NO 158 nifH n.p. No 1116 SEQ ID NO 159 nifD1 1 of 2 unique genes annotated in the 1116 genome as nifD n.p. No 1116 SEQ ID NO 160 nifD2 2 of 2 unique genes annotated in the 1116 genome as nifD n.p. No 1116 SEQ ID NO 161 nifK1 1 of 2 unique genes annotated in the 1116 genome as nifK n.p. No 1116 SEQ ID NO 162 nifK2 2 of 2 unique genes annotated in the 1116 genome as nifK n.p. No 1116 SEQ ID NO 163 nifL n.p. n.p. No 1116 SEQ ID NO 164 nifA n.p. n.p. No 1116 SEQ ID NO 165 glnE n.p. n.p. No 1116 SEQ ID NO 166 amtB n.p. n.p. No 1293 SEQ ID NO 167 16S n.p. n.p. No 1293 SEQ ID NO 168 nifH n.p. n.p. No 1293 SEQ ID NO 169 nifD1 1 of 2 unique genes annotated in the 1293 genome as nifD n.p. No 1293 SEQ ID NO 170 nifD2 2 of 2 unique genes annotated in the 1293 genome as nifD n.p. No 1293 SEQ ID NO 171 nifK 1 of 2 unique genes annotated in the 1293 genome as nifK n.p. No 1293 SEQ ID NO 172 nifK1 2 of 2 unique genes annotated in the 1293 genome as nifK n.p. No 1293 SEQ ID NO 173 nifA n.p. n.p. No 1293 SEQ ID NO 174 glnE n.p. n.p. No 1293 SEQ ID NO 175 amtB1 1 of 2 unique genes annotated in the 1293 genome as amtB n.p. No 1293 SEQ ID NO 176 amtB2 2 of 2 unique genes annotated in the 1293 genome as amtB n.p. No 1021-1612 SEQ ID NO 177 ΔnifL::PinfC starting from 24 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the PinfC 1021 promoter sequence ds1131 No 1021-1612 SEQ ID NO 178 ΔnifL::PinfC with flanking 500 b.p. starting from 24 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the PinfC 1021 promoter sequence; included 500 bp flanking the nifL gene above and below ds1131 No 1021-1612 SEQ ID NO 179 glnEΔAR-2 glnE gene from 1673 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain ds1133 No 1021-1612 SEQ ID NO 180 glnEΔAR-2 with flanking 500 b.p. glnE gene from 1673 bp immediately downstream of the ATG start codon are deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain; included 500 bp flanking the glnE gene above and below ds1133 No 1021-1615 SEQ ID NO 181 ΔnifL::Prm1 starting from 24 b.p. after A start codon ATG, 1375 bp nifL are deleted and replaced with the Prm1 promoter sequence 1021 ds1145 No 1021-1615 SEQ ID NO 182 ΔnifL::Prm1 with flanking 500 b.p. starting from 24 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced by the rm1 1021 promoter sequence; included 500 bp flanking the nifL gene above and below ds1145 No 1021-1615 SEQ ID NO 183 glnEΔAR-2 glnE gene from 1673 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain ds1133 No 1021-1615 SEQ ID NO 184 glnEΔAR-2 with flanking 500 b.p. glnE gene from 1673 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain; included 500 bp flanking the glnE gene above and below ds1133 No 1021-1619 SEQ ID NO 185 ΔnifL::Prm1 starting from 24 b.p. after A start codon ATG, 1375 bp nifL are deleted and replaced with the Prm1 promoter sequence 1021 ds1145 No 1021-1619 SEQ ID NO 186 ΔnifL::Prm1 with flanking 500 b.p. starting from 24 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced by the rm1 1021 promoter sequence; included 500 bp flanking the nifL gene above and below ds1145 No 1021-1623 SEQ ID NO 187 glnEΔAR-2 glnE gene from 1673 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain ds1133 No 1021-1623 SEQ ID NO 188 glnEΔAR-2 with flanking 500 b.p. glnE gene from 1673 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain; included 500 bp flanking the glnE gene above and below ds1133 No 1021-1623 SEQ ID NO 189 ΔnifL::Prm7 starting from 24 b.p. after A start codon ATG, 1375 bp nifL are deleted and replaced with the Prm7 promoter sequence 1021 ds1148 No 1021-1623 SEQ ID NO 190 ΔnifL::Prm7 with flanking 500 b.p. starting from 24 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced by the rm7 1021 promoter sequence; included 500 bp flanking the nifL gene above and below ds1148 No 137-1034 SEQ ID NO 191 glnEΔAR-2 glnE gene from 1290 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain ds809 No 137-1034 SEQ ID NO 192 glnEΔAR-2 with flanking 500 b.p. glnE gene from 1290 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain; included 500 bp flanking the glnE gene above and below ds809 No 137-1036 SEQ ID NO 193 ΔnifL::PinfC starting from 24 b.p. after A start codon ATG, 1372 b.p. nifL removed and replaced with 137 PinfC promoter sequence ds799 No 137-1036 SEQ ID NO 194 ΔnifL::PinfC with flanking 500 b.p. starting from 24 b.p. after A start codon ATG, 1372 b.p. nifL removed and replaced with 137 PinfC promoter sequence; included 500 bp flanking the nifL gene above and below ds799 No 137-1314 SEQ ID NO 195 glnEΔAR-2 deletion 36 bp glnE gene from 1290 bp immediately downstream of the ATG start codon, AND 36 bp is removed. removed starting from 1472 b.p. below the start codon, resulting in a truncated glnE protein lacking the adenylyl-clearing (AR) domain No No 137-1314 SEQ ID NO 196 glnEΔAR-2 deletion 36 bp glnE gene from 1290 bp immediately downstream of the ATG start codon, AND 36 bp is removed. removed starting from 1472 b.p. below the start codon, resulting in a truncated glnE protein with no adenylyl-removal (AR) domain; included 500 bp flanking the nifL gene above and below No No 137-1314 SEQ ID NO 197 ΔnifL::Prm8.2 starting from 24 b.p. after A start codon ATG, 1372 b.p. nifL were deleted and replaced with the Prm8.2 137 promoter sequence ds857 No 137-1314 SEQ ID NO 198 ΔnifL::Prm8.2 with flanking 500 b.p. starting from 24 b.p. after A start codon ATG, 1372 b.p. nifL deleted and replaced with the promoter sequence Prm8.2 137; included 500 bp flanking the nifL gene above and below ds857 No 137-1329 SEQ ID NO 199 glnEΔAR-2 deletion 36 bp glnE gene from 1290 bp immediately downstream of the ATG start codon, AND 36 bp is removed. removed starting from 1472 b.p. below the start codon, resulting in a truncated glnE protein lacking the adenylyl-clearing (AR) domain No No 137-1329 SEQ ID NO 200 glnEΔAR-2 deletion 36 bp glnE gene from 1290 bp immediately downstream of the ATG start codon, AND 36 bp is removed. removed starting from 1472 b.p. below the start codon, resulting in a truncated glnE protein with no adenylyl-removal (AR) domain; included 500 bp flanking the nifL gene above and below No No 137-1329 SEQ ID NO 201 ΔnifL::Prm6.2 starting from 24 b.p. after A start codon ATG, 1372 b.p. nifL were deleted and replaced with the Prm6.2 137 promoter sequence ds853 No 137-1329 SEQ ID NO 202 ΔnifL::Prm6.2 with flanking 500 b.p. starting from 24 b.p. after A start codon ATG, 1372 b.p. nifL deleted and replaced with the promoter sequence Prm6.2 137; included 500 bp flanking the nifL gene above and below ds853 No 137-1382 SEQ ID NO 203 ΔnifL::Prm1.2 starting from 24 b.p. after A start codon ATG, 1372 b.p. nifL were deleted and replaced with the Prm1.2 promoter sequence 137 ds843 No 137-1382 SEQ ID NO 204 ΔnifL::Prm1.2 with flanking 500 b.p. starting from 24 b.p. after A start codon ATG, 1372 b.p. nifL deleted and replaced with the promoter sequence Prm1.2 137; included 500 bp flanking the nifL gene above and below ds843 No 137-1382 SEQ ID NO 205 glnEΔAR-2 deletion 36 bp glnE gene from 1290 bp immediately downstream of the ATG start codon, AND 36 bp is removed. removed starting from 1472 b.p. below the start codon, resulting in a truncated glnE protein lacking the adenylyl-clearing (AR) domain No No 137-1382 SEQ ID NO 206 glnEΔAR-2 deletion 36 bp glnE gene from 1290 bp immediately downstream of the ATG start codon, AND 36 bp is removed. removed starting from 1472 b.p. below the start codon, resulting in a truncated glnE protein with no adenylyl-removal (AR) domain; included 500 bp flanking the nifL gene above and below No No 137-1586 SEQ ID NO 207 ΔnifL::PinfC starting from 24 b.p. after A start codon ATG, 1372 b.p. nifL removed and replaced with 137 PinfC promoter sequence ds799 No 137-1586 SEQ ID NO 208 ΔnifL::PinfC with flanking 500 b.p. starting from 24 b.p. after A start codon ATG, 1372 b.p. nifL removed and replaced with 137 PinfC promoter sequence; included 500 bp flanking the nifL gene above and below ds799 No 137-1586 SEQ ID NO 209 glnEΔAR-2 glnE gene from 1290 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain ds809 No 137-1586 SEQ ID NO 210 glnEΔAR-2 with flanking 500 b.p. glnE gene from 1290 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain; included 500 bp flanking the glnE gene above and below ds809 No 19-594 SEQ ID NO 211 glnEΔAR-2 glnE gene from 1650 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain ds34 No 19-594 SEQ ID NO 212 glnEΔAR-2 with flanking 500 b.p. glnE gene from 1650 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain; included 500 bp flanking the glnE gene above and below ds34 No 19-594 SEQ ID NO 213 ΔnifL::Prm6.1 starting from 221 b.p. after A start codon ATG, 845 bp nifL are deleted and replaced by the Prm6.1 CI019 promoter sequence ds180 No 19-594 SEQ ID NO 214 ΔnifL::Prm6.1 with flanking 500 b.p. starting from 221 b.p. after A start codon ATG, 845 bp nifL removed and replaced with CI019 Prm6.1 promoter sequence; included 500 bp flanking the nifL gene above and below ds180 No 19-714 SEQ ID NO 215 ΔnifL::Prm6.1 starting from 221 b.p. after A start codon ATG, 845 bp nifL are deleted and replaced by the Prm6.1 CI019 promoter sequence ds180 No 19-714 SEQ ID NO 216 ΔnifL::Prm6.1 with flanking 500 b.p. starting from 221 b.p. after A start codon ATG, 845 bp nifL removed and replaced with CI019 Prm6.1 promoter sequence; included 500 bp flanking the nifL gene above and below ds180 No 19-715 SEQ ID NO 217 ΔnifL::Prm7.1 starting from 221 b.p. after A start codon ATG, 845 bp nifL are deleted and replaced with the Prm7.1 CI019 promoter sequence ds181 No 19-715 SEQ ID NO 218 ΔnifL::Prm7.1 with flanking 500 b.p. starting from 221 b.p. after A start codon ATG, 845 bp nifL removed and replaced with CI019 Prm76.1 promoter sequence; included 500 bp flanking the nifL gene above and below ds181 19-713 19-750 SEQ ID NO 219 ΔnifL::Prm1.2 starting from 221 b.p. after A start codon ATG, 845 bp nifL are deleted and replaced by the Prm1.2 CI019 promoter sequence ds172 19-713 19-750 SEQ ID NO 220 ΔnifL::Prm1.2 with flanking 500 b.p. starting from 221 b.p. after A start codon ATG, 845 bp nifL were deleted and replaced with the Prm1.2 CI019 promoter sequence; included 500 bp flanking the nifL gene above and below ds172 17-724 19-804 SEQ ID NO 221 ΔnifL::Prm1.2 starting from 221 b.p. after A start codon ATG, 845 bp nifL are deleted and replaced by the Prm1.2 CI019 promoter sequence ds172 17-724 19-804 SEQ ID NO 222 ΔnifL::Prm1.2 with flanking 500 b.p. starting from 221 b.p. after A start codon ATG, 845 bp nifL were deleted and replaced with the Prm1.2 CI019 promoter sequence; included 500 bp flanking the nifL gene above and below ds172 17-724 19-804 SEQ ID NO 223 glnEΔAR-2 glnE gene from 1650 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain ds34 17-724 19-804 SEQ ID NO 224 glnEΔAR-2 with flanking 500 b.p. glnE gene from 1650 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain; included 500 bp flanking the glnE gene above and below ds34 19-590 19-806 SEQ ID NO 225 ΔnifL::Prm3.1 starting from 221 b.p. after A start codon ATG, 845 bp nifL deleted and replaced by the Prm3.1 CI019 promoter sequence ds175 19-590 19-806 SEQ ID NO 226 ΔnifL::Prm3.1 with flanking 500 b.p. starting from 221 b.p. after A start codon ATG, 845 bp nifL were deleted and replaced with the Prm3.1 CI019 promoter sequence; included 500 bp flanking the nifL gene above and below ds175 19-590 19-806 SEQ ID NO 227 glnEΔAR-2 glnE gene from 1650 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain ds34 19-590 19-806 SEQ ID NO 228 glnEΔAR-2 with flanking 500 b.p. glnE gene from 1650 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain; included 500 bp flanking the glnE gene above and below ds34 No 63-1146 SEQ ID NO 229 ΔnifL::PinfC starting from 24 b.p. after A start codon ATG, 1375 bp nifL removed and replaced with 63 PinfC promoter sequence ds908 No 63-1146 SEQ ID NO 230 ΔnifL::PinfC with flanking 500 b.p. starting from 24 b.p. after A start codon ATG, 1375 bp nifL removed and replaced with 63 PinfC promoter sequence; included 500 bp flanking the nifL gene above and below ds908 CM015; PBC6.15 6-397 SEQ ID NO 231 ΔnifL::Prm5 starting from 31 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the Prm5 CI006 promoter sequence ds24 CM015; PBC6.15 6-397 SEQ ID NO 232 ΔnifL::Prm5 with flanking 500 b.p. starting from 31 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the Prm5 CI006 promoter sequence; included 500 bp flanking the nifL gene above and below ds24 CM014 6-400 SEQ ID NO 233 ΔnifL::Prm1 starting from 31 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the Prm1 CI006 promoter sequence ds20 CM014 6-400 SEQ ID NO 234 ΔnifL::Prm1 with flanking 500 b.p. starting from 31 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the Prm1 CI006 promoter sequence; included 500 bp flanking the nifL gene above and below ds20 CM037; PBC6.37 6-403 SEQ ID NO 235 ΔnifL::Prm1 starting from 31 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the Prm1 CI006 promoter sequence ds20 CM037; PBC6.38 6-403 SEQ ID NO 236 ΔnifL::Prm1 with flanking 500 b.p. starting from 31 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the Prm1 CI006 promoter sequence; included 500 bp flanking the nifL gene above and below ds20 CM037; PBC6.39 6-403 SEQ ID NO 237 glnEΔAR-2 glnE gene from 1644 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain ds31 CM037; PBC6.40 6-403 SEQ ID NO 238 glnEΔAR-2 with flanking 500 b.p. glnE gene from 1644 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain; included 500 bp flanking the glnE gene above and below ds31 CM038; PBC6.38 6-404 SEQ ID NO 239 glnEΔAR-1 glnE gene from 1287 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain ds30 CM038; PBC6.38 6-404 SEQ ID NO 240 ΔnifL::Prm1 starting from 31 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the Prm1 CI006 promoter sequence ds20 CM038; PBC6.38 6-404 SEQ ID NO 241 ΔnifL::Prm1 with flanking 500 b.p. starting from 31 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the Prm1 CI006 promoter sequence; included 500 bp flanking the nifL gene above and below ds20 CM038; PBC6.38 6-404 SEQ ID NO 242 glnEΔAR-1 with flanking 500 b.p. glnE gene from 1287 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain; included 500 bp flanking the glnE gene above and below ds30 CM029; PBC6.29 6-412 SEQ ID NO 243 glnEΔAR-1 glnE gene from 1287 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain ds30 CM029; PBC6.29 6-412 SEQ ID NO 244 glnEΔAR-1 with flanking 500 b.p. glnE gene from 1287 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain; included 500 bp flanking the glnE gene above and below ds30 CM029; PBC6.29 6-412 SEQ ID NO 245 ΔnifL::Prm5 starting from 31 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the Prm5 CI006 promoter sequence ds24 CM029; PBC6.29 6-412 SEQ ID NO 246 ΔnifL::Prm5 with flanking 500 b.p. starting from 31 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the Prm5 CI006 promoter sequence; included 500 bp flanking the nifL gene above and below ds24 CM093; PBC6.93 6-848 SEQ ID NO 247 ΔnifL::Prm1 starting from 31 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the Prm1 CI006 promoter sequence ds20 CM093; PBC6.93 6-848 SEQ ID NO 248 ΔnifL::Prm1 with flanking 500 b.p. starting from 31 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the Prm1 CI006 promoter sequence; included 500 bp flanking the nifL gene above and below ds20 CM093; PBC6.93 6-848 SEQ ID NO 249 glnEΔAR-2 glnE gene from 1644 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain ds31 CM093; PBC6.93 6-848 SEQ ID NO 250 glnEΔAR-2 with flanking 500 b.p. glnE gene from 1644 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain; included 500 bp flanking the glnE gene above and below ds31 CM093; PBC6.93 6-848 SEQ ID NO 251 ΔamtB The first 1088 b.p. amtB gene and 4 b.p. above start codon removed; 199 b.p. the gene residue lacks a start codon; translation of the amtB protein does not occur ds126 CM093; PBC6.93 6-848 SEQ ID NO 252 ΔamtB with flanking 500 b.p. The first 1088 b.p. amtB gene and 4 b.p. above start codon removed; 199 b.p. the gene residue lacks a start codon; translation of the amtB protein does not occur ds126 CM094; PBC6.94 6-881 SEQ ID NO 253 glnEΔAR-1 glnE gene from 1287 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain ds30 CM094; PBC6.94 6-881 SEQ ID NO 254 glnEΔAR-1 with flanking 500 b.p. glnE gene from 1287 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain; included 500 bp flanking the glnE gene above and below ds30 CM094; PBC6.94 6-881 SEQ ID NO 255 ΔnifL::Prm1 starting from 31 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the Prm1 CI006 promoter sequence ds20 CM094; PBC6.94 6-881 SEQ ID NO 256 ΔnifL::Prm1 with flanking 500 b.p. starting from 31 b.p. after A start codon ATG, 1375 bp nifL deleted and replaced with the Prm1 CI006 promoter sequence; included 500 bp flanking the nifL gene above and below ds20 CM094; PBC6.94 6-881 SEQ ID NO 257 ΔamtB The first 1088 b.p. amtB gene and 4 b.p. above start codon removed; 199 b.p. the gene residue lacks a start codon; translation of the amtB protein does not occur ds126 CM094; PBC6.94 6-881 SEQ ID NO 258 ΔamtB with flanking 500 b.p. The first 1088 b.p. amtB gene and 4 b.p. above start codon removed; 199 b.p. the gene residue lacks a start codon; translation of the amtB protein does not occur ds126 No 910-1246 SEQ ID NO 259 ΔnifL::PinfC starting from 20 b.p. after A start codon ATG, 1379 b.p. nifL removed and replaced with 910 PinfC promoter sequence ds960 No 910-1246 SEQ ID NO 260 ΔnifL::PinfC with flanking 500 b.p. starting from 20 b.p. after A start codon ATG, 1379 b.p. nifL removed and replaced with 910 PinfC promoter sequence; included 500 bp flanking the nifL gene above and below ds960 PBC6.1, 6, CI6 CI006 SEQ ID NO 261 16S-1 1 of 3 unique 16S rDNA genes in the CI006 genome n.p. PBC6.1, 6, CI6 CI006 SEQ ID NO 262 16S-2 2 of 3 unique 16S rDNA genes in the CI006 genome n.p. PBC6.1, 6, CI6 CI006 SEQ ID NO 263 nifH n.p. n.p. PBC6.1, 6, CI6 CI006 SEQ ID NO 264 nifD2 2 of 2 unique genes annotated in the CI006 genome as nifD n.p. PBC6.1, 6, CI6 CI006 SEQ ID NO 265 nifK2 2 of 2 unique genes annotated in the CI006 genome as nifK n.p. PBC6.1, 6, CI6 CI006 SEQ ID NO 266 nifL n.p. n.p. PBC6.1, 6, CI6 CI006 SEQ ID NO 267 nifA n.p. n.p. PBC6.1, 6, CI6 CI006 SEQ ID NO 268 glnE n.p. n.p. PBC6.1, 6, CI6 CI006 SEQ ID NO 269 16S-3 3 of 3 unique 16S rDNA genes in the CI006 genome n.p. PBC6.1, 6, CI6 CI006 SEQ ID NO 270 nifD1 1 of 2 unique genes annotated in the CI006 genome as nifD n.p. PBC6.1, 6, CI6 CI006 SEQ ID NO 271 nifK1 1 of 2 unique genes annotated in the CI006 genome as nifK n.p. PBC6.1, 6, CI6 CI006 SEQ ID NO 272 amtB n.p. n.p. PBC6.1, 6, CI6 CI006 SEQ ID NO 273 Prm1 348 b.p. includes 319 b.p. immediately upstream of the ATG start codon of the lpp gene and the first 29 b.p. lpp gene n.p. PBC6.1, 6, CI6 CI006 SEQ ID NO 274 Prm5 313 b.p. starting from 432 b.p. above the ATG start codon of the ompX gene and ending at 119 bp. upstream of the ATG start codon of the ompX gene n.p. 19, CI19 CI019 SEQ ID NO 275 nifL n.p. n.p. 19, CI19 CI019 SEQ ID NO 276 nifA n.p. n.p. 19, CI19 CI019 SEQ ID NO 277 16S-1 1 of 7 unique 16S rDNA genes in the CI019 genome n.p. 19, CI19 CI019 SEQ ID NO 278 16S-2 2 of 7 unique 16S rDNA genes in the CI019 genome n.p. 19, CI19 CI019 SEQ ID NO 279 16S-3 3 of 7 unique 16S rDNA genes in the CI019 genome n.p. 19, CI19 CI019 SEQ ID NO 280 16S-4 4 of 7 unique 16S rDNA genes in the CI019 genome n.p. 19, CI19 CI019 SEQ ID NO 281 16S-5 5 of 7 unique 16S rDNA genes in the CI019 genome n.p. 19, CI19 CI019 SEQ ID NO 282 16S-6 6 of 7 unique 16S rDNA genes in the CI019 genome n.p. 19, CI19 CI019 SEQ ID NO 283 16S-7 7 of 7 unique 16S rDNA genes in the CI019 genome n.p. 19, CI19 CI019 SEQ ID NO 284 nifH1 1 of 2 unique genes annotated in the CI019 genome as nifH n.p. 19, CI19 CI019 SEQ ID NO 285 nifH2 2 of 2 unique genes annotated in the CI019 genome as nifH n.p. 19, CI19 CI019 SEQ ID NO 286 nifD1 1 of 2 unique genes annotated in the CI019 genome as nifD n.p. 19, CI19 CI019 SEQ ID NO 287 nifD2 2 of 2 unique genes annotated in the CI019 genome as nifD n.p. 19, CI19 CI019 SEQ ID NO 288 nifK1 1 of 2 unique genes annotated in the CI019 genome as nifK n.p. 19, CI19 CI019 SEQ ID NO 289 nifK2 2 of 2 unique genes annotated in the CI019 genome as nifK n.p. 19, CI19 CI019 SEQ ID NO 290 glnE n.p. n.p. 19, CI19 CI019 SEQ ID NO 291 Prm4 449 b.p. immediately upstream of the ATG of the dscC2 gene n.p. 19, CI19 CI019 SEQ ID NO 292 Prm1.2 500 b.p. immediately upstream of the TTG start codon of the infC gene n.p. 19, CI19 CI019 SEQ ID NO 293 Prm3.1 170 bp immediately upstream of the ATG start codon of the rplN gene n.p. 19, CI20 CI020 SEQ ID NO 294 Prm6.1 142 b.p. immediately upstream of the ATG of a highly expressed hypothetical protein (annotated as PROKKA_00662 in assembly 82 CI019) n.p. 19, CI21 CI021 SEQ ID NO 295 Prm7.1 293 b.p. immediately upstream of the ATG of the lpp gene n.p. 19-375, 19-417, CM067 CM67 SEQ ID NO 296 glnEΔAR-2 glnE gene from 1650 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain ds34 19-375, 19-417, CM067 CM67 SEQ ID NO 297 glnEΔAR-2 with flanking 500 b.p. glnE gene from 1650 bp immediately downstream of the start codon, ATG is deleted, resulting in a truncated glnE protein with no adenylyl-deleting (AR) domain; included 500 bp flanking the glnE gene above and below ds34 19-375, 19-417, CM067 CM67 SEQ ID NO 298 ΔnifL::null-v1 starting from 221 b.p. after A start codon ATG, 845 bp nifL removed and replaced by 31 b.p. sequence "GGAGTCTGAACTCATCCTGCGATGGGGGCTG" No 19-375, 19-417, CM067 CM67 SEQ ID NO 299 ΔnifL::null-v1 with flanking 500 b.p. starting from 221 b.p. after A start codon ATG, 845 bp nifL removed and replaced by 31 b.p. sequence "GGAGTCTGAACTCATCCTGCGATGGGGGCTG"; included 500 bp flanking the nifL gene above and below No 19-377, CM069 CM69 SEQ ID NO 300 ΔnifL::null-v2 starting from 221 b.p. after A start codon ATG, 845 bp nifL removed and replaced with 5 b.p. sequence "TTAAA" No 19-377, CM069 CM69 SEQ ID NO 301 ΔnifL::null-v2 with flanking 500 bp starting from 221 b.p. after A start codon ATG, 845 bp nifL removed and replaced with 5 b.p. the sequence "TTAAA"; included 500 bp flanking the nifL gene above and below No 19-389, 19-418, CM081 CM81 SEQ ID NO 302 ΔnifL::Prm4 starting from 221 b.p. after A start codon ATG, 845 bp nifL deleted and replaced with Prm4 CI19 sequence ds70 19-389, 19-418, CM081 CM81 SEQ ID NO 303 ΔnifL::Prm4 with flanking 500 b.p. starting from 221 b.p. after A start codon ATG, 845 bp nifL removed and replaced with CI19 Prm4 sequence; included 500 bp flanking the nifL gene above and below ds70

Table H

SEQID NO: Subsequence 61 atgtttaacgatctgattggcgatgatgaaacggattcgccggaagatgcgctttctgagagctggcgcgaattgtggcaggatgcgttgcaggaggaggattccacgcccgtgctggcgcatctctcagaggacgatcgccgccgcgtggtggcgctgattgccgattttcgcaaagagttggataaacgcaccatt ggccgcgagggcggcaggtactcgatcacttaatgccgcatctgctcagcgatgtatgctcgcgcgcgacgatgcgccagtaccgctgtcacgcctgacgccgctgctcaccggaattattacccgcaccacttaccttgagctgctaagtgaatttcccggcgcactgaaacacctcatttccctgtgtgccgcgt cgccgatggttgccagtcagctggcgcgctacccgatcctgcttgaatgaattgctcgacccgaatacgctctatcaaccgacggcgatgaatgcctatcgcgatgagctgcgccaatacctgctgcgcgtgccggaagatgatgaagagcaacagcttgaggcgctgcggcagtttaagcaggcgcagttgct gcgcgtggcggcggcggatattgccggtacgttgccagtaatgaaagtgagcgatcacttaacctggctggcggaagcgattattgatgcggtggtgcagcaagcctggggggcagatggtggcgcgttatggccagccaacgcatctgcacgatcgcgcgaagggcgcggttttgcggtggtcggttatggcaagctggg cggctgggagctgggttacagctccgatctggatctggtattcctgcacgactgcccgatggatgtgatgaccgatggcgagcgtgaaatcgatggtcgccagttctatttgcgtctcgcgcagcgcgtgatgcacctgtttagcacgcgcacgtcgtccggcatcctttatgaagttgatgcgcgt ctgcgtccatctggcgctgcggggatgctggtcactactacggaatcgttcgccgattaccagcaaaaacgaagcctggacgtgggaacatcaggcgctggcccgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatattctgatgacgcctcgcgacggcgcaac gctgcaaaccgacgtgcgagaaatgcgcgagaaaatgcgtgcccatcttggcaacaagcataaagaccgcttcgatctgaaagccgatgaaggcggtatcaccgacatcgagtttatcgcccaatatctggtgctgcgctttgcccatgacaagccgaaactgacgcgctggtcggataatgtgcgcattctcga agggctggcgcaaaacggcatcatggaggcaggaagcgcaggcattgacgctggcgtacaccacattgcgtgatgagctgcaccacctggcgctgcaagagttgccgggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttattaaaaccagctgggacaagtggctggtggaaccgtgcgccccggc gtaa 62 ttgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgaacggtagcacagagagcttgctctcgggtgacgagtggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataccgcataacgtcgcaagaccaaagagggggaccttcgggcctctt gccatcagatgtgcccagatgggattagctagtaggtggggtaacggctcacctaggcgacgatccctagctggtctgaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtgtgaagaaggccttcgggttgta aagcactttcagcggggaggaagggagtaaggttaataaccttattcattgacgttacccgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggtctgtcaagtcggatgtgaaatccccgggctcaacctgggaactgcatccgaa actggcaggcttgagtctcgtagagggaggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggcctcctggacgaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtctatttggaggttgtgcccttgaggcgt ggcttccggagctaacgcgttaaatagaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctggtcttgacatccacagaactttccagagatggattggtgccttcgggaactgtgagacaggtgctgcatggctg tcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatcctttgttgccagcggtccggccgggaactcaaaggagactgccagtgataaactggaggaaggtggggatgacgtcaagtcatcatggcccttacgaccagggctacacacgtgctacaatggcgcatacaaagagaagcgacctc gcgagagtaagcggacctcataaagtgcgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtggatcagaatgccacggtgaatacgttcccggggccttgtacacaccgcccgtcacaccatgggagtgggttgcaaaagaagtaggtagcttaaccttcgggagggcgcttaccact ttgtgattcatgactggggtgaagtcgtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 63 ttgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgaacggtagcacagagagcttgctctcgggtgacgagtggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataccgcataacgtcgcaagaccaaagagggggaccttcgggcctctt gccatcagatgtgcccagatgggattagctagtaggtggggtaacggctcacctaggcgacgatccctagctggtctgaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtgtgaagaaggccttcgggttgta aagcactttcagcggggaggaaggnantanggttaataacctgtgttnattgacgttacccgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggtctgtcaagtcggatgtgaaatccccgggctcaacctgggaactgcatccga aactggcaggcttgagtctcgtagagggaggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggcctcctggacgaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtctatttggaggttgtgcccttgaggcg tggcttccggagctaacgcgttaaatagaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctggtcttgacatccacagaacttagcagagatgctttggtgccttcgggaactgtgagacaggtgctgcatggct gtcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatcctttgttgccagcggttaggccgggaactcaaaggagactgccagtgataaactggaggaaggtggggatgacgtcaagtcatcatggcccttacgaccagggctacacacgtgctacaatggcgcatacaaagagaagcgacct cgcgagagtaagcggacctcataaagtgcgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtggatcagaatgccacggtgaatacgttcccggggccttgtacacaccgccgtcacaccatgggagtgggttgcaaaagaagtaggtagcttaaccttcgggagggcgcttacc actttgtgattcatgactggggtgaagtcgtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 64 attgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgagcggcancgggaagtagcttgctactttgccggcgagcggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataatccgcatgacctcgaaaagagcaaagtgggggatcttcggacctc . aagcactttcagcgaggaggaaggcanacttaatacgtgtgntgattgacgttactcgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggttgttaagtcagatgtgaaatccccgagcttaacttgggaactgcatttga aactggcaagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgctgtaaacgatgtcgacttggaggttgtgcccttgaggcg tggcttccggagctaacgcgttaagtcgaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctactcttgacatccagagaatttgccagagatggcgaagtgccttcgggaactctgagacaggtgctgcatggct gtcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatcctttgttgccagcacgtnatggtgggaactcaaaggagactgccggtgataaaccggaggaaggtggggatgacgtcaagtcatcatggcccttacgagtagggctacacacgtgctacaatggcatatacaaagagaagcgaact cgcgagagcaagcggacctcataaagtatgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtagatcagaatgctacggtgaatacgttcccgggccttgtacacaccgcccgtcacaccatgggagtgggttgcaaaagaagtaggtagcttaaccttcgggagggcgcttaccacttt gtgattcatgactggggtgaagtcgtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 65 atgaccatgcgtcaatgcgccatttacggcaaaggtgggatcggcaaatcgaccaccacacagaacctggtcgccgcgctggcggagatgggtaaaaaagtcatgattgtcggctgtgacccgaaagccgattccacgcgtttgatcctgcatgcgaaagcgcagaacaccattatggagatggctgctgaagtcggct ccgtggaagacctggagttagaagacgtgctgcaaatcggttacggcggcgtgcgctgcgcagagtccggcggcccggagccaggcgtgggctgtgccggtcgcggggtgatcaccgcgattaacttcctcgaagaagaaggcgcttacgtgccggatctcgattttgttttctacgacgtgctgggcgacgt ggtatgcggtggtttcgccatgccgattcgtgaaaacaaagcgcaggagatctacatcgtttgctctctggcgaaatgatggcgatgtacgccgccaacaacatctccaaaggcatcgtgaaatacgccaaatccggtaaagtgcgcctcggcgggctgatttgtaactcgcgccagaccgaccgtgaagatgaactga tcattgcgctggcagaaaaactcggcacgcagatgatccactttgttccccgcgacaacattgtgcagcgtgcggaaatccgccgtatgacggttatcgaatatgacccgacctgcaatcaggcgaacgaatatcgcagccttgccagcaaaatcgtcaacaacaccaaaatggtggtgcccaccccctgcaccat ggatgaactggaagaactgctgatggagttcggcattatggatgtggaagacaccagcatcattggtaaaaccgccgccgaagaaaacgccgtctga 66 atgagcaatgcaaccaggcgaacgcaacctggagataatcgagcaggtgctcgaggttttcccggagaagacgcgcaaagaacgcagaaaacacatgatggtgacggacccggagcaggaaagcgtcggtaagtgcatcatctctaaccgcaaatcgcagccaggcgtgatgaccgtgcgcggctgctcgtatgccggtt cgaaaggggtggtatttggggccaatcaaggatatggcgcatatctcgcatggcccaatcggctgcggccaatactcccgcgccgggcggcggaactactacaccggcgtcagcggcgtggacagcttcggcacgctcaacttcacctccgattttcaggagcgcgacatcgtgtttggcggcgataaaaagctcgccaaactga ttgaagagctggaagagctgttcccgctgaccaaaggcatttcgattcagtcggaatgcccggtcggcctgattggcgatgacattgaggccgtcgcgaacgccagccgcaaagccatcaacaaaccggttattccggtgcgttgcgaaggctttcgcggcgtgtcgcaatccctcggtcaccatattgccaacgatg tgatccgcgactgggtgctggataaccgcgaaggcaaaccgttcgaatccaccccttacgatgtggcgatcatcggcgattacaaacatcggcggcgatgcctgggcttcgcgcattttgctcgaagatgggcttgcgggtggtggcacagtggtctggcgacggtacgctggtggagatggaaaacacgccg ttcgtcaaactgaacctggtgcattgttaccgtgcattgttaccgctcaatgaactacatctcgcgccatatggaggagaagcacggtattccgtggatggaatacaacttctttggtccgacgaaaatcgcggaatcgctgcgcaaaatcgccgaccagtttgacgacaccattcgcgccaacgccgaagcggtgatcgccagataccaggcgcaa aacgacgccattatcgccaaatatcgcccgcgtctggaggggcgcaaagtgctgctttatatgggcgggctgcgtccgcgccatgtgattggcgcctatgaagacctgggaatggagatcatcgctgccggttatgagttcggtcataacgatgattacgaccgcaccttgccggatctgaaagagggcacgctgctgt ttgatgatgccagcagttatgagctggaggcgttcgtcaacgcgctgaaaccggatctcatcggttccggcatcaaagaagtacatctttcagaaaatgggcgtgccgtttcgccagatgcactcctgggattactccggcccgtaccacggctatgacggcttcgccatcttcgcccgcgatatggatatgacgctcaaca accccgcgtggggccagttgaccgcgccgtggctgaaatccgcctga 67 atgagccagactgctgagaaaatacagaattgccatcccctgtttgaacaggatgcttaccagacgctgttgccggtaaacgggcactcgaagaggcgcactcgccggagcgggtgcaggaagtgtttcaatggaccactaccccggaatatgaagcgctgaactttaaacgcgaagcgctgactatcgacccggcaaaagcc tgccagccgctgggcgcggtgctctgttcgctggggtttgccaataccctaccgtatgtgcacggttcacagggttgcgtggcctatttccgcacgtactttaaccgccactttaaagaaccggtggcctgcgtgtcggattcaatgacggaagacgcggcggtgttcggcgggaataaccctcaacaccgg cttacaaaacgccagcgcgctgtataaaccggagattatcgccgtctctaccacctgtatggcggaagtgatcggtgatgatttgcaggcctttcgccaacgccaaaaaagatggttttctcgatgccgccatccccgtgccctacgcgcacacccccagttttatcggcagccatatcaccggctgggataacatgt ttgaaggttttgcccggaccttttacggcagaccatgaagctcagccggcaaactttcacgcatcaacctggtgaccgggtttgaaacctatctcggcaatttccgcgtgctgaaacgcatgatggaacaaatggaggtgccggcgagtgtgctctccgatccgtcggaagtgctggatactccgccaacgggcattaccagatgt acgcgggcgggacgacgcagcaagagatgcgcgaggcgccggatgctatcgacaccctgttgctgcagccctggcaactggtgaaaagcaaaaaagtggtgcaggagatgtggaatcagcccgccaccgaggttctgttcccgttgggctggcaggaacagacgaactgttgatggcgattagccagttaaccggcaagg ccattcccgattcactggcgctggagcgcgggcggctggtcgatatgatgctcgattcccacacctggttgcacggtaaaaaattcggcctgtttggcgatccggatttgtcatgggattgacccgtttcctgctggagctgggctgcgaaccgaccgttatcctctgccacaacggtaacaaacgctggcagaaagcaat gaagaaaatgcttgacgcctcgccgtacggccaggagagcgaagtgtttatcaactgcgatttgtggcatttccgctcgctgatgtttacccgccagccggattttatgattggcaactcgtacggcaagttcattcagcgcgacaccttagccaaaggcgagcagtttgaagttccgctgatccgcctcggttttcccctgtt cgaccgccaccatctgcaccgccagaccacctggggctacgaggccgccatgagcattctcactacccttgtgaatgcggtactggagaaagtggacaaagagaccatcaagctcggcaaaaccgactacagcttcgatcttatccgttaa 68 atgaccctgaatatgatgatggatgccggcgcgcccgaggcaatcgccggtgcgctttcgcgacaccatcctgggctgttttttaccatcgttgaagaagcgcccgtcgccatttcgctgactgatgccgacgcacgcattgtctatgccaacccggctttctgccgccagaccggctatgaactagaagcgttgttgcagca aaatccccgcctgcttgcaagtcgccaaaccccacgggaaatctatcaggatatgtggcacaccttgttacaacgccgaccgtggcgcgggcaattgattaaccgccaccgcgacggcagcctgtatctggtcgagatcgatatcaccccggtgattaacccgtttggcgaactggaacactacctggcaatgcagcgcgata tcagcgccagttatgcgctggagcagcggttgcgcaatcacatgacgctgaccgaagcggtgctgaataacattccggcggcggtggttgtagtggatgaacgcgatcatgtggttatggataaccttgcctacaaaacgttctgtgccgactgcggcggaaaagagctcctgagcgaactcaatttttcagcccgaaaagc ggagctggcaaacggccaggtcttaccggtggtgctgcgcggtgaggtgcgctggttgtcggtgacctgctgggcgctgccgggcgtcagcgaagaagccagtcgctactttattgataacaggctgacgcgcacgctggtggtgatcaccgacgacacccaacaacgccagcagcaggaacagggccgacttgaccg ccttaaacagcagatgaccaacggcaaactactggcagcgatccgcgaagcgcttgacgccgcgctgatccagcttaactgccccatcaatatgctggcggcggcgcgacgtttaaacggcagtgataacaacaatgtggcgctcgacgccgcgtggcgcgaaggtgaagaggcgatggcgcggctgaaacgttg ccgcccgtcgctggaactggaaagtgcggccgtctggccgctgcaacccttttttgacgatctgcgcgcgcttttcacacccgctacgagcagggggaaaaatttgcaggtcacgctggattcccatcatctggtgggatttggtcagcgtacgcaactgttagcctgcctgagtctgtggctcgatcgcac gctggatattgccgccgggctgggtgatttcaccgcgcaaacgcagatttacgcccgcgaagaagagggctggctctctttgtatatcactgacaatgtgccgctgatcccgctgcgccacacccactcgccggatgcgcttaacgctccgggaaaaggcatggagctgcgcctgatccagacgctggtggcacaccaccacggc gcaatagaactcacttcacaccccgaagggggaagttgcctgaccctacgattcccgctatttcattcactgaccgggaggttcaaaatga 69 atgacccagcgaaccgagtcgggtaataccgtctggcgcttcgatttgtcccagcagttcactgcgatgcagcgcataagcgtggtactcagccgggcgaccgaggtcgatcagacgctccagcaagtgctgtgcgtattgcacaatgacgcctttttgcagcacggcatgatctgtctgtacgacagcca gcaggcgattttgaatattgaagcgttgcaggaagccgatcagcagttaatccccggcagctcgcaaatccgctatcgtccgggcgaagggctggtcgggacggtgctttcgcaggccaatcattagtgctggcgcgcgttgctgacgatcagcgctttcttgaccggctcgggttgtatgattacaacctgccgtttat cgccgtgccgctgatagggccagatgcgcagactttcggtgtgctgacggcacaacccatggcgcgttacgaagagcgattacccgcctgcacccgctttctggaaacggtcgctaacctggtcgcgcaaaccgtgcgttgatggcaccaccggcagtgcgcccttccccgcgcgccgccataacacagg ccgccagcccgaaatcctgcacggcctcacgcgcatttggttttgaaaatatggtcggtaacagtccggcgatgcgccagaccatggagattatccgtcaggtttcgcgctgggacaccaccgttctggtacgcggcgagtggcaccggcaaggagctgattgccaacgccatccaccaccattcgccgcgtgccggtgcgcca tttgtgaaattcaactgtgcggcgctgccggacacactgctggaaagcgaattgttcggtcacgagaaaggggcatttaccggcgcggtacgccagcgtaaaggccgttttgagctggccgatggcggcacgctgtttcttgacgagatcggcgagtagcgcctcgtttcaggctaagctgctgcgcatt ttgcaggaaggcgaaatggaacgcgtcggcggcgacgagacattgcaagtgaatgtgcgcattattgccgcgacgaaccgcaatcttgaagatgaagtccggctggggcactttcgcgaagatctctattatcgcctgaatgtgatgcccatcgccctgccgccactacgcgaacgccaggaggacattgccgagctggcg cactttctggtgcgtaaaatcgcccataaccagagccgtacgctgcgcattagcgagggcgctatccgcctgctgatgagctacaactggcccggtaatgtgcgcgaactggaaaactgccttgagcgctcagcggtgatgtcggagaacggtctgatcgatcgggatgtgattttgtttaatcatcgcgaccag ccagccaaaccgccagttatcagcgtctcgcatgatgataactggctcgataacaaccttgacgagcgccagcggctgattgcggcgctggaaaaagcgggatgggtacaagccaaagccgcgcgcttgctggggatgacgccgcgccaggtcgcctatcgtattcagacgatggatataaccctgccaaggctataa 70 atggcaatgcgtcaatgtgcaatctacgggaaagggggtattggtaaatccaccactacccaaaaccttgtagcggctctggccgaaatgaataagaaggtcatgatcgtcggctgtgaccctaaggctgattcaacccgcctcattctgcatgcgaaagcacagaacaccatcatggaaatggccgctgaagtgggctccgtggaagatctgg agctggaagatgtgatgcaaatcggctatggcggcgtgcgctgtgcggaatcaggcggccctgagcctggtgtgggttgtgccggacgcggggtgatcaccgccatcaacttcctcgaagaagaaggcgcgtatgtgccggatctggatttcgtgttttacgacgtattgggcgatgtggtctgtggc ggtttcgcgatgccaattcgcgaaaacaaagcgcaggaaatctacatcgtatgctccggtgaaatgatggcgatgtatgccgccaacaacatttccaaaggcatcgtgaaatacgcgaaatcgggcaaagttcgcctggccgggctgatctgtaactccgccagacggatcgcgaagatgaactgatcatcgc gctggctgaaaaacttggcacgcaaatgatccacttcgtgccgcgtgacaacattgtgcaacgcgctgaaatccgccgcatgacggtcatcgaatacgacccgacttgtgcgcaggcagatcagtatcgtgcactggcgaacaaaatcgtcaacaacaccaaaatggtggtgccgacaccggtcaccatgg atgagctggaagccctgttaatggaatttggcattatggaagaagaagacctgaccatcgtcggtcgtaccgccgccgaagaggcgtga 71 atgaccagtgaaacacgcgaacgtaacgaggcattgatccaggaagtgctggagatcttccccgagaaggcgcttaaagatcgtaagaaacacatgatgaccaccgacccggcgatggaatctgtcggcaagtgtattgtctcaaaccgcaaatcacagccgggcgtgatgaccgtgcgaggctgcgcttacgccggtt ccaaaggcgtggtctttggcccgatcaaagacatggcgcatatctcccacggcccggttggttgcggccagtattctcgtgccggacgccgtaactattacaccggctggagcggcgtgaacagctttggcaccctcaacttcaccagtgattttcaggaacgggacatcgtatttggcggcgataaaaagctcgacaaactgatcgacgaactgg agatgttgttcccgctgaccaaaggcatttcggtacagtcggaatgtccggtcggtctgatcggcgatgacatttctgccgtcgccaaagccagcagcgccaaaatcggtaagccggtcgtgccggtacgctgcgaggggttccgcggtgtgtcgcaatcgctcggccatcacattgctaacgatgtcatccg cgactgggtgctggataaccgcgaaggcaatgaatttgaaaccacgccttacgacgtggcgattatcggcgactacaacatcggcggtgacgcctgggcctcacgtattctgctcgaagaaatggggctgcgtgtggtggcgcagtggtccggcgacggcacgctggtggagatggaaaacaccccgaaagtc gcactcaatctggtgcactgctaccgctcgatgaactacatctcccgtcatatggaagaaaaacacggcattccgtggatggaatacaacttttggcccgaccaaaattgcggaatctctgcgcgaaatcgcggcgcgttttgacgataccatccggaaaaacgccgaagcggtgattgaaaaatatcaggcgcaaac gcaggcggtgatcgacaaataccgtccgcgtctggaaggcaaaaaggtgctgttgtatctcggcggtttacgtccgcgccacatcatcggggcgtatgaagatctgggaatggaaatcatcggtaccggctatgaattcggtcataacgatgattacgaccgcaccttaccgatgctcaaagaaggcacgttgctgttc gatgacctgagcagttatgagctggaagcgttcgttaaagcgctgaaaccggatctgtcgggtcaggcatcaaagaaaaatacattttccagaaaatgggcgtgccgttccgccagatgcactcctgggattattccggcccttatcacggctacgacggtttcggcatttttgcccgtgacatggacatgacgctgaacaat ccgggctggagtcagctgaccgccccctggttgaaatcggcctga 72 atgagtcaagatcttggcaccccaaaatcctgtttcccgctgttcgagcaggatgaataccagagtatgtttacccacaaacgcgcgctggaagaagcacacggcgaggcgaaagtgcgggaagtgtttgaatggaccaccacgcaggaatatcaggatctgaacttcgcgtgaagcgctgaccgtcgacccggcgaa agcctgccagccgttaggcgcggtactttgcgcgctgggttttgccaacacgttgccgtatgtccacggttcacaaggctgtgtggcgtatttccgtacctattttaatcgtcatttcaaagagccggtggcctgtgtttccgactcaatgaccgaagaatgccgccgttttggcggaaataacaacatgaatgtcgg tctggaaaacgccagcgcgctgtacaagccggaaattattgctgtctccaccacctgtatggcggaagtgatcggtgatgacctgcaggctttttcgccaacgccaaaaaagacggatttgtggatgccggtatgccaatcccgtatgcccatacaccgagttttctgggcagtcatgtcaccggctgggacaacatgttt gaaggcttcgcccgtacctttaccaccgacgccacgcgggaatatcagccgggcaaacttgccaaactgaacgtggtgaccggttttgaaacttatctcggcaactaccgggttattcaccgcatgatgagccagatgggggtcgaatgcagcgtcttgtccgatccgtctgaagtgctcgacaccccggctgacggccaatcccat gtatgccggcggcaccacgcaaaccgaaatgcgtgatgcaccggatgccatcgacaccttgctgctgcaaccgtggcaattgcagaaaaccaaaaaagtggtgcagggcgactggaatcagccgggcaccgaagtcagtgtaccgattggcctggcggcgaccgatgccttgctgatgacggtaagcgaactgacc ggcaaaccgatagctgacacgctggcgactgaacgtggccgtctggtggacatgatgctcgattcccacacctggctgcatggcaagcgtttcggtctctacggtgacccggattttgtgatgggcatgaccgcattcctgctggaactgggctgtgaaccgaccaccattctcagccataacggcaacaaacgctggcagaaagccatgaaga aaatgctggctgattcgccttacgggcaggacagcgaagtgtatgtgaactgcgatctgtggcatttccgctcgctgatgtttacccgtaaaccggactttatgatcggcaactcttacggaaaattcattcagcgtgacacgctggccaaaggcgaacagttcgaagtgccgctgatccgcatcggttttccgatttttga ccggcaccatttgcaccgtcagaccacctggggatacgaaggggcgatgagcatactgacgcaactggtgaatgcggtacttgaacaactggatcgcgaaaccatgaagctcggcaaaaccgactacaacttcgacctgatccgctaa 73 atgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggttgaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaac caccgtctgctggccagccagcagacgccgaaacatatctatgacgaaatgtggcgcactttgttgcaggccaaatcctggaacggccaactgatcaaccggcgtaataaccgttcgctttatctggcggatgtcactatcacgcctgttttaggcgcggacgggcaggtggagcattacctcggcatgcacaaagatatcagcg agaaatacgcgctggaacagcggttgcgcaaccacatcaccttgttcacggaggtgctgaacaatattcccgccgccgtggtggtggtggatgagcaggacaatgtggtgatggacaatctggcctacaaaaccctttgcgcggactgcggcggcaaagagctgctggctgaaatgggctatccgcaactcaaagagatgctca acagtggcgaaccggtgccggtttccatgcgcggttttctttcggtcaatggttattgcagggcgttaatgaagaggccagccgcttttttaccggcattaccgcgccgggaaaactgattgttctgaccgactgcaccgatcagcatcaccggcagcagcagggttatcttgaccggcttaagcaaaaactcaccaacgg caaattattggcggccatccgtgagtcgctcgatgccgcgcttatccagctcaacgggccaatcaatatgctggcggctgcgcgtcgtcttaacggcgaagaaggcaacaacatggcgctggaattcgcctggcgcgaaggcgagcaggcggtgagtcgcttacaggcctgccgtccgtcgctggatttga gccgcaggcagaatggccggtcagtgaattctttgacgatctgagcgcgctgtacgacagccattttctcagtgacggtgaattgcgttacgtggtcatgccatctgatctgcacgctgtcgggcaacgaacgcaaatccttaccgcgctgagcttatggattgatcacacgctgtcacaggcgcaggccatgcc gtctctgaagctctcggtgaacattgttgcgaggcaggatgcgagctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgcgttatgcccgcccggaagcggcgttttcccgtccggggaatggcatggagctgcgccttatccagacgctgatcgcccatcatcgcggttctttagatctctcggtcc gccctgatggcggcaccttgctgacgttacgcctgccggtacagcaggttatcaccgggaggcttaaaatga 74 atgacccagttacctaccgcgggcccggttatccggcgctttgatatgtctgcccagtttacggcgctttatcgcatcagcgtggcgctgagtcaggaaagcaacaccgggcgcgcactggcggcgatcctcgaagtgcttcacgatcatgcatttatgcaatacggcatggtgtgtctgtttgataaagaacg caatgcactctttgtggaatccctgcatggcatcgacggcgaaaggaaaaagagacccgccatgtccgttaccgcatgggggaaggcgtgatcggcgcggtgatgagccagcgtcaggcgctggtgttaccgcgcgcatttcagacgatcagcgttttctcgaccgcctgaatatttacgattacagcctgccgttga ttggcgtgccgatccccggtgcggataatcagccatcgggcgtgctggtggcacagccgatggcgttgcacgaagaccggctgactgccagtacgcggtttttagaaatggtcgccaatctcatcagccagccactgcgttctgccacgcccccggaatcattgcctgctcaaacgccggtccggtgcagtgttccgcg ccagtttggtttcgagcagatggtcgggaaaagtcaggcgatgcgccagacgatggacattttacggcaggtttccaaatgggataccacggttctggtgcgtggtgaaagcggcaccggcaaggaacttatcgccaatgccattcattacaactcaccccgtgcggccgcgccatttgtgaaattcaactgcgccgcgctgcc ggataacctgctggaaagcgaactgttcggtcatgaaaaaagcgaactgttcggtcatgaaaaaggggccttcaccggcgctatccgtacccgtaaaggccgctttgaactggcggacgggggcacgttattcctcgatgaaatcggcgaatcgagcgcgtcgtttcaggccaaattgctgcgcattttgcaggaaggtgaaatggaacgggtcggcgg cgataccacgctgaaagttgatgtgcgcattattgctgccaccaaccgtaatcttgaagaggaagtgcgtgccgggaattttcgcgaagacctgtattatcgcctgaacgtgatgccggttcgctgcctgcactgcgtgaaaaggctggatgatatcgccgatctggcgccgtttctggtcaaaaagattgc gctgcgtcaggggcgggaactgcgcatcagcgacggtgcggtgcgtctgctgatgacctacagctggccaggcaacgtgcgtgaactggaaaactgtctcgaacgggcgtcggtaatgaccgatgaagggctgatcgaccgcgacgtgatcctgttcaatcaccatgaatccccggcgctgtccgtcaaaccc ggcctgccgctcgcgacagatgaaagctggctggatcaggaactcgacgaacgccagcgggtgattgccgcactggagaaaaccggctgggtgcaggccaaagcggcccgactgctgggcatgacaccgcgccagattgcctaccgtatccagattatggacatcaacatgcaccgtatctga 75 aaaactaccgccgcaattaatgaacccaacgctactgttgccgggccatgctcttccccggcgcgctgcccgggaaaggatatagattgcccagcacgcgccagcaccaagcgcgaacgccgcgccagtgagatcaacatgtgaaacattttcgcccagcggcagcagatacaagaggccaagtaccgccaggatcacccagatgaa atccaccgggcggcgtgaggcaaaaagcgccaccgccagcgggccggtaaattccagcgccaccgcaacgccgagcggtatcgtctggatcgataaatagaacatatagttcatggcgccgagcgacaggccataaaacagcagtggcaggcgttgttcacgggtaaaatgtaaacgccagggcttgaacactacgaccaaaagatagatgg tgccaagtgcgagacgcagcgcggtgacgccgggtgcgccaacaatcggaaacagtgatttcgccagcgacgcgcctccctgaatggacatcgcgacaaacaatattaataccggcaaccacaccggcaatttacgagactgcgcaggcatccttctcccgtcaatttctgtcaaataaagtaaaagaggcagtactact tgaattacccccggctggttgagcgtttgttgaaaaaaagtaactgaaaaatccgtagaatagcgccactctgatggttaattaattaacctattcaattaagaattatctggatgaatgtgccattaaatgcgcagcataatggtgcgttgtgcgggaaaactgcttttttttgaaagggttggtcagtagcggaaactttctgttacatcaaat ggcgctttgaccccaattcccgcaaagagtttcttaactaattttgatatatttaaacgcgtaggacgtaggatttacttgaagcacatttgaggtggattatgaaaaaaattgcatgtctttcagcactggccgcacttctggcggtttctgcaggttccgcagtagcagcaacttcaaccgtaactggcggctacgctcagagcgacgct cagggtattgctaacaaaactaacggtttcaacctgaaatatcgctacgagcaggacaacaacccgctgggtgttatcggttcctttacttacactgaaaaagatcgcaccgaaagcagcgtttataacaaagcgcagtactacggcatcaccgcaggcccggcttaccgcatcaacgactgggcgagcatctacggtgttgtggg tgtaggttacggtaaattccagcagactgtagacaccgctaaagtgtctgacaccagcgactacg 76 aacacacgctcctgttgaaaaagagatcccgccgggaaatgcggtgaacgtgtctgatattgcgaagagtgtgccagttttggtcgcgggcaaaacctgcaccagtttggttattaatgcaccagtctggcgctttttttcgccgagtttctcctcgctaatgccgccaggcgcggctttggcgctgatagcg cgctgaataccgatctggatcaaggttttgtcgggttatcagccaaaaggtgcactctttgcatggttatacgtgcctgacatgttgtccgggcgacaaacggcctggtggcacaaattgtcagaactacgacacgactaactgaccgcaggagtgtgcgatgaccctgaatatgatgatggatgccggcgcgcccgaggcaat cgccggtgcgctttcgcgacaccatcctgggctgttttttaccatcgttgaagaagcgcccgtcgccatttcgctgactgatgccgacgcacgcattgtctatgccaacccggctttctgccgccagaccggctatgaactagaagcgttgttgcagcaaaatccccgcctgcttgcaagtcgccaaaccccacgggaaat ctatcaggatatgtggcacaccttgttacaacgccgaccgtggcgcgggcaattgattaaccgccaccgcgacggcagcctgtatctggtcgagatcgatatcaccccggtgattaacccgtttggcgaactggaacactacctggcaatgcagcgcgatatcagcgccagttatgcgctggagcagcggttgcgcaatcacat gacgctgaccgaagcggtgctgaataacattccggcggcggtggttgtagtggatgaacgcgatcatgtggttatggataaccttgcctacaaaacgttctgtgccgactgcggcggaaaagagctcctgagcgaactcaatttttcagcccgaaaagcggagctggcaaacggccaggtcttaccggtggtgctgcgcggtga ggtgcgctggttgtcggtgacctgctgggcgctgccgggcgtcagcgaagaagccagtcgctactttattgataacaggctgacgcgcacgctggtggtgatcaccgacgacacccaacaacgccagcagcaggaacagggccgacttgaccgccttaaacagcagatgaccaacggcaaactactggcagcgatccgcga agcgcttgacgccgcgctgatccagcttaactgccccatcaatatgctggcggcggcgcgacgtttaaacggcagtgataacaacaatgtggcgctcgacgccgcgtggcgcgaaggtgaagaggcgatggcgcggctgaaacgttgccgcccgtcgctggaactggaaagtgcggccgtctggccg ctgcaacccttttttgacgatctgcgcgcgctttatcacacccgctacgagcaggggaaaaatttgcaggtcacgctggattcccatcatctggtgggatttggtcagcgtacgcaactgttagcctgcctgagtctgtggctcgatcgcacgctggatattgccgccgggctgggtgatttcaccgcgcaaacg cagatttacgcccgcgaagaagagggctggctctctttgtatatcactgacaatgtgccgctgatcccgctgcgccacacccactcgccggatgcgcttaacgctccgggaaaaggcatggagctgcgcctgatccagacgctggtggcacaccaccacggcgcaatagaactcacttcacaccccgaagggggaagttgcctgaccctacga . cagcacggcatgatctgtctgtacgacgccagcaggcgattttgaatattgaagcgttgcaggaagccgatcagcagttaatccccggcagctcgcaaatccgctatcgtccgggcgaagggctggtcgggacggtgctttcgcagggccaatcattagtgctggcgcgcgttgctgacgatcagcgctttcttgaccgg ctcgggttgtatgattacaacctgccgtttatcgccgtgccgctgatagggccagatgcgcagactttcggtgtgctgacggcacaacccatggcgcgttacgaagagcgattacccgcctgcacccgctttctggaaacggtcgctaacctggtcgcgcaaaccgtcgcgtttgatggcaccaccggcagtgc gcccttccccgcgcgccgccataacacaggccgccagcccgaaatcctgcacggcctcacgcgcatttggttttgaaaatatggtcggtaacagtccggcgatgcgccagaccatggagattatccgtcaggtttcgcgctgggacaccaccgttctggtacgcggcgagtggcaccggcaaggagctgattgccaacgccat ccaccaccattcgccgcgtgccggtgcgccatttgtgaaattcaactgtgcggcgctgccggacacactgctggaaagcgaattgttcggtcacgagaaaggggcatttaccggcgcggtacgccagcgtaaaggccgttttgagctggccgatggcggcacgctgtttcttgacgagatcggcgagagtagcg cctcgtttcaggctaagctgctgcgcattttgcaggaaggcgaaatggaacgcgtcggcggcgacgagacattgcaagtgaatgtgcgcattattgccgcgacgaaccgcaatcttgaagatgaagtccggctggggcactttcgcgaagatctctattatcgcctgaatgtgcccatcgccctgccgccactac gcgaacgccaggaggacattgccgagctggcgcactttctggtgcgtaaaatcgcccataaccagagccgtacgctgcgcattagcgagggcgctatccgcctgctgatgagctacaactggcccggtaatgtgcgcgaactggaaaactgccttgagcgctcagcggtgatgtcggagaacggtctgatcgatc gggatgtgattttgtttaatcatcgcgaccagccagccaaaccgccagttatcagcgtctcgcatgatgataactggctcgataacaaccttgacgagcgccagcggctgattgcggcgctggaaaaagcgggatgggtacaagccaaagccgcgcgcttgctggggatgacgccgcgccaggtcgcctatcgtatt cagacgatggatataaccctgccaaggctataa 77 MTLNMMMDAGAPEAIAGALSRHHPGLFFTIVEEAPVAISLTDADARIVYANPAFCRQTGYELEALLQQNPRLLASRQTPREIYQDMWHTLLQRRPWRGQLINRHRDGSLYLVEIDITPVINPFGELEHYLAMQRDISASYALEQRLRNHMTLTEAAVLNNIPAAVVVVDERDHVVMDNLAYKTFCADCGGKELLSELNFSARKAELANGQ VLPVVLRGEVRWLSVTCWALPGVSEEASRYFIDNRLTRTLVVITDDTQQRQQQEQGRLDRLKQQMTNGKLLAAIRREALDAALIQLNCPINMLAAARRLNGSDNNNVALDAAWREGEEAMARLKRCRPSLELESAAVWPLQPFFDDLRALYHTRYEQGKNLQVTLDSHHLVGFGQRTQLLACLSLWLDRTLDIAAGLGDFTAQTQ IYAREEEGWLSLYITDNVPLIPLRHTHSPDALNAPGKGMELRLIQTLVAHHHGAIELTSHPEGGSCLTLRFPLFHSLTGGSK 78 MTQRTESGNTVWRFDLSQQFTAMQRISVVLSRATEVDQTLQQVLCVLHNDAFLQHGMICLYDSQQAILNIEALQEADQQLIPGSSQIRYRPGEGLVGTVLSQGQSLVLARVADDQRFLDRLGLYDYNLPFIAVPLIGPDAQTFGVLTAQPMARYEERLPACTRFLETVANLVAQTVRLMAPPAVRPSPRAAITQ AASPKSCTASRAFGFENMVGNSPAMRQTMEIIRQVSRWDTTVLVRGESGTGKELIANAIHHHSPRAGAPFVKFNCAALPDTLLESELFGHEKGAFTGAVRQRKGRFELADGGTLFLDEIGESSASFQAKLLRILQEMEMERVGGDETLQVNVRIIAATNRNLEDEVRLGHFREDLYYRLNVMPIALPPLRERQEDIAELAHFLVRKIAHNQSRTL RISEGAIRLLMSYNWPGNVRELENCLERSAVMSENGLIDRDVILFNHRDQPAKPPVISVSHDDNWLDNNLDERQRLIAALEKAGWVQAKAARLLGMTPRQVAYRIQTMDITLPRL 79 atgccgcaccacgcaggattgtcgcagcactggcaaacggtattttctcgtctgccggaatcgctcaccgcgcagccattgagcgcgcaggcgcagtcagtgctcacttttagtgattttgttcaggacagcatcatcgcgcatcctgagtggctggcagagcttgaaagcgcgccgccgcctgcgaacgaatgg caacactatgcgcaatggctgcaagcggcgctggatggcgtcaccgatgaagcctcgctgatgcgcgcgctgcggctgtttcgccgtcgcatcatggtgcgcatcgcctggagccaggcgttacagttggtggcggaagaagaagatatcctgcaacagcttagcgtgctggcggaaaccctgatcgtcgccgc gcgcgactggctttatgaggcctgctgccgtgagcctgctgccgtgagtggggaacgccgagcaatccacaaggcgtggcgcagccgatgctggtactcggcatgggcaaactgggtggcggcgaactcaatttctcatccgatatcgatttgattttcgcctggccggaaaatggcgcaacgcgcggtggacgccgtgagctggataacgcg caatttttcactcgccttggtcaacggctgattaaagtcctcgaccagccaacgcaggatggctttgtctaccgcgcttgcgcccgttggcgacagcggcccgctggtgctgagctttgccgcgctggaagattactaccaggagcaggggcgcgattgggaacgctacgcgatggtgaaagcgcg cattatgggcgataacgacggcgaccatgcgcgggagttgcgcgggagttgcgcgcaatgctgcgccgtttgttttccgccgttatcgacttcagcgtgattcagtccctgcgtaacatgaaaggcatgattgcccgcgaagtgcgcgccgtggcctgaaggacaacattaagctcggcgcgggcgggatccgcga aatagaatttatcgtccaggttttccagctgattcgcggcggtcgcgagcctgcactgcaatcgcgttcactgttgccgacgcttgctgccatagatcaactgcatctgctgccggatggcgacgcaacccggctgcgcgaggcgtatttgtggctgcgacggctggagaacctgctgcaaagcatcaatgacgaacagacacag . atgcgttgcaggaggaggattccacgcccgtgctggcgcatctctcagaggacgatcgccgccgcgtggtggcgctgattgccgattttcgcaaagagttggataaacgcaccattggcccgcgagggcggcaggtactcgatcacttaatgccgcatctgctcagcgatgtatgctcgcgcgacgatgcgccag taccgctgtcacgcctgacgccgctgctcaccggaattattacccgcaccacttaccttgagctgctaagtgaatttcccggcgcactgaaacacctcatttccctgtgtgccgcgcgccgccgatggttgccagtcagctggcgcgctacccgatcctgcttgatgaattgctcgacccgaatacgctctatcaaccgac ggcgatgaatgcctatcgcgatgagctgcgccaatacctgctgcgcgtgccggaagatgatgaagagcaacagcttgaggcgctgcggcagtttaagcaggcgcagttgctgcgcgtggcggcggcggatattgccggtacgttgccagtaatgaaagtgagcgatcacttaacctggctggcggaagcgattatt gatgcggtggtgcagcaagcctggggggcagatggtggcgcgttatggccagccaacgcatctgcacgatcgcgaagggcgcggttttgcggtggtcggttatggcaagctgggcggctgggagctgggttacagctccgatctggatctggtattcctgcacgactgcccgatggatgtgatgaccgatggcgagcgt gaaatcgatggtcgccagttctatttgcgtctcgcgcagcgcgtgatgcacctgtttagcacgcgcacgtcgtccggcatcctttatgaagttgatgcgcgtctgcgtccatctggcgctgcggggatgctggtcactactacggaatcgttcgccgattaccagcaaaacgaagcctggacgtggga acatcaggcgctggccgtgcgcgcgtggtgtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatattctgatgacgcctcgcgacggcgcaacgctgcaaaccgacgtgcgagaaatgcgcgagaaaatgcgtgcccatcttggcaacaagcataaagaccgcttcgatct gaaagccgatgaaggcggtatcaccgacatcgagtttatcgcccaatatctggtgctgcgctttgcccatgacaagccgaaactgacgcgctggtcggataatgtgcgcattctcgaagggctggcgcaaaacggcatcatggaggagcaggaagcgcaggcattgacgctggcgtacaccacattgcgtgatgagctgcaccacc tggcgctgcaagagttgccgggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttattaaaaccagctgggacaagtggctggtggaaccgtgcgccccggcgtaa 80 atgtttaacgatctgattggcgatgatgaaacggattcgccggaagatgcgctttctgagagctggcgcgaattgtggcaggatgcgttgcaggaggaggattccacgcccgtgctggcgcatctctcagaggacgatcgccgccgcgtggtggcgctgattgccgattttcgcaaagagttggataaacgcaccatt ggccgcgagggcggcaggtactcgatcacttaatgccgcatctgctcagcgatgtatgctcgcgcgcgacgatgcgccagtaccgctgtcacgcctgacgccgctgctcaccggaattattacccgcaccacttaccttgagctgctaagtgaatttcccggcgcactgaaacacctcatttccctgtgtgccgcgt cgccgatggttgccagtcagctggcgcgctacccgatcctgcttgaatgaattgctcgacccgaatacgctctatcaaccgacggcgatgaatgcctatcgcgatgagctgcgccaatacctgctgcgcgtgccggaagatgatgaagagcaacagcttgaggcgctgcggcagtttaagcaggcgcagttgct gcgcgtggcggcggcggatattgccggtacgttgccagtaatgaaagtgagcgatcacttaacctggctggcggaagcgattattgatgcggtggtgcagcaagcctggggggcagatggtggcgcgttatggccagccaacgcatctgcacgatcgcgcgaagggcgcggttttgcggtggtcggttatggcaagctggg cggctgggagctgggttacagctccgatctggatctggtattcctgcacgactgcccgatggatgtgatgaccgatggcgagcgtgaaatcgatggtcgccagttctatttgcgtctcgcgcagcgcgtgatgcacctgtttagcacgcgcacgtcgtccggcatcctttatgaagttgatgcgcgt ctgcgtccatctggcgctgcggggatgctggtcactactacggaatcgttcgccgattaccagcaaaaacgaagcctggacgtgggaacatcaggcgctggcccgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatattctgatgacgcctcgcgacggcgcaac gctgcaaaccgacgtgcgagaaatgcgcgagaaaatgcgtgcccatcttggcaacaagcataaagaccgcttcgatctgaaagccgatgaaggcggtatcaccgacatcgagtttatcgcccaatatctggtgctgcgctttgcccatgacaagccgaaactgacgcgctggtcggataatgtgcgcattctcga agggctggcgcaaaacggcatcatggaggcaggaagcgcaggcattgacgctggcgtacaccacattgcgtgatgagctgcaccacctggcgctgcaagagttgccgggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttattaaaaccagctgggacaagtggctggtggaaccgtgcgccccggc gtaa 81 MPHHAGLSQHWQTVFSRLPESLTAQPLSAQAQSVLTFSDFVQDSIIAHPEWLAELESAPPPANEWQHYAQWLQAALDGVTDEASLMRALRLFRRRIMVRIAWSQALQLVAEEDILQQLSVLAETLIVAARDWLYEACCREWGTPSNPQGVAQPMLVLGMGKLGGGELNFSSDIDLIFAWPENGATRGGRRELDNAQFFTRLGQ RLIKVLDQPTQDGFVYRVDMRLRPFGDSGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDNDGDHARELRAMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLKDNIKLGAGGIREIEFIVQVFQLIRGGREPALQSRSLLPTLAAIDQLHLLPDGDATRLREAYLWLRRLENLLQSINDEQTQTLPGDELNRARLAWG MGKDSWEALCETLEAHMSAVRQIFNDLIGDDETDSPEDALSESWRELWQDALQEEDSTPVLAHLSEDDRRRVVALIADFRKELDKRTIGPRGRQVLDHLMPHLLSDVCSRDDAPVPLSRLTPLLTGIITRTTYLELLSEFPGALKHLISLCAASPMVASQLARYPILLDELLDPNTLYQPTAMNAYRDELRQYLLRVPEDDEEQQLEALRQFKQ AQLLRVAAADIAGTLPVMKVSDHLTWLAEAIIDAVVQQAWGQMVARYGQPTHLHDREGRGFAVVGYGKLGGWELGYSSDLDLVFLHDCPMDVMTDGEREIDGRQFYLRLAQRVMHLFSTRTSSGILYEVDARLRPSGAAGMLVTTTESFADYQQNEAWTWEHQALARARVVYGDPQLTAEFDAIRMTPRDGATLQTDVREM REKMRAHLGNKHKDRFDLKADEGGITDIEFIAQYLVLRFAHDKPKLTRWSDNVRILEGLAQNGIMEEQEAQALTLAYTTLRDELHHLALQELPGHVALSCFVAERALIKTSWDKWLVEPCAPA 82 MFNDLIGDDETDSPEDALSESWRELWQDALQEEDSTPVLAHLSEDDRRRVVALIADFRKELDKRTIGPRGRQVLDHLMPHLLSDVCSRDDAPVPLSRLTPLLTGIITRTTYLELLSEFPGALKHLISLCAASPMVASQLARYPILLDELLDPNTLYQPTAMNAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQLLRVAAADIAGTLPVMK VSDHLTWLAEAIIDAVVQQAWGQMVARYGQPTHLHDREGRGFAVVGYGKLGGWELGYSSDLDLVFLHDCPMDVMTDGEREIDGRQFYLRLAQRVMHLFSTRTSSGILYEVDARLRPSGAAGMLVTTTESFADYQQNEAWTWEHQALARARVVYGDPQLTAEFDAIRRDILMTPRDGATLQTDVREMREKMRAHLGNKHKDRF DLKADEGGITDIEFIAQYLVLRFAHDKPKLTRWSDNVRILEGLAQNGIMEEQEAQALTLAYTTLRDELHHLALQELPGHVALSCFVAERALIKTSWDKWLVEPCAPA 83 EEQQLEALRQFKQAQLLRVAAADIAGTLPVMKVSDHLTWLAEAIIDAVVQQAWGQMVARYGQPTHLHDREGRGFAVVGYGKLGGWELGYSSDLDLVFLHDCPMDVMTDGEREIDGRQFYLRLAQRVMHLFSTRTSSGILYEVDARLRPSGAAGMLVTTTESFADYQQNEAWTWEHQALARARVVYGDPQLTAEFD AIRRDILMTPRDGATLQTDVREMREKMRAHLGNKHKDRFDLKADEGGITDIEFIAQYLVLRFAHDKPKLTRWSDNVRILEGLAQNGIMEEQEAQALTLAYTTLRDELHHLALQELPGHVALSCFVAERALIKTSWDKWLVEPCAPA 84 ccgagcgtcggggtgcctaatatcagcaccggatacgagagaaaagtgtctacatcggttcggttgatattgaccggcgcatccgccagcccgcccagtttctggtggatctgttggcgattttgcgggtcttgccggtgtcggtgccgaaaaaaataccaatatttgccataacacacgctcctgttgaaaaaga gatcccgccgggaaatgcggtgaacgtgtctgatattgcgaagagtgtgccagttttggtcgcgggcaaaacctgcaccagtttggttattaatgcaccagtctggcgctttttttcgccgagtttctcctcgctaatgcccgccaggcgcggctttggcgctgatagcgcgctgaataccgatctggatcaaggtttt gtcgggttatcagccaaaaggtgcactctttgcatggttatacgtgcctgacatgttgtccgggcgacaaacggcctggtggcacaaattgtcagaactacgacacgactaactgaccgcaggagtgtgcgatgaccctgaatatgatgatggatgccggcggacatcatcgcgacaaacaatattaataccggcaaccaccggcaat ttacgagactgcgcaggcatcctttctcccgtcaatttctgtcaaataaagtaaaagaggcagtctacttgaattacccccggctggttgagcgtttgttgaaaaaaagtaactgaaaaatccgtagaatagcgccactctgatggttaattaacctattcaattaagaattatctggatgaatgtgccattaaatgcgcagcataatggtgcg ttgtgcgggaaaactgcttttttttgaaagggttggtcagtagcggaaacaactcacttcacaccccgaagggggaagttgcctgaccctacgattcccgctatttcattcactgaccggaggttcaaaatgacccagcgaaccgagtcgggtaataccgtctggcgcttcgatttgtcccagcagttcactgcgatgcagc gcataagcgtggtactcagccgggcgaccgaggtcgatcagacgctccagcaagtgctgtgcgtattgcacaatgacgcctttttgcagcacggcatgatctgtctgtacgacgccagcaggcgattttgaatattgaagcgttgcaggaagccgatcagcagttaatccccggcagctcgcaaatccgctatcgtcc gggcgaagggctggtcgggacggtgctttcgcagggccaatcattagtgctggcgcgcgttgctgacgatcagcgctttcttgaccggctcgggttgtatgattacaacctgccgtttatc 85 attgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgagcggcagcgggaagtagcttgctactttgccggcgagcggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataccgcatgacctcgnaagagcaaagtgggggatcttcggacct cacgccatcggatgtgcccagatgggattagctagtaggtgaggtaatggctcacctaggcgacgatccctagctggtctgaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtgtgaagaaggccttagggttg taaagcactttcagcgaggaggaaggcatcanacttaatacgtgtggtgattgacgttactcgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggtttgttaagtcagatgtgaaatccccgcgcttaacgtgggaactg catttgaaactggcaagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgctgtaaacgatgtcgacttggaggttgtgcccttga ggcgtggcttccggagctaacgcgttaagtcgaccgcctggggagtacggccgcaaggttaaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctactcttgacatccacggaattcgccagagatggcttagtgccttcgggaaccgtganacaggt gctgcatggctgtcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatcctttgttgccagcnngtnatgnngggaactcaaaggagactgccggtgataaaccggaggaaggtggggatgacgtcaagtcatcatggcccttacgagtagggctacacacgtgctacaatggcatatacaa agagaagcgaactcgcgagg 86 atggcaatgcgtcaatgcgcaatctacgggaaagggggtattgggaaatccaccactacccaaaaccttgtagcggctctctggccgaaatgaataagaaggtcatgatcgtcggctgtgaccctaaggctgattcaacccgcctcattctgcatgcgaaagcacagaacaccatcatggaaatggccgctgaagtgggctccgtggaagatctgg agctggaagatgtgatgcaaatcggctatggcggcgtgcgctgtgcggaatcaggcggccctgagcctggtgtgggttgtgccggacgcggggtgatcaccgccatcaacttcctcgaagaagaaggcgcgtatgtgccggatctggattttgtgttttacgacgtattgggcgatgtggtctgtggc ggtttcgcgatgccaattcgcgaaaacaaagcgcaggaaatctacatcgtgtgctccggtgaaatgatggcgatgtatgccgccaacaacatttccaaaggcatcgtgaaatacgcgaaatcgggcaaagttcgcctggccgggctgatctgtaactccgccagacggatcgcgaagatgaactgatcatcg cgctggctgaaaaacttggcacgcaaatgatccacttcgtgccgcgtgacaacattgtgcaacgcgctgaaatccgccgcatgacggtcatcgaatacgacccgacttgtgcgcaggcagatcagtatcgtgcactggcgaacaaaatcgtcaacaacaccaaaatggtggtgccgacaccggtcaccat ggatgagctggaagccctgttaatggaatttggcattatggaagaagaagacctggccatcgtcggtcgtaccgccgccgaagaggcgtga 87 atgaaggcaaaagagattctggcgctgattgatgagccagcctgtgagcataaccacaagcagaagtcgggttgcagcctgccgaaaccgggcgcgacggcaggcggttgtgcgttgatggcgcgcagattgcgctgctgccggtcgcggacgtcgcgcatctggtgcacggcccgattggctgtacc ggcagttcatgggacaaccgtggcagccgcagttccggggccttccatcaaccgcatgggcttcaccaccgacatgagcgagcaggatgtgattatggggcgcggcgagcgacgcttatttcacgccgtgcagcacatcgtcagccattaccatccggtggcggtctttatttacaacacctgcgtacccgcgatggaaggggat gacgttgaagccgtgtgtcgcgccgcatcggccgctgccggtgtgccggttatttcagtcgatgccgccggtttctacggcagcaaaaatctcggtaaccgcattgccggggacgtgatggtcaaaaaggtgatcggccagcgcgaacccgcgccgtggccggaaaactcaccgatccccgccggacac cgccacagcatcagcctgattggcgaattcaatattgccggcgagttctggcacgttctgccgctgctcgatgagctcgggatccgcgtgctgtgcagcctttccggggattcccgttttgctgaaatccagactatgcaccgtggcgaagccaacatgctggtgtgctcgcgggcgctgatcaacgtcgcccga aaaatggaagagcgttaccagatcccatggtttgaaggcagtttttatggcctgcgttccatggctgattccctgcgcacgatcgccgtgctgctcaaagacccggatttacaggcgcgcacagaacgtctgattgagcgcgaggaggcggcgacacatcttgcgcttgcgccttaccgtgcgcggctcag cgggcgcaaggcgctgctgtataccggtggcgtgaaatcctggtcggtggtcggcgttacaggatttaggcatcacggtggtggcgaccggcacccgaaaatcaaccgaagaagaagaagcagcgtattcgcgaactgatgggtgaagacgtgctgatgctcgacgaaggcaatgccagaaccttgctcgacaccctct atcgtttcggcggcgacatcatgatcgccgggggccgcaacatgtataccgcgtacaaagcccgcctgccgttcctggatatcaatcaggagcgcgagcatgcgtttgccggatatcacgggctggtaaatctggccgaacagttgtgtatcaccctggaaagcccggtctgggcgcaggtcaaccgtctggcgccg tggcgctaa 88 atgaccagtgaaacacgcgaacgtaacgaggcattgatccaggaagtgctggagatcttccccgagaaggcgcttaaagatcgtaagaaacacatgatgaccaccgacccggcgatggaatctgtcggcaagtgtattgtctcaaaccgcaaatcacagccgggcgtgatgaccgtgcgaggctgcgcttacgccggtt ccaaaggcgtggtctttggcccgatcaaagacatggcgcatatctcccacggcccggttggttgcggccagtattcccgtgccggacgccgtaactattacaccggctggagcggcgtgaacagctttggcaccctcaacttcaccagtgattttcaggaacgggacatcgtatttggcggcgataaaaagctcgacaaattgatcgatgaact ggagatgttgttcccgctgagcaaaggcatttcggtgcagtcggaatgtccggtcggtctgatcggcgatgacatttctgccgtcgccaaagccagcagcgccaaaatcggtaagccggtcgtgccggtacgctgcgaggggttccgcggtgtgtcgcaatcgctcggccatcacattgctaacgatgt catccgcgactgggtgctggataaccgcgaaggcaatgaatttgaaaccacgccttacgacgtggcgattatcggcgactacaacatcggcggtgacgcctgggcctcacgtattctgctcgaagaaatggggctgcgcgtggtggcgcagtggtccggcgacggcacgctggtggagatggaaaacaccccgaa agtcgcgctcaatctggtgcactgctaccgctcgatgaactacatctcccgtcatatggaagaaaaacacggcattccgtggatggaatacaacttctttggccgaccaaaattgcggaatctctgcgcgaaatcgcggcgcgttttgacgataccatccggaaaaacgccgaagcggtgattgaaaaatatatatcaggc gcaaacgcaggcggtgatcgacaaataccgtccgcgtctggaaggcaaaaaggtgctgttgtatctcggcggtttacgtccgcgccacatcatcggggcgtatgaagatctgggaatggaaatcatcggtaccggctatgaattcggtcataacgatgattacgaccgcaccttaccgatgctcaaagaaggcacgttg ctgttcgatgacctgagcagttatgagctggaagcgttcgttaaagcgctgaaaccggatcttgtcgggtcaggtatcaaagaaaaatacattttccagaaaatgggcgtgccgttccgccagatgcactcctgggattattccggcccttatcacggctacgacggttcggcatttttgcccgtgacatggacatgacg ctgaacaatccgggctggagtcagctgaccgccccctggttgaaaacggcctga 89 atggctcaaattctgcgtaatgccaagccgcttgccaccacgcctgtcaaaagcgggcaaccgctcggggcgatcctggccagtcaggggctggaaaattgcatcccgctggttcacggcgcgcaaggttgtagcgcgttcgccaaagttttcttcatccagcattttcacgatccgatcccgttgcagtccacgg cgatggaatcgaccacgactatcatgggctcggatggcaacgtcagtactgcgttgaccacgttgtgtcagcgcagtaatccaaaagccattgtgattttgagcaccggactgtcagaagcgcagggcagtgatttgtcgatggcgctgcgtgagtttcgcgacaaagaaccgcgctttaatgccatcgctat tctgaccgttaacacgccggatttttacggctcgctggaaaacggctacagcgcgctgatggaaagcgtgatcactcagtgggtgccggaaaagccgccgaccggcatgcgtaacaagcgcgtgaacctgctggtgagccatctgctgacgccgggggatctggaattactgcgcagctatgtcgaagcctttgg cctgcaaccggtgatcctgccggatttatcacagtcgctggacggacatctggcgaatggcgatttcaatccggtcacgcagggcggcacgtcgcaacgccagattgaacaaatggggcagagcctgaccaccattaccattggcagttcgctcaactgcgccgccagtctgatggcgatgcgcagccgtggcatggcgctgaac ctgccgcacctgatgacgctggaaaacatggacagtctgatccgccatctgcatcaggtgtcaggccgcgaggtaccggcatggattgagcgccagcgcgggcaactgctggacgccatgatcgactgccatacctggctgcagtcacagcgtattgcgctggcggcagaagcggatttgctggtggcgtggtgcgat ttcgctcagagccagggaatgcgcgtcggggccggtgattgcgccggttaatcagcagtcactggccgggctgccggtcgaacaggtggtgatcggcgatctggaagatttacaaacccggctcgacagctacccggtttcactgctggtggcgaactcccacgctgcaccactggcggaaaaaaacggtatcgcgct ggtacgtgccggtttcccgctttacgaccgtctcggggaatttcgccgcgtgcggcagggctatgcgggtattcgcgacaccttgttcgaactcgcgaacctgatgcaggcgcgccatcacatgctgacggcgtatcactcaccgcttaggcaggtgttcggcctgagcccggtaccggaggccagtcatgaggcg cgctaa 90 atgagtcaagatcttggcaccccaaaatcctgtttcccgctgttcgagcaggatgaataccagaatatgtttacccacaaacgcgcgctggaagaagcacacggcgaggcgaaagtgcgggaagtgtttgaatggaccaccacgcaggaatatcaggatctgaacttctcgcgtgaagcgctgaccgtcgacccggcgaaag cctgccagccgttaggcgcggtactttgcgcgctgggttttaccaacacgttgccgtatgtccatggttcacaaggctgtgtggcgtatttccgtacctattttaatcgtcatttcaaagagccggtggcctgtgtttccgactcaatgaccgaagaatgccgccgttttggcggaaataacaacatgaatgtcggt ctggaaaacgccagcgcgctgtacaagccggaaattattgcggtctccaccacctgtatggcggaagtgatcggtgatgacctgcaggctttttcgccaacgccaaaaaagacggatttgtggatgccggtatgccaatcccgtatgcccatacaccgagttttctgggcagtcatgtcaccggctgggacaacatgtttga aggcttcgcccgtacctttaccaccgacgccacgcgggaatatcagccgggcaaacttgccaaactgaacgtggtgaccggttttgaaacttatctcggcaactaccgggttattcaccgcatgatgagccagatgggggtcgaatgcagcgtcttgtccgatccgtctgaagtgctcgacaccccggctgacggccaataccgcatg tatgccggcggcaccacgcaaaccgaaatgcgtgatgcaccggatgccatcgacaccttgctgctgctgcaaccgtggcaattacagaaaaccaaaaaggtggtgcagggcgactggaatcagccgggcaccgaagtcagtgtaccgattggcctggcggcgaccgatgccttgctgatgacggtaagcgaactgaccggcaa accgatagctgacgcgctggcgactgaacgtggccgtctggtggacatgatgctcgattctcacacctggctgcacggcaagcgtttcggtctctacggtgacccggattttgtgatgggcatgaccgcattcctgctggaactgggctgtgaaccgaccaccattctcagccataacggcaacaaacgctggcagaaagccatgaagaa aatgctggctgattcgccttacggacaggacagcgaagtgtatgtgaactgcgatctgtggcatttccgctcgctgatgtttacccgtaaaccggactttatgatcggcaactcttacggaaaattcattcagcgtgacacgctggccaaaggcgaacagttcgaagtgccgctgatccgtatcggattcccgattttt gaccggcaccatttgcaccgtcagaccacctggggatacgagggcgcgatgagcatcctgacgcaactggtgaatgcggtgctcgaacagctggatcgcgaaaccatgaagctcggcaaaaccgactacaacttcgatctgatccgctaa 91 atgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggttgaacaatcatcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaacca ccgtctgctggccagccagcagacgccgaaacatatctatgacgaaatgtggcgcactttgttgcagggcaaatcctggaacggccaactgatcaaccggcgtaataaccgttcgctttatctggcggatgtcactatcacgcctgttttaggcgcggacgggcaggtggagcattacctcggcatgcacaaagatatcagcgaga aatacgcgctggagcagcggttgcgcaaccacatcaccttgttcacggaggtgctgaacaatattcccgccgccgtggtggtggtggatgagcaggacaatgtggtgatggacaatctggcctacaaaaccctgtgcgctgactgcggcggaaaagagctgttggccgaaatgggctatccgcaactcaaagagatgct caacagtggcgaaccggtgccggtttccatgcgcggttttctttcggtcagtggtcattgcagggcgttaatgaagaggccagccgcttttttaccggcattaccgcgccgggaaaactgattgttctcaccgactgcaccgatcagcatcaccggcagcagcagggttatcttgaccggctcaagcaaaaacttacca acggcaaattgctggcagccatccgcgagtcgcttgatgccgcgctgattcagctcaacgggccaattaatatgctggcggctgcgcgtcgtcttaacggcgaagaaggcaacaacatggcgctggaattcgcctggcgcgaaggcgagcaggcggtgagtcgcttacaggcctgccgtccgtcgctggat tttgagccgcaggcagaatggccggtcagtgaattcttcgacgatctgagcgcgctgtacgacagccattttctcagtgacggtgaattgcgttacgtggtcatgccatctgatctgcacgctgtcgggcaacgaacgcaaatccttaccgcgctgagcttatggattgatcacacgctgtcacaggcgcagg ccatgccgtctctgaagctctcggtgaacattgttgcgaagcaggatgcgagctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgcgttatgcccgcccggaagcggcgttttcccgtccggggaatggcatggagctgcgccttatccagacgctgatcgcccatcatcgcggttctttagatctct cggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacagcaggttatcaccgggaggcttaaaatga 92 atgacccagttacctaccgcgggcccggttatccggcgctttgatatgtctgcccagtttacggcgctttatcgcatcagcgtggcgctgagtcaggaaagcaataccgcgcgcgcactggcggcgatcctcgaagtgcttcacgatcatgcatttatgcaatacggcatggtgtgtctgttcgataaagaacg caatgcactgtttgtggaatccctgcatggcatcgacggcgaaaggaaaaagagacccgccatgtccgttaccgcatgggggaaggcgtgatcggcgcggtgatgagccagcgtcaggcgctggtgttaccgcgcgcatttcagacgatcagcgttttctcgaccgcctgaatatttacgattacagcctgccgct gattggtgtgccgatccccggtgcggataatcagcctgcgggtgtgctggtggcacagccgatggcgttgcacgaagaccggctggctgccagtacgcggtttttagaaatggtcgccaatctcatcagccagccactgcgttctgccacgcccccggaatcattgcctgctcaaacgccggtccggtgcagtgttccg cgccagtttggttttgagcagatggtcgggaaaagtcaggcgatgcgccagacgatggacattttacggcaggtttccaaatgggataccacggttctggtgcgtggtgaaagcggcaccggcaaggaacttatcgccaatgccattcattacaactcaccccgtgcggccgcgccatttgtgaaattcaactgcgccgcgctg ccggataacctgctggaaagcgaactgttcggtcatgaaaaaggggccttcaccggcgctatacgcacccgaaaaggccgctttgaactggcggacgggggcacgttattcctcgatgaaatcggcgaatcgagcgcgtcgtttcaggccaaattgctgcgcattttgcaggaaggtgaaatggaacgggtcggc ggcgataccacgctgaaagttgatgtgcgcattattgctgccaccaaccgtaatcttgaggaggaagtgcgtgccgggaattttcgcgaagacctgtattatcgcctgaacgtgatgccggtttcgctgcctgcactgcgtgaaaggctggatgatcgccgatctggcgccgtttctggtcaaaaagattg cgctgcgtcaggggcgggaactgcgcatcagtgatggtgcggtgcgtctgctgatgacctacagctggccaggcaacgtgcgtgaactggaaaactgcctcgaacgggcgtcggtaatgaccgatgaagggctgatcgaccgcgacgtgatcctgttcaatcaccatgagtccccggcgctgtccgtcaa acccggtctgccgctggcgacagatgaaagctggctggatcaggaactcgacgaacgccagcgggtgattgctgcactggagaaaaccggctgggtgcaggccaaagcggcccgactgctgggcatgacaccgcgccagattgcctaccgtatccagattatggacatcaacatgcaccgtatctga 93 atgttgccactttcttctgttttgcaaagccacgcgcagagtttgcctgaacgctggcatgaacatcctgaaaacctgcccctccccgatgatgaacagctggctgtgctgagcagcagtgaattcatgacggacagtttgctggcttttccgcagtggtggcatgaaattgtccaaaatccccctcaggcgcaggagtggca actttaccgtcagtggctggatgaatcgctgacgcaggtgactgacgaagccgggttaatgaaagctttgcgtctgttccgccgccgtattctgacccgcattgcgtggtcacagtccgcgcaaaccagcgaagcaaaagatacgcttcaccagctgagtgaactggcggaattattgattgtcagcgcccgtgact ggctgtatgccgcttgctgtcgcgagttgctgtcgcgagttcggtacgccggtcaatgccgcaggggaaccgcagagaatgctgatcctcgggatgggcaaactcggcggtggcgagctgaatttctcatcggacatcgacctgatttttgcttatccggaaaatggccagacacgcggcggtcggcgtgaactggataacgcacaattttt cacccggctcggccagcgtctgatcaaagcgctggatcagcccactatcgacggttttgtctatcgcgtggacatgcgtttgcgtccgttcggcgacagtggcccgctggtgatgagcttcccggcactggaagattattatcaggaacaggggcgcgactgggaacgctacgcaatggtgaaagcgcgtctga tgggcggcgcggaggacatcagcagtcaggaattgcgcgtaaaatgctgatgccttttgtcttccgccgtttatcgatttcagtgtgatccagtccctgcgtaacatgaaaggcatgatcgcccgcgaagtacgccgccgtggtctgaaagaacatcaaactcggcgcaggcggtattcgtgaaattga atttatcgtgcaggtatttcagctgatccgtggcggtcgtgaaccggcattgcagcagcgtgcgttgttgccaacgcttcaggcgctggaaaatctggggctgctgccggtagagcaggtgttgcagttgcgtaacagctatctgttcctgcgacgtctggaaaacctgttgcaggccattgctgacgagca aacgcaaaccttaccgtccgatgagctgaatcaggcgcgtctggcgtgggggatgaattacgctggctggccgcagcttctggatgcagtgaatgctcacatgcaggccgtacgcgcggtatttaacgatctgattggcgatgacacgccagatgccgaagatgacgtgcaactctcccggttcagcagtttatggattgat acgcttgagcctgacgagctggctccgctggtgccgcaacttgacgaaaatgcgcaacggcatgttttacatcagattgctgattttcgccgtgacgtggataaacgcacgatagggccacgtgggcgtgatcagttggatttgctgatgccgcgttactggcccaggtctgcacctataaaaatgcggatgtgac gttacagcgcctgatgcagttgctgctcaatatcgtcacgcgcacgacgtatatcgagctgctggtggaatatcccggtgcgctcaaacagttaatacgtctgtgcgctgcctcgccgatggtggcgacgcaacttgcgcgtcatcctttattgctcgacgaactgctcgacccgcgcacgct ttaccagccgattgagccgggcgcgtaccgtgatgaactgcggcaatatctgatgcgggtgccaaccgaagacgaagaacaacagcttgaagccgtgcgccagttcaaacaggcacagcatttacgtattgcggccggggatatttccggtgcgttgccggtgatgaaagtcagtgaccatttaacctaccttgcggaggccatt ctcgacgttgtggtgcaacaggcgtgggaacaaatggtcgtaaaatacggtcagccaacccatcttcagcaccgtaaagggcgcggttttgccgtggtgggttacggaaaactcggtggctgggagctgggttacagctcggatctggatctggtcttcctgctcgattgcgcgccggaagtcatgaccgacggc gaacgcagcattgacgggcgtcagttttatctgcggctggcgcagcgcatcatgcatttattcagcacccgtacgtcgtcaggcattctttatgaggttgacccgcgtctgcggccttccggtgcttccggcatgctggtcagcaccatcgaagcttttgcggattatcaggccaacgaagcctggacatgggagcatcaggcgct ggttcgcgcgcgtgtggtttatggtgatccgcaactgacgcagcaatttaatgccacgcgtcgcgacattctttgccgccagcgcgatgccgacggcttgcgtaaggaagtccgtgaaatgcgcgagaaaatgtatgcccatctgggcagcaaaagagccgacgagtttgatctgaaagccgatcc gggtggcataacggatattgaattcatcgcacaatatctggttctgcgtttcgcgcatgatgagccgaagctgacccgctggtctgataacgtgcggattttcgaactgatggcgcgacatgacatcatgccggaagaggaagcacgccatctgacgcaggcttacgtgacattgcgcgatgaaattcatcatctggcgttgcagga acacagcgggaaagtggccgcagacagctttgccactgagcgcgcgcaaatccgcgccagctgggcaaactggcttggctga 94 atga aaa tccatgctgactcaggttattgttacctttgctctggtctgcgtcctgtggatcctctacggttacagccttgccttcagtgaaggtaacgcgttcttcggtggtttcagcaacgtaatgatgaaaggcattggcctggattctgtgactggcaccttctcgcagatgatccacgttgcattccagtgttcatttgcctgcat cactgtagcgctgatcgtaggtggtattgctgaacgtgtgcgtttctcagcagttctgattttcactgtgatctggctgactttctcttatattccgatggctcacatggtatgggcaggcggtttcctggctgctgacggtgcgctggactttgccggtggtaccgttgttcatatcaatgccgcaattgctggcctggtaggggct tatctgctgggtaaacgcgccggttttggcaaagaagctttcaaaccacacaacctgccaatggtcttcactggcgcctcaatcctgtatgtgggctggttcggcttcaatgcggttcagcaagtgccgcaagctctgttgccgcgctggctttcctgaacactgtcattgctactgctggcgcaatcctgtcctggacgct ggttgagtggatggtgcgcggtaagccctcactgctgggcgcaagctccggtgctatcgcaggtctggtggctatcacgcctgcatgtggtacggtcggcgtaggtggtgctctgattatcggtctggtaggcggtatcactggtctgtggggggttgttaccctgaaaaaatggctgcgtgttgatgacacctg tgatgtgttcggtgttcatggcgtgtgcggtatcgtaggttgtctgctgacgggtgtattcacgtccagttcacttggcggcgtgggctacgcagaaggcgtgaccatgggccatcaggtttgggtgcagttcttcagcgtgtgcgtaacattggtctggtcaggcgttgttgccttcatcggt tacaaagtggctgacatgatcgtaggtctgcgtgttcctgaagaacaagaacgcgaaggtctggacgttaacagccacggcgaaaacgcttacaaccaataa 95 tgaatatcactgactcacaagctacctatgtcgaagaattaactaaaaaactgcaagatgcaggcattcgcgttaaagccgacttgagaaatgagaagattggctttaaaattcgcgaacacacgctacgccgtgttccttatgttagtttgtggcgataaagaggtcgaagcaggcaaagttgctgttcgtactcgtc gcggcaaagacttaggaagcatggatgttagcgaagtcgttgacaaactgctggcggaaatccgcagcagaagtcatcaactggaggaataaagtattaaaggcggaaaacgagttcaaccggcgcgtcctaatcgcattaacaaagagattcgcgcgcaagaagttcgcctcaccggcgtcgatggcgagcagattggtattg tcagtctgaatgaagctcttgaaaaagctgaggaagcgggcgtcgatttagtagaaatcagtccgaatgccgagccgccagtttgtcgaatc 96 attgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgagcggtagcacagagagcttgctctcgggtgacgagcggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataccgcataacgtcgcaagaccaaagtgggggaccttcgggcct catgccatcagatgtgcccagatgggattagctagtaggtggggtaacggctcacctaggcgacgatccctagctggtctgaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtgtgaagaaggccttcgggttg taaagcactttcagcggggaggaaggcggtgaggttaataacctcaccgattgacgttacccgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggtctgtcaagtcggatgtgaaatccccgggctcaacctgggaactgcatt cgaaactggcaggctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgctgtaaacgatgtcgatttggaggttgtgcccttgagg cgtggcttccggagctaacgcgttaaatcgaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctggtcttgacatccacagaactttccagagatggattggtgccttcgggaactgtgagacaggtgctg catggctgtcgtcagctcgtgttgtgaaatgttggg 97 atgaccatgcgtcaatgcgctatctacggtaaaggcggtatcggtaaatccaccaccacccagaatctcgtcgcggccctcgccgagatgggtaagaaagtgatgatcgtcggctgcgatccgaaagcggattccacccgtctgatcctccacgctaaagcccagaacaccatcatggagatggcggcggaagtgggctc ggtcgaggatctggagctcgaagacgttctgcaaatcggctatggcgatgtccgttgcgccgaatccggcggcccggagccaggcgtcggctgcgccggacgcggggtgatcaccgccatcaacttcctcgaggaagaaggcgcctatgaagaagatttggatttcgtcttctatgacgtcctcggcgacgtggtctg cggcggcttcgctatgccgatccgcgaaaacaaagcccaggagatctacatcgtctgctccggcgagatgatggcgatgtatgccgccaacaatatctccaaagggatcgtgaagtacgccaaatccggcaaggtgcgcctcggcggcctgatctgtaactcgcgcaaaaccgaccgggaagacgaactgatcatcg ccctggcggagaagcttggcacgcagatgatccacttcgttccccgcgacaacattgtgcagcgcgcggagatccgccggatgacggtgatcgagtacgacccgacctgtcagcaggcgaatgaatatcgtcaactggcgcagaagatcgtcaataacaccaaaaaagtggtgccgacgccgtgcaccatggacga gctggaatcgctgctgatggagttcggcatcatggaagaagaagacaccagcatcattggtaaaaccgccgctgaagaaaacgcggcctga 98 atggttaggaaaagtagaagtaaaaatacaaatatagaactaactgaacatgaccatttattaataagtcaaataaaaaagcttaaaacacaaaccacttgcttttttaataataaaggaggggttgggaagactacattagtagcaaatttaggagcagagctatcaataaactttagtgcaaaagttcttattgtggatgccgaccctcaatgtaatctcacg cagtatgtattaagtgatgaagaaactcaggacttatatgggcaagaaaatccagatagtatttatacagtaataagaccactatcctttggtaaaggatatgaaagtgacctccctataaggcatgtagagaatttcggttttgacataattgtcggtgaccctagacttgctttacaggaagacctttagctggagactggcgagatgccaaaaggcggtgggatg cgaggaattaggacaacttttgtatttgcagagttaattaagaaagctcgtgagctaaattatgattttgttttttgacatgggaccatcattaggcgcaatcaacagggcagtattactggcaatggaattctttgtcgtcccaatgtcaatcgatgtattttcactatgggctattaaaaatttggctccacggtttcaatatggaaaaaaga attagacacagggattcggctctcagaggaacctagcgaattatcacaattatcacctcaaggaaaactaaagtttctcggttacgtcacccaacaacataaagaacgctctggatacgatacaattcagcttgagaatactgaggaagaaataaaatcgaaacgtcgggtaaaggcgtatgaagacattggagaggtgtttccttctaaaattactgag catctttctaaactttatgcatcaaaagatatgaacccacaccttggagatatacgtcatttaggtagtttagctccgaaatcacaatcacaacacgttccgatgatatcagtgtctctggtacaggaaattacaccagacttagaaaaagcgcgcgtgaactttatcgagatattgcaagaagatacttagagaacattcagactgctaatggcgagaaaatag 99 atgaagggaaaggaaattctggcgctgctggacgaacccgcctgcgagcacaaccagaagcaaaaatccggctgcagcgcccctaagcccggcgctaccgccggcggttgcgccttcgacggcgcgcagataacgctcctgcccatcgccgacgtcgcgcacctggtgcacggccccatcggctgcgcgg gcagctcgtgggataaccgcggcagcgtcagcgccggcccggccctcaaccggctcggctttaccaccgatcttaacgaacaggatgtgattatgggccgcggcgaacgccgccgcctgttccacgccgtgcgtcacatcgtcgaccgctatcatccggcggcggtctttatctacaacacctgcgtaccggcgatggag ggcgatgacatcgaggcggtgccaggccgcacagaccgccaccggcgtcccggtcatcgctattgacgccgccggtttctacggcagtaaaaatcttggcaaccgaatggcgggcgacgtgatgctcaggcaggtgattggccagcgcgaaccggccccgtggccagacaacacgccctttgccccggcccagcgccacgata tcggcctgattggcgaattcaatatcgccggcgagttctggcaggtccagccgctgctcgacgagctggggatccgcgtcctcggcagcctctccggcgacggccgctttgccgagatccagaccctgcaccgggcgcaggccaatatgctggtgtgctcgcgcgcgctgatcaacgtcgcccgggggctggagct gcgctacggcacgccgtggtttgaaggcagcttcttacgggatccgcgccacctccgacgccttgcgccagctggcgacgctgctgggggatgacgacctgcgccgccgcaccgaggcgctgatcgcccgcgaagagcaggcggcggagcaggctctgcgccgtggcgtgagcagctccgcgggcgca aagtgctgctctctataccggcggcgtgaaatcctggtcggtggtatcggccctgcaggatctcggcatgaccgtggtggccaccggcacgcgcaaatccaccgaggaggacaaacagcggatccgtgagctgatgggcgacgaggcggtgatgcttgaggagggcaatgcccgcaccctgctcgacgtggtgtaccgctat caggccgacctgatgatcgccggcggacgcaatatgtacaccgcctggaaagcccggctgccgtttctcgatatcaatcaggagcgcgagcacgcctacgccggctatcagggcatcaccctcgcccgccagctctgtctgaccctcgccagccccgtctggccgcaaacgcatacccgcgccccgtggcgctag 100 atgaccaacgcaacaggcgaacgtaaccttgcgctcatccaggaagtcctggaggtgtttcccgaaaccgcgcgcaaagagcgcagaaagcacatgatgatcagcgatccgcagatggagagcgtcggcaagtgcattatctcgaaccgtaaatcgcagcccggggtgatgaccgtgcgcggctgcgcc tatgcgggctcgaaaaggggtggtgtttgggccaatcaaagacatggcccatatctcgcacggccccatcggctgcggccagtattcccgcgccggacggcgcaactactataccggcgtcagcggtgtcgacagcttcggcaccctgaacttccctctgattttcaggagcgcgatattgttttcggcggcgataaaaagctga ccaaactgatcgaagagatggagctgctgttcccgctgaccaaagggatcaccatccagtcggagtgcccggtgggcctgatcggcgatgacatcagcgccgtagccaacgccagcagcaaggcgctggataaaccggtgatcccggtgcgctgcgaaggctttcgcggcgtatcgcaatcgctgggccaccatatcgcca . cccattcgttaagcttaacctcgtccactgctaccgttcgatgaactatatcgcccgccatatggaggagaaacatcagatcccatggatggaatataacttcttcggcccgaccaaaatcgccgaatcgctgcgcaagatcgccgatcaatttgatgacaccattcgcgccaatgcggaagcggtgatcgccaaatatgaggggca gatggcggccatcatcgccaaatatcgccgcggctggaggggcgcaaagtgctgctgtacatgggggggctgcggccgcgccacgtcatcggcgcctatgaggatctcgggatggagatcatcgccgccggctacgagtttgcccataacgatgattacgaccgcaccctgccggacctgaaagagggcaccctgctgtttgac gatgccagcagctatgagctggaggccttcgtcaaagcgctgaaacctgacctcatcggctccgggatcaaagagaaattatcttccagaaaatgggggtgccgttccgccagatgcactcctggggactattccggcccctatcacggctatgacggcttcgccatcttgcccgcgatatggatatgaccctgaacaatccggcg tggaacgaactgactgccccgtggctgaagtctgcgtga 101 atggcagatattatccgcagtgaaaaaccgctggcggtgagcccgattaaaaccgggcaaccgctcggggcgatcctcgccagcctcgggctggcccaggccatcccgctggtccacggcgcccagggctgcagcgccttcgccaaagttttctttattcagcatttccatgacccggtgccgctgcagtcgacgg ccatggatccgaccgccacgatcatggggccgacggcaatatcttcaccgcgctcgacaccctgccagcgccacagcccgcaggccatcgtgctgctcagcaccggtctggcggaagcgcagggcagcgatatcgcccgggtggtgcgccagtttcgcgaggcgcatccgcgccataacggcgtggcgatcct caccgtcaataccccggatttttttggctctatggaaaacggctacagcgcggtgatcgagcagtgggtcgcgccgacgccgcgtccggggcagcggccccggcgggtcaacctgctggtcagccacctctgttcgccaggggatatcgaatggctgggccgctgcgtggaggcctttggcct gcagccggtgatcctgccggacctcgcagtcaatggatggccacctcggtgaaggggattttacgcccctgacccagggcggcgcctcgctgcgccagattgcccagatgggccagagtctgggcagcttcgccattggcgtgtcgctccagcgggcggcatcgctcctgacccaacgcagccgcggcgacgt gatcgccctgccgcatctgatgaccctcgaccattgcgatacctttatccatcagctggcgaagatgtccggacgccgcgtaccggcctggattgagcgccagcgtggccagctgcaggatgcgatgatcgactgccatatgtggcttcagggccagcgcatggcgatggcggcggagggcgacctgctggcggcgtgg tgtgatttcgcccgcagccaggggatgcagcccggcccgctggtcgcccccaccagccaccccagcctgcgccagctgccggtcgagcaagtcgtgccgggggatcttgaggatctgcagcagctgctgagccaccaacccgccgatctgctggtggctaactctcacgcccgcgatctggcggagcagtttgccctg ccgctgatccgcgtcggttttcccctcttcgaccggctcggtgagtttcgtcgcgtccgccaggggtacgccggtatgcgagatacgctgtttgaactggccaatctgctgcgcgaccgccatcaccacaccgccctctaccgctcgccgcttcgccagggcgccgacccccagccggcttcaggagacgcttatg ccgcccattaa 102 atgagccaaacgatcgataaaattcacagctgttatccgctgtttgaacaggatgaataccagaccctgttccagaataaaaagacccttgaagaggcgcacgacgcgcagcgtgtgcaggaggttttgcctggaccaccaccgccgagtatgaagcgctgaacttccagcgcgaggcgctgaccgtcgacccggccaaag cctgccagccgctcggcgccgtactctgcgcgctggggttcgccggcaccctgccctacgtgcacggctcccagggctgcgtcgcctattttcgcacctactttaaccgccattttaaagagccggtcgcctgcgtctccgactccatgaccgaggacgcggcggtgttcggcggcaacaacaacatgaatctggg cctgcagaatgccagcgcgctgtataaacccgagattatcgccgtctccaccacctgtatggccgaggtgatcggcgacgatctgcaggcgtttcgccaacgccaaaaaagagggattgttgacgaccgcatcgccattccttacgcccatacccccagctttatcggcagccatgtcaccggctgggacaatatgttcgaaggg ttcgcgaagacctttaccgctgactacgccgggcagccgggcaaacagcaaaagctcaatctggtgaccggatttgagacctatctcggcaacttccgcgtgctgaagcggatgatggcgcagatggatgtcccgtgcagcctgctctccgacccatcagaggtgctcgacaccccgccgacggccattaccggatgtacgccggc ggcaccagccagcaggagatcaaaaccgcgccggacgccattgacaccctgctgctgcagccgtggcagctggtgaaaagcaaaaaggtggttcaggagatgtggaaccagcccgccaccgaggtggccgttccgctgggcctggccgccaccgacgcgctgctgatgaccgtcagtcagctgaccggcaaaccgatcg ccgacgctctgaccctggagcgcggccggctggtcgacatgatgctggattcccacacctggctgcatggcaaaaaattcggcctctacggcgatccggatttcgtgatggggctgacgcgcttcctgctggagctgggctgcgagccgacggtgatcctcagtcataacgccaataaacgctggcaaaaagcgatgaaga aaatgctcgatgcctcgccgtacggtcaggaaagcgaagtgttcatcaactgcgacctgtggcacttccggtcgctgatgttcacccgtcagccggactttatgatcggtaactcctacggcaagtttatccagcgcgataccctggcaaagggcaaagccttcgaagtgccgctgatccgtctgggctttccgctgtt cgaccgccatcatctgcaccgccagaccacctggggctatgaaggcgcaatgaacatcgtcacgacgctggtgaacgccgtgctggaaaaactggaccacgacaccagccagttgggcaaaaccgattacagcttcgacctcgttcgttaa 103 atgaccctgaatatgatgctcgataacgccgcgccggaggccatcgccggcgcgctgactcaacaacatccggggctgttttttaccatggtggaacaggcctcggtggccatctccctcaccgatgccagcgccaggatcatttacgccaacccggcgttttgccgccagaccggctattcgctggcgcaattgttaaaccagaaccc gcgcctgctggccagcagccagacgccgcgcgagatctatcaggagatgtggcataccctgctccagcgtcagccctggcgcggtcagctgattaatcagcgtcgggacggcggcctgtacctggtggagattgacatcaccccggtgcttagcccgcaaggggaactggagcattatctggcgatgcagcgggatatcagcg tcagctacaccctcgaacagcggctgcgcaaccatatgaccctgatggaggcggtgctgaataatatccccgccgccgtggtagtggtggacgagcaggatcgggtggtgatggacaacctcgcctacaaaaccttctgcgctgactgcggcggccgggagctgctcaccgagctgcaggtctcccctggccggatgacgccc ggcgtggaggcgatcctgccggtggcgctgcgcggggccgcgcgctggctgtcggtaacctgctggccgttgcccggcgtcagtgaagaggccagccgctactttatcgacagcgcgctggcgcggaccctggtggtgatcgccgactgtacccagcagcgtcagcagcaggagcaagggcgccttgacc ggctgaagcagcaaatgaccgccggcaagctgctggcggcgatccgcgagtcgctggacgccgcgctgatccagctgaactgcccgattaatatgctggcggcagcccgtcggctgaacggcgagggaagcgggaatgtggcgctggaggccgcctggcgtgaaggggaagaggcgatggcgcggctccagcgctgtcg cccatcgctggaactcgaaaaccccgccgtctggccgctgcagccctttttcgacgatctgtgcgccctctaccgtacacgcttcgatcccgacgggctgcaggtcgacatggcctcaccgcatctgatcggctttggccagcgcaccccactgctggcgtgcttaagcctgtggctcgatcgcaccctggccctcg ccgccgaactcccctccgtgccgctggcgatgcagctctacgccgaggagaacgacggctggctgtcgctgtatctgactgacaacgtaccgctgctgcaggtgcgctacgctcactcccccgacgcgctgaactcgccgggcaaaggcatggagctgcggctgatccagaccctggtggcgcaccatcgcggggccattgagct ggcttcccgaccgcagggcggcacctgcctgaccctgcgtttcccgctgtttaacaccctgaccgggaggtgaagcatga 104 atgatccctgaatccgacccggacaccaccgtcagacgcttcgacctctctcagcagttcaccgccatgcagcggataagcgtggtgctgagccgggccaccgaggccagcaaaacgctgcaggaggtgctcagcgtattacacaacgatgcctttatgcagcacgggatgatctgcctgtacgacagcgagcaggagat cctcagtatcgaagcgctgcagcaaaccggccagcagcccctccccggcagcacgcagatccgctatcgccccggcgagggactggtggggaccgtgctggcccaggggcagtcgctggtgctgccccgggtcgccgacgatcagcgttttctcgaccgcctgagcctctacgattacgatctgccgtttatcg ccgtaccgttgatggggcccaacgcccggccaataggggtgctggcggcccagccgatggcgcgccaggaagagcggctgccggcctgcacccgttttctcgaaaccgtcgccaacctcgtcgcccagaccatccggctgatgatccttccggcctcacccgccctgtcgagccgccagccgccgaaggtggaacggccgcc ggcctgctcgtcgtcgcgcggcgtgggccttgacaatatggtcggcaagagcccggcgatgcgccagatcgtggaggtgatccgtcaggtttcgcgctgggacaccaccgtgctggtacgcggcgaaagcggcaccgggaaagagctgatcgccaacgccatccatcaccattcgccacgggctggcgccg ccttcgtcaaatttaactgcgcggcgctgccggacaccctgctggaaagcgaactgttcggccatgagaaaggcgcctttaccggggcggtgcgtcagcgtaaaggacgttttgagctggcggatggcggcaccctgttcctcgatgagattggtgaaagcagcgcctcgttccaggccaagctgctgcgtatcc tccaggagggggagatggagcgggtcggcggcgatgagaccctgcgggtgaatgtccgcatcatcgccgccaccaaccgtcacctggaggaggaggtccggctgggccatttccgcgaggatctctactatcgtctgaacgtgatgcccatcgccctgcccccgctgcgcgagcgtcaggaggacatcgccgagctggcgc acttcctggtgcgcaaaatcggccagcatcaggggcgcacgctgcggatcagcgagggcgcgatccgcctgctgatggagtacagctggccgggtaacgttcgcgaactggagaactgcctcgaacgatcggcggtgatgtcggagagtggcctgatcgatcgcgacgtgatcctcttcactcaccaggatcg tcccgccaaagccctgcctgccagcggggccagcggaagacagctggctggacaacagcctggacgaacgtcagcgactgatcgccgcgctggaaaaagccggctgggtgcaggccaaggcggcacggctgctggggatgacgccgcgccaggtcgcttatcggatccagatcatggatatcaccctgccgcgtctgtag 105 atgatgccgctttctccgcaattacagcagcactggcagacggtcgctgaccgtctgccagcggattttcccattgccgaactgagcccacaggccaggtcggtcatggcgttcagcgattttgtcgaacagagtgtgatcgcccagccgggctggctgaatgagcttgcggactcctcgccggaggcggaagagtggcggcat tacgaggcctggctgcaggatcgcctgcaggccgtcactgacgaagcggggttgatgcgagagctgcgtctcttccgccgccagatgatggtccgcatcgcctgggcgcaggcgctgtcgctggtgagcgaagaagagagactctgcagcagctgagcgtcctggcggagaccctgattgtcgccgcccgcgact ggctgtacgccgcctgctgtaaggagtggggaacgccatgcaatgccgagggccagccgcagccgctgctgatcctcgggatgggaaagctgggcggcggcgagctgaacttctcttccgatatcgatctgatctttgcctggcctgagcatggcgccacccgcggcggccgccgcgagctggataacgcccagttcttt acccgtctggggcagcggctgatcaaggcccttgaccagccgacgcaggacggctttgtctatcgggttgacatgcgcctgcggccgttggcgacagtgggccgctggtactcagtttgcggcgctggaagattattaccaggagcagggtcgggactgggaacgctatgcgatggtgaaagcgcggatcatgggcgataacg . tatcgttcaggtctttcaactgatccgcggtggtcgcgaacctgcactgcagcagcgcgccctgctgccgacgctggcggcgattgatgagctacatctgctgccggaaggcgacgcggcgctgctgcgcgaggcctatctgttcctgcgccggctggaaaacctgctgcaaagcatcaacgatgagcag acccagaccctgccgcaggatgaacttaaccgcgccaggctggcgtgggggatgcataccgaagactgggagacgctgagcgcgcagctggcgagccagatggccaacgtgcggcgagtgtttaatgaactgatcggcgatgatgaggatcagtccccggatgagcaactggccgagtactggcgcgagctgtggcaggatgcgct ggaagaagatgacgccagcccggcgctggcgcatttaaacgataccgaccgccgtagcgtgctggcgctgattgccgattttcgtaaagagctggatcggcgcaccatcggcccgcgcggccgccaggtgctggatcagctgatgccgcatctgctgagcgaaatctgctcgcgcgccgatgcgccgctgcctct ggcgcggatcacgccgctgttgaccgggatcgtcacccgtaccacctatcttgagctgctgagcgaattccccggcgcgctgaagcacctgatcacgctctgcgcggcgtcgccgatggtcgccagccagctggcgcgccacccgctgctgctggatgagctgctggatcccaacaccctctatcagccgacgg cgaccgatgcctatcgcgacgagctgcgccagtacctgctgcgcgtgccggaagaggatgaagagcagcagctggaggcgttgcgccagtttaagcaggcgcagcagctgcatatcgcggcggcggatatcgctggtaccctgccggtgatgaaggtcagcgatcacttaacctggcttgccgaagcgatcct cgacgcggtggtgcagcaggcatgggggcagatggtcgctcgctacggccagccgacccacctgcacgatcgccagggtcgcggcttcgccgtcgtcggctacggtaagcttggcggctgggagctgggctacagctccgatctcgatctggtgttcctccatgactgcccggcggaggtgatgaccgacggcga gcgggagattgacggccgtcagttctacctgcggctggcccagcggatcatgcacctgttcagcacccgcacctcgtccggtattctctacgaagtggacgcccggctgcgtccttctggcgcggcggggatgctggtcaccaccgccgacgcgtttgctgactatcagcagcagaacgaagcctggacgtgggaacatcaggcg ctggtgcgcgcccgcgtggtctatggcgacccggcgctgcaggcgcgctttgacgccattcgtcgcgatatcctgaccaccccgcgggaggggatgaccctgcagaccgaggttcggagatgcgcgagaagatgcgcgcccaccttggcaacaaacatcccgatcgttttgatatcaaagccgatgccgg cgggatcaccgatattgaatttattactcagtatctggtcctacgctatgccagtgacaagccgaagctgacccgctggtctgacaacgtgcgtattcttgagctgctggcgcagaacgacatcatggacgaggaggaggcgcgcgccttaacgcatgcgtacaccaccttgcgtgatgcgctccatcacctggccctgcaggagcag ccgggacacgtggcgccagaggccttcagccgggagcgtcagcaggtcagcgccagctggcagaagtggctgatggcttaa 106 agcgtcaggtaccggtcatgattcaccgtgcgattctcggttccctggagcgcttcattggcatcctgaccgaagagttcgctggcttcttcccaacctggattgcaccagtgcaggtagtggtcatgaatattaccgattctcaggctgaatacgttaacgaattgacgcgtaaactacaaaatgcgggcattcgtgtaaaagca gacttgagaaatgagaagattggctttaaaatccgcgagcacactttacgtcgtgtcccgtatatgttggtctgtggcgacaaagaagtcgaagccggcaaagtggccgtgcgcacccgtcgcgggaaagacctcggcagcatggacgtaagtgaagtgattgagaagctgcaacaagagattcgcagccgcagtct tcaacaactggaggaataaggtattaaaggcggaaaacgagttcaaacggcacgtccgaatcgtatcaatggcgagattcgcgccctggaagttcgc 107 atgaaaatggcaacaatgaaatcgggtctgggggcattagcccttcttccgggactggcaatggccgcgcccgcagtggcggacaaagccgataacgcgttttgatgatttgcaccgcgctggttctgtttatgaccatcccggggatcgcgctgttttacggcggcctgatccgcggcaaaaacgtccttttccat gctgactcaggtgattgtgacctttggcctggtctgcgtactgtgggtgatttatggctataccctggccttcggcaccggcggcagcttcttcggtagttttgactgggtgatgctgaaaaatattgaactgaaagcgctgatgggcaccttctatcagtacatccacgtggccttccagggctcgttcgcctgtatcaccgtc gggctgatcgtgggggcgctggctgagcgtattcgtttctccgccgtgctgatttttgtggtggtgtggatgacgctctcttatgttccgattgcgcacatggtctggggcggcggtctgctggcgacccacggcgcgctggacttcgcgggcggcaccgttgtacacatcaacgccgcggttgccgggctgg tgggtgcgtacatgatgggcaaacgtgtgggcttcggcaaagaagcgttcaaaccgcacaatctgccgatggtgttcaccggaaccgccatcctctacgtgggctggttcggcttcaacgccggctccgccagcgcagcgaacgaaattgccgcattggctttcgtcaacaccgtcgtcgccacagcggct gccatcctggcgtggacctttggcgaatgggccctgcgcggtaaaccttcactgctgggcgcctgctccggggcgattgccggtctggttggcgtcacaccagcctgtgggtatatcggtgtcggtggggcgttgattgtgggtatcgcatctggtctggcgggcatctggggcgtaacggcgctgaaacgctgg ctgcgggttgatgacccttgcgacgtcttcggcgtccacggcgtctgcggcatcgtcggctgtatcctgaccggtatcttcgcggccacctctctgggcggcgtgggttatgcagaaggcgtcaccatgggccatcagctgctggtgcaactcgagagtatcgcgattaccatcgtctggtcgggcg ttgtcgctttcattggctacaaagtggcggacatgaccgtggggctgcgcgtaccagaagagcaggagcgcgaaggactggacgtcaacagccatggcgaaaacgcctacaacgcctga 108 cgccgtcctcgcagtaccattgcaaccgactttacagcaagaagtgattctggcacgcatggaacaaattcttgccagtcgggctttatccgatgacgaacgcgcacagcttttatgagcgcggagtgttgtatgatagtctcggtctgagggcattagcgcgaaatgatttttcacaagcgctggcaatccgacccga tatgcctgaagtattcaattacttaggcatttacttaacgcaggcaggcaattttgatgctgcctatgaagcgtttgattctgtacttgagcttgatc 109 gctaaagttctcggctaatcgctgataacatttgacgcaatgcgcaataaaagggcatttgatgccctttttgcacgctttcataccagaacctggctcatcagtgatttttttgtcataatcattgctgagacaggctctgaagagggcgtttatacaccaaaccattcgagcggtagcgcgacggcaagtcagcgttctcctttg caatagcagggaagaggcgccagaaccgccagcgttgaagcagtttgaacgcgttcagtgtataatccgaaacttaatttcggttggga 110 gcccgctgaccgaccagaacttccaccttggactcggctatacccttggcgtgacggcgcgcgataactgggactacatccccattccggtgatcttaccattggcgtcaataggttacggtccggcgactttccagatgacctatattcccggcacctacaataacggtaacgttacttcgcctgggctcgtatacagttttaattcgctaagt cttagcaataaatgagataagcggtgtgtcttgtggaaaaacaaggactaaagcgttacccactaaaaaagatagcgacttttttcactttttagcaaagttgcactggacaaaaggtaccacaattggtgtactgatactcgacacagcattagtgtcgatttttcatataaaggtaattttg 111 ttgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgagcggtagcacagagagcttgctctcgggtgacgagcggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataccgcataacgtcgcaagaccaaagtgggggaccttcggggcct catgccatcagatgtgcccagatgggattagctngtaggtggggtaacggctcacctaggcgacgatccctagctggtctgaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtgtgaagaaggccttcgggttg taaagcactttcagcggggaggaaggcgntnaggttaataaccttgtcgattgacgttacccgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggtctgtcaagtcggatgtgaaatccccgggctcaacctgggaactg cattcgaaactggcaggctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgctgtaaacgatgtcgatttggaggttgtgccctt gaggcgtggcttccggagctaacgcgttaaatcgaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctggtcttgacatccacagaactttccagagatggattggtgccttcgggaactgtgagacaggtg ctgcatggctgtcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatcctttgttgccagcggtnnggccgggaactcaaaggagactgccagtgataaactggaggaaggtggggatgacgtcaagtcatcatggcccttacgaccagggctacacacgtgctacaatggcatatacaaagagaagc gacctcgcgagagcaagcggacctcataaagtatgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtagatcagaatgctacggtgaatacgttccgggccttgtacacaccgcccgtcacaccatgggagtgggttgcaaaagaagtaggtagcttaaccttcgggagggcgctta ccactttgtgattcatgactggggtgaagtcgtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 112 atgaccatgcgtcaatgcgctatctacggtaaaggcggtatcggtaaatccaccaccacccagaatctcgtcgcggccctcgccgagatgggtaagaaagtgatgatcgtcggctgcgatccgaaagcggactccacccgtctgatccttcacgctaaagcccagaacaccatcatggagatggcggcggaagtgggctcgg tcgaggatctggagctcgaagacgttctgcaaatcggctatggcgatgtccgttgcgccgaatccggcggcccggagccaggcgtcggctgcgccggacgcggggtgatcaccgccatcaacttcctcgaggaagaaggcgcctatgaggaagatttggatttcgtcttctatgacgtcctcggcgacgtagtctgc ggcggcttcgccatgccgatccgcgaaaacaaagcccaggagatctacatcgtctgctccggcgagatgatggcgatgtatgccgccaacaatatctccaaggggatcgtgaagtacgcgaaatctggcaaggtgcgcctcggcggcctgatctgtaactcgcgcaaaaccgaccgggaagaacgaactgatcatcg ccctggcggagaagcttggcacgcagatgatccacttcgttccccgcgacaacattgtgcagcgcgcggagatccgccggatgacggtgatcgagtacgacccgacctgtcagcaggcgaatgaatatcgtcaactggcgcagaagatcgtcaataacaccaaaaaagtggtgccaacgccgtgcaccatggacgag ctggaatcgctgctgatggagttcggcatcatggaagaagaagacaccagcatcattggtaaaaccgccgctgaagaaaacgcggcctga 113 atgaccaacgcaacaggcgaacgtaaccttgcgctcatccaggaagtcctggaggtgtttcccgaaaccgcgcgcaaagagcgcagaaagcacatgatgatcagcgatccgcagatggagagcgtcggcaagtgcattatctcgaaccgtaaatcgcagcccggggtgatgaccgtgcgtggctgcgcc tatgcgggctcgaaaggggtggtgtttgggccaatcaaagacatggcccatatctcgcacggccccatcggctgcggccagtactcgcgcgccggacggcgcaactactataccggcgtcagcggtgtcgacagcttcggcaccctgaacttcacctctgattttcaggagcgcgatattgttttcggcggcgataaaaagct gaccaaactgatcgaagagatggagctgctgttcccgctgaccaaagggatcaccatccagtcggagtgcccggtgggcctgatcggcgatgacatcagcgccgtggccaacgccagcagcaaggcgctggataaaccggtgatcccggtgcgctgcgaaggctttcgcggcgtatcgcaatcgctgggccaccatatc gccaacgacgtggtgcgcgactgggtgctgaacaatcgcgaagggcagccgttgccagcaccccgtatgatgttgccatcattggcgattacaacatcggcggcgacgcctgggcctcgcgcattctgctggaagagatggggctgcgctagtggcgcagtggtccggcgacggcaccctggtggagatggaga acaccccattcgttaagcttaacctcgtccactgctaccgttcgatgaactatatcgccgccatatggaggagaaacatcagatcccgtggatggaatataacttcttcggcccgaccaaaatcgccgaatcgctgcgcaagatcgccgatcaatttgatgacaccattcgcgccaatgcggaagcggtgatcgccaaatat gaggggcagatggcggccatcatcgccaaatatcgccgcggctggaggggcgcaaagtgctgctgtacatgggggggctgcggccgcgccacgtcatcggcgcctatgaggatctcgggatggagatcatcgccgccggctacgagttgcccataacgatgattacgaccgcaccctgccggacctgaaagagggcaccctgctg tttgacgatgccagcagctatgagctggaggccttcgtcaaagcgctgaaacctgacctcatcggctccgggatcaaagagaaatattcttccagaaaatgggggtgccgttccgccagatgcactcctgggactattccggcccctatcacggctatgacggcttcgccatctttgcccgcgatatggatatgaccctgaacaat ccggcgtggaacgaactgactgccccgtggctgaagtctgcgtga 114 atgaagggaaaggaaattctggcgctgctggacgaacccgcctgcgagcacaaccagaagcaaaaatccggctgcagcgctcctaagcccggcgcaaccgccggcggctgcgccttcgacggcgcgcagataacgctcctgcccatcgccgacgtcgcgcacctggtgcacggccccatcggctgcgcgg gcagctcgtgggataaccgcggcagcgtcagcgccggcccggccctcaaccggctcggctttaccaccgatcttaacgaacaggatgtgattatggggccgcggcgaacgccgcctgttccacgccgtccgtcacatcgtcgaccgctatcatccggcggcggtctttatctacaacacctgcgtaccggcgatggaggg ggatgacctggaggccgtctgccaggccgcacagaccgccaccggcgtcccggtcatcgccattgacgccgccggtttctacggcagtaaaaatcttggcaaccgaatggcgggcgacgtgatgctcaggcaggtgattggccagcgcgaaccggccccgtggccagacaacacgccctttgccccggcccagcgccacgatc ggcctgattggcgaattcaatatcgccggcgagttctggcaggtccagccgctgctcgacgagctggggatccgcgtcctcggcagcctctccggcgacggccgctttgccgagatccagaccctgcaccgggcgcaggccaatatgctggtgtgctcgcgcgcgctgatcaacgtcgcccgggggctggagctgc gctacggcacgccgtggtttgaaggcagcttctacgggatccgcgccacctccgacgccttgcgccagctggcggcgctgctgggggatgacgacctgtgccgccgcaccgaggcgctgatcgcccgcgaagagcaggcggcggagcaggcgctggcgccgtggcgcgagcagctccgtgggcgcaaag tgttgctctctacaccggcggcgtgaaatcctggtcggtggtatcagccctgcaggatctcggcatgaccgtggtggccaccggcacgcggaaatccaccgaggaggacaaacagcggatccgtgagctgatgggcgacgaggcggtgatgcttgaggagggcaatgccgcaccctgctcgacgtggtgtaccgctatca ggccgacctgatgatcgccggcggacgcaatatgtacaccgcctggaaagcccggctgccgtttctcgatatcaatcaggagcgcgagcacgcctacgccggctatcagggcatcatcaccctcgcccgccagctctgtctgaccctcgccagtcccgtctggccgcaaacgcatacccgcgccccgtggcgctag 115 atggcagacattatccgcagtgaaaaaccgctggcggtgagcccgattaaaaccgggcaaccgctcggggcgatcctcgccagcctcgggctggcccaggccatcccgctggtccacggcgcccagggctgcagcgccttcgccaaagttttctttattcagcatttccatgacccggtgccgctgcagtcgacgg ccatggatccgaccgccacgatcatggggccgacggcaatatcttcaccgcgctcgacaccctgccagcgccacagcccgcaggccatcgtgctgctcagcaccggtctggcggaagcgcagggcagcgatatcgcccgggtggtgcgccagtttcgtgaggcgcatccgcgccataacggcgtggcgatcct caccgtcaataccccggatttttttggctcgatggaaaacggctacagcgcggtgatcgagagcgtgatcgagcagtgggtcgcgccgacgccgcgtccggggcagcggccccggcgggtcaacctgctggtcagccacctctgttcgccaggggatatcgaatggctgggccgctgcgtggaggcctttggcc tgcagccggtgatcctgccggacctctcgcagtcaatggatggccacctcggtgaaggggattttacgcccctgacccagggcggcgcctcgctgcgccagattgcccagatgggccagagtctgggcagcttcgccattggcgtgtcgctccagcgggcggcatcgctcctgacccaacgcagccgcggcgacg tgatcgccctgccgcatctgatgaccctcgaccattgcgatacctttatccatcagctggcgaagatgtccggacgccgcgtaccggcctggattgagcgccagcgcggccagctgcaggatgcgatgatcgactgccatatgtggcttcaggggccagcgcatggcgatggcggcggagggcgacctgctggcggcgt ggtgtgatttcgcccgcagccaggggatgcagcccggcccgctggtcgcccccaccagccaccccagcctgcgccagctgccggtcgatcaggtcgtgccgggggatcttgaggatctgcagcagctgctgagccaccaacccgccgatctgctggtggctaactctcacgcccgcgatctggcggagcagtttgccct gccgctgatccgcgtcggttttcccctcttcgaccggctcggtgagtttcgtcgcgtccgccaggggtacgccggtatgcgagatacgctgtttgagctggccaatctgctgcgcgaccgccatcaccacaccgccctctaccgctcgccgcttcgccagggcgccgaccccctgccggcttcaggagacgctta tgccgcccattaa 116 gtgccgctgatccgtctgggctttccgctgttcgaccgccatcatctgcaccgccagaccacctggggctatgaaggcgcaatgaacatcgtcacgacgctggtgaacgccgtgctggaaaaactggaccacgacaccagccagttgggcaaaaccgattacagcttcgacctcgttcgttaa 117 atgaccctgaatatgatgctcgataacgccgcaccgggaggccatcgccggcgcgctgactcaacaacatccggggctgttttttaccatggtggaacaggcctcggtggccatatccctcaccgatgccagcgccaggatcatttacgccaacccagcgttttgccgccagaccggctattcgctggcgcaattgttaaaccagaacccg cgcctgctggccagcagccagacgccgcgcgcgatctatcaggagatgtggcataccctgctccagcgtcagccctggcgcggtcagctgattaatcagcgtcgggacggcggcctgtgcctggtggagattgacatcaccccggtgcttagcccgcaaggggaactggagcattatctggcgatgcagcgggatatcagc gtcagctacaccctcgaacaacggctgcgcaaccatatgaccctgatggaggcggtgctgaataatatccccgccgccgtggtggtggtggacgagcaggatcgggtggtgatggacaacctcgcctacaaaaccttctgcgctgactgcggcggccgggagctgctcaccgagctgcaggtctcccctggccggatgacg cccggcgtggaggcgatcctgccggtagcgctgcgcggggccgcgcctggctgtcggtaacctgctggccgttgcccggcgtcagtgaagaggccagccgctactttatcgacagcgcgctggcgcggaccctggtggtgatcgccgactgtacccagcagcgtcagcagcaggagcaaggacgccttga ccggctgaagcagcaaatgaccgccggcaagctgctggcggcgatccgcgagtcgctggacgccgcgctgatccagctgaactgcccgattaatatgctggcggcagcccgtcggctgaacggcgagggaagcgggaatgtggcgctggaggccgcctggcgtgaaggggaagaggcgatggcgcggctccagcgctgtc gcccatcgctggaactcgaaaaccccgccgtctggccgctgcagccctttttcgacgatctgtgcgccctctaccgtacccgcttcgatcccgacgggctgcaggtcgacatggcctcaccgcatctgatcggctttggccagcgcaccccgctgctggcgtgcttaagcctgtggctcgaccgcaccctggccctc gccgccgaattgccctccgtgccgctggcgatgcagctctatgccgaggagaacgacggctggctgtcgctgtacctgactgataacgtaccgctgttgcaggtgcgctacgcccactcccccgacgcgctgaactcgccgggtaaaggcatggagctgcggctgatccagaccctggtggcgcaccatcgcggggccattga gctggcttcccgaccgcagggcggcacctgcctgaccctgcgtttcccgctgtttaacaccctgaccgggaggtgaagcatga 118 atgatccctgaatccgacccggacaccaccgtcagacgcttcgacctctctcagcagttcaccgccatgcagcggataagcgtggtgctgagccgggccaccgaggccagcaaaacgctgcaggaggtactcactgtattgcacaacgatgcctttatgcagcacgggatgatctgcctgtacgacagcgagcaggagatcc tcagtatcgaagcgctgcagcaaaccggccagcagcccctccccggcagcacgcagatccgctatcgccccggcgagggactggtggggaccgtgctggcccaggggcagtcgctggtgctgccccgggtcgccgacgatcagcgttttctcgaccgcctgagcctctacgattacgatctgccgtttatcgcc gtaccgttgatggggcccaacgcccggccaataggggtgctggcggcccagccgatggcgcgccaggaagagcggctgccggcctgcacccgttttctcgaaaccgtcgccaacctcgtcgcccagaccatccggctgatgatccttccggcctcacccgccctgtcgagccgccagccgccgaaggtggaacggccgccgg cctgctcgtcgtcgcgcggcgtgggccttgacaatatggtcggcaagagcccggcgatgcgccagatcgtggaggtgatccgtcaggtttcgcgctgggacaccaccgtgctggtgcgcggtgaaagcggcaccgggaaagagctgatcgccaacgccatccatcaccattcgccacgggctggcgccg ccttcgtcaaatttaactgcgcggcgctgccggacaccctgctggaaagcgaactgttcggccatgagaaaggcgcctttaccggggcggtgcgtcagcgtaaaggacgttttgagctggcggatggcggcaccctgttcctcgatgagattggtgaaagcagcgcctcgttccaggccaagctgctgcgtatcc tccaggagggggagatggagcgggtcggcggcgatgagaccctgcgggtgaatgtccgcatcatcgccgccaccaaccgtcacctggaggaggaggtccggctgggccatttccgcgaggatctctattatcgtctgaacgtgatgcccatcgccctgcccccgctgcgcgagcgtcaggaggacatcgccgagctggcgc acttcctggtgcgcaaaatcggccagcatcaggggcgcacgctgcggatcagcgagggcgcgatccgcctgctgatggagtacagctggccgggtaacgttcgcgaactggagaactgcctcgaacgatcggcggtgatgtcggagagtggcctgatcgatcgcgacgtgatcctcttcactcaccaggatcg tcccgccaaagccctgcctgccagcggggccagcggaagacagctggctggacaacagcctggacgaacgtcagcgactgatcgccgcgctggaaaaagccggctgggtgcaggccaaggcggcacggctgctggggatgacgccgcgccaggtcgcttaccggatccagatcatggatatcaccctgccgcgtctgtag 119 atgatgccgctttctccgcaattacagcagcactggcagacggtcgctgaccgtctgccagcggattttcccattgcagaactgagcccacaggccaggtcggtcatggcgttcagcgattttgtcgaacagagtgtgatcgcccagccgggctggctgaatgagcttgcggactcctcgccggaggcggaagagtggcggcat tacgaggcctggctgcaggatcgcctgcaggccgtcactgacgaagcggggttgatgcgagagctgcgtctcttccgccgccagatgatggtccgcatcgcctgggcgcaggcgctgtcgctggtgagcgaagaagagaccctgcagcagctgagcgccctggcggagaccctgattgtcgccgcccgcgactgg ctctacgccgcctgctgtaaggagtggggaacgccatgcaatgccgagggccagccgcagccgctgctgctgatcctcgggatgggaaagctggggcggcggcgagctgaacttctcttccgatatcgatctgatctttgcctggcctgagcatggcgccacccgcggcggccgccgcgagctggataacgcccagttctttaccc gtctggggcagcggctgatcaaggcccttgaccagccgacgcaggacggctttgtctatcgggttgacatgcgcctgcggccgtttggcgacagtgggccgctggtactcagctttgcggcactggaagattattaccaggagcagggtcgggactgggaacgctatgcgatggtgaaagcgcggatcatgggcgataacgacggc gtgtacgccagcgagttgcgcgcgatgctccgtcctttcgtcttccgccgttatatcgacttcagcgtgatccagtcgctgcgtaacatgaaaggcatgatcgcccgcgaagtgcggcgtcgcgggctgaaagacaacatcaagctcggcgccggcgggatccgtgaaattgagtttatc gttcaggtctttcagctgatccgcggtggtcgcgaacctgcactgcagcagcgcgccctgctgccgacgctggcggcgattgatgagctacatctgctgccggaaggcgacgcggcgctgctgcgcgaggcctatctgttcctgcgccggctggaaaacctgctgcaaagcatcaacgatgaacagacccaga ccctgccgcaggatgaacttaaccgcgccaggctggcgtgggggatgcataccgaagactgggagacgctgagcgcgcagctggcgagccagatggccaacgtgcggcgagtgtttaatgaactgatcggcgatgatgaggatcagtccccggatgagcaactggccgagtactggcgcgagctgtggcaggatgcgctggaaga agatgacgccagcccggcgctggcgcatttaaacgataccgaccgccgtagcgtgctggcgctgattgccgattttcgtaaagagctggatcggcgcaccatcggcccgcgcggccgccaggtgctggatcagctgatgccgcatctgctgagcgaaatctgctcgcgcgtgccgatgcgccgctgcctctggcg cggatcacgccgctgttgaccgggatcgtcacccgtaccacctatcttgagctgctgagcgaattccccggcgcgctgaagcacctgatcacgctctgcgcggcgtcgccgatggtcgccagccagctggcgcgccacccgctgctgctggatgagctgctggatcccaacaccctctatcagccgacggcgacc gatgcctatcgcgacgagctgcgccagtacctgctgcgcgtgccggaagaggatgaagagcagcagctggaggcgttgcgccagtttaagcaggcgcagcagctgcatatcgcggcggcggatatcgctggtaccctgccggtgatgaaggtcagcgatcacttaacctggcttgccgaagcgatcctcgac gcggtggtgcagcaggcatgggggcagatggtcgctcgctacggtcagccgacccacctgcacgatcgccagggtcgcggcttcgccgttgtcggctacggtaagctcggcggctgggagctgggctacagctccgatctcgatctggtgttcctccatgactgcccggcggaggtgatgaccgacggcgagcg ggagattgacggccgtcagttctacctgcggctggcccagcggatcatgcacctgttcagcacccgcacctcgtccggtattctctacgaagtggacgcccggctgcgtccttctggcgcggcggggatgctggtcaccaccgccgacgcgtttgctgactatcagcagaacgaagcctggacgtgggaacatcaggcgctggt gcgcgcccgcgtggtctatggcgacccggcgctgcaggcgcgctttgacgccattcgtcgcgatatcctgaccaccccgcgggaggggacgaccctgcagaccgaggttcgcgagatgcgcgagaagatgcgcgcccaccttggcaacaaacatcccgatcgtttgatatcaaagccgatgccggcggga tcaccgatattgaatttattactcagtatctggtcctacgctatgccagtgacaagccgaagctgacccgctggtctgacaacgtgcgtattcttgagctgctggcgcagaacgacatcatggacgaggaggaggcgcgcgccttaacgcatgcatacaccaccttgcgtgatgcgctccatcacctggccctgcaggagcagccgggac acgtggcgccagaggccttcagccgggagcgtcagcaggtcagcgccagctggcagaagtggctgatggcttaa 120 atgaaaatggcaacaatgaaatcgggtctgggggcattagcccttcttccgggactggcaatggccgcgcccgcagtggcggacaaagccgataacgcgttttgatgatttgcaccgcgctggttctgtttatgaccatcccggggatcgcgctgttttacggcggcctgatccgcggcaaaaacgtccttttccat gctgactcaggtgattgtgacctttggcctggtctgcgtactgtgggtgatttatggctataccctggccttcggcaccggcggcagcttcttcggtagctttgactgggtgatgctgaaaaatattgaactgaaagcgctgatgggcaccttctatcagtacatccacgtggccttccagggctcgttcgcctgtatcaccgtc gggctgatcgtgggggcgctggctgagcgtattcgtttctccgccgtgctgattttcgtggtggtgtggatgacgctctcttatgttccgattgcgcacatggtctggggcggcggtctgctggcgacccacggcgcgctggacttcgcgggcggcaccgttgtacacatcaacgccgcggttgccgggctgg tgggtgcgtatatgatgggcaaacgtgtgggcttcggcaaagaagcgttcaaaccgcacaatctgccgatggtgttcaccggaaccgccatcctctacgtgggctggttcggcttcaacgccggctccgccagcgcagcgaacgaaattgccgcactggctttcgtcaacaccgtcgtcgccacagcgg cagccatcctggcctggacctttggcgaatgggctctgcgcggcaaaccttcactgctgggcgcctgctccggggcgattgccggtctggttggcgtcacaccagcctgtgggtatatcggtgtcggtggggcgttgattgtgggtatcgcatctggtctggcgggcatctggggcgtaacggcgctgaaacgctgg ctgcgggttgatgacccttgcgacgtcttcggcgtccacggcgtctgcggcatcgtcggctgtatcctgaccggtatcttcgcggccacctctctgggcggcgtgggttatgcagaaggcgtcaccatgggccatcagctgctggtgcaactcgagagtatcgcgattaccatcgtctggtcgggcg ttgtcgctttcattggctacaaagtggcggacatgaccgtggggctgcgcgtaccagaagagcaggagcgcgaaggactggacgtcaacagccatggcgaaaacgcctacaacgcctga 121 ctgaagagtttgatcctggctcagattgaacgctagcgggatgccttacacatgcaagtcgaacggcagcacggacttcggtctggtggcgagtggcgaacgggtgagtaatgtatcggaacgtgcctagtagcgggggataactacgcgaaagcgtagctaataccgcatacgccctacgggggaaagcaggggatcgcaagacctt gcactattagagcggccgatatcggattagctagttggtggggtaanggctcaccaaggcgacgatccgtagctggtttgagaggacgaccagccacactgggactgagacacggcccagactcctacgggaggcagcagtggggaattttggacaatgggggaaaccctgatccagccatcccgcgtgtgcgatgaaggccttcgggttgtaa agcacttttggcaggaaagaaacgtcatgggntaataccccgtgaaactgacggtacctgcagaataagcaccggctaactacgtgccagcagccgcggtaatacgtagggtgcaagcgttaatcggaattactgggcgtaaagcgtgcgcaggcggttcggaaagaaagatgtgtgaaatcccagagcttaactttggaactgcattttta actaccgggctagagtgtgtcagagggaggtggaattccgcgtgtagcagtgaaatgcgtagatatgcggaggaacaccgatggcgaaggcagcctcctgggataacactgacgctcatgcacgaaagcgtggggagcaaacaggattagataccctggtagtccacgccctaaacgatgtcaactagctgttggggccttcgggg ccttagtagcgcagctaacgcgtgaagttgaccgcctggggagtacggtcgcaagattaaaactcaaaggaattgacggggacccgcacaagcggtggatgatgtggattaattcgatgcaacgcgaaaaaccttacctacccttgacatgtctggaattctgaagagattcggaagtgctcgcaagagaaccggaacacaggtg ctgcatggctgtcgtcagctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccttgtcattagttgctacgaaagggcactctaatgagactgccggtgacaaaccggaggaaggtggggatgacgtcaagtcctcatggcccttatgggtagggcttcacacgtcatacaatggtcgggacagagggt cgccaacccgcgagggggagccaatcccagaaacccgatcgtagtccggatcgcagtctgcaactcgactgcgtgaagtcggaatcgctagtaatcgcggatcagcatgtcgcggtgaatacgttcccgggtcttgtacacaccgcccgtcacaccatgggagtgggttttaccagaagtagttagcctaacc 122 ctgaagagtttgatcctggctcagattgaacgctagcgggatgccttacacatgcaagtcgaacggcagcacggacttcggtctggtggcgagtggcgaacgggtgagtaatgtatcggaacgtgcctagtagcgggggataactacgcgaaagcgtagctaataccgcatacgccctacgggggaaagcaggggatcgcaagacctt gcactattagagcggccgatatcggattagctagttggtggggtaanggctcaccaaggcgacgatccgtagctggtttgagaggacgaccagccacactgggactgagacacggcccagactcctacgggaggcagcagtggggaattttggacaatgggggaaaccctgatccagccatcccgcgtgtgcgatgaaggccttcgggttgtaa agcacttttggcaggaaagaaacgtcatgggttaataccccgtgaaactgacggtacctgcagaataagcaccggctaactacgtgccagcagccgcggtaatacgtagggtgcaagcgttaatcggaattactgggcgtaaagcgtgcgcaggcggttcggaaagaaagatgtgtgaaatcccagagcttaactttggaactgcatttt taactaccgggctagagtgtgtcagagggaggtggaattccgcgtgtagcagtgaaatgcgtagatgcggaggaacaccgatggcgaaggcagcctcctgggataacactgacgctcatgcacgaaagcgtggggagcaaacaggattagataccctggtagtccacgccctaaacgatgtcaactagctgttggggccttcg ggccttagtagcgcagctaacgcgtgaagttgaccgcctggggagtacggtcgcaagattaaaactcaaaggaattgacggggacccgcacaagcggtggatgatgtggattaattcgatgcaacgcgaaaaaccttacctacccttgacatgtctggaattcngaagagattnggaagtgctcgcaagagaaccggaacacaggt gctgcatggctgtcgtcagctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccttgtcattagttgctacgaaagggcactctaatgagactgccggtgacaaaccggaggaaggtggggatgacgtcaagtcctcatggcccttatgggtagggcttcacacgtcatacaatggtcgggacagagggg tcgccaacccgcgagggggagccaatcccagaaacccgatcgtagtccggatcgcagtctgcaactcgactgcgtgaagtcggaatcgctagtaatcgcggatcagcatgtcgcggtgaatacgttcccgggtcttgtacacaccgcccgtcacaccatgggagtgggttttaccagaagtagttagcctaaccgnaagg ggggcgattaccacggtaggattcatgactggggtgaagtcgtaacaaggtagccgtatcggaaggtgcggctggatcacctccttt 123 tacggagagtttgatcctggctcaggatgaacgctggcggcgtgcttaacacatgcaagtcgaacggtgaacacggagcttgctctgtgggatcagtggcgaacgggtgagtaacacgtgagcaacctgcccctgactctgggataagcgctggaaacggcgtctaatactggatatgtgacgtggccgcatggtctg cgtctggaaagaatttcggttggggatgggctcgcggcctatcagcttgttggtgaggtaatggctcaccaaggcgtcgacgggtagccggcctgaggggtgaccggccacactgggactgagacacggcccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagcaacgccgcgtgagg gatgacggccttcgggttgtaaaccttttagcagggaagaagcgaaagtgacggtacctgcagaaaaagcgccggctaactacgtgccagcagccgcggtaatacgtagggcgcaagcgttatccggaattattgggcgtaaagagctcgtaggcggtttgtcgcgtctgctgtgaaatccggaggctcaacctccggcc tgcagtgggtacgggcagactagagtgcggtaggggagattggaattcctggtgtagcggtggaatgcgcagatatcaggaggaacaccgatggcgaaggcagatctctgggccgtaactgacgctgaggagcgaaagggtggggagcaaaacggcttagataccctggtagtccaccccgtaaacgttgggaactagttgtggggtccatt ccacggattccgtgacgcagctaacgcattaagttccccgcctggggagtacggccgcaaggctaaaactcaaaggaattgacggggacccgcacaagcggcggagcatgcggattaattcgatgcaacgcgaagaaccttaccaaggcttgacatatacgagaacgggccagaaatggtcaactctttggacactcgtaaaacaggtggtgcatgg ttgtcgtcagctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaaccctcgttctatgttgccagcacgtaatggtgggaactcatgggatactgccggggtcaactcggaggaaggtggggatgacgtcaaatcatcatgccccttatgtcttgggcttcacgcatgctacaatggccggtacaaagggct gcaataccgcgaggtggagcgaatcccaaaaagccggtcccagttcggattgaggtgcaactcgacctcatgaagtcggagtcgctagtaatcgcagatcagcaacgctgcggtgaatacgttcccgggtcttgtacacaccgcccgtcaagtcatgaaagtcggtaacacctgaagccggtggcctaacccttgtggaggg agccgtcgaaggtgggatcggtaattaggactaagtcgtaacaaggtagccgtaccggaaggtgcggctggatcacctccttt 124 attgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgaacggtagcacagagagcttgctctcgggtgacgagtggcggacgggtgagtaatgtctgggaaactgcccgatggagggggataactactggaaacggtagctaataccgcataatgtcgcaagaccaaagagggggaccttcgggcctctt gccatcggatgtgcccagatgggattagcttgttggtgaggtaatggctcaccaaggcgacgatccctagctggtctgaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtgtgaagaaggccttcgggttg taaagcactttcagcggggaggaaggcgatncggttaataaccgtgttgattgacgttacccgcagaagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggtctgtcaagtcggatgtgaaatccccgggctcaacctgggaact gcattcgaaactggcaggcttgagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgacttggaggttgtgcc cttgaggcgtggcttccggagctaacgcgttaagtcgaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctggtcttgacatccacggaattnggcagagatgccttagtgccttcgggaaccgt gagacaggtgctgcatggctgtcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatcctttgttgccagcggtccggccgggaactcaaaggagactgccagtgataaactggaggaaggtggggatgacgtcaagtcatcatggcccttacgaccagggctacacacgtgctacaatggcatata caaagagaagcgacctcgcgagagcaagcggacctcataaagtatgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtggatcagaatgccacggtgaatacgttcccggccttgtacacaccgcccgtcacaccatgggagtgggttgcaaaagaagtaggtagcttaaccttcgg gagggcgcttaccactttgtgattcatgactggggtgaagtcgtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 125 atgaccatgcgtcaatgcgccatttatggcaaaggtgggatcggcaaatccaccaccacgcaaaacctcgtcgccgctctcgcggaaatgggtaaaaaagtgatgatcgtcggctgcgacccgaaagcggactccacccgtctgatcctgcatgcgaaagcacagaacaccattatggagatggccgccgaagt gggttcagtggaagaccttgaactggaagatgtgctgcaaatcggttacggcggcgtgcgttgtgcagaatccggcggcccggagccaggcgtgggttgtgcaggccgcggcgttattaccgccattaacttccttgaagaagaaggcgcctatgtcagcgacctcgactttgtcttctatgacgtcctcggtgacgt ggtctgcggcgggttcgccatgccgattcgtgaaaacaaagcgcaagagatctatatcgtctgctccggggaaatgatggcgatgtatgccgctaacaacatctccaaaggcatcgtgaaatacgctaaatccggcaaggtgcgcctgggcgggctgatttgtaactcccgtcagaccgaccgcgaagatga actgatcatcgcgctggcagaaaaactgggcacccagatgattcactttgtgccacgcgacaacatcgtccagcgcgcggaaattcgccgtatgacggttatcgaatatgacccgaaatgcaaccaggccgacgaataccgcgcgctggcgaacaagatcgtcaacaacaccctgatggtcgtcccgaccccttg caccatggatgaactggaagagctgctgatggaattcggcattatggatgtggaagacgccagcatcatcggtaaaaccgccgccgaagaaaacgcggcctga 126 atgaacgataacgatgtccttttctggcgcatgctggcgctatttcagtgtctgccggaactgcaacccgcgcagatcctggcctggctgacaggagaacgcgacgacgccttaacccggcgtacctcgataagcttaacgtccgcgaactggaagcgaccttcccgtctgaaacggcgatgatgtcgcccgcac gctggagccgcgttaacgcgtgccttcacggtacgctgcccgcacacctgcaggtaaaaagcaccactcgtcaggggcaattacgggtagccttttgttcacaggatggattgctgatcaatggtcattttggtcaggggcggctgttttttatctacgcctttgatgaacagggcggatggctacacgcgttacgccgtcttcc ctcggccccgcaaacccaggagccgaatgaagttcgcgcgcagctcctgagtgattgccacctgctgttttgtgaagccattggcggccctgcggcggcccggctgattcgtcacaatatccacccgatgaaagtgtcgccagggatgtccattgccgcccagtgtgtgccattaccgcactgctgagcggacgtctgcca ccgtggctggcaaaacgtcttgagaaagccaacccgctggaagagcgggtgttttaa 127 atgaagggaaatgacattctcgcgctgctggatgaacccgcctgcgaacacaatcacaaacagaaatccggctgtagcgcccctaaacccggtgccacggcgggcggttgcgcgttcgacggcgcgcaaatcaccctgttgccgctgtcggatgtggcgcacctggtccacggaccgattggctgcacgggaagct cctgggataaccggggcagtatgagctccggccccagtctcaaccggctcggctttaccaccgacctgaacgagcaggatgtcattatggggcgcggcgaacggcggcttttccacgcggtgcgtcatatcgtcaaccgttatcaccctgccgccgtgtttatctataacacctgcgttccggcgatggagggtgatgatattg acgccgtctgtcaggcggcggaaaccgccaccggcgtgccagtgattgccgttgatgccgccgggttctatggcagcaaaaaccttggcaaccgtcgcgggtgaagtgatggttaacaaggtcattggacggcgcccgcccgccccctggccggacgatacccccttcgcgccggaacaccgccacgata tcggcctgattggcgaatttaatatcgccggggagttctggcacgttcagccgctgctcgatgagctgggtattcgcgtgctgggcagcctttccggggatggccgttttagtgaaatccagaccctgcaccacgcgcaggtcaatatgctggtctgctcaagagcgctgatcaatgttgcccgcaccctggaacagcgct atggcaccccctggtttgagggcagtttttacggcgtgcgcgctacctccgatgccctgcgtcaactggcatccctgcttggcgacagcgatctgattgcccgcaccgaagccgttattgccccgaagaagccacggcaaatcaggcgctcgccccgtggcgcgaacggctacagggtcgcaaagtgctgctctatacc ggtggggtgaaatcctggtcggtggtctccgcattgcaggatttagggatgaccgtcgtggcgactggcacccgcaaatctaccgaagaagataagcagcgtattcgcgaattaatgggcgatgacgcgctaatgctggaagaaggcaacgcccgcaccctgctggatgtggtgtaccgctatcaggcggatttgatgatc gctggggggcgtaacatgtataccgcgtacaaagcgcggctgccgtttctggatatcaaccaggagcgtgaacacgcctttgcgggttatcgcggcatcgtcaccctcgcccaacagctttgccagactattgaaagccccgtctggccgcaaacacacgcccgcgcgccgtggcaataa 128 atgagcaatgcaacaggcgaacgtaatctggaaattatccaggaagtgctggagatctttcccgaaaaaacgcgcaaagaacgcagaaagcacatgatggtgaccgacccggagatggaaagcgtcgggaaatgcatcatctctaaccgcaaatcgcagccgggtgtgatgactgtccgcggctgctcctacgccgggg tcgaaaggcgtggtttttgggccgattaaagatatggcccacatctcccacggcccgatcggctgtgggcagtactcccgtgccgggcggcgcaactactaccggggtcagcggcgttgattccttcgggacgctgaactttacctgattttcaggagcgcgatatcgtcttcggcggcgataaaaagctcaccaaact gattgaggagatggaggaactgttcccgctgaccaaaggcatctccattcagtcggagtgcccggtaggtttaatcggtgacgatatcgaagcggtggcgaatgccagtaaaaaagcgctcaacaagccggtgatcccggtgcgttgcgaaggctttcgcggcgtgtcgcagtcgctcggtcaccatatcgccaac gacgttatccgcgactgggtgctggataaccgcgaagggaagcccttcgaatctaccccctatgacgtggccatcatcggcgattacaacatcgggggggatgcctgggcgtcgcgcattctgcttgaagagatggggttacgcgtggtggcgcagtggtccggtgacggcacgctggtagagatggaaaacacccg ttcgtcaagctgaacctggtgcactgctaccgctctatgaactaccatctctcgccatatggaagagaaacacggtatcccgtggatggagtacaacttcttcggcccgaccaaaatcgccgaatcgctgcgtaagatcgccgatcaatttgacgacaccatccgcgccaatgcggaagcggtgatcgccaaatatcaggcgcaa aacgatgcgattatcgccaaataccgcccgcgtctcgaaggccgcaaggtgctgctatatgggtggcctgcgtcctcgccacgtgattggcgcgtatgaggatttgggcatggagattgtcgccgccgggtatgaatttgcccataacgacgattacgaccgcaccctgccggacctcaaagagggcacgctgtt gttcgacgatgccagcagttatgaactggaagccttcgtgaaggcgattaagccggacctcattggctcaggcatcaaggaaaaatacattttccagaaaatggggggtaccgttcgccagatgcactcctgggattactccggcccgtatcacggctatgacggctttgccatcttgcccgcgatatggacatgacgctcaacaatcccg cctggggcgagttgaccgcaccctggctgaaatcagcctga 129 atggcagatatcatccgtaatcagaaaccgctggcggtaagcccggtaaaaagcggccagccgttaggcgccattctggcgagcctcggctttgagcacagtattccactggtgcacggtgcgcagggatgcagcgcgttcgccaaagtgttttttatccaacattttcatgaccctattccgctgcaatccacggcgatggac cccacctcaacggtcatgggggcggacggcaatatccttgccgcgctcaatacgctgtgccagcgcaacaccccgaaagctatcgtcctgttgagtaccggcctgtctgaggcgcagggcagcgatatcagccgcgtggtacgtcagtttcgtgaggattttccccgccacaaaaatatcgccctcctgacggt caacacccccggatttttacggcacgctggagaacggctttagtgcggtggttagtgcggtggtggaaagcgtcatcgaacagtgggtgccggaaaagcctcagcatggcctgcgtaaccggcgggtcaacttgttgttaagtcacctgctgacgcccggtgatgttgagttgctgcgcagctacgtggaggcttttggcctgcaac cggtgatcgtgccggatctttcacagtcgctggatggtcacctggcaagcggtgatttttcgccggtcactcaggggggaacgcccctgcgcattatcgaacagatgggacagagcctgtgcacgtttgctattggcgtgtcgctgtcccgtgcggcatcgctgctggcacagcgtagccgtggcgagg tgatcgtgcttccccatctgatgaccatggaacattgcgaccgttttattcatcaactgaagatcatttccgggcgcgaggttcccgcctggattgagcgccagcgcggacaattgcaggatgcgatgatcgattgtcatatgtggttgcaggatacccggctcgcgctggccgccgagggcgatctgctggcgggct ggtgtgatttcgcccgtagccagggcatgctccccggccccgttgtggcgccggtcagccagccgggcctgcaacagcttcccgtggagaaagtggtcattggcgatctggaagatatgcaggatttactctgcgctatgcctgctgacctgctggtcgccaactcccatgccgcagacctggccgaacaattctccatccgct gatccgcgccgggttccctatcttcgacaggcttggcgaatttcgtcgcgtgcgtcagggataccccggcattcgcgacacgctgtttgagctggcgaacctgatgcgcgaacgtcatcaccacctgcccgtctaccgctcccccctgcgccagcaatttgcccaggacgctgacggaggccgctatgcaacat gttaa 130 atgagccaaactgctgagaaaattgtcacctgtcatccgctgtttgaacaggacgaataccagacgctgtttcgcaataagcgcggtctggaagaggcgcacgacccgcagcgcgtgcaagaggttttgaatggaccaccacggcggagtatgaagcgctgaactttaagcgtgaagcgttaaccgtcgatccggca aaggcctgccagcctttaggatcggtactctgctcgctgggtttgccaatacgctgccttatgtgcacggttcccagggctgtgtggcctatttccgcacctattttaaccgtcatttcaaagagccgatcgcttgcgtttccgactctatgacagaggatgcggcggtcttcggcggcaacaacaaccttaacaccgggg ttgcaaaatgccagcgccctgtacaaaccggaaattgtcgctgtctccactacctgtatggcggaggtcatcggcgatgacctgcaggcctttcgccaacgccaaaaaaggacggggtttattgatgccgccattccggtgccctacgcccatacgccaagttttatcggtagccacatcaccggctgggacaacatgtttgaaggt ttcgcccgggcatttaccgccgatcacgtggcgccgatcacgtggcgcaaccgggcaaactggcgaagctaaacctggtgaccggttttgaaacctatcttggcaattaccgcgtgctcaaacgcatgatggcccagatggaggtgccctgtagcctgctgtctgacccgtctgaggtgttagatacgccagccgacggccactatcgcatgtatgc gggcggcacaacgcaacaagagatgcgcgacgcccccgatgctatcgacaccctgctgctgcaaccctggcatctggtgaagagtaaaaaagtggtgcaggagtcctggggccagcccgccacagaagtgtccatcccaatgggactgaccgggaccgacgaactgctgatggcagtcagtcagttaaccggcaaaccggtgg ccgatgaactgacgctggagcgtgggcgcctggtggatatgattctcgattcacacacctggctgcacggtaagaaattcggtctctacggcgatccggattttgtgatggggctgacgcgtttcctgctggaactgggctgcgagccgacggttatcctctgtcataacggtagcaagcgctggcagaaagcgatgaagaaa atgcttgaggcatcgccctacggtcaggagagcgaagtgttcatcaactgcgatctgtggcatttccgctcgctgatgtttacccgcaaaccggactttatgatcggcaactcgtacgccaaattcatccagcgtgacacgctggcgaaaggcgaacagtttgaagttccgctgatccgtcttggcttccgttgttcgaccg ccaccacctgcatcgccagaccacatggggttatgaaggggcgatgaatatcgtcaccaccctggtcaacgccgtgctggaaaaagtcgaccgcgataccatcaaactgggcaaaacggactacagcttcgaccttgtccgctaa 131 atgacctttaatatgatgctggagaccagcgcaccgcagcacattgcgggcaacctctcacttcaacatcccggactgttttccacgatggttgaacaggctccgatcgcgatttcgctgaccgacccggacgcgaggattctgtacgctaatccggccttttgtcgccagaccggttatagcctggaagagctgctcaaccagaaccatc gcatactggcaagccaacagacgccgcgcagcatttatcaggaactgtggcaaacgctgctgcaacagatgccctggcgcggtcagctcatcaatcgccgtcgggatggcagcctttatctggctgaggtcgatatcaccccggtcgtcaacaaacagggcgaactggaacactacctcgccatgcaacgtgatatcagcgccagct atgcgctcgaacagcgattgcgcaatcacaccaccatgagcgaggcggtgctgaacaacattcctgccgccgtggtggtggtcaacgagcaggaccaggtagtcatggacaacctcgcctacaaaaccttctgtgccgactgcggtggcaaggagctgctcaccgaactggatttctcccggcgcaaaagcgatctctatgcc gggcaaatactgcctgtggtgctgcgcggcgccgtgcgctggctctctgtcacctgctggaccttgccgggggtgagcgaagaagccagccgctactttattgataccgcgctgccccgcaccctggtggtgatcaccgactgcacccagcaacaacaacaggccgaacagggccgtctcgatcgtctcaaacagg agatgaccaccgggaagctgctggccgcgatccgtgaatcgttggatgccgcgctggttcagctaaactgccccatcaatatgctggcggcggcgcgacgtctcaacggtgaagataaccataacgtggcgctggatgccgcgcgtggcgcgagggggaagaggcgctggcccgcctgcaacgctgccgcccttctctcgat ctggaagagagcgcgctgtggcctctgcaaccgctgtttgacgacctgcgcgccctttaccatacccgctataacaatggcgaaaatctgcacgttgaaatggcctctccgcatctggcggggtttggtcagcgcacgcagatccttgcctgtctcagtttgtggctcgaccgtacgctggccctcgccgccg cgctaccggacagaacgctgcatacccagctttacgcccgtgaagaagatggctggctgtccatttggctgacagataatgtgccgctcatcatccatgtgcgatacgcccactccccgatgccctgaacgccccggcaaagggatggagctgcgattgattcaaaccctggttgcccatcatcgcggcgcaatagaactaactacccgccctgaa ggcggtacctgcctgaccctgcgattcccgttatttcattcactgaccgggaggcccacgatga 132 atgacccagcgacccgagtcgggcaccaccgtctggcgttttgatctctcacagcaatttaccgccatgcagcgcatcagcgtggtgttgagtcgcgcaaccgagataagccagacgctgcaggaggtgctgtgtgttctgcataatgacgcatttatgcaacacggcatgctgtgtctgtatgacaaccagca ggaaattctgagtattgaagccttgcaggaggcagaccaacatctgatccccggcagctcgcaaattcgctatcgccctggcgaagggctggtaggagccgtactgtcccagggacaatctcttgtgctgccgcgtgtcgccgacgatcaacgctttctcgacaggcttggcatctatgattacaacctgccgtttatcgccgt ccccttaatggggccaggcgcgcagacgattggcgtgctcgccgcgcagccgatggcgcgtctggagaggagcggcttccttcctgtacgcgctttctggaaaccgtcgccaatctggtcgcacagacagtccggctgatgaccccgcctgccgccgccacaccgcgcgccgcgattgcccagaccgaacgcca gcgcaactgtggcactcctcgccccttcggctttgagaatatggtgggcaaaagcccggccatgcagcagacaatggacattatccgccaggtttcgcgctgggataccacggtactggtgcgcggcgaaagcggcaccggtaaagaacttatcgccaatgctattcatcacaactcccctcgcgccgccgcgccctttgtgaaat ttaactgcgcggcgctaccggatacgctactggagagcgaattgttcggccatgaaaaaggggcgttcaccggcgcggttcgccagcgtaaaggacgttttgaactggccgatggcggcacactgtttcttgatgaaattggcgaaagcagcgcctcgttccaggccaaactgctgcgtattttgcaggagggtgaaat ggagcgcgttggcggcgacgaaaccctgcgcgtcaatgtgcgtatcatcgccgccaccaaccggaatctggaagaagaagaggtgcggatgggcaatttccgcgaggatctctattatcgcctcaacgtaatgcccatctccctgccccgctgcgtgaacgtcaggaggacattgccgagctggcgcactttctggtgcg caaaatcgcccataaccaggggcgtacgctgcgcgcatcagtgatggcgccatccgtctgctgatgggttacaactggcccggtaacgtgcgtgagctggaaaattgcctggaacgttcggcagtgatgtcagaaaacggcctgatcgaccgcgatgtggtgctctttaaccaccgtgagaacacgccaa aactcgctatcgccgccgcgccaaaagaggatagctggcttgatcaaacgctggatgaacgtcaacggctgattgccgcgctggaaaaagccgggtgggtgcaggccaaagcggcgcgtctgctgggtatgacgccccgtcaggtcgcctatcggatacaaattatggatatcagcatgcccaggatgtga 133 atgatgccgcactctccacagctacagcagcactggcaaactgtactggcccgcttgcctgagtcattcagtgaaacaccgcttagtgaacaagcgcagttagtgcttactttcagtgattttgtgcaggatagccttgccgcgcatcctgactggctggctgagctggaaagcgcaccgccacaggcggacgagtggaagca gtatgcgcaaacccttcgcgaatcgctggaaggtgtgggagatgaggcatcattaatgcgtgcgctgcgcctgttccgtcgccatatgatggtgcgcattgcctgggcgcagtcgctggcgctggtggcagaagaatgagacgttgcagcagttgagcgtactggcggagaccctgatcgtcgct gcacgcgactggctttacgatgcctgctgtcgcgagtggggaacgccgtgcaatcagcagggggaaccgcagccgttgctgatcctgggcatgggcaagctgggtggcggggagcttaacttttcgtccgatatcgatctgatttttgcctggccggaaaacggttcaacgcgcggtgggcgacgcgaactt gataacgcccagttttttactcgcttgggacagcgcctgatcaaagtgctcgaccagccgacgcaggatggctttgtctatcgcgtggatatgcggctgcgcccgtttggcgacagcggtccgctggtgctgagttttgccgcgctggaagattattatcaggagcaggggcgcgactgggaacgttatgcgatggt gaaagcccgcattatgggcgataaggacgatgtttacgctggcgaattacgggccatgctgcggccgttcgtcttccgtcgctatatcgatttcagcgttattcagtctctgcgtaacatgaaagggatgattgcccgcgaagtgcgccgccgtggtgaaagataacattaagctgggcgcgggcggcatccg tgagattgagtttatcgttcaggtgttccagttgatacgcggtgggcgcgagccgtcgttgcagtcccgttcactgttaccgacgctggacgctatcgataagctgggtttgctgccgcctggcgatgcaccggcgttacgccaggcctattgtatctgcgccgtctggaaaacctgctgcaaagcatta acgacgaacaaacgcagacgctgccgacagatgaactcaatcgcgcgcgtctggcctgggggatgcgggtcgcagactgggaaaccctgaccgctgagcttgaaaagcagatgtctgccgtacgagggatattcaacaccctgattggcgatgacgaagccgaagagcagggggatgcgctctgcgggcaatggagt gagttgtggcaggatgcgtttcaggaagatgacagcacgcctgtgctggcgcacctttctgacgatgatcgccgccgcgtggtcgcgatgattgctgattttcgcaaagagctggataaacgcaccattggcccacgcggccgccaggtgctcgaccatctgatgccgcatctgttgagtgatgtctgctccc gtgaggatgcccctgtaccgttgtctcgcgtgacgccgctgttaacgggaattgtcacgcgtacgacgtatcttgagctgctcagcgagtttcctggtgcgcgtaagcatctgatttcactctgtgccgcctcgccgatggtggccagtaagctggcgcgctatccgttattgctggatgagttg ctcgatccgaatacccttttcagccccggcgatgaatgcctaccgggatgagctacgtcagtatctgctgcgtgtgccggatgacgatgaagagcagcaactggaggcgttacgccagtttaaacaggctcaattgttgcgtgtggcggcagcagatctggcaggcacactccccgtgatgaaagtgagcgatcact taacatggcttgccgaagccatcattgaagccgtggtacaacaggcgtggagcctgatggtatcgcgttatgggcagccgaaacacttacgcgaccgtgaaggccgtgggtttgcagtggtcggttacggcaaactgggcggttgggagctgggctatagttccgatctggatttgattttccttcatgactgtccggtggacgt gatgactgacggcgagcgggaaatcgatggccgccaattttatctgcgccttgcccagcgcgtgatgcacctgttcagtacgcgcacctcatccgggatcctgtatgaggtagacgcgcgcttgcgcccgtccggtgcggcgggaatgctggtgacctcaaccgaatcctttgccgactaccagcgcacc gaagcctggacctgggaacatcaggcgctggttcgcgcccgcgttgtctatggcgatccacaattaaacgcgcaatttgatgccatccgccgcgatatcaccatgaccgtgcgtaatggtgcaacgttacaaaccgaggtgcgcgagatgcgcgaaaaaatgcgcccacttgagacaataagcacaaggat cgctttgatattaaagccgatgagggtggaattaccgatatcgaatttatcacccagtatctgggctgcgttatgcccatgccaaaccgaaactgacgcgctggtcggacaatgtccgcattctggaagggctggcgcaaaacggcattatggaagagcaggaagcgcaggcacttaccaccgcctatacaacgttgcgtgatga gctgcatcacctggcgctacaggagctgccaggacatgttccgggaggcatgttttgtcgctgaacgcgcgatggtgcgagcctgctggaacaagtggttggtggagccgtgcgaggacgcgtaa 134 atgaagaaagcactattaaaagcgggtctggcctcgctggcattactgccgtgtctggctatggcagccgatccggttgtcgtcgataaagccgacaatgccttttgatgatttgcaccgcgctggtgctgtttatgtcaattccgggcatcgccctgttctatggtggtttaatccgcggtaaaaacgtcctttct atgctgacacaggttgcggttacgttcgcactggtgtgcgtgctgtgggtggtttacggctactctctggcctttggcactggcggcagcttcttcggtagcttcgactgggtgatgctgaaaaatattgagctgaaagcgctgatgggcaccatctatcagtacattcacgttgcgttccagggctcgtttgcctg tattaccgtcggcctgattgtcggtgcgctggcagaacgtatccgtttctccgcagtactgattttcgtcgtggtatggctgacgctgtcctacgtgccgatcgcacacatggtctggggcggcggtctgctggcaacccatggcgccatggattttgcgggcggtacagtcgttcacatcaacgcagccgttg caggcctggtgggtgcttacctgattggcaaacgtgtcggtttcggtaaagaagcgtttaaaccgcacaacctgccgatggtgtttaccggtacggcaatcctctactttggctggttcggattcaacgcggttctgcaagcgcggcgaacgaaattgcgggtctggctttgttaacaccgtcgtggcaacagc gggtgcaatcctctcctgggtcttcggtgagtgggcgctgcgcggcaaaccgtctctgttgggtgcctgttctggtgcgattgctggcctcgtgggtatcaccccggcgtgtggttacgttggtgtgggtggcgcgctgatcgtgggcatcgttgcaggcctggcgggtctgtggggc gttaccgcgctgaaacgctggctgcgtgttgacgacccgtgtgatgtcttcggtgttcacggcgtgtgcggtatcgtaggttgtatcatgacaggtatcttcgcagccacttcactgggcggcgtgggttatgccgaaggcgtgaccatgggccatcaggttctggtacaactggaaagtatcgccatt actatcgtatggtctggtatcgtcgcctttatcggttacaaactggctgatatgacagtgggtctgcgtgttccggaagatcaggaacgcgaagggctggacgtcaacagccacggcgagaacgcctacaacgcctga 135 ctggggtcactggagcgctttatcggcatcctgaccgaagaatttgccggtttcttcccgacctggctggcccctgttcaggttgtggtgatgaatatcactgattctcaagctgaatatgtcaacgaattgacccgtaaattgcaaaatgcgggcattcgtgtaaaagcggacttgagaaacgagaagattggctttaaaatcc gcgagcacactttacgtcgtgtcccttatatgttggtctgtggtgataaagaggtggaagcaggcaaagtggccgttcgcacccgccgcggtaaagacctgggcagcctggacgtaagtgaagtgattgagaagctgcaacaagagattcgcagccgcagtcttcaacaactggaggaataaggtattaaaggcggaaa acgagttcaaacggcacgtccgaatcgtatcaatggcgagattcgcgcccaggaagttcgcttaactggtctggaaggtgagcagctgggtatt 136 attgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgaacggtagcacagagagcttgctctcgggtgacgagtggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataccgcataacgtcgcaagaccaaagagggggaccttcgggcctctt gccatcagatgtgcccagatgggattagctagtaggtggggtaacggctcacctaggcgacgatccctagctggtctgaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtatgaagaaggccttcgggttgtaa agtactttcagcggggaggaaggcganacggttaataaccgtgttgattgacgttacccgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggtctgtcaagtcggatgtgaaatccccgggctcaacctgggaactgcatccga aactggcaggcttgagtctcgtagagggaggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggcctcctggacgaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtctatttggaggttgtgcccttgaggcg tggcttccggagctaacgcgttaaatagaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctggtcttgacatccacagaacttgccagagatggcttggtgccttcgggaactgtgagacaggtgctgcatgg ctgtcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatccttgttgccagcggtccggccgggaactcaaaggagactgccagtgataaactggaggaaggtggggatgacgtcaagtcatcatggcccttacgaccagggctacacacgtgctacaatggcgcatacaaagagaagcgacc tcgcgagagcaagcggacctcataaagtgcgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtggatcagaatgccacggtgaatacgttcccgggccttgtacacaccgcccgtcacaccatgggagtgggttgcaaagaagtaggtagcttaaccttcgggagggcgctta ccactttgtgattcatgactggggtgaagtcgtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 137 atgaccatgcgtcaatgcgccatttacggcaaaggtgggatcggtaaatcgaccaccacacagaacctggtcgccgcgctggcggagatgggtaagaaagtcatgatcgtcggctgcgatccgaaagccgactccacgcgtttgatcctgcatgcgaaagcgcagaacaccattatggagatggccgccgaagtcggctcc gtcgaagacctggaattagaagacgtgctgcaaatcggttacggcggcgtgcgctgcgcggaatccggtggcccggagccaggtgtgggttgtgccggtcgtggcgtgatcaccgcgattaacttcctcgaagaagaaggcgcttacgtgccggatctggattttgttttctacgacgtgctgggcgacgt ggtatgcggtggtttcgccatgccgattcgtgaaaacaaagcgcaggagatctacatcgtttgctctctggcgaaatgatggcgatgtacgccgccaataacatctccaaaggcatcgtgaaatatgccaaatccggtaaagtgcgcctcggcgggctgatttgtaactcgcgccagaccgaccgcgaagatgaactcatcat tgcgctggcggaaaaactcggcacgcaaatgatccactttgttccccgcgacaacattgtgcagcgtgcggaaatccgccgtatgacggttatcgaatatgacccgacctgcaatcaggccaacgaatatcgcagccttgccagcaaaatcgtcaacaacaccaaaatggtggtaccaaccccctgcaccatggatga actggaagaactgctgatggagttcggcattatggatgtggaagacgccagcatcattggtaaaaccgccgccgaagaaaacgccgtctga 138 atgagcaatgcaacaggcgaacgtaacctggaaatcatcgagcaggtgctggaggttttcccggaaaagacgcgcaaagagcgcagaaaacacatgatggtgacggacccggagcaggagagcgtcggcaagtgcatcatctctaaccgcaaatcgcagccgggcgtgatgaccgtgcgtggctgctcgtat gccggatcaaaaggggtggtatttgggccaatcaaagatatggcgcatatctcccacggcccgatcggctgcgggcagtactcccgcgccgggcggcgtaactactataccggcgtcagcggcgtggacagtttcggcacgctcaacttcacctccgatttccaggagcgcgacatcgtgtttggcggcgacaaaaagct cgccaaactgattgaagagctggaagaactgttccgctgaccaaaggcatttcgattcagtcggaatgcccggtcggcctgattggcgatgatattgaagccgtggcgaacgccagccgcaaagcgatcaacaaaccggttattccggtgcgttgcgaaggctttcgcggcgtgtcgcaatccctcggtcaccatattg ccaacgatgtgatccgcgactgggtactggataaccgcgaaggcaaaccgtttgaatccaccccttacgaatgtggcgatcatcggcgattacaacatcggtggcgacgcctgggcctcgcgcattttgctcgaagatggggttgcgggtggtcgcgcagtggtccggcgacggtacgctggtggagatggaaa acacgccgttcgtcaaactgaacctggtgcactgctaccgctcgatgaactacatctcgcgccatatggaggagaagcacggtattccgtggatggaatacaacttctttggccgacgaaaatcgcggaatcgctgcgcaaaatcgccgacctgttcgacgacaccattcgcgccaacgccgaagcggtgatcgccc gataccaggcgcagaacgacgccattatcgccaaatatcgcccgtctggagggtcgcaaagtgttgctctatatgggcgggctgcgtccgcgccatgtgattggcgcctatgaagatctgggaatggagatcatcgccgccggttatgagtttggtcataacgacgattacgaccgcaccctgccggatctgaaagagggcac gctgctgtttgatgacgccagcagctatgagctggaggcgtttgtcaacgcgctgaaaccggatctcatcggttccggcatcaaagaagaagtacatctttcagaaaatgggcgtgccgttcgccagatgcactcctgggattactccggcccgtaccacggctatgacggcttcgccatcttcgcccgcgatatggatatg acgctcaacaaccccgcctggggtcagttgaccgcgccgtggcttaaatccgcctga 139 atgaaggggaacgacatcctggctctgctcgatgaaccagcctgcgagcataaccataaacagaaaaccggctgtagcgcgccaaaacccggcgccaccgccggaggctgcccttcgacggcgcacagatcaccctgctgccactttccgatgtggcgcatctggtacatggcccgattggctgcgccggcagctcatgggataacc gtggcagcctgagttctggcccgctgattaaccgactcggattcaccactgatttgaacgaacaggatgtcatcatggggcgcggcgagcggcggttgtttcacgcggtgcgccatattgtcgagcgctatcacccggcggcggtatttatttacaacacctgcgttccggctatggaaggcgatgacattgacgcggtct gccaggccgccgcgaccgccaccggtgtgcccgtgattgccgtagatgtggccggttttacggtagcaaaaacctgggtaaccgcctcgcgggcgaggtgatggtgaaaaaagttatcggcgggcgcgaacccgcgccgtggccggacaatacaccttttgccccggcgcaccgccatgacataggcctgattggc gaatttaacatcgccggcgagttctggcatatccagccgctgcttgatgagctgggtattcgcgtccttggctccctttccggcgacgggcgctttgccgagatccagacgttgcaccgcgcgcaggtcaatatgctggtgtgctccagggcgctgattaatgtcgccagatcgcttgaacaacgttatggcacaccctgg tttgaaggcagtttttatggcgttcgcgccacctccgtgccctgcgccagctggcaacactcaccggcgatagcgatttaatggcgcgaaccgaacggctgatcgcacgtgaagagcaagccacagaacaggcgctagcaccgctgcgtgaacggttacacggccggaaagtgctgctctataccggtggcgtgaaatcctgg tcggtggtttcggcgctgcaggatctcggcatgacggtcgttgctaccggaacgcgcaaatccaccgaagaggataaacaacgcatccgtgaactgatgggcgatgacgccatcatgctggatgaaggcaatgcccgcgccttgctggatgtggtctatcgctacaaagccgacatgatgatcgcgggcgggcgcaac atgtacaccgcctataaagcgcgtctgccctttctggatatcaaccaggagcgtgaacacgcgtttgccggttatcgcggcatcatcacgcttgccgaacaactttgtcagacgctggaaagcccggtctggccgcaaacacatgcccgcgccccgtggcaataa 140 atgagccagactgctgagaaaatacagaattgccatcccctgtttgaacaggacgcctaccagacactatttgccggtaaacgggcactcgaagaggctcactcgccggagcgggtgcaggaagtgtttcaatggaccaccaccccggaatacgaagcgctgaacttcaaacgcgaagcgctgactatcgacccggcaaaagcctgccag ccgctgggggcggtgctctgttcgctggggtttgccaacaccctgccgtatgtgcacggttcacagggttgtgtggcctatttccgtacgtactttaaccgccacttcaaagaaccggtggcctgcgtgtcggattcgatgacggaagacgcggccgtgttcggcgggaataacaacctcaaccgggtta caaaacgccagcgcactgtataaaccggagattatcgccgtctctaccacctgtatggcggaagtgatcggtgatgatttacaggcgtttatcgccaacgccaaaaaagatggttttctcgatgccgccatccccgtgccctacgcccacacccccagttttatcggtagccatatcaccggctgggacaacatgtttgaagggtt ttgcccgtacctttaccgcaaaccatcagccacagcccggtaaactttcacgcctgaacctggtgaccgggtttgaaacctatctcggcaatttccgcgtgctgaaacgcatgatggaacaaatggaggtgcaggcgagtgtgctctccgatccgtcggaggtgctggacacccccgccaatggccattaccagatgtacgcg ggcggtacgacgcagcaagagatgcgcgaggcaccggatgccatcgacaccctgctgctgcaaccgtggcagctggtgaaaagcaaaaaagtggtgcaggagatgtggaatcagcccgccaccgaggttgccattcccgtcggctggcaggcacagacgaactgttgatggcgattagccagttaaccggcaaagccattcc cgattcgctggcgctggagcgcgggcggctggtcgatatgatgctcgtcccacacctggttacacggtaaaaaattcggtctgtttggcgatccggattttgtcatgggattgacccgcttcctgctggaactgggctgtgaacctgccgtcatcctctgccataacggtaacaaacgctggcaaaaagcgatga agaaaatgctcgatgcttcaccgtacggccaggagagcgaagtgtttatcaactgcgacttgtggcatttccgctcgctgatgttcacccgccagccggattttatgattggcaactcgtacgccaagtttattcagcgcgacaccttagccaagggcgaacagtttgaagtcccgctgatccgcctcggttttccgctgttcgacc gtcaccatctgcaccgccagaccacctggggctacgagggcgcgatgagcattctcacgacgctggtgaatgcggtactggagaaagtggacaaagagaccatcaagctcggcaaaaccgactacagcttcgatcttatccgttaa 141 atggctgatattgttcgtagtaaaaaaccgctggcggtgagcccgataaaaagcggccagccgctgggggcgatcctggcaagcctgggtttcgaacagtgcataccgctggtacacggcgctcaggggtgcagcgcgttcgcgaaagtgttctttattcaacattttcacgacccgatcccgctgcaatc gacggcgatggacccgacttccaccattatgggcgccgatgaaaacatttttaccgcgctcaatgttctgccagcgcaacgccgcgaaagccatcgtgctgctcagcaccgggctgtcagaagcccagggcagcgatatttcacgagtggtgcgccagtttcgtgatgactttccgcggcataaaaacgtgg cgctgctcaccgtcaacacccggatttctacggctcgctggaaaacggctacagcgccgtgctggaaagcatgattgaacagtgggtgcccgcgcagcccgccgccagcctgcgcaaccgtcgcgtcaacctgctgtcagccatttactgacgccgggcgatatcgaactgttacgcagttatgtggaag cattcggtctgcaaccggtgattgtgccggatctatcgcagtcgctggacggacatctggccaacggtgatttttcgcccgtcacccaggggggaacaccgctgcgcatgattgaacagatggggcaaacctggccactttgtgattggccactcgctggggcgggcggcggcgttactggcgcagcgcagccgtggcga ggtgatcgccctgccgcatctgatgacgcttgatgcgtgcgacacctttatccatcgcctgaaaaccctctccgggcgcgacgtgcccgcgtggattgagcgccagcgcgggcaagtgcaggatgcgatgatcgattgccatatgtggttgcagggcgcggctatcgccatggccgcagaagg cgatcacctggcggcatggtgcgatttcgcccgcagccagggcatgatccccggcccggttgtcgcgccggtcagccagccggggttgcaaaatctgccggttgaaatggtggtcatcggcgatctggaagaagatatgcaggatcggctttgcgcgacgcccgccgcgttactggtggccaattctcatgccgccgatctc gccacgcagtttgatatgtcgcttatccgcccgggtttccggtgtatgaccggctgggggaatttcgtcggctgcgccaggggtatagcggcattcgtgacacgctgtttgagctggcgaatgtgcgcgaacgccattgcccgcttgcaacctaccgctcgccgctgcgtcagcgcttcggc gacaacgttacgccaggagatcggtatgccgcatgttaa 142 atgaccctgaatatgatgatggatgccagcgcgcccgaggccatcgccggtgcgctttcgcaacaacatcctgggctgttttttaccatcgttgaagaagccccgtcgctatttcactaaccgatgccgaggcacgtattgtctatgccaacccggcattctgccgccagaccggctatgagcttgaggagttgttgcagcaa aatccccgcctgcttgccagtcagcagaccccacgggaaatctaccaggatatgtggcacaccctgttacaacgtcgaccatggcgcgggcaattgatcaaccgccaccgtgacggcagccttttctggttgagatcgatatcaccccggtgattaacccgttggcgaactggaacactacctggccatgcagcgcgatatcag cgccggttatgcgctggagcagcggttgcgtaatcacatggcgctgaccgaagcggtgctgaataacattccggcggcggtggtcgtggtcgatgaacgcgatcgtgtggttatggataacctcgcctataaaactttctgtgctgattgcggcggaaaagagctactgagcgaactccatttttcagcccgtaaagc ggagctggcaaacggccaggtcttaccggtggtgctgcgcggcgcggtgcgctggttgtcggtcacctgctgggcgctgccaggcgtcagcgaagaagccagtcgctactttattgataataccttgacgcgcacgctggtggtcatcaccgacgacacccagcagcgccagcagcaagagcaaggacggcttgaccgcc ttaaacagcagatgaccagcggcaaactgctggcggcgatccgcgaagcgcttgacgccgcgctgatccagcttaactgccccatcaatatgctggcggcggcgcggcgtttaaacggcagtgataacagcaacgtagcgctggacgccgcgtggcgcgaaggtgaagaagcgatggcgcggctgaaacggtgccg cccgtcgctggagctggaaaagtgccgccgtctggccgctgcaacccttttttgacgacttgcgcgcgcgcttttcacacccgctacgagcagggtaaaaatttgcaggtcacgctggattcgacgcatctggtgggatttggtcagcgaacccaactgctggcctgcctgagtctgtggctcgatcgcacgct ggatattgccgtcgggctgcgtgatttcaccgcccaaacgcagatttacgcccgggaagaagcgggctggctctcgttgtatatcactgacaatgtgccgttgattccgctgcgccatacccattcgccggatgcgcttaacgcaccgggaaaaggtatggagttgcggctgatccagacgctggtagcgcatcacaacggcg cgatagaactcacttcacgccccgaagggggaagctgcctgaccctacgattcccgctatttcattcactgaccgggaggttcaaaatga 143 atgacccagcgaaccgagtcgggtaataccgtctggcgcttcgatttatcccagcagttcaccgcgatgcagcggataagcgtggttctcagccgggcgaccgaggttgaacagacactccagcaggtgctgtgcgtattgcacaatgacgcctttttgcagcacggcatgatctgtctgtacgcagccagcaggcga ttttgactattgaagcgttgcaggaagccgatcagcagttgatccccggcagctcgcaaattcgctaccgtccgggtgaagggctggtcgggacggtgctttcgcaggggcaatcgttagtgctggcgcgtgtggctgacgatcagcgctttcttgaccgcctgggactgtatgattacaacctgccgtttatc gccgtgccgctgatagggccggatgcgcagacttttggcgtgctgacggcgcaaccgatggcgcgttacgaagagcggttacccgcctgcacccgctttctggaaacggtcgcgaatctggtggcgcagaccgtgcgtttgatgacgccgccggctgcacgcccttccccacgcgctgccatcacgccaac cgccagcccgaaatcgtgcagtacttcacgcgcgttcggcttcgaaaatatggtcggcaacagcccggcaatgcgccagaccatggagattatccgtcaggtttcgcgctgggataccaccgttctggtgcgcggcgagagcggcaccggcaaggaactgattgccaacgccatccatcacaattcgccgcgcgccag tgcgccatttgtgaaattcaactgtgcggcgctgccggacacattgcttgaaagcgaattatttggtcatgaaaaaggcgcctttaccggcgcggtacgccagcgtaaaaggccgttttgagctggccgatggcggcacgctgtttcttgacgaaattggggaaagcagcgcctcgtttcaggctaagctg ctgcgtattttgcaggagggcgaaatggaacgcgtcggtggtgacgagacattgcaagtgaatgtgcgcatcattgccgcgacgaaccgcaaccttgaagatgaagtacgcctgggacattttcgcgaagatctctattaccgcctgaatgtgatgcccatcgccctgccgccgctgcgcgaacgccaggacgacatcg ccgaactggcacattttctggtgcgtaaaatcgcccacaaccagaaccgcacgctgcgcattagcgagggcgctatccgcctgctgatgagctacagctggcccggcaatgtgcgcgaactggaaaactgccttgagcgctctgcggtgatgtcggaaaacggtctgatcgatcgggacgtgattttatttaat catcgcgaccagccagccaaaccgccggttatcagcgtcacgcccgacgataactggctcgataacacccttgacgagcgccagcggctgattgccgcgctggaaaaagcgggatgggtacaagccaaagccgcccgcttgctggggatgacgccgcgccaggtcgcttatcgtattcagaccatggatatcaccctgccaa ggctataa 144 atgccgcaccacgcaggattgtcgcagcactggcaaacggttttttctcgtctgccggaagcgctcaccgcgcaaccattgagcgcgcaggcgcagtcagtgctcacttttagtgattttgttcaggacagcatcatcgtgcatcctgagtggctggcagagcttgaaagcgcaccgccgccagcgaacgagtggca acactacgcgcaatggctgcaagcggcgctggagggcgtcaccgatgaaacctcgctgatgcgcacgctgcggctgtttcgccgtcgcattatggtgcgcatcgcctggagtcaggcgctacagttggtggcggaagaggatatcctgcaacagctcagcgtgctggcggaaactctgatcgtcgccgc gcgcgactggctctatgacgcctgctgccgtgagtggggaacgccgtgcaatccgcaaggcgtcgcgcagccgatgctggtgctcggcatgggcaaacttggcggcggcgaactcaatttctcatccgatatcgatttgatttttgcctggccggaaaatggcaccacgcgcggcggacgccgtgaactggataac gcgcagttttttacccgccttggtcaacggctaattaaagtcctcgaccagcccacgcaggatggctttgtctaccgcgtcgatatgcgcttgcgtccctttggcgacagcggcccgctggtgctgagttttgccgcgctggaagattactaccaggagcaggggcgcgactgggaacgatacgcgatggtgaaagc gcgcattatgggggacaacgacggcgaccatgcgcgagagttgcgcgccatgctgcgcccgttcgttttccgccgctatatcgacttcagcgtgatccagtctctgcgcaacatgaaaggcatgattgcccgcgaagtgcggcgtcgcggcctgaaggacaacataaaactcggcgcgggcggtattc gcgaaatagagtttatcgtgcaggttttccagttgattcgcggcggtcgcgagcctgcgctgcaatcgcgttcgctgttgccgacgcttgctgccattgatcaactacatctgctgccggatggtgatgcaccccggctgcgcgaggcgtattgtggctgcgcggctggaaaacttgctgcaaagcat taatgacgaacagacacagacgctgccggccgatgatttgaatcgcgcgcgcctcgcctggggaatgggcaaagagagctgggaagcgctctgcgaaacgctggaagcgcgcatatgtcggcggtgcggcagattttcaacgatctgattggcgatgatgaaacggattcgccggaagatgcgctttctgagggctggc gcgaattgtggcaggatgcgttgcaggaagaggactctacgccgtgctggcgcatctttccgaggacgatcgccgccgcgtggtggcgctgattgctgattttcgcaaagagctggataaacgcaccattggcccgcgcgggcgacaggtactcgatcacttaatgccgcatctgctcagcgatgtatgctcgcg tgacgatgcgccagtgccgctgtcgcgtctgacgccgctgctcaccggtattattacgcgcaccacttaccttgagctgctgagtgaattccccggtgcgctgaaacacctcatttccctgtgcgccgcgtcgccgatggtggccagccaactggcgcgctacccgatcctgctcgatgaactgctcgacccga acacgctctatcaaccgacggcgatgaacgcctatcgcgatgaactgcgacaatacctgttgcgcgtgccggaagaggatgaagagcagcaactggaggcgctacggcagtttaagcaggcgcagttgttgcgcgtagcggcggcggatatcgccggtacgttacccgtcatgaaagtgagcgatcacttaacctggctgg cggaagcgattatcgatgcggtggtgcagcaagcctggaaccagatggtggcgcgttacggccagccgacgcatctgcacgatcgcgaagggcgcggtttcgccgtggtcggttacggcaaacttggcggctgggaattaggttacagctccgatctggatctggtgttcctgcacgactgccccatggatgtgatgacc gatggcgagcgtgaaatcgatggccgccagttctatttgcgcctcgcgcagcgcgtgatgcacctgttcagcacgcgcacgtcgtccggcattctttatgaagtcgatgcgcgtttgcgcccgtccggcgcggccggaatgctggtgaccactgcggaagcgttcgccgattatcaaa aaaatgaagcctggacatgggagcatcaggcgctggcgcgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatatcctgatgacctcccgcgatgccgctaccctgcaaaccgaagtgcgggaaatgcgtgagaaaatgcgcgcccatcttggtaaca agcacaaagaccgtttcgatctgaaagccgatgaaggcggtatcaccgatattgagtttatcgctcagtatctggtgctgcgctttgcccatgagaagccgaaactgacgcgctggtcggataatgtgcgcatcctcgaagggctggcgcaaaacggcatcatggatgagcaggaagcgcaggcattgacgctggcgtacaccac gttgcgtgatgagctgcaccacctggcgctgcaagagctgccaggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttatcaaaaccagctgggacaagtggctggtggaaccgtgcgccccggcgtaa 145 atgaa a gatgctgacgcaggttgccgtcaccttcgctctggtgtgcatcctgtgggtggtttacggctactctctctggcatttggcgagggcaacagcttcttcggcagtttcaactgggcgatgttgaaaaacatcgaattgaaagccgtgatgggcagcatttatcagtacatccacgtggcgttccagggctcctttgcttgtat caccgttggcctgattgtcggtgcgctggctgagcgtattcgcttctgcggtgctgatttttgtggtggtatggctgacgctttcttatgtgccgattgcgcacatggtctggggtggcggtctgctggcaacccacggcgcgctggatttcgcgggcggtacggttgttcacatcaacgccgcgatcgca ggtctggtggggggcttacctgattggcaaacgcgtgggctttggcaaagaagcgttcaaaccgcataacctgccgatggtcttcaccggcaccgcgatcctctatgttggctggtttggcttcaacgccggctctgcaagctcggcgaacgaaatcgctgcgctggctttcgtgaacacggttgttgccactgcggccgctat tctggcgtgggtatttggcgagtgggcaatgcgcggtaagccgtctctgctcggtgcctgttctggtgccatcgcgggtctggttggtatcaccccggcgtgcggttatgtgggtgtcggcggcgcgctgattgtgggtctgattgccggtctggcagggctgtggggcgttactgcactgaaacg tatgttgcgtgttgatgacccatgcgatgtcttcggtgtgcacggcgtgtgcggcatcgtgggttgtatcctgaccggtatcttcgcgtctacgtcgctgggcggtgtcggtttcgctgaaggggtgaccatgggccatcaggtactggtacagctggaaagcgttgccatcactatcgtg tggtctggcgtggtggcctttatcggttacaaactggcggatatgacggtaggcctgcgcgtaccggaagagcaagagcgtgaagggctggatgtgaacagccacggcgaaaatgcgtataacgcctga 146 ttcttggttctctggagcgctttatcggcatcctgactgaagaatttgcaggcttcttcccaacctggcttgcacccgtgcaggtagttgtgatgaacatcactgattcgcaggctgaatacgttaacgaattgacccgtaaactgcaaaatgcgggcattcgtgtaaaagcagacttgagaaacgagaagattggctttaaaat ccgcgagcacactttacgtcgtgtcccttatatgctggtttgtggtgacaaagaggtcgaagccggcaaagttgctgtgcgtacccgtcgcggtaaagacctgggtagcctggacgtaaatgatgttatcgagaagctgcaacaagagattcgcagccgcagtcttcaacaactggaggaataaggtattaaaggcgg aaaacgagttcaaacggcgcgtcccaatcgtattaatggcgagattcgcgccacggaagttcgcttaacaggtctggaaggcgagcagcttggtatt 147 cgttctgtaataataaccggacaattcggactgattaaaaaagcgccctcgcggcgctttttttatattctcgactccatttaaaataaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgttttaacacgcgtttttaaccttttttattgaaagtcggtgcttctttgagc gaacgatcaaatttaagtggattcccatcaaaaaaatattctcaacctaaaaaagttgtgtaatacttgtaacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgc 148 cgcgtcaggttgaacgtaaaaaagtcggtctgcgcaaagcacgtcgtcgtccgcagttctccaaacgttaattggtttctgcttcggcagaacgattggcgaaaaaacccggtgcgaaccggggttttttatggataaagatcgtgttatccacagcaatccattgattatctcttctttttcagcatttccaga atcccctcaccacaaagcccgcaaaatctggtaaactatcatccaattttctgcccaaatggctgggattgttcattttttgtttgccttacaacgagagtgacagtacgcgcggtagttaactcaacatctgaccggtcgat 149 ttgaagagtttgatcatggctcagattgaacgctggcggcagattgaacgctggcggcaggcctaacacatgcaagtcgngcggaagcacaggagagcttgctctctgggtgacgagcggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataccgcataacgtcgcaagaccaaagagggggaccttcgggcct cttgccatcagatgtgcccagatgggattagctagtaggtggggtaacggcncacctaggcgacgatccctagctggtctgaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtatgaagaaggccttcgggtt gtaaagtactttcagcggggaggaaggtgttgnggttaataaccncagcaattgacgttacccgcagaagaagaagcaccggctaactccgtgccagcagccgcggtaatacggaggntgcaagcgttanncggaatnantgggcgtaaagcgtncgcaggcggtntgtnaagtcggatgtgaaatccccgggctcaacctgg gaactgcattcgaaactggcaggctagagtnnngtagaggngggtagaattccnggtgtagcggtgaaatgcgtagagatcnggangaanaccngtggcgaaggcggcccnctggacaaagactgacgctnaggngcgaaagcgtggggagcaaacaggattagataccctngtagtccacgccgtaaacgatgtcgacttggaggttgtg cccttgaggcgtggcttccggagctaacgcgttaagtcgaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctactcttgacatccagagaacttnncagagatgnnttggtgccttcgggaactctgagacagg tgctgcatggctgtcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatcctttgttgccagcggtncggccgggaactcaaaggagactgccagtgataaactggaggaaggtggggatgacgtcaagtcatcatggcccttacgagtagggctacacacgtgctacaatggcgcataca aagagaagcgacctcgcgagagcaagcggacctcataaagtgcgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtagatcagaatgctacggtgaatacgttcccggccttgtacacaccgcccgtcacaccatgggagtgggttgcaaaagaagtaggtagcttaaccttcggga gggcgcttaccactttgtgattcatgactggggtgaagtcgtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 150 atgaccatgcgtcaatgtgccatttacggcaaaggtggtatcggtaaatccactaccacgcaaaacctggtcgccgcgctggcggagatgggcaagaaagtaatgatcgtcggctgcgacccgaaagcagactccactcgtctgatcctgcatgcgaaagcgcagaacaccattatggagatggcggctgaagtcggct ccgtggaagaccttgaactggaagatgtgctgcaaatcggttacggcgacgtacgctgcgcagaatccggcggcccggaaccaggcgttggctgtgctggtcgcggggtaattaccgccatcaacttcctggaagaagaaggcgcctatgttcccgacctcgatttcgtcttttacgacgtgttgggcgacgtggt gtgcggggggttcgccatgccgattcgcgaaaacaaagcgcaggagatctacatcgtctgctccggcgaaatgatggcgatgtacgccgccaacaacatctctaaaggcatcgtgaaatacgccaaatccggcaaagtgcgccttggcgggctgatctgtaactcccgtcagaccgaccgcgaagatgagctgat catagcgctggcggaaaaactcggcacccagatgatccacttcgtgccgcgcgacaacatcgtgtgcaacgcgctgaaatccgccgtatgacggtgattgagtacgatccgaaatgcaaccaggccaatgaataccgcacgctggcgaacaagatcgtcgtcaacaacaccaaaatggtcgtgccaacgcccatcaccatggac gaactggaagagctgttgatggaattcggcattatggatgtggaagacaccagcattatcggtaaaaccgccgcagaagaaaacgcggtttga 151 atgagcaatgcaacaggcgaacgtaatctggagatcatccaggaagtgctggagatctttccggaaaaaacgcgcaaagaacgcagaaagcacatgatggtgagcgacccggagatggaaagcgtcgggaaatgcatcatctccaaccgtaagtcgcagcccggcgtaatgaccgtgcgcggttgctcttacgccggttc taaaggggtggtattcgggccgatcaaagatatggcccatatttcccacggcccggtcggctgcggtcagtactcccgcgccgggcggcgtaactactaccggcgtcagcggtgtggatagcttcggtacgctcaactttacctccgattttcaggagcgcgatatcgtgttggcggcgataaaaagctgaccaaact gattgaagagatggagacgctgttcccgctgaccaaagggatctccattcagtccgaatgcccggtcggcctgattggcgacgacattgaagccgttgccaacgccagccgcaaagccatcaataaaccggtcattccggtgcgctgcgaaggttttcgcggcgtttcccagtcactcggtcaccacattgccaacgacgtgatccg cgactgggtactggataaccgcgaaggcaagccgtttgaggccggtccttatgacgtggcgatcatcggcgattacaacatcggcggcgatgcctgggcgtcgcgcattttgctcgaagatgggcctgcgcgtggtggcgcagtggtccggcgacggcacgctggttgagatggagaacacgccgttcgtca aactcaaccttgtgcactgctaccgctcaatgaactatatctcccgccatatggaggagaaacacggtattccgtggatggagtacaacttcttcggtccgaccaaagtcgccgaatcgttgcgcaaaatcgccgatatgtttgatgacaccattcgcgccaacgccgaagcggtgatcgccaaatatcaggcgcagaacgacg ccatcatcgccaaataccgtccgcgtctggaaggccgcaaagtgctgctgtatatgggcggtttacgtcctcgccatgtgattggcgcttatgaagatctggggatggaaattatcgctgcggttatgaattcgcccacaacgatgactacgaccgcaccctgccggatctgaaagaaggcaccttgctgttcgacgat gccagcagttatgaactggaagcctttgtcaaagcgctgaagccggatctgatcggctccggcattaaagagaagtacatcttccagaaaatgggcgtgccgtttcgccagatgcactcctgggattactccggcccctatcacggttatgacggctttgccatcttcgcccgcgatatggatatgacgatcaacaaccccgcgtgg ggccagttgaccgcgccgtggctgaaatccgcctga 152 atgggacgcggcgagcgccgcctgttccatgccgtgcgccacatcgtcaaccgctaccacccggccgccgtcttttatctataacacctgcgttcccgcgatggagggcgacgatatcgaagccgtctgccaggcggcagaaaccgccatcggcgtaccggtgattgccgttgatgtcgccgggttttacggca gcaaaaatctcggcaaccggttggccggtgaagtgatggtgaaaaaggtgattggcgggcgtgaacccgcgccgtggccgggaagataccccttttgccccggcgcaccgccacgatcgggctgattggcgaattcaatattgccggagagttctggcatattcagccgctgctcgatgagctggggtattcgcgtgct cggcagcctctccggcgacgggcgcttcagtgaaatccagacgctgcaccgggcgcaggtcaatatgctggtctgctccagggcgctgatcaacgtcgcccgctcgctggagcagcgctacggcacgccgtggtttgaaggcagtttttatggtgttcgcgccacctctgacgccctgcgccaactggcggc gctgaccggagaccgcgatctgatgcagcgcaccgaacagctcattgcccgcgaagagcagcaaacagagcaggcgctggccccgctgcgcgagcgcctgcgcgggcgcaaagcgctgctctatnccggcggcgtgaaatcctggtcggtggtttcggcgcttcaggatctgggcatggaagtggtggcga ccggcacgcgcaaatccaccgaagaggataaacagcgcatccgcgaactgatgggcgccgacgcgctgatgcttgatgaaggtaacgcccgctcgctgctggacgtggtttaccgctacaaggcggacatgatgatcgccgggggacgcaatatgtacaccgcctacaaagcgcggctgccgttcctcgatatcaatca ggagcgcgagcacgcctttgccggctaccgcggcattgtcaccctggccgaacagctgcctgaccatggaaagcccggtctggccgcaaacccattcccgcgcaccgtggcaataa 153 atgatggagcaaatggacgtgccgtgcagcctgctttccgatccctccgaagtgctggataccccggctgacgggcattaccacatgtatgcgggcggtacgacccagcaggagatgcgcgaagcgcctgacgctatcgacaccctgctgctgcaaccctggcaactggtgaaaaccaaaaaagtggtgcaggaaagct ggaaccagcccgctaccgaggtgcaaatcccaatggggctggccggaaccgacgagctgctgatgacggtaagccagttaaccggcaaagccattccggatagcttagcgctggaacgcggtcggctggtggatatgatgctcgactcccacacctggctgcacggcaagaaattcggcctgttcggtgacccggattttgtcatgg ggctgacccgcttcctgctggaactgggctgcgaaccgacggtgattctgtgccataacggcagcaagcgctggcagaaagcgatgaagaaaatgcttgaagcctcgccgtacgggaaagagcgaagtctttatcaactgcgatttgtggcatttccgctcgctgatgtttacccgtcagccggactttatgatcggcaact cctacgccaagtttatccagcgcgatacgctggcgaagggtgagcagtttgaagtgccgctgatccgcctggggttcccgctgttcgatcgccaccatctgcaccgccagaccacctggggttacgaaggggccatgagtatcctcaccacgctggttaatgcggtgctggagaaagtcgacagagagaccatcaagctcggcaaa accgactacagcttcgatcttatccgttaa 154 atgcagcgcgacatcagcaccagctacgcgctggaacaacggctgcgcaatcatatgacgctgaccgaagccgtcttgaataacattccggcggcggttgtagtggtggatgaacgcgcgatcgggtggtgatggataacctcgcctacaaaaccttttgcgccgattgcggcggtaaagaactactcaccgaaatcaacttttccg cccataaggcggagctggcgcagggcctggtactgccggtagtgctgcgcggcaccgtgcgctggttgtccgttacctgttgggcgctgccgggcgtcagcgaagaagcaggccgctactttattgatagcgccgtgccgcgcacgctggtggtgatcaccgataatactcagcagcagcaacaacaggagcaggggc gtcttgatcgtctgaagcagcagataaccagcggtaaattgctggcggcgatccgcgaatcgctggacgccgcgctggtacaactcaattgcccaattaatatgctggccgccgcacgccgcttaaatggcgacgagcatagcaatctggcgctggatgccgcatggcgtgaaggcgaagaagcgatggcgcggttgcag cgctgccgcccgtcgctggaactggaaagcccggcagtctggccgctccagccgttccttgacgatctgcgtgccctgtatcacacccgatataaccagggcgaaaacctgcaaattgagctggaatcccccgacctggtgggctttggccagcgaacacaactgcttgcctgcctgagcctgtggctcgacaga accctggatattgccgcggagctacgtgatttcacggtacagactcaactttacgcccgcgaagagcggctggctgtcgttctatttaaacgacaatgtgccgctgattcaggtgcgctacacccattcacccgatgcactnaatgccccggtaaaggcatggagctgcggctgatccagacgctggtcgcccaccatcgagg cgcaatagaactgacctcacgccctcagggaggcacctgtctgatcctgcgtttcccattattttactcgctgacaggaggctcactatga 155 atgactcagcgaaccgagtcgggtacaaccgtctggcgctttgacctctcccaacagtttacagccatgcagcgtatcagtgtggtgttaagccgcgcgacggagatcgggcagacgctacaggaagtgctgtgcgtgctgcacaacgatgcctttatgcagcacgggatgatctgtccgtacgc gcgggtgcgcgtcttcgcgagcgtatggctttga 156 atgcgcgtggaagactggtcaacgctgaccgaacggctcgatgcccatatggcaggcgtgcgccgaatctttaacgaactgatcggtgatgacgaaagtgagtcgcaggacgatgcgctctccgagcactggcgcgagctgtggcaggacgcgcttcaggaagatgacaccacgccggtgctgacgcacttaacc gacgacgcgcgccatcgcgtggtggcgctgatcgctganttccgtcttgagctgaacaaacgcgccatcggcccgcgtngtcgccaggtgctggatcacctgatgccgcacctgctgagcgaagtctgctcgcgtgccgatgcgccggtgccgctgtcgcggatgatgcccctgctga gcgggattatcacccgtactacctaccttgaactcctgagcgagttccctggcgcgcttaagcacctgatttcactctgcgccgcgtcgccgatggtggccaacaagctggcgcgttacccgctgctgctggatgagctgctcgatccgaataccctttatcaaccgacggcgaccgacgcctaccgggacgaactgcgtca gtatctgctgcgcgtgccggaagaagacgaagagcaacagctggaggcgctgcgtcagtttaagcaggcccagatgctgcgcgtggcggccgcagatattgccggaacgctgccggtgatgaaagtgagcgatcacttaacctggcttgcggaagcgattatcgacgcggtggtgcatcaggcctgggtgca gatggtggcgcgctatggccagccgaaacatctggctgaccgtgatggtcgcggcttcgcggtggtgggttacggtaagctcggcggttgggagctgggctatagctccgatctggatttaatcttcctccacgactgcccggttgatgtgatgaccgacggcgagcgcgagattgacgggcgtcagttctacctgc gcctggcgcagcgcatcatgcacctgttcagcacccgcacctcgtcgggcattttgtatgaagtggatgcccgtctgcgcccgtccggcgcggcgggcatgctggtcacctcgacggagtccttcgctgattaccagaagaatgaagcctggacgtgggagcatcaggcgctggtgcgcgcccgtgtggt gtatggcgatccgctgctgaaaacgcagtttgacgtgattcgtaaggaagtcatgaccaccgtgcgcgatggcagcacgctgcaaacggaagtgcgcgaaatgcgcgagaaaatgcgcgcgcacttaggcaataaacatcgcgatcgctttgatattaaagccgatgagggcggtattaccgatattgag tttattacccagtatctggtgttgctgcacgcgcatgacaagccgaagctgacgcgctggtcggataacgtgcgcattctggaactgctggcgcaaaacgacattatggacgagcaggaggcgcaggccttaacccgtgcctatacaacgcttcgcgatgagctccatcatctggcgttgcaggagcagcngggacacgtgg cgctggactgtttcaccgctgaacgcgctcaggtaacggccagctggcagaagtggctggtggaaccgtgcgtaacaaatcaagtgtga 157 agatgtgcccagatgggattagctagtaggtggggtaacggcncacctaggcgacgatccctagctggtctgagaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtatgaagaaggccttcgggttgtaaag tactttcagcggggaggaaggtgttgtggttaataaccncagcaattgacgttacccgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggtctgtcaagtcggatgtgaaatccccggggctcaacctgggaactgcatt cgaaactggcaggctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtcgacttggaggttgtgcccttgagg cgtggcttccggagctaacgcgttaagtcgaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctactcttgacatccagagaacttnncagagatgnnttggtgccttcgggaactctgagacaggtgctgcat ggctgtcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatccttgttgccagcggtccggccgggaactcaaaggagactgccagtgataaactggaggaaggtggggatgacgtcaagtcatcatggcccttacgagtagggctacacacgtgctacaatggcgcatacaaagagaagcga cctcgcgagagcaagcggacctcataaagtgcgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtagatcagaatgctacggtgaatacgttccgggccttgtacacaccgcccgtcacaccatgggagtgggttgcaaaagaagtaggtagcttaaccttcgggagggcgctta ccactttgtgattcatgactggggtgaagtcgtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 158 atgaccatgcgtcaatgtgccatttacggcaaaggtggtatcggtaaatccactaccacgcaaaacctggtcgccgcgctggcggagatgggcaagaaagtaatgatcgtcggctgcgacccgaaagcagactccactcgtctgatcctgcatgcgaaagcgcagaacaccattatggagatggcggctgaagtcggct ccgtggaagaccttgaactggaagatgtgctgcaaatcggttacggcgacgtacgctgcgcagaatccggcggcccggaaccaggcgttggctgtgctggtcgcggggtaattaccgccatcaacttcctggaagaagaaggcgcctatgttcccgacctcgatttcgtcttttacgacgtgttgggcgacgtggt gtgcggggggttcgccatgccgattcgcgaaaacaaagcgcaggagatctacatcgtctgctccggcgaaatgatggcgatgtacgccgccaacaacatctctaaaggcatcgtgaaatacgccaaatccggcaaagtgcgccttggcgggctgatctgtaactcccgtcagaccgaccgcgaagatgagctgat catagcgctggcggaaaaactcggcacccagatgatccacttcgtgccgcgcgacaacatcgtgtgcaacgcgctgaaatccgccgtatgacggtgattgagtacgatccgaaatgcaaccaggccaatgaataccgcacgctggcgaacaagatcgtcgtcaacaacaccaaaatggtcgtgccaacgcccatcaccatggac gaactggaagagctgttgatggaattcggcattatggatgtggaagacaccagcattatcggtaaaaccgccgcagaagaaaacgcggtttga 159 atgagcaatgcaacaggcgaacgtaatctggagatcatccaggaagtgctggagatctttccggaaaaaacgcgcaaagaacgcagaaagcacatgatggtgagcgacccggagatggaaagcgtcgggaaatgcatcatctccaaccgtaagtcgcagcccggcgtaatgaccgtgcgcggttgctcttacgccggttc taaaggggtggtattcgggccgatcaaagatatggcccatatttcccacggcccggtcggctgcggtcagtactcccgcgccgggcggcgtaactactaccggcgtcagcggtgtggatagcttcggtacgctcaactttacctccgattttcaggagcgcgatatcgtgttggcggcgataaaaagctgaccaaact gattgaagagatggagacgctgttcccgctgaccaaagggatctccattcagtccgaatgcccggtcggcctgattggcgacgacattgaagccgttgccaacgccagccgcaaagccatcaataaaccggtcattccggtgcgctgcgaaggttttcgcggcgtttcccagtcactcggtcaccacattgccaacgacgtgatccg cgactgggtactggataaccgcgaaggcaagccgtttgaggccggtccttatgacgtggcgatcatcggcgattacaacatcggcggcgatgcctgggcgtcgcgcattttgctcgaagatgggcctgcgcgtggtggcgcagtggtccggcgacggcacgctggttgagatggagaacacgccgttcgtca aactcaaccttgtgcactgctaccgctcaatgaactatatctcccgccatatggaggagaaacacggtattccgtggatggagtacaacttcttcggtccgaccaaagtcgccgaatcgttgcgcaaaatcgccgatatgtttgatgacaccattcgcgccaacgccgaagcggtgatcgccaaatatcaggcgcagaacgacg ccatcatcgccaaataccgtccgcgtctggaaggccgcaaagtgctgctgtatatgggcggtttacgtcctcgccatgtgattggcgcttatgaagatctggggatggaaattatcgctgcggttatgaattcgcccacaacgatgactacgaccgcaccctgccggatctgaaagaaggcaccttgctgttcgacgat gccagcagttatgaactggaagcctttgtcaaagcgctgaagccggatctgatcggctccggcattaaagagaagtacatcttccagaaaatgggcgtgccgtttcgccagatgcactcctgggattactccggcccctatcacggttatgacggctttgccatcttcgcccgcgatatggatatgacgatcaacaaccccgcgtgg ggccagttgaccgcgccgtggctgaaatccgcctga 160 atgaaggggaacgagatcctggctttgctcgatgaacctgcctgcgagcacaaccataaacagaaatccggctgcagcgcgccgaaacccggcgcgacagcggggcggctgcgcctttgacggtgcgcagatcaccctgctgccactctccgatgttgcccacctggtacacggccccattggttgtaccggtagctcatgggata accgtggcagcttcagttccggcccgacgatcaaccggctgggttttaccaccgatctgagcgaacaggatgtgatcatgggacgcggcgagcgccgcctgttccatgccgtgcgccacatcgtcaaccgctaccacccggccgccgtctttatctataacacctgcgttcccgcgatggagggcgacgatatcga agccgtctgccaggcggcagaaaccgccatcggcgtaccggtgattgccgttgatgtcgccgggttttacggcagcaaaaatctcggcaaccggttggccggtgaagtgatggtgaaaaaggtgattggcgggcgtgaacccgcgccgtggccggaagataccccttttgccccggcgcaccgccacgatatcgggg ctgattggcgaattcaatattgccggagagttctggcatattcagccgctgctcgatgagctgggtattcgcgtgctcggcagcctccggcgacgggcgcttcagtgaaatccagacgctgcaccgggcgcaggtcaatatgctggtctgctccagggcgctgatcaacgtcgcccgctcgctggagcagcgct acggcacgccgtggtttgaaggcagtttttatggtgttcgcgccacctctgacgccctgcgccaactggcggcgctgaccggagaccgcgatctgatgcagcgcaccgaacagctcattgccccgaagagcagcaaacagagcaggcgctggccccgctgcgcgagcgcctgcgcgggcgcaaagcgctg ctctataccggcggcgtgaaatcctggtcggtggtttcggcgcttcaggatctgggcatggaagtggtggcgaccggcacgcgcaaatccaccgaagaggataaacagcgcatccgcgaactgatgggcgccgacgcgctgatgcttgatgaaggtaacgcccgctcgctgctggacgtggtttaccgctacaaggcgg acatgatgatcgccgggggacgcaatatgtacaccgcctacaaagcgcggctgccgttcctcgatatcaatcaggagcgcgagcacgcctttgccggctaccgcggcattgtcaccctggccgaacagctctgcctgaccatggaaagcccggtctggccgcaaacccattcccgcgcaccgtggcaataa 161 atgagccaaagtgctgagaaaattcaaaactgtcatccgctgtttgaacaggatgcgtaccagatgctgttgtttaaagataaacggcaactggaagaggcccacgatccggcgcgcgtgcaggaggtctttcaatggaccaccaccgccgagtatgaagcgcttaactttcaacgcgaagcgctgactatcgatccggccaaagcct gccagccgctgggtgcggtactgtgctcgctgggctttgccaataccctgccctatgttcacggctcccaggggtgcgtggcctatttccgcacctattttaaccgtcactttaaagagccgattgcctgtgtttctgactcgatgacggaagatgcggcagtattcggcggcaacaacaacctgaacaccggggttgca gaacgccagcgccctctacaagccggaaatcattgccgtctccaccacctgtatggcgggaggtcatcggcgacgacctgcaggcgtttattgctaacgccaaaaaagacggcttttcgacgcggcgatcccggtgccttacgcgcacacgccaagctttatcggcagccatatcaccggctgggacaatgtttgagggct tcgcccgtacctttaccgccgattacagcggacaaccgggcaaattaccgcgtatcaatctggtcagcggatttgaaacctatctcggtaatttccgcgtgctgaaacgcatgatggagcaaatggacgtgccgtgcagcctgctttccgatccctccgaagtgctggataccccggctgacgggcattaccacatgtatgcggg cggtacgacccagcaggagatgcgcgaagcgcctgacgctatcgacaccctgctgctgcaaccctggcaactggtgaaaaccaaaaaagtggtgcaggaaagctggaaccagcccgctaccgaggtgcaaatcccaatggggctggccggaaccgacgctgctgatgacggtaagccagttaaccggcaaagccattcc ggatagcttagcgctggaacgcggtcggctggtggatatgatgctcgactcccacacctggctgcacggcaagaaattcggcctgttcggtgacccggatttgtcatggggctgacccgcttcctgctggaactgggctgcgaaccgacggtgattctgtgccataacggcagcaagcgctggcagaaagcgatgaagaaaat gcttgaagcctcgccgtacgggaaagagagcgaagtcttttcaactgcgatttgtggcatttccgctcgctgatgtttacccgtcagccggactttatgatcggcaactcctacgccaagtttatccagcgcgatacgctggcgaagggtgagcagtttgaagtgccgctgatccgcctggggttccgctgttcgatc gccaccatctgcaccgccagaccacctggggttacgaaggggccatgagtatcctcaccacgctggttaatgcggtgctggagaaagtcgacagagagaccatcaagctcggcaaaaccgactacagcttcgatcttatccgttaa 162 atggcagaaattatccgtagtaaaaagccgctggccgtcagcccggtaaaaagtggccagccgctgggcgcgattctggcgagcatgggctttgaacagagcattccgctggttcatggcgctcaggggtgcagcgccttcgcgaaggtcttttttatccagcattttcacgatccgatcccgctgcaatcgacggcaatggac ccgacatcgaccattatgggtgccgatgagaacatcttttaccgcgctgaatgtgctgtgttcacgcaacaacccgaaagcgattgttctgctgagcactggcctttccgaggcgcagggcagcgatatttcgcgcgtggtgcgccagttccgcgatgaatatccgcgccataaaggggtggcgctgctgaccgt caacacgccggatttttacggcagcctggaaaacggctacagcgcggtgctggagagcatggttgaacagtgggtgccggaaaaaccgcagccgggcgtgcgcaatcgccgcgtgaacctgctgctcagccatttgcttacgccgggcgacattgagctgctgcgaagttatgtcgaggcatttggcctgcagcc ggtgatggtgccggatctttcccagtcgctggatggccatctcgccagcggggatttctcgccaattacccagggcggcagcagcctgcggctgattgaacagatgggacagagtcttggcacgttcgccattggcgtatccctctcccgcgccgcgcaattgctggcgcagcgcagccatgcggaagtggtcaccctg ccgcatctgatgaccatgagccagtgcgatacgtttattcatcaactgaagcgcctctccgggcgcgatgttccggcgtggatcgaacgccagcgcgggcaactgcaggatgcgatgatcgattgtcatatgtggttgcagggcgcgcctgtcgcgctggccgccgagggcgatctgctcgccgcctggtg cgatttcgcctgcgatatgggcatggtgcccggcccggtggtggcgccggtgagccagaaagggttgcaggatctgccggtcgaaaaagtcattatcggcgatctggaggatatgcaggatctgttgtgtgaaacgcctgcatcgctgctcgtctctaattctcacgccgctgatttggccgggcagttcga cattccgctggtgcgcgccggtttccccctgttcgaccgtctgggcgagtttcgccgcgtgcgccagggttacgccgggatgcgcgacaccttgtttgagctggcgaatgcgctgcgcgatcgccatcatcatcttgccgcttatcactcgccgctgcgccagcgtttttacgaacccgcatcttcgg gaggtgactatgcaacatgttaa 163 atgaccctgaatatgatgatggacgccaccgcgcccgccgagatcgccggagcgctctcacaacagcatcccgggttgttttcaccatggttgaacaggcgcccgtcgcgatttcactgaccgatgccgatgcccacattctctacgccaaccccgcgttttgtcgccagtcggggtatgaactggaagagttgttgcag caaaacccgcgcctgcttgccagtaagcagacgccgcgtgaaatctaccaggaaatgtggcacaccctgctgcaacaccgtccgtggcgcggacaactgatcaaccgtcgccgcgacggcagcctgtttctggtggaaatcgacatcaccccactgtttgatgcgttcggcaaactcgaacattacctggccat gcagcgcgacatcagcaccagctacgcgctggaacaacggctgcgcaatcatatgacgctgaccgaagccgtcttgaataacattccggcggcggttgtagtggtggatgaacgcgatcgggtggtgatggataacctcgcctacaaaaccttttgcgccgattgcggcggtaaagaactactcaccgaaatcaacttttccgccca taaggcggagctggcgcagggcctggtactgccggtagtgctgcgcggcaccgtgcgctggttgtccgttacctgttgggcgctgccgggcgtcagcgaagaagcaggccgctactttattgatagcgccgtgccgcgcacgctggtggtgatcaccgataatactcagcagcagcaacaacaggagcaggggcgt cttgatcgtctgaagcagcagataaccagcggtaaattgctggcggcgatccgcgaatcgctggacgccgcgctggtacaactcaattgcccaattaatatgctggccgccgcacgccgcttaaatggcgacgagcatagcaatctggcgctggatgccgcatggcgtgaaggcgaagaagcgatggcgcggttgcagcg ctgccgcccgtcgctggaactggaaagcccggcagtctggccgctccagccgttccttgacgatctgcgtgccctgtatcacacccgatataaccagggcgaaaacctgcaaattgagctggaatccccgacctggtgggctttggccagcgaacacaactgcttgcctgcctgagcctgtggctcgacagaaccc tggatattgccgcggagctacgtgatttcacggtacagactcaactttacgcccgcgaagagcggctggctgtcgttctatttaaacgacaatgtgccgctgattcaggtgcgctacacccattcacccgatgcactcaatgcgcccggtaaaggcatggagctgcggctgatccagacgctggtcgcccaccatcgaggcg caatagaactgacctcacgccctcagggaggcacctgtctgatcctgcgtttcccattattttactcgctgacaggaggctcactatga 164 atgactcagcgaaccgagtcgggtacaaccgtctggcgctttgacctctcccaacagtttacagccatgcagcgtatcagtgtggtgttaagccgcgcgacggagatcgggcagacgctacaggaagtgctgtgcgtgctgcacaacgatgcctttatgcagcacgggatgatctgtctgtacgaca gtaagcaagcgatcctttccattgaagccttgcatgaggccgatcagcagttaattcccggcagttcacagattcgctaccgtccgggcgaagggctggtaggcacggtgctttcacagggacagtcgctggtactgccctgtgtctccgacgatcggcgttttctcgatcgcctgggattgtatgattacagcttgccgtt tatcgccgtgccgctgatggggccaaactcgcagcctatcggcgtgctggccgcccagcctatggcgcgttacgaggagcggctgcccgcctgcacgcgttttcttgaaaccgtcgccaatctggtggcgcaaaccgttcgcctgatgacaccgcccagcgtcgcgtctccaccccgtgctgct gccgcgcagattgccagccagcgcgggtgcgcgtcttcgcgagcgtatggctttgaaaacatggtcggtaaaagcgcggctatgcgtcagacgctggaaattattcgccaggtatcacgctgggacaccaccgtgctggtgcgtggcgaaagcggaaccggtaaagagttgatagccaacgctatccaccaca attcaccgcgcgccgccgcgccgtttgtcaaattcaactgcgcggcgctgcccgatacgctgctggagagtgaactcttcggtcatgaaaaaggcgcgtttaccggcgcggtgcgccagcgcaaaggccgttcgaactggcggatggcggtacgctgtttcttgatgagatcggcgaaagtagc gcctcgtttcaggcgaaattgctgcgtatcttgcaggaaggcgaaatggaacgcgtcggcggcgacgaaacgctgcgggtgaatgtacggatcattgccgccaccaaccgcaatctggaagaggaagtgcggctgggtaattttcgcgaagatctctactatcgccttaatgtgatgccgatctccctgcc cccgctccgcgagcgtcaggaggacatcgtcgagctggcgcattttctggtgcgcaaaatcgcgcaaaaccagaaccgcacgctgcgcatcagcgatggcgcgatccgtttgttgatgagctatagctggcctggaaacgtgcgtgagctggaaaactgccttgagcgatcggcggtgatg tcggaaaacgggctgatcgatcgcggctgatcgatcgcggctgatcgatcgcgacgtgattttgtttcaccacagggaaaatctgccaaaaacgccacagaccagtgcgccgcgcgaagagagctggctcgatcagaacctcgatgagcgacaaagattgatcgccgcgctggagaaagccggttgggtacaggcaaaagccgcgcgcctgctgggaatgacc ccgcgccaggtggcctatcgtattcagacgatggacattgccatgccgagattgtag 165 atgccgctttcttcgcagttacagcagcagtggcagaccgtttgcgaacgtctgcctgagtcattaccggcgtcatcgttaagcgagcaggcaaagagcgtgctcgtcttcagtgattttgtgcaggaaagtatcaccgccaacccgaactggctggcggaacttgagaacgcaccaccgcaggcagaagagtggcgg cactatgctggctggctgcaaactgtactcgaagacgttacggatgaggccacgctgatgcgcgtcctgcgccagttccgtcgtcgggtgatggtgcgcattgcctgggctcaggcgctggaactggtgagcgaagagagtacgctgcagcagttaagcgagctggcgcaaacgttgattgtcgccgcg cgagactggctctatgccgcctgctgtaaagagtggggcacgccgtgcagcgaggaaggggttcctcagccgctgttgattctggggatgggaaagctgggcggctgcgagctgaacttctcctctgatatcgacctgatttttgcctggccggagaacggctccacgcgcggaggccgccgcgagctggacaacgcgca gttctttacccgtctcggccagcgcctgattaaagcgctggatcagcccacgcaggacggttttgtttaccgcgtggacatgcgcctgcgtccgtttggcgacagcgggccgctggtgctgagctttgcggcgctggaagattattaccaggagcaaggtcgcgactgggagcgttacgcgatggtcaaagcgc ggatcatgggcgacagcgacgacgcttatgccaacgagctgcgcgccatgctgcgtccgttcgtgttccgtcgctatatcgacttcagcgtcatccagtccctgcgaaatatgaaagggatgattgcccgcgaggtgcgccgccgtgggctgaaagacaatatcaagctcggtgcgggcggcatccgcga aatcgaatttatcgtccaggtcttccagcttattcgcggcggacgcgagccgtcgctgcagtcccgttccttattaccgacgctgagcgccattgcgcagctgcatctcctgccggacggcgacgcgcaaaccctgcgcgaggcctatcttttcctgcgtcgtctggaaaacctgctgcaaagcattaat gacgaacagacccaaaccctgccgggcgacgaccttaaccgggcgcgtctggcctggggaatgcgcgtggaagactggtcaacgctgaccgaacggctcgatgcccatatggcaggcgtgcgccgaatctttaacgaactgatcggtgatgacgaaagtgagtcgcaggacgatgcgctctccgagcactggcgc gagctgtggcaggacgcgcttcaggaagatgacaccacgccggtgctgacgcacttaaccgacgacgcgcgccatcgcgtggtggcgctgatcgctgatttccgtcttgagctgaacaaacgcgccatcggcccgcgtggtcgccaggtgctggatcacctgatgccgcacctgctgagcgaagt ctgctcgcgtgccgatgcgccggtgccgctgtcgcggatgatgcccctgctgagcgggattatcacccgtactacctaccttgaactcctgagcgagttccctggcgcgcttaagcacctgatttcactctgcgccgcgtcgccgatggtggccaacaagctggcgcgttacccgctgctgctggatgag ctgctcgatccgaatacccttttcaaccgacggcgaccgacgcctaccgggacgaactgcgtcagtatctgctgcgcgtgccggaagaagacgaagagcaacagctggaggcgctgcgtcagtttaagcaggcccagatgctgcgcgtggcggccgcagatattgccggaacgctgccggtgatgaaagtgagcga tcacttaacctggcttgcggaagcgattatcgacgcggtggtgcatcaggcctgggtgcagatggtggcgcgctatggccagccgaaacatctggctgaccgtgatggtcgcggcttcgcggtggtgggttacggtaagctcggcggttgggagctgggctatagctccgatctggatttaatcttcctccacgactgc ccggttgatgtgatgaccgacggcgagcgcgagattgacgggcgtcagttctacctgcgcctggcgcagcgcatcatgcacctgttcagcacccgcacctcgtcgggcattttgtatgaagtggatgcccgtctgcgcccgtccggcgcggcgggcatgctggtcacctcgacggagtccttcgct gattaccagaagaatgaagcctggacgtgggagcatcaggcgctggtgcgcgcccgtgtggtgtatggcgatccgctgctgaaaacgcagtttgacgtgattcgtaaggaagtcatgaccaccgtgcgcgatggcagcacgctgcaaacggaagtgcgcgaaatgcgcgagaaaaatgcgcgcgc acttaggcaataaacatcgcgatcgctttgatattaaagccgatgagggcggtattaccgatattgagtttattacccagtatctggtgttgctgcacgcgcatgacaagccgaagctgacgcgctggtcggataacgtgcgcattctggaactgctggcgcaaaacgacattatggacgagcaggaggcgcaggccttaacccgtg cctatacaacgcttcgcgatgagctccatcatctggcgttgcaggagcagccgggacacgtggcgctggactgtttcaccgctgaacgcgctcaggtaacggccagctggcagaagtggctggtggaaccgtgcgtaacaaatcaagtgtga 166 atgaagatagcaacacttaaaacgggtctgggttcgctggcactgctgccgggcctggcgctggctgctgcacctgcggtggcagacaaagccgataacgcctttatgatgatcagcaccgcgctggtgctgttcatgtccattccgggcattgcgctgttctatggcggcctgatccgtggcaaaaacgttctctctccatg ctgacgcaggttgccgtaacgttcgcgctggtctgcgtactgtgggtggtttacggttactcgctggctttcggcacgggcaacgcgttctttggtaacttcgactgggtgatgctgaaaaatattgaactgaccgcgctgatgggcagtttctaccagtatattcacgttgctttccagggctcgttcgcctgcatta ccgtcgggctgattgtaggcgcgcttgccgagcgtattcgtttctctgcggtcctgatcttcgtggtggtctggctgacgctctcctatgtgccgattgcgcacatggtctggggtggcggtctgctggcgacgcatggcgcgctggacttcgcgggcggtaccgttgtgcacattaacgccgcggtag cgggtctggttggcgcatacctgattggcaaacgcgtgggcttcggtaaagaagcgttcaaaccgcacaacctgccgatggtcttcaccggtaccgcgatcctctactttggctggtttggtttcaacgccggctcagcaagtgccgcgaacgaaatcgccgcgctggccttcgtgaataccgttgtggccacggcaggtg caatcctctcctgggtctttggcgagtgggctgtgcgcggtaaaccttctgctgggtgcctgttcgggggcgattgctggtctggtcggtatcaccccagcatgtggttatgtcggtgtgggtggcgcgctgctggtcggcctggtgtcaggtctggcgggtctgtggggcgtgacggcgct gaaacgtattctgcgcgttgatgacccttgcgatgtgtttggcgtgcacggcgtgtgcggcatcgtcggctgtatcatgaccggtatctttgcagcgaaatcgctgggtggcgtgggctacgcagaaggcgtcaccatggcccatcaggtgctggtgcagctggaaagtattgctgtcaccgtgg tgtggtctgccgttgtcgctttcattggctacaaactggcggacatgacggttggtctgcgcgtgccggaagagcaggaacgcgaaggtctggacgtcaacagccacggcgagaatgcgtataacgcatga 167 gccgagagaggggcccgcgtcggattaggtagttggtgaggtaatggctcaccaagccttcgatccgtagctggtctgagaggatgatcagccacactgggactgagacacggcccagactcctacgggaggcagcagtggggaatattggacaatgggcgcaagcctgatccagcaatgccgcgtgagtgatgaaggccttagggttgtaaag ctctttcgcacgcgacgatgatgacggtagcgtgagaagaagccccggctaacttcgtgccagcagccgcggtaatacgnagggngcnagcgttnntcggaattactgggcgtaaagngcgcgtaggcggcntgttnagtcagaagtgaaagccccgggctcaacctgggaatagcttttgatactggcaggcttgagttccgg agaggatggtggaattcccngtgtagnggtgaaatncgtagatattgggangaacaccngtggcgaaggcggcnatctggacgganactgacgctgaggcgcgaaagcgtggggagcaaacaggattagataccctngtagtccacgccgtaaacgatgaatgctagacgtcggggtgcatgcacttcggtgtcgccgctaacg cattaagcattccgcctggggagtacggccgcaaggttaaaactcaaaggaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgaagcaacgcgcagaaccttaccaacccttgacatgtccactttgggctcgagagatngggtccttcagttcggctgggtggaacacaggtgctgcatggctgtcgtcagctc gtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccctaccgtcagttgccatcattcagttgggcactctggtggaaccgccggtgacaagccggaggaaggcggggatgacgtcaagtcctcatggcccttatgggttgggctacacacgtgctacaatggcggtgacagtgggaagcgaagtcgc gagatggagcaaatccccaaaagccgtctcagttcggatcgcactctgcaactcgagtgcgtgaagttggaatcgctagtaatcgcggatcagcacgccgcggtgaatacgttcccgggccttgtacacaccgcccgtcacaccatgggagttggttttacccgaaggtggtgcgctaaccgcaaggaggcagccaacc acggtaaggtcagcgactggggtgaagtcgtaacaaggtagccgtaggggaacctgcggctggatcacctccttt 168 atggccaaagcgcctctgcgtcagatcgccttttacggcaagggcggtatcggcaagtccaccacctctcagaacacgctggccgcgctggtcgagctggatcagaggatcctgatcgtcggctgcgacccgaaggccgactcgacccgcctgatcctgcacgcaaaggcccaggacaccgtcctgcatctggccgcc gaggccggctcggtcgaggatctggagctcgaggacgttctcaagatcggctacaagaacatcaagtgcgtcgagtccggcggtccggagccggggggtcggctgcgccggccgcggcgtcatcacctcgatcaacttcctggaagagaacggcgcctacgacgacgtggactatgtgtcctacgacgtgctgggcgac gtggtctgcggcggcttcgccatgccgatccgcgagaacaaggcccaggaaatctacatcgtcatgtccggcgagatgatggcgctgtacgccgccaacaacatcgccaagggcatcctgaagtacgcgcacagcggcggcgtccgtctcggcggcctgatctgcaacgagcgccagaccgacaaggaatgggat ctggccgacgcgctggccaagcgcctgggctccaagctgatccacttcgtgccgcgcgacaacatcgtccagcacgccgagctgcgccgcatgacggtcatcgagtacgccccggacagcaagcaggccggcgaataccgcgcgctcgccaacaagatccatgcgaactccggccagggttgcatcccgaccccgatcaccatgga agagctggaagagatgctgatggacttcggcatcatgaagaccgaggagcagcagctcgccgagctcgccgccaaggaagcggcgaaggccggcgcctga 169 atgagcctgtccgagaacaccacggtcgacgtcaagaacctcgtcaacgaagtcctcgaagcctatcccgaaaaatcccgcaagcgccgcgccaagcacctgaacgtgctggaggccgaggccaaggactgcggcgtcaagtcgaacgtcaagtccatccccggcgtcatgaccatccgcggctgcgcctatgcc ggctccaagggcgtggtgtggggtccgatcaaggacatgatccacatctcccacggtccggtcggctgcggctactactcctggtccggccgccgcaactactacatcggcgacaccggtgtggacagctggggcacgatgcacttcacctccgacttccaggagaaggacatcgtcttcggcggcgacaagaagctgcacaaggtcatcgaggaa atcaacgagctgttcccgctggtgaacggcatctcgatccagtcggaatgcccgatcggcctgatcggcgacgacatcgaggctgtcgcccgcgccaagtcggcggaaatcggcaagccggtcatccccgtgcgctgcgaaggcttccgcggcgtgtcccagtcgctgggccaccacatcgccaacgacgccat ccgagactgggtgttcgagaagacggaacccaaggccggcttcgtctccaccccctatgacgtcaccatcatcggcgactacaacatcggcggcgacgcctggtcgtcccgcatcctgctggagaggagatcggcctgcgcgtgatcgcccagtggtcgggcgacggcacgctcgccgaactggagaacacgccgaagg ccaaggtcaacctgatccactgctaccgctcgatgaactacatcgcgcgccacatggaagaagaagttcaacattccttggatggaatacaacttcggcccgagccagatcgccgaatccctgcgcaagatcgccgctctcttcgacgacaagatcaaggagaacgccgagaaggtcatcgccgctaccagccgatggtcgatgc ggtcatcgccaagtacaagccgcggctcgaaggcaagaaggtcatgatctacgtcggcggcctgcgtccccgccacgtcgtcgatgcctaccatgacctcggcatggagatcaccggcaccggctacgagttcgcccacaacgacgactatcagcgcacgcagcactacgtgaaggaaggcacgctgatctacgacgt caccgcgttcgaactggagaagttcgtcgaggcgatgcgtcccgacctcgtcgcgtcgggcatcaaggaaaagtacgtgttccagaagatgggcctgccgttccgccagatgcacagctgggactactccggcccgtaccacggctatgacggcttcgcgatcttcgcccgcgacatggacctggccatcaacaaccc cgtctggggcgtgatgaaggccccgttctga 170 atgctccaggacaagatccaggatgtcttcaacgaaccgggctgcgcgaccaaccaagccaaatcggccaaggagaagaagaagggctgcaccaagtcgctgaaaccgggggcggcagccggcggctgcgcctatgacggggcgatgatcgtgctccagccgatcgccgacgccgcccatctggtccatggccccatcgcctgcct cggaaacagttgggacaaccgcggctccaaatcctccggctcgcagctctaccgcaccggcttcaccaccgatctgtcggaactggacgtcatcggcggcggcgagaagaagctctaccgcgccatcaaggagatcgttcagcaatacgacccgccggccgtcttcgtctatcagacctgcgtgcccgccatgaccggcg acgacatcgccgcggtctgcaagttcgccacgcagaagctgggcaagccggtgatcccggtggactcgccgggcttcgtcgggtcgaagaatctcggcaacaagctggccggcgaagccctgctggagcatgtcatcggcacggtcgaaccggactacaccaccccgaccgacgtctgcatcatcggcgaatacaaccttgccggcgag ctgtggctggtcaagccgctgctggacgagatcggcatccgcctcctgtcctgcatttccggcgacggccgctaccgggaggtggcgcaggcccaccgcgcccgcgtcaccatgatggtgtgcagccaggcgctggtgaatgtcgggcgcaagatggaggagcgctacggcatcccctatttcgaggggtccttctacgg cgtgtccgacatgtcggacaccctgcgcaccatgacccgcatgctggtggagcgcggcgccgacaagggcctgatcgaccgggcggagggcgtgatcgcgcgggaggaaagccgggtctggcgccggctggaaccctacaagccgcgcttcgacggcaagcgcgtccttctcttcaccggcggcgtcaaga gctggtcgatggtcagcgcgctggagggtgcggggctgaccatcctcggcacctccaccaagaaatcgaccagggaggacaaggagcgcatcaagaagatgaagggcgaagagttccaccagtgggacgatttgaagccgcgcgacatctacaggatgctggccgacgatcaggccgacatcatgatgtccggcggccgctcgcagttcatctc gctgaaggccaaggttccctggctcgacatcaaccaggagcgccaccacgcctatgccggctatgacggcatcgtcaatctctgcgaggagatcgacaaaacgctgtcgaatccgatctggcgtcaggtgcgtcagccggcaccgtgggagtccggcgcgtcctccacccttctggcttcctcgatggcggcggagtga 171 atgtcccacatccagcgcttcccctccgccgccaaggccgcctccaccaacccgctgaagatgagccagccgctgggtgcggctctggcctatctcggcgtcgaccgctgcctgccgctgttccatggctcgcaggctgcaccgccttcggggctggtcctgctggtgcgccatttccgcgaggcgatcccgctccaga ccacggcgatggatcaggtcgccaccatcctcggcggctacgacaatctggagcaggcgatccgcaccatcgtcgagcgcaaccagcccgccatgatcggcgtcgccaccaccggcgtcaccgagaccaagggcgaggatatggccggacagtacacgctgttccgccagcgcaacccgacttggccgacacggccctggtcttc gccaacacccccgacttcgccggcggcttcgaggacggcttcgccgccgcggtcaccgcgatggtcgagcggttggtcgaaccgtcgccggtgcgcatcccgacccaggtcaacgtgctggccggctgccatctgtcccccggcgacgtggaggaactgcgcgacatcatcgaaggcttcggcctgtcgccga tcttcctgcccgacctgtcgctgtcgatggcgggccgccagccggccgacttcaccgccacctcgctgggcggcgtgaccgtcgatcagatccgcgccatgggcgcttcggccctcaccatcgtggtcggtgagcatatgcgggtggccggtaacgcgctggagctgaagaccgacgtgcccagccattt cttcaaccgcctgaccgggctggaggcgacggacaagctggtccggctgctgatggagttgtcgggcaagccggcgcccgcccggctgcggcgccagcgcgaaagcctggtcgatgccatgctcgacgggcatttcttctacagccgcaagcgcgcatcgccgtcgcgctggagcccgacctgctctatgccgt caccggcttcctcgccgacatgggggccgaggtgatcgccgcggtgtccccgacgcagagcccggtgctggagcggttgaaggccgccaccatcatggtcggcgatcattccgacgtggagacgctggcccgcgacgccgacctgatcgtctccaactcgcacgggcggcagggagccgcgcggatcggcgtggct ctgcaccgcatgggcctgccgctgttcgaccggctgggggccggcctgcgcgtccaggtcggctaccgcggcacgcgggaactgctgtgcgacatcggcaacctgttcctcgcccgcgagatggaccacgagcacggccacgagccacgaccacggggaatcccacggctgcggaggcggatcatgcggatgcaac gccgtctga 172 atgaccgacaagctttcgcagagcgccgacaaggtcctcgaccactacaccctcttccggcagcccgaatacgcggcgatgttcgagaagaagaagaccgagttcgagtacggccattcggacgaggaagtcgcccgcgtgtccgaatggaccaagtccgaggactaaggcgaagaacttcgcccgtgaagcggtcgtcat caacccgaccaaggcctgccagccgatcggcgcaatgttcgccgcccagggcttcgaaggcaccctgcccttcgtccacggctcccagggctgcgtcgcctattaccgcacccacctgacccgtcacttcaaggaccgaacagcgcggtctcctcgtcgatgacggaggacgcggcggtgttcggcggcctga acaacatgatcgacggcctggcgaacgcctatgcgctctacaagccgaagatgatcgcggtgatgaccacctgcatggccgaagtcatcggcgacgatttgcagggcttcatcgccaatgcgaagaccaaggacagcgtcccggccgacttcccggtcccctacgcccacaccccggccttcgtcggcagccacatcgtcggct acgacaacatgatcaaggggatcctgaccaacttctggggtacgtcggagaatttcgacacacccaagaccgagcagatcaacctgatcccgggcttcgacggcttcgccgtcggcaacaaccgcgaactgaagcgcatcgccggcgaattcggcgtgaagctgcaaatcctgtccgacgtgtccgacaatttcgacacgcc gatggatggcgagtaccgcatgtatgacggcggcaccaccatcgaggagaccaaggaggccctgcacgccaaggccaccatctccatgcaggagtacaacacgacccagaccctgcaattctgcaaggagaagggtcaggaagtcgccaagttcaactacccgatgggcgtcaccggcaccgacgagctgctgctgaagctcgccgaactgtcgggca agccggtcccggccagcctgaagctggagcgcggccgtctggtcgacgccatcgccgacagccacacacccacatgcacggcaagcgcttcgccgtctatggcgacccggacttctgcctgggcatgtccaagttcctgctggagctgggtgcggagccggtgcacatcctgtcgacgtcgggctccaagaagtgggagaagcagg tccagaaggtgctggacggctcgcccttcggcgcctcgggcaaggcccatggcggcaaggatctgtggcacctgcgttcgctgatcttcaccgacaaggtggactacatcatcggcaacagctacggcaagtatctggagcgcgacaccaaggttccgctgatccgcctgacctacccgatcttcgaccgccaccaccaccgctaccc gacctggggctaccagggcgcgctgaacgtgctggtacggatcctggaccggatcttcgaggacatcgacgccaacaccaacatcgtcggccagaccgactactcgttcgacctgatccgctga 173 atgttgacctctgatattgttggcaaattgcgctgcatcgcagcagaccccaaagcgggcatcgcaaggggcctcgacaccgggacgacgaagatcggtcccgtttgggagggtgacgtgggcgacaccgtggatttcgaagcgctgcgccagcgggcggtccactccctgttcgaacatctggaatccatgtgc gtcggcgccgtcgccgtcgaccacaccggccgcatcgcctggatggacgagaagtacaaggctgctgggcgttcccgacgacccgcgcggccggcaggtggaggacgtcatccccaacagccagctgcgccgggtgatcgacagcggccagccgcagccgctggacatcatggagttcgacgaccggtccttcgtggt gacgcggatgccgctgttcggcaccgacggttcgatcatcggcgccatcggcttcgtgctgttcgaccgcgccgaatatctccgcccgctggtccgcaaatacgagaagatgcaggaggagctggcccgcacccagcaggagctggcgcatgagcgccgcgccaaatactccttctcgcagttcctgggcgccagcga atcgatccgcgagatcaagcggctggggcgccgcgccgcccagatggattcgaccgtcctgctgctgggcgaaaccgggaccggcaaggagctgctggcccaggccatccattccgccagcccgcgggcgtccaagcccttcgtcggcgtcaatgtcgccgccattccggaaaccctgctggaggcggagttctt cggcgtcgcccccggcgccttcaccggcgccgaccgccgccaccgcgacggcaagttccagctcgccaacggcggcaccctgttcctcgacgagatcggcgacatgccgctgccggtgcaggccaagcttctgcgcgtgctgcaggagcgggagatcgagccgctcggctccaacaaggtggtgcgggtcgatg tccgcatcatcgccgccaccagccgtgacctgcacgccctggtgcgtgagaagcagttccgcgccgacctctattaccggctgaatgtggtgccgatcaccctgccgccgctgcgcgaccggccggaggacatcgagcatcgccgaccgcatcctggaacagctggcgatccagcagggcacgccgccgcgcgagctgct ggaatcggcggtgcaggtgctgcgcgactatgactggcccggcaatgtgcgcgagctttacaacaccgctggaacgggtggtggcgctgaccgatgcgccgatcctgaccgcgccgcacatccgcagcgtgctgcccggccagcatccggccggcgcgtcggccctgccgctggcggccggcgcgcggcc gttgcaggaggtgctgcacgccgccgagcgccacgccatcgccgcggcgcttgaggaggcgaacggcgtcaaggcgcgggcggcgaagctgctgggcatttcgcgcgcgtcgctgtacgaacgcatggtgacgctggggttgggggcgacgcagtag 174 atgccgagtcccatcgcgttctcaagccccttgccgaagcctttcgacagcgcgcaggcggcgctggggatggagcgctggcgccagcaggccgccgcggcggagccggagaacccgcgcctgggcggaagccttcgccgattcggagaccggccgggcgctgatcggggcggtgtgcggcaacagcccgtatctc ggccacagcctgacgcgggagttgcccttcgtcgcccgtacagtgcaggacggcttcgacgacaccttcgccgcgctgatcgccgctctctccatgccgagcatggcgaggagaaatcgatggaccggctgatggccggcctgcgggtggcgaagcggcgggcggcgctgctgatcgcgctggccgacatcgcc ggcgcgtggccgctgttccgcgtcaccggcgccctgtcggagctggcggagacgggggtgcagctggccgcgaatttcctgctgcgccgcgccagggaggcggggacgctgacgctgccggatccgcagcgaccgtgggtcggttcgggcctgatcgtttgggcatgggtaagcttggcgggcgcgaact caactattccagcgacatcgacctgatcgtcctgtatgacgacgctgttgtgcagacgccccagccggacaacctcgcgcgaaccttcatcaggctcgcacgcgatcttgtccgcattatggatgaacggaccaaggacggctacgtcttccgcaccgaccttcggcttaggcccgatcccggcgccacgccgctggc ggtttccgtctccgcagccgaaatttattacggcagcgtcggtcagaactgggaacgcgcggcgatgatcaaggcccgtcccatcgccggcgatctggaggcgggcgcctcctttgtccgcttcctggagcccttcgtctggcgccgcaacctggatttcgccgccatccaggacatccattcgatcaaacgccagat caacgcccacaagggccaccgcgaggtgacggtcaacggccacgacatcaaggtcggccgcggcggcatccgcgagatcgagttcttcgcccagacccagcagctgatcttcggcgggcgcgacccgcgcgtgcgaatcgctccgaccctgatggcgaacgaggcgctgcgcgacgtcggccgcgtgccg ccgcagacggtggaagagcttgccggggcctatcatttcctgcgccgtgtcgaacatcgcatccagatgatcgacgaccagcagacccatcgtattcccgccgacgatgccggggtggcgcatttggccaccttcctcggctatgacgaccccgccgccttccgggcggaactgctggcgacgctggggcaggtggaggaccgctat gccgagctgttcgaggaggcgccgtcgctttccggccccggcaatctggtcttcaccggcaccgaccccgatccgggcacgatggagacgctgaagggcatgggcttcgccgatccggcccgcgtcatcagcgtggtgtcggcctggcatcgcggccgctaccgcgccacccggtcgggccggggcgcgggagctg ctgacggagctgacgccggccctgctgagtgcgctgaccaagaccccggccccgattcggcgctgatgaacttcgacgatttcctcggcaagctgccggccggcgtcggtctgttctcgctgttcgtcgccaatccctggctcctggagctggtggcggagatcatgggcatcgcgccgcagatggcgcagacgctgtc gcgcaacccgtcgctgctcgacgccgtgctgtcgccggacttcttcgacccgctgccgggcaaggaggacgggctggccgacgaccacgcccgcgtgatggcgccggcccgcgatttcgaggatgcgctgaccctgtcgcggcgctggaccaacgaccagcgcttccgcgccggggtgcatatcctg cgcggcatcaccgatggcgaccgctgcggcgccttcctggccgatctggccgacatcgtcgtccccgaccttgcccgccgggtggaggaggagttcgcccagcgccacggccatattcccggcggcgcctgggtggtggtggcgatgggcaagctcggcagccggcagctgaccatcacgtccgacatcgacctgatcg tcatctacgatgtggcgccgggccaagggggcgggggcggtccccgcttgtcggatggtgccaagccgctgtcgcccaacgagtattacatcaagctgactcagcgtctgaccaacgccattaccgcgccgatgggcgacggccggctctacgaggtcgacatgcggctgcgcccgtcgggcaacgccgggccgctcgccacc tcgctggacgctttcctgaaatatcaggcgaccgatgcctggacctgggagcatatggccctgacccgcgcccgggtgatcggcggtgatgcggagctggccgggcgggtgtcggcagcgatccgctcggtgctgacggcgccgcgcgatgccgaccggctgctgtgggacgtggccgacatgcggcggc ggatcgagaaggagttcgggacgaccaatgtctggaacgtcaaatacgcccgcggcggcctgatcgacatcgagttcatcgcccagtacctgcaactgcgccatggtcacgagcggccggacatcctgcacatcggcaccgccaaggcgctgggctgcgccgcccggacgggcgcgctggcgccggaggtggcggag gatctggagacgacgctgcggctgtggcggcgggtgcagggctttctgcggttgaccaccgccggggtgctcgatcccaatcaggtgtcgcccagcctgctggccggggctggtccgcgccgcctttcctgctgactttcagggcgagcgtgagcctggcactgttgacttccccgaactggaccacaaaatccgtg ccgtcgccgcccgcgcccatggtcatttcaagaccctggtcgaggaaccggcgggccgtctggccccacccgccaccacgcctccagcctga 175 atgaaccgtctgttccttatggccgcaccgatgatggcggttgctctgggcgcggtcggcatgccggccgcagcccttgcccaggatccggcggctgccgccgctgccgcggctgcggctgcggctgccgccgctgctgccgcaccggcggctccggcgctgaatggcggcgacaccgcctggatgctcatctccaccgc gctggtgctgatgatgaccatccccggcctggcgctgttctacggcggcatggtccgcaagatgaacgtgctgtcgacggtgatgcagagcttcgccatcacctgcctgatcagcgtcctgtggtacgtcatcggctacagcctggccttcaccggcaccggtgcctatgtcggcggtctcgaccggctgttcct caacgggctcgacttcacgaaggccttcgtgctgggcgaggcgaccgggtcgggcgtcccgacgaccatccccgagccggtctcatgatgttccagatgacctttgcgatcatcaccccggccctgatcaccggcgccttcgccgaccgcatgaagttctcctccctgctggtcttcaccgcgctgtggtcgatcgt ggtctatgcgccgatcgcccactgggtctggtacccgtcgggcttcctgttcggcctgggcgtgctggacttcgccggcggcacggtcgtgcacatcaacgccggcgtcgccggcctggtcgccgcgctggtgatcggcaagcgcaagggctacccgaaggaagccttcatgccgcacaacctggtgctgt cgctgatcggcgcctcgctgctgtgggtcggctggttcggcttcaacgccggttcggccctgaccgccggtccgcgtgccggcatggcgctggccgccacgcacatcgccaccgccggtgccgccatgggctggctgttcgcggagtggatcgtcaagggcaagccgtcgatcctcggcatcatctccggcgccgt cgccggcctggtcgcggtgaccccggccgccggcttcgtcgacccgacgggcgccatcgtcatcggcatcgtcgccggcgtggtctgcttctggtcggccaccagcctcaagcacatgctgggctatgacgacagcctggacgccttcggcgtgcacggcgtcggcggcctgatcggcgccatcctgacc ggcgtcttcgccaagatgtcggtgtccaacagcgaaggcggcttcgcctccgtcctgcaggccgacccgaaggccacgctgggcctgctggaaggcaacgccgccgccgtctggatccaggtccagggcgtcctctacaccatggtctggtgcgccatcgccaccttcgtcctgctgaagaatcgtcgatgt ggtcatgggcctgcgcgtcgaagaggatgtggagcgcgacggtctcgacctcgccctgcatggcgagagcatccactaa 176 atggatgcggcaaagacgggtggcgacgtccttttcgtgctgatgggcgcggtgatggtgctggcgatgcattgcggcttcgccctgctggaggtcgggacggtccggcgcaagaatcaggtcaacgcgctggtgaagatcctgtcggacttcgccatgtcgaccatcgcctatttttttcgtcggtt atgccgtggcctacggcatcgacttcttcgccgacgcccacacgctggtcggcaagggaagcggcgggttcgcggcctatggctacgatctggtgaagttcttcttcctggcgaccttcgccgccgcggtgccggccatcgtctcgggcggcatcgccgagcgtgctaggttctggccgcaggccgccgccacg ctggcgctgatcgcgctgttctatccattgctggaaggcacggtctggggcacccgcttcggcctgcaaagctggatggccgcgaccttcggccagccttcccgacttcgccggatctgtggtggtgcatgccttcggcggctgggtggcgctgggtgccgtgctgaacctcggcaaccgccgcggccgctacc gtccgaacggctcgctgatcgccattccgccgtcgaacatccccttcctggcgctgggcgcctgggtgctgtgcgtggggtggttcggcttcaacgtgatgagcgcccaggtgctggatggcgtgacgggtctggtggcgctgaactcgctgatggcgatggtcggcggcatcgtcacctcgctggt gatcagccgcaccgatcccggcttcgtccacaacggcgcgctggccggtctggtggcggtctgcgccgggtccgacgtgatgcacccgctgggcgcgctggtcaccggcggcatcccgggctgctgttcgtctgggccttcaacaaatgccagatcgactggaagaatcgacgacgtgctgggcgtct ggccgctgcacggtctgtgcggcctgaccggcggcctgctggccggcgtcttcgggcaggaggcactgggcggccttggcggcgtgtcgatcctcagccagatcgtcggcacggcaagcggcgccagcttcggattcgtctcgggtctggcggtctacggcctgctgcgcgtcaccgtcggcatcc gcctcgatcccgagcaggagtacaagggcgccgacttgtcgttgcaccatatcaccgcgtacccggaagaggacgcgccgaccctgtaa 177 atgaccctgaatatgatgatggattcttggttctctggagcgctttatcggcatcctgactgaagaatttgcaggcttcttcccaacctggcttgcacccgtgcaggtagttgtgaatcactgattcgcaggctgaatacgttaacgaattgacccgtaaactgcaaaatgcgggcattcgtgtaaaagcagacttgagaa acgagaagattggctttaaaatccgcgagcacactttacgtcgtgtcccttatatgctggtttgtggtgacaaagaggtcgaagccggcaaagttgctgtgcgtacccgtcgcggtaaagacctgggtagcctggacgtaaatgatgttatcgagaagctgcaacaagagattcgcagccgcagtcttcaacaactgg aggaataaggtattaaaggcggaaaacgagttcaaacggcgcgtcccaatcgtattaatggcgagattcgcgccacggaagttcgcttaacaggtctggaaggcgagcagcttggtattgcgatagaactcacttcacgccccgaagggggaagctgcctgaccctacgattcccgctatttcattcactgaccggaggttcaaa atga 178 accggataagagagaaaagtgtcgacgtcggtccggttgatattgaccggcgcatccgccagctcgcccagtttttggtggatctgtttggcgattttgcgggtcttgccggtgtcggtgccgaaaaaaataccaatatttgccataacacacgctcctgttgaaaaagagatcccgcggggaaaatgcggtgaac atgtcagctattgcgaagagtgtgccagttttgctcacgggcaaaagctgcaccagaatgggtattaatgcaccagcctggcgctttttttcgcggcacgtcccctcgctaatgcccgtctggcgcggctttgacgctgataaggcgctgaataccgatctggatcaaggttttgtcgggttatcgtccaaaagg tgcactctttgcatggttataagtgcctgacatggtgtccgggcgaacgtcgccaggtggcacaaattgtcagaactacgacacgactaaccgaccgcaggagtgtgcgatgaccctgaatatgatgatggattcttggttctctggagcgctttatcggcatcctgactgaagaatttgcaggcttcttcccaacctggcttgcaccc gtgcaggtagttgtgatgaacatcactgattcgcaggctgaatacgttaacgaattgacccgtaaactgcaaaatgcgggcattcgtgtaaaagcagacttgagaaacgagaagattggctttaaaatccgcgcacactttacgtcgtgtcccttatgctggttgtggtgacaaagaggtcgaagccggcaaag ttgctgtgcgtacccgtcgcggtaaagacctgggtagcctggacgtaaatgatgttatcgagaagctgcaacaagagattcgcagccgcagtcttcaacaactggaggaataaggtattaaaggcggaaaacgagttcaaacggcgcgtcccaatcgtattaatggcgagattcgcgccacggaagttcgcttaaca ggtctggaaggcgagcagcttggtattgcgatagaactcacttcacgccccgaagggggaagctgcctgaccctacgattcccgctatttcattcactgaccggaggttcaaaatgacccagcgaaccgagtcgggtaataccgtctggcgcttcgatttatcccagcagttcaccgcgatgcagcggataagcgtggttctca gccgggcgaccgaggttgaacagacactccagcaggtgctgtgcgtattgcacaatgacgcctttttgcagcacggcatgatctgtctgtacgacagccagcaggcgattttgactattgaagcgttgcaggaagccgatcagcagttgatccccggcagctcgcaaattcgctaccgtccgggtgaagggctggtcggg acggtgctttcgcaggggcaatcgttagtgctggcgcgtgtggctgacgatcagcgctttcttgaccgcctgggactgtatgattacaacctgccgtttatcgccgtgccgctgatagggccggatgcgcagacttttggcgtgctgacggcgcaaccgatggcgcgttacgaagagcggttacccgcctgc acccgctttctggaaacggtc 179 tccctgtgcgccgcgtcgccgatggtggccagccaactggcgcgctacccgatcctgctcgatgaactgctcgacccgaacacgctctatcaaccgacggcgatgaacgcctatcgcgatgaactgcgacaatacctgttgcgcgtgccggaagaggatgaagagcagcaactggaggcgctacggcagtttaag caggcgcagttgttgcgcgtagcggcggcggatatcgccggtacgttacccgtcatgaaagtgagcgatcacttaacctggctggcggaagcgattatcgatgcggtggtgcagcaagcctggaaccagatggtggcgcgttacggccagccgacgcatctgcacgatcgcgaagggcgcggtttcgccgt ggtcggttacggcaaacttggcggctgggaattaggttacagctccgatctggatctggtgttcctgcacgactgccccatggatgtgatgaccgatggcgagcgtgaaatcgatggccgccagttctatttgcgcctcgcgcagcgcgtgatgcacctgttcagcacgcgcacgtcgtccggcattcttttga agtcgatgcgcgtttgcgcccgtccggcgcggccggaatgctggtgaccactgcggaagcgttcgccgattatcaaaaaaatgaagcctggacatgggcatcaggcgctggcgcgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatatcctga tgacctcccgcgatgccgctaccctgcaaaccgaagtgcgggaaatgcgtgagaaaatgcgcgcccatcttggtaacaagcacaaagaccgttcgatctgaaagccgatgaaggcggtatcaccgatattgagttttcgctcagtatctggtgctgcgctttgcccatgagaagccgaaactgacgcgctgg tcggataatgtgcgcatcctcgaagggctggcgcaaaacggcatcatggatgagcaggaagcgcaggcattgacgctggcgtacaccacgttgcgtgatgagctgcaccacctggcgctgcaagagctgccaggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttatcaaaaccagctgggacaagt ggctggtggaaccgtgcgccccggcgtaa 180 taaagcgagcgctcacttacgtgatctgttgacgcagtccgaagcgaccattacttcagccgtttcagcagatacggcggtgtggagtgcgcaatcagccctggcgaaactggtgctcaccgagtggttagtgacgcagggctggcgaaccttccttgatgaaaaagcgcaggctaagtttgccgactccttta aacgctttgctgacgttcatctgtcacgcagcgccgccgagctgaaaaaagccttgcccagccgctgggcgacagctatcgcgaccagttaccgcggctggcgcgtgatatcgacagcgcgttattgctggccggacattacgatcgcgcgcgcgccgtggagtggctggaaaactggcaggggct tcagcacgctattgaaacgcgccagagagttgaaatcgaacatttccgtaataccgccattacccaggagccgttctggttgcacagcggaaaacgttaacgaaaggatatttcgcatgtccctgtgcgccgcgtcgccgatggtggccagccaactggcgcgctacccgatcctgctcgatgaactgctcgacccgaac acgctctatcaaccgacggcgatgaacgcctatcgcgatgaactgcgacaatacctgttgcgcgtgccggaagaggatgaagagcagcaactggaggcgctacggcagtttaagcaggcgcagttgttgcgcgtagcggcggcggatatcgccggtacgttacccgtcatgaaagtgagcgatcacttaacctggctggc ggaagcgattatcgatgcggtggtgcagcaagcctggaaccagatggtggcgcgttacggccagccgacgcatctgcacgatcgcgaagggcgcggtttcgccgtggtcggttacggcaaacttggcggctgggaattaggttacagctccgatctggatctggtgttcctgcacgactgccccatggatgtgatgaccga tggcgagcgtgaaatcgatggccgccagttctatttgcgcctcgcgcagcgcgtgatgcacctgttcagcacgcgcacgtcgtccggcattctttatgaagtcgatgcgcgtttgcgcccgtccggcgcggccggaatgctggtgaccactgcggaagcgttcgccgattatcaaaa aaatgaagcctggacatgggagcatcaggcgctggcgcgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatatcctgatgacctcccgcgatgccgctaccctgcaaaccgaagtgcgggaaatgcgtgagaaaatgcgcgcccatcttggtaacaag cacaaagaccgtttcgatctgaaagccgatgaaggcggtatcaccgatattgagtttatcgctcagtatctggtgctgcgctttgcccatgagaagccgaaactgacgcgctggtcggataatgtgcgcatcctcgaagggctggcgcaaaacggcatcatggatgagcaggaagcgcaggcattgacgctggcgtacaccacg ttgcgtgatgagctgcaccacctggcgctgcaagagctgccaggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttatcaaaaccagctgggacaagtggctggtggaaccgtgcgccccggcgtaagtgtggtatcatcgcgcgcaaattttgtatctctcaggagacaggaatgaaagtt acgctgccagagttcaatcaagccggtgtcatggtggtgggtgatgtgatgctggatcgctactggtacggcccaaccagccgcatttctccggaagcgccagttccggttgttaaagtcgatactatttgaagagcgaccgggcggtgcggcaaacgtggcgatgaacattgcctcgctgggcgcaacggcgcgtct ggttggcctgactggcattgatgatgcggcgcgcgcgctgagcaaagcgctggcggatgttaatgttaaatgtgacttcgtttctgttccgactcaccccaccatcactaagctgcgcgtgctgtcgcgtaaccagcaactgattcgc 181 atgaccctgaatatgatgatggatgccagccgttctgtaataataaccggacaattcggactgattaaaaaagcgccctcgcggcgcttttttttattctcgactccatttaaaataaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgtttaacacgcgttttttaacctttta ttgaaagtcggtgcttctttgagcgaacgatcaaatttaagtggattcccatcaaaaaaatattctcaacctaaaaaagtttgtgtaatacttgtaacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgcaactcacttcacgccccgaagggggaagctgcctgaccctacgattc ccgctatttcattcactgaccgggaggttcaaaatga 182 accggataagagagaaaagtgtcgacgtcggtccggttgatattgaccggcgcatccgccagctcgcccagtttttggtggatctgtttggcgattttgcgggtcttgccggtgtcggtgccgaaaaaaataccaatatttgccataacacacgctcctgttgaaaaagagatcccgcggggaaaatgcggtgaac atgtcagctattgcgaagagtgtgccagttttgctcacgggcaaaagctgcaccagaatgggtattaatgcaccagcctggcgctttttttcgcggcacgtcccctcgctaatgcccgtctggcgcggctttgacgctgataaggcgctgaataccgatctggatcaaggttttgtcgggttatcgtccaaaagg tgcactctttgcatggttataagtgcctgacatggtgtccgggcgaacgtcgccaggtggcacaaattgtcagaactacgacacgactaaccgaccgcaggagtgtgcgatgaccctgaatatgatgatggatgccagccgttctgtaataataaccggacaattcggactgattaaaaaagcgccctcgcggcgctttttttata ttctcgactccatttaaaataaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgttttaacacgcgttttttaaccttttattgaaagtcggtgcttctttgagcgaacgatcaaatttaagtggattcccatcaaaaaatttctcaacctaaaaaagtttgtgtaat acttgtaacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgcaactcacttcacgccccgaagggggaagctgcctgaccctacgattcccgctatttcattcactgaccggaggttcaaaatgacccagcgaaccgagtcgggtaataccgtctggcgcttcgatttatcccagcagttcacc gcgatgcagcggataagcgtggttctcagccgggcgaccgaggttgaacagacactccagcaggtgctgtgcgtattgcacaatgacgcctttttgcagcacggcatgatctgtctgtacgacagccagcaggcgattttgactattgaagcgttgcaggaagccgatcagcagttgatccccggcagctcgcaaattcg ctaccgtccgggtgaagggctggtcgggacggtgctttcgcaggggcaatcgttagtgctggcgcgtgtggctgacgatcagcgctttcttgaccgcctgggactgtatgattacaacctgccgtttatcgccgtgccgctgatagggccggatgcgcagacttttggcgtgctgacggcgcaaccgatggc gcgttacgaagagcggttacccgcctgcacccgctttctggaaacggtc 183 tccctgtgcgccgcgtcgccgatggtggccagccaactggcgcgctacccgatcctgctcgatgaactgctcgacccgaacacgctctatcaaccgacggcgatgaacgcctatcgcgatgaactgcgacaatacctgttgcgcgtgccggaagaggatgaagagcagcaactggaggcgctacggcagtttaag caggcgcagttgttgcgcgtagcggcggcggatatcgccggtacgttacccgtcatgaaagtgagcgatcacttaacctggctggcggaagcgattatcgatgcggtggtgcagcaagcctggaaccagatggtggcgcgttacggccagccgacgcatctgcacgatcgcgaagggcgcggtttcgccgt ggtcggttacggcaaacttggcggctgggaattaggttacagctccgatctggatctggtgttcctgcacgactgccccatggatgtgatgaccgatggcgagcgtgaaatcgatggccgccagttctatttgcgcctcgcgcagcgcgtgatgcacctgttcagcacgcgcacgtcgtccggcattcttttga agtcgatgcgcgtttgcgcccgtccggcgcggccggaatgctggtgaccactgcggaagcgttcgccgattatcaaaaaaatgaagcctggacatgggcatcaggcgctggcgcgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatatcctga tgacctcccgcgatgccgctaccctgcaaaccgaagtgcgggaaatgcgtgagaaaatgcgcgcccatcttggtaacaagcacaaagaccgttcgatctgaaagccgatgaaggcggtatcaccgatattgagttttcgctcagtatctggtgctgcgctttgcccatgagaagccgaaactgacgcgctgg tcggataatgtgcgcatcctcgaagggctggcgcaaaacggcatcatggatgagcaggaagcgcaggcattgacgctggcgtacaccacgttgcgtgatgagctgcaccacctggcgctgcaagagctgccaggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttatcaaaaccagctgggacaagt ggctggtggaaccgtgcgccccggcgtaa 184 taaagcgagcgctcacttacgtgatctgttgacgcagtccgaagcgaccattacttcagccgtttcagcagatacggcggtgtggagtgcgcaatcagccctggcgaaactggtgctcaccgagtggttagtgacgcagggctggcgaaccttccttgatgaaaaagcgcaggctaagtttgccgactccttta aacgctttgctgacgttcatctgtcacgcagcgccgccgagctgaaaaaagccttgcccagccgctgggcgacagctatcgcgaccagttaccgcggctggcgcgtgatatcgacagcgcgttattgctggccggacattacgatcgcgcgcgcgccgtggagtggctggaaaactggcaggggct tcagcacgctattgaaacgcgccagagagttgaaatcgaacatttccgtaataccgccattacccaggagccgttctggttgcacagcggaaaacgttaacgaaaggatatttcgcatgtccctgtgcgccgcgtcgccgatggtggccagccaactggcgcgctacccgatcctgctcgatgaactgctcgacccgaac acgctctatcaaccgacggcgatgaacgcctatcgcgatgaactgcgacaatacctgttgcgcgtgccggaagaggatgaagagcagcaactggaggcgctacggcagtttaagcaggcgcagttgttgcgcgtagcggcggcggatatcgccggtacgttacccgtcatgaaagtgagcgatcacttaacctggctggc ggaagcgattatcgatgcggtggtgcagcaagcctggaaccagatggtggcgcgttacggccagccgacgcatctgcacgatcgcgaagggcgcggtttcgccgtggtcggttacggcaaacttggcggctgggaattaggttacagctccgatctggatctggtgttcctgcacgactgccccatggatgtgatgaccga tggcgagcgtgaaatcgatggccgccagttctatttgcgcctcgcgcagcgcgtgatgcacctgttcagcacgcgcacgtcgtccggcattctttatgaagtcgatgcgcgtttgcgcccgtccggcgcggccggaatgctggtgaccactgcggaagcgttcgccgattatcaaaa aaatgaagcctggacatgggagcatcaggcgctggcgcgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatatcctgatgacctcccgcgatgccgctaccctgcaaaccgaagtgcgggaaatgcgtgagaaaatgcgcgcccatcttggtaacaag cacaaagaccgtttcgatctgaaagccgatgaaggcggtatcaccgatattgagtttatcgctcagtatctggtgctgcgctttgcccatgagaagccgaaactgacgcgctggtcggataatgtgcgcatcctcgaagggctggcgcaaaacggcatcatggatgagcaggaagcgcaggcattgacgctggcgtacaccacg ttgcgtgatgagctgcaccacctggcgctgcaagagctgccaggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttatcaaaaccagctgggacaagtggctggtggaaccgtgcgccccggcgtaagtgtggtatcatcgcgcgcaaattttgtatctctcaggagacaggaatgaaagtt acgctgccagagttcaatcaagccggtgtcatggtggtgggtgatgtgatgctggatcgctactggtacggcccaaccagccgcatttctccggaagcgccagttccggttgttaaagtcgatactatttgaagagcgaccgggcggtgcggcaaacgtggcgatgaacattgcctcgctgggcgcaacggcgcgtct ggttggcctgactggcattgatgatgcggcgcgcgcgctgagcaaagcgctggcggatgttaatgttaaatgtgacttcgtttctgttccgactcaccccaccatcactaagctgcgcgtgctgtcgcgtaaccagcaactgattcgc 185 atgaccctgaatatgatgatggatgccagccgttctgtaataataaccggacaattcggactgattaaaaaagcgccctcgcggcgcttttttttattctcgactccatttaaaataaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgtttaacacgcgttttttaacctttta ttgaaagtcggtgcttctttgagcgaacgatcaaatttaagtggattcccatcaaaaaaatattctcaacctaaaaaagtttgtgtaatacttgtaacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgcaactcacttcacgccccgaagggggaagctgcctgaccctacgattc ccgctatttcattcactgaccgggaggttcaaaatga 186 accggataagagagaaaagtgtcgacgtcggtccggttgatattgaccggcgcatccgccagctcgcccagtttttggtggatctgtttggcgattttgcgggtcttgccggtgtcggtgccgaaaaaaataccaatatttgccataacacacgctcctgttgaaaaagagatcccgcggggaaaatgcggtgaac atgtcagctattgcgaagagtgtgccagttttgctcacgggcaaaagctgcaccagaatgggtattaatgcaccagcctggcgctttttttcgcggcacgtcccctcgctaatgcccgtctggcgcggctttgacgctgataaggcgctgaataccgatctggatcaaggttttgtcgggttatcgtccaaaagg tgcactctttgcatggttataagtgcctgacatggtgtccgggcgaacgtcgccaggtggcacaaattgtcagaactacgacacgactaaccgaccgcaggagtgtgcgatgaccctgaatatgatgatggatgccagccgttctgtaataataaccggacaattcggactgattaaaaaagcgccctcgcggcgctttttttata ttctcgactccatttaaaataaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgttttaacacgcgttttttaaccttttattgaaagtcggtgcttctttgagcgaacgatcaaatttaagtggattcccatcaaaaaatttctcaacctaaaaaagtttgtgtaat acttgtaacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgcaactcacttcacgccccgaagggggaagctgcctgaccctacgattcccgctatttcattcactgaccggaggttcaaaatgacccagcgaaccgagtcgggtaataccgtctggcgcttcgatttatcccagcagttcacc gcgatgcagcggataagcgtggttctcagccgggcgaccgaggttgaacagacactccagcaggtgctgtgcgtattgcacaatgacgcctttttgcagcacggcatgatctgtctgtacgacagccagcaggcgattttgactattgaagcgttgcaggaagccgatcagcagttgatccccggcagctcgcaaattcg ctaccgtccgggtgaagggctggtcgggacggtgctttcgcaggggcaatcgttagtgctggcgcgtgtggctgacgatcagcgctttcttgaccgcctgggactgtatgattacaacctgccgtttatcgccgtgccgctgatagggccggatgcgcagacttttggcgtgctgacggcgcaaccgatggc gcgttacgaagagcggttacccgcctgcacccgctttctggaaacggtc 187 tccctgtgcgccgcgtcgccgatggtggccagccaactggcgcgctacccgatcctgctcgatgaactgctcgacccgaacacgctctatcaaccgacggcgatgaacgcctatcgcgatgaactgcgacaatacctgttgcgcgtgccggaagaggatgaagagcagcaactggaggcgctacggcagtttaag caggcgcagttgttgcgcgtagcggcggcggatatcgccggtacgttacccgtcatgaaagtgagcgatcacttaacctggctggcggaagcgattatcgatgcggtggtgcagcaagcctggaaccagatggtggcgcgttacggccagccgacgcatctgcacgatcgcgaagggcgcggtttcgccgt ggtcggttacggcaaacttggcggctgggaattaggttacagctccgatctggatctggtgttcctgcacgactgccccatggatgtgatgaccgatggcgagcgtgaaatcgatggccgccagttctatttgcgcctcgcgcagcgcgtgatgcacctgttcagcacgcgcacgtcgtccggcattcttttga agtcgatgcgcgtttgcgcccgtccggcgcggccggaatgctggtgaccactgcggaagcgttcgccgattatcaaaaaaatgaagcctggacatgggcatcaggcgctggcgcgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatatcctga tgacctcccgcgatgccgctaccctgcaaaccgaagtgcgggaaatgcgtgagaaaatgcgcgcccatcttggtaacaagcacaaagaccgttcgatctgaaagccgatgaaggcggtatcaccgatattgagttttcgctcagtatctggtgctgcgctttgcccatgagaagccgaaactgacgcgctgg tcggataatgtgcgcatcctcgaagggctggcgcaaaacggcatcatggatgagcaggaagcgcaggcattgacgctggcgtacaccacgttgcgtgatgagctgcaccacctggcgctgcaagagctgccaggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttatcaaaaccagctgggacaagt ggctggtggaaccgtgcgccccggcgtaa 188 taaagcgagcgctcacttacgtgatctgttgacgcagtccgaagcgaccattacttcagccgtttcagcagatacggcggtgtggagtgcgcaatcagccctggcgaaactggtgctcaccgagtggttagtgacgcagggctggcgaaccttccttgatgaaaaagcgcaggctaagtttgccgactccttta aacgctttgctgacgttcatctgtcacgcagcgccgccgagctgaaaaaagccttgcccagccgctgggcgacagctatcgcgaccagttaccgcggctggcgcgtgatatcgacagcgcgttattgctggccggacattacgatcgcgcgcgcgccgtggagtggctggaaaactggcaggggct tcagcacgctattgaaacgcgccagagagttgaaatcgaacatttccgtaataccgccattacccaggagccgttctggttgcacagcggaaaacgttaacgaaaggatatttcgcatgtccctgtgcgccgcgtcgccgatggtggccagccaactggcgcgctacccgatcctgctcgatgaactgctcgacccgaac acgctctatcaaccgacggcgatgaacgcctatcgcgatgaactgcgacaatacctgttgcgcgtgccggaagaggatgaagagcagcaactggaggcgctacggcagtttaagcaggcgcagttgttgcgcgtagcggcggcggatatcgccggtacgttacccgtcatgaaagtgagcgatcacttaacctggctggc ggaagcgattatcgatgcggtggtgcagcaagcctggaaccagatggtggcgcgttacggccagccgacgcatctgcacgatcgcgaagggcgcggtttcgccgtggtcggttacggcaaacttggcggctgggaattaggttacagctccgatctggatctggtgttcctgcacgactgccccatggatgtgatgaccga tggcgagcgtgaaatcgatggccgccagttctatttgcgcctcgcgcagcgcgtgatgcacctgttcagcacgcgcacgtcgtccggcattctttatgaagtcgatgcgcgtttgcgcccgtccggcgcggccggaatgctggtgaccactgcggaagcgttcgccgattatcaaaa aaatgaagcctggacatgggagcatcaggcgctggcgcgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatatcctgatgacctcccgcgatgccgctaccctgcaaaccgaagtgcgggaaatgcgtgagaaaatgcgcgcccatcttggtaacaag cacaaagaccgtttcgatctgaaagccgatgaaggcggtatcaccgatattgagtttatcgctcagtatctggtgctgcgctttgcccatgagaagccgaaactgacgcgctggtcggataatgtgcgcatcctcgaagggctggcgcaaaacggcatcatggatgagcaggaagcgcaggcattgacgctggcgtacaccacg ttgcgtgatgagctgcaccacctggcgctgcaagagctgccaggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttatcaaaaccagctgggacaagtggctggtggaaccgtgcgccccggcgtaagtgtggtatcatcgcgcgcaaattttgtatctctcaggagacaggaatgaaagtt acgctgccagagttcaatcaagccggtgtcatggtggtgggtgatgtgatgctggatcgctactggtacggcccaaccagccgcatttctccggaagcgccagttccggttgttaaagtcgatactatttgaagagcgaccgggcggtgcggcaaacgtggcgatgaacattgcctcgctgggcgcaacggcgcgtct ggttggcctgactggcattgatgatgcggcgcgcgcgctgagcaaagcgctggcggatgttaatgttaaatgtgacttcgtttctgttccgactcaccccaccatcactaagctgcgcgtgctgtcgcgtaaccagcaactgattcgc 189 atgaccctgaatatgatgatggatgccagccgcgtcaggttgaacgtaaaaaagtcggtctgcgcgcaaagcacgtcgtcgtccgcagttctccaaacgttaattggtttctgcttcggcagaacgattggcgaaaaaacccggtgcgaaccgggttttttttggataaagatcgtgttatccacagcaatcca ttgattatctcttctttttcagcatttccagaatcccctcaccacaaagcccgcaaaatctggtaaactatcatccaattttctgcccaaatggctgggattgttcattttttgtttgccttacaacgagagtgacagtacgcgcgggtagttaactcaacatctgaccggtcgataactcacttcacgccccgaagggggaagctgcctgacctacga ttcccgctatttcattcactgaccgggaggttcaaaatga 190 accggataagagagaaaagtgtcgacgtcggtccggttgatattgaccggcgcatccgccagctcgcccagtttttggtggatctgtttggcgattttgcgggtcttgccggtgtcggtgccgaaaaaaataccaatatttgccataacacacgctcctgttgaaaaagagatcccgcggggaaaatgcggtgaac atgtcagctattgcgaagagtgtgccagttttgctcacgggcaaaagctgcaccagaatgggtattaatgcaccagcctggcgctttttttcgcggcacgtcccctcgctaatgcccgtctggcgcggctttgacgctgataaggcgctgaataccgatctggatcaaggttttgtcgggttatcgtccaaaagg tgcactctttgcatggttataagtgcctgacatggtgtccgggcgaacgtcgccaggtggcacaaattgtcagaactacgacacgactaaccgaccgcaggagtgtgcgatgaccctgaatatgatgatggatgccagccgcgtcaggttgaacgtaaaaaagtcggtctgcgcaaagcacgtcgtcgtccg cagttctccaaacgttaattggtttctgcttcggcagaacgattggcgaaaaacccggtgcgaaccgggtttttttatggataaagatcgtgttatccacagcaatccattgattatctctctctttttcagcatttccagaatcccctcaccacaaagcccgcaaaatctggtaaactatcatccaatttctgcccaaatggctgggattgttcat tttttgtttgccttacaacgagagtgacagtacgcgcgggtagttaactcaacatctgaccggtcgataactcacttcacgccccgaagggggaagctgcctgaccctacgattcccgctatttcattcactgaccgggaggttcaaaatgacccagcgaaccgagtcgggtaataccgtctggcgcttcgatttatcccagcagtt caccgcgatgcagcggataagcgtggttctcagccgggcgaccgaggttgaacagacactccagcaggtgctgtgcgtattgcacaatgacgcctttttgcagcacggcatgatctgtctgtacgacagccagcaggcgattttgactattgaagcgttgcaggaagccgatcagcagttgatccccggcagctcgcaaatt cgctaccgtccgggtgaagggctggtcgggacggtgctttcgcaggggcaatcgttagtgctggcgcgtgtggctgacgatcagcgctttcttgaccgcctgggactgtatgattacaacctgccgtttatcgccgtgccgctgatagggccggatgcgcagacttttggcgtgctgacggcgcaaccgat ggcgcgttacgaagagcggttacccgcctgcacccgctttctggaaacggt 191 atggcgctgaagcacctgatcacgctctgcgcggcgtcgccgatggtcgccagccagctggcgcgccacccgctgctgctggatgagctgctggatcccaacaccctctatcagccgacggcgaccgatgcctatcgcgcgacgagctgcgccagtacctgctgcgcgtgccggaagaggatgaagagcagca gctggaggcgttgcgccagtttaagcaggcgcagcagctgcatatcgcggcggcggatatcgctggtaccctgccggtgatgaaggtcagcgatcacttaacctggcttgccgaagcgatcctcgacgcggtggtgcagcaggcatgggggcagatggtcgctcgctacggccagccgacccacctgcacgatcg ccagggtcgcggcttcgccgtcgtcggctacggtaagcttggcggctgggagctgggctacagctccgatctcgatctggtgttcctccatgactgcccggcggaggtgatgaccgacggcgagcgggagattgacggccgtcagttctacctgcggctggcccagcggatcatgcacctgttcagcacccgcacctc gtccggtattctctacgaagtggacgcccggctgcgtccttctggcgcggcggggatgctggtcaccaccgccgacgcgtttgctgactatcagcagaacgaagcctggacgtgggaacatcaggcgctggtgcgcgcccgcgtggtctatggcgacccggcgctgcaggcgcgctttgacgccattcgtcg cgatatcctgaccaccccgcgggaggggatgaccctgcagaccgaggttcgcgagatgcgcgagaagatgcgcgcccaccttggcaacaaacatcccgatcgttttgatatcaaagccgatgccggcgggatcaccgatattgaatttattactcagtatctggtcctacgctatgccagtgacaagccgaagctgacccgctggtct gacaacgtgcgtattcttgagctgctggcgcagaacgacatcatggacgaggaggaggcgcgcgccttaacgcatgcgtacaccaccttgcgtgatgcgctccatcacctggccctgcaggagcagccgggacacacgtggcgccagaggccttcagccgggagcgtcagcaggtcagcgccagctggcagaagtggctgatgg cttaa 192 cgtaaggcgaccacccagctccgcgcgttgctgaacgacgctgaagccgttctgctggccgcggacaccgccgacgaggcgttattccgcaccgaggtcgtcggcgccaaactggccctgactgaatggctggtccagcgcggctggcgtccgttcctcaacgaggcaggagagaaaaaaatagccggatcgttcaaac ggtttgccgatattaacctctcgcggtggcggccgagctgcgcagcgccgtgcagcatctggcggttgaagatgccgccgaccagttgccgaagctgtcccgcgacatcgacagcgtccagctgctggcgggcgcctatggcgacgccgtcgcgccgtggctggagaactggcaggagcttcaccgtgcaatagca catgacgatcgcagcgtctttgaatatttccgtcgccaggcgctggctgccgagccgttctggctgcatagtggaaaacgataatttcaggccagggagcccttatggcgctgaagcacctgatcacgctctgcgcggcgtcgccgatggtcgccagccagctggcgcgccacccgctgctgctggatgagctgctgg atcccaacaccctctatcagccgacggcgaccgatgcctatcgcgacgagctgcgccagtacctgctgcgcgtgccggaagaggatgaagagcagcagctggaggcgttgcgccagtttaagcaggcgcagcagctgcatatcgcggcggcggatatcgctggtaccctgccggtgatgaaggtcagcgatcact taacctggcttgccgaagcgatcctcgacgcggtggtgcagcaggcatgggggcagatggtcgctcgctacggccagccgacccacctgcacgatcgccagggtcgcggcttcgccgtcgtcggctacggtaagcttggcggctgggagctgggctacagctccgatctcgatctggtgttcctccatgactgccc ggcggaggtgatgaccgacggcgagcgggagattgacggccgtcagttctacctgcggctggcccagcggatcatgcacctgttcagcacccgcacctcgtccggtattctctacgaagtggacgcccggctgcgtccttctggcgcggcggggatgctggtcaccaccgccgacgcgtttgctgactatcagcagaacga agcctggacgtgggaacatcaggcgctggtgcgcgcccgcgtggtctatggcgacccggcgctgcaggcgcgctttgacgccattcgtcgcgatatcctgaccaccccgcgggaggggatgaccctgcagaccgaggttcgcgagatgcgcgagaagatgcgcgcccaccttggcaacaaacatcccgatc gttttgatatcaaagccgatgccggcgggatcaccgatattgaatttattactcagtatctggtcctacgctatgccagtgacaagccgaagctgacccgctggtctgacaacgtgcgtattcttgagctgctggcgcagaacgacatcatggacgaggaggaggcgcgcgccttaacgcatgcgtacaccaccttgcgtgatgcg ctccatcacctggccctgcaggagcagccgggacacgtggcgccagaggccttcagccgggagcgtcagcaggtcagcgccagctggcagaagtggctgatggcttaactataaaatcgggtgtgctattatcgcgcgcaaaagtttgcgtctcgcaggagagtcgcaggagagtcatgaaagtaacgctgccggagtttgaacgtgcagg agtgttggtggtgggtgatgtgtgctggaccgctactggtacggccccaccagtcgtatttccccggaagccccggtgccggtggtgaaggtggaaaatatcgaagaacgtcctggcggcgcggcaaacgtagcgaatgaacatcgcctccctgggggcaacgtcgcgcctggtgggattgaccgggattgatgac gctgcccgcgcgctgagccaggcgctggccaatgtgaatgtgaagtgcgacttcgtctccgtcccgactcacccgaccatcaccaagctgcgggtgctgtcgcgcaatcagcagctgatccgcctcgactttgaagagggcttctccggcgtggatccgcagccgatgcatgagcgcattcagcaggcgctgg gagccattggcgcactgg 193 atgaccctgaatatgatgctagaagcgtcaggtaccggtcatgattcaccgtgcgattctcggttccctggagcgcttcattggcatcctgaccgaagagttcgctggcttcttcccaacctggattgcaccagtgcaggtagtggtcatgaatattaccgattctcaggctgaatacgttaacgaattgacgcgtaaactacaaaatgcggg cattcgtgtaaaagcagacttgagaaatgagaagattggctttaaaatccgcgagcacactttacgtcgtgtcccgtatatgttggtctgtggcgacaaagaagtcgaagccggcaaagtggccgtgcgcacccgtcgcgggaaagacctcggcagcatggacgtaagtgaagtgattgagaagctgcaacaagaga ttcgcagccgcagtcttcaacaactggaggaataaggtattaaaggcggaaaacgagttcaaacggcacgtccgaatcgtatcaatggcgagattcgcgccctggaagttcgcgccattgagctggcttcccgaccgcagggcggcacctgcctgaccctgcgtttcccgctgtttaacaccctgaccgggaggtgaag catga 194 ggccgtcgcccagcgtcggcgtccccaacagcagggccgggtaggccagcaggtccgccagcgtggcgcggttaatattgaccggggcggcggcggcctcccccagctgcttgtggatcattttcgcgatcttgcgggttttaccggtatcggtaccaaagaaaatgccaatgttcgccatagtacgctcctgt cggaatggtgttgaaaaaaggaatgacgacagaggtattgcgaaggctgtgccaggttgccctgcaccgcgacggcccatccctgccccatcaggatcgcttcgcatcacgatgccgcgcgccaaaggcgcacccggcggggcgaaaggtaaaaatccgtgaattttccccctgtcggatcaatgtttcgcg tggtcgttccgataagggcgcacactttgcatggttatccgggttcggcttaccccgccgcgttttgcgcacggtgtcggacaatttgtcataactgcgacacaggagtttgcgatgaccctgaatatgatgctagaagcgtcaggtaccggtcatgattcaccgtgcgattctcggttccctggagcgcttcattggcatcctga ccgaagagttcgctggcttcttcccaacctggattgcaccagtgcaggtagtggtcatgaatattaccgattctcaggctgaatacgttaacgaattgacgcgtaaactacaaaatgcgggcattcgtgtaaaagcagacttgagaaatgagaagattggctttaaaatccgcgagcacactttacgtcgtgtcccgtatatg ttggtctgtggcgacaaagaagtcgaagccggcaaagtggccgtgcgcacccgtcgcgggaaagacctcggcagcatggacgtaagtgaagtgattgagaagctgcaacaagagattcgcagccgcagtcttcaacaactggaggaataaggtattaaaggcggaaaacgagttcaaacggcacgtccgaatcgtatcaat ggcgagattcgcgccctggaagttcgcgccattgagctggcttcccgaccgcagggcggcacctgcctgaccctgcgtttcccgctgtttaacaccctgaccggaggtgaagcatgatccctgaatccgacccggacaccaccgtcagacgcttcgacctctctcagcagttcaccgccatgcagcggataagcgtggtg ctgagccgggccaccgaggccagcaaaacgctgcaggaggtgctcagcgtattacacaacgatgcctttatgcagcacgggatgatctgcctgtacgacagcgagcaggagatcctcagtatcgaagcgctgcagcaaaccggccagcagcccctccccggcagcacgcagatccgctatcgccccggcgagggactggt ggggaccgtgctggcccaggggcagtcgctggtgctgccccgggtcgccgacgatcagcgttttctcgaccgcctgagcctctacgattacgatctgccgtttcgccgtaccgttgatggggcccaacgcccggccaataggggtgctggcggcccagccgatggcgcgccaggaagagcggctgccggcctgcac ccgttttctcgaaaccgtc 195 atggcgctgaagcacctgatcacgctctgcgcggcgtcgccgatggtcgccagccagctggcgcgccacccgctgctgctggatgagctgctggatcccaacaccctctatcagccgacggcgaccgatgcctatcgcgcgacgagctgcgccagtacctgctgcgcgtgccggaagaggatgaagagcagca gctgcatatcgcggcggcggatatcgctggtaccctgccggtgatgaaggtcagcgatcacttaacctggcttgccgaagcgatcctcgacgcggtggtgcagcaggcatgggggcagatggtcgctcgctacggccagccgacccacctgcacgatcgccagggtcgcggcttcgccgtcgtcggctacgg taagcttggcggctgggagctgggctacagctccgatctcgatctggtgttcctccatgactgcccggcggaggtgatgaccgacggcgagcgggagattgacggccgtcagttctacctgcggctggcccagcggatcatgcacctgttcagcacccgcacctcgtccggtattctacgaagtggacgccggctgcgt ccttctggcgcggcggggatgctggtcaccaccgccgacgcgtttgctgactatcagcagaacgaagcctggacgtgggaacatcaggcgctggtgcgcccgcgtggtctatggcgacccggcgctgcaggcgcgctttgacgccattcgtcgcgatatcctgaccaccccgcgggaggggatgaccctgcaga ccgaggttcgcgagatgcgcgagaagatgcgcgcccaccttggcaacaaacatcccgatcgttttgatatcaaagccgatgccggcgggatcaccgatattgaatttattactcagtatctggtcctacgctatgccagtgacaagccgaagctgacccgctggtctgacaacgtgcgtattcttgagctgctggcgcagaac gacatcatggacgaggaggaggcgcgcgccttaacgcatgcgtacaccaccttgcgtgatgcgctccatcacctggccctgcaggagcagccgggacacgtggcgccagaggccttcagccgggagcgtcagcaggtcagcgccagctggcagaagtggctgatggcttaa 196 cgtaaggcgaccacccagctccgcgcgttgctgaacgacgctgaagccgttctgctggccgcggacaccgccgacgaggcgttattccgcaccgaggtcgtcggcgccaaactggccctgactgaatggctggtccagcgcggctggcgtccgttcctcaacgaggcaggagagaaaaaaatagccggatcgttcaaac ggtttgccgatattaacctctcgcggtggcggccgagctgcgcagcgccgtgcagcatctggcggttgaagatgccgccgaccagttgccgaagctgtcccgcgacatcgacagcgtccagctgctggcgggcgcctatggcgacgccgtcgcgccgtggctggagaactggcaggagcttcaccgtgcaatagca catgacgatcgcagcgtctttgaatatttccgtcgccaggcgctggctgccgagccgttctggctgcatagtggaaaacgataatttcaggccagggagcccttatggcgctgaagcacctgatcacgctctgcgcggcgtcgccgatggtcgccagccagctggcgcgccacccgctgctgctggatgagctgctgg atcccaacaccctctatcagccgacggcgaccgatgcctatcgcgacgagctgcgccagtacctgctgcgcgtgccggaagaggatgaagagcagcagctgcatatcgcggcggcggatatcgctggtaccctgccggtgatgaaggtcagcgatcacttaacctggcttgccgaagcgatcctcgacgcggtgg tgcagcaggcatgggggcagatggtcgctcgctacggccagccgacccacctgcacgatcgccagggtcgcggcttcgccgtcgtcggctacggtaagcttggcggctgggagctgggctacagctccgatctcgatctggtgttcctccatgactgcccggcggaggtgatgaccgacggcgagcgggagattgac ggccgtcagttctacctgcggctggcccagcggatcatgcacctgttcagcacccgcacctcgtccggtattctctacgaagtggacgcccggctgcgtccttctggcgcggcggggatgctggtcaccaccgccgacgcgttgctgactatcagcagcagaacgaagcctggacgtgggaacatcaggcgctggtgcgcg cccgcgtggtctatggcgacccggcgctgcaggcgcgctttgacgccattcgtcgcgatatcctgaccaccccgcgggaggggatgaccctgcagaccgaggttcgcgagatgcgcgagaagatgcgcgcccaccttggcaacaaacatcccgatcgttttgatatcaaagccgatgccggcgggatcaccgata ttgaatttattactcagtatctggtcctacgctatgccagtgacaagccgaagctgacccgctggtctgacaacgtgcgtattcttgagctgctggcgcagaacgacatcatggacgaggaggaggcgcgcgccttaacgcatgcgtacaccaccttgcgtgatgcgctccatcacctggccctgcaggagcagccgggacacgtgg cgccagaggccttcagccgggagcgtcagcaggtcagcgccagctggcagaagtggctgatggcttaactataaaatcgggtgtgctattatcgcgcgcaaagtttgcgtctcgcaggagagtcatgaaagtaacgctgccggagtttgaacgtgcaggagtgttggtggtgggtgatgtgtgctggaccg ctactggtacggccccaccagtcgtatttccccggaagccccggtgccggtggtgaaggtggaaaaatatcgaagaacgtcctggcggcgcggcaaacgtagcgatgaacatcgcctccctgggggcaacgtcgcgcctggtgggattgaccgggattgatgacgctgcccgcgcgctgagccaggcgctggccaatgt gaatgtgaagtgcgacttcgtctccgtcccgactcacccgaccatcaccaagctgcgggtgctgtcgcgcaatcagcagctgatccgcctcgactttgaagagggcttctccggcgtggatccgcagccgatgcatgagcgcattcagcaggcgctgggagccattggcgcactgg 197 atgaccctgaatatgatgctcgacgccgtcctcgcagtaccattgcaaccgactttacagcaagaagtgattctggcacgcatggaacaaattcttgccagtcgggctttatccgatgacgaacgcgcacagcttttatatgagcgcggagtgttgtatgatagtctcggtctgagggcattagcgcgaaatgatttttcacaagcgctggcaatccgacccgatatgcctgaagtattcaattacttaggcatttacttaacgcaggcaggcaattttgatgctgcctatgaagcgtttgattctgtacttgagcttgatcgccattgagctggcttcccgaccgcagggcggcacctgcctgaccctgcgtttcccgctgtttaacaccctgaccggaggtgaagcatga 198 cccaacagcagggccgggtaggccagcaggtccgccagcgtggcgcggttaatattgaccggggcggcggcggcctcccccagctgcttgtggatcattttcgcgatcttgcgggttttaccggtatcggtaccaaagaaaatgccaatgttcgccatagtacgctcctgtcggaatggtgttgaaaaaaggaatgac gacagaggtattgcgaaggctgtgccaggttgccctgcaccgcgacggcccatccctgccccatcaggatcgcttcgcatcacgatgccgcgcgccaaaggcgcacccggcggggcgaaaggtaaaaatccgtgaattttccccctgtcggatcaatgttcgcgtggtcgttccgataagggcgcacactttg catggttatccggttcggcttaccccgccgcgttttgcgcacggtgtcggacaatttgtcataactgcgacacaggagtttgcgatgaccctgaatatgatgctcgacgccgtcctcgcagtaccattgcaaccgactttacagcaagaagtgattctggcacgcatggaacaaattcttgccagtcgggctttatccgat gacgaacgcgcacagctttttatgagcgcggagtgttgtatgatagtctcggtctgagggcattagcgcgaaatgatttttcacaagcgctggcaatccgacccgatatgcctgaagtattcaattacttaggcatttacttaacgcaggcaggcaattttgatgctgcctatgaagcgtttgattctgtacttgagcttgatcgcca ttgagctggcttcccgaccgcagggcggcacctgcctgaccctgcgtttcccgctgtttaacaccctgaccgggaggtgaagcatgatccctgaatccgacccggacaccaccgtcagacgcttcgacctctctcagcagttcaccgccatgcagcggataagcgtggtgctgagccgggccaccgaggccagcaaaacgctgca ggaggtgctcagcgtattacacaacgatgcctttatgcagcacgggatgatctgcctgtacgacagcgagcaggagatcctcagtatcgaagcgctgcagcaaaccggccagcagcccctccccggcagcacgcagatccgctatcgccccggcgagggactggtggggaccgtgctggcccaggggcagtcgctgg tgctgccccgggtcgccgacgatcagcgttttctcgaccgcctgagcctctacgattacgatctgccgtttatcgccgtaccgttgatggggcccaacgcccggccaataggggtgctggcggcccagccgatggcgcgccaggaagagcggctgccggcctgcacccgttttctcgaaaccgtc 199 atggcgctgaagcacctgatcacgctctgcgcggcgtcgccgatggtcgccagccagctggcgcgccacccgctgctgctggatgagctgctggatcccaacaccctctatcagccgacggcgaccgatgcctatcgcgcgacgagctgcgccagtacctgctgcgcgtgccggaagaggatgaagagcagca gctgcatatcgcggcggcggatatcgctggtaccctgccggtgatgaaggtcagcgatcacttaacctggcttgccgaagcgatcctcgacgcggtggtgcagcaggcatgggggcagatggtcgctcgctacggccagccgacccacctgcacgatcgccagggtcgcggcttcgccgtcgtcggctacgg taagcttggcggctgggagctgggctacagctccgatctcgatctggtgttcctccatgactgcccggcggaggtgatgaccgacggcgagcgggagattgacggccgtcagttctacctgcggctggcccagcggatcatgcacctgttcagcacccgcacctcgtccggtattctacgaagtggacgccggctgcgt ccttctggcgcggcggggatgctggtcaccaccgccgacgcgtttgctgactatcagcagaacgaagcctggacgtgggaacatcaggcgctggtgcgcccgcgtggtctatggcgacccggcgctgcaggcgcgctttgacgccattcgtcgcgatatcctgaccaccccgcgggaggggatgaccctgcaga ccgaggttcgcgagatgcgcgagaagatgcgcgcccaccttggcaacaaacatcccgatcgttttgatatcaaagccgatgccggcgggatcaccgatattgaatttattactcagtatctggtcctacgctatgccagtgacaagccgaagctgacccgctggtctgacaacgtgcgtattcttgagctgctggcgcagaac gacatcatggacgaggaggaggcgcgcgccttaacgcatgcgtacaccaccttgcgtgatgcgctccatcacctggccctgcaggagcagccgggacacgtggcgccagaggccttcagccgggagcgtcagcaggtcagcgccagctggcagaagtggctgatggcttaa 200 cgtaaggcgaccacccagctccgcgcgttgctgaacgacgctgaagccgttctgctggccgcggacaccgccgacgaggcgttattccgcaccgaggtcgtcggcgccaaactggccctgactgaatggctggtccagcgcggctggcgtccgttcctcaacgaggcaggagagaaaaaaatagccggatcgttcaaac ggtttgccgatattaacctctcgcggtggcggccgagctgcgcagcgccgtgcagcatctggcggttgaagatgccgccgaccagttgccgaagctgtcccgcgacatcgacagcgtccagctgctggcgggcgcctatggcgacgccgtcgcgccgtggctggagaactggcaggagcttcaccgtgcaatagca catgacgatcgcagcgtctttgaatatttccgtcgccaggcgctggctgccgagccgttctggctgcatagtggaaaacgataatttcaggccagggagcccttatggcgctgaagcacctgatcacgctctgcgcggcgtcgccgatggtcgccagccagctggcgcgccacccgctgctgctggatgagctgctgg atcccaacaccctctatcagccgacggcgaccgatgcctatcgcgacgagctgcgccagtacctgctgcgcgtgccggaagaggatgaagagcagcagctgcatatcgcggcggcggatatcgctggtaccctgccggtgatgaaggtcagcgatcacttaacctggcttgccgaagcgatcctcgacgcggtgg tgcagcaggcatgggggcagatggtcgctcgctacggccagccgacccacctgcacgatcgccagggtcgcggcttcgccgtcgtcggctacggtaagcttggcggctgggagctgggctacagctccgatctcgatctggtgttcctccatgactgcccggcggaggtgatgaccgacggcgagcgggagattgac ggccgtcagttctacctgcggctggcccagcggatcatgcacctgttcagcacccgcacctcgtccggtattctctacgaagtggacgcccggctgcgtccttctggcgcggcggggatgctggtcaccaccgccgacgcgttgctgactatcagcagcagaacgaagcctggacgtgggaacatcaggcgctggtgcgcg cccgcgtggtctatggcgacccggcgctgcaggcgcgctttgacgccattcgtcgcgatatcctgaccaccccgcgggaggggatgaccctgcagaccgaggttcgcgagatgcgcgagaagatgcgcgcccaccttggcaacaaacatcccgatcgttttgatatcaaagccgatgccggcgggatcaccgata ttgaatttattactcagtatctggtcctacgctatgccagtgacaagccgaagctgacccgctggtctgacaacgtgcgtattcttgagctgctggcgcagaacgacatcatggacgaggaggaggcgcgcgccttaacgcatgcgtacaccaccttgcgtgatgcgctccatcacctggccctgcaggagcagccgggacacgtgg cgccagaggccttcagccgggagcgtcagcaggtcagcgccagctggcagaagtggctgatggcttaactataaaatcgggtgtgctattatcgcgcgcaaagtttgcgtctcgcaggagagtcatgaaagtaacgctgccggagtttgaacgtgcaggagtgttggtggtgggtgatgtgtgctggaccg ctactggtacggccccaccagtcgtatttccccggaagccccggtgccggtggtgaaggtggaaaaatatcgaagaacgtcctggcggcgcggcaaacgtagcgatgaacatcgcctccctgggggcaacgtcgcgcctggtgggattgaccgggattgatgacgctgcccgcgcgctgagccaggcgctggccaatgt gaatgtgaagtgcgacttcgtctccgtcccgactcacccgaccatcaccaagctgcgggtgctgtcgcgcaatcagcagctgatccgcctcgactttgaagagggcttctccggcgtggatccgcagccgatgcatgagcgcattcagcaggcgctgggagccattggcgcactgg 201 atgaccctgaatatgatgctcgagctaaagttctcggctaatcgctgataacatttgacgcaatgcgcaataaaagggcatttgatgccctttttgcacctttcataccagaacctggctcatcagtgattttttttgtcataatcattgctgagacaggctctgaagagggcgtttatacaccaaaccattcgagcggtagcgcgacgg caagtcagcgttctcctttgcaatagcagggaagaggcgccagaaccgccagcgttgaagcagtttgaacgcgttcagtgtataatccgaaacttaatttcggtttggagccattgagctggcttcccgaccgcagggcggcacctgcctgaccctgcgtttcccgctgtttaacaccctgaccggaggtgaagcatga 202 ggccgtcgcccagcgtcggcgtccccaacagcagggccgggtaggccagcaggtccgccagcgtggcgcggttaatattgaccggggcggcggcggcctcccccagctgcttgtggatcattttcgcgatcttgcgggttttaccggtatcggtaccaaagaaaatgccaatgttcgccatagtacgctcctgt cggaatggtgttgaaaaaaggaatgacgacagaggtattgcgaaggctgtgccaggttgccctgcaccgcgacggcccatccctgccccatcaggatcgcttcgcatcacgatgccgcgcgccaaaggcgcacccggcggggcgaaaggtaaaaatccgtgaattttccccctgtcggatcaatgtttcgcg tggtcgttccgataagggcgcacactttgcatggttatccgggttcggcttaccccgccgcgttttgcgcacggtgtcggacaatttgtcataactgcgacacaggagttgcgatgaccctgaatatgatgctcgagctaaagttctcggctaatcgctgataacatttgacgcaatgcgcaataaaagggcatttgat gccctttttgcacgctttcataccagaacctggctcatcagtgattttttgtcataatcattgctgagacaggctctgaagagggcgtttatacaccaaaccattcgagcggtagcgcgacggcaagtcagcgttctcctttgcaatagcagggaagaggcgccagaaccgccagcgttgaagcagtttgaacgcgttcagtgtataatcc gaaacttaatttcggtttggagccattgagctggcttcccgaccgcagggcggcacctgcctgaccctgcgtttcccgctgtttaacaccctgaccggaggtgaagcatgatccctgaatccgacccggacaccaccgtcagacgcttcgacctctctcagcagttcaccgccatgcagcggataagcgtggtgctgagccggggcca ccgaggccagcaaaacgctgcaggaggtgctcagcgtattacacaacgatgcctttatgcagcacgggatgatctgcctgtacgacgcgagcaggagatcctcagtatcgaagcgctgcagcaaaccggccagcagcccctccccggcagcacgcagatccgctatcgccccggcgagggactggtggggaccgtg ctggcccaggggcagtcgctggtgctgccccgggtcgccgacgatcagcgttttctcgaccgcctgagcctctacgattacgatctgccgttttcgccgtaccgttgatggggcccaacgcccggccaataggggtgctggcggcccagccgatggcgcgccaggaagagcggctgccggcctgcacccgttttctcga aaccgtc 203 atgaccctgaatatgatgctcgagcccgctgaccgaccagaacttccaccttggactcggctatacccttggcgtgacggcgcgcgataactgggactacatccccattccggtgatcttaccattggcgtcaataggttacggtccggcgactttccagatgacctatattcccggcacctacaataacggtaacgtttacttcgcctgggctcgtat acagttttaattcgctaagtcttagcaataaatgagataagcggtgtgtcttgtggaaaaacaaggactaaagcgttacccactaaaaaagatagcgacttttcactttttagcaaagttgcactggacaaaaaggtaccacaattggtgtactgatactcgacacagcattagtgtcgatttttcatataaaggtaatttgg ccattgagctggcttcccgaccgcagggcggcacctgcctgaccctgcgtttcccgctgtttaacaccctgaccgggaggtgaagcatga 204 ggccgtcgcccagcgtcggcgtccccaacagcagggccgggtaggccagcaggtccgccagcgtggcgcggttaatattgaccggggcggcggcggcctcccccagctgcttgtggatcattttcgcgatcttgcgggttttaccggtatcggtaccaaagaaaatgccaatgttcgccatagtacgctcctgt cggaatggtgttgaaaaaaggaatgacgacagaggtattgcgaaggctgtgccaggttgccctgcaccgcgacggcccatccctgccccatcaggatcgcttcgcatcacgatgccgcgcgccaaaggcgcacccggcggggcgaaaggtaaaaatccgtgaattttccccctgtcggatcaatgtttcgcg tggtcgttccgataagggcgcacactttgcatggttatccgggttcggcttaccccgccgcgttttgcgcacggtgtcggacaatttgtcataactgcgacacaggagtttgcgatgaccctgaatatgatgctcgagcccgctgaccgaccagaacttccaccttggactcggctatacccttggcgtgacggcgcgcgataact gggactacatccccattccggtgatcttaccattggcgtcaataggttacggtccggcgactttccagatgacctatattcccggcacctacaataacggtaacgtttacttcgcctgggctcgtatacagtttaattcgctaagtcttagcaataaatgagataagcggtgtgtcttgtggaaaaacaaggactaaagcgttacccact aaaaaagatagcgactttttcactttttagcaaagttgcactggacaaaaggtaccacaattggtgtactgatactcgacacagcattagtgtcgatttttcatataaaggtaattttggccattgagctggcttcccgaccgcagggcggcacctgcctgaccctgcgtttcccgctgtttaacaccctgaccggaggtgaagcatga tccctgaatccgacccggacaccaccgtcagacgcttcgacctctctcagcagttcaccgccatgcagcggataagcgtggtgctgagccgggccaccgaggccagcaaaacgctgcaggaggtgctcagcgtattacacaacgatgcctttatgcagcacgggatgatctgcctgtacgacagcgagcaggagatcctca gtatcgaagcgctgcagcaaaccggccagcagcccctccccggcagcacgcagatccgctatcgccccggcgagggactggtggggaccgtgctggcccaggggcagtcgctggtgctgccccgggtcgccgacgatcagcgttttctcgaccgcctgagcctctacgattacgatctgccgtttatcgccgta ccgttgatggggcccaacgcccggccaataggggtgctggcggcccagccgatggcgcgccaggaagagcggctgccggcctgcacccgttttctcgaaaccgtc 205 atggcgctgaagcacctgatcacgctctgcgcggcgtcgccgatggtcgccagccagctggcgcgccacccgctgctgctggatgagctgctggatcccaacaccctctatcagccgacggcgaccgatgcctatcgcgcgacgagctgcgccagtacctgctgcgcgtgccggaagaggatgaagagcagca gctgcatatcgcggcggcggatatcgctggtaccctgccggtgatgaaggtcagcgatcacttaacctggcttgccgaagcgatcctcgacgcggtggtgcagcaggcatgggggcagatggtcgctcgctacggccagccgacccacctgcacgatcgccagggtcgcggcttcgccgtcgtcggctacgg taagcttggcggctgggagctgggctacagctccgatctcgatctggtgttcctccatgactgcccggcggaggtgatgaccgacggcgagcgggagattgacggccgtcagttctacctgcggctggcccagcggatcatgcacctgttcagcacccgcacctcgtccggtattctacgaagtggacgccggctgcgt ccttctggcgcggcggggatgctggtcaccaccgccgacgcgtttgctgactatcagcagaacgaagcctggacgtgggaacatcaggcgctggtgcgcccgcgtggtctatggcgacccggcgctgcaggcgcgctttgacgccattcgtcgcgatatcctgaccaccccgcgggaggggatgaccctgcaga ccgaggttcgcgagatgcgcgagaagatgcgcgcccaccttggcaacaaacatcccgatcgttttgatatcaaagccgatgccggcgggatcaccgatattgaatttattactcagtatctggtcctacgctatgccagtgacaagccgaagctgacccgctggtctgacaacgtgcgtattcttgagctgctggcgcagaac gacatcatggacgaggaggaggcgcgcgccttaacgcatgcgtacaccaccttgcgtgatgcgctccatcacctggccctgcaggagcagccgggacacgtggcgccagaggccttcagccgggagcgtcagcaggtcagcgccagctggcagaagtggctgatggcttaa 206 cgtaaggcgaccacccagctccgcgcgttgctgaacgacgctgaagccgttctgctggccgcggacaccgccgacgaggcgttattccgcaccgaggtcgtcggcgccaaactggccctgactgaatggctggtccagcgcggctggcgtccgttcctcaacgaggcaggagagaaaaaaatagccggatcgttcaaac ggtttgccgatattaacctctcgcggtggcggccgagctgcgcagcgccgtgcagcatctggcggttgaagatgccgccgaccagttgccgaagctgtcccgcgacatcgacagcgtccagctgctggcgggcgcctatggcgacgccgtcgcgccgtggctggagaactggcaggagcttcaccgtgcaatagca catgacgatcgcagcgtctttgaatatttccgtcgccaggcgctggctgccgagccgttctggctgcatagtggaaaacgataatttcaggccagggagcccttatggcgctgaagcacctgatcacgctctgcgcggcgtcgccgatggtcgccagccagctggcgcgccacccgctgctgctggatgagctgctgg atcccaacaccctctatcagccgacggcgaccgatgcctatcgcgacgagctgcgccagtacctgctgcgcgtgccggaagaggatgaagagcagcagctgcatatcgcggcggcggatatcgctggtaccctgccggtgatgaaggtcagcgatcacttaacctggcttgccgaagcgatcctcgacgcggtgg tgcagcaggcatgggggcagatggtcgctcgctacggccagccgacccacctgcacgatcgccagggtcgcggcttcgccgtcgtcggctacggtaagcttggcggctgggagctgggctacagctccgatctcgatctggtgttcctccatgactgcccggcggaggtgatgaccgacggcgagcgggagattgac ggccgtcagttctacctgcggctggcccagcggatcatgcacctgttcagcacccgcacctcgtccggtattctctacgaagtggacgcccggctgcgtccttctggcgcggcggggatgctggtcaccaccgccgacgcgttgctgactatcagcagcagaacgaagcctggacgtgggaacatcaggcgctggtgcgcg cccgcgtggtctatggcgacccggcgctgcaggcgcgctttgacgccattcgtcgcgatatcctgaccaccccgcgggaggggatgaccctgcagaccgaggttcgcgagatgcgcgagaagatgcgcgcccaccttggcaacaaacatcccgatcgttttgatatcaaagccgatgccggcgggatcaccgata ttgaatttattactcagtatctggtcctacgctatgccagtgacaagccgaagctgacccgctggtctgacaacgtgcgtattcttgagctgctggcgcagaacgacatcatggacgaggaggaggcgcgcgccttaacgcatgcgtacaccaccttgcgtgatgcgctccatcacctggccctgcaggagcagccgggacacgtgg cgccagaggccttcagccgggagcgtcagcaggtcagcgccagctggcagaagtggctgatggcttaactataaaatcgggtgtgctattatcgcgcgcaaagtttgcgtctcgcaggagagtcatgaaagtaacgctgccggagtttgaacgtgcaggagtgttggtggtgggtgatgtgtgctggaccg ctactggtacggccccaccagtcgtatttccccggaagccccggtgccggtggtgaaggtggaaaaatatcgaagaacgtcctggcggcgcggcaaacgtagcgatgaacatcgcctccctgggggcaacgtcgcgcctggtgggattgaccgggattgatgacgctgcccgcgcgctgagccaggcgctggccaatgt gaatgtgaagtgcgacttcgtctccgtcccgactcacccgaccatcaccaagctgcgggtgctgtcgcgcaatcagcagctgatccgcctcgactttgaagagggcttctccggcgtggatccgcagccgatgcatgagcgcattcagcaggcgctgggagccattggcgcactgg 207 atgaccctgaatatgatgctagaagcgtcaggtaccggtcatgattcaccgtgcgattctcggttccctggagcgcttcattggcatcctgaccgaagagttcgctggcttcttcccaacctggattgcaccagtgcaggtagtggtcatgaatattaccgattctcaggctgaatacgttaacgaattgacgcgtaaactacaaaatgcggg cattcgtgtaaaagcagacttgagaaatgagaagattggctttaaaatccgcgagcacactttacgtcgtgtcccgtatatgttggtctgtggcgacaaagaagtcgaagccggcaaagtggccgtgcgcacccgtcgcgggaaagacctcggcagcatggacgtaagtgaagtgattgagaagctgcaacaagaga ttcgcagccgcagtcttcaacaactggaggaataaggtattaaaggcggaaaacgagttcaaacggcacgtccgaatcgtatcaatggcgagattcgcgccctggaagttcgcgccattgagctggcttcccgaccgcagggcggcacctgcctgaccctgcgtttcccgctgtttaacaccctgaccgggaggtgaag catga 208 ggccgtcgcccagcgtcggcgtccccaacagcagggccgggtaggccagcaggtccgccagcgtggcgcggttaatattgaccggggcggcggcggcctcccccagctgcttgtggatcattttcgcgatcttgcgggttttaccggtatcggtaccaaagaaaatgccaatgttcgccatagtacgctcctgt cggaatggtgttgaaaaaaggaatgacgacagaggtattgcgaaggctgtgccaggttgccctgcaccgcgacggcccatccctgccccatcaggatcgcttcgcatcacgatgccgcgcgccaaaggcgcacccggcggggcgaaaggtaaaaatccgtgaattttccccctgtcggatcaatgtttcgcg tggtcgttccgataagggcgcacactttgcatggttatccgggttcggcttaccccgccgcgttttgcgcacggtgtcggacaatttgtcataactgcgacacaggagtttgcgatgaccctgaatatgatgctagaagcgtcaggtaccggtcatgattcaccgtgcgattctcggttccctggagcgcttcattggcatcctga ccgaagagttcgctggcttcttcccaacctggattgcaccagtgcaggtagtggtcatgaatattaccgattctcaggctgaatacgttaacgaattgacgcgtaaactacaaaatgcgggcattcgtgtaaaagcagacttgagaaatgagaagattggctttaaaatccgcgagcacactttacgtcgtgtcccgtatatg ttggtctgtggcgacaaagaagtcgaagccggcaaagtggccgtgcgcacccgtcgcgggaaagacctcggcagcatggacgtaagtgaagtgattgagaagctgcaacaagagattcgcagccgcagtcttcaacaactggaggaataaggtattaaaggcggaaaacgagttcaaacggcacgtccgaatcgtatcaat ggcgagattcgcgccctggaagttcgcgccattgagctggcttcccgaccgcagggcggcacctgcctgaccctgcgtttcccgctgtttaacaccctgaccggaggtgaagcatgatccctgaatccgacccggacaccaccgtcagacgcttcgacctctctcagcagttcaccgccatgcagcggataagcgtggtg ctgagccgggccaccgaggccagcaaaacgctgcaggaggtgctcagcgtattacacaacgatgcctttatgcagcacgggatgatctgcctgtacgacagcgagcaggagatcctcagtatcgaagcgctgcagcaaaccggccagcagcccctccccggcagcacgcagatccgctatcgccccggcgagggactggt ggggaccgtgctggcccaggggcagtcgctggtgctgccccgggtcgccgacgatcagcgttttctcgaccgcctgagcctctacgattacgatctgccgtttcgccgtaccgttgatggggcccaacgcccggccaataggggtgctggcggcccagccgatggcgcgccaggaagagcggctgccggcctgcac ccgttttctcgaaaccgtc 209 atggcgctgaagcacctgatcacgctctgcgcggcgtcgccgatggtcgccagccagctggcgcgccacccgctgctgctggatgagctgctggatcccaacaccctctatcagccgacggcgaccgatgcctatcgcgcgacgagctgcgccagtacctgctgcgcgtgccggaagaggatgaagagcagca gctggaggcgttgcgccagtttaagcaggcgcagcagctgcatatcgcggcggcggatatcgctggtaccctgccggtgatgaaggtcagcgatcacttaacctggcttgccgaagcgatcctcgacgcggtggtgcagcaggcatgggggcagatggtcgctcgctacggccagccgacccacctgcacgatcg ccagggtcgcggcttcgccgtcgtcggctacggtaagcttggcggctgggagctgggctacagctccgatctcgatctggtgttcctccatgactgcccggcggaggtgatgaccgacggcgagcgggagattgacggccgtcagttctacctgcggctggcccagcggatcatgcacctgttcagcacccgcacctc gtccggtattctctacgaagtggacgcccggctgcgtccttctggcgcggcggggatgctggtcaccaccgccgacgcgtttgctgactatcagcagaacgaagcctggacgtgggaacatcaggcgctggtgcgcgcccgcgtggtctatggcgacccggcgctgcaggcgcgctttgacgccattcgtcg cgatatcctgaccaccccgcgggaggggatgaccctgcagaccgaggttcgcgagatgcgcgagaagatgcgcgcccaccttggcaacaaacatcccgatcgttttgatatcaaagccgatgccggcgggatcaccgatattgaatttattactcagtatctggtcctacgctatgccagtgacaagccgaagctgacccgctggtct gacaacgtgcgtattcttgagctgctggcgcagaacgacatcatggacgaggaggaggcgcgcgccttaacgcatgcgtacaccaccttgcgtgatgcgctccatcacctggccctgcaggagcagccgggacacacgtggcgccagaggccttcagccgggagcgtcagcaggtcagcgccagctggcagaagtggctgatgg cttaa 210 cgtaaggcgaccacccagctccgcgcgttgctgaacgacgctgaagccgttctgctggccgcggacaccgccgacgaggcgttattccgcaccgaggtcgtcggcgccaaactggccctgactgaatggctggtccagcgcggctggcgtccgttcctcaacgaggcaggagagaaaaaaatagccggatcgttcaaac ggtttgccgatattaacctctcgcggtggcggccgagctgcgcagcgccgtgcagcatctggcggttgaagatgccgccgaccagttgccgaagctgtcccgcgacatcgacagcgtccagctgctggcgggcgcctatggcgacgccgtcgcgccgtggctggagaactggcaggagcttcaccgtgcaatagca catgacgatcgcagcgtctttgaatatttccgtcgccaggcgctggctgccgagccgttctggctgcatagtggaaaacgataatttcaggccagggagcccttatggcgctgaagcacctgatcacgctctgcgcggcgtcgccgatggtcgccagccagctggcgcgccacccgctgctgctggatgagctgctgg atcccaacaccctctatcagccgacggcgaccgatgcctatcgcgacgagctgcgccagtacctgctgcgcgtgccggaagaggatgaagagcagcagctggaggcgttgcgccagtttaagcaggcgcagcagctgcatatcgcggcggcggatatcgctggtaccctgccggtgatgaaggtcagcgatcact taacctggcttgccgaagcgatcctcgacgcggtggtgcagcaggcatgggggcagatggtcgctcgctacggccagccgacccacctgcacgatcgccagggtcgcggcttcgccgtcgtcggctacggtaagcttggcggctgggagctgggctacagctccgatctcgatctggtgttcctccatgactgccc ggcggaggtgatgaccgacggcgagcgggagattgacggccgtcagttctacctgcggctggcccagcggatcatgcacctgttcagcacccgcacctcgtccggtattctctacgaagtggacgcccggctgcgtccttctggcgcggcggggatgctggtcaccaccgccgacgcgtttgctgactatcagcagaacga agcctggacgtgggaacatcaggcgctggtgcgcgcccgcgtggtctatggcgacccggcgctgcaggcgcgctttgacgccattcgtcgcgatatcctgaccaccccgcgggaggggatgaccctgcagaccgaggttcgcgagatgcgcgagaagatgcgcgcccaccttggcaacaaacatcccgatc gttttgatatcaaagccgatgccggcgggatcaccgatattgaatttattactcagtatctggtcctacgctatgccagtgacaagccgaagctgacccgctggtctgacaacgtgcgtattcttgagctgctggcgcagaacgacatcatggacgaggaggaggcgcgcgccttaacgcatgcgtacaccaccttgcgtgatgcg ctccatcacctggccctgcaggagcagccgggacacgtggcgccagaggccttcagccgggagcgtcagcaggtcagcgccagctggcagaagtggctgatggcttaactataaaatcgggtgtgctattatcgcgcgcaaaagtttgcgtctcgcaggagagtcgcaggagagtcatgaaagtaacgctgccggagtttgaacgtgcagg agtgttggtggtgggtgatgtgtgctggaccgctactggtacggccccaccagtcgtatttccccggaagccccggtgccggtggtgaaggtggaaaatatcgaagaacgtcctggcggcgcggcaaacgtagcgaatgaacatcgcctccctgggggcaacgtcgcgcctggtgggattgaccgggattgatgac gctgcccgcgcgctgagccaggcgctggccaatgtgaatgtgaagtgcgacttcgtctccgtcccgactcacccgaccatcaccaagctgcgggtgctgtcgcgcaatcagcagctgatccgcctcgactttgaagagggcttctccggcgtggatccgcagccgatgcatgagcgcattcagcaggcgctgg gagccattggcgcactgg 211 atggcgctcaaacagttaatccgtctgtgtgccgcctcgccgatggtcgcgacacaacttgcacgtcatcctttattgctcgatgaactgctcgacccgcgcacgctttaccagccgattgagccgggcgcttaccgcgacgaactgcgtcagtatctgatgcgggtgccaacagaagacgaagaacagcagct tgaagccgtgcgccagttcaaacaggcccagcatttgcgtatcgcagccggggatatttccggggcattgccggtgatgaaagtcagtgaccatttaacctaccttgccgaggccattctcgatgtcgtggtgcagcatgcgtgggaacaaatggtcgtaaaatacgggcagcccgcgcatcttcagcaccgtga ggggcgcggttttgccgtggtcggttacgggaaactcggtggctgggagctgggttatagctcagatctggatctggtcttcctgctcgattgcgcgccggaggtgatgacggacggcgaacgcagcatcgacggacgtcagttttatcttcggctggcgcagcgcattatgcacttattcagcacccggacatcgtca ggcattctttacgaggttgatccgcgtctgcgaccttccggcgcatccggcatgctggtcagtaccattgaagcgtttgcagattatcaggccaatgaagcctggacgtgggagcatcaggcgctggttcgcgcgcgcgtggtttacggggatccgcaactgacacagcaatttaacgccacgcgtcgcgacattctttgccgcca gcgcgatggcgacggcctgcgtaaggaggtccgtgaaatgcgcgagaaaatgtatgcccatctggggagtaaaaaagcccacgagtttgatctgaaagccgatccgggtggcatcacggatattgaattcattgcacaatacctggttctgcgtttcgcgcatgatgagccgaagctgacgcgctggtctgataac gtgcggatttttgaactgatggcacgatatgacatcatgccggaagaggaagcgcgccatctgacgcaggcttatgtgacgctgcgcgatgaaattcatcatctggcgttgcaggaacacagcgggaaagtggccgcggacagctttgctactgagcgcgcgcagatccgtgccagctgggcaaagtggctcggctga 212 cggtactggaacagaaatcggcggatgcgcaggaaatttgttatgacacggcctgtctgaagtgcaagttagtgcttacttcctggctggcaacctcaggctggacgccgtttattgatgataaatctgcgaagaaactggacgcttccttcaaacgttttgctgacatcatgctcggtcgtaccgcagcggatctgaaagaagcct ttgcgcagccactgacggaagaaggttatcgcgatcagctggcgcgcctgaaacgccagatcattaccttccatttgcttgccggtgcttaccctgaaaaagacgtcgatgcgtatattgccggctgggtggacctgcaacaggccatcgttcagcagcaacacgcctgggaggattcggcccgttctcacgcggt gatgatggatgctttctggttaaacgggcaacctcgttaactgactgactagcctgggcaaactgcccgggctttttttgcaaggaatctgatttcatggcgctcaaacagttaatccgtctgtgtgccgcctcgccgatggtcgcgacacaacttgcacgtcatcctttattgctcgatgaactgctcgacccgcgc acgctttaccagccgattgagccgggcgcttaccgcgacgaactgcgtcagtatctgatgcgggtgccaacagaagacgaagaacagcagcttgaagccgtgcgccagttcaaacaggcccagcatttgcgtatcgcagccggggatatttccggggcattgccggtgatgaaagtcagtgaccatttaacctaccttgccgagg ccattctcgatgtcgtggtgcagcatgcgtgggaacaaatggtcgtaaaatacgggcagcccgcgcatcttcagcaccgtgaggggcgcggttttgccgtggtcggttacgggaaactcggtggctgggagctgggttatagctcagatctggatctggtcttcctgctcgattgcgcgccggaggtgatg acggacggcgaacgcagcatcgacggacgtcagttttatcttcggctggcgcagcgcattatgcacttattcagcacccggacatcgtcaggcattctttacgaggttgatccgcgtctgcgaccttccggcgcatccggcatgctggtcagtaccattgaagcgtttgcagattatcaggccaatgaagcctggacgtgggagcatcagg cgctggttcgcgcgcgcgtggtttacggggatccgcaactgacacagcaatttaacgccacgcgtcgcgacattctttgccgccagcgcgatggcgacggcctgcgtaaggaggtccgtgaaatgcgcgagaaaatgtatgcccatctggggagtaaaaaagcccacgagtttgatctgaaagccgat ccgggtggcatcacggatattgaattcattgcacaatacctggttctgcgtttcgcgcatgatgagccgaagctgacgcgctggtctgataacgtgcggatttttgaactgatggcacgatatgacatcatgccggaagaggaagcgcgccatctgacgcaggcttatgtgacgctgcgcgatgaaattcatcatctggcgttg caggaacacagcgggaaagtggccgcggacagctttgctactgagcgcgcgcagatccgtgccagctgggcaaagtggctcggctgagggtttttattcggctaacaggcgcttgtgatattatccggcgcattgtatttacccgatttgatttatctgttttggagtcttgggatgaaagtgactttgcctgattttcacc gcgcaggtgtgctggttgtcggtgacgtaatgttagaccgttactggtatggcccgaccaatcgtatttctccggaagctccggtgccggtggtgaaggtcagtaccattgaagagcggcctggcggtgcagctaacgtggcgatgaacatttcatctctgggcgcctcttcctgtctgatcggcctgaccggcgtagac gacgctgcgcgtgccctcagtgagcgtctggcagtgagcgtctggcagaagtgaaagttaactgcgatttcgtcgcactatccacacatcctaccatcaccaaactgcgaattttgtcccgtaaccagcaactgatccgcctcgactttgaggaaggttttgaaggcgttgatctcgagccgatgctgaccaaaataga 213 atgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggttgaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaac caccgtctgctggaattttttttcacaaagcgtagcgttattgaatcgcacattttaaactgttggccgctgtggaagcgaatattggtgaaaggtgcggttttaaggcctttttctttgactctctctgtcgttacaaagttaatatgcgcgccctccgtctctgaagctctcggtgaacattgttgcgaggcaggatgc gagctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgcgttatgcccgcccggaagcggcgttttcccgtccggggaatggcatggagctgcgccttatccagacgctgatcgcccatcatcgcggttctttagatctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtac agcaggttatcaccgggaggcttaaaatga 214 tgtttcgtctcgaggccgggcaactgagcggccccgttgaaaccgacctgggctggcatctgttgttgtgcgaacaaattcgcctgccgcaacccttgccgaaagccgaagccttaacgcgggtgcgtcagcaactgattgcccggcaacagaaacattatcagcgccagtggctgcaacaactgatcaacgcct gagcctgttctccttcttgttgatgcagacgggttaatgcccgttttgcacgaaaaatgcacataaattgcctgcgttgccttataacagcgcagggaaatcctgcctccggccttgtgccacaccgcgctttgcctggtttgtggtaaaaactggcccgctttgcatcctgatgcttaaaacaccccgttca gatcaacctttgggcagataagcccgcgaaaggcctgcaaattgcacggttattccgggtgagtatatgtgtgatttgggttccggcattgcgcaataaaggggagaaagacatgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggtt gaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaaccaccgtctgctggaattttttttcacaaagcgtagcgttattgaatcgcacattttaaactgttggccgctgtggaagcgaatattggtgaa aggtgcggttttaaggcctttttctttgactctctgtcgttacaaagttaatatgcgcgccctccgtctctgaagctctcggtgaacattgttgcgaggcaggatgcgagctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgcgttatgcccgcccggaagcggcgttttcccgtcc ggggaatggcatggagctgcgccttatccagacgctgatcgcccatcatcgcggttctttagatctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacagcaggttatcaccggaggcttaaaatgacccagttacctaccgcggcccggttatccggcgctttgatatgtctgcccagtttacggcg ctttatcgcatcagcgtggcgctgagtcaggaaagcaacaccgggcgcgcactggcggcgatcctcgaagtgcttcacgatcatgcatttatgcaatacggcatggtgtgtctgtttgataaagaacgcaatgcactctttgtggaatccctgcatggcatcgacggcgaaaggaaaaaagagaccgccatgtccg ttaccgcatgggggaaggcgtgatcggcgcggtgatgagccagcgtcaggcgctggtgttaccgcgcatttcagacgatcagcgttttctcgaccgcctgaatatttacgattacagcctgccgttgattggcgtgccgatccccggtgcggataatcagccatcgggcgtgctggtggcacagccgatggcg ttgcacgaagaccggctgactgccagtacgcggttttttagaaatggtc 215 atgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggttgaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaac caccgtctgctggaattttttttcacaaagcgtagcgttattgaatcgcacattttaaactgttggccgctgtggaagcgaatattggtgaaaggtgcggttttaaggcctttttctttgactctctctgtcgttacaaagttaatatgcgcgccctccgtctctgaagctctcggtgaacattgttgcgaggcaggatgc gagctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgcgttatgcccgcccggaagcggcgttttcccgtccggggaatggcatggagctgcgccttatccagacgctgatcgcccatcatcgcggttctttagatctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtac agcaggttatcaccgggaggcttaaaatga 216 tgtttcgtctcgaggccgggcaactgagcggccccgttgaaaccgacctgggctggcatctgttgttgtgcgaacaaattcgcctgccgcaacccttgccgaaagccgaagccttaacgcgggtgcgtcagcaactgattgcccggcaacagaaacattatcagcgccagtggctgcaacaactgatcaacgcct gagcctgttctccttcttgttgatgcagacgggttaatgcccgttttgcacgaaaaatgcacataaattgcctgcgttgccttataacagcgcagggaaatcctgcctccggccttgtgccacaccgcgctttgcctggtttgtggtaaaaactggcccgctttgcatcctgatgcttaaaacaccccgttca gatcaacctttgggcagataagcccgcgaaaggcctgcaaattgcacggttattccgggtgagtatatgtgtgatttgggttccggcattgcgcaataaaggggagaaagacatgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggtt gaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaaccaccgtctgctggaattttttttcacaaagcgtagcgttattgaatcgcacattttaaactgttggccgctgtggaagcgaatattggtgaa aggtgcggttttaaggcctttttctttgactctctgtcgttacaaagttaatatgcgcgccctccgtctctgaagctctcggtgaacattgttgcgaggcaggatgcgagctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgcgttatgcccgcccggaagcggcgttttcccgtcc ggggaatggcatggagctgcgccttatccagacgctgatcgcccatcatcgcggttctttagatctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacagcaggttatcaccggaggcttaaaatgacccagttacctaccgcggcccggttatccggcgctttgatatgtctgcccagtttacggcg ctttatcgcatcagcgtggcgctgagtcaggaaagcaacaccgggcgcgcactggcggcgatcctcgaagtgcttcacgatcatgcatttatgcaatacggcatggtgtgtctgtttgataaagaacgcaatgcactctttgtggaatccctgcatggcatcgacggcgaaaggaaaaaagagaccgccatgtccg ttaccgcatgggggaaggcgtgatcggcgcggtgatgagccagcgtcaggcgctggtgttaccgcgcatttcagacgatcagcgttttctcgaccgcctgaatatttacgattacagcctgccgttgattggcgtgccgatccccggtgcggataatcagccatcgggcgtgctggtggcacagccgatggcg ttgcacgaagaccggctgactgccagtacgcggttttttagaaatggtc 217 atgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggttgaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaac caccgtctgctggttaaaaacgtgaccacgagcattaataaacgccacgaaatgtggcgtttatttattcaaaaagtatcttctttcataaaaagtgctaaatgcagtagcagcaaaattgggataagtcccatggaatacggctgttttcgctgcaatttttaactttttcgtaaaaaaagatgtttctttgagcga acgatcaaaatatagcgttaaccggcaaaaaattattctcattagaaaatagtttgtgtaatacttgtaacgctacatggagattaacttaatctagagggttttataccgtctctgaagctctcggtgaacattgttgcgaggcaggatgcgagctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgcgttatgc ccgccgggaagcggcgttttcccgtccggggaatggcatggagctgcgccttatccagacgctgatcgcccatcatcgcggttctttagatctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacagcaggttatcaccggaggcttaaaatga 218 gtttcgtctcgaggccgggcaactgagcggccccgttgaaaccgacctgggctggcatctgttgttgtgcgaacaaattcgcctgccgcaacccttgccgaaagccgaagccttaacgcgggtgcgtcagcaactgattgcccggcaacagaaacattatcagcgccagtggctgcaacaactgatcaacgcctgag cctgttctccttcttgttgatgcagacgggttaatgcccgtttgcacgaaaaatgcacataaattgcctgcgttgccttataacagcgcagggaaatcctgcctccggccttgtgccacaccgcgctttgcctggtttgtggtaaaactggcccgctttgcatcctgatgcttaaaacacccgttcagat caacctttgggcagataagcccgcgaaaaggcctgcaaattgcacggttattccgggtgagtatatgtgtgatttgggttccggcattgcgcaataaaggggagaaagacatgagcatcacggcgttatcagcatcatttcctgagggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggttgaac aatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaaccaccgtctgctggttaaaaacgtgaccacgagcattaataaacgccacgaaatgtggcgtttatttattcaaaaagtatcttctttcataaa aagtgctaaatgcagtagcagcaaaattgggataagtcccatggaatacggctgttttcgctgcaatttttaactttttcgtaaaaaaagatgtttctttgagcgaacgatcaaaatatagcgttaaccggcaaaaaattattctcattagaaaatagttgtgtaatacttgtaacgctacatggagattaacttaatctagagggtt ttataccgtctctgaagctctcggtgaacattgttgcgaggcaggatgcgagctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgcgttatgcccgcccggaagcggcgttttcccgtccggggaatggcatggagctgcgccttatccagacgctgatcgcccatcatcgcggttctttagatctctc ggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacagcaggttatcaccggaggcttaaaatgacccagttacctaccgcgggcccggttatccggcgctttgatatgtctgcccagtttacggcgctttatcgcatcagcgtggcgctgagtcaggaaagcaacaccgggcgcgcactggcggc gatcctcgaagtgcttcacgatcatgcatttatgcaatacggcatggtgtgtctgtttgataaagaacgcaatgcactctttgtggaatccctgcatggcatcgacggcgaaaggaaaaaagagacccgccatgtccgttaccgcatgggggaaggcgtgatcggcgcggtgatgagccagcgtcaggcgctggtg ttaccgcgcatttcagacgatcagcgttttctcgaccgcctgaatatttacgattacagcctgccgttgattggcgtgccgatccccggtgcggataatcagccatcgggcgtgctggtggcacagccgatggcgttgcacgaagaccggctgactgccagtacgcggtttttagaaatggtcg 219 atgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggttgaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaac caccgtctgctggtgaacatcactgatgcacaagctacctatgtcgaagaattaactaaaaaactgcaagatgcaggcattcgcgttaaagccgacttgagaaatgagaagattggctttaaaattcgcgaacacacgctacgccgtgttccttatatgttagtttgtggcgataaagaggtcgaagcaggcaaagttgctgttc gtacccgccgcggcaaagacttaggaagcatggatgttagcgaagtcgttgacaaactgctggcggaaatccgcagcagaagtcttcatcaactggaggaataaagtattaaaggcggaaaacgagttcaaccggcgcgtcctaatcgcattaacaaagagattcgcgcgcaagaagttcgcctcacaggcgtcgatggcgag cagattggtattgtcagtctgaatgaagctcttgaaaaagctgaggaagcgggcgtcgatttagtagaaatcagtccgaatgccgagccgccagtttgtcgaatcccgtctctgaagctctcggtgaacattgttgcgaggcaggatgcgagctggttgtgttttgacattaccgataatgtgccgcgtgaacggggtg cgttatgcccgcccggaagcggcgttttcccgtccggggaatggcatggagctgcgccttatccagacgctgatcgcccatcatcgcggttctttagatctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacagcaggttatcaccggaggcttaaaatga 220 tgtttcgtctcgaggccgggcaactgagcggccccgttgaaaccgacctgggctggcatctgttgttgtgcgaacaaattcgcctgccgcaacccttgccgaaagccgaagccttaacgcgggtgcgtcagcaactgattgcccggcaacagaaacattatcagcgccagtggctgcaacaactgatcaacgcct gagcctgttctccttcttgttgatgcagacgggttaatgcccgttttgcacgaaaaatgcacataaattgcctgcgttgccttataacagcgcagggaaatcctgcctccggccttgtgccacaccgcgctttgcctggtttgtggtaaaaactggcccgctttgcatcctgatgcttaaaacaccccgttca gatcaacctttgggcagataagcccgcgaaaggcctgcaaattgcacggttattccgggtgagtatatgtgtgatttgggttccggcattgcgcaataaaggggagaaagacatgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggtt gaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaaccaccgtctgctggtgaacatcactgatgcacaagctacctatgtcgaagaattaactaaaaactgcaagatgcaggcattcgcgttaaagcc gacttgagaaatgagaagattggctttaaaattcgcgaacacacgctacgccgtgttccttatatgttagtttgtggcgataaagaggtcgaagcaggcaaagttgctgttcgtacccgccgcggcaaagacttaggaagcatggatgttagcgaagtcgttgacaaactgctggcggaaatccgcagcagaagtcttcatcaactgg aggaataaagtattaaaggcggaaaacgagttcaaccggcgcgtcctaatcgcattaacaaagagattcgcgcgcaagaagagttcgcctcacaggcgtcgatggcgagcagattggtattgtcagtctgaatgaagctcttgaaaaagctgaggaagcgggcgtcgatttagtagaaatcagtccgaatgccgagccg ccagtttgtcgaatcccgtctctgaagctctcggtgaacattgttgcgaggcaggatgcgagctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgcgttatgcccgcccggaagcggcgttttcccgtccggggaatggcatggagctgcgccttatccagacgctgatcgcccatcatcgcgg ttctttagatctctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacagcaggttatcaccggaggcttaaaatgacccagttacctaccgcggggcccggttatccggcgctttgatgtctgcccagtttacggcgctttatcgcatcagcgtggcgctgagtcaggaaagcaacaccgggcg cgcactggcggcgatcctcgaagtgcttcacgatcatgcatttatgcaatacggcatggtgtgtctgtttgataaagaacgcaatgcactctttgtggaatccctgcatggcatcgacggcgaaaggaaaaaagagacccgccatgtccgttaccgcatgggggaaggcgtgatcggcgcggtgatgagccagcgt caggcgctggtgttaccgcgcatttcagacgatcagcgttttctcgaccgcctgaatatttacgattacagcctgccgttgattggcgtgccgatccccggtgcggataatcagccatcgggcgtgctggtggcacagccgatggcgttgcacgaagaccggctgactgccagtacgcggtttttagaaatggtc 221 atgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggttgaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaac caccgtctgctggtgaacatcactgatgcacaagctacctatgtcgaagaattaactaaaaaactgcaagatgcaggcattcgcgttaaagccgacttgagaaatgagaagattggctttaaaattcgcgaacacacgctacgccgtgttccttatatgttagtttgtggcgataaagaggtcgaagcaggcaaagttgctgttc gtacccgccgcggcaaagacttaggaagcatggatgttagcgaagtcgttgacaaactgctggcggaaatccgcagcagaagtcttcatcaactggaggaataaagtattaaaggcggaaaacgagttcaaccggcgcgtcctaatcgcattaacaaagagattcgcgcgcaagaagttcgcctcacaggcgtcgatggcgag cagattggtattgtcagtctgaatgaagctcttgaaaaagctgaggaagcgggcgtcgatttagtagaaatcagtccgaatgccgagccgccagtttgtcgaatcccgtctctgaagctctcggtgaacattgttgcgaggcaggatgcgagctggttgtgttttgacattaccgataatgtgccgcgtgaacggggtg cgttatgcccgcccggaagcggcgttttcccgtccggggaatggcatggagctgcgccttatccagacgctgatcgcccatcatcgcggttctttagatctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacagcaggttatcaccggaggcttaaaatga 222 tgtttcgtctcgaggccgggcaactgagcggccccgttgaaaccgacctgggctggcatctgttgttgtgcgaacaaattcgcctgccgcaacccttgccgaaagccgaagccttaacgcgggtgcgtcagcaactgattgcccggcaacagaaacattatcagcgccagtggctgcaacaactgatcaacgcct gagcctgttctccttcttgttgatgcagacgggttaatgcccgttttgcacgaaaaatgcacataaattgcctgcgttgccttataacagcgcagggaaatcctgcctccggccttgtgccacaccgcgctttgcctggtttgtggtaaaaactggcccgctttgcatcctgatgcttaaaacaccccgttca gatcaacctttgggcagataagcccgcgaaaggcctgcaaattgcacggttattccgggtgagtatatgtgtgatttgggttccggcattgcgcaataaaggggagaaagacatgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggtt gaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaaccaccgtctgctggtgaacatcactgatgcacaagctacctatgtcgaagaattaactaaaaactgcaagatgcaggcattcgcgttaaagcc gacttgagaaatgagaagattggctttaaaattcgcgaacacacgctacgccgtgttccttatatgttagtttgtggcgataaagaggtcgaagcaggcaaagttgctgttcgtacccgccgcggcaaagacttaggaagcatggatgttagcgaagtcgttgacaaactgctggcggaaatccgcagcagaagtcttcatcaactgg aggaataaagtattaaaggcggaaaacgagttcaaccggcgcgtcctaatcgcattaacaaagagattcgcgcgcaagaagagttcgcctcacaggcgtcgatggcgagcagattggtattgtcagtctgaatgaagctcttgaaaaagctgaggaagcgggcgtcgatttagtagaaatcagtccgaatgccgagccg ccagtttgtcgaatcccgtctctgaagctctcggtgaacattgttgcgaggcaggatgcgagctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgcgttatgcccgcccggaagcggcgttttcccgtccggggaatggcatggagctgcgccttatccagacgctgatcgcccatcatcgcgg ttctttagatctctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacagcaggttatcaccggaggcttaaaatgacccagttacctaccgcggggcccggttatccggcgctttgatgtctgcccagtttacggcgctttatcgcatcagcgtggcgctgagtcaggaaagcaacaccgggcg cgcactggcggcgatcctcgaagtgcttcacgatcatgcatttatgcaatacggcatggtgtgtctgtttgataaagaacgcaatgcactctttgtggaatccctgcatggcatcgacggcgaaaggaaaaaagagacccgccatgtccgttaccgcatgggggaaggcgtgatcggcgcggtgatgagccagcgt caggcgctggtgttaccgcgcatttcagacgatcagcgttttctcgaccgcctgaatatttacgattacagcctgccgttgattggcgtgccgatccccggtgcggataatcagccatcgggcgtgctggtggcacagccgatggcgttgcacgaagaccggctgactgccagtacgcggtttttagaaatggtc 223 atggcgctcaaacagttaatccgtctgtgtgccgcctcgccgatggtcgcgacacaacttgcacgtcatcctttattgctcgatgaactgctcgacccgcgcacgctttaccagccgattgagccgggcgcttaccgcgacgaactgcgtcagtatctgatgcgggtgccaacagaagacgaagaacagcagct tgaagccgtgcgccagttcaaacaggcccagcatttgcgtatcgcagccggggatatttccggggcattgccggtgatgaaagtcagtgaccatttaacctaccttgccgaggccattctcgatgtcgtggtgcagcatgcgtgggaacaaatggtcgtaaaatacgggcagcccgcgcatcttcagcaccgtga ggggcgcggttttgccgtggtcggttacgggaaactcggtggctgggagctgggttatagctcagatctggatctggtcttcctgctcgattgcgcgccggaggtgatgacggacggcgaacgcagcatcgacggacgtcagttttatcttcggctggcgcagcgcattatgcacttattcagcacccggacatcgtca ggcattctttacgaggttgatccgcgtctgcgaccttccggcgcatccggcatgctggtcagtaccattgaagcgtttgcagattatcaggccaatgaagcctggacgtgggagcatcaggcgctggttcgcgcgcgcgtggtttacggggatccgcaactgacacagcaatttaacgccacgcgtcgcgacattctttgccgcca gcgcgatggcgacggcctgcgtaaggaggtccgtgaaatgcgcgagaaaatgtatgcccatctggggagtaaaaaagcccacgagtttgatctgaaagccgatccgggtggcatcacggatattgaattcattgcacaatacctggttctgcgtttcgcgcatgatgagccgaagctgacgcgctggtctgataac gtgcggatttttgaactgatggcacgatatgacatcatgccggaagaggaagcgcgccatctgacgcaggcttatgtgacgctgcgcgatgaaattcatcatctggcgttgcaggaacacagcgggaaagtggccgcggacagctttgctactgagcgcgcgcagatccgtgccagctgggcaaagtggctcggctga 224 cggtactggaacagaaatcggcggatgcgcaggaaatttgttatgacacggcctgtctgaagtgcaagttagtgcttacttcctggctggcaacctcaggctggacgccgtttattgatgataaatctgcgaagaaactggacgcttccttcaaacgttttgctgacatcatgctcggtcgtaccgcagcggatctgaaagaagcct ttgcgcagccactgacggaagaaggttatcgcgatcagctggcgcgcctgaaacgccagatcattaccttccatttgcttgccggtgcttaccctgaaaaagacgtcgatgcgtatattgccggctgggtggacctgcaacaggccatcgttcagcagcaacacgcctgggaggattcggcccgttctcacgcggt gatgatggatgctttctggttaaacgggcaacctcgttaactgactgactagcctgggcaaactgcccgggctttttttgcaaggaatctgatttcatggcgctcaaacagttaatccgtctgtgtgccgcctcgccgatggtcgcgacacaacttgcacgtcatcctttattgctcgatgaactgctcgacccgcgc acgctttaccagccgattgagccgggcgcttaccgcgacgaactgcgtcagtatctgatgcgggtgccaacagaagacgaagaacagcagcttgaagccgtgcgccagttcaaacaggcccagcatttgcgtatcgcagccggggatatttccggggcattgccggtgatgaaagtcagtgaccatttaacctaccttgccgagg ccattctcgatgtcgtggtgcagcatgcgtgggaacaaatggtcgtaaaatacgggcagcccgcgcatcttcagcaccgtgaggggcgcggttttgccgtggtcggttacgggaaactcggtggctgggagctgggttatagctcagatctggatctggtcttcctgctcgattgcgcgccggaggtgatg acggacggcgaacgcagcatcgacggacgtcagttttatcttcggctggcgcagcgcattatgcacttattcagcacccggacatcgtcaggcattctttacgaggttgatccgcgtctgcgaccttccggcgcatccggcatgctggtcagtaccattgaagcgtttgcagattatcaggccaatgaagcctggacgtgggagcatcagg cgctggttcgcgcgcgcgtggtttacggggatccgcaactgacacagcaatttaacgccacgcgtcgcgacattctttgccgccagcgcgatggcgacggcctgcgtaaggaggtccgtgaaatgcgcgagaaaatgtatgcccatctggggagtaaaaaagcccacgagtttgatctgaaagccgat ccgggtggcatcacggatattgaattcattgcacaatacctggttctgcgtttcgcgcatgatgagccgaagctgacgcgctggtctgataacgtgcggatttttgaactgatggcacgatatgacatcatgccggaagaggaagcgcgccatctgacgcaggcttatgtgacgctgcgcgatgaaattcatcatctggcgttg caggaacacagcgggaaagtggccgcggacagctttgctactgagcgcgcgcagatccgtgccagctgggcaaagtggctcggctgagggtttttattcggctaacaggcgcttgtgatattatccggcgcattgtatttacccgatttgatttatctgttttggagtcttgggatgaaagtgactttgcctgattttcacc gcgcaggtgtgctggttgtcggtgacgtaatgttagaccgttactggtatggcccgaccaatcgtatttctccggaagctccggtgccggtggtgaaggtcagtaccattgaagagcggcctggcggtgcagctaacgtggcgatgaacatttcatctctgggcgcctcttcctgtctgatcggcctgaccggcgtagac gacgctgcgcgtgccctcagtgagcgtctggcagtgagcgtctggcagaagtgaaagttaactgcgatttcgtcgcactatccacacatcctaccatcaccaaactgcgaattttgtcccgtaaccagcaactgatccgcctcgactttgaggaaggttttgaaggcgttgatctcgagccgatgctgaccaaaataga 225 atgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggttgaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaac caccgtctgctggtacagtagcgcctctcaaaaatagataaacggctcatgtacgtgggccgtttattttttctacccataatcgggaaccggtgttataatgccgcgccctcatattgtggggatttcttaatgacctatcctgggtcctaaagttgtagttgacattagcggagcactaacccgtctctgaagctctcggtgaacattgtt gcgaggcaggatgcgagctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgccgttatgcccgcccggaagcggcgttttcccgtccggggaatggcatggagctgcgccttatccagacgctgatcgcccatcatcgcggttctttagatctctcggtccgccctgatggcggcaccttgctgacgtt acgcctgccggtacagcaggttatcaccgggaggcttaaaatga 226 tgtttcgtctcgaggccgggcaactgagcggccccgttgaaaccgacctgggctggcatctgttgttgtgcgaacaaattcgcctgccgcaacccttgccgaaagccgaagccttaacgcgggtgcgtcagcaactgattgcccggcaacagaaacattatcagcgccagtggctgcaacaactgatcaacgcct gagcctgttctccttcttgttgatgcagacgggttaatgcccgttttgcacgaaaaatgcacataaattgcctgcgttgccttataacagcgcagggaaatcctgcctccggccttgtgccacaccgcgctttgcctggtttgtggtaaaaactggcccgctttgcatcctgatgcttaaaacaccccgttca gatcaacctttgggcagataagcccgcgaaaggcctgcaaattgcacggttattccgggtgagtatatgtgtgatttgggttccggcattgcgcaataaaggggagaaagacatgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggtt gaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaaccaccgtctgctggtacagtagcgcctctcaaaaatagataaacggctcatgtacgtgggccgtttattttttctacccataatcgggaaccggt gttataatgccgcgccctcatattgtggggatttcttaatgacctatcctgggtcctaaagttgtagttgacattagcggagcactaacccgtctctgaagctctcggtgaacattgttgcgaggcaggatgcgagctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgcgttatgcccgcccggaagcgg cgttttcccgtccggggaatggcatggagctgcgccttatccagacgctgatcgcccatcgcggttctttagatctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacagcaggttatcaccgggaggcttaaaatgacccagttacctaccgcgggcccggttatccggcgctttgatatgtctg cccagtttacggcgctttatcgcatcagcgtttatcgcatcagcgtggcgctgagtcaggaaagcaacaccgggcgcgccactggcggcgatcctcgaagtgcttcacgatcatgcatttatgcaatacggcatggtgtgtctgtttgataaagaacgcaatgcactctttgtggaatccctgcatggcatcgacggcgaaaggaaaaaagag acccgccatgtccgttaccgcatgggggaaggcgtgatcggcgcggtgatgagccagcgtcaggcgctggtgttaccgcgcatttcagacgatcagcgttttctcgaccgcctgaatatttacgattacagcctgccgccgttgattggcgtgccgatccccggtgcggataatcagccatcgggcgtgctggtgg cacagccgatggcgttgcacgaagaccggctgactgccagtacgcggtttttagaaatggtc 227 atggcgctcaaacagttaatccgtctgtgtgccgcctcgccgatggtcgcgacacaacttgcacgtcatcctttattgctcgatgaactgctcgacccgcgcacgctttaccagccgattgagccgggcgcttaccgcgacgaactgcgtcagtatctgatgcgggtgccaacagaagacgaagaacagcagct tgaagccgtgcgccagttcaaacaggcccagcatttgcgtatcgcagccggggatatttccggggcattgccggtgatgaaagtcagtgaccatttaacctaccttgccgaggccattctcgatgtcgtggtgcagcatgcgtgggaacaaatggtcgtaaaatacgggcagcccgcgcatcttcagcaccgtga ggggcgcggttttgccgtggtcggttacgggaaactcggtggctgggagctgggttatagctcagatctggatctggtcttcctgctcgattgcgcgccggaggtgatgacggacggcgaacgcagcatcgacggacgtcagttttatcttcggctggcgcagcgcattatgcacttattcagcacccggacatcgtca ggcattctttacgaggttgatccgcgtctgcgaccttccggcgcatccggcatgctggtcagtaccattgaagcgtttgcagattatcaggccaatgaagcctggacgtgggagcatcaggcgctggttcgcgcgcgcgtggtttacggggatccgcaactgacacagcaatttaacgccacgcgtcgcgacattctttgccgcca gcgcgatggcgacggcctgcgtaaggaggtccgtgaaatgcgcgagaaaatgtatgcccatctggggagtaaaaaagcccacgagtttgatctgaaagccgatccgggtggcatcacggatattgaattcattgcacaatacctggttctgcgtttcgcgcatgatgagccgaagctgacgcgctggtctgataac gtgcggatttttgaactgatggcacgatatgacatcatgccggaagaggaagcgcgccatctgacgcaggcttatgtgacgctgcgcgatgaaattcatcatctggcgttgcaggaacacagcgggaaagtggccgcggacagctttgctactgagcgcgcgcagatccgtgccagctgggcaaagtggctcggctga 228 cggtactggaacagaaatcggcggatgcgcaggaaatttgttatgacacggcctgtctgaagtgcaagttagtgcttacttcctggctggcaacctcaggctggacgccgtttattgatgataaatctgcgaagaaactggacgcttccttcaaacgttttgctgacatcatgctcggtcgtaccgcagcggatctgaaagaagcct ttgcgcagccactgacggaagaaggttatcgcgatcagctggcgcgcctgaaacgccagatcattaccttccatttgcttgccggtgcttaccctgaaaaagacgtcgatgcgtatattgccggctgggtggacctgcaacaggccatcgttcagcagcaacacgcctgggaggattcggcccgttctcacgcggt gatgatggatgctttctggttaaacgggcaacctcgttaactgactgactagcctgggcaaactgcccgggctttttttgcaaggaatctgatttcatggcgctcaaacagttaatccgtctgtgtgccgcctcgccgatggtcgcgacacaacttgcacgtcatcctttattgctcgatgaactgctcgacccgcgc acgctttaccagccgattgagccgggcgcttaccgcgacgaactgcgtcagtatctgatgcgggtgccaacagaagacgaagaacagcagcttgaagccgtgcgccagttcaaacaggcccagcatttgcgtatcgcagccggggatatttccggggcattgccggtgatgaaagtcagtgaccatttaacctaccttgccgagg ccattctcgatgtcgtggtgcagcatgcgtgggaacaaatggtcgtaaaatacgggcagcccgcgcatcttcagcaccgtgaggggcgcggttttgccgtggtcggttacgggaaactcggtggctgggagctgggttatagctcagatctggatctggtcttcctgctcgattgcgcgccggaggtgatg acggacggcgaacgcagcatcgacggacgtcagttttatcttcggctggcgcagcgcattatgcacttattcagcacccggacatcgtcaggcattctttacgaggttgatccgcgtctgcgaccttccggcgcatccggcatgctggtcagtaccattgaagcgtttgcagattatcaggccaatgaagcctggacgtgggagcatcagg cgctggttcgcgcgcgcgtggtttacggggatccgcaactgacacagcaatttaacgccacgcgtcgcgacattctttgccgccagcgcgatggcgacggcctgcgtaaggaggtccgtgaaatgcgcgagaaaatgtatgcccatctggggagtaaaaaagcccacgagtttgatctgaaagccgat ccgggtggcatcacggatattgaattcattgcacaatacctggttctgcgtttcgcgcatgatgagccgaagctgacgcgctggtctgataacgtgcggatttttgaactgatggcacgatatgacatcatgccggaagaggaagcgcgccatctgacgcaggcttatgtgacgctgcgcgatgaaattcatcatctggcgttg caggaacacagcgggaaagtggccgcggacagctttgctactgagcgcgcgcagatccgtgccagctgggcaaagtggctcggctgagggtttttattcggctaacaggcgcttgtgatattatccggcgcattgtatttacccgatttgatttatctgttttggagtcttgggatgaaagtgactttgcctgattttcacc gcgcaggtgtgctggttgtcggtgacgtaatgttagaccgttactggtatggcccgaccaatcgtatttctccggaagctccggtgccggtggtgaaggtcagtaccattgaagagcggcctggcggtgcagctaacgtggcgatgaacatttcatctctgggcgcctcttcctgtctgatcggcctgaccggcgtagac gacgctgcgcgtgccctcagtgagcgtctggcagtgagcgtctggcagaagtgaaagttaactgcgatttcgtcgcactatccacacatcctaccatcaccaaactgcgaattttgtcccgtaaccagcaactgatccgcctcgactttgaggaaggttttgaaggcgttgatctcgagccgatgctgaccaaaataga 229 atgagcatcacggcgttatcagctgaatatcactgactcacaagctacctatgtcgaagaattaactaaaaactgcaagatgcaggcattcgcgttaaagccgacttgagaaatgagaagaagattggctttaaaattcgcgaacacacgctacgccgtgttccttatgttagtttgtggcgataaagaggtcgaagcaggcaaagtt gctgttcgtactcgtcgcggcaaagacttaggaagcatggatgttagcgaagtcgttgacaaactgctggcggaaatccgcagcagaagtcatcaactggaggaataaagtattaaaggcggaaaacgagttcaaccggcgcgtcctaatcgcattaacaaagagattcgcgcgcaagaagttcgcctcaccggcgtcga tggcgagcagattggtattgtcagtctgaatgaagctcttgaaaaagctgaggaagcgggcgtcgatttagtagaaatcagtccgaatgccgagccgccagtttgtcgaatctctttagatctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacagcaggttatcaccggaggcttaaaatga 230 tgtttcgtctcgaagccgggcaactgagcagccccgttgaaaccgaactgggctggcatctgttgttgtgcgaacaaattcgcctgccgcaacccttgccgaaagccgaggccttaacgcgggtgcgtcagcaactgattgcccggcaacagaatcattatcagcgccagtggctgcaacaactgatcaacgcctga gcctgttctccttcttgttggtgcagacgggttaatgcccgtttgcacgaaaaatgcacataaactgccttcgctgccttataacagcgcatggaaatcctgcctcctgccttgtgccacgccgcgctttgcctggtttgtggtaaaactggcccgctttgcatcctgatgtttaaaacaccccgttcaga tcaacctttgggcagataagcccgcgaaaggcctgcaaattgcacggttattccgggtgagtatatgtgtgatttgggttccggcattgcgcaataaaggggagaaagacatgagcatcacggcgttatcagctgaatatcactgactcacaagctacctatgtcgaagaattaactaaaaaactgcaagatgcaggcattcgcgt taaagccgacttgagaaatgagaagattggctttaaaattcgcgaacacacgctacgccgtgttccttatgttagttgtggcgataaagaggtcgaagcaggcaaagttgctgttcgtactcgtcgcggcaaagacttaggaagcatggatgttagcgaagtcgttgacaaactgctggcggaaatccgcagcagaagtcat catcaactggaggaataaagtattaaaggcggaaaacgagttcaaccggcgcgtcctaatcgcattaacaaagagattcgcgcgcaagaagttcgcctcaccggcgtcgatggcgagcagattggtattgtcagtctgaatgaagctcttgaaaaagctgaggaagcgggcgtcgatttagtagaaatcagtccgaatgcc gagccgccagtttgtcgaatctctttagatctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacagcaggttatcaccggaggcttaaaatgacccagttacctaccgcggcccggttatccggcgctttgatatgtctgcccagtttacggcgctttatcgcatcagcgtggcgctgagt caggaaagcaataccgcgcgccactggcggcgatcctcgaagtgcttcacgatcatgcatttatgcaatacggcatggtgtgtctgttcgataaagaacgcaatgcactgtttgtggaatccctgcatggcatcgacggcgaaaggaaaaaagaaacccgccatgtccgttaccgcatgggggaaggcgtgatcgg cgcggtgatgagccagcgtcaggcgctggtgttaccgcgcatttcagacgatcagcgttttctcgaccgcctgaatatttacgattacagcctgccgctgattggtgtgccgatccccggtgcggataatcagcctgcgggtgtgctggtggcacagccgatggcgttgcacgaagaccggctggctgccagtac gcggtttttagaaatggtc 231 atgaccctgaatatgatgatggatgccggcggacatcatcgcgacaaacaatattaataccggcaaccaccggcaatttacgagactgcgcaggcatcctttctcccgtcaatttctgtcaaataaagtaaaagaggcagtctacttgaattaccccggctggttgagcgtttgttgaaaaaaagtaactgaaaaatccgtagaatagcg ccactctgatggttaattaattaacctattcaattaagaattatctggatgaatgtgccattaaatgcgcagcataatggtgcgttgtgcgggaaaactgcttttttttgaaagggttggtcagtagcggaaacaactcacttcacaccccgaagggggaagttgcctgaccctacgattcccgctatttcattcactgaccggaggttcaaaatga 232 accggatacgagagaaaagtgtctacatcggttcggttgatattgaccggcgcatccgccagcccgcccagtttctggtggatctgtttggcgattttgcgggtctgccggtgtcggtgccgaaaaaaataccaatatttgccataacacacgctcctgttgaaaaagagatcccgccgggaaatgcggtgaacgtg tctgatattgcgaagagtgtgccagttttggtcgcgggcaaaacctgcaccagtttggttattaatgcaccagtctggcgctttttttcgccgagtttctcctcgctaatgcccgccaggcgcggctttggcgctgatagcgcgctgaataccgatctggatcaaggttttgtcgggttatcagccaaaaaggtgcactct ttgcatggttatacgtgcctgacatgttgtccgggcgacaaacggcctggtggcacaaattgtcagaactacgacacgactaactgaccgcaggagtgtgcgatgaccctgaatatgatgatggatgccggcggacatcatcgcgacaaacaatattaataatccggcaaccacaccggcaatttacgagactgcgcaggcatcctttctcccgt caatttctgtcaaataaagtaaaagaggcagtctacttgaattacccccggctggttgagcgtttgttgaaaaaaagtaactgaaaaatccgtagaatagcgccactctgatggttaattaacctattcaattaagaattatctggatgaatgtgccattaaatgcgcagcataatggtgcgttgtgcgggaaaactgctttttttgaaag ggttggtcagtagcggaaacaactcacttcacaccccgaagggggaagttgcctgaccctacgattcccgctatttcattcactgaccggaggttcaaaatgacccagcgaaccgagtcgggtaataccgtctggcgcttcgatttgtcccagcagttcactgcgatgcagcgcataagcgtggtactcagccgggcgaccga ggtcgatcagacgctccagcaagtgctgtgcgtattgcacaatgacgcctttttgcagcacggcatgatctgtctgtacgacgcagccagcaggcgattttgaatattgaagcgttgcaggaagccgatcagcagttaatccccggcagctcgcaaatccgctatcgtccgggcgaagggctggtcgggacggtgctttc gcaggggccaatcattagtgctggcgcgcgttgctgacgatcagcgctttcttgaccggctcgggttgtatgattacaacctgccgtttatcgccgtgccgctgatagggccagatgcgcagactttcggtgtgctgacggcacaacccatggcgcgttacgaagagcgattacccgcctgcacccgctttctggaaacgg tc 233 atgaccctgaatatgatgatggatgccggccgtcctgtaataataaccggacaattcggactgattaaaaaagcgcccttgtggcgcttttttttattcccgcctccatttaaaataaaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgttttaacacgcgtttttttaacct tttattgaaagtcggtgcttctttgagcgaacgatcaaatttaagtggattcccatcaaaaaaatattctcaacctaaaaaagttgtgtaatacttgtaacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgcaactcacttcacaccccgaagggggaagttgcctgaccctacgatt cccgctatttcattcactgaccgggaggttcaaaatga 234 accggatacgagagaaaagtgtctacatcggttcggttgatattgaccggcgcatccgccagcccgcccagtttctggtggatctgtttggcgattttgcgggtctgccggtgtcggtgccgaaaaaaataccaatatttgccataacacacgctcctgttgaaaaagagatcccgccgggaaatgcggtgaacgtg tctgatattgcgaagagtgtgccagttttggtcgcgggcaaaacctgcaccagtttggttattaatgcaccagtctggcgctttttttcgccgagtttctcctcgctaatgcccgccaggcgcggctttggcgctgatagcgcgctgaataccgatctggatcaaggttttgtcgggttatcagccaaaaaggtgcactct ttgcatggttatacgtgcctgacatgttgtccgggcgacaaacggcctggtggcacaaattgtcagaactacgacacgactaactgaccgcaggagtgtgcgatgaccctgaatatgatgatggatgccggccgtcctgtaataataaccggacaattcggactgattaaaaaagcgcccttgtggcgcttttttttattcccg cctccatttaaaataaaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgttttaacacgcgttttttaaccttttattgaaagtcggtgcttctttgagcgaacgatcaaatttaagtggattcccatcaaaaaaatttctcaacctaaaaaagtttgtgtaatacttg taacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgcaactcacttcacaccccgaagggggaagttgcctgaccctacgattcccgctatttcattcactgaccggaggttcaaaatgacccagcgaaccgagtcgggtaataccgtctggcgcttcgatttgtcccagcagttcactgcga tgcagcgcataagcgtggtactcagccgggcgaccgaggtcgatcagacgctccagcaagtgctgtgcgtattgcacaatgacgcctttttgcagcacggcatgatctgtctgtacgacagccagcaggcgattttgaatattgaagcgttgcaggaagccgatcagcagttaatccccggcagctcgcaaatccgct atcgtccgggcgaagggctggtcgggacggtgctttcgcagggccaatcattagtgctggcgcgcgttgctgacgatcagcgctttcttgaccggctcgggttgtatgattacaacctgccgtttatcgccgtgccgctgatagggccagatgcgcagactttcggtgtgctgacggcacaacccatggcgcgttac gaagagcgattacccgcctgcacccgctttctggaaacggtc 235 atgaccctgaatatgatgatggatgccggccgtcctgtaataataaccggacaattcggactgattaaaaaagcgcccttgtggcgcttttttttattcccgcctccatttaaaataaaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgttttaacacgcgtttttttaacct tttattgaaagtcggtgcttctttgagcgaacgatcaaatttaagtggattcccatcaaaaaaatattctcaacctaaaaaagttgtgtaatacttgtaacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgcaactcacttcacaccccgaagggggaagttgcctgaccctacgatt cccgctatttcattcactgaccgggaggttcaaaatga 236 accggatacgagagaaaagtgtctacatcggttcggttgatattgaccggcgcatccgccagcccgcccagtttctggtggatctgtttggcgattttgcgggtctgccggtgtcggtgccgaaaaaaataccaatatttgccataacacacgctcctgttgaaaaagagatcccgccgggaaatgcggtgaacgtg tctgatattgcgaagagtgtgccagttttggtcgcgggcaaaacctgcaccagtttggttattaatgcaccagtctggcgctttttttcgccgagtttctcctcgctaatgcccgccaggcgcggctttggcgctgatagcgcgctgaataccgatctggatcaaggttttgtcgggttatcagccaaaaaggtgcactct ttgcatggttatacgtgcctgacatgttgtccgggcgacaaacggcctggtggcacaaattgtcagaactacgacacgactaactgaccgcaggagtgtgcgatgaccctgaatatgatgatggatgccggccgtcctgtaataataaccggacaattcggactgattaaaaaagcgcccttgtggcgcttttttttattcccg cctccatttaaaataaaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgttttaacacgcgttttttaaccttttattgaaagtcggtgcttctttgagcgaacgatcaaatttaagtggattcccatcaaaaaaatttctcaacctaaaaaagtttgtgtaatacttg taacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgcaactcacttcacaccccgaagggggaagttgcctgaccctacgattcccgctatttcattcactgaccggaggttcaaaatgacccagcgaaccgagtcgggtaataccgtctggcgcttcgatttgtcccagcagttcactgcga tgcagcgcataagcgtggtactcagccgggcgaccgaggtcgatcagacgctccagcaagtgctgtgcgtattgcacaatgacgcctttttgcagcacggcatgatctgtctgtacgacagccagcaggcgattttgaatattgaagcgttgcaggaagccgatcagcagttaatccccggcagctcgcaaatccgct atcgtccgggcgaagggctggtcgggacggtgctttcgcagggccaatcattagtgctggcgcgcgttgctgacgatcagcgctttcttgaccggctcgggttgtatgattacaacctgccgtttatcgccgtgccgctgatagggccagatgcgcagactttcggtgtgctgacggcacaacccatggcgcgttac gaagagcgattacccgcctgcacccgctttctggaaacggtc 237 atggcactgaaacacctcatttccctgtgtgccgcgtcgccgatggttgccagtcagctggcgcgctacccgatcctgcttgatgaattgctcgacccgaatacgctctatcaaccgacggcgatgaatgcctatcgcgcgatgagctgcgccaatacctgctgcgcgcgtgccggaagatgatgaagagcaacag cttgaggcgctgcggcagtttaagcaggcgcagttgctgcgcgtggcggcggcggatattgccggtacgttgccagtaatgaaagtgagcgatcacttaacctggctggcggaagcgattattgatgcggtggtgcagcaagcctggggggcagatggtggcgcgttatggccagccaacgcatctgcacgatcgcga agggcgcggttttgcggtggtcggttatggcaagctgggcggctgggagctgggttacagctccgatctggatctggtattcctgcacgactgcccgatggatgtgatgaccgatggcgagcgtgaaatcgatggtcgccagttctatttgcgtctcgcgcagcgcgtgatgcacctgtttagcacgcgc acgtcgtccggcatcctttatgaagttgatgcgcgtctgcgtccatctggcgctgcggggatgctggtcactactacggaatcgttcgccgattaccagcaaaacgaagcctggacgtgggaacatcaggcgctggcccgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcg ccgcgatattctgatgacgcctcgcgacggcgcaacgctgcaaaccgacgtgcgagaaatgcgcgagaaaatgcgcgcccatcttggcaacaagcataaagaccgcttcgatctgaaagccgatgaaggcggtatcaccgacatcgagtttatcgcccaatatctggtgctgcgctttgcccatgacaagccga aactgacgcgctggtcggataatgtgcgcattctcgaagggctggcgcaaaacggcatggaggagcaggaagcgcaggcattgacgctggcgtacaccacattgcgtgatgagctgcaccacctggcgctgcaagagttgccgggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttattaaaacca gctgggacaagtggctggtggaa 238 gcgcaaagcgagtgctcacttacgtgatctgttgacacaatctgaagcgaccataacttgccgtttcagcgaatacggcggtgtggagcgcacaatcagccctggcgaagctggtgctcaccgagtggctagtgacgcagggctggcgaaccttccttgatgaaaaagcgcaggccaaattcgccgactcctttaa acgctttgctgacatccatctgtcacgcagcgccgccgagctgaaaaaagccttgcccaaccgctgggcgacagctatcgcgaccagttgccgcgcctggcgcgtgatatcgactgcgcgttactgctggccgggcattacgatcgcgcgcgcgccgtggaatggctggaaaactggcaggggctt cagcacgccattgaaacgcgccagagtcgaaatcgaacatttccgtaataccgcgattacccaggagccgttctggttgcacagcggaaaacgttaacgaaaggatatttcgcatggcactgaaacacctcatttccctgtgtgccgcgtcgccgatggttgccagtcagctggcgcgctacccgatcctgcttgat gaattgctcgacccgaatacgctctatcaaccgacggcgatgaatgcctatcgcgatgagctgcgccaatacctgctgcgcgtgccggaagatgatgaagagcaacagcttgaggcgctgcggcagtttaagcaggcgcagttgctgcgcgtggcggcggcggatattgccggtacgttgccagtaatgaaagt gagcgatcacttaacctggctggcggaagcgattattgatgcggtggtgcagcaagcctgggggcagatggtggcgcgttatggccagccaacgcatctgcacgatcgcgaagggcgcggttttgcggtggtcggttatggcaagctgggcggctgggagctgggttacagctccgatctggatctggtattcctgcacgactg cccgatggatgtgatgaccgatggcgagcgtgaaatcgatggtcgccagttctatttgcgtctcgcgcagcgcgtgatgcacctgtttagcacgcgcacgtcgtccggcatcctttatgaagttgatgcgcgtctgcgtccatctggcgctgcggggatgctggtcactactacggaatcgtt cgccgattaccagcaaaacgaagcctggacgtgggaacatcaggcgctggcccgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatattctgatgacgcctcgcgacggcgcaacgctgcaaaccgacgtgcgagaaatgcgcgagaaaatgcgtg cccatcttggcaacaagcataaagaccgcttcgatctgaaagccgatgaaggcggtatcaccgacatcgagtttatcgcccaatatctggtgctgcgctttgcccatgacaagccgaaactgacgcgctggtcggataatgtgcgcattctcgaagggctggcgcaaaacggcatcatggaggcaggaagcgcaggcattgacgct ggcgtacaccacattgcgtgatgagctgcaccacctggcgctgcaagagttgccgggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttattaaaaccagctgggacaagtggctggtggaaccgtgcgccccggcgtaagtgtggtatcatcgcgcgcaaattttgtatctctcaggagacagg aatgaaagtgacgctgccagagtttaagcaagccggtgtaatggtggtgggtgatgtgatgctggatcgttactggtatggcccaaccagccgtatctctccggaagcgccagtcccggttgttaaagtcgataccattgaagagcgtcctggcggcgcggcaaacgtggcgatgaatatcgcctcactgggcgcca cggcgcgtctggttggcctgactggcattgacgatgcggcgcgcgcgctgagcaaagcgctggccgatgttaacgttaaatgtgacttcgtttctgttccgacgcatcccaccatcactaagctgcgcgtgctgtcgcgtaaccagcagctgattcgcctggactttgaagagggttttttgaaggagtc gatccgcaaccgatgcatgaacgcatcagccaggcgcttggtaatattggcgcgctggtgctgtcggatt 239 atgtttaacgatctgattggcgatgatgaaacggattcgccggaagatgcgctttctgagagctggcgcgaattgtggcaggatgcgttgcaggaggaggattccacgcccgtgctggcgcatctctcagaggacgatcgccgccgcgtggtggcgctgattgccgattttcgcaaagagttggataaacgcaccatt ggccgcgagggcggcaggtactcgatcacttaatgccgcatctgctcagcgatgtatgctcgcgcgcgacgatgcgccagtaccgctgtcacgcctgacgccgctgctcaccggaattattacccgcaccacttaccttgagctgctaagtgaatttcccggcgcactgaaacacctcatttccctgtgtgccgcgt cgccgatggttgccagtcagctggcgcgctacccgatcctgcttgaatgaattgctcgacccgaatacgctctatcaaccgacggcgatgaatgcctatcgcgatgagctgcgccaatacctgctgcgcgtgccggaagatgatgaagagcaacagcttgaggcgctgcggcagtttaagcaggcgcagttgct gcgcgtggcggcggcggatattgccggtacgttgccagtaatgaaagtgagcgatcacttaacctggctggcggaagcgattattgatgcggtggtgcagcaagcctggggggcagatggtggcgcgttatggccagccaacgcatctgcacgatcgcgcgaagggcgcggttttgcggtggtcggttatggcaagctggg cggctgggagctgggttacagctccgatctggatctggtattcctgcacgactgcccgatggatgtgatgaccgatggcgagcgtgaaatcgatggtcgccagttctatttgcgtctcgcgcagcgcgtgatgcacctgtttagcacgcgcacgtcgtccggcatcctttatgaagttgatgcgcgt ctgcgtccatctggcgctgcggggatgctggtcactactacggaatcgttcgccgattaccagcaaaaacgaagcctggacgtgggaacatcaggcgctggcccgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatattctgatgacgcctcgcgacggcgcaac gctgcaaaccgacgtgcgagaaatgcgcgagaaaatgcgtgcccatcttggcaacaagcataaagaccgcttcgatctgaaagccgatgaaggcggtatcaccgacatcgagtttatcgcccaatatctggtgctgcgctttgcccatgacaagccgaaactgacgcgctggtcggataatgtgcgcattctcga agggctggcgcaaaacggcatcatggaggcaggaagcgcaggcattgacgctggcgtacaccacattgcgtgatgagctgcaccacctggcgctgcaagagttgccgggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttattaaaaccagctgggacaagtggctggtggaaccgtgcgccccggc gtaa 240 atgaccctgaatatgatgatggatgccggccgtcctgtaataataaccggacaattcggactgattaaaaaagcgcccttgtggcgcttttttttattcccgcctccatttaaaataaaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgttttaacacgcgtttttttaacct tttattgaaagtcggtgcttctttgagcgaacgatcaaatttaagtggattcccatcaaaaaaatattctcaacctaaaaaagttgtgtaatacttgtaacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgcaactcacttcacaccccgaagggggaagttgcctgaccctacgatt cccgctatttcattcactgaccgggaggttcaaaatga 241 accggatacgagagaaaagtgtctacatcggttcggttgatattgaccggcgcatccgccagcccgcccagtttctggtggatctgtttggcgattttgcgggtctgccggtgtcggtgccgaaaaaaataccaatatttgccataacacacgctcctgttgaaaaagagatcccgccgggaaatgcggtgaacgtg tctgatattgcgaagagtgtgccagttttggtcgcgggcaaaacctgcaccagtttggttattaatgcaccagtctggcgctttttttcgccgagtttctcctcgctaatgcccgccaggcgcggctttggcgctgatagcgcgctgaataccgatctggatcaaggttttgtcgggttatcagccaaaaaggtgcactct ttgcatggttatacgtgcctgacatgttgtccgggcgacaaacggcctggtggcacaaattgtcagaactacgacacgactaactgaccgcaggagtgtgcgatgaccctgaatatgatgatggatgccggccgtcctgtaataataaccggacaattcggactgattaaaaaagcgcccttgtggcgcttttttttattcccg cctccatttaaaataaaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgttttaacacgcgttttttaaccttttattgaaagtcggtgcttctttgagcgaacgatcaaatttaagtggattcccatcaaaaaaatttctcaacctaaaaaagtttgtgtaatacttg taacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgcaactcacttcacaccccgaagggggaagttgcctgaccctacgattcccgctatttcattcactgaccggaggttcaaaatgacccagcgaaccgagtcgggtaataccgtctggcgcttcgatttgtcccagcagttcactgcga tgcagcgcataagcgtggtactcagccgggcgaccgaggtcgatcagacgctccagcaagtgctgtgcgtattgcacaatgacgcctttttgcagcacggcatgatctgtctgtacgacagccagcaggcgattttgaatattgaagcgttgcaggaagccgatcagcagttaatccccggcagctcgcaaatccgct atcgtccgggcgaagggctggtcgggacggtgctttcgcagggccaatcattagtgctggcgcgcgttgctgacgatcagcgctttcttgaccggctcgggttgtatgattacaacctgccgtttatcgccgtgccgctgatagggccagatgcgcagactttcggtgtgctgacggcacaacccatggcgcgttac gaagagcgattacccgcctgcacccgctttctggaaacggtc 242 gcgcaaagcgagtgctcacttacgtgatctgttgacacaatctgaagcgaccataacttgccgtttcagcgaatacggcggtgtggagcgcacaatcagccctggcgaagctggtgctcaccgagtggctagtgacgcagggctggcgaaccttccttgatgaaaaagcgcaggccaaattcgccgactcctttaa acgctttgctgacatccatctgtcacgcagcgccgccgagctgaaaaaagccttgcccaaccgctgggcgacagctatcgcgaccagttgccgcgcctggcgcgtgatatcgactgcgcgttactgctggccgggcattacgatcgcgcgcgcgccgtggaatggctggaaaactggcaggggctt cagcacgccattgaaacgcgccagagtcgaaatcgaacatttccgtaataccgcgattacccaggagccgttctggttgcacagcggaaaacgttaacgaaaggatatttcgcatgtttaacgatctgattggcgatgatgaaacggattcgccggaagatgcgctttctgagagctggcgcgaattgtggcaggatgc gttgcaggaggaggattccacgcccgtgctggcgcatctctcagaggacgatcgccgccgcgtggtggcgctgattgccgattttcgcaaagagttggataaacgcaccattggcccgcgagggcggcaggtactcgatcacttaatgccgcatctgctcagcgatgtatgctcgcgcgacgatgcgccagtaccg ctgtcacgcctgacgccgctgctcaccggaattattacccgcaccacttaccttgagctgctaagtgaatttcccggcgcactgaaacacctcatttccctgtgtgccgcgtcgccgatggttgccagtcagctggcgcgctacccgaatcctgcttgaattgctcgacccgaatacgctctatcaaccgacggcga tgaatgcctatcgcgatgagctgcgccaatacctgctgcgcgtgccggaagatgatgaagagcaacagcttgaggcgctgcggcagtttaagcaggcgcagttgctgcgcgtggcggcggcggatattgccggtacgttgccagtaatgaaagtgagcgatcacttaacctggctggcggaagcgattattgatg cggtggtgcagcaagcctggggggcagatggtggcgcgttatggccagccaacgcatctgcacgatcgcgcgaagggcgcggttttgcggtggtcggttatggcaagctgggcggctgggagctgggttacagctccgatctggatctggtattcctgcacgactgcccgatggatgtgatgaccgatggcgagcgtgaaat cgatggtcgccagttctatttgcgtctcgcgcagcgcgtgatgcacctgtttagcacgcgcgcacgtcgtccggcatcctttatgaagttgatgcgcgtctgcgtccatctggcgctgcggggatgctggtcactactacggaatcgttcgccgattaccagcaaaacgaagcctggacgtgggaacatca ggcgctggcccgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatattctgatgacgcctcgcgacggcgcaacgctgcaaaccgacgtgcgagaaatgcgcgagaaaatgcgtgcccatcttggcaacaagcataaagaccgcttcgatctgaaag ccgatgaaggcggtatcaccgacatcgagtttatcgcccaatatctggtgctgcgctttgcccatgacaagccgaaactgacgcgctggtcggataatgtgcgcattctcgaagggctggcgcaaaacggcatcatggaggagcaggaagcgcaggcattgacgctggcgtacaccacattgcgtgatgagctgcaccacctggc gctgcaagagttgccgggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttattaaaaccagctgggacaagtggctggtggaaccgtgcgccccggcgtaagtgtggtatcatcgcgcgcaaattttgtatctctcaggagacaggaatgaaagtgacgctgccagagtttaagcaagccggtgta atggtggtgggtgatgtgatgctggatcgttactggtatggcccaaccagccgtatctctccggaagcgccagtcccggttgttaaagtcgataccattgaagagcgtcctggcggcgcggcaaacgtggcgatgaatatcgcctcactgggcgccacggcgcgtctggttggcctgactggcattgacgatgc ggcgcgcgcgctgagcaaagcgctggccgatgttaacgttaaatgtgacttcgtttctgttccgacgcatcccaccatcactaagctgcgcgtgctgtcgcgtaaccagcagctgattcgcctggactttgaagagggttttgaaggagtcgatccgcaaccgatgcatgaacgcatcagccaggcgcttgg taatattggcgcgctggtgctgtcggatt 243 atgtttaacgatctgattggcgatgatgaaacggattcgccggaagatgcgctttctgagagctggcgcgaattgtggcaggatgcgttgcaggaggaggattccacgcccgtgctggcgcatctctcagaggacgatcgccgccgcgtggtggcgctgattgccgattttcgcaaagagttggataaacgcaccatt ggccgcgagggcggcaggtactcgatcacttaatgccgcatctgctcagcgatgtatgctcgcgcgcgacgatgcgccagtaccgctgtcacgcctgacgccgctgctcaccggaattattacccgcaccacttaccttgagctgctaagtgaatttcccggcgcactgaaacacctcatttccctgtgtgccgcgt cgccgatggttgccagtcagctggcgcgctacccgatcctgcttgaatgaattgctcgacccgaatacgctctatcaaccgacggcgatgaatgcctatcgcgatgagctgcgccaatacctgctgcgcgtgccggaagatgatgaagagcaacagcttgaggcgctgcggcagtttaagcaggcgcagttgct gcgcgtggcggcggcggatattgccggtacgttgccagtaatgaaagtgagcgatcacttaacctggctggcggaagcgattattgatgcggtggtgcagcaagcctggggggcagatggtggcgcgttatggccagccaacgcatctgcacgatcgcgcgaagggcgcggttttgcggtggtcggttatggcaagctggg cggctgggagctgggttacagctccgatctggatctggtattcctgcacgactgcccgatggatgtgatgaccgatggcgagcgtgaaatcgatggtcgccagttctatttgcgtctcgcgcagcgcgtgatgcacctgtttagcacgcgcacgtcgtccggcatcctttatgaagttgatgcgcgt ctgcgtccatctggcgctgcggggatgctggtcactactacggaatcgttcgccgattaccagcaaaaacgaagcctggacgtgggaacatcaggcgctggcccgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatattctgatgacgcctcgcgacggcgcaac gctgcaaaccgacgtgcgagaaatgcgcgagaaaatgcgtgcccatcttggcaacaagcataaagaccgcttcgatctgaaagccgatgaaggcggtatcaccgacatcgagtttatcgcccaatatctggtgctgcgctttgcccatgacaagccgaaactgacgcgctggtcggataatgtgcgcattctcga agggctggcgcaaaacggcatcatggaggcaggaagcgcaggcattgacgctggcgtacaccacattgcgtgatgagctgcaccacctggcgctgcaagagttgccgggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttattaaaaccagctgggacaagtggctggtggaaccgtgcgccccggc gtaa 244 gcgcaaagcgagtgctcacttacgtgatctgttgacacaatctgaagcgaccataacttgccgtttcagcgaatacggcggtgtggagcgcacaatcagccctggcgaagctggtgctcaccgagtggctagtgacgcagggctggcgaaccttccttgatgaaaaagcgcaggccaaattcgccgactcctttaa acgctttgctgacatccatctgtcacgcagcgccgccgagctgaaaaaagccttgcccaaccgctgggcgacagctatcgcgaccagttgccgcgcctggcgcgtgatatcgactgcgcgttactgctggccgggcattacgatcgcgcgcgcgccgtggaatggctggaaaactggcaggggctt cagcacgccattgaaacgcgccagagtcgaaatcgaacatttccgtaataccgcgattacccaggagccgttctggttgcacagcggaaaacgttaacgaaaggatatttcgcatgtttaacgatctgattggcgatgatgaaacggattcgccggaagatgcgctttctgagagctggcgcgaattgtggcaggatgc gttgcaggaggaggattccacgcccgtgctggcgcatctctcagaggacgatcgccgccgcgtggtggcgctgattgccgattttcgcaaagagttggataaacgcaccattggcccgcgagggcggcaggtactcgatcacttaatgccgcatctgctcagcgatgtatgctcgcgcgacgatgcgccagtaccg ctgtcacgcctgacgccgctgctcaccggaattattacccgcaccacttaccttgagctgctaagtgaatttcccggcgcactgaaacacctcatttccctgtgtgccgcgtcgccgatggttgccagtcagctggcgcgctacccgaatcctgcttgaattgctcgacccgaatacgctctatcaaccgacggcga tgaatgcctatcgcgatgagctgcgccaatacctgctgcgcgtgccggaagatgatgaagagcaacagcttgaggcgctgcggcagtttaagcaggcgcagttgctgcgcgtggcggcggcggatattgccggtacgttgccagtaatgaaagtgagcgatcacttaacctggctggcggaagcgattattgatg cggtggtgcagcaagcctggggggcagatggtggcgcgttatggccagccaacgcatctgcacgatcgcgcgaagggcgcggttttgcggtggtcggttatggcaagctgggcggctgggagctgggttacagctccgatctggatctggtattcctgcacgactgcccgatggatgtgatgaccgatggcgagcgtgaaat cgatggtcgccagttctatttgcgtctcgcgcagcgcgtgatgcacctgtttagcacgcgcgcacgtcgtccggcatcctttatgaagttgatgcgcgtctgcgtccatctggcgctgcggggatgctggtcactactacggaatcgttcgccgattaccagcaaaacgaagcctggacgtgggaacatca ggcgctggcccgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatattctgatgacgcctcgcgacggcgcaacgctgcaaaccgacgtgcgagaaatgcgcgagaaaatgcgtgcccatcttggcaacaagcataaagaccgcttcgatctgaaag ccgatgaaggcggtatcaccgacatcgagtttatcgcccaatatctggtgctgcgctttgcccatgacaagccgaaactgacgcgctggtcggataatgtgcgcattctcgaagggctggcgcaaaacggcatcatggaggagcaggaagcgcaggcattgacgctggcgtacaccacattgcgtgatgagctgcaccacctggc gctgcaagagttgccgggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttattaaaaccagctgggacaagtggctggtggaaccgtgcgccccggcgtaagtgtggtatcatcgcgcgcaaattttgtatctctcaggagacaggaatgaaagtgacgctgccagagtttaagcaagccggtgta atggtggtgggtgatgtgatgctggatcgttactggtatggcccaaccagccgtatctctccggaagcgccagtcccggttgttaaagtcgataccattgaagagcgtcctggcggcgcggcaaacgtggcgatgaatatcgcctcactgggcgccacggcgcgtctggttggcctgactggcattgacgatgc ggcgcgcgcgctgagcaaagcgctggccgatgttaacgttaaatgtgacttcgtttctgttccgacgcatcccaccatcactaagctgcgcgtgctgtcgcgtaaccagcagctgattcgcctggactttgaagagggttttgaaggagtcgatccgcaaccgatgcatgaacgcatcagccaggcgcttgg taatattggcgcgctggtgctgtcggatt 245 atgaccctgaatatgatgatggatgccggcggacatcatcgcgacaaacaatattaataccggcaaccaccggcaatttacgagactgcgcaggcatcctttctcccgtcaatttctgtcaaataaagtaaaagaggcagtctacttgaattaccccggctggttgagcgtttgttgaaaaaaagtaactgaaaaatccgtagaatagcg ccactctgatggttaattaattaacctattcaattaagaattatctggatgaatgtgccattaaatgcgcagcataatggtgcgttgtgcgggaaaactgcttttttttgaaagggttggtcagtagcggaaacaactcacttcacaccccgaagggggaagttgcctgaccctacgattcccgctatttcattcactgaccggaggttcaaaatga 246 accggatacgagagaaaagtgtctacatcggttcggttgatattgaccggcgcatccgccagcccgcccagtttctggtggatctgtttggcgattttgcgggtctgccggtgtcggtgccgaaaaaaataccaatatttgccataacacacgctcctgttgaaaaagagatcccgccgggaaatgcggtgaacgtg tctgatattgcgaagagtgtgccagttttggtcgcgggcaaaacctgcaccagtttggttattaatgcaccagtctggcgctttttttcgccgagtttctcctcgctaatgcccgccaggcgcggctttggcgctgatagcgcgctgaataccgatctggatcaaggttttgtcgggttatcagccaaaaaggtgcactct ttgcatggttatacgtgcctgacatgttgtccgggcgacaaacggcctggtggcacaaattgtcagaactacgacacgactaactgaccgcaggagtgtgcgatgaccctgaatatgatgatggatgccggcggacatcatcgcgacaaacaatattaataatccggcaaccacaccggcaatttacgagactgcgcaggcatcctttctcccgt caatttctgtcaaataaagtaaaagaggcagtctacttgaattacccccggctggttgagcgtttgttgaaaaaaagtaactgaaaaatccgtagaatagcgccactctgatggttaattaacctattcaattaagaattatctggatgaatgtgccattaaatgcgcagcataatggtgcgttgtgcgggaaaactgctttttttgaaag ggttggtcagtagcggaaacaactcacttcacaccccgaagggggaagttgcctgaccctacgattcccgctatttcattcactgaccggaggttcaaaatgacccagcgaaccgagtcgggtaataccgtctggcgcttcgatttgtcccagcagttcactgcgatgcagcgcataagcgtggtactcagccgggcgaccga ggtcgatcagacgctccagcaagtgctgtgcgtattgcacaatgacgcctttttgcagcacggcatgatctgtctgtacgacgcagccagcaggcgattttgaatattgaagcgttgcaggaagccgatcagcagttaatccccggcagctcgcaaatccgctatcgtccgggcgaagggctggtcgggacggtgctttc gcaggggccaatcattagtgctggcgcgcgttgctgacgatcagcgctttcttgaccggctcgggttgtatgattacaacctgccgtttatcgccgtgccgctgatagggccagatgcgcagactttcggtgtgctgacggcacaacccatggcgcgttacgaagagcgattacccgcctgcacccgctttctggaaacgg tc 247 atgaccctgaatatgatgatggatgccggccgtcctgtaataataaccggacaattcggactgattaaaaaagcgcccttgtggcgcttttttttattcccgcctccatttaaaataaaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgttttaacacgcgtttttttaacct tttattgaaagtcggtgcttctttgagcgaacgatcaaatttaagtggattcccatcaaaaaaatattctcaacctaaaaaagttgtgtaatacttgtaacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgcaactcacttcacaccccgaagggggaagttgcctgaccctacgatt cccgctatttcattcactgaccgggaggttcaaaatga 248 accggatacgagagaaaagtgtctacatcggttcggttgatattgaccggcgcatccgccagcccgcccagtttctggtggatctgtttggcgattttgcgggtctgccggtgtcggtgccgaaaaaaataccaatatttgccataacacacgctcctgttgaaaaagagatcccgccgggaaatgcggtgaacgtg tctgatattgcgaagagtgtgccagttttggtcgcgggcaaaacctgcaccagtttggttattaatgcaccagtctggcgctttttttcgccgagtttctcctcgctaatgcccgccaggcgcggctttggcgctgatagcgcgctgaataccgatctggatcaaggttttgtcgggttatcagccaaaaaggtgcactct ttgcatggttatacgtgcctgacatgttgtccgggcgacaaacggcctggtggcacaaattgtcagaactacgacacgactaactgaccgcaggagtgtgcgatgaccctgaatatgatgatggatgccggccgtcctgtaataataaccggacaattcggactgattaaaaaagcgcccttgtggcgcttttttttattcccg cctccatttaaaataaaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgttttaacacgcgttttttaaccttttattgaaagtcggtgcttctttgagcgaacgatcaaatttaagtggattcccatcaaaaaaatttctcaacctaaaaaagtttgtgtaatacttg taacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgcaactcacttcacaccccgaagggggaagttgcctgaccctacgattcccgctatttcattcactgaccggaggttcaaaatgacccagcgaaccgagtcgggtaataccgtctggcgcttcgatttgtcccagcagttcactgcga tgcagcgcataagcgtggtactcagccgggcgaccgaggtcgatcagacgctccagcaagtgctgtgcgtattgcacaatgacgcctttttgcagcacggcatgatctgtctgtacgacagccagcaggcgattttgaatattgaagcgttgcaggaagccgatcagcagttaatccccggcagctcgcaaatccgct atcgtccgggcgaagggctggtcgggacggtgctttcgcagggccaatcattagtgctggcgcgcgttgctgacgatcagcgctttcttgaccggctcgggttgtatgattacaacctgccgtttatcgccgtgccgctgatagggccagatgcgcagactttcggtgtgctgacggcacaacccatggcgcgttac gaagagcgattacccgcctgcacccgctttctggaaacggtc 249 atggcactgaaacacctcatttccctgtgtgccgcgtcgccgatggttgccagtcagctggcgcgctacccgatcctgcttgatgaattgctcgacccgaatacgctctatcaaccgacggcgatgaatgcctatcgcgcgatgagctgcgccaatacctgctgcgcgcgtgccggaagatgatgaagagcaacag cttgaggcgctgcggcagtttaagcaggcgcagttgctgcgcgtggcggcggcggatattgccggtacgttgccagtaatgaaagtgagcgatcacttaacctggctggcggaagcgattattgatgcggtggtgcagcaagcctggggggcagatggtggcgcgttatggccagccaacgcatctgcacgatcgcga agggcgcggttttgcggtggtcggttatggcaagctgggcggctgggagctgggttacagctccgatctggatctggtattcctgcacgactgcccgatggatgtgatgaccgatggcgagcgtgaaatcgatggtcgccagttctatttgcgtctcgcgcagcgcgtgatgcacctgtttagcacgcgc acgtcgtccggcatcctttatgaagttgatgcgcgtctgcgtccatctggcgctgcggggatgctggtcactactacggaatcgttcgccgattaccagcaaaacgaagcctggacgtgggaacatcaggcgctggcccgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcg ccgcgatattctgatgacgcctcgcgacggcgcaacgctgcaaaccgacgtgcgagaaatgcgcgagaaaatgcgcgcccatcttggcaacaagcataaagaccgcttcgatctgaaagccgatgaaggcggtatcaccgacatcgagtttatcgcccaatatctggtgctgcgctttgcccatgacaagccga aactgacgcgctggtcggataatgtgcgcattctcgaagggctggcgcaaaacggcatggaggagcaggaagcgcaggcattgacgctggcgtacaccacattgcgtgatgagctgcaccacctggcgctgcaagagttgccgggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttattaaaacca gctgggacaagtggctggtggaa 250 gcgcaaagcgagtgctcacttacgtgatctgttgacacaatctgaagcgaccataacttgccgtttcagcgaatacggcggtgtggagcgcacaatcagccctggcgaagctggtgctcaccgagtggctagtgacgcagggctggcgaaccttccttgatgaaaaagcgcaggccaaattcgccgactcctttaa acgctttgctgacatccatctgtcacgcagcgccgccgagctgaaaaaagccttgcccaaccgctgggcgacagctatcgcgaccagttgccgcgcctggcgcgtgatatcgactgcgcgttactgctggccgggcattacgatcgcgcgcgcgccgtggaatggctggaaaactggcaggggctt cagcacgccattgaaacgcgccagagtcgaaatcgaacatttccgtaataccgcgattacccaggagccgttctggttgcacagcggaaaacgttaacgaaaggatatttcgcatggcactgaaacacctcatttccctgtgtgccgcgtcgccgatggttgccagtcagctggcgcgctacccgatcctgcttgat gaattgctcgacccgaatacgctctatcaaccgacggcgatgaatgcctatcgcgatgagctgcgccaatacctgctgcgcgtgccggaagatgatgaagagcaacagcttgaggcgctgcggcagtttaagcaggcgcagttgctgcgcgtggcggcggcggatattgccggtacgttgccagtaatgaaagt gagcgatcacttaacctggctggcggaagcgattattgatgcggtggtgcagcaagcctgggggcagatggtggcgcgttatggccagccaacgcatctgcacgatcgcgaagggcgcggttttgcggtggtcggttatggcaagctgggcggctgggagctgggttacagctccgatctggatctggtattcctgcacgactg cccgatggatgtgatgaccgatggcgagcgtgaaatcgatggtcgccagttctatttgcgtctcgcgcagcgcgtgatgcacctgtttagcacgcgcacgtcgtccggcatcctttatgaagttgatgcgcgtctgcgtccatctggcgctgcggggatgctggtcactactacggaatcgtt cgccgattaccagcaaaacgaagcctggacgtgggaacatcaggcgctggcccgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatattctgatgacgcctcgcgacggcgcaacgctgcaaaccgacgtgcgagaaatgcgcgagaaaatgcgtg cccatcttggcaacaagcataaagaccgcttcgatctgaaagccgatgaaggcggtatcaccgacatcgagtttatcgcccaatatctggtgctgcgctttgcccatgacaagccgaaactgacgcgctggtcggataatgtgcgcattctcgaagggctggcgcaaaacggcatcatggaggcaggaagcgcaggcattgacgct ggcgtacaccacattgcgtgatgagctgcaccacctggcgctgcaagagttgccgggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttattaaaaccagctgggacaagtggctggtggaaccgtgcgccccggcgtaagtgtggtatcatcgcgcgcaaattttgtatctctcaggagacagg aatgaaagtgacgctgccagagtttaagcaagccggtgtaatggtggtgggtgatgtgatgctggatcgttactggtatggcccaaccagccgtatctctccggaagcgccagtcccggttgttaaagtcgataccattgaagagcgtcctggcggcgcggcaaacgtggcgatgaatatcgcctcactgggcgcca cggcgcgtctggttggcctgactggcattgacgatgcggcgcgcgcgctgagcaaagcgctggccgatgttaacgttaaatgtgacttcgtttctgttccgacgcatcccaccatcactaagctgcgcgtgctgtcgcgtaaccagcagctgattcgcctggactttgaagagggttttttgaaggagtc gatccgcaaccgatgcatgaacgcatcagccaggcgcttggtaatattggcgcgctggtgctgtcggatt 251 tttcgctgaaggtgtgaccatgggccatcaggtgctggtgcagctggaaagtgttgccatcactatcgtgtggtctggcgtggtggcctttattggttacaaactggcggacatgacggtaggcctgcgcgtaccggaagaacaagaacgtgaagggctggatgtaaacagccacggcgaaaacgcctataacgcc tga 252 tttcctttctgactctgcccgtccgggcgcactaacggcctgaaatactccctcttttcattcctggcacaacgattgcaatgtctgttgcgtgttagctgcggccattatcgaattcgactggagggggatctatgaagctggttaccgtggtgattaagccattcaaacttgaagacgtgcgtgaagcgctttcttctatt ggtattcaagggttgaccgtaactgaagtgaaaggctttggccgtcagaagggtcacgctgagctgtaccgcggtgcggaatatagcgttaatttcctgccgaaagtgaaaattgatgtggcgatcgctgacgatcaactcgatgaagtaatcgatgtgatcagcaaagcggcctacaccggaaaaattggcgacgg caaaattttcgttgctgagctgcaacgcgtgagctgcaacgcgtcattcgtattcgtaccggcgaagccgacgaagcggcactgtaatacaagacacacagtgatggggatcggtttcgctgaaggtgtgaccatgggccatcaggtgctggtgcagctggaaagtgttgccatcactatcgtgtggtctggcgtggtggcctttattgg ttacaaactggcggacatgacggtaggcctgcgcgtaccggaagaacaagaacgtgaagggctggatgtaaacagccacggcgaaaacgcctataacgcctgattgcgttgagttatctcctgagcataaaaaagcctccattcggaggcttttctttttttaagtttaaagcgcggttagttgcgattgcgcatgacgcctt cctgcacgctggacgcgaccagcacaccctcttgcgtatagaactcgccgcgcacaaaaccgcgagcgctggaggctgacgtgctttccacactgtagagcagccattcgttcatattaaacgggcgatggaaccacatggagtggtcaatggtggcaacctgcataccgcgctcaaggaagcccacgccgtgcggctgaagt gcaaccggcaggaagttaaagtctgaggcatatccaagcagatattgatgtacgcgaaaatcgtccggcaccgtgccgtttgcgcggatccatacctggcgggtgggatcggcaacgtggcctttcagcggtttatgaaactcaaccgggcggatctccagtggtttatcactaagaaacttctctttggcctgcgg 253 atgtttaacgatctgattggcgatgatgaaacggattcgccggaagatgcgctttctgagagctggcgcgaattgtggcaggatgcgttgcaggaggaggattccacgcccgtgctggcgcatctctcagaggacgatcgccgccgcgtggtggcgctgattgccgattttcgcaaagagttggataaacgcaccatt ggccgcgagggcggcaggtactcgatcacttaatgccgcatctgctcagcgatgtatgctcgcgcgcgacgatgcgccagtaccgctgtcacgcctgacgccgctgctcaccggaattattacccgcaccacttaccttgagctgctaagtgaatttcccggcgcactgaaacacctcatttccctgtgtgccgcgt cgccgatggttgccagtcagctggcgcgctacccgatcctgcttgaatgaattgctcgacccgaatacgctctatcaaccgacggcgatgaatgcctatcgcgatgagctgcgccaatacctgctgcgcgtgccggaagatgatgaagagcaacagcttgaggcgctgcggcagtttaagcaggcgcagttgct gcgcgtggcggcggcggatattgccggtacgttgccagtaatgaaagtgagcgatcacttaacctggctggcggaagcgattattgatgcggtggtgcagcaagcctggggggcagatggtggcgcgttatggccagccaacgcatctgcacgatcgcgcgaagggcgcggttttgcggtggtcggttatggcaagctggg cggctgggagctgggttacagctccgatctggatctggtattcctgcacgactgcccgatggatgtgatgaccgatggcgagcgtgaaatcgatggtcgccagttctatttgcgtctcgcgcagcgcgtgatgcacctgtttagcacgcgcacgtcgtccggcatcctttatgaagttgatgcgcgt ctgcgtccatctggcgctgcggggatgctggtcactactacggaatcgttcgccgattaccagcaaaaacgaagcctggacgtgggaacatcaggcgctggcccgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatattctgatgacgcctcgcgacggcgcaac gctgcaaaccgacgtgcgagaaatgcgcgagaaaatgcgtgcccatcttggcaacaagcataaagaccgcttcgatctgaaagccgatgaaggcggtatcaccgacatcgagtttatcgcccaatatctggtgctgcgctttgcccatgacaagccgaaactgacgcgctggtcggataatgtgcgcattctcga agggctggcgcaaaacggcatcatggaggcaggaagcgcaggcattgacgctggcgtacaccacattgcgtgatgagctgcaccacctggcgctgcaagagttgccgggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttattaaaaccagctgggacaagtggctggtggaaccgtgcgccccggc gtaa 254 gcgcaaagcgagtgctcacttacgtgatctgttgacacaatctgaagcgaccataacttgccgtttcagcgaatacggcggtgtggagcgcacaatcagccctggcgaagctggtgctcaccgagtggctagtgacgcagggctggcgaaccttccttgatgaaaaagcgcaggccaaattcgccgactcctttaa acgctttgctgacatccatctgtcacgcagcgccgccgagctgaaaaaagccttgcccaaccgctgggcgacagctatcgcgaccagttgccgcgcctggcgcgtgatatcgactgcgcgttactgctggccgggcattacgatcgcgcgcgcgccgtggaatggctggaaaactggcaggggctt cagcacgccattgaaacgcgccagagtcgaaatcgaacatttccgtaataccgcgattacccaggagccgttctggttgcacagcggaaaacgttaacgaaaggatatttcgcatgtttaacgatctgattggcgatgatgaaacggattcgccggaagatgcgctttctgagagctggcgcgaattgtggcaggatgc gttgcaggaggaggattccacgcccgtgctggcgcatctctcagaggacgatcgccgccgcgtggtggcgctgattgccgattttcgcaaagagttggataaacgcaccattggcccgcgagggcggcaggtactcgatcacttaatgccgcatctgctcagcgatgtatgctcgcgcgacgatgcgccagtaccg ctgtcacgcctgacgccgctgctcaccggaattattacccgcaccacttaccttgagctgctaagtgaatttcccggcgcactgaaacacctcatttccctgtgtgccgcgtcgccgatggttgccagtcagctggcgcgctacccgaatcctgcttgaattgctcgacccgaatacgctctatcaaccgacggcga tgaatgcctatcgcgatgagctgcgccaatacctgctgcgcgtgccggaagatgatgaagagcaacagcttgaggcgctgcggcagtttaagcaggcgcagttgctgcgcgtggcggcggcggatattgccggtacgttgccagtaatgaaagtgagcgatcacttaacctggctggcggaagcgattattgatg cggtggtgcagcaagcctggggggcagatggtggcgcgttatggccagccaacgcatctgcacgatcgcgcgaagggcgcggttttgcggtggtcggttatggcaagctgggcggctgggagctgggttacagctccgatctggatctggtattcctgcacgactgcccgatggatgtgatgaccgatggcgagcgtgaaat cgatggtcgccagttctatttgcgtctcgcgcagcgcgtgatgcacctgtttagcacgcgcgcacgtcgtccggcatcctttatgaagttgatgcgcgtctgcgtccatctggcgctgcggggatgctggtcactactacggaatcgttcgccgattaccagcaaaacgaagcctggacgtgggaacatca ggcgctggcccgtgcgcgcgtggtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatattctgatgacgcctcgcgacggcgcaacgctgcaaaccgacgtgcgagaaatgcgcgagaaaatgcgtgcccatcttggcaacaagcataaagaccgcttcgatctgaaag ccgatgaaggcggtatcaccgacatcgagtttatcgcccaatatctggtgctgcgctttgcccatgacaagccgaaactgacgcgctggtcggataatgtgcgcattctcgaagggctggcgcaaaacggcatcatggaggagcaggaagcgcaggcattgacgctggcgtacaccacattgcgtgatgagctgcaccacctggc gctgcaagagttgccgggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttattaaaaccagctgggacaagtggctggtggaaccgtgcgccccggcgtaagtgtggtatcatcgcgcgcaaattttgtatctctcaggagacaggaatgaaagtgacgctgccagagtttaagcaagccggtgta atggtggtgggtgatgtgatgctggatcgttactggtatggcccaaccagccgtatctctccggaagcgccagtcccggttgttaaagtcgataccattgaagagcgtcctggcggcgcggcaaacgtggcgatgaatatcgcctcactgggcgccacggcgcgtctggttggcctgactggcattgacgatgc ggcgcgcgcgctgagcaaagcgctggccgatgttaacgttaaatgtgacttcgtttctgttccgacgcatcccaccatcactaagctgcgcgtgctgtcgcgtaaccagcagctgattcgcctggactttgaagagggttttgaaggagtcgatccgcaaccgatgcatgaacgcatcagccaggcgcttgg taatattggcgcgctggtgctgtcggatt 255 atgaccctgaatatgatgatggatgccggccgtcctgtaataataaccggacaattcggactgattaaaaaagcgcccttgtggcgcttttttttattcccgcctccatttaaaataaaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgttttaacacgcgtttttttaacct tttattgaaagtcggtgcttctttgagcgaacgatcaaatttaagtggattcccatcaaaaaaatattctcaacctaaaaaagttgtgtaatacttgtaacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgcaactcacttcacaccccgaagggggaagttgcctgaccctacgatt cccgctatttcattcactgaccgggaggttcaaaatga 256 accggatacgagagaaaagtgtctacatcggttcggttgatattgaccggcgcatccgccagcccgcccagtttctggtggatctgtttggcgattttgcgggtctgccggtgtcggtgccgaaaaaaataccaatatttgccataacacacgctcctgttgaaaaagagatcccgccgggaaatgcggtgaacgtg tctgatattgcgaagagtgtgccagttttggtcgcgggcaaaacctgcaccagtttggttattaatgcaccagtctggcgctttttttcgccgagtttctcctcgctaatgcccgccaggcgcggctttggcgctgatagcgcgctgaataccgatctggatcaaggttttgtcgggttatcagccaaaaaggtgcactct ttgcatggttatacgtgcctgacatgttgtccgggcgacaaacggcctggtggcacaaattgtcagaactacgacacgactaactgaccgcaggagtgtgcgatgaccctgaatatgatgatggatgccggccgtcctgtaataataaccggacaattcggactgattaaaaaagcgcccttgtggcgcttttttttattcccg cctccatttaaaataaaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgttttaacacgcgttttttaaccttttattgaaagtcggtgcttctttgagcgaacgatcaaatttaagtggattcccatcaaaaaaatttctcaacctaaaaaagtttgtgtaatacttg taacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgcaactcacttcacaccccgaagggggaagttgcctgaccctacgattcccgctatttcattcactgaccggaggttcaaaatgacccagcgaaccgagtcgggtaataccgtctggcgcttcgatttgtcccagcagttcactgcga tgcagcgcataagcgtggtactcagccgggcgaccgaggtcgatcagacgctccagcaagtgctgtgcgtattgcacaatgacgcctttttgcagcacggcatgatctgtctgtacgacagccagcaggcgattttgaatattgaagcgttgcaggaagccgatcagcagttaatccccggcagctcgcaaatccgct atcgtccgggcgaagggctggtcgggacggtgctttcgcagggccaatcattagtgctggcgcgcgttgctgacgatcagcgctttcttgaccggctcgggttgtatgattacaacctgccgtttatcgccgtgccgctgatagggccagatgcgcagactttcggtgtgctgacggcacaacccatggcgcgttac gaagagcgattacccgcctgcacccgctttctggaaacggtc 257 tttcgctgaaggtgtgaccatgggccatcaggtgctggtgcagctggaaagtgttgccatcactatcgtgtggtctggcgtggtggcctttattggttacaaactggcggacatgacggtaggcctgcgcgtaccggaagaacaagaacgtgaagggctggatgtaaacagccacggcgaaaacgcctataacgcc tga 258 tttcctttctgactctgcccgtccgggcgcactaacggcctgaaatactccctcttttcattcctggcacaacgattgcaatgtctgttgcgtgttagctgcggccattatcgaattcgactggagggggatctatgaagctggttaccgtggtgattaagccattcaaacttgaagacgtgcgtgaagcgctttcttctatt ggtattcaagggttgaccgtaactgaagtgaaaggctttggccgtcagaagggtcacgctgagctgtaccgcggtgcggaatatagcgttaatttcctgccgaaagtgaaaattgatgtggcgatcgctgacgatcaactcgatgaagtaatcgatgtgatcagcaaagcggcctacaccggaaaaattggcgacgg caaaattttcgttgctgagctgcaacgcgtgagctgcaacgcgtcattcgtattcgtaccggcgaagccgacgaagcggcactgtaatacaagacacacagtgatggggatcggtttcgctgaaggtgtgaccatgggccatcaggtgctggtgcagctggaaagtgttgccatcactatcgtgtggtctggcgtggtggcctttattgg ttacaaactggcggacatgacggtaggcctgcgcgtaccggaagaacaagaacgtgaagggctggatgtaaacagccacggcgaaaacgcctataacgcctgattgcgttgagttatctcctgagcataaaaaagcctccattcggaggcttttctttttttaagtttaaagcgcggttagttgcgattgcgcatgacgcctt cctgcacgctggacgcgaccagcacaccctcttgcgtatagaactcgccgcgcacaaaaccgcgagcgctggaggctgacgtgctttccacactgtagagcagccattcgttcatattaaacgggcgatggaaccacatggagtggtcaatggtggcaacctgcataccgcgctcaaggaagcccacgccgtgcggctgaagt gcaaccggcaggaagttaaagtctgaggcatatccaagcagatattgatgtacgcgaaaatcgtccggcaccgtgccgtttgcgcggatccatacctggcgggtgggatcggcaacgtggcctttcagcggtttatgaaactcaaccgggcggatctccagtggtttatcactaagaaacttctctttggcctgcgg 259 atgacctttaatatgatgcctggggtcactggagcgctttatcggcatcctgaccgaagaatttgccggtttcttcccgacctggctggcccctgttcaggttgtggtgatgaatatcactgattctcaagctgaatatgtcaacgaattgacccgtaaattgcaaaatgcgggcattcgtgtaaaagcggacttgagaaacgaga agattggctttaaaatccgcgagcacactttacgtcgtgtcccttatatgttggtctgtggtgataaagaggtggaagcaggcaaagtggccgttcgcacccgccgcggtaaagacctgggcagcctggacgtaagtgaagtgattgagaagctgcaacaagagattcgcagccgcagtcttcaacaactggaggaataagg tattaaaggcggaaaacgagttcaaacggcacgtccgaatcgtatcaatggcgagattcgcgcccaggaagttcgcttaactggtctggaaggtgagcagctgggtattgcaatagaactaactacccgccctgaaggcggtacctgcctgaccctgcgattcccgttatttcattcactgaccggaggcccacgatga 260 ggtacgacaaaaacgtctccagcgacgtgcggttaatattgactggcgcatccgccacatcccccagttttgctggatcagtttggcgattttgcgggtttttcccgtgtcactgccaaaaaaataccaatgttagccatgtcgcgctcctgttgagaaagaataaggccgcctgcaaacggcggatatccctt ctcctgttgcgaaggctgtgccaggtttttttaaggccttctgtgcactgaaatgggtgaaaaaatgactctttttgtgcaggcaccgtcctctccgctatccagacctgctttgaaggcctctgaggccaaatcagggccaaaacacgaatcaggatcaatgttcggcgcgttacctgttcgaaaggtgcactct ttgcatggttaatcacacccaatcagggctgcggatgtcgggcgtttcacaacacaaaatgttgtaaatgcgacagccgggcctgaaaccaggagcgtgtgatgacctttaatatgatgcctggggtcactggagcgctttatcggcatcctgaccgaagaatttgccggtttcttcccgacctggctggcccctgttcagg ttgtggtgatgaatatcactgattctcaagctgaatatgtcaacgaattgacccgtaaattgcaaaatgcgggcattcgtgtaaaagcggacttgagaaacgagaagattggctttaaaatccgcgagcacactttacgtcgtgtcccttatatgttggtctgtggtgataaagaggtggaagcaggcaaagtggccg ttcgcacccgccgcggtaaagacctgggcagcctggacgtaagtgaagtgattgagaagctgcaacaagagattcgcagccgcagtcttcaacaactggaggaataaggtattaaaggcggaaaacgagttcaaacggcacgtccgaatcgtatcaatggcgagattcgcgcccaggaagttcgcttaactggtctggaagg tgagcagctgggtattgcaatagaactaactacccgccctgaaggcggtacctgcctgaccctgcgattcccgttatttcattcactgaccggaggcccacgatgacccagcgacccgagtcgggcaccaccgtctggcgttttgatctctcacagcaatttaccgccatgcagcgcatcagcgtggtgttgagtcgcgca accgagataagccagacgctgcaggaggtgctgtgtgttctgcataatgacgcatttatgcaacacggcatgctgtgtctgtatgacaaccagcaggaaattctgagtattgaagccttgcaggaggcagaccaacatctgatccccggcagctcgcaaattcgctatcgccctggcgaagggctggtaggagccgtactgtccca gggacaatctcttgtgctgccgcgtgtcgccgacgatcaacgctttctcgacaggcttggcatctatgattacaacctgccgtttatcgccgtccccttaatggggccaggcgcgcagacgattggcgtgctcgccgcgcagccgatggcgcgtctggaggagcggcttccttcctgtacgcgctttctgga aaccgtc 261 ttgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgaacggtagcacagagagcttgctctcgggtgacgagtggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataccgcataacgtcgcaagaccaaagagggggaccttcgggcctctt gccatcagatgtgcccagatgggattagctagtaggtggggtaacggctcacctaggcgacgatccctagctggtctgaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtgtgaagaaggccttcgggttgta aagcactttcagcggggaggaagggagtaaggttaataaccttattcattgacgttacccgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggtctgtcaagtcggatgtgaaatccccgggctcaacctgggaactgcatccgaa actggcaggcttgagtctcgtagagggaggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggcctcctggacgaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtctatttggaggttgtgcccttgaggcgt ggcttccggagctaacgcgttaaatagaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctggtcttgacatccacagaactttccagagatggattggtgccttcgggaactgtgagacaggtgctgcatggctg tcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatcctttgttgccagcggtccggccgggaactcaaaggagactgccagtgataaactggaggaaggtggggatgacgtcaagtcatcatggcccttacgaccagggctacacacgtgctacaatggcgcatacaaagagaagcgacctc gcgagagtaagcggacctcataaagtgcgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtggatcagaatgccacggtgaatacgttcccggggccttgtacacaccgcccgtcacaccatgggagtgggttgcaaaagaagtaggtagcttaaccttcgggagggcgcttaccact ttgtgattcatgactggggtgaagtcgtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 262 ttgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgaacggtagcacagagagcttgctctcgggtgacgagtggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataccgcataacgtcgcaagaccaaagagggggaccttcgggcctctt gccatcagatgtgcccagatgggattagctagtaggtggggtaacggctcacctaggcgacgatccctagctggtctgaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtgtgaagaaggccttcgggttgta aagcactttcagcggggaggaaggnantanggttaataacctgtgttnattgacgttacccgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggtctgtcaagtcggatgtgaaatccccgggctcaacctgggaactgcatccga aactggcaggcttgagtctcgtagagggaggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggcctcctggacgaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgccgtaaacgatgtctatttggaggttgtgcccttgaggcg tggcttccggagctaacgcgttaaatagaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctggtcttgacatccacagaacttagcagagatgctttggtgccttcgggaactgtgagacaggtgctgcatggct gtcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatcctttgttgccagcggttaggccgggaactcaaaggagactgccagtgataaactggaggaaggtggggatgacgtcaagtcatcatggcccttacgaccagggctacacacgtgctacaatggcgcatacaaagagaagcgacct cgcgagagtaagcggacctcataaagtgcgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtggatcagaatgccacggtgaatacgttcccggggccttgtacacaccgccgtcacaccatgggagtgggttgcaaaagaagtaggtagcttaaccttcgggagggcgcttacc actttgtgattcatgactggggtgaagtcgtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 263 atgaccatgcgtcaatgcgccatttacggcaaaggtgggatcggcaaatcgaccaccacacagaacctggtcgccgcgctggcggagatgggtaaaaaagtcatgattgtcggctgtgacccgaaagccgattccacgcgtttgatcctgcatgcgaaagcgcagaacaccattatggagatggctgctgaagtcggct ccgtggaagacctggagttagaagacgtgctgcaaatcggttacggcggcgtgcgctgcgcagagtccggcggcccggagccaggcgtgggctgtgccggtcgcggggtgatcaccgcgattaacttcctcgaagaagaaggcgcttacgtgccggatctcgattttgttttctacgacgtgctgggcgacgt ggtatgcggtggtttcgccatgccgattcgtgaaaacaaagcgcaggagatctacatcgtttgctctctggcgaaatgatggcgatgtacgccgccaacaacatctccaaaggcatcgtgaaatacgccaaatccggtaaagtgcgcctcggcgggctgatttgtaactcgcgccagaccgaccgtgaagatgaactga tcattgcgctggcagaaaaactcggcacgcagatgatccactttgttccccgcgacaacattgtgcagcgtgcggaaatccgccgtatgacggttatcgaatatgacccgacctgcaatcaggcgaacgaatatcgcagccttgccagcaaaatcgtcaacaacaccaaaatggtggtgcccaccccctgcaccat ggatgaactggaagaactgctgatggagttcggcattatggatgtggaagacaccagcatcattggtaaaaccgccgccgaagaaaacgccgtctga 264 atgagcaatgcaaccaggcgaacgcaacctggagataatcgagcaggtgctcgaggttttcccggagaagacgcgcaaagaacgcagaaaacacatgatggtgacggacccggagcaggaaagcgtcggtaagtgcatcatctctaaccgcaaatcgcagccaggcgtgatgaccgtgcgcggctgctcgtatgccggtt cgaaaggggtggtatttggggccaatcaaggatatggcgcatatctcgcatggcccaatcggctgcggccaatactcccgcgccgggcggcggaactactacaccggcgtcagcggcgtggacagcttcggcacgctcaacttcacctccgattttcaggagcgcgacatcgtgtttggcggcgataaaaagctcgccaaactga ttgaagagctggaagagctgttcccgctgaccaaaggcatttcgattcagtcggaatgcccggtcggcctgattggcgatgacattgaggccgtcgcgaacgccagccgcaaagccatcaacaaaccggttattccggtgcgttgcgaaggctttcgcggcgtgtcgcaatccctcggtcaccatattgccaacgatg tgatccgcgactgggtgctggataaccgcgaaggcaaaccgttcgaatccaccccttacgatgtggcgatcatcggcgattacaaacatcggcggcgatgcctgggcttcgcgcattttgctcgaagatgggcttgcgggtggtggcacagtggtctggcgacggtacgctggtggagatggaaaacacgccg ttcgtcaaactgaacctggtgcattgttaccgtgcattgttaccgctcaatgaactacatctcgcgccatatggaggagaagcacggtattccgtggatggaatacaacttctttggtccgacgaaaatcgcggaatcgctgcgcaaaatcgccgaccagtttgacgacaccattcgcgccaacgccgaagcggtgatcgccagataccaggcgcaa aacgacgccattatcgccaaatatcgcccgcgtctggaggggcgcaaagtgctgctttatatgggcgggctgcgtccgcgccatgtgattggcgcctatgaagacctgggaatggagatcatcgctgccggttatgagttcggtcataacgatgattacgaccgcaccttgccggatctgaaagagggcacgctgctgt ttgatgatgccagcagttatgagctggaggcgttcgtcaacgcgctgaaaccggatctcatcggttccggcatcaaagaagtacatctttcagaaaatgggcgtgccgtttcgccagatgcactcctgggattactccggcccgtaccacggctatgacggcttcgccatcttcgcccgcgatatggatatgacgctcaaca accccgcgtggggccagttgaccgcgccgtggctgaaatccgcctga 265 atgagccagactgctgagaaaatacagaattgccatcccctgtttgaacaggatgcttaccagacgctgttgccggtaaacgggcactcgaagaggcgcactcgccggagcgggtgcaggaagtgtttcaatggaccactaccccggaatatgaagcgctgaactttaaacgcgaagcgctgactatcgacccggcaaaagcc tgccagccgctgggcgcggtgctctgttcgctggggtttgccaataccctaccgtatgtgcacggttcacagggttgcgtggcctatttccgcacgtactttaaccgccactttaaagaaccggtggcctgcgtgtcggattcaatgacggaagacgcggcggtgttcggcgggaataaccctcaacaccgg cttacaaaacgccagcgcgctgtataaaccggagattatcgccgtctctaccacctgtatggcggaagtgatcggtgatgatttgcaggcctttcgccaacgccaaaaaagatggttttctcgatgccgccatccccgtgccctacgcgcacacccccagttttatcggcagccatatcaccggctgggataacatgt ttgaaggttttgcccggaccttttacggcagaccatgaagctcagccggcaaactttcacgcatcaacctggtgaccgggtttgaaacctatctcggcaatttccgcgtgctgaaacgcatgatggaacaaatggaggtgccggcgagtgtgctctccgatccgtcggaagtgctggatactccgccaacgggcattaccagatgt acgcgggcgggacgacgcagcaagagatgcgcgaggcgccggatgctatcgacaccctgttgctgcagccctggcaactggtgaaaagcaaaaaagtggtgcaggagatgtggaatcagcccgccaccgaggttctgttcccgttgggctggcaggaacagacgaactgttgatggcgattagccagttaaccggcaagg ccattcccgattcactggcgctggagcgcgggcggctggtcgatatgatgctcgattcccacacctggttgcacggtaaaaaattcggcctgtttggcgatccggatttgtcatgggattgacccgtttcctgctggagctgggctgcgaaccgaccgttatcctctgccacaacggtaacaaacgctggcagaaagcaat gaagaaaatgcttgacgcctcgccgtacggccaggagagcgaagtgtttatcaactgcgatttgtggcatttccgctcgctgatgtttacccgccagccggattttatgattggcaactcgtacggcaagttcattcagcgcgacaccttagccaaaggcgagcagtttgaagttccgctgatccgcctcggttttcccctgtt cgaccgccaccatctgcaccgccagaccacctggggctacgaggccgccatgagcattctcactacccttgtgaatgcggtactggagaaagtggacaaagagaccatcaagctcggcaaaaccgactacagcttcgatcttatccgttaa 266 atgaccctgaatatgatgatggatgccggcgcgcccgaggcaatcgccggtgcgctttcgcgacaccatcctgggctgttttttaccatcgttgaagaagcgcccgtcgccatttcgctgactgatgccgacgcacgcattgtctatgccaacccggctttctgccgccagaccggctatgaactagaagcgttgttgcagca aaatccccgcctgcttgcaagtcgccaaaccccacgggaaatctatcaggatatgtggcacaccttgttacaacgccgaccgtggcgcgggcaattgattaaccgccaccgcgacggcagcctgtatctggtcgagatcgatatcaccccggtgattaacccgtttggcgaactggaacactacctggcaatgcagcgcgata tcagcgccagttatgcgctggagcagcggttgcgcaatcacatgacgctgaccgaagcggtgctgaataacattccggcggcggtggttgtagtggatgaacgcgatcatgtggttatggataaccttgcctacaaaacgttctgtgccgactgcggcggaaaagagctcctgagcgaactcaatttttcagcccgaaaagc ggagctggcaaacggccaggtcttaccggtggtgctgcgcggtgaggtgcgctggttgtcggtgacctgctgggcgctgccgggcgtcagcgaagaagccagtcgctactttattgataacaggctgacgcgcacgctggtggtgatcaccgacgacacccaacaacgccagcagcaggaacagggccgacttgaccg ccttaaacagcagatgaccaacggcaaactactggcagcgatccgcgaagcgcttgacgccgcgctgatccagcttaactgccccatcaatatgctggcggcggcgcgacgtttaaacggcagtgataacaacaatgtggcgctcgacgccgcgtggcgcgaaggtgaagaggcgatggcgcggctgaaacgttg ccgcccgtcgctggaactggaaagtgcggccgtctggccgctgcaacccttttttgacgatctgcgcgcgcttttcacacccgctacgagcagggggaaaaatttgcaggtcacgctggattcccatcatctggtgggatttggtcagcgtacgcaactgttagcctgcctgagtctgtggctcgatcgcac gctggatattgccgccgggctgggtgatttcaccgcgcaaacgcagatttacgcccgcgaagaagagggctggctctctttgtatatcactgacaatgtgccgctgatcccgctgcgccacacccactcgccggatgcgcttaacgctccgggaaaaggcatggagctgcgcctgatccagacgctggtggcacaccaccacggc gcaatagaactcacttcacaccccgaagggggaagttgcctgaccctacgattcccgctatttcattcactgaccgggaggttcaaaatga 267 atgacccagcgaaccgagtcgggtaataccgtctggcgcttcgatttgtcccagcagttcactgcgatgcagcgcataagcgtggtactcagccgggcgaccgaggtcgatcagacgctccagcaagtgctgtgcgtattgcacaatgacgcctttttgcagcacggcatgatctgtctgtacgacagcca gcaggcgattttgaatattgaagcgttgcaggaagccgatcagcagttaatccccggcagctcgcaaatccgctatcgtccgggcgaagggctggtcgggacggtgctttcgcaggccaatcattagtgctggcgcgcgttgctgacgatcagcgctttcttgaccggctcgggttgtatgattacaacctgccgtttat cgccgtgccgctgatagggccagatgcgcagactttcggtgtgctgacggcacaacccatggcgcgttacgaagagcgattacccgcctgcacccgctttctggaaacggtcgctaacctggtcgcgcaaaccgtgcgttgatggcaccaccggcagtgcgcccttccccgcgcgccgccataacacagg ccgccagcccgaaatcctgcacggcctcacgcgcatttggttttgaaaatatggtcggtaacagtccggcgatgcgccagaccatggagattatccgtcaggtttcgcgctgggacaccaccgttctggtacgcggcgagtggcaccggcaaggagctgattgccaacgccatccaccaccattcgccgcgtgccggtgcgcca tttgtgaaattcaactgtgcggcgctgccggacacactgctggaaagcgaattgttcggtcacgagaaaggggcatttaccggcgcggtacgccagcgtaaaggccgttttgagctggccgatggcggcacgctgtttcttgacgagatcggcgagtagcgcctcgtttcaggctaagctgctgcgcatt ttgcaggaaggcgaaatggaacgcgtcggcggcgacgagacattgcaagtgaatgtgcgcattattgccgcgacgaaccgcaatcttgaagatgaagtccggctggggcactttcgcgaagatctctattatcgcctgaatgtgatgcccatcgccctgccgccactacgcgaacgccaggaggacattgccgagctggcg cactttctggtgcgtaaaatcgcccataaccagagccgtacgctgcgcattagcgagggcgctatccgcctgctgatgagctacaactggcccggtaatgtgcgcgaactggaaaactgccttgagcgctcagcggtgatgtcggagaacggtctgatcgatcgggatgtgattttgtttaatcatcgcgaccag ccagccaaaccgccagttatcagcgtctcgcatgatgataactggctcgataacaaccttgacgagcgccagcggctgattgcggcgctggaaaaagcgggatgggtacaagccaaagccgcgcgcttgctggggatgacgccgcgccaggtcgcctatcgtattcagacgatggatataaccctgccaaggctataa 268 atgccgcaccacgcaggattgtcgcagcactggcaaacggtattttctcgtctgccggaatcgctcaccgcgcagccattgagcgcgcaggcgcagtcagtgctcacttttagtgattttgttcaggacagcatcatcgcgcatcctgagtggctggcagagcttgaaagcgcgccgccgcctgcgaacgaatgg caacactatgcgcaatggctgcaagcggcgctggatggcgtcaccgatgaagcctcgctgatgcgcgcgctgcggctgtttcgccgtcgcatcatggtgcgcatcgcctggagccaggcgttacagttggtggcggaagaagaagatatcctgcaacagcttagcgtgctggcggaaaccctgatcgtcgccgc gcgcgactggctttatgaggcctgctgccgtgagcctgctgccgtgagtggggaacgccgagcaatccacaaggcgtggcgcagccgatgctggtactcggcatgggcaaactgggtggcggcgaactcaatttctcatccgatatcgatttgattttcgcctggccggaaaatggcgcaacgcgcggtggacgccgtgagctggataacgcg caatttttcactcgccttggtcaacggctgattaaagtcctcgaccagccaacgcaggatggctttgtctaccgcgcttgcgcccgttggcgacagcggcccgctggtgctgagctttgccgcgctggaagattactaccaggagcaggggcgcgattgggaacgctacgcgatggtgaaagcgcg cattatgggcgataacgacggcgaccatgcgcgggagttgcgcgggagttgcgcgcaatgctgcgccgtttgttttccgccgttatcgacttcagcgtgattcagtccctgcgtaacatgaaaggcatgattgcccgcgaagtgcgcgccgtggcctgaaggacaacattaagctcggcgcgggcgggatccgcga aatagaatttatcgtccaggttttccagctgattcgcggcggtcgcgagcctgcactgcaatcgcgttcactgttgccgacgcttgctgccatagatcaactgcatctgctgccggatggcgacgcaacccggctgcgcgaggcgtatttgtggctgcgacggctggagaacctgctgcaaagcatcaatgacgaacagacacag . atgcgttgcaggaggaggattccacgcccgtgctggcgcatctctcagaggacgatcgccgccgcgtggtggcgctgattgccgattttcgcaaagagttggataaacgcaccattggcccgcgagggcggcaggtactcgatcacttaatgccgcatctgctcagcgatgtatgctcgcgcgacgatgcgccag taccgctgtcacgcctgacgccgctgctcaccggaattattacccgcaccacttaccttgagctgctaagtgaatttcccggcgcactgaaacacctcatttccctgtgtgccgcgcgccgccgatggttgccagtcagctggcgcgctacccgatcctgcttgatgaattgctcgacccgaatacgctctatcaaccgac ggcgatgaatgcctatcgcgatgagctgcgccaatacctgctgcgcgtgccggaagatgatgaagagcaacagcttgaggcgctgcggcagtttaagcaggcgcagttgctgcgcgtggcggcggcggatattgccggtacgttgccagtaatgaaagtgagcgatcacttaacctggctggcggaagcgattatt gatgcggtggtgcagcaagcctggggggcagatggtggcgcgttatggccagccaacgcatctgcacgatcgcgaagggcgcggttttgcggtggtcggttatggcaagctgggcggctgggagctgggttacagctccgatctggatctggtattcctgcacgactgcccgatggatgtgatgaccgatggcgagcgt gaaatcgatggtcgccagttctatttgcgtctcgcgcagcgcgtgatgcacctgtttagcacgcgcacgtcgtccggcatcctttatgaagttgatgcgcgtctgcgtccatctggcgctgcggggatgctggtcactactacggaatcgttcgccgattaccagcaaaacgaagcctggacgtggga acatcaggcgctggccgtgcgcgcgtggtgtgtacggcgatccgcaactgaccgccgaatttgacgccattcgccgcgatattctgatgacgcctcgcgacggcgcaacgctgcaaaccgacgtgcgagaaatgcgcgagaaaatgcgtgcccatcttggcaacaagcataaagaccgcttcgatct gaaagccgatgaaggcggtatcaccgacatcgagtttatcgcccaatatctggtgctgcgctttgcccatgacaagccgaaactgacgcgctggtcggataatgtgcgcattctcgaagggctggcgcaaaacggcatcatggaggagcaggaagcgcaggcattgacgctggcgtacaccacattgcgtgatgagctgcaccacc tggcgctgcaagagttgccgggacatgtggcgctctcctgttttgtcgccgagcgtgcgcttattaaaaccagctgggacaagtggctggtggaaccgtgcgccccggcgtaa 269 attgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgaacggtagcacagagagcttgctctcgggtgacgagtggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataccgcataacgtcgcaagaccaaagagggggaccttcgggcctctt gccatcagatgtgcccagatgggattagctagtaggtggggtaacggctcacctaggcgacgatccctagctggtctgaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtgtgaagaaggccttcgggttgta aagcactttcagcggggaggaagggagtaaggttaataaccttgctcattgacgttacccgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggtctgtcaagtcggatgtgaaatccccgggctcaacctgggaactgcatccga aactggcaggcttgagtctcgtagagggaggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggcctcctggacgaagactgacgctcaggtgcgaaagnnnnnnnnnnnaacaggattagataccctggtagtccatgccgtaaacgatgtctactagccgttggggcctttgaggctttagtggc gcagctaacgcgataagtagaccgcctggggagtacggtcgcaagactaaaanctcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctggccttgacatagtaagaattttccagagatggattggtgccttcgggaacttacatacaggtgctgcatggctgtcgtca gctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccttgtcattagttgctacatttagttgggcactctaatgagactgccggtgacaaaccggaggaaggtggggatgacgtcaagtcctcatggcccttataggtggggctacacacgtcatacaatggctggtacaaagggttgccaacccgcgagggggag ctaatcccataaaaccagtcgtagtccggatcgcagtctgcaactcgactgcgtgaagtcggaatcgctagtaatcgtggatcagaatgtcacggtgaatacgttcccgggtcttgtacacaccgcccgtcacaccatgggagcgggttctgccagaagtagttagcttaaccgcaaggagggcgattaccacggcaggttt cgtgactggggtgaagtcgtaacaaggtagccgtatcggaaggtgcggctggatcacctccttt 270 atgaccatgcgtcaatgcgccatttacggcaaaggtgggatcggcaaatcgaccaccacacagaacctggtcgccgcgctggcggagatgggtaaaaaagtcatgattgtcggctgtgacccgaaagccgattccacgcgtttgatcctgcatgcgaaagcgcagaacaccattatggagatggctgctgaagtcggct ccgtggaagacctggagttagaagacgtgctgcaaatcggttacggcggcgtgcgctgcgcagagtccggcggcccggagccaggcgtgggctgtgccggtcgcggggtgatcaccgcgattaacttcctcgaagaagaaggcgcttacgtgccggatctcgattttgttttctacgacgtgctgggcgacgt ggtatgcggtggtttcgccatgccgattcgtgaaaacaaagcgcaggagatctacatcgtttgctctctggcgaaatgatggcgatgtacgccgccaacaacatctccaaaggcatcgtgaaatacgccaaatccggtaaagtgcgcctcggcgggctgatttgtaactcgcgccagaccgaccgtgaagatgaactga tcattgcgctggcagaaaaactcggcacgcagatgatccactttgttccccgcgacaacattgtgcagcgtgcggaaatccgccgtatgacggttatcgaatatgacccgacctgcaatcaggcgaacgaatatcgcagccttgccagcaaaatcgtcaacaacaccaaaatggtggtgcccaccccctgcaccat ggatgaactggaagaactgctgatggagttcggcattatggatgtggaagacaccagcatcattggtaaaaccgccgccgaagaaaacgccgtctga 271 atggccgaaattctgcgcagtaaaaaaccgctggcggtcagcccgataaaaagcggccagccgctgggggcgatcctcgcaagcctgggtgtcgaacagtgcataccgctggtacacggcgcacagggatgtagcgcgttcgcgaaggtgttctttattcaacattttcacgatccgatcccgctg caatcgacggcgatggatccgacttccaccattatgggcgccgatgaaaacatttttaccgcgctcaatgtgctctgccagcgcaacgccgcgaaagccattgtgctgctcagcaccgggctttcagaagcccagggcagcgacatttcgcgggtggtgcgccagtttcgtgatgattttccccggcataaaggcgtt gcgctgctcaccgtcaacacccgatttctacggctcgctggaaaacggctacagcgccgtgctggaaagcatgattgaacagtgggtacccgcacagcccgccgccagcctgcgcaaccgccgtgtcaacctgctggtcagccatttactgacaccaggcgatatcgaactgttgcgcagttatgttgaagccttcgg cctgcaaccggtgattgtgccggatctgtcgctgtcgctggacgggcatctggcagacggtgatttttcgcctgttacccaagggggaacatcgctgcgcatgattgaacagatggggcaaaacctggccaccttgtgattggcgcctcgctgggccgtgcggcggcgttactggcgcagcgcagccgtggcga ggtgatcgccctgccgcatctgatgacgcttgcagcctgcgacacgtttattcatcgactgaaaaccctccgggcgcgatgtccccgcgtggattgagcgccagcgcggccaagttcaggatgcgatgatcgattgccatatgtggctgcagggtgcggctatcgccatggcagcagaaggcgatcacc tggcggcatggtgcgatttcgcccgcagccagggcatgatccccggcccgattgtcgcaccggtcagccagccggggttgcaaaatctgccggttgaaaccgtggttatcggcgatctggaagatatgcaggatcggctttgcgcgacgcccgccgcgttactggtggccaattctcatgccgccgatctcgccacgca gtttgatttgtcacttatccgcgccgggttcccggtgtatgaccggctgggggaatttcgtcgcctgcgccaggggtacagcggcattcgtgacacgctgtttgagctggcgaatgtgatgcgcgagcgccatcacccgcttgcaacctaccgctcgccgctgcgccagcacgccgacgacaacgtt acgcctggagatctgtatgccgcatgttaa 272 atgaaaaacacaacattaaaaacagcgcttgcttcgctggcgttactgcctggcctggcgatggcggctcccgctgtggcggataaagccgacaacggcttttgatgatttgcaccgcgctggtgctgtttatgaccattccgggcattgcgctgttctacggcggtttgatccgcggtaaaaacgtgctgt cgatgctgacgcaggttgccgtcaccttcgcactggtgtgcattctgtgggtggtgtatggctactcgctggcatttggcgagggcaacagcttcttcgggagttttaactgggcgatgttgaaaaacatcgaactgaaagccgtgatgggcagcatttatcagtatccacgtggcgttccagggttccttcgcct gtatcaccgttggcctgattgtcggtgcactggctgagcgtattcgcttctgcggtgctgatttttgtggtggtatggctgacgctttcttacgtgccgattgcacacatggtgtggggcggcggtctgctggcaacccacggtgcgctggatttcgcaggcggtacggttgttcacatcaacgctgcgattgca ggtctggtgggggcttacctgattggcaaacgcgtgggctttggcaaagaagcattcaaaccgcataacctgccgatggtcttcactggcaccgctatcctgtatgttggctggtttggtttcaacgccggctccgcaagctcggcgaacgaaattgctgcgctggccttcgtgaacactgtcgttgccactgctgccgctat tctggcgtgggtatttggcgaatgggcaatgcgcggcaagccgtctctgctcggtgcctgttctggtgccatcgcgggtctggttggtatcacccccgcctgtggttatgtgggtgtcggcggtgcgctgattgtgggtctgattgccggtctggctgggctgtggggcgttactgcgctgaaacg tatgttgcgtgtcgatgacccgtgtgacgtattcggtgtgcacggcgtgtgcggcatcgtgggctgtatcctgacgggtatcttcgcctctacgtcgctgggtggtgtcggtttcgctgaaggtgtgaccatgggccatcaggtgctggtgcagctggaaagtgttgccatcact atcgtgtggtctggcgtggtggcctttattggttacaaactggcggacatgacggtaggcctgcgcgtaccggaagaacaagaacgtgaagggctggatgtaaacagccacggcgaaaacgcctataacgcctga 273 cgtcctgtaataataaccggacaattcggactgattaaaaaagcgcccttgtggcgcttttttttattcccgcctccatttaaaataaaaatccaatcggatttcactatttaaactggccattatctaagatgaatccgatggaagctcgctgttttaacacgcgttttttaaccttttattgaaagtcggtgcttctttga gcgaacgatcaaatttaagtggattcccatcaaaaaaatattctcaacctaaaaaagttgtgtaatacttgtaacgctacatggagattaactcaatctagagggtattaataatgaatcgtactaaactggtactgggcgc 274 ggacatcatcgcgacaaacaatattaataccggcaaccacaccggcaatttacgagactgcgcaggcatcctttctcccgtcaatttctgtcaaataaagtaaaagaggcagtctacttgaattaccccggctggttgcgtttgttgaaaaaaagtaactgaaaaatccgtagaatagcgccactctgatggttaattaacctattcaattaagaatta tctggatgaatgtgccattaaatgcgcagcataatggtgcgttgtgcgggaaaactgcttttttttgaaagggttggtcagtagcggaaac 275 atgaccctgaatatgatgctcgataacgccgcgccggaggccatcgccggcgcgctgactcaacaacatccggggctgttttttaccatggtggaacaggcctcggtggccatctccctcaccgatgccagcgccaggatcatttacgccaacccggcgttttgccgccagaccggctattcgctggcgcaattgttaaaccagaaccc gcgcctgctggccagcagccagacgccgcgcgagatctatcaggagatgtggcataccctgctccagcgtcagccctggcgcggtcagctgattaatcagcgtcgggacggcggcctgtacctggtggagattgacatcaccccggtgcttagcccgcaaggggaactggagcattatctggcgatgcagcgggatatcagcg tcagctacaccctcgaacagcggctgcgcaaccatatgaccctgatggaggcggtgctgaataatatccccgccgccgtggtagtggtggacgagcaggatcgggtggtgatggacaacctcgcctacaaaaccttctgcgctgactgcggcggccgggagctgctcaccgagctgcaggtctcccctggccggatgacgccc ggcgtggaggcgatcctgccggtggcgctgcgcggggccgcgcgctggctgtcggtaacctgctggccgttgcccggcgtcagtgaagaggccagccgctactttatcgacagcgcgctggcgcggaccctggtggtgatcgccgactgtacccagcagcgtcagcagcaggagcaagggcgccttgacc ggctgaagcagcaaatgaccgccggcaagctgctggcggcgatccgcgagtcgctggacgccgcgctgatccagctgaactgcccgattaatatgctggcggcagcccgtcggctgaacggcgagggaagcgggaatgtggcgctggaggccgcctggcgtgaaggggaagaggcgatggcgcggctccagcgctgtcg cccatcgctggaactcgaaaaccccgccgtctggccgctgcagccctttttcgacgatctgtgcgccctctaccgtacacgcttcgatcccgacgggctgcaggtcgacatggcctcaccgcatctgatcggctttggccagcgcaccccactgctggcgtgcttaagcctgtggctcgatcgcaccctggccctcg ccgccgaactcccctccgtgccgctggcgatgcagctctacgccgaggagaacgacggctggctgtcgctgtatctgactgacaacgtaccgctgctgcaggtgcgctacgctcactcccccgacgcgctgaactcgccgggcaaaggcatggagctgcggctgatccagaccctggtggcgcaccatcgcggggccattgagct ggcttcccgaccgcagggcggcacctgcctgaccctgcgtttcccgctgtttaacaccctgaccgggaggtgaagcatga 276 atgatccctgaatccgacccggacaccaccgtcagacgcttcgacctctctcagcagttcaccgccatgcagcggataagcgtggtgctgagccgggccaccgaggccagcaaaacgctgcaggaggtgctcagcgtattacacaacgatgcctttatgcagcacgggatgatctgcctgtacgacagcgagcaggagat cctcagtatcgaagcgctgcagcaaaccggccagcagcccctccccggcagcacgcagatccgctatcgccccggcgagggactggtggggaccgtgctggcccaggggcagtcgctggtgctgccccgggtcgccgacgatcagcgttttctcgaccgcctgagcctctacgattacgatctgccgtttatcg ccgtaccgttgatggggcccaacgcccggccaataggggtgctggcggcccagccgatggcgcgccaggaagagcggctgccggcctgcacccgttttctcgaaaccgtcgccaacctcgtcgcccagaccatccggctgatgatccttccggcctcacccgccctgtcgagccgccagccgccgaaggtggaacggccgcc ggcctgctcgtcgtcgcgcggcgtgggccttgacaatatggtcggcaagagcccggcgatgcgccagatcgtggaggtgatccgtcaggtttcgcgctgggacaccaccgtgctggtacgcggcgaaagcggcaccgggaaagagctgatcgccaacgccatccatcaccattcgccacgggctggcgccg ccttcgtcaaatttaactgcgcggcgctgccggacaccctgctggaaagcgaactgttcggccatgagaaaggcgcctttaccggggcggtgcgtcagcgtaaaggacgttttgagctggcggatggcggcaccctgttcctcgatgagattggtgaaagcagcgcctcgttccaggccaagctgctgcgtatcc tccaggagggggagatggagcgggtcggcggcgatgagaccctgcgggtgaatgtccgcatcatcgccgccaccaaccgtcacctggaggaggaggtccggctgggccatttccgcgaggatctctactatcgtctgaacgtgatgcccatcgccctgcccccgctgcgcgagcgtcaggaggacatcgccgagctggcgc acttcctggtgcgcaaaatcggccagcatcaggggcgcacgctgcggatcagcgagggcgcgatccgcctgctgatggagtacagctggccgggtaacgttcgcgaactggagaactgcctcgaacgatcggcggtgatgtcggagagtggcctgatcgatcgcgacgtgatcctcttcactcaccaggatcg tcccgccaaagccctgcctgccagcggggccagcggaagacagctggctggacaacagcctggacgaacgtcagcgactgatcgccgcgctggaaaaagccggctgggtgcaggccaaggcggcacggctgctggggatgacgccgcgccaggtcgcttatcggatccagatcatggatatcaccctgccgcgtctgtag 277 attgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgagcggcagcgggaagtagcttgctactttgccggcgagcggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataccgcatgacctcgaaagagcaaagtgggggatcttcggacct cacgccatcggatgtgcccagatgggattagctagtaggtgaggtaatggctcacctaggcgacgatccctagctggtctgaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtgtgaagaaggccttagggttg taaagcactttcagcgaggaggaaggcatcatacttaatacgtgtggtgattgacgttactcgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggtttgttaagtcagatgtgaaatccccgagcttaacttgggaactgcattt gaaactggcaagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgctgtaaacgatgtcgacttggaggttgtgcccttgaggc gtggcttccggagctaacgcgttaagtcgaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctactcttgacatccagagaatttgccagagatggcgaagtgccttcgggaactctgagacaggtgctgcatgg ctgtcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatccttgttgccagcacgtaatggtgggaactcaaaggagactgccggtgataaaccggaggaaggtggggatgacgtcaagtcatcatggcccttacgagtagggctacacacgtgctacaatggcatatacaaagagaagcgaact cgcgagagcaagcggacctcataaagtatgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtagatcagaatgctacggtgaatacgttcccgggccttgtacacaccgcccgtcacaccatgggagtgggttgcaaaagaagtaggtagcttaaccttcgggagggcgcttaccacttt gtgattcatgactggggtgaagtcgtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 278 attgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgagcggcatcgggaagtagcttgctactttgccggcgagcggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataccgcatgacctcgaaaagagcaaagtgggggatcttcggacctc . aagcactttcagcgaggaggaaggcatcatacttaatacgtgtggtgattgacgttactcgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggttgttaagtcagatgtgaaatccccgagcttaacttgggaactgcatttga aactggcaagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgctgtaaacgatgtcgacttggaggttgtgcccttgaggcg tggcttccggagctaacgcgttaagtcgaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctactcttgacatccagagaatttgccagagatggcgaagtgccttcgggaactctgagacaggtgctgcatggct gtcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatcctttgttgccagcacgtgatggtgggaactcaaaggagactgccggtgataaaccggaggaaggtggggatgacgtcaagtcatcatggcccttacgagtagggctacacacgtgctacaatggcatatacaaagagaagcgaact cgcgagagcaagcggacctcataaagtatgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtagatcagaatgctacggtgaatacgttcccgggccttgtacacaccgcccgtcacaccatgggagtgggttgcaaaagaagtaggtagcttaaccttcgggagggcgcttaccacttt gtgattcatgactggggtgaagtcgtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 279 attgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgagcggcagcgggaagtagcttgctactttgccggcgagcggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataccgcatgacctcgaaagagcaaagtgggggatcttcggacct cacgccatcggatgtgcccagatgggattagctagtaggtgaggtaatggctcacctaggcgacgatccctagctggtctgaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtgtgaagaaggccttagggttg taaagcactttcagcgaggaggaaggcancatacttaatacgtgtggtgattgacgttactcgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggtttgttaagtcagatgtgaaatccccgagcttaacttgggaactgcattt gaaactggcaagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgctgtaaacgatgtcgacttggaggttgtgcccttgaggc gtggcttccggagctaacgcgttaagtcgaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctactcttgacatccagagaatttgccagagatggcgaagtgccttcgggaactctgagacaggtgctgcatgg ctgtcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatccttgttgccagcacgtgatggtgggaactcaaaggagactgccggtgataaaccggaggaaggtggggatgacgtcaagtcatcatggcccttacgagtagggctacacacgtgctacaatggcatatacaaagagaagcga actcgcgagagcaagcggacctcataaagtatgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtagatcagaatgctacggtgaatacgttcccggggccttgtacacaccgcccgtcacaccatgggagtgggttgcaaaagaagtaggtagcttaaccttcgggagggcgcttaccact ttgtgattcatgactggggtgaagtcgtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 280 attgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgagcggcancgggaagtagcttgctactttgccggcgagcggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataatccgcatgacctcgaaaagagcaaagtgggggatcttcggacctc . aagcactttcagcgaggaggaaggcatcacacttaatacgtgtgtgattgacgttactcgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggttgttaagtcagatgtgaaatccccgagcttaacttgggaactgcattt gaaactggcaagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgctgtaaacgatgtcgacttggaggttgtgcccttgaggc gtggcttccggagctaacgcgttaagtcgaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctactcttgacatccagagaatttgccagagatggcgaagtgccttcgggaactctgagacaggtgctgcatgg ctgtcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatccttgttgccagcacgtgatggtgggaactcaaaggagactgccggtgataaaccggaggaaggtggggatgacgtcaagtcatcatggcccttacgagtagggctacacacgtgctacaatggcatatacaaagagaagcga actcgcgagagcaagcggacctcataaagtatgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtagatcagaatgctacggtgaatacgttcccggggccttgtacacaccgcccgtcacaccatgggagtgggttgcaaaagaagtaggtagcttaaccttcgggagggcgcttaccact ttgtgattcatgactggggtgaagtcgtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 281 attgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgagcggcagcgggaagtagcttgctactttgccggcgagcggcggacgggtgagtaatgtctgggaaactgcctgatggagggggataactactggaaacggtagctaataccgcatgacctcgaaagagcaaagtgggggatcttcggacct cacgccatcggatgtgcccagatgggattagctagtaggtgaggtaatggctcacctaggcgacgatccctagctggtctgaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtgtgaagaaggccttagggttg taaagcactttcagcgaggaggaaggcatcacacttaatacgtgtgttgattgacgttactcgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcaggcggtttgttaagtcagatgtgaaatccccgagcttaacttgggaactgcat ttgaaactggcaagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagataccctggtagtccacgctgtaaacgatgtcgacttggaggttgtgcccttgagg cgtggcttccggagctaacgcgttaagtcgaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctactcttgacatccagagaatttgccagagatggcgaagtgccttcgggaactctgagacaggtgctgcat ggctgtcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatccttgttgccagcacgtgatggtgggaactcaaaggagactgccggtgataaaccggaggaaggtggggatgacgtcaagtcatcatggcccttacgagtagggctacacacgtgctacaatggcatatacaaagagaagc gaactcgcgagagcaagcggacctcataaagtatgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtagatcagaatgctacggtgaatacgttccgggccttgtacacaccgcccgtcacaccatgggagtgggttgcaaaagaagtaggtagcttaaccttcgggagggcgcttacc actttgtgattcatgactggggtgaagtcgtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 282 gtagctaataccgcatgacctcgaaagagcaaagtgggggatcttcggacctcacgccatcggatgtgcccagatgggattagctagtaggtgaggtaatggctcacctaggcgacgatccctagctggtctgaggatgaccagccacactggaactgaccacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcg caagcctgatgcagccatgccgcgtgtgtgaagaaggccttagggttgtaaagcactttcagcgaggaggaaggcatcanacttaatacgtgtgntgattgacgttactcgcagaagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcgttaatcggaattactgggcgtaaagcgcacgcagg cggtttgttaagtcagatgtgaaatccccgagcttaacttgggaactgcatttgaaactggcaagctagagtcttgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattagatccactgg tagtccacgctgtaaacgatgtcgacttggaggttgtgcccttgaggcgtggcttccggagctaacgcgttaagtcgaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctactcttgacatccag agaatttgccagagatggcgaagtgccttcgggaactctgagacaggtgctgcatggctgtcgtcagctcgtgttgtgaaatgttgggttaagtcccgcaacgagcgcaacccttatcctttgttgccagcacgtnatggtgggaactcaaaggagactgccggtgataaac 283 attgaagagtttgatcatggctcagattgaacgctggcggcaggcctaacacatgcaagtcgagcggcagcgggaagtagcttgctactttgccggcgagcggcggacgggtgagtaatgtcctgatggaggggataactactggaacggtagctaataccgcacctcgaaagagcaaagtgggggatcttcggacctcacgccatcggat gtgcccagatgggattagctagtaggtgaggtaatggctcacctaggcgacgatccctagctggtctgagaggatgaccagccacactggaactgagacacggtccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagccatgccgcgtgtgtgaagaaggccttagggttgtaaagcactttcag cgaggaggaaggcatcatacttaatacgtgtggtgattgacgttactcgcagaagaagcaccggctaactccgtgccagcagccgcggtaatacggagggtgcaagcttaatcggaattactgggcgtaaagcgcacgcaggcggttgttaagtcagatgtgaaatccccgagcttaacttgggaactgcatttgaaactggcaagctagagtct tgtagaggggggtagaattccaggtgtagcggtgaaatgcgtagagatctggaggaataccggtggcgaaggcggccccctggacaaagactgacgctcaggtgcgaaagcgtggggagcaaacaggattaataccctggtagtccacgctgtaacgatgtcgacttggaggttgtgccctgaggcgtggcttccggagctaacgcg ttaagtcgaccgcctggggagtacggccgcaaggttaaaactcaaatgaattgacggggcccgcacaagcggtggagcatgtggtttaattcgatgcaacgcgaagaaccttacctactcttgacatccagagaatttgccagagatggcgaagtgccttcgggaactctgagacaggtgctgcatggctgtcgtcagctcgtgtt gtgaaatgttgggttaagtcccgcaacgagcgcaacccttatcctttgttgccagcacgtaatggtgggaactcaaaggagactgccggtgataaaccggaggaaggtggggatgacgtcaagtcatcatggcccttacgagtagggctacacacgtgctacaatggcatatacaaagagaagcgaactcgcgagagcaagcggacctcata aagtatgtcgtagtccggattggagtctgcaactcgactccatgaagtcggaatcgctagtaatcgtagatcagaatgctacggtgaatacgttcccgggccttgtacacaccgcccgtcacaccatgggagtgggttgcaaaagaagtaggtagcttaaccttcgggagggcgcttaccactttgtgattcatgactggggtgaagtc gtaacaaggtaaccgtaggggaacctgcggttggatcacctcctt 284 atgaccatgcgtcaatgcgctatctacggtaaaggcggtatcggtaaatccaccaccacccagaatctcgtcgcggccctcgccgagatgggtaagaaagtgatgatcgtcggctgcgatccgaaagcggattccacccgtctgatcctccacgctaaagcccagaacaccatcatggagatggcggcggaagtgggctc ggtcgaggatctggagctcgaagacgttctgcaaatcggctatggcgatgtccgttgcgccgaatccggcggcccggagccaggcgtcggctgcgccggacgcggggtgatcaccgccatcaacttcctcgaggaagaaggcgcctatgaagaagatttggatttcgtcttctatgacgtcctcggcgacgtggtctg cggcggcttcgctatgccgatccgcgaaaacaaagcccaggagatctacatcgtctgctccggcgagatgatggcgatgtatgccgccaacaatatctccaaagggatcgtgaagtacgccaaatccggcaaggtgcgcctcggcggcctgatctgtaactcgcgcaaaaccgaccgggaagacgaactgatcatcg ccctggcggagaagcttggcacgcagatgatccacttcgttccccgcgacaacattgtgcagcgcgcggagatccgccggatgacggtgatcgagtacgacccgacctgtcagcaggcgaatgaatatcgtcaactggcgcagaagatcgtcaataacaccaaaaaagtggtgccgacgccgtgcaccatggacga gctggaatcgctgctgatggagttcggcatcatggaagaagaagacaccagcatcattggtaaaaccgccgctgaagaaaacgcggcctga 285 atggttaggaaaagtagaagtaaaaatacaaatatagaactaactgaacatgaccatttattaataagtcaaataaaaaagcttaaaacacaaaccacttgcttttttaataataaaggaggggttgggaagactacattagtagcaaatttaggagcagagctatcaataaactttagtgcaaaagttcttattgtggatgccgaccctcaatgtaatctcacg cagtatgtattaagtgatgaagaaactcaggacttatatgggcaagaaaatccagatagtatttatacagtaataagaccactatcctttggtaaaggatatgaaagtgacctccctataaggcatgtagagaatttcggttttgacataattgtcggtgaccctagacttgctttacaggaagacctttagctggagactggcgagatgccaaaaggcggtgggatg cgaggaattaggacaacttttgtatttgcagagttaattaagaaagctcgtgagctaaattatgattttgttttttgacatgggaccatcattaggcgcaatcaacagggcagtattactggcaatggaattctttgtcgtcccaatgtcaatcgatgtattttcactatgggctattaaaaatttggctccacggtttcaatatggaaaaaaga attagacacagggattcggctctcagaggaacctagcgaattatcacaattatcacctcaaggaaaactaaagtttctcggttacgtcacccaacaacataaagaacgctctggatacgatacaattcagcttgagaatactgaggaagaaataaaatcgaaacgtcgggtaaaggcgtatgaagacattggagaggtgtttccttctaaaattactgag catctttctaaactttatgcatcaaaagatatgaacccacaccttggagatatacgtcatttaggtagtttagctccgaaatcacaatcacaacacgttccgatgatatcagtgtctctggtacaggaaattacaccagacttagaaaaagcgcgcgtgaactttatcgagatattgcaagaagatacttagagaacattcagactgctaatggcgagaaaatag 286 atgaagggaaaggaaattctggcgctgctggacgaacccgcctgcgagcacaaccagaagcaaaaatccggctgcagcgcccctaagcccggcgctaccgccggcggttgcgccttcgacggcgcgcagataacgctcctgcccatcgccgacgtcgcgcacctggtgcacggccccatcggctgcgcgg gcagctcgtgggataaccgcggcagcgtcagcgccggcccggccctcaaccggctcggctttaccaccgatcttaacgaacaggatgtgattatgggccgcggcgaacgccgccgcctgttccacgccgtgcgtcacatcgtcgaccgctatcatccggcggcggtctttatctacaacacctgcgtaccggcgatggag ggcgatgacatcgaggcggtgccaggccgcacagaccgccaccggcgtcccggtcatcgctattgacgccgccggtttctacggcagtaaaaatcttggcaaccgaatggcgggcgacgtgatgctcaggcaggtgattggccagcgcgaaccggccccgtggccagacaacacgccctttgccccggcccagcgccacgata tcggcctgattggcgaattcaatatcgccggcgagttctggcaggtccagccgctgctcgacgagctggggatccgcgtcctcggcagcctctccggcgacggccgctttgccgagatccagaccctgcaccgggcgcaggccaatatgctggtgtgctcgcgcgcgctgatcaacgtcgcccgggggctggagct gcgctacggcacgccgtggtttgaaggcagcttcttacgggatccgcgccacctccgacgccttgcgccagctggcgacgctgctgggggatgacgacctgcgccgccgcaccgaggcgctgatcgcccgcgaagagcaggcggcggagcaggctctgcgccgtggcgtgagcagctccgcgggcgca aagtgctgctctctataccggcggcgtgaaatcctggtcggtggtatcggccctgcaggatctcggcatgaccgtggtggccaccggcacgcgcaaatccaccgaggaggacaaacagcggatccgtgagctgatgggcgacgaggcggtgatgcttgaggagggcaatgcccgcaccctgctcgacgtggtgtaccgctat caggccgacctgatgatcgccggcggacgcaatatgtacaccgcctggaaagcccggctgccgtttctcgatatcaatcaggagcgcgagcacgcctacgccggctatcagggcatcaccctcgcccgccagctctgtctgaccctcgccagccccgtctggccgcaaacgcatacccgcgccccgtggcgctag 287 atgaccaacgcaacaggcgaacgtaaccttgcgctcatccaggaagtcctggaggtgtttcccgaaaccgcgcgcaaagagcgcagaaagcacatgatgatcagcgatccgcagatggagagcgtcggcaagtgcattatctcgaaccgtaaatcgcagcccggggtgatgaccgtgcgcggctgcgcc tatgcgggctcgaaaaggggtggtgtttgggccaatcaaagacatggcccatatctcgcacggccccatcggctgcggccagtattcccgcgccggacggcgcaactactataccggcgtcagcggtgtcgacagcttcggcaccctgaacttccctctgattttcaggagcgcgatattgttttcggcggcgataaaaagctga ccaaactgatcgaagagatggagctgctgttcccgctgaccaaagggatcaccatccagtcggagtgcccggtgggcctgatcggcgatgacatcagcgccgtagccaacgccagcagcaaggcgctggataaaccggtgatcccggtgcgctgcgaaggctttcgcggcgtatcgcaatcgctgggccaccatatcgcca . cccattcgttaagcttaacctcgtccactgctaccgttcgatgaactatatcgcccgccatatggaggagaaacatcagatcccatggatggaatataacttcttcggcccgaccaaaatcgccgaatcgctgcgcaagatcgccgatcaatttgatgacaccattcgcgccaatgcggaagcggtgatcgccaaatatgaggggca gatggcggccatcatcgccaaatatcgccgcggctggaggggcgcaaagtgctgctgtacatgggggggctgcggccgcgccacgtcatcggcgcctatgaggatctcgggatggagatcatcgccgccggctacgagtttgcccataacgatgattacgaccgcaccctgccggacctgaaagagggcaccctgctgtttgac gatgccagcagctatgagctggaggccttcgtcaaagcgctgaaacctgacctcatcggctccgggatcaaagagaaattatcttccagaaaatgggggtgccgttccgccagatgcactcctggggactattccggcccctatcacggctatgacggcttcgccatcttgcccgcgatatggatatgaccctgaacaatccggcg tggaacgaactgactgccccgtggctgaagtctgcgtga 288 atggcagatattatccgcagtgaaaaaccgctggcggtgagcccgattaaaaccgggcaaccgctcggggcgatcctcgccagcctcgggctggcccaggccatcccgctggtccacggcgcccagggctgcagcgccttcgccaaagttttctttattcagcatttccatgacccggtgccgctgcagtcgacgg ccatggatccgaccgccacgatcatggggccgacggcaatatcttcaccgcgctcgacaccctgccagcgccacagcccgcaggccatcgtgctgctcagcaccggtctggcggaagcgcagggcagcgatatcgcccgggtggtgcgccagtttcgcgaggcgcatccgcgccataacggcgtggcgatcct caccgtcaataccccggatttttttggctctatggaaaacggctacagcgcggtgatcgagcagtgggtcgcgccgacgccgcgtccggggcagcggccccggcgggtcaacctgctggtcagccacctctgttcgccaggggatatcgaatggctgggccgctgcgtggaggcctttggcct gcagccggtgatcctgccggacctcgcagtcaatggatggccacctcggtgaaggggattttacgcccctgacccagggcggcgcctcgctgcgccagattgcccagatgggccagagtctgggcagcttcgccattggcgtgtcgctccagcgggcggcatcgctcctgacccaacgcagccgcggcgacgt gatcgccctgccgcatctgatgaccctcgaccattgcgatacctttatccatcagctggcgaagatgtccggacgccgcgtaccggcctggattgagcgccagcgtggccagctgcaggatgcgatgatcgactgccatatgtggcttcagggccagcgcatggcgatggcggcggagggcgacctgctggcggcgtgg tgtgatttcgcccgcagccaggggatgcagcccggcccgctggtcgcccccaccagccaccccagcctgcgccagctgccggtcgagcaagtcgtgccgggggatcttgaggatctgcagcagctgctgagccaccaacccgccgatctgctggtggctaactctcacgcccgcgatctggcggagcagtttgccctg ccgctgatccgcgtcggttttcccctcttcgaccggctcggtgagtttcgtcgcgtccgccaggggtacgccggtatgcgagatacgctgtttgaactggccaatctgctgcgcgaccgccatcaccacaccgccctctaccgctcgccgcttcgccagggcgccgacccccagccggcttcaggagacgcttatg ccgcccattaa 289 atgagccaaacgatcgataaaattcacagctgttatccgctgtttgaacaggatgaataccagaccctgttccagaataaaaagacccttgaagaggcgcacgacgcgcagcgtgtgcaggaggttttgcctggaccaccaccgccgagtatgaagcgctgaacttccagcgcgaggcgctgaccgtcgacccggccaaag cctgccagccgctcggcgccgtactctgcgcgctggggttcgccggcaccctgccctacgtgcacggctcccagggctgcgtcgcctattttcgcacctactttaaccgccattttaaagagccggtcgcctgcgtctccgactccatgaccgaggacgcggcggtgttcggcggcaacaacaacatgaatctggg cctgcagaatgccagcgcgctgtataaacccgagattatcgccgtctccaccacctgtatggccgaggtgatcggcgacgatctgcaggcgtttcgccaacgccaaaaaagagggattgttgacgaccgcatcgccattccttacgcccatacccccagctttatcggcagccatgtcaccggctgggacaatatgttcgaaggg ttcgcgaagacctttaccgctgactacgccgggcagccgggcaaacagcaaaagctcaatctggtgaccggatttgagacctatctcggcaacttccgcgtgctgaagcggatgatggcgcagatggatgtcccgtgcagcctgctctccgacccatcagaggtgctcgacaccccgccgacggccattaccggatgtacgccggc ggcaccagccagcaggagatcaaaaccgcgccggacgccattgacaccctgctgctgcagccgtggcagctggtgaaaagcaaaaaggtggttcaggagatgtggaaccagcccgccaccgaggtggccgttccgctgggcctggccgccaccgacgcgctgctgatgaccgtcagtcagctgaccggcaaaccgatcg ccgacgctctgaccctggagcgcggccggctggtcgacatgatgctggattcccacacctggctgcatggcaaaaaattcggcctctacggcgatccggatttcgtgatggggctgacgcgcttcctgctggagctgggctgcgagccgacggtgatcctcagtcataacgccaataaacgctggcaaaaagcgatgaaga aaatgctcgatgcctcgccgtacggtcaggaaagcgaagtgttcatcaactgcgacctgtggcacttccggtcgctgatgttcacccgtcagccggactttatgatcggtaactcctacggcaagtttatccagcgcgataccctggcaaagggcaaagccttcgaagtgccgctgatccgtctgggctttccgctgtt cgaccgccatcatctgcaccgccagaccacctggggctatgaaggcgcaatgaacatcgtcacgacgctggtgaacgccgtgctggaaaaactggaccacgacaccagccagttgggcaaaaccgattacagcttcgacctcgttcgttaa 290 atgatgccgctttctccgcaattacagcagcactggcagacggtcgctgaccgtctgccagcggattttcccattgccgaactgagcccacaggccaggtcggtcatggcgttcagcgattttgtcgaacagagtgtgatcgcccagccgggctggctgaatgagcttgcggactcctcgccggaggcggaagagtggcggcat tacgaggcctggctgcaggatcgcctgcaggccgtcactgacgaagcggggttgatgcgagagctgcgtctcttccgccgccagatgatggtccgcatcgcctgggcgcaggcgctgtcgctggtgagcgaagaagagagactctgcagcagctgagcgtcctggcggagaccctgattgtcgccgcccgcgact ggctgtacgccgcctgctgtaaggagtggggaacgccatgcaatgccgagggccagccgcagccgctgctgatcctcgggatgggaaagctgggcggcggcgagctgaacttctcttccgatatcgatctgatctttgcctggcctgagcatggcgccacccgcggcggccgccgcgagctggataacgcccagttcttt acccgtctggggcagcggctgatcaaggcccttgaccagccgacgcaggacggctttgtctatcgggttgacatgcgcctgcggccgttggcgacagtgggccgctggtactcagtttgcggcgctggaagattattaccaggagcagggtcgggactgggaacgctatgcgatggtgaaagcgcggatcatgggcgataacg . tatcgttcaggtctttcaactgatccgcggtggtcgcgaacctgcactgcagcagcgcgccctgctgccgacgctggcggcgattgatgagctacatctgctgccggaaggcgacgcggcgctgctgcgcgaggcctatctgttcctgcgccggctggaaaacctgctgcaaagcatcaacgatgagcag acccagaccctgccgcaggatgaacttaaccgcgccaggctggcgtgggggatgcataccgaagactgggagacgctgagcgcgcagctggcgagccagatggccaacgtgcggcgagtgtttaatgaactgatcggcgatgatgaggatcagtccccggatgagcaactggccgagtactggcgcgagctgtggcaggatgcgct ggaagaagatgacgccagcccggcgctggcgcatttaaacgataccgaccgccgtagcgtgctggcgctgattgccgattttcgtaaagagctggatcggcgcaccatcggcccgcgcggccgccaggtgctggatcagctgatgccgcatctgctgagcgaaatctgctcgcgcgccgatgcgccgctgcctct ggcgcggatcacgccgctgttgaccgggatcgtcacccgtaccacctatcttgagctgctgagcgaattccccggcgcgctgaagcacctgatcacgctctgcgcggcgtcgccgatggtcgccagccagctggcgcgccacccgctgctgctggatgagctgctggatcccaacaccctctatcagccgacgg cgaccgatgcctatcgcgacgagctgcgccagtacctgctgcgcgtgccggaagaggatgaagagcagcagctggaggcgttgcgccagtttaagcaggcgcagcagctgcatatcgcggcggcggatatcgctggtaccctgccggtgatgaaggtcagcgatcacttaacctggcttgccgaagcgatcct cgacgcggtggtgcagcaggcatgggggcagatggtcgctcgctacggccagccgacccacctgcacgatcgccagggtcgcggcttcgccgtcgtcggctacggtaagcttggcggctgggagctgggctacagctccgatctcgatctggtgttcctccatgactgcccggcggaggtgatgaccgacggcga gcgggagattgacggccgtcagttctacctgcggctggcccagcggatcatgcacctgttcagcacccgcacctcgtccggtattctctacgaagtggacgcccggctgcgtccttctggcgcggcggggatgctggtcaccaccgccgacgcgtttgctgactatcagcagcagaacgaagcctggacgtgggaacatcaggcg ctggtgcgcgcccgcgtggtctatggcgacccggcgctgcaggcgcgctttgacgccattcgtcgcgatatcctgaccaccccgcgggaggggatgaccctgcagaccgaggttcggagatgcgcgagaagatgcgcgcccaccttggcaacaaacatcccgatcgttttgatatcaaagccgatgccgg cgggatcaccgatattgaatttattactcagtatctggtcctacgctatgccagtgacaagccgaagctgacccgctggtctgacaacgtgcgtattcttgagctgctggcgcagaacgacatcatggacgaggaggaggcgcgcgccttaacgcatgcgtacaccaccttgcgtgatgcgctccatcacctggccctgcaggagcag ccgggacacgtggcgccagaggccttcagccgggagcgtcagcaggtcagcgccagctggcagaagtggctgatggcttaa 291 agtctgaactcatcctgcggcagtcggtgagacgtatttttgaccaaagagtgatctacatcacggaatttgtggttgttgctgcttaaaagggcaaatctacccttagaatcaactgttatatcagggggattcagagagatattaggaatttgcacaagcgcacaatttaaccacatcatgataacgccatgtaaaaaaagataaaa aaacaaaatgcagtgacttacatcgcaagcaaggcattttcttatccaattgctcaaagtttggcctttcatatcgcaacgaaaatgcgtaatatacgcgcccttgcggacatcagtatggtcattcctagttcatgcgcatcggacaccaccagcttacaaattgcctgattgcggccccgatggccggtatcactgaccgaccat ttcgtgccttatgtcatgcgatgggggctgg 292 tgaacatcactgatgcacaagctacctatgtcgaagaattaactaaaaaactgcaagatgcaggcattcgcgttaaagccgacttgagaaatgagaagattggctttaaaattcgcgaacacacgctacgccgtgttccttatatgttagtttgtggcgataaagaggtcgaagcaggcaaagttgctgttcgtacccgcc gcggcaaagacttaggaagcatggatgttagcgaagtcgttgacaaactgctggcggaaatccgcagcagaagtcttcatcaactggaggaataaagtattaaaggcggaaaacgagttcaaccggcgcgtcctaatcgcattaacaaagagattcgcgcgcaagaagttcgcctcacaggcgtcgatggcgagcagattggtatt gtcagtctgaatgaagctcttgaaaaagctgaggaagcgggcgtcgatttagtagaaatcagtccgaatgccgagccgccagtttgtcgaatc 293 tacagtagcgcctctcaaaatagataaacggctcatgtacgtgggccgtttattttttctacccataatcgggaaccggtgttataatgccgcgccctcatattgtggggatttcttaatgacctatcctgggtcctaaagttgtagttgacattagcggagcactaac 294 aattttttttcacaaagcgtagcgttattgaatcgcacattttaaactgttggccgctgtggaagcgaatattggtgaaaggtgcggttttaaggcctttttctttgactctctgtcgttacaaagttaatatgcgcgccct 295 ttaaaaacgtgaccacgagcattaataaacgccacgaaatgtggcgtttatttattcaaaaagtatcttctttcataaaaagtgctaaatgcagtagcagcaaaattgggataagtcccatggaatacggctgttttcgctgcaatttttaactttttcgtaaaaaaagatgtttctttgagcgaacgatcaaa atatagcgttaaccggcaaaaaattattctcattagaaaaatagtttgtgtaatacttgtaacgctacatggagattaacttaatctagagggttttata 296 atggcgctcaaacagttaatccgtctgtgtgccgcctcgccgatggtcgcgacacaacttgcacgtcatcctttattgctcgatgaactgctcgacccgcgcacgctttaccagccgattgagccgggcgcttaccgcgacgaactgcgtcagtatctgatgcgggtgccaacagaagacgaagaacagcagct tgaagccgtgcgccagttcaaacaggcccagcatttgcgtatcgcagccggggatatttccggggcattgccggtgatgaaagtcagtgaccatttaacctaccttgccgaggccattctcgatgtcgtggtgcagcatgcgtgggaacaaatggtcgtaaaatacgggcagcccgcgcatcttcagcaccgtga ggggcgcggttttgccgtggtcggttacgggaaactcggtggctgggagctgggttatagctcagatctggatctggtcttcctgctcgattgcgcgccggaggtgatgacggacggcgaacgcagcatcgacggacgtcagttttatcttcggctggcgcagcgcattatgcacttattcagcacccggacatcgtca ggcattctttacgaggttgatccgcgtctgcgaccttccggcgcatccggcatgctggtcagtaccattgaagcgtttgcagattatcaggccaatgaagcctggacgtgggagcatcaggcgctggttcgcgcgcgcgtggtttacggggatccgcaactgacacagcaatttaacgccacgcgtcgcgacattctttgccgcca gcgcgatggcgacggcctgcgtaaggaggtccgtgaaatgcgcgagaaaatgtatgcccatctggggagtaaaaaagcccacgagtttgatctgaaagccgatccgggtggcatcacggatattgaattcattgcacaatacctggttctgcgtttcgcgcatgatgagccgaagctgacgcgctggtctgataac gtgcggatttttgaactgatggcacgatatgacatcatgccggaagaggaagcgcgccatctgacgcaggcttatgtgacgctgcgcgatgaaattcatcatctggcgttgcaggaacacagcgggaaagtggccgcggacagctttgctactgagcgcgcgcagatccgtgccagctgggcaaagtggctcggctga 297 cggtactggaacagaaatcggcggatgcgcaggaaatttgttatgacacggcctgtctgaagtgcaagttagtgcttacttcctggctggcaacctcaggctggacgccgtttattgatgataaatctgcgaagaaactggacgcttccttcaaacgttttgctgacatcatgctcggtcgtaccgcagcggatctgaaagaagcct ttgcgcagccactgacggaagaaggttatcgcgatcagctggcgcgcctgaaacgccagatcattaccttccatttgcttgccggtgcttaccctgaaaaagacgtcgatgcgtatattgccggctgggtggacctgcaacaggccatcgttcagcagcaacacgcctgggaggattcggcccgttctcacgcggt gatgatggatgctttctggttaaacgggcaacctcgttaactgactgactagcctgggcaaactgcccgggctttttttgcaaggaatctgatttcatggcgctcaaacagttaatccgtctgtgtgccgcctcgccgatggtcgcgacacaacttgcacgtcatcctttattgctcgatgaactgctcgacccgcgc acgctttaccagccgattgagccgggcgcttaccgcgacgaactgcgtcagtatctgatgcgggtgccaacagaagacgaagaacagcagcttgaagccgtgcgccagttcaaacaggcccagcatttgcgtatcgcagccggggatatttccggggcattgccggtgatgaaagtcagtgaccatttaacctaccttgccgagg ccattctcgatgtcgtggtgcagcatgcgtgggaacaaatggtcgtaaaatacgggcagcccgcgcatcttcagcaccgtgaggggcgcggttttgccgtggtcggttacgggaaactcggtggctgggagctgggttatagctcagatctggatctggtcttcctgctcgattgcgcgccggaggtgatg acggacggcgaacgcagcatcgacggacgtcagttttatcttcggctggcgcagcgcattatgcacttattcagcacccggacatcgtcaggcattctttacgaggttgatccgcgtctgcgaccttccggcgcatccggcatgctggtcagtaccattgaagcgtttgcagattatcaggccaatgaagcctggacgtgggagcatcagg cgctggttcgcgcgcgcgtggtttacggggatccgcaactgacacagcaatttaacgccacgcgtcgcgacattctttgccgccagcgcgatggcgacggcctgcgtaaggaggtccgtgaaatgcgcgagaaaatgtatgcccatctggggagtaaaaaagcccacgagtttgatctgaaagccgat ccgggtggcatcacggatattgaattcattgcacaatacctggttctgcgtttcgcgcatgatgagccgaagctgacgcgctggtctgataacgtgcggatttttgaactgatggcacgatatgacatcatgccggaagaggaagcgcgccatctgacgcaggcttatgtgacgctgcgcgatgaaattcatcatctggcgttg caggaacacagcgggaaagtggccgcggacagctttgctactgagcgcgcgcagatccgtgccagctgggcaaagtggctcggctgagggtttttattcggctaacaggcgcttgtgatattatccggcgcattgtatttacccgatttgatttatctgttttggagtcttgggatgaaagtgactttgcctgattttcacc gcgcaggtgtgctggttgtcggtgacgtaatgttagaccgttactggtatggcccgaccaatcgtatttctccggaagctccggtgccggtggtgaaggtcagtaccattgaagagcggcctggcggtgcagctaacgtggcgatgaacatttcatctctgggcgcctcttcctgtctgatcggcctgaccggcgtagac gacgctgcgcgtgccctcagtgagcgtctggcagtgagcgtctggcagaagtgaaagttaactgcgatttcgtcgcactatccacacatcctaccatcaccaaactgcgaattttgtcccgtaaccagcaactgatccgcctcgactttgaggaaggttttgaaggcgttgatctcgagccgatgctgaccaaaataga 298 atgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggttgaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaac caccgtctgctggagtctgaactcatcctgcgatgggggctgggccgtctctgaagctctcggtgaacattgttgcgaggcaggatgcgagctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgcgttatgcccgcccggaagcggcgttttcccgtccggggaatggcatggagctgcgccttat ccagacgctgatcgcccatcatcgcggttctttagatctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacagcaggttatcaccggaggcttaaaatga 299 tgtttcgtctcgaggccgggcaactgagcggccccgttgaaaccgacctgggctggcatctgttgttgtgcgaacaaattcgcctgccgcaacccttgccgaaagccgaagccttaacgcgggtgcgtcagcaactgattgcccggcaacagaaacattatcagcgccagtggctgcaacaactgatcaacgcct gagcctgttctccttcttgttgatgcagacgggttaatgcccgttttgcacgaaaaatgcacataaattgcctgcgttgccttataacagcgcagggaaatcctgcctccggccttgtgccacaccgcgctttgcctggtttgtggtaaaaactggcccgctttgcatcctgatgcttaaaacaccccgttca gatcaacctttgggcagataagcccgcgaaaggcctgcaaattgcacggttattccgggtgagtatatgtgtgatttgggttccggcattgcgcaataaaggggagaaagacatgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggtt gaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaaccaccgtctgctggagtctgaactcatcctgcgatgggggctgggccgtctctctgaagctctcggtgaacattgttgcgaggcaggatgcgag ctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgcgttatgcccgcccggaagcggcgttttcccgtccggggaatggcatggagctgcgccttatccagacgctgatcgcccatcatcgcggttctttagatctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacagca ggttatcaccgggaggcttaaaatgacccagttacctaccgcggcccggttatccggcgctttgatatgtctgcccagtttacggcgctttatcgcatcagcgtggcgctgagtcaggaaagcaacaccggcgcgcactggcggcgatcctcgaagtgcttcacgatcatgcatttatgcaatacggcatggtgtg tctgtttgataaagaacgcaatgcactctttgtggaatccctgcatggcatcgacggcgaaaggaaaaaagagacccgccatgtccgttaccgcatgggggaaggcgtgatcggcgcggtgatgagccagcgtcaggcgctggtgttaccgcgcatttcagacgatcagcgttttctcgaccgcctgaatatttac gattacagcctgccgttgattggcgtgccgatccccggtgcggataatcagccatcgggcgtgctggtggcacagccgatggcgttgcacgaagaccggctgactgccagtacgcggtttttagaaatggtc 300 atgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggttgaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaac caccgtctgctggttaaagcctgccggtacagcaggttatcaccgggaggcttaaaatga 301 tgtttcgtctcgaggccgggcaactgagcggccccgttgaaaccgacctgggctggcatctgttgttgtgcgaacaaattcgcctgccgcaacccttgccgaaagccgaagccttaacgcgggtgcgtcagcaactgattgcccggcaacagaaacattatcagcgccagtggctgcaacaactgatcaacgcct gagcctgttctccttcttgttgatgcagacgggttaatgcccgttttgcacgaaaaatgcacataaattgcctgcgttgccttataacagcgcagggaaatcctgcctccggccttgtgccacaccgcgctttgcctggtttgtggtaaaaactggcccgctttgcatcctgatgcttaaaacaccccgttca gatcaacctttgggcagataagcccgcgaaaggcctgcaaattgcacggttattccgggtgagtatatgtgtgatttgggttccggcattgcgcaataaaggggagaaagacatgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggtt gaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaaccaccgtctgctggttaaagcctgccggtacagcaggttatcaccggaggcttaaaatgaccgttacctaccgcgggccggttatccggc gctttgatatgtctgcccagtttacggcgctttatcgcatcagcgtggcgctgagtcaggaaagcaacaccgggcgcgcactggcggcgatcctcgaagtgcttcacgatcatgcatttatgcaatacggcatggtgtgtctgtttgataaagaacgcaatgcactctttgtggaatccctgcatggcatcgacggc gaaaggaaaaaagagacccgccatgtccgttaccgcatgggggaaggcgtgatcggcgcggtgatgagccagcgtcaggcgctggtgttaccgcgcatttcagacgatcagcgttttctcgaccgcctgaatatttacgattacagcctgccgttgattggcgtgccgatccccggtgcggataatcagccatc gggcgtgctggtggcacagccgatggcgttgcacgaagaccggctgactgccagtacgcggtttttagaaatggtc 302 atgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggttgaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaac caccgtctgctggagtctgaactcatcctgcggcagtcggtgagacgtatttttgaccaaagagtgatctacatcacggaattttgtggttgttgctgcttaaaagggcaaatctacccttagaatcaactgttatatcagggggattcagagagatattaggaatttgcacaagcgcacaatttaaccacatcatgataacgccatgtaaaaca aagataaaaaaacaaaatgcagtgacttacatcgcaagcaaggcattttcttatccaattgctcaaagtttggcctttcatatcgcaacgaaaatgcgtaatatacgcgcccttgcggacatcagtatggtcattcctagttcatgcgcatcggacaccaccagcttacaaattgcctgattgcggccccgatggccggtatc actgaccgaccatttcgtgccttatgtcatgcgatggggggctgggccgtctctgaagctctcggtgaacattgttgcgaggcaggatgcgagctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgcgttatgcccgcccggaagcggcgttttcccgtccggggaatggcatggagctgcgcctt atccagacgctgatcgcccatcatcgcggttctttagatctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacagcaggttatcaccggaggcttaaaatga 303 tgtttcgtctcgaggccgggcaactgagcggccccgttgaaaccgacctgggctggcatctgttgttgtgcgaacaaattcgcctgccgcaacccttgccgaaagccgaagccttaacgcgggtgcgtcagcaactgattgcccggcaacagaaacattatcagcgccagtggctgcaacaactgatcaacgcct gagcctgttctccttcttgttgatgcagacgggttaatgcccgttttgcacgaaaaatgcacataaattgcctgcgttgccttataacagcgcagggaaatcctgcctccggccttgtgccacaccgcgctttgcctggtttgtggtaaaaactggcccgctttgcatcctgatgcttaaaacaccccgttca gatcaacctttgggcagataagcccgcgaaaggcctgcaaattgcacggttattccgggtgagtatatgtgtgatttgggttccggcattgcgcaataaaggggagaaagacatgagcatcacggcgttatcagcatcatttcctgaggggaatatcgccagccgcttgtcgctgcaacatccttcactgttttataccgtggtt gaacaatcttcggtggcgatttcgctgaccgatccgcaggcgcgcatttgttatgccaatccggcattctgccgccagacgggttttgcacttgagacacttttgggcgagaaccaccgtctgctggagtctgaactcatcctgcggcagtcggtgagacgtatttttgaccaaagagtgatctacatcacggaattttgtggtt gttgctgcttaaaagggcaaatctacccttagaatcaactgttatatcagggggattcagagagatattaggaatttgcacaagcgcacaatttaaccacatcatgataacgccatgtaaaacaaagataaaaaaacaaaatgcagtgacttacatcgcaagcaaggcattttcttatccaattgctcaaagtttggcctttcatatcgcaacga aaatgcgtaatatacgcgcccttgcggacatcagtatggtcattcctagttcatgcgcatcggacacccaccagcttacaaattgcctgattgcggccccgatggccggtatcactgaccgaccatttcgtgccttatgtcatgcgatggggggctgggccgtctctgaagctctcggtgaacattgttgcgaggcaggatgcga gctggttgtgttttgacattaccgataatgtgccgcgtgaacgggtgcgttatgcccgcccggaagcggcgttttcccgtccggggaatggcatggagctgcgccttatccagacgctgatcgcccatcatcgcggttctttagatctctcggtccgccctgatggcggcaccttgctgacgttacgcctgccggtacag caggttatcaccgggaggcttaaatgacccagttacctaccgcggcccggttatccggcgctttgatatgtctgcccagtttacggcgctttatcgcatcagcgtggcgctgagtcaggaaagcaacaccgggcgcgcactggcggcgatcctcgaagtgcttcacgatcatgcatttatgcaatacggcatggtgt gtctgtttgataaagaacgcaatgcactctttgtggaatccctgcatggcatcgacggcgaaaggaaaaaagagacccgccatgtccgttaccgcatgggggaaggcgtgatcggcgcggtgatgagccagcgtcaggcgctggtgttaccgcgcatttcagacgatcagcgttttctcgaccgcctgaatattt acgattacagcctgccgttgattggcgtgccgatccccggtgcggataatcagccatcgggcgtgctggtggcacagccgatggcgttgcacgaagaccggctgactgccagtacgcggttttagaaatggtc

[0389] The use of terms in the singular within the scope of the description of the invention (especially within the scope of the following claims) should be considered as referring to the singular and plural, unless otherwise indicated herein or clearly dictated by the context. Unless otherwise indicated, the terms "comprising", "having", "including", and "comprising" should be considered as open terms (ie, meaning "including as non-limiting examples"). Unless otherwise indicated herein, the indication of ranges of values in this document serves solely as a way of shortening the individual indication of each individual value that is in the specified range, and each individual value is included in the description as if it were individually indicated in this document. For example, if the range is 10-15, then 11, 12, 13, and 14 are also included. Unless otherwise indicated herein or otherwise explicitly dictated by the context, all methods described herein can be performed in any suitable order. Unless otherwise indicated, the use of any and all examples or illustrative language (eg, "such as") given herein is intended solely to better describe the invention and is not intended to limit the scope of the invention. None of the language in the description should be taken as an indication that any non-claimed element is essential to the practice of the invention.

[0390] While preferred embodiments of the present invention are provided and described herein, those skilled in the art will appreciate that such embodiments are provided by way of example only. Many variations, changes and modifications can be made by those skilled in the art without deviating from the invention. It should be understood that various alternatives to the embodiments of the invention described herein can be used in the practice of the invention. It is to be understood that the scope of the invention is defined by the following claims and that the methods and structures within the scope of those claims and their equivalents are thus encompassed.

ITEMS

1. A method for increasing nitrogen fixation in a non-legume plant, comprising:

a. applying to a plant a plurality of non-intergeneric bacteria, wherein said plurality includes non-intergeneric bacteria that:

i. have an average colonizing capacity per unit of plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue; and/or

ii. produce a fixed N in an amount of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour, and

where a plurality of non-intergeneric bacteria, in planta , produce 1% or more of fixed nitrogen in the plant, and

wherein each member of a plurality of non-intergeneric bacteria carries at least one genetic variation introduced into at least one gene or non-coding polynucleotide of the genetic regulatory network for nitrogen fixation or assimilation such that the non-intergeneric bacteria become capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen .

2. The method of claim 1, wherein the plurality of non-intergeneric bacteria comprises bacteria that: have an average colonizing capacity per unit plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue.

3. The method of claim 1, wherein the plurality of non-intergeneric bacteria comprises bacteria that: produce a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour.

4. The method of claim 1, wherein the plurality of non-intergeneric bacteria comprises bacteria that: have an average colonizing capacity per unit plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue and produce a fixed N in an amount of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour.

5. The method of claim 1, wherein at least one genetic variation comprises an introduced control sequence operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

6. The method of claim 1, wherein at least one genetic variation comprises a promoter operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

7. The method of claim 1, wherein at least one genetic variation comprises an inducible promoter operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

8. The method of claim 1, wherein the plurality of non-intergeneric bacteria do not contain a constitutive promoter operably linked to a gene for a nitrogen fixation or assimilation genetic regulatory network.

9. The method of claim 1, wherein the plurality of non-intergeneric bacteria do not contain a constitutive promoter operably linked to a gene in the nif gene cluster.

10. The method of claim 1, wherein a plurality of non-intergeneric bacteria, in planta , excrete nitrogen-containing nitrogen fixation products.

11. The method of claim 1 wherein the plurality of non-intergeneric bacteria applied to the plant does not promote increased uptake of exogenous non-atmospheric nitrogen.

12. The method of claim 1, wherein the plant is grown in field soil to which at least about 22.7 kg of nitrogen-containing fertilizer per acre has been added, and wherein the nitrogen-containing fertilizer contains at least about 5% nitrogen by weight.

13. The method of claim 1, wherein the plant is grown in field soil to which at least about 22.7 kg of nitrogen-containing fertilizer has been added per acre, and wherein the nitrogen-containing fertilizer contains at least about 5% nitrogen by mass, and wherein the nitrogen-containing fertilizer contains ammonium or an ammonium-containing molecule.

14. The method of claim 1 wherein the exogenous nitrogen is selected from a fertilizer containing one or more of: glutamine, ammonia, ammonium, urea, nitrate, nitrite, ammonium-containing molecules, nitrate-containing molecules, and nitrite-containing molecules.

15. The method of claim 1, wherein the plurality of non-intergeneric bacteria, in planta , produce 5% or more of fixed nitrogen in the plant.

16. The method of claim 1, wherein the plurality of non-intergeneric bacteria, in planta , produce 10% or more fixed nitrogen in the plant.

17. The method of claim 1, wherein the plurality of non-intergeneric bacteria, in planta , fixes atmospheric nitrogen under non-nitrogen-limiting conditions.

18. The method of claim 1, wherein a plurality of non-intergeneric bacteria, in planta , excrete nitrogen-containing nitrogen fixation products.

19. The method of claim 1 wherein the fixed nitrogen produced by a plurality of non-intergeneric bacteria is determined by diluting the enriched fertilizer with atmospheric N ₂ gas in plant tissue.

20. The method of claim 1, wherein at least one gene or non-coding polynucleotide of the nitrogen fixation or assimilation genetic regulatory network is selected from the group consisting of: nifA, nifL, ntrB, ntrC, a polynucleotide encoding glutamine synthetase , glnA, glnB, glnK, Drat, AMTB , polynucleotide, encoding glutamine, GLND, GLNE, NIFJ, NIFH, NIFD, NIFK, NIFY, NIFE, NIFN, NIFU, NIFS, NIFV, NIFW, NIFZ, NIFM, NIFF, NIFB, NIFQ and Gene associated With biosynthesis nitrogenase enzyme.

21. The method according to p. 1, where at least one genetic variation is a mutation that leads to one or more of: increased expression or activity of NifA or glutaminase; reduced expression or activity of nifL, NtrB, glutamine synthetase, GlnB, GlnK, DraT, AmtB; reduced GlnE adenylyl scavenging activity; or reduced GlnD uridylate scavenging activity.

22. The method according to p. 1, where at least one genetic variation is selected from: (A) knockout mutations; (B) removing or eliminating the regulatory sequence of the target gene; (C) the presence of a heterologous regulatory sequence insertion or (D) a domain deletion.

23. The method of claim 1, wherein the at least one genetic variation is a mutant nifL gene that is altered to contain a heterologous promoter inserted into said nifL gene.

24. The method of claim 1, wherein the at least one genetic variation is a mutant glnE gene that results in a truncated glnE protein lacking an adenylyl-removing (AR) domain.

25. The method of claim 1, wherein the at least one genetic variation is a mutant amtB gene that results in a lack of expression of said amtB gene.

26. The method according to p. 1, where at least one genetic variation is selected from: a mutant nifL gene that is modified to contain a heterologous promoter built into the specified nifL gene; a mutant glnE gene that results in a truncated glnE protein lacking an adenylyl-removal (AR) domain; a mutant amtB gene that results in no expression of said amtB gene; and their combinations.

27. The method of claim 1, wherein the at least one genetic variation is a mutant nifL gene that is altered to contain a heterologous promoter inserted into said nifL gene and a mutant glnE gene that results in a truncated glnE protein with no adenylyl-removing (AR) domain.

28. The method of claim 1, wherein at least one genetic variation is a mutant nifL gene that is altered to contain a heterologous promoter inserted into said nifL gene and a mutant glnE gene that results in a truncated glnE protein with no adenylyl-removing (AR) domain, and a mutant amtB gene that results in the absence of said amtB gene expression.

29. The method of claim. 1, where the plant contains seeds, stem, flower, fruit, leaves or rhizome.

30. The method of claim 1 wherein the plurality of non-intergeneric bacteria are formulated into a composition.

31. The method of claim 1, wherein the plurality of non-intergeneric bacteria are formulated into a composition containing an agriculturally acceptable carrier.

32. The method of claim 1, wherein a plurality of non-intergeneric bacteria are introduced into the furrows in which seeds of said plant are planted.

33. The method of claim 1 wherein the plurality of non-intergeneric bacteria is formulated into a liquid furrow composition containing the bacteria at a concentration of from about 1 x 10 ⁷ to about 1 x 10 ¹⁰ cfu per milliliter.

34. The method of claim 1, wherein a plurality of non-intergeneric bacteria is applied to the seeds of said plant.

35. The method of claim 1, wherein the plurality of non-intergeneric bacteria are formulated into a seed coat and applied to the seed of said plant.

36. The method of claim 1 wherein a plurality of non-intergeneric bacteria are formulated into a seed coat and applied to the seeds of said plant at a concentration of about 1 x 10 ⁵ to about 1 x 10 ⁷ CFU per seed.

37. The method of claim 1 wherein the plant is a cereal crop.

38. The method of claim. 1, where the plant is selected from the group consisting of: corn, rice, wheat, barley, sorghum, millet, oats, rye and triticale.

39. The method of claim 1 wherein the plant is corn.

40. The method of claim 1 wherein the plant is a crop.

41. The method of claim 1, wherein the plant is a genetically modified organism.

42. The method of claim. 1, where the plant is not a genetically modified organism.

43. The method of claim 1 wherein the plant has been genetically engineered or bred to use nitrogen efficiently

44. The method of claim 1, wherein the plurality of non-intergeneric bacteria comprises at least two different species of bacteria.

45. The method of claim 1 wherein the plurality of non-intergeneric bacteria comprises at least two different strains of the same bacterial species.

46. The method of claim 1 wherein the plurality of non-intergeneric bacteria comprises bacteria selected from: Rahnella aquatilis , Klebsiella variicola , Achromobacter spiritinus , Achromobacter marplatensis , Microbacterium murale , Kluyvera intermedia , Kosakonia pseudosacchari , Enterobacter species, Azospirillum lipoferum , Kosak onia sacchari and their combinations.

47. The method of claim 1, wherein the plurality of non-intergeneric bacteria are endophytic, epiphytic, or rhizospheric.

48. The method of claim 1, wherein the plurality of non-intergeneric bacteria comprises bacteria selected from: bacteria deposited as PTA-122293, bacteria deposited as PTA-122294, bacteria deposited as NCMA 201701002, bacteria deposited as NCMA 201708004, bacteria deposited as NCMA 201708003, bacteria deposited as NCMA 201708002, bacteria deposited as NCMA 201712001, bacteria deposited as NCMA 201712002, and combinations thereof.

49. Bacterial composition containing:

at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system fertilized with more than 9.1 kg of nitrogen per acre.

50. Bacterial composition containing:

at least one strain of bacteria that has been bred to fix atmospheric nitrogen in an agricultural system that has been fertilized with more than 9.1 kg of nitrogen per acre.

51. A bacterial composition according to claim 49 or claim 50, wherein said fertilizer is a chemical fertilizer selected from the group consisting of anhydrous ammonia, ammonium sulfate, urea, ammonium diphosphate, ureaform, UAN (urea ammonium nitrate), ammonium monophosphate , ammonium nitrate, nitrogen solutions, calcium nitrate, potassium nitrate and sodium nitrate.

52. Bacterial composition containing:

at least one genetically engineered bacterial strain that fixes atmospheric nitrogen, at least one genetically engineered bacterial strain containing exogenously added DNA, where said exogenously added DNA is at least 80% identical with the corresponding native bacterial strain.

53. The bacterial composition of claim 52, wherein said exogenously added DNA is at least 85% identical to the corresponding native bacterial strain.

54. The bacterial composition of claim 52, wherein said exogenously added DNA is at least 90% identical to the corresponding native bacterial strain.

55. The bacterial composition of claim 52, wherein said exogenously added DNA is at least 95% identical to the corresponding native bacterial strain.

56. The bacterial composition of claim 52, wherein said exogenously added DNA is at least 99% identical to the corresponding native bacterial strain.

57. The bacterial composition of claim 52, wherein said exogenously added DNA originates from the same bacterial strain as said corresponding native bacterial strain.

58. A bacterial composition according to any of the preceding claims, wherein said bacterial composition is a fertilizer composition.

59. A bacterial composition according to any one of the preceding claims, wherein said at least one genetically engineered bacterial strain contains at least one variation in a gene or intergenic region within 10,000 bp. from a gene selected from the group consisting of: nifA , nifL , ntrB , ntrC , glnA , glnB , glnK , draT , amtB , glnD , glnE , nifJ , nifH , nifD , nifK , nifY , nifE , nifN , nif U , nifS , nifV , nifW , nifZ , nifM , nifF , nifB , and nifQ .

60. A bacterial composition according to any one of the preceding claims, further comprising at least one additional component selected from the group consisting of a tackifying agent, a microorganism stabilizer, a fungicide, an antibacterial agent, a preservative, a stabilizer, a surfactant, an anti-complex agent, herbicide, nematocide, insecticide, plant growth regulator, fertilizer, rodenticide, desiccant, bactericide and nutrient.

61. A bacterial composition according to any one of the preceding claims, further comprising at least one additional component selected from the group consisting of a dye, a primer, a granule, a disinfectant, a plant growth regulator, a preservative, and a nematocide.

62. A bacterial composition according to any one of the preceding claims, wherein said bacterial composition is formulated for field application.

63. The bacterial composition of claim 62, wherein said bacterial composition is formulated to be applied to the furrow.

64. The bacterial composition of claim 62, wherein said bacterial composition is formulated for application as a seed coat, seed dressing, or seed treatment.

65. The bacterial composition of claim 61 or claim 62, wherein said bacterial composition is formulated to be applied during, before, or after planting the seeds.

66. Seed composition containing plant seeds that are inoculated with a bacterial composition according to any one of the preceding paragraphs.

67. A method of growing an agricultural crop using a plurality of seeds with a seed composition according to claim 66.

68. The method of claim 67, further comprising harvesting said crop.

69. The method of making a bacterial composition according to any one of the preceding paragraphs. in field.

70. The method of claim 69, wherein said bacterial composition is applied to said field in a form selected from the group consisting of liquid form, dry form, granules, powder, and pellet.

71. The method of claim 69, wherein said bacterial composition is applied to said field as a seed coat, seed dressing, or seed dressing.

72. The method of claim 69, wherein said bacterial composition is applied to said field as a furrow treatment.

73. The method of claim 69, wherein said bacterial composition is applied to said field during, before, or after planting the seeds.

74. A fertilizer composition containing a bacterial composition according to any one of the preceding paragraphs.

75. A fertilizer composition according to claim 74, wherein said fertilizer composition is a seed coat, seed dressing, or seed treatment composition.

76. A fertilizer composition according to claim 74, wherein said fertilizer composition is a furrow application composition.

77. A fertilizer composition according to claim 74, wherein said fertilizer composition is provided to the crop during, before, or after planting.

78. A fertilizer composition according to claim 74, further comprising a porous carrier.

79. A fertilizer composition according to claim 74, additionally containing an additional synergistic component, which, when combined with said bacterial composition, increases the positive fertilizing effect of said fertilizer composition on the crop, which exceeds the total positive effect of its individual components.

80. A method of maintaining nitrogen levels in the soil, including:

planting in the soil of a field of an agricultural crop inoculated with genetically engineered bacteria that fix atmospheric nitrogen; And

harvesting said crop, wherein between planting and harvesting, no more than 90% of the dose of nitrogen required to produce said crop is applied to said soil of said field.

81. The method according to claim 80, where between planting and harvesting, no more than 80% of the nitrogen dose required to obtain said crop is applied to said soil of said field.

82. The method according to claim 80, where between planting and harvesting, no more than 70% of the nitrogen dose required to obtain said crop is applied to said soil of said field.

83. The method according to claim 80, where between planting and harvesting, no more than 60% of the nitrogen dose required to obtain said agricultural crop is applied to the specified soil of the specified field.

84. The method according to claim 80, where between planting and harvesting, no more than 50% of the nitrogen dose required to obtain said agricultural crop is applied to the specified soil of the specified field.

85. The method according to claim 80, where between planting and harvesting, no more than 40% of the nitrogen dose required to obtain said crop is applied to said soil of said field.

86. The method according to p. 80, where the specified genetically engineered bacterium contains a bacterial composition according to any one of the preceding paragraphs.

87. The method according to p. 80, where the specified genetically engineered bacterium consists of a bacterial composition according to any of the preceding paragraphs.

88. A method for delivering a probiotic supplement to an agricultural crop, including:

coating the seeds of the crop with a seed coating, seed dressing, or seed dressing, wherein said seed coat, seed dressing, or seed dressing contains live representatives of said probiotic; And

introduction into the soil of the field of the specified seeds of an agricultural crop.

89. The method of claim 88, wherein said seed coat, seed dressing, or seed treatment is applied to said crop seed in a single layer.

90. The method of claim 88, wherein said seed coating is applied to said crop seeds in multiple layers.

91. The method of claim 88, wherein said seed coating is applied to said crop seeds in a mixture.

92. The method of claim 88, wherein said crop seeds are non-nodulating.

93. The method according to p. 88, where the specified seed coating contains a bacterial composition according to any one of the preceding paragraphs.

94. The method according to any one of the preceding claims, where the genetically engineered bacterial strain is a genetically engineered strain of a Gram-positive bacterium.

95. The method of claim 94, wherein the genetically engineered Gram-positive bacterium has an altered expression level of the Nif cluster regulator.

96. The method of claim 94, wherein the genetically engineered Gram-positive bacterium expresses a reduced amount of a negative Nif cassette regulator.

97. The method of claim 94, wherein the genetically engineered bacterial strain expresses a reduced amount of GlnR .

98. The method according to any one of the preceding claims, wherein the genome of the genetically engineered bacterial strain encodes a polypeptide at least 75% identical to the sequence from the NifH database of the laboratory of J. Zehr.

99. The method according to any one of the preceding claims, wherein the genome of the genetically engineered bacterial strain encodes a polypeptide that is at least 85% identical to the sequence from the NifH database of the laboratory of J. Zehr.

100. The method according to any one of the preceding claims, wherein the genome of the genetically engineered bacterial strain encodes a polypeptide that is at least 75% identical to the sequence from the NifH database of D. Buckley's laboratory.

101. The method according to any one of the preceding claims, wherein the genome of the genetically engineered bacterial strain encodes a polypeptide that is at least 85% identical to the sequence from the NifH database of D. Buckley's laboratory.

102. A method for breeding strains of microorganisms to improve specific signs of agrotechnical significance, including:

providing a plurality of strains of microorganisms having the ability to colonize the desired crop;

improvement of regulatory networks affecting the trait through intragenomic rearrangement;

evaluating strains of microorganisms in a plurality of strains of microorganisms to determine the measure of the sign; And

selection from a plurality of strains of microorganisms of one or more strains of microorganisms, which provide an improvement in the trait in the case of the desired crop.

103. The method of claim 102, wherein the particular trait to be improved is the ability of the microorganism strain to fix nitrogen.

104. The method of claim 103, wherein the particular trait to be improved is the ability of the microorganism strain to fix atmospheric nitrogen in the presence of N-fertilize growth conditions.

105. A method for breeding strains of microorganisms to improve specific signs of agrotechnical significance, including:

providing a plurality of strains of microorganisms having the ability to colonize the desired crop;

the introduction of genetic diversity in many strains of microorganisms;

evaluating strains of microorganisms in a plurality of strains of microorganisms to determine the measure of the sign; And

selection from a plurality of strains of microorganisms of one or more strains of microorganisms, which provide an improvement in the trait in the case of the desired crop.

106. The method of claim 105, wherein the particular trait to be improved is the ability of the microorganism strain to fix nitrogen.

107. The method of claim 106 wherein the particular trait to be improved is the ability of the microorganism strain to fix atmospheric nitrogen in the presence of N-fertilize growth conditions.

108. A method for increasing the amount of nitrogen obtained from the atmosphere in a non-legume plant, including:

exposing said non-legume plant to genetically engineered non-intergeneric microorganisms, wherein said genetically engineered non-intergeneric microorganisms carry at least one genetic variation introduced into the nitrogen fixation genetic regulatory network, or at least one genetic variation introduced into the genetic regulatory nitrogen assimilation network.

109. The method of claim 108, wherein said genetically engineered non-intergeneric microorganisms carry at least one genetic variation introduced into said nitrogen fixation genetic regulatory network.

110. The method of claim 108, wherein said genetically engineered non-intergeneric microorganisms carry at least one genetic variation introduced into said nitrogen assimilation genetic regulatory network.

111. The method of claim 108, wherein said genetically engineered non-intergeneric microorganisms carry at least one genetic variation introduced into said nitrogen fixation genetic regulatory network and at least one genetic variation introduced into said nitrogen assimilation genetic regulatory network .

112. The method of claim 108, wherein said genetically engineered, non-intergeneric microorganisms are introduced into furrows into which seeds of said non-legume are planted.

113. The method of claim 108, wherein said genetically engineered non-intergeneric microorganisms are applied to the seeds of said non-legume plant.

114. The method of claim 108, wherein said non-legume is a non-legume crop selected from the group consisting of sorghum, canola, tomato, strawberry, barley, rice, corn, wheat, potato, millet, cereal, cereal, and corn.

115. The method of claim 108, wherein said genetically engineered non-intergeneric microorganisms colonize at least a root of said non-legume plant such that said genetically engineered non-intergeneric micro-organisms are present in said non-legume plant in an amount of at least 10 ⁵ colony forming units per gram wet weight of tissue.

116. The method of claim 108, wherein said genetically engineered non-intergeneric microorganisms are capable of fixing atmospheric nitrogen under non-nitrogen-limiting conditions.

117. The method of claim 108, wherein said genetically engineered, non-intergeneric microorganisms, in planta , excrete nitrogen-containing nitrogen fixation products.

118. The method of claim 108, wherein said at least one genetic variation is introduced into a gene selected from the group consisting of nifA, nifL, ntrB, ntrC , polynucleotide encoding glutamine synthetase, glnA, glnB, glnK, drat, amtB , polynucleotide , encoding glutaminase, glnD, glnE, nifJ, nifH, nifD, nifK, nifY, nifE, nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, nifQ, and the gene associated with the biosynthesis of the nitrogenase enzyme.

119. The method of claim 108, wherein said genetically engineered non-intergeneric microorganisms, in planta , produce at least 1% fixed nitrogen in said non-legume plant.

120. The method of claim 119, wherein said fixed nitrogen in said non-legume plant produced by said genetically engineered non-intergeneric microorganisms is determined by diluting ¹⁵ N in crops grown in fields treated with a fertilizer containing 1.2% ¹⁵ N .

121. The method of claim 119, wherein said genetically engineered non-intergeneric microorganisms, in planta , produce 5% or more fixed nitrogen in said non-legume plant.

122. The method of claim 108, wherein said non-intergeneric microorganisms are genetically engineered using at least one type of engineering selected from the group consisting of site-directed mutagenesis, random mutagenesis, and site-directed evolution.

123. A method for increasing atmospheric nitrogen in a corn plant, comprising

exposing said corn plant to genetically engineered non-intergeneric microorganisms carrying genetically engineered genetic variations in at least two genes selected from the group consisting of nifL , glnB , glnE and amtB .

124. The method of claim 123, wherein said genetically engineered, non-intergeneric microorganisms, in planta , excrete nitrogen-containing nitrogen fixation products.

125. The method of claim 123, wherein said genetically engineered, non-intergeneric microorganisms are applied to furrows in which seeds of said corn plant are planted.

126. The method of claim 123, wherein said genetically engineered, non-intergeneric microorganisms are applied to seeds of said corn plant.

127. A method for increasing the amount of nitrogen obtained from the atmosphere in a corn plant, including:

exposure of said corn plant to genetically engineered non-intergeneric microorganisms carrying at least one genetic variation introduced into the nitrogen fixation genetic regulatory network and at least one genetic variation introduced into the nitrogen assimilation genetic regulatory network, where said genetically engineered non-intergeneric microorganisms, in planta , produce at least 5% fixed nitrogen in said corn plant, as measured by ¹⁵ N dilution in crops grown in fields treated with a fertilizer containing 1.2% ¹⁵ N.

128. A method for increasing nitrogen fixation in a non-legume plant, comprising:

a. applying to a plant a plurality of non-intergeneric bacteria, wherein said plurality includes non-intergeneric bacteria that:

i. have an average colonizing capacity per unit of plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue; And

ii. produce a fixed N in an amount of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour, and

where the product of (i) the average colonizing capacity per unit plant root tissue and (ii) the fixed N produced per bacterial cell per hour is at least about 2.5 x 10 ^-8 mmol N per gram wet weight of plant root tissue per hour, and

where a plurality of non-intergeneric bacteria, in planta , produce 1% or more of fixed nitrogen in the plant, and

wherein each member of a plurality of non-intergeneric bacteria carries at least one genetic variation introduced into at least one gene or non-coding polynucleotide of the genetic regulatory network for nitrogen fixation or assimilation such that the non-intergeneric bacteria become capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen .

129. The method of claim 128, wherein at least one genetic variation comprises an introduced control sequence operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

130. The method of claim 128, wherein at least one genetic variation contains a promoter operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

131. The method of claim 128, wherein at least one genetic variation comprises an inducible promoter operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

132. The method of claim 128, wherein the plurality of non-intergeneric bacteria do not contain a constitutive promoter operably linked to a gene for a nitrogen fixation or assimilation genetic regulatory network.

133. The method of claim 128, wherein the plurality of non-intergeneric bacteria do not contain a constitutive promoter operably linked to a gene in the nif gene cluster.

134. The method of claim 128, wherein a plurality of non-intergeneric bacteria, in planta , excrete nitrogen-containing nitrogen fixation products.

135. The method of claim 128, wherein the plurality of non-intergeneric bacteria applied to the plant does not stimulate increased uptake of exogenous non-atmospheric nitrogen.

136. The method of claim 128, wherein the plant is grown in field soil to which at least about 22.7 kg of nitrogen-containing fertilizer per acre has been added, and wherein the nitrogen-containing fertilizer contains at least about 5% nitrogen by weight.

137. The method of claim 128, wherein the plant is grown in field soil to which at least about 22.7 kg of nitrogen-containing fertilizer per acre has been added, and where the nitrogen-containing fertilizer contains at least about 5% nitrogen by mass, and wherein the nitrogen-containing fertilizer contains ammonium or an ammonium-containing molecule.

138. The method of claim 128, wherein the exogenous nitrogen is selected from a fertilizer containing one or more of: glutamine, ammonia, ammonium, urea, nitrate, nitrite, ammonium-containing molecules, nitrate-containing molecules, and nitrite-containing molecules.

139. The method of claim 128, wherein the plurality of non-intergeneric bacteria, in planta , produce 5% or more fixed nitrogen in the plant.

140. The method of claim 128, wherein the plurality of non-intergeneric bacteria, in planta , produce 10% or more fixed nitrogen in the plant.

141. The method of claim 128, wherein the plurality of non-intergeneric bacteria, in planta , fixes atmospheric nitrogen under non-nitrogen-limiting conditions.

142. The method of claim 128, wherein a plurality of non-intergeneric bacteria, in planta , excrete nitrogen-containing nitrogen fixation products.

143. The method of claim 128, wherein the fixed nitrogen produced by a plurality of non-intergeneric bacteria is determined by diluting the enriched fertilizer with atmospheric N ₂ gas in plant tissue.

144. The method according to p. 128, where at least one gene or non-coding polynucleotide of the genetic regulatory network of nitrogen fixation or assimilation is selected from the group consisting of: nifA, nifL, ntrB, ntrC , polynucleotide encoding glutamine synthetase, glnA, glnB, glnK, Drat, AMTB , polynucleotide, encoding glutamine, GLND, GLNE, NIFJ, NIFH, NIFD, NIFK, NIFY, NIFE, NIFN, NIFU, NIFS, NIFV, NIFW, NIFZ, NIFM, NIFF, NIFB, NIFQ and Gene associated With biosynthesis nitrogenase enzyme.

145. The method according to p. 128, where at least one genetic variation is a mutation that leads to one or more of: increased expression or activity of NifA or glutaminase; reduced expression or activity of nifL, NtrB, glutamine synthetase, GlnB, GlnK, DraT, AmtB; reduced GlnE adenylyl scavenging activity; or reduced GlnD uridylate scavenging activity.

146. The method according to p. 128, where at least one genetic variation is selected from: (A) a knockout mutation; (B) removing or eliminating the regulatory sequence of the target gene; (C) the presence of a heterologous regulatory sequence insertion or (D) a domain deletion.

147. The method of claim 128, wherein at least one genetic variation is a mutant nifL gene that is altered to contain a heterologous promoter inserted into said nifL gene.

148. The method of claim 128, wherein at least one genetic variation is a mutant glnE gene that results in a truncated glnE protein lacking an adenylyl-removing (AR) domain.

149. The method of claim 128, wherein at least one genetic variation is a mutant amtB gene that results in a lack of expression of said amtB gene.

150. The method according to p. 128, where at least one genetic variation is selected from: a mutant nifL gene that is modified to contain a heterologous promoter built into the specified nifL gene; a mutant glnE gene that results in a truncated glnE protein lacking an adenylyl-removal (AR) domain; a mutant amtB gene that results in no expression of said amtB gene; and their combinations.

151. The method according to p. 128, where at least one genetic variation is a mutant nifL gene, which is modified to contain a heterologous promoter inserted into the specified nifL gene, and a mutant glnE gene, which leads to the formation of a truncated glnE protein with no adenylyl-removing (AR) domain.

152. The method according to p. 128, where at least one genetic variation is a mutant nifL gene, which is modified to contain a heterologous promoter inserted into the specified nifL gene, and a mutant glnE gene, which leads to the formation of a truncated glnE protein with no an adenylyl-removing (AR) domain and a mutant amtB gene that results in the absence of said amtB gene expression.

153. The method according to item 128, where the plant contains seeds, stem, flower, fruit, leaves or rhizome.

154. The method of claim 128, wherein the plurality of non-intergeneric bacteria are formulated into a composition.

155. The method of claim 128, wherein the plurality of non-intergeneric bacteria are formulated into a composition containing an agriculturally acceptable carrier.

156. The method of claim 128, wherein a plurality of non-intergeneric bacteria are introduced into the furrows in which seeds of said plant are planted.

157. The method of claim 128, wherein the plurality of non-intergeneric bacteria is formulated into a furrow liquid composition containing the bacteria at a concentration of about 1 x 10 ⁷ to about 1 x 10 ¹⁰ cfu per milliliter.

158. The method of claim 128, wherein a plurality of non-intergeneric bacteria is applied to the seeds of said plant.

159. The method of claim 128, wherein the plurality of non-intergeneric bacteria are formulated into a seed coat and applied to the seeds of said plant.

160. The method of claim 128, wherein a plurality of non-intergeneric bacteria are formulated into a seed coat and applied to the seeds of said plant at a concentration of about 1 x 10 ⁵ to about 1 x 10 ⁷ CFU per seed.

161. The method of claim 128, wherein the plant is a cereal crop.

162. The method of claim 128, wherein the plant is selected from the group consisting of: corn, rice, wheat, barley, sorghum, millet, oats, rye, and triticale.

163. The method of claim 128, wherein the plant is corn.

164. The method of claim 128, wherein the plant is a crop.

165. The method of claim 128, wherein the plant is a genetically modified organism.

166. The method of claim 128, wherein the plant is not a genetically modified organism.

167. The method of claim 128 wherein the plant has been genetically engineered or bred to use nitrogen efficiently

168. The method of claim 128, wherein the plurality of non-intergeneric bacteria comprises at least two different species of bacteria.

169. The method of claim 128, wherein the plurality of non-intergeneric bacteria comprises at least two different strains of the same bacterial species.

170. The method of claim 128, wherein the plurality of non-intergeneric bacteria contains bacteria selected from: Rahnella aquatilis , Klebsiella variicola , Achromobacter spiritinus , Achromobacter marplatensis , Microbacterium murale , Kluyvera intermedia , Kosakonia pseudosacchari , Enterobacter species, Azospirillum lipoferum , Kosakonia sacchari and their combinations.

171. The method of claim 128, wherein the plurality of non-intergeneric bacteria are endophytic, epiphytic, or rhizospheric.

172. The method according to p. 128, where the set of non-intergeneric bacteria contains bacteria selected from: bacteria deposited as PTA-122293, bacteria deposited as PTA-122294, bacteria deposited as NCMA 201701002, bacteria deposited as NCMA 201708004, bacteria deposited as NCMA 201708003, bacteria deposited as NCMA 201708002, bacteria deposited as NCMA 201712001, bacteria deposited as NCMA 201712002, and combinations thereof.

173. A method for increasing nitrogen fixation in a non-legume plant, including:

a. applying a plurality of bacteria to a plant, wherein said plurality includes bacteria that:

i. have an average colonizing capacity per unit of plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue; and/or

ii. produce a fixed N in an amount of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour, and

where a plurality of bacteria, in planta , produce 1% or more fixed nitrogen in the plant.

174. The method of claim 173, wherein the plurality of bacteria comprises bacteria that: have an average colonizing capacity per unit of plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue.

175. The method of claim 173, wherein the plurality of bacteria comprise bacteria that: produce a fixed N of at least about 173 x 10 ^-17 mmol N per bacterial cell per hour.

176. The method of claim 173, wherein the plurality of bacteria comprises bacteria that: have an average colonizing capacity per unit plant root tissue of at least about 1.0 × 10 ⁴ bacterial cells per gram wet weight of plant root tissue and produce a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour.

177. The method of claim 173, wherein the plurality of bacteria comprises bacteria that: have an average colonizing capacity per unit of plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue and produce a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour, and where the product of (i) the average colonizing capacity per unit of plant root tissue and (ii) the fixed N produced per bacterial cell per hour is at least about 2, 5 x 10 ^-8 mmol N per gram wet weight of plant root tissue per hour.

178. The method of claim 173, wherein a plurality of bacteria, in planta , excrete nitrogen-containing nitrogen fixation products.

179. The method according to p. 173, where a lot of bacteria applied to the plant does not stimulate an increase in the uptake of exogenous non-atmospheric nitrogen.

180. The method according to item 173, where the plant contains seeds, stem, flower, fruit, leaves or rhizome.

181. The method of claim 173, wherein the plurality of bacteria are formulated into a composition.

182. The method of claim 173, wherein the plurality of bacteria are formulated into a composition containing an agriculturally acceptable carrier.

183. The method according to p. 173, where a lot of bacteria are brought into the furrows in which the seeds of the specified plant are planted.

184. The method of claim 173, wherein the plurality of bacteria are formulated into a liquid furrow application containing bacteria at a concentration of from about 1 x 10 ⁷ to about 1 x 10 ¹⁰ CFU per milliliter.

185. The method according to p. 173, where a lot of bacteria are applied to the seeds of the specified plant.

186. The method of claim 173, wherein the plurality of bacteria are formulated into a seed coat and applied to the seeds of said plant.

187. The method of claim 173, wherein the plurality of bacteria are formulated into a seed coat and applied to the seeds of said plant at a concentration of about 1 x 10 ⁵ to about 1 x 10 ⁷ cfu per seed.

188. The method of claim 173, wherein the plant is a cereal crop.

189. The method of claim 173, wherein the plant is selected from the group consisting of: corn, rice, wheat, barley, sorghum, millet, oats, rye, and triticale.

190. The method of claim 173, wherein the plant is corn.

191. The method of claim 173, wherein the plant is a crop.

192. The method of claim 173, wherein the plant is a genetically modified organism.

193. The method of claim 173, wherein the plant is not a genetically modified organism.

194. The method of claim 173 wherein the plant has been genetically engineered or bred to use nitrogen efficiently

195. The method according to p. 173, where the set of bacteria contains at least two different types of bacteria.

196. The method of claim 173, wherein the plurality of bacteria contains at least two different strains of the same bacterial species.

197. The method of claim 173, wherein the plurality of bacteria contains bacteria selected from: Rahnella aquatilis , Klebsiella variicola , Achromobacter spiritinus , Achromobacter marplatensis , Microbacterium murale , Kluyvera intermedia , Kosakonia pseudosacchari , Enterobacter species, Azospirillum lipoferum , Kosakonia s acchari and their combinations.

198. The method of claim 173, wherein the plurality of bacteria are endophytic, epiphytic, or rhizospheric.

199. The method of claim 173, wherein the plurality of bacteria are selected from: bacteria deposited as NCMA 201701003, bacteria deposited as NCMA 201701001, and bacteria deposited as NCMA 201708001.

200. A non-intergeneric bacterial population capable of increasing nitrogen fixation in a non-legume plant, comprising:

a. a variety of non-intergeneric bacteria that:

i. have an average colonizing capacity per unit of plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue; and/or

ii. produce a fixed N in an amount of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour, and

where the plurality of non-intergeneric bacteria, in planta , produce 1% or more fixed nitrogen in the plant, grown in the presence of the plurality of non-intergeneric bacteria, and

wherein each member of a plurality of non-intergeneric bacteria carries at least one genetic variation introduced into at least one gene or non-coding polynucleotide of the genetic regulatory network for nitrogen fixation or assimilation such that the non-intergeneric bacteria become capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen .

201. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria comprises bacteria that: have an average colonizing capacity per unit of plant root tissue of at least about 1.0 × 10 ⁴ bacterial cells per gram wet weight of root tissue plants.

202. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria comprises bacteria that: produce a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour.

203. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria comprises bacteria that: have an average colonizing capacity per unit of plant root tissue of at least about 1.0 × 10 ⁴ bacterial cells per gram wet weight of root tissue plants and produce a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour.

204. A non-intergeneric population of bacteria according to claim 200, wherein at least one genetic variation contains an introduced control sequence operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

205. A non-intergeneric population of bacteria according to claim 200, wherein at least one genetic variation contains a promoter operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

206. A non-intergeneric population of bacteria according to claim 200, wherein at least one genetic variation contains an inducible promoter operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

207. A non-intergeneric population of bacteria according to claim 200, wherein the plurality of non-intergeneric bacteria does not contain a constitutive promoter operably linked to a gene for nitrogen fixation or assimilation genetic regulatory network.

208. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria does not contain a constitutive promoter operably linked to a gene in the nif gene cluster.

209. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria, in planta , excrete nitrogen-containing nitrogen fixation products.

210. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria applied to the plant does not stimulate increased uptake of exogenous non-atmospheric nitrogen.

211. The non-intergeneric bacterial population of claim 200, wherein the plant is grown in field soil to which at least about 22.7 kg of nitrogen-containing fertilizer per acre has been added, and where the nitrogen-containing fertilizer contains at least about 5% nitrogen by weight.

212. The non-intergeneric bacterial population of claim 200, wherein the plant is grown in field soil to which at least about 22.7 kg of nitrogen-containing fertilizer per acre has been added, and where the nitrogen-containing fertilizer contains at least about 5% nitrogen by weight, and wherein the nitrogen-containing fertilizer contains ammonium or an ammonium-containing molecule.

213. The non-intergeneric bacterial population of claim 200, wherein the exogenous nitrogen is selected from a fertilizer containing one or more of: glutamine, ammonia, ammonium, urea, nitrate, nitrite, ammonium-containing molecules, nitrate-containing molecules, and nitrite-containing molecules.

214. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria, in planta , produces 5% or more of fixed nitrogen in the plant.

215. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria, in planta , produces 10% or more of fixed nitrogen in the plant.

216. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria, in planta , fixes atmospheric nitrogen under nitrogen-limiting conditions.

217. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria, in planta , excrete nitrogen-containing nitrogen fixation products.

218. The non-intergeneric bacterial population of claim 200, wherein the fixed nitrogen produced by the plurality of non-intergeneric bacteria is determined by diluting the enriched fertilizer with atmospheric N ₂ gas in plant tissue.

219. A non-intergeneric population of bacteria according to claim 200, where at least one gene or non-coding polynucleotide of the genetic regulatory network of nitrogen fixation or assimilation is selected from the group consisting of: nifA, nifL, ntrB, ntrC , a polynucleotide encoding glutamine synthetase, glnA, glnB, glnK, drat, amtB , a polynucleotide encoding glutaminase, glnD, glnE, nifJ, nifH, nifD, nifK, nifY, nifE, nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, nifQ, and gene associated with the biosynthesis of the nitrogenase enzyme.

220. Non-intergeneric population of bacteria according to item 200, where at least one genetic variation is a mutation that leads to one or more of: increased expression or activity of NifA or glutaminase; reduced expression or activity of nifL, NtrB, glutamine synthetase, GlnB, GlnK, DraT, AmtB; reduced GlnE adenylyl scavenging activity; or reduced GlnD uridylate scavenging activity.

221. Non-intergeneric population of bacteria according to item 200, where at least one genetic variation is selected from: (A) a knockout mutation; (B) removing or eliminating the regulatory sequence of the target gene; (C) the presence of a heterologous regulatory sequence insertion or (D) a domain deletion.

222. A non-intergeneric population of bacteria according to claim 200, wherein at least one genetic variation is a mutant nifL gene that is altered to contain a heterologous promoter inserted into said nifL gene.

223. The non-intergeneric bacterial population of claim 200, wherein at least one genetic variation is a mutant glnE gene that results in a truncated glnE protein lacking an adenylyl-removing (AR) domain.

224. The non-intergeneric bacterial population of claim 200, wherein at least one genetic variation is a mutant amtB gene that results in a lack of expression of said amtB gene.

225. A non-intergeneric population of bacteria according to claim 200, where at least one genetic variation is selected from: a mutant nifL gene that is modified to contain a heterologous promoter inserted into said nifL gene; a mutant glnE gene that results in a truncated glnE protein lacking an adenylyl-removing (AR) domain; a mutant amtB gene that results in no expression of said amtB gene; and their combinations.

226. A non-intergeneric population of bacteria according to claim 200, where at least one genetic variation is a mutant nifL gene that is modified to contain a heterologous promoter inserted into said nifL gene and a mutant glnE gene that results in the formation of a truncated protein glnE lacking an adenylyl-removal (AR) domain.

227. A non-intergeneric population of bacteria according to claim 200, where at least one genetic variation is a mutant nifL gene that is modified to contain a heterologous promoter inserted into said nifL gene and a mutant glnE gene that results in the formation of a truncated protein glnE lacking an adenylyl-removal (AR) domain and a mutant amtB gene that results in the absence of said amtB gene expression.

228. A non-intergeneric population of bacteria according to claim 200, where the plant contains seeds, stem, flower, fruit, leaves or rhizome.

229. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria are formulated into a composition.

230. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria is formulated into a composition containing an agriculturally acceptable carrier.

231. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria is formulated into a liquid furrow composition containing the bacteria at a concentration of about 1 x 10 ⁷ to about 1 x 10 ¹⁰ CFU per milliliter.

232. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria is formulated into a seed coating.

233. The non-intergeneric bacterial population of claim 200, wherein the plant is a cereal crop.

234. A non-intergeneric population of bacteria according to claim 200, where the plant is selected from the group consisting of: corn, rice, wheat, barley, sorghum, millet, oats, rye and triticale.

235. The non-intergeneric bacterial population of claim 200, wherein the plant is corn.

236. The non-intergeneric bacterial population of claim 200, wherein the plant is a crop.

237. A non-intergeneric population of bacteria according to claim 200, where the plant is a genetically modified organism.

238. A non-intergeneric population of bacteria according to claim 200, where the plant is not a genetically modified organism.

239. A non-intergeneric population of bacteria according to claim 200, where the plant has been genetically engineered or bred to use nitrogen efficiently

240. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria contains at least two different species of bacteria.

241. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria contains at least two different strains of the same bacterial species.

242. A non-intergeneric population of bacteria according to claim 200, where the plurality of non-intergeneric bacteria contains bacteria selected from: Rahnella aquatilis , Klebsiella variicola , Achromobacter spiritinus , Achromobacter marplatensis , Microbacterium murale , Kluyvera intermedia , Kosakonia pseudosacchari , species Enterobacter , Azospirill um lipoferum , Kosakonia sacchari and their combinations.

243. The non-intergeneric bacterial population of claim 200, wherein the plurality of non-intergeneric bacteria are endophytic, epiphytic, or rhizospheric.

244. A non-intergeneric population of bacteria according to claim 200, where the plurality of non-intergeneric bacteria contains bacteria selected from: bacteria deposited as PTA-122293, bacteria deposited as PTA-122294, bacteria deposited as NCMA 201701002, bacteria deposited as NCMA 201708004, bacteria deposited as NCMA 201708003, bacteria deposited as NCMA 201708002, bacteria deposited as NCMA 201712001, bacteria deposited as NCMA 201712002, and combinations thereof.

245. A population of bacteria capable of increasing nitrogen fixation in a non-legume plant, containing:

a. many bacteria that

i. have an average colonizing capacity per unit of plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue; and/or

ii. produce a fixed N in an amount of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour, and

where a plurality of bacteria, in planta , produce 1% or more fixed nitrogen in the plant.

246. The bacterial population of claim 245, wherein the plurality of bacteria comprises bacteria that: have an average colonizing capacity per unit of plant root tissue of at least about 1.0 × 10 ⁴ bacterial cells per gram wet weight of plant root tissue.

247. The bacterial population of claim 245, wherein the plurality of bacteria comprises bacteria that: produce a fixed N of at least about 245 x 10 ^-17 mmol N per bacterial cell per hour.

248. The bacterial population of claim 245, wherein the plurality of bacteria comprises bacteria that: have an average colonizing capacity per unit of plant root tissue of at least about 1.0 × 10 ⁴ bacterial cells per gram wet weight of plant root tissue and produce a fixed N in an amount of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour.

249. The bacterial population of claim 245, wherein the plurality of bacteria comprises bacteria that: have an average colonizing capacity per unit of plant root tissue of at least about 1.0 × 10 ⁴ bacterial cells per gram wet weight of plant root tissue and produce a fixed N in an amount of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour, and where the product of (i) the average colonizing capacity per unit of plant root tissue and (ii) the fixed N produced per bacterial cell per hour is at least about 2 .5 × 10 ^-8 mmol N per gram wet weight of plant root tissue per hour.

250. The bacterial population of claim 245, wherein the plurality of bacteria, in planta , excrete nitrogen-containing nitrogen fixation products.

251. The bacterial population of claim 245, wherein the plurality of bacteria applied to the plant does not stimulate increased uptake of exogenous non-atmospheric nitrogen.

252. The bacterial population of claim 245, wherein the plant contains seeds, stem, flower, fruit, leaves, or rhizome.

253. The bacterial population of claim 245, wherein the plurality of bacteria are formulated into a composition.

254. The bacterial population of claim 245, wherein the plurality of bacteria are formulated into a composition containing an agriculturally acceptable carrier.

255. A population of bacteria according to claim 245, where a plurality of bacteria are introduced into the furrows in which the seeds of the specified plant are planted.

256. The bacterial population of claim 245, wherein the plurality of bacteria is formulated into a liquid furrow application containing bacteria at a concentration of about 1 x 10 ⁷ to about 1 x 10 ¹⁰ cfu per milliliter.

257. A population of bacteria according to claim 245, wherein a plurality of bacteria is applied to the seeds of said plant.

258. The bacterial population of claim 245, wherein the plurality of bacteria is formulated into a seed coating and applied to the seeds of said plant.

259. The bacterial population of claim 245, wherein the plurality of bacteria are formulated into a seed coat and applied to the seeds of said plant at a concentration of about 1 x 10 ⁵ to about 1 x 10 ⁷ CFU per seed.

260. The bacterial population of claim 245, wherein the plant is a cereal crop.

261. The bacterial population of claim 245, wherein the plant is selected from the group consisting of: corn, rice, wheat, barley, sorghum, millet, oats, rye, and triticale.

262. The bacterial population of claim 245, wherein the plant is corn.

263. The bacterial population of claim 245, wherein the plant is a crop.

264. The bacterial population of claim 245, where the plant is a genetically modified organism.

265. A population of bacteria according to clause 245, where the plant is not a genetically modified organism.

266. Population of bacteria according to clause 245, where the plant has been genetically engineered or bred to use nitrogen efficiently

267. The bacterial population of claim 245, wherein the plurality of bacteria contains at least two different bacterial species.

268. The bacterial population of claim 245, wherein the plurality of bacteria contains at least two different strains of the same bacterial species.

269. A population of bacteria according to claim 245, where a plurality of bacteria contains bacteria selected from: Rahnella aquatilis , Klebsiella variicola , Achromobacter spiritinus , Achromobacter marplatensis , Microbacterium murale , Kluyvera intermedia , Kosakonia pseudosacchari , Enterobacter species, Azospirillum lipoferum , Kosak onia sacchari and their combinations .

270. The bacterial population of claim 245, wherein the plurality of bacteria are endophytic, epiphytic, or rhizospheric.

271. The bacterial population of claim 245, wherein the plurality of bacteria are selected from: bacteria deposited as NCMA 201701003, bacteria deposited as NCMA 201701001, and bacteria deposited as NCMA 201708001.

272. A bacterium that:

i. has an average colonizing capacity per unit of plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue; and/or

ii. produces a fixed N in an amount of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour.

273. A non-intergeneric bacterium carrying: at least one genetic variation introduced into at least one gene or non-coding polynucleotide of the nitrogen fixation or assimilation genetic regulatory network so that the non-intergeneric bacterium is capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen, and where is the indicated bacterium:

i. has an average colonizing capacity per unit plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue; and/or

ii. produces a fixed N in an amount of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour.

274. The non-intergeneric bacterium of claim 273, wherein the bacterium has an average colonizing capacity per unit of plant root tissue of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue.

275. The non-intergeneric bacterium of claim 273, wherein the bacterium produces a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour.

276. The non-intergeneric bacterium of claim 273, wherein the bacterium has an average colonizing capacity per unit plant root tissue of at least about 1.0 × 10 ⁴ bacterial cells per gram wet weight of plant root tissue and produces a fixed N of at least approximately 1 x 10 ^-17 mmol N per bacterial cell per hour.

277. The non-intergeneric bacterium of claim 273, wherein the bacterium has an average colonizing capacity per unit plant root tissue of at least about 1.0 × 10 ⁴ bacterial cells per gram wet weight of plant root tissue and produces a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour, and where the product of (i) the average colonizing capacity per unit of plant root tissue and (ii) the fixed N produced per bacterial cell per hour is at least about 2.5 x 10 ^-8 mmol N per gram wet weight of plant root tissue per hour.

278. A non-intergeneric bacterium according to claim 273, wherein at least one genetic variation contains an introduced control sequence operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

279. A non-intergeneric bacterium according to claim 273, wherein at least one genetic variation contains a promoter operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

280. A non-intergeneric bacterium according to claim 273, wherein at least one genetic variation contains an inducible promoter operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

281. A non-intergeneric bacterium according to claim 273, wherein the bacterium does not contain a constitutive promoter operably linked to the gene for the nitrogen fixation or assimilation genetic regulatory network.

282. The non-intergeneric bacterium of claim 273, wherein the bacterium does not contain a constitutive promoter operably linked to a gene in the nif gene cluster.

283. The non-intergeneric bacterium of claim 273, wherein the bacterium, in planta , excretes nitrogen-containing nitrogen fixation products.

284. A non-intergeneric bacterium according to claim 273, wherein at least one gene or non-coding polynucleotide of the nitrogen fixation or assimilation genetic regulatory network is selected from the group consisting of: nifA, nifL, ntrB, ntrC , a polynucleotide encoding glutamine synthetase, glnA, glnB , glnK, drat, amtB , полинуклеотида, кодирующего глутаминазу, glnD, glnE, nifJ, nifH, nifD, nifK, nifY, nifE, nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, nifQ и гена, associated with the biosynthesis of the nitrogenase enzyme.

285. Non-intergeneric bacterium according to item 273, where at least one genetic variation is a mutation that leads to one or more of: increased expression or activity of NifA or glutaminase; reduced expression or activity of nifL, NtrB, glutamine synthetase, GlnB, GlnK, DraT, AmtB; reduced GlnE adenylyl scavenging activity; or reduced GlnD uridylate scavenging activity.

286. Non-intergeneric bacterium according to item 273, where at least one genetic variation is selected from: (A) a knockout mutation; (B) removing or eliminating the regulatory sequence of the target gene; (C) the presence of a heterologous regulatory sequence insertion or (D) a domain deletion.

287. The non-intergeneric bacterium of claim 273, wherein at least one genetic variation is a mutant nifL gene that is altered to contain a heterologous promoter inserted into said nifL gene.

288. The non-intergeneric bacterium of claim 273, wherein at least one genetic variation is a mutant glnE gene that results in a truncated glnE protein lacking an adenylyl-removing (AR) domain.

289. The non-intergeneric bacterium of claim 273, wherein at least one genetic variation is a mutant amtB gene that results in a lack of expression of said amtB gene.

290. The non-intergeneric bacterium of claim 273, wherein at least one genetic variation is selected from: a mutant nifL gene that is modified to contain a heterologous promoter inserted into said nifL gene; a mutant glnE gene that results in a truncated glnE protein lacking an adenylyl-removal (AR) domain; a mutant amtB gene that results in no expression of said amtB gene; and their combinations.

291. The non-intergeneric bacterium of claim 273, wherein at least one genetic variation is a mutant nifL gene that is altered to contain a heterologous promoter inserted into said nifL gene and a mutant glnE gene that results in a truncated glnE protein with the absence of an adenylyl-removing (AR) domain.

292. The non-intergeneric bacterium of claim 273, wherein at least one genetic variation is a mutant nifL gene that is altered to contain a heterologous promoter inserted into said nifL gene and a mutant glnE gene that results in a truncated glnE protein with the absence of an adenylyl-removal (AR) domain and a mutant amtB gene that results in the absence of said amtB gene expression.

293. A non-intergeneric bacterium according to claim 273, formulated into a composition.

294. The non-intergeneric bacterium of claim 273, formulated into a composition containing an agriculturally acceptable carrier.

295. The non-intergeneric bacterium of claim 273, formulated into a liquid composition for application to the furrow.

296. The non-intergeneric bacterium of claim 273 formulated into a seed coating.

297. A non-intergeneric bacterium according to claim 273, where said bacterium is selected from: Rahnella aquatilis , Klebsiella variicola , Achromobacter spiritinus , Achromobacter marplatensis , Microbacterium murale , Kluyvera intermedia , Kosakonia pseudosacchari , Enterobacter species, Azospirillum lipoferum , Kosakonia sacchari and their combinations.

298. The non-intergeneric bacterium of claim 273, wherein said bacterium is endophytic, epiphytic, or rhizospheric.

299. The non-intergeneric bacterium of claim 273, wherein said bacterium is selected from: bacteria deposited as PTA-122293, bacteria deposited as PTA-122294, bacteria deposited as NCMA 201701002, bacteria deposited as NCMA 201708004, bacteria deposited as NCMA 201708003, bacteria deposited as NCMA 201708002, bacteria deposited as NCMA 201712001, bacteria deposited as NCMA 201712002, and combinations thereof.

300. A method for increasing nitrogen fixation in a plant, which includes introducing into the plant an effective amount of a composition containing:

i. a purified bacterial population that contains bacteria with a 16S nucleic acid sequence that is at least about 97% identical to a nucleic acid sequence selected from SEQ ID NOs: 85, 96, 111, 121, 122, 123, 124, 136, 149, 157, 167, 261, 262, 269, 277-283;

ii. a purified bacterial population that contains bacteria with a nucleic acid sequence that is at least about 90% identical to a nucleic acid sequence selected from SEQ ID NOs: 86-95, 97-110, 112-120, 125-135, 137-148 , 150-156, 158-166, 168-176, 263-268, 270-274, 275, 276, 284-295; and/or

iii. a purified bacterial population that contains bacteria with a nucleic acid sequence that is at least about 90% identical to a nucleic acid sequence selected from SEQ ID NOs: 177-260, 296-303; And

wherein a plant administered with an effective amount of the composition exhibits an increase in nitrogen fixation compared to a plant without administration of the composition.

301. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains bacteria with a 16S nucleic acid sequence that is at least about 97% identical to a nucleic acid sequence selected from SEQ ID NOs: 85, 96, 111, 121, 122, 123, 124, 136, 149, 157, 167, 261, 262, 269 and 277-283.

302. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains bacteria with a 16S nucleic acid sequence that is at least about 99% identical to a nucleic acid sequence selected from SEQ ID NOs: 85, 96, 111, 121, 122, 123, 124, 136, 149, 157, 167, 261, 262, 269 and 277-283.

303. The method according to p. 300, where the composition contains: a purified population of bacteria, which contains bacteria with a 16S nucleic acid sequence selected from SEQ ID NO: 85, 96, 111, 121, 122, 123, 124, 136, 149, 157 , 167, 261, 262, 269 and 277-283.

304. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains bacteria with a nucleic acid sequence that is at least about 90% identical to a nucleic acid sequence selected from SEQ ID NOs: 86-95, 97-110 , 112-120, 125-135, 137-148, 150-156, 158-166, 168-176, 263-268, 270-274, 275, 276 and 284-295

305. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains bacteria with a nucleic acid sequence that is at least about 95% identical to a nucleic acid sequence selected from SEQ ID NOs: 86-95, 97-110 , 112-120, 125-135, 137-148, 150-156, 158-166, 168-176, 263-268, 270-274, 275, 276 and 284-295

306. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains bacteria with a nucleic acid sequence that is at least about 99% identical to a nucleic acid sequence selected from SEQ ID NOs: 86-95, 97-110 , 112-120, 125-135, 137-148, 150-156, 158-166, 168-176, 263-268, 270-274, 275, 276 and 284-295

307. The method according to p. 300, where the composition contains: a purified population of bacteria, which contains bacteria with a nucleic acid sequence selected from SEQ ID NO: 86-95, 97-110, 112-120, 125-135, 137-148, 150-156, 158-166, 168-176, 263-268, 270-274, 275, 276 and 284-295.

308. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains bacteria with a nucleic acid sequence that is at least about 90% identical to a nucleic acid sequence selected from SEQ ID NOs: 177-260 and 296-303 .

309. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains bacteria with a nucleic acid sequence that is at least about 95% identical to a nucleic acid sequence selected from SEQ ID NOs: 177-260 and 296-303 .

310. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains bacteria with a nucleic acid sequence that is at least about 99% identical to a nucleic acid sequence selected from SEQ ID NOs: 177-260 and 296-303 .

311. The method according to p. 300, where the composition contains: a purified population of bacteria, which contains bacteria with a nucleic acid sequence selected from SEQ ID NO: 177-260 and 296-303.

312. The method according to p. 300, where the composition contains: a purified population of bacteria that contains bacteria with at least one genetic variation introduced into a gene selected from the group consisting of nifA , nifL , ntrB , ntrC , a polynucleotide encoding glutamine synthetase, glnA , glnB , glnK , drat, amtB , polynucleotide encoding glutaminase, glnD , glnE , nifJ , nifH , nifD , nifK , nifY , nifE , nifN , nifU , nifS , nifV , nifW , nifZ , nifM , nifF , nifB , nifQ and a gene associated with the biosynthesis of the nitrogenase enzyme.

313. The method according to p. 300, where the composition contains: a purified population of bacteria that contains bacteria with at least one genetic variation introduced into a gene selected from the group consisting of nifA , nifL , ntrB , ntrC , a polynucleotide encoding glutamine synthetase, glnA , glnB , glnK , drat, amtB , polynucleotide encoding glutaminase, glnD , glnE , nifJ , nifH , nifD , nifK , nifY , nifE , nifN , nifU , nifS , nifV , nifW , nifZ , nifM , nifF , nifB , nifQ and a gene associated with the biosynthesis of nitrogenase enzyme, where the genetic variation (A) is a knockout mutation; (B) removes or eliminates the regulatory sequence of the target gene; (C) contains an insert of a heterologous regulatory sequence or (D) is a domain deletion.

314. The method according to p. 300, where the composition contains: a purified population of bacteria that contains bacteria with at least one mutation that leads to one or more of: increased expression or activity of NifA or glutaminase; decreased expression or activity of nifL, NtrB, glutamine synthetase, GlnB, GlnK, DraT, or AmtB; reduced GlnE adenylyl scavenging activity; or reduced GlnD uridylate scavenging activity.

315. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains bacteria with a mutated nifL gene that is altered to contain a heterologous promoter inserted into said nifL gene.

316. The method of claim 300 wherein the composition comprises: a purified bacterial population that contains bacteria with a mutant glnE gene that results in a truncated glnE protein lacking an adenylyl-removing (AR) domain.

317. The method according to p. 300, where the composition contains: a purified population of bacteria that contains bacteria with a mutant amtB gene, which leads to a lack of expression of the specified amtB gene.

318. The method according to p. 300, where the composition contains: a purified population of bacteria that contains bacteria with at least one of the following genetic alterations: a mutant nifL gene that is modified to contain a heterologous promoter inserted into the specified nifL gene; a mutant glnE gene that results in a truncated glnE protein lacking an adenylyl-removal (AR) domain; a mutant amtB gene that results in no expression of said amtB gene; and their combinations.

319. The method according to p. 300, where the composition contains: a purified population of bacteria that contains bacteria with a mutant nifL gene that is modified to contain a heterologous promoter built into the specified nifL gene and a mutant glnE gene that leads to the formation of a truncated glnE protein with the absence of an adenylyl-removing (AR) domain.

320. The method according to p. 300, where the composition contains: a purified population of bacteria that contains bacteria with a mutant nifL gene that is modified to contain a heterologous promoter built into the specified nifL gene and a mutant glnE gene that leads to the formation of a truncated glnE protein with the absence of an adenylyl-removal (AR) domain and a mutant amtB gene that results in the absence of said amtB gene expression.

321. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains nifL modified bacteria that express the NifL protein in less than about 50% of non- nifL modified bacteria.

322. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains nifL modified bacteria that express the NifL protein in less than about 50% of non- nifL modified bacteria, where the modification is disruption, knockout, or truncation.

323. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains nifL-modified bacteria that express the NifL protein in less than about 50% of non- nifL- modified bacteria, and wherein the bacteria further comprise a promoter operably linked to the nifA sequence .

324. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains nifL modified bacteria that express the NifL protein in less than about 50% of the non- nifL modified bacteria, and wherein the bacteria lacks the nifL homologue.

325. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains glnE modified bacteria that express glnE protein in less than about 50% of non- glnE modified bacteria.

326. The method of claim 300, wherein the composition comprises: a purified population of bacteria that contains glnE modified bacteria that express the glnE protein of less than about 50% of non- glnE modified bacteria, where the modification is disruption, knockout, or shortening.

327. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains glnE modified bacteria that express the glnE protein in less than about 50% of the glnE modified bacteria, and where the glnE protein sequence lacks an adenylyl-deleting (AR ) domain.

328. The method of claim 300, wherein the composition comprises: a purified bacterial population that contains glnE modified bacteria that express the glnE protein in less than about 50% of non -glnE modified bacteria, and where the glnE protein sequence lacks an adenylyl-removing (AR ) activity.

329. The method of claim 300, wherein the purified bacterial population, in planta , provides the plant with at least 1% fixed nitrogen.

330. The method of claim 300, wherein the purified bacterial population, in planta , provides the plant with at least 5% fixed nitrogen.

331. The method of claim 300, wherein the purified bacterial population, in planta , provides the plant with at least 10% fixed nitrogen.

332. The method of claim 300, wherein the purified bacterial population is capable of fixing atmospheric nitrogen under non-nitrogen-limiting conditions.

333. The method of claim 300, wherein the purified bacterial population, in planta , excretes nitrogen-containing nitrogen fixation products.

334. The method of claim 300, wherein the purified bacterial population, in planta , provides the plant with at least 1% fixed nitrogen, and wherein said fixed nitrogen is measured by diluting the enriched fertilizer with atmospheric N ₂ gas in plant tissue.

335. The method of claim 300, wherein the purified bacterial population colonizes the root of said plant and is present in an amount of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue.

336. The method according to item 300, where the plant contains seeds, stem, flower, fruit, leaves or rhizome.

337. The method of claim 300, wherein the composition contains: an agriculturally acceptable carrier.

338. The method of claim 300, wherein the composition containing the purified population of bacteria is applied to the furrows in which seeds of said plant are planted.

339. The method of claim 300, wherein the composition containing the purified population of bacteria is formulated as a liquid furrow composition containing bacteria at a concentration of from about 1 x 10 ⁷ to about 1 x 10 ¹⁰ CFU per milliliter.

340. The method according to p. 300, where the composition containing the purified population of bacteria is applied to the seeds of the specified plant.

341. The method of claim 300 wherein the composition comprising the purified bacterial population is formulated as a seed coat and applied to the seeds of said plant.

342. The method of claim 300, wherein the composition comprising the purified bacterial population is formulated as a seed coat and applied to the seeds of said plant at a concentration of about 1 x 10 ⁵ to about 1 x 10 ⁷ CFU per seed.

343. The method of claim 300, wherein the plant is a non-legume.

344. The method of claim 300, wherein the plant is a cereal crop.

345. The method of claim 300, wherein the plant is selected from the group consisting of: corn, rice, wheat, barley, sorghum, millet, oats, rye, and triticale.

346. The method of claim 300 wherein the plant is corn.

347. The method of claim 300, wherein the plant is a legume.

348. The method of claim 300, wherein the plant is a cereal crop.

349. The method of claim 300, wherein the purified bacterial population contains bacteria selected from: Rahnella aquatilis , Klebsiella variicola , Achromobacter spiritinus , Achromobacter marplatensis , Microbacterium murale , Kluyvera intermedia , Kosakonia pseudosacchari , Enterobacter species, Azospirillum lipoferum , Kosak onia sacchari and their combinations .

350. The method of claim 300 wherein the purified bacterial population contains bacteria of the genus Rahnella .

351. The method of claim 300 wherein the purified bacterial population contains bacteria of the species Rahnella aquatilis .

352. The method of claim 300, wherein the purified bacterial population contains bacteria deposited as PTA-122293.

353. The method of claim 300, wherein the purified bacterial population contains bacteria deposited as NCMA 201701003.

354. The method of claim 300, wherein the purified bacterial population contains bacteria of the genus Kosakonia .

355. The method of claim 300, wherein the purified bacterial population contains bacteria of the species Kosakonia sacchari .

356. The method of claim 300 wherein the purified bacterial population contains bacteria deposited as PTA-122294.

357. The method of claim 300, wherein the purified bacterial population contains bacteria deposited as NCMA 201701001.

358. The method of claim 300, wherein the purified bacterial population contains bacteria deposited as NCMA 201701002.

359. The method of claim 300, wherein the purified bacterial population contains bacteria deposited as NCMA 201708004.

360. The method of claim 300, wherein the purified bacterial population contains bacteria deposited as NCMA 201708003.

361. The method of claim 300, wherein the purified bacterial population contains bacteria deposited as NCMA 201708002.

362. The method of claim 300, wherein the purified bacterial population contains bacteria of the genus Klebsiella .

363. The method of claim 300, wherein the purified bacterial population contains bacteria of the species Klebsiella variicola .

364. The method of claim 300, wherein the purified bacterial population contains bacteria deposited as NCMA 201708001.

365. The method of claim 300, wherein the purified bacterial population contains bacteria deposited as NCMA 201712001.

366. The method of claim 300, wherein the purified bacterial population contains bacteria deposited as NCMA 201712002.

367. Isolated bacteria containing:

i. a 16S nucleic acid sequence that is at least about 97% identical to a nucleic acid sequence selected from SEQ ID NOs: 85, 96, 111, 121, 122, 123, 124, 136, 149, 157, 167, 261, 262, 269, 277-283;

ii. a nucleic acid sequence that is at least about 90% identical to a nucleic acid sequence selected from SEQ ID NOs: 86-95, 97-110, 112-120, 125-135, 137-148, 150-156, 158-166 , 168-176, 263-268, 270-274, 275, 276, 284-295; and/or

iii. a nucleic acid sequence that is at least about 90% identical to a nucleic acid sequence selected from SEQ ID NOs: 177-260, 296-303.

368. The isolated bacteria of claim 367, comprising: a 16S nucleic acid sequence that is at least about 97% identical to a nucleic acid sequence selected from SEQ ID NOs: 85, 96, 111, 121, 122, 123, 124, 136 , 149, 157, 167, 261, 262, 269 and 277-283.

369. The isolated bacteria of claim 367, comprising: a 16S nucleic acid sequence that is at least about 99% identical to a nucleic acid sequence selected from SEQ ID NOs: 85, 96, 111, 121, 122, 123, 124, 136 , 149, 157, 167, 261, 262, 269 and 277-283.

370. Isolated bacteria according to claim 367, containing: a 16S nucleic acid sequence selected from SEQ ID NOs: 85, 96, 111, 121, 122, 123, 124, 136, 149, 157, 167, 261, 262, 269 and 277-283.

371. The isolated bacteria of claim 367, comprising: a nucleic acid sequence that is at least about 90% identical to a nucleic acid sequence selected from SEQ ID NOs: 86-95, 97-110, 112-120, 125-135, 137-148, 150-156, 158-166, 168-176, 263-268, 270-274, 275, 276 and 284-295.

372. The isolated bacteria of claim 367, comprising: a nucleic acid sequence that is at least about 95% identical to a nucleic acid sequence selected from SEQ ID NOs: 86-95, 97-110, 112-120, 125-135, 137-148, 150-156, 158-166, 168-176, 263-268, 270-274, 275, 276 and 284-295.

373. The isolated bacteria of claim 367, comprising: a nucleic acid sequence that is at least about 99% identical to a nucleic acid sequence selected from SEQ ID NOs: 86-95, 97-110, 112-120, 125-135, 137-148, 150-156, 158-166, 168-176, 263-268, 270-274, 275, 276 and 284-295.

374. Isolated bacteria according to claim 367, containing: a nucleic acid sequence selected from SEQ ID NOs: 86-95, 97-110, 112-120, 125-135, 137-148, 150-156, 158-166, 168 -176, 263-268, 270-274, 275, 276 and 284-295.

375. The isolated bacteria of claim 367, comprising: a nucleic acid sequence that is at least about 90% identical to a nucleic acid sequence selected from SEQ ID NOs: 177-260 and 296-303.

376. The isolated bacteria of claim 367, comprising: a nucleic acid sequence that is at least about 95% identical to a nucleic acid sequence selected from SEQ ID NOs: 177-260 and 296-303.

377. The isolated bacteria of claim 367, comprising: a nucleic acid sequence that is at least about 99% identical to a nucleic acid sequence selected from SEQ ID NOs: 177-260 and 296-303.

378. Isolated bacteria according to claim 367, containing: a nucleic acid sequence selected from SEQ ID NOs: 177-260 and 296-303.

379. Isolated bacteria according to claim 367, containing: at least one genetic variation introduced into a gene selected from the group consisting of nifA , nifL , ntrB , ntrC , a polynucleotide encoding glutamine synthetase, glnA , glnB , glnK , drat, amtB , a polynucleotide encoding glutaminase, glnD , glnE , nifJ , nifH , nifD , nifK , nifY , nifE , nifN , nifU , nifS , nifV , nifW , nifZ , nifM , nifF , nifB , nifQ and a gene associated with the biosynthesis of the nitrogenase enzyme.

380. Isolated bacteria according to claim 367, containing: at least one genetic variation introduced into a gene selected from the group consisting of nifA , nifL , ntrB , ntrC , a polynucleotide encoding glutamine synthetase, glnA , glnB , glnK , drat, amtB , a polynucleotide encoding glutaminase, glnD , glnE , nifJ , nifH , nifD , nifK , nifY , nifE , nifN , nifU , nifS , nifV , nifW , nifZ , nifM , nifF , nifB , nifQ and a gene associated with the biosynthesis of the nitrogenase enzyme, where the genetic variation (A) is a knockout mutation; (B) removes or eliminates the regulatory sequence of the target gene; (C) contains an insert of a heterologous regulatory sequence or (D) is a domain deletion.

381. Selected bacteria according to item 367, containing: at least one mutation that leads to one or more of: increased expression or activity of NifA or glutaminase; decreased expression or activity of nifL, NtrB, glutamine synthetase, GlnB, GlnK, DraT, or AmtB; reduced GlnE adenylyl scavenging activity; or reduced GlnD uridylate scavenging activity.

382. Isolated bacteria according to claim 367, containing: a mutant nifL gene that is modified to contain a heterologous promoter inserted into said nifL gene.

383. Isolated bacteria according to item 367, containing: a mutant glnE gene, which leads to the formation of a truncated glnE protein with the absence of an adenylyl-removing (AR) domain.

384. Isolated bacteria according to item 367, containing: a mutant amtB gene, which leads to the absence of the expression of the specified amtB gene.

385. The isolated bacteria of claim 367, containing: at least one of the following genetic alterations: a mutant nifL gene that is modified to contain a heterologous promoter inserted into said nifL gene; a mutant glnE gene that results in a truncated glnE protein lacking an adenylyl-removal (AR) domain; a mutant amtB gene that results in no expression of said amtB gene; and their combinations.

386. Isolated bacteria according to claim 367, containing: a mutant nifL gene, which is modified to contain a heterologous promoter inserted into the specified nifL gene and a mutant glnE gene, which leads to the formation of a truncated glnE protein with the absence of an adenylyl-removing (AR) domain .

387. Isolated bacteria according to claim 367, containing: a mutant nifL gene that is modified to contain a heterologous promoter inserted into the specified nifL gene and a mutant glnE gene that leads to the formation of a truncated glnE protein with the absence of an adenylyl-removing (AR) domain and a mutant amtB gene that results in the lack of expression of said amtB gene.

388. The isolated bacteria of claim 367 containing: the nifL modification which express the NifL protein in less than about 50% of the bacteria with no nifL modification.

389. The isolated bacteria of claim 367, comprising: a nifL modification that expresses the NifL protein in less than about 50% of bacteria with no nifL modification, where the modification is disruption, knockout, or truncation.

390. The isolated bacteria of claim 367, comprising: a nifL modification that expresses the NifL protein in less than about 50% of the bacteria without the nifL modification, and wherein the bacteria additionally contain a promoter operably linked to the nifA sequence.

391. The isolated bacteria of claim 367 containing: a nifL modification that expresses the NifL protein in less than about 50% of the bacteria with no nifL modification, and where the bacteria lacks a nifL homologue.

392. The isolated bacteria of claim 367 containing: a glnE modification that express the glnE protein in less than about 50% of bacteria with no glnE modification.

393. The isolated bacteria of claim 367, comprising: a glnE modification that expresses the glnE protein in less than about 50% of bacteria with no glnE modification, where the modification is disruption, knockout, or truncation.

394. The isolated bacteria of claim 367 containing: a glnE modification that express the glnE protein in less than about 50% of bacteria without the glnE modification, and where the glnE protein sequence lacks an adenylyl-removal (AR) domain.

395. The isolated bacteria of claim 367 containing: a glnE modification that express the glnE protein in less than about 50% of the bacteria without the glnE modification, and where the glnE protein sequence lacks adenylyl-clearing (AR) activity.

396. The isolated bacteria of claim 367, wherein the bacteria, in planta , provide the plant with at least 1% of the fixed nitrogen that affects said bacteria.

397. The isolated bacteria of claim 367, wherein the bacteria, in planta , provide the plant with at least 5% of the fixed nitrogen that affects said bacteria.

398. The isolated bacteria of claim 367, wherein the bacteria, in planta , provide the plant with at least 10% of the fixed nitrogen that affects said bacteria.

399. The isolated bacteria of claim 367, wherein the bacteria are capable of fixing atmospheric nitrogen under non-nitrogen-limiting conditions.

400. The isolated bacteria of claim 367, wherein the bacteria, in planta , excretes nitrogen-containing products of nitrogen fixation.

401. The isolated bacteria of claim 367, wherein the bacteria, in planta , provide the plant with at least 1% of the fixed nitrogen affecting said bacteria, and wherein said fixed nitrogen is measured by diluting the enriched fertilizer with atmospheric N ₂ gas in the plant tissue.

402. The isolated bacteria of claim 367, wherein the bacteria colonize the root of a plant that attacks said bacteria at a concentration of at least about 1.0 x 10 ⁴ bacterial cells per gram wet weight of plant root tissue.

403. The isolated bacteria of claim 367 formulated into an agricultural composition.

404. The isolated bacteria of claim 367 formulated into a furrow-applied composition.

405. The isolated bacteria of claim 367, formulated as a liquid furrow composition that contains bacteria at a concentration of about 1 x 10 ⁷ to about 1 x 10 ¹⁰ CFU per milliliter.

406. The isolated bacteria of claim 367 formulated as a seed impregnation or seed coating.

407. The isolated bacteria of claim 367, formulated as a seed impregnation or seed coating, which contains the bacteria at a concentration of from about 1 x 10 ⁵ to about 1 x 10 ⁷ cfu per seed.

408. The isolated bacteria of claim 367, wherein the bacteria contact the plant and increase nitrogen fixation in the plant.

409. The isolated bacteria of claim 367 applied to a non-legume plant.

410. Isolated bacteria according to item 367 applied to a cereal crop.

411. The isolated bacteria of claim 367 applied to a plant selected from the group consisting of: corn, rice, wheat, barley, sorghum, millet, oats, rye and triticale.

412. Isolated bacteria according to item 367 applied to corn.

413. Isolated bacteria according to item 367 applied to a leguminous plant.

414. Isolated bacteria according to item 367 applied to a grain crop.

415. Isolated bacteria according to item 367, selected from: Rahnella aquatilis , Klebsiella variicola , Achromobacter spiritinus , Achromobacter marplatensis , Microbacterium murale , Kluyvera intermedia , Kosakonia pseudosacchari , Enterobacter species, Azospirillum lipoferum , Kosakonia sacchari and their combinations.

416. The isolated bacteria of claim 367, wherein the bacteria belong to the genus Rahnella .

417. Isolated bacteria according to item 367, where the bacteria belong to the species Rahnella aquatilis .

418. Isolated bacteria according to item 367, deposited as PTA-122293.

419. The isolated bacteria of claim 367, deposited as NCMA 201701003.

420. The isolated bacteria of claim 367, wherein the bacteria belong to the genus Kosakonia .

421. The isolated bacteria of claim 367, wherein the bacteria are of the species Kosakonia sacchari .

422. Isolated bacteria according to item 367, deposited as PTA-122294.

423. The isolated bacteria of item 367, deposited as NCMA 201701001.

424. The isolated bacteria of claim 367, deposited as NCMA 201701002.

425. The isolated bacteria of claim 367, deposited as NCMA 201708004.

426. The isolated bacteria of claim 367, deposited as NCMA 201708003.

427. The isolated bacteria of item 367, deposited as NCMA 201708002.

428. The isolated bacteria of claim 367, wherein the bacteria belong to the genus Klebsiella .

429. The isolated bacteria of claim 367, wherein the bacteria belong to the species Klebsiella variicola .

430. The isolated bacteria of item 367, deposited as NCMA 201708001.

431. The isolated bacteria of item 367, deposited as NCMA 201712001.

432. The isolated bacteria of item 367, deposited as NCMA 201712002.

433. Composition containing any one or more bacteria according to paragraphs. 415-432.

434. A method for detecting a sequence with a non-natural compound, comprising: amplifying a nucleotide sequence that is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79% , 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96 %, 97%, 98%, 99% or 100% identical to the sequence with SEQ ID NO: 372-405.

435. The method according to p. 434, where the specified amplification is carried out by polymerase chain reaction.

436. The method according to p. 434, where the specified amplification is carried out by quantitative polymerase chain reaction.

437. A method for detecting a sequence with a non-natural compound, comprising: amplifying a nucleotide sequence that is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79% , 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96 %, 97%, 98%, 99% or 100% identical to the sequence of a fragment with at least 10 consecutive base pairs contained in SEQ ID NO: 372-405, where the specified fragment with consecutive base pairs consists of nucleotides at the intersection: located above sequence containing SEQ ID NO: 304-337, and below the sequence containing SEQ ID NO: 338-371.

438. The method according to p. 437, where the specified amplification is carried out by polymerase chain reaction.

439. The method of claim 437, wherein said amplification is carried out by means of a quantitative polymerase chain reaction.

440. A sequence with a non-natural compound containing: a nucleotide sequence that is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80% , 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97 %, 98%, 99% or 100% identical to the sequence with SEQ ID NO: 372-405.

441. A sequence with a non-natural compound containing: a nucleotide sequence that is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80% , 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97 %, 98%, 99%, or 100% identical to the sequence of a fragment with at least 10 consecutive base pairs contained in SEQ ID NO: 372-405, where the specified fragment with consecutive base pairs consists of nucleotides at the intersection: located above the sequence containing SEQ ID NO: 304-337, and the sequence below containing SEQ ID NO: 338-371.

442. Bacterial composition containing:

at least one reconstructed bacterial strain that fixes atmospheric nitrogen, at least one reconstructed bacterial strain containing exogenously added DNA, where said exogenously added DNA is at least 80% identical with the corresponding native bacterial strain.

443. A method of maintaining nitrogen levels in the soil, including:

planting in the soil of a crop field inoculated with reshaped bacteria that fix atmospheric nitrogen; And

harvesting said crop, wherein between planting and harvesting, no more than 90% of the dose of nitrogen required to produce said crop is applied to said soil of said field.

444. A method for delivering a probiotic supplement to an agricultural crop, including:

coating the seeds of the crop with a seed coating, seed dressing, or seed dressing, wherein said seed coat, seed dressing, or seed dressing contains live representatives of said probiotic; And

the introduction of these seeds of agricultural crops into the soil of the field.

445. A method for increasing the amount of nitrogen obtained from the atmosphere in a non-legume plant, including:

exposing said non-legume plant to reshaped non-intergeneric microorganisms, wherein said reengineered non-intergeneric microorganisms carry at least one genetic variation introduced into the nitrogen fixation genetic regulatory network or at least one genetic variation introduced into the nitrogen assimilation genetic regulatory network.

446. A method for increasing the amount of nitrogen obtained from the atmosphere in a corn plant, including:

exposing said corn plant to reshaped non-intergeneric microorganisms carrying remodeled genetic variations in at least two genes selected from the group consisting of nifL , glnB , glnE and amtB .

447. A method for increasing the amount of nitrogen obtained from the atmosphere in a corn plant, including:

exposing said corn plant to reengineered non-intergeneric microorganisms carrying at least one genetic variation introduced into the nitrogen fixation genetic regulatory network and at least one genetic variation introduced into the nitrogen assimilation genetic regulatory network, wherein said reengineered non-intergeneric microorganisms , in planta , produce at least 5% fixed nitrogen in said corn plant, as measured by ¹⁵ N dilution in crops grown in fields treated with a fertilizer containing 1.2% ¹⁵ N.

448. The method of any one of the preceding claims, wherein the bacterium produces a fixed N in an amount of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour in the presence of exogenous nitrogen.

449. A bacterium according to any of the preceding claims, wherein the bacterium produces a fixed N in an amount of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour in the presence of exogenous nitrogen.

450. A population of bacteria according to any one of the preceding claims, wherein the bacterium produces a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour in the presence of exogenous nitrogen.

451. The isolated bacteria of any one of the preceding claims, wherein the bacterium produces a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour in the presence of exogenous nitrogen.

452. A non-intergeneric population of bacteria according to any one of the preceding claims, wherein the bacterium produces a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour in the presence of exogenous nitrogen.

453. The non-intergeneric bacterium of any one of the preceding claims, wherein the bacterium produces a fixed N of at least about 1 x 10 ^-17 mmol N per bacterial cell per hour in the presence of exogenous nitrogen.

454. The method of any one of the preceding claims, wherein the plurality of bacteria, in planta , produce 5% or more fixed nitrogen in the plant.

455. The method of any one of the preceding claims, wherein the plurality of non-intergeneric bacteria, in planta , produces 5% or more of fixed nitrogen in the plant.

456. A bacterium according to any one of the preceding claims, wherein the plurality of bacteria, in planta , produce 5% or more of fixed nitrogen in the plant.

457. A bacterial population according to any one of the preceding claims, wherein the plurality of bacteria, in planta , produces 5% or more fixed nitrogen in the plant.

458. An isolated bacteria according to any one of the preceding claims, wherein the plurality of bacteria, in planta , produce 5% or more of fixed nitrogen in the plant.

459. A non-intergeneric population of bacteria according to any one of the preceding claims, wherein the plurality of non-intergeneric bacteria, in planta , produces 5% or more fixed nitrogen in the plant.

460. The non-intergeneric bacterium of any one of the preceding claims, wherein the plurality of non-intergeneric bacteria, in planta , produce 5% or more of fixed nitrogen in the plant.

461. The method of any one of the preceding claims, wherein the plurality of bacteria, in planta , produce 10% or more fixed nitrogen in the plant.

462. The method of any one of the preceding claims, wherein the plurality of non-intergeneric bacteria, in planta , produces 10% or more fixed nitrogen in the plant.

463. A bacterium according to any one of the preceding claims, wherein the plurality of bacteria, in planta , produce 10% or more fixed nitrogen in the plant.

464. A population of bacteria according to any one of the preceding claims, wherein the plurality of bacteria, in planta , produce 10% or more of fixed nitrogen in the plant.

465. Isolated bacteria according to any one of the preceding claims, wherein the plurality of bacteria, in planta , produce 10% or more fixed nitrogen in the plant.

466. A non-intergeneric population of bacteria according to any one of the preceding claims, wherein the plurality of non-intergeneric bacteria, in planta , produces 10% or more fixed nitrogen in the plant.

467. The non-intergeneric bacterium of any one of the preceding claims, wherein the plurality of non-intergeneric bacteria, in planta , produce 10% or more of fixed nitrogen in the plant.

468. The method of any one of the preceding claims, wherein the plurality of bacteria, in planta , produce 15% or more fixed nitrogen in the plant.

469. The method of any one of the preceding claims, wherein the plurality of non-intergeneric bacteria, in planta , produce 15% or more fixed nitrogen in the plant.

470. A bacterium according to any one of the preceding claims, wherein the plurality of bacteria, in planta , produce 15% or more of fixed nitrogen in the plant.

471. A bacterial population according to any one of the preceding claims, wherein the plurality of bacteria, in planta , produce 15% or more of fixed nitrogen in the plant.

472. An isolated bacteria according to any one of the preceding claims, wherein the plurality of bacteria, in planta , produce 15% or more fixed nitrogen in the plant.

473. A non-intergeneric bacterial population according to any one of the preceding claims, wherein the plurality of non-intergeneric bacteria, in planta , produces 15% or more of fixed nitrogen in the plant.

474. The non-intergeneric bacterium of any one of the preceding claims, wherein the plurality of non-intergeneric bacteria, in planta , produces 15% or more of fixed nitrogen in the plant.

475. The method of any one of the preceding claims, wherein the plurality of bacteria, in planta , produce 20% or more of fixed nitrogen in the plant.

476. The method of any one of the preceding claims, wherein the plurality of non-intergeneric bacteria, in planta , produces 20% or more fixed nitrogen in the plant.

477. A bacterium according to any one of the preceding claims, wherein the plurality of bacteria, in planta , produce 20% or more fixed nitrogen in the plant.

478. A bacterial population according to any one of the preceding claims, wherein the plurality of bacteria, in planta , produces 20% or more fixed nitrogen in the plant.

479. An isolated bacteria according to any one of the preceding claims, wherein the plurality of bacteria, in planta , produce 20% or more of fixed nitrogen in the plant.

480. A non-intergeneric bacterial population according to any one of the preceding claims, wherein the plurality of non-intergeneric bacteria, in planta , produces 20% or more of fixed nitrogen in the plant.

481. The non-intergeneric bacterium of any one of the preceding claims, wherein the plurality of non-intergeneric bacteria, in planta , produces 20% or more of fixed nitrogen in the plant.

482. The method according to any one of the preceding claims, wherein the product of (i) the average colonizing capacity per unit of plant root tissue and (ii) the fixed N produced per bacterial cell per hour is at least about 2.0 x 10 ^-7 mmol N per grams wet weight of plant root tissue per hour.

483. A bacterium according to any of the preceding claims, wherein the product of (i) the average colonizing capacity per unit of plant root tissue and (ii) the fixed N produced per bacterial cell per hour is at least about 2.0 x 10 ^-7 mmol N per grams wet weight of plant root tissue per hour.

484. A population of bacteria according to any one of the preceding claims, wherein the product of (i) the average colonizing capacity per unit root tissue of the plant and (ii) the fixed N produced per bacterial cell per hour is at least about 2.0 x 10 ^-7 mmol N per gram wet weight of plant root tissue per hour.

485. The isolated bacteria of any one of the preceding claims, wherein the product of (i) the average colonizing capacity per unit of plant root tissue and (ii) the fixed N produced per bacterial cell per hour is at least about 2.0 x 10 ^-7 mmol N per gram wet weight of plant root tissue per hour.

486. A non-intergeneric population of bacteria according to any of the preceding claims, wherein the product of (i) the average colonizing capacity per unit root tissue of the plant and (ii) the fixed N produced per bacterial cell per hour is at least about 2.0 × 10 ^{- 7} mmol N per gram wet weight of plant root tissue per hour.

487. A non-intergeneric bacterium according to any one of the preceding claims, wherein the product of (i) the average colonizing capacity per unit root tissue of the plant and (ii) the fixed N produced per bacterial cell per hour is at least about 2.0 x 10 ^-7 mmol N per gram wet weight of plant root tissue per hour.

488. The method according to any one of the preceding claims, wherein the product of (i) the average colonizing capacity per unit of plant root tissue and (ii) the fixed N produced per bacterial cell per hour is at least about 2.0 x 10 ^-6 mmol N per grams wet weight of plant root tissue per hour.

489. A bacterium according to any of the preceding claims, wherein the product of (i) the average colonizing capacity per unit of plant root tissue and (ii) the fixed N produced per bacterial cell per hour is at least about 2.0 x 10 ^-6 mmol N per grams wet weight of plant root tissue per hour.

490. A population of bacteria according to any one of the preceding claims, wherein the product of (i) the average colonizing capacity per unit of plant root tissue and (ii) the fixed N produced per bacterial cell per hour is at least about 2.0 x 10 ^-6 mmol N per gram wet weight of plant root tissue per hour.

491. The isolated bacteria of any one of the preceding claims, wherein the product of (i) the average colonizing capacity per unit of plant root tissue and (ii) the fixed N produced per bacterial cell per hour is at least about 2.0 x 10 ^-6 mmol N per gram wet weight of plant root tissue per hour.

492. A non-intergeneric population of bacteria according to any one of the preceding claims, wherein the product of (i) the average colonizing capacity per unit of plant root tissue and (ii) the fixed N produced per bacterial cell per hour is at least about 2.0 × 10 ^{- 6} mmol N per gram wet weight of plant root tissue per hour.

493. A non-intergeneric bacterium according to any one of the preceding claims, wherein the product of (i) the average colonizing capacity per unit root tissue of the plant and (ii) the fixed N produced per bacterial cell per hour is at least about 2.0 × 10 ^-6 mmol N per gram wet weight of plant root tissue per hour.

494. The method of any one of the preceding claims, wherein the plant is remodeled or bred to use nitrogen efficiently.

495. A bacterial composition according to any one of the preceding claims, wherein said at least one reconstructed bacterial strain contains at least one variation in a gene or intergenic region within 10,000 bp. from a gene selected from the group consisting of: nifA, nifL, ntrB, ntrC, glnA, glnB, glnK, draT, amtB, glnD, glnE, nifJ, nifH, nifD, nifK, nifY, nifE, nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, and nifQ.

496. The method according to any of the previous paragraphs, where the specified reconstructed bacterium contains a bacterial composition according to any one of the preceding paragraphs.

497. The method according to any of the previous paragraphs, where the specified reconstructed bacterium consists of a bacterial composition according to any of the preceding paragraphs.

498. The method of any one of the preceding claims, wherein the reshaped bacterial strain is a reshaped Gram-positive bacterial strain.

499. The method according to any one of the preceding claims, wherein the reshaped Gram-positive bacterial strain has an altered expression level of the Nif cluster regulator.

500. The method of any one of the preceding claims, wherein the reshaped Gram-positive bacterial strain expresses a reduced amount of a negative Nif cassette regulator.

501. The method of any one of the preceding claims, wherein the reshaped bacterial strain expresses a reduced amount of GlnR .

502. The method according to any of the preceding claims, wherein the genome of the reconstructed bacterial strain encodes a polypeptide that is at least 75% identical to the sequence from the NifH database of the laboratory of J. Zehr.

503. The method according to any one of the preceding claims, wherein the genome of the reconstructed bacterial strain encodes a polypeptide that is at least 85% identical to the sequence from the NifH database of the laboratory of J. Zehr.

504. The method according to any one of the preceding claims, wherein the genome of the reconstructed bacterial strain encodes a polypeptide that is at least 75% identical to a sequence from the NifH database of D. Buckley's laboratory.

505. The method according to any one of the preceding claims, wherein the genome of the reconstructed bacterial strain encodes a polypeptide that is at least 85% identical to the sequence from the NifH database of D. Buckley's laboratory.

506. The method of any one of the preceding claims, wherein said reshaped non-intergeneric microorganisms carry at least one genetic variation introduced into said nitrogen fixation genetic regulatory network.

507. The method of any one of the preceding claims, wherein said reshaped non-intergeneric microorganisms carry at least one genetic variation introduced into said nitrogen assimilation genetic regulatory network.

508. The method of any one of the preceding claims, wherein said reshaped non-intergeneric microorganisms carry at least one genetic variation introduced into said nitrogen fixation genetic regulatory network and at least one genetic variation introduced into said nitrogen assimilation genetic regulatory network .

509. The method of any one of the preceding claims, wherein said reshaped non-intergeneric microorganisms are applied to the furrows in which the seeds of said non-legume plant are planted.

510. The method of any one of the preceding claims, wherein said reshaped non-intergeneric microorganisms are applied to the seeds of said non-legume plant.

511. The method according to any one of the preceding claims, wherein said reshaped non-intergeneric microorganisms colonize at least the root of said non-legume plant such that said remodeled non-intergeneric micro-organisms are present in said non-legume plant in an amount of at least 10 ⁵ colony forming units per gram wet mass of tissue.

512. The method of any one of the preceding claims, wherein said reshaped non-intergeneric microorganisms are capable of fixing atmospheric nitrogen under non-nitrogen-limiting conditions.

513. The method of any one of the preceding claims, wherein said reshaped non-intergeneric microorganisms, in planta , excrete nitrogen-containing nitrogen fixation products.

514. The method according to any one of the preceding claims, wherein said reshaped non-intergeneric microorganisms, in planta , produce at least 1% fixed nitrogen in said non-legume plant.

515. The method according to any one of the preceding claims, wherein said fixed nitrogen in said non-legume plant produced by said reconstructed non-intergeneric microorganisms is determined by diluting ¹⁵ N in crops grown in fields treated with a fertilizer containing 1.2% ¹⁵ N.

516. The method of any one of the preceding claims, wherein said reshaped non-intergeneric microorganisms, in planta , produce 5% or more of fixed nitrogen in said non-legume plant.

517. The method of any one of the preceding claims, wherein said non-intergeneric microorganisms are engineered using at least one type of engineering selected from the group consisting of site-directed mutagenesis, random mutagenesis, and site-directed evolution.

518. The method of any one of the preceding claims, wherein said reshaped non-intergeneric microorganisms, in planta , excrete nitrogen-containing nitrogen fixation products.

519. The method of any one of the preceding claims, wherein said reshaped non-intergeneric microorganisms are applied to furrows in which seeds of said corn plant are planted.

520. The method of any one of the preceding claims, wherein said reshaped non-intergeneric microorganisms are applied to seeds of said corn plant.

521. The method according to any one of the preceding claims, wherein said reconstructed non-intergeneric microorganisms carry at least one genetic variation introduced into the nitrogen fixation genetic regulatory network and at least one genetic variation introduced into the nitrogen assimilation genetic regulatory network.

522. The method of any one of the preceding claims, wherein said non-intergeneric microorganisms carry at least one genetic variation introduced into the nitrogen fixation genetic regulatory network and at least one genetic variation introduced into the nitrogen assimilation genetic regulatory network.

523. The method according to any one of the preceding claims, wherein said genetically engineered non-intergeneric microorganisms carry at least one genetic variation introduced into the nitrogen fixation genetic regulatory network and at least one genetic variation introduced into the nitrogen assimilation genetic regulatory network .

524. The method of any one of the preceding claims, wherein said reshaped non-intergeneric bacteria carry at least one genetic variation introduced into the nitrogen fixation genetic regulatory network and at least one genetic variation introduced into the nitrogen assimilation genetic regulatory network.

525. The method of any one of the preceding claims, wherein said non-intergeneric bacteria carry at least one genetic variation introduced into the nitrogen fixation genetic regulatory network and at least one genetic variation introduced into the nitrogen assimilation genetic regulatory network.

526. The method according to any one of the preceding claims, wherein said genetically engineered non-intergeneric bacteria carry at least one genetic variation introduced into the nitrogen fixation genetic regulatory network and at least one genetic variation introduced into the nitrogen assimilation genetic regulatory network .

527. A bacterium according to any one of the preceding claims, wherein said bacterium carries at least one genetic variation introduced into the nitrogen fixation genetic regulatory network and at least one genetic variation introduced into the nitrogen assimilation genetic regulatory network.

528. A bacterial population according to any one of the preceding claims, wherein the bacteria in said bacterial population carry at least one genetic variation introduced into the nitrogen fixation genetic regulatory network and at least one genetic variation introduced into the nitrogen assimilation genetic regulatory network.

529. The isolated bacteria according to any one of the preceding claims, wherein said isolated bacteria carry at least one genetic variation introduced into the nitrogen fixation genetic regulatory network and at least one genetic variation introduced into the nitrogen assimilation genetic regulatory network.

530. A non-intergeneric bacterial population according to any of the preceding claims, wherein the non-intergeneric bacteria in said non-intergeneric bacterial population carry at least one genetic variation introduced into the nitrogen fixation genetic regulatory network and at least one genetic variation, introduced into the genetic regulatory network of nitrogen assimilation.

531. A non-intergeneric bacterium according to any one of the preceding claims, wherein said non-intergeneric bacterium carries at least one genetic variation introduced into the nitrogen fixation genetic regulatory network and at least one genetic variation introduced into the nitrogen assimilation genetic regulatory network .

532. Composition containing any one or more bacteria according to any of the previous paragraphs.

--->

SEQUENCE LIST

<110> PIVOT BIO, INC.

<120> METHODS AND COMPOSITIONS FOR IMPROVING PLANT CHARACTERISTICS

<130> 47736-707.601

<140>

<141>

<150> 62/577.147

<151> 2017-10-25

<150> 62/566.199

<151> 2017-09-29

<150> 62/467.032

<151> 2017-03-03

<150> 62/447.889

<151> 2017-01-18

<150> 62/445.557

<151> 2017-01-12

<150> 62/445.570

<151> 2017-01-12

<160> 424

<170>PatentIn version 3.5

<210> 1

<211> 9

<212> Protein

<213> Unknown

<220>

<223> Description of unknown:

sequence of the peptide motif of the "LAGLIDADG" family

<400> 1

Leu Ala Gly Leu Ile Asp Ala Asp Gly

15

<210> 2

<211> 90

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic oligonucleotide

<400> 2

gttgatcaga ccgatgttcg gaccttccaa ggtttcgatc ggacatacgc gaccgtagtg 60

ggtcgggtgt acgtctcgaa cttcaaagcc 90

<210> 3

<211> 257

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 3

gcctctcggg gcgctttttt ttattccggc actagccgct attaataaaa atgcaaatcg 60

gaatttacta tttaacgcga gattatctaa gatgaatccg atggaagcgc gctgttttca 120

ctcgcctttt taaagttacg tgatgatttc gatgcttctt tgagcgaacg atcaaaaata 180

agcgtattca ggtaaaaaaa tattctcatc acaaaaaagt ttgtgtaata cttgtaacgc 240

tacatggaga ttaactc 257

<210> 4

<211> 260

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 4

ggttcacata aacataatta tcgccacggc gatagccgta cgctttttgc gtcacaacat 60

ccatggtgaa gccggctttt tcaagaacac gcgccacctc atcgggtctt aaatacatac 120

tcattcctca ttatctttta ccgcacgtta accttacctt attcattaaa ggcaacgctt 180

tcggaatatt ccataaaggg ctatttacag cataattcaa aatcttgtcc tacacttata 240

gactcaatgg aattaaggga 260

<210> 5

<211> 260

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 5

gcgcggaaaa tcgacgcata gcgcattctc agaagccggc ctggtctcgg tggaaaagcg 60

aatctttccc acgaccgccg ggcctttaac aaaagaatca atgacctgat taatgtcgct 120

atccattctc tctccgcgta atgcgatctt ttttcatcat acctaacaaa ctggcagagg 180

240

taacatatta ttgcaaacat 260

<210> 6

<211> 259

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 6

ctgacgaagc gagttacatc accggtgaaa ctctgcacgt caacggcgga atgtatatgg 60

tctgaccgag atttgcgcaa aacgctcagg aaccgcgcag tctgtgcggt tcactgtaat 120

gttttgtaca aaatgatttg cgttatgagg gcaaacagcc gcaaaatagc gtaaaatcgt 180

ggtaagacct gccgggattt agttgcaaat ttttcaacat tttatacact acgaaaacca 240

tcgcgaaagc gagttttga 259

<210> 7

<211> 260

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 7

acgcctgggg cgccgaccag cgggaagagt gatttggcca acgaggcgcc gctctgaatg 60

gaaatcatgg cgattaaaat aaccagtatc ggcaaccatg ccggtacctt acgagacgag 120

ccgggcatcc tttctcctgt caattttgtc aaatgcggta aaggttccag tgtaattgaa 180

ttaccccgcg ccggttgagc taatgttgaa aaaaagggtc ttaaaagcag tacaataggg 240

cgggtctgaa gataatttca 260

<210> 8

<211> 260

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 8

tctgattcct gatgaaaata aacgcgacct tgaagaaatt ccggataacg ttatcgccga 60

tttagatatc catccggtga aacgaatcga ggaagttctg gcacttgcgc tacagaacga 120

accgtttgga atggaagtcg tcacggcaaa atagtgattt cgcgcaaata gcgctaagaa 180

aaatagggct ggtaagtaaa ttcgtacttg ccagcctttt tttgtgtagc taacttagat 240

cgctggcagg ggggtcaatt 260

<210> 9

<211> 259

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 9

gtaagaaagt cggcctgcgt aaagcacgtc gtcgtcctca gttctccaaa cgttaattgt 60

tttctgctca cgcagaacaa tttgcgaaaa aacccgcttc ggcgggtttt tttatggata 120

aatttgccat ttttccctcta caaacgcccc attgttacca ctttttcagc atttccagaa 180

tcccctcacc acaacgtctt caaaatctgg taaactatca tccaattttc tgcccaaatg 240

caggtgattg ttcattttt 259

<210> 10

<211> 260

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 10

gtcaaagccg tattatcgac cccttagggga caacgcttgc cggggcggga gagcggccgc 60

agttgatttt tgccgaactt tcagctgatt atattcagca ggtacgcgag cgcctgccgg 120

180

tgattcacct tgttacagat tgctattgtg tccgcgcgtc aaatagccgt taattgtatg 240

cgtgtatgat ggcgtattcg 260

<210> 11

<211> 260

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 11

gaggcggtgg ttgaccgtat cggtcccgag catcatgagc tttcggggcg agcgaaagat 60

atgggatcgg cggcggtact gctggcgatt atcatcgcgc tgatcgcgtg gggaacgctg 120

ctgtgggcga actaccgcta agtcttgtcg tagctgctcg caaaacggaa agaaactcct 180

gatttttgtg tgaaatgtgg ttccaaaatc accgttagct gtatatactc acagcataac 240

tgtatataca ccggggggc 260

<210> 12

<211> 260

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 12

taagaaaagc ggcctgtacg aagacggcgt acgtaaagac aggctggata acgacgatat 60

gatcgatcag ctggaagcgc gtattcgcgc taaagcatcg atgctggatg aggcgcgtcg 120

180

atgattgaac ctgcgggcgc gaggcgccgg ggttcatttt tgtatatata aagagaataa 240

acgtggcaaa gaacattcaa 260

<210> 13

<211> 237

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 13

atgaatcgta ctaaactggt actgggcgcg gtaatcctgg gttctactct gctggctggt 60

tgctccagca atgctaaaat cgatcagctg tcttctgacg ttcagactct gaacgctaaa 120

gttgaccagc tgagcaacga cgtgaacgca atgcgttccg acgttcaggc tgctaaagat 180

gacgcagctc gcgctaacca gcgtctggac aacgcagcta ctaaataccg taagtaa 237

<210> 14

<211> 327

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 14

atggccaacc gagcaaaccg caacaacgta gaagagagcg ctgaagatat ccataacgat 60

gtcagccaat tagcggatac gctggaagag gtgctgaaat cgtggggcag cgacgccaaa 120

180

cttaacggca acaaccgcgt ccagcaggcg gcgtgcgacg ccatgggctg cgctgacagc 240

tacgtgcgcg acaaaccgtg gcaaagcgtc ggcgccgcag cagccgttgg ggtatttatt 300

ggcgtattac tgaatttacg tcgataa 327

<210> 15

<211> 648

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 15

atgaccaaaa agatttccgc cctagcgttt ggcattggca tggtaatggc gagcagccag 60

gcttttgccc acggtcacca tagtcatggc ccggcgctga ccgaagcgga acaaaaggcg 120

agtgaaggca tttttgctga ccaggacgta aaggacaggg cgctgagcga ctgggagggg 180

atctggcagt cggttaaccc ctatctgctg aacggggatt tagatccggt tctggagcag 240

aaggccaaaa aggccggtaa aagcgtggcg gaatatcggg aatattataa gaagggctac 300

360

accgtcaacg cctgtaagta cagctattcc ggttacaaaa ttctgaccta cgcatccggt 420

aaaaaaggcg tgcgctacct gttcgaatgc cagcaggcgg attcaaaagc gccgaagttt 480

gttcagttta gcgatcacac catcgcgcca cgcaagtccc agcatttcca catctttatg 540

ggcaatgagt ccggaagc gctgctgaaa gagatggata actggccaac ctactatcct 600

tatgcgctgc ataaagagca gattgtcgac gaaatgctgc accactaa 648

<210> 16

<211> 237

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 16

atgagcacta tcgaagaacg cgttaagaaa attatcggcg aacagctggg cgttaagcag 60

gaagaagtta ccaacaatgc ttccttcgtt gaagacctgg gcgctgattc tcttgacacc 120

gttgagctgg taatggctct ggaagaagag tttgatactg agattccggga cgaagaagct 180

gagaaaatca ctactgttca ggctgccatt gattacatca acggccacca ggcgtaa 237

<210> 17

<211> 513

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 17

atgaataaaa ttgcacgttt ttcagcactg gccgttgttc tggctgcatc cgtaggtacc 60

actgctttcg ctgcgacttc taccgttacc ggtggctacg cgcagagcga catgcagggt 120

gaagcgaaca aagctggcgg tttcaacctg aagtaccgct acgagcaaga caacaacccg 180

ctgggtgtta tcggttcttt cacctacacc gaaaaagatc gttctgaatc tggcgtttac 240

aaaaaaggcc agtactacgg catcaccgca ggtccggctt accgtctgaa cgactgggct 300

agcatctacg gcgtagtggg tgttggttac ggtaaattcc aggacaacag ctacccgaac 360

aaatctgata tgagcgacta cggtttctct tacggcgctg gtctgcagtt caacccgatc 420

gaaaacgttg ccctggactt ctcctacgag cagtctcgca ttcgtaacgt tgacgttggc 480

acctggattg ctggcgtaggg ttaccgcttc taa 513

<210> 18

<211> 273

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 18

gtgaataaat ctcaactgat tgacaaaatt gctgccggtg cggacatttc taaagccgca 60

gctggacgtg cgttagatgc tttaatcgct tctgttactg aatctctgca ggctggagat 120

gacgttgcgc tggtagggtt tggtactttt gctgttaaag agcgcgctgc ccgtactggt 180

cgcaatccgc aaacaggcaa agaaatcacc attgctgctg ctaaagttcc gggtttccgc 240

gcaggtaaag cgctgaaaga cgcggtaaac tga 273

<210> 19

<211> 639

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 19

atggctgtcg ctgccaacaa acgttcggta atgacgctgt tttctggtcc tactgacatc 60

tatagccatc aggtccgcat cgtgctggcc gaaaaaggtg ttagttttga gatagagcac 120

gtggagaagg acaacccgcc tcaggatctg attgacctca acccgaatca aagcgtaccg 180

acgcttgtgg atcgtgagct cactctgtgg gaatctcgca tcattatgga atatctggat 240

gagcgtttcc cgcatccgcc gctcatgccg gtttacccgg tggcgcgtgg ggaaagccgt 300

ctgtatatgc agcgtatcga aaaggactgg tattcgttga tgaataccat tcagaccggt 360

accgctgcgc aggctgatac tgcgcgtaag cagctgcgtg aagaactaca ggcgattgcg 420

ccagttttca cccagaagcc ctacttcctg agcgatgagt tcagcctggt ggactgctac 480

ctggcaccac tgctgtggcg tctgccggtt ctcggcgtag agctggtcgg cgctggcgcg 540

aaagagctta aaggctatat gactcgcgta tttgagcgcg actctttcct cgcttcttta 600

actgaagccg aacgtgaaat gcgtctcggt cggggctaa 639

<210> 20

<211> 204

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 20

atgggtgaga ttagtattac caaactgctg gtagtcgcag cgctgattat cctggtgttt 60

ggtaccaaaa agttacgcac gctgggtgga gacctgggct cggctatcaa aggctttaaa 120

aaagccatga gcgatgacga tgacagtgcg aagaagacca gtgctgaaga agcgccggca 180

cagaagctct ctcataaaga gtaa 204

<210> 21

<211> 609

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 21

atgaaagcgt taacgaccag gcagcaagag gtgtttgatc tcattcggga tcatatcagc 60

cagacgggca tgccgccgac gcgtgcggag attgctcagc gcttggggtt tcgctcccca 120

aacgcggcgg aagagcatct gaaagcgctg gcgcgtaaag gcgcaatcga gatcgtttcc 180

ggcgcctccc gcggtattcg tctgctgacg gaagaagaaa ccggtctgcc gcttattggc 240

cgcgtcgcgg caggtgagcc gctgctagcg cagcagcaca ttgaaggcca ctaccaggtg 300

gacccggcca tgtttaagcc gaacgccgat tttctgctgc gtgttagcgg tatgtcgatg 360

aaggatatcg gtattctcga tggcgacctg ctggctgtcc ataaaacgca ggatgtgcgc 420

aatggtcagg tggttgtggc gcgtatcgac gaagaagtga ccgtgaagcg tctgaaaaaa 480

cagggtaacg tcgtggaatt gctgccggaa aacagcgaat tctcgccgat cgtggtcgac 540

cttcgcgaac aaagctttac tattgaaggc ctggccgtcg gcgttatccg caacggcaac 600

tggcaataa 609

<210> 22

<211> 1245

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 22

atgaacgatt atctgccggg cgaaaccgct ctctggcagc gcattgaagg ctcactgaag 60

caggtgcttg gtagctacgg ttacagcgaa atccgtttgc cgattgtaga gcagaccccg 120

ttattcaaac gcgctatcgg cgaagtgacc gacgtggttg aaaaagagat gtacaccttt 180

gaggaccgta acggcgatag cctgactcta cgtccggaag gcacggctgg ctgcgtacgc 240

gccggtatcg aacatggtct cctgtacaat caagaacagc gcctgtggta cattgggccg 300

atgttccgcc acgaacgtcc gcaaaaaggc cgctaccgtc agttccacca gattggcgcc 360

gaagcgtttg gcctgcaggg gccggatatc gatgccgagc tgattatgct gaccgcccgc 420

tggtggcgcg agctgggcat ctccggccac gttgcgctgg agctgaactc tatcggttcg 480

ctggaggctc gcgctaacta tcgcgacgcg ctggtggcct atcttgagca gtttaaagat 540

aagctggacg aagactgcaa acgccgcatg tacaccaacc cgctgcgcgt gctggattct 600

aaaaacccgg acgtccaggc gctgctgaac gacgccccga cgctgggcga ctatcttgat 660

gaagagtcca aaacgcattt tgccgggctg tgcgcgctgc tggatgatgc cggtattcgc 720

tataccgtga atcagcgtct ggtacgcggt ctcgactact acaaccgcac cgtgtttgag 780

tgggtcacca ccagcctcgg ttcccagggc accgtctgcg ccgggaggccg ttacgatggt 840

ctggttgagc agcttggcgg tcgcgctacc cctggcgtcg gctttgcgat ggggctggaa 900

cgtcttgttt tactggttca ggcagtgaat cgggaattta aagccgatcc tgttgtcgat 960

atatacctgg tagcctccgg aactgacacc cagtccgcag caatgcgtct ggctgaacag 1020

gtacgcgatg cgttacccgg cgttaagctg atgaccaacc atggcggcgg caactttaag 1080

aagcagtttg cgcgcgctga taaatggggc gctcgcgttg cgctggtgct gggcgaatca 1140

gaaatcgccg acggaaacgt ggtagtgaaa gatttacgct caggtgagca aactaccgta 1200

acgcaggata gcgttgctgc gcatttgcgc acacttctgg gttaa 1245

<210> 23

<211> 1413

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 23

atgaaaaaga ccaaaattgt ttgcaccatc ggtccgaaaa ccgaatccga agagatgttg 60

accaaaatgc tggacgcggg catgaacgtt atgcgtctga acttctctca cggtgactat 120

gcggaacacg gtcagcgcat ccagaatctg cgcaatgtga tgagtaaaac cggtaagaaa 180

gcggcaatcc tgctggacac caaaggtccg gaaatccgta ccattaagct ggaaggcggc 240

aacgacgtct ccctgaaagc gggccagacc ttcaccttca ccaccgataa atccgttgtc 300

ggtaataacg aaatcgttgc ggtgacctat gaaggcttca ccagcgacct gagcgttggc 360

aacacggtac tggttgacga tggtctgatc ggtatggaag tgaccgctat cgaaggcaac 420

aaagttgttt gtaaagtgct gaacaacggc gacctcggcg agaacaaagg cgttaacctg 480

ccgggcgtat ctatcgcgct gccggcgctg gctgaaaaag acaaacagga tctgatcttc 540

ggttgcgaac agggcgttga ctttgttgcg gcatccttta tccgtaagcg ttctgacgtt 600

gttgaaatcc gtgagcacct gaaagcccac ggcggcgaga agatccagat catctccaaa 660

atcgaaaacc aggaaggcct gaacaacttc gacgaaatcc tcgaagcctc tgacggcatc 720

atggtagccc gtggcgacct gggcgttgaa atcccggttg aagaagttat cttcgcgcag 780

840

ctggattcca tgatcaaaaa cccgcgtccg acccgtgcgg aagcaggcga cgtggccaac 900

gccatcctcg acggcaccga cgcagttatg ctgtccggcg aatccgcgaa aggtaaatac 960

ccgctggaag cggtcaccat catggcgacc atctgcgaac gtaccgaccg cgtcatgacc 1020

agccgtcttg agtacaacaa cgacaaccgt aagctgcgca tcaccgaagc ggtgtgccgc 1080

ggtgcggtag aaacggctga aaaactggaa gcgccgctga tcgttgtggc aacccagggc 1140

ggtaaatccg cgcgcgccgt acgtaaatac ttcccggatg ccactatcct ggcgctgacc 1200

accaacgaaa ccaccgcgcg tcagctggtg ctgagcaaag gcgttgtggc acagctggtt 1260

gaagatatct cctctaccga tgcgttctac atccagggta aagaactggc gctgcagagc 1320

ggtctggcgc gtaaaggcga cgtggttgtt atggtttccg gcgcgttagt cccgagcgga 1380

accaccaata ccgcttccgt gcacgtgctg taa 1413

<210> 24

<211> 351

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 24

atgtatttaa gacccgatga ggtggcgcgt gttcttgaaa aagccggctt caccatggat 60

gttgtgacgc aaaaagcgta cggctatcgc cgtggcgata attatgttta tgtgaaccgt 120

gaagctcgta tggggcgtac cgcgttaatt attcatccgg ctttaaaaga gcgcagcaca 180

acgcttgcgg agcccgcgtc ggatatcaaa acctgcgatc attatgagca gttcccgctc 240

tatttagcgg gggatgctca acagcattat ggtattccac acgggttcag ttcgcgaatg 300

gcgcttgagc gttttctgag tggcctgttt ggcgaaacgc agtatagctg a 351

<210> 25

<211> 864

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 25

atggatagcg acattaatca ggtcattgat tcttttgtta aaggcccggc ggtcgtggga 60

aagattcgct tttccaccga gaccaggccg gcttctgaga atgcgctatg cgtcgatttt 120

ccgcgcctcg aaatcatgct tgcgggtcag cttcacgatc cggcgattaa agccgatcgc 180

gcccagctca tgccgcacga tgtgctgtat attcccgctg gcggatggaa tgacccgcaa 240

tggctggcgc cctccactct gctcactatc ttatttggta aacagcagct ggaattcgtc 300

ctgcgccact gggacggcag cgcgcttaac gtgctggata aacagcaggt tccgcgccgc 360

ggtccccggg tcggctcttt tctgctgcag gcgctgaatg aaatgcagat gcagccgcgg 420

gagcagcaca cggcccgctt tattgtcacc agcctgctca gccactgtgc cgatctgctg 480

ggcagccagg tacaaacctc atcgcgcagc caggcgcttt ttgaagcgat tcgtaagcat 540

attgacgccc actttgccga cccgttaacc cgggagtcgg tggcgcaggc gttttacctc 600

660

ctgaatcaca tccgcctgga gcaggccaga atgctgttaa aaggccacga tatgaaagtg 720

aaagatatcg cccacgcctg cggtttcgcc gacagcaact acttctgccg cctgtttcgc 780

aaaaacaccg aacgctcgcc gtcggagtat cgccgtcaat atcacagcca gctgacggaa 840

aaaacagccc cggcaaaaaa ctag 864

<210> 26

<211> 735

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 26

atgagttttg aaggaaaaat cgcgctggtt accggtgcaa gtcgcgggat tggccgcgca 60

atcgctgaaa cgctcgttgc ccgtggcgcg aaagttatcg ggactgcgac cagcgaaagc 120

ggcgcgcagg cgatcagcga ttatttaggt gctaacggta aaggtctgct gctgaatgtg 180

accgatcctg catctattga atctgttctg ggaaatattc gcgcagaatt tggtgaagtt 240

gatatcctgg tgaacaatgc cgggatcact cgtgataacc tgttaatgcg catgaaagat 300

gatgagtgga acgatattat cgaaaccaac ctgtcatctg ttttccgtct gtcaaaagcg 360

gtaatgcgcg ctatgatgaa aaagcgtcat ggacgtatta tcactatcgg ttctgtggtt 420

ggtaccatgg gaaatgcggg tcaggccaac tacgctgcgg cgaaagcggg tctgattggc 480

ttcagtaaat cactggctcg cgaagttgcg tcccgcggta ttactgtaaa cgttgttgct 540

ccgggcttta ttgaaacgga catgacgcgt gcgctgaccg atgagcagcg tgcgggtacg 600

ctggcggcag ttcctgcggg gcgcctcggc tctccaaatg aaatcgccag tgcggtggca 660

tttttagcct ctgacgaagc gagttacatc accggtgaaa ctctgcacgt caacggcgga 720

atgtatatgg tctga 735

<210> 27

<211> 71

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic oligonucleotide

<400> 27

atgcccggct cgtctcgtaa ggtaccggca tggttgccga tactggttat tttaatcgcc 60

atgatttcca t 71

<210> 28

<211> 2355

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 28

atgaatcctg agcgttctga acgcattgaa atccccgtat tgccgttgcg cgatgtggtg 60

gtttatccgc acatggtcat acccctgttt gtagggcggg aaaaatctat ccgttgtctc 120

gaagcagcca tggaccatga taaaaaaatc atgctggttg cgcagaaaga agcctcgacg 180

gatgagccgg gtgtaaacga tcttttcacc gtcgggaccg tggcgtctat tttgcaaatg 240

ctgaagctac cggacggtac tgttaaagtg ctggtcgaag gtttgcagcg cgcgcgcatc 300

tctgcgctgt ctgataatgg cgaacatttt tcggcgaagg cggaatacct tgaatcgccg 360

gcgattgacg aacgcgagca ggaagtgctg gttcgtaccg ctatcagcca gtttgaaggc 420

tacatcaagc tgaacaaaaa aatccctccg gaagtgctga cgtcgctgaa tagcatcgac 480

gatccggcgc gtctggcgga taccatcgct gcgcatatgc cgctgaagct ggcggacaaa 540

cagtccgtgc tggagatgtc cgacgttaac gagcgtctgg aatatctgat ggcgatgatg 600

gagtcggaaa tcgatctgct gcaggtggag aagcgtattc gcaaccgcgt gaaaaagcag 660

atggagaaat ctcagcgcga gtactatctg aatgagcaaa tgaaagccat tcaaaaagag 720

ctcggcgaga tggacgacgc cccggacgag aacgaagcgc tgaagcgtaa gatcgacgcg 780

gcgaaaatgc cgaaagaggc aaaagagaaa accgaagcgg aactgcaaaa actgaaaatg 840

atgtccccga tgtcggcgga agcgaccgtc gttcgcggct acatcgactg gatggtgcag 900

gtaccgtgga acgctcgcag caaggttaaa aaagacctgc gtcaggctca ggagatcctc 960

gataccgatc actacggcct tgagcgcgtg aaggatcgca ttcttgagta cctcgcggtg 1020

1080

ggtaaaacct ctctcggcca atccatcgcc aaagcaactg gacgcaaata tgtgcgtatg 1140

gcgctgggcg gcgtgcgtga tgaagcggaa atccgcggtc accgccgtac ctatattggc 1200

tcaatgccgg gcaaactgat ccagaaaatg gctaaagtgg gcgttaaaaa cccgctgttc 1260

ttgctggatg agatcgacaa gatgtcttct gacatgcgcg gcgatccggc ctcggcgctg 1320

ctggaggtgt tggatccgga acagaacgtg gcctttaacg accactatct ggaagtggat 1380

tacgatctca gcgacgtgat gttcgttgcg acctctaact ccatgaacat cccggcgccg 1440

ctgctggatc gtatggaagt gatccgcctc tccggctata ccgaagatga gaagctaaac 1500

atcgccaaac gccatctgct gtcaaaacag attgagcgta acgcgctcaa gaaaggcgag 1560

ctgacggtgg atgacagcgc gattatcggc atcattcgct actacacccg tgaagcaggc 1620

gtgcgtggtc tggagcgtga aatctcgaaa ctgtgccgca aagcggtgaa acagctgctg 1680

ctggataagt cgctgaaaca catcgagatt aacggcgaca acctgcacga tttccttggc 1740

gtgcagcgct acgactatgg tcgtgcggat agcgaaaacc gcgtaggtca ggtgaccgga 1800

1860

aaaggcaaac tgacctacac cggttcactg ggtgaagtca tgcaggaatc catccaggcg 1920

gcgctgacgg tggttcgttc acgtgcggat aagctgggta ttaactcaga cttttacgaa 1980

2040 aaacgtgata ttcacgttca cgtgccggaa

ggtatcgcga tgtgcaccgc gctggtttcc tgtctgacgg gtaatccggt acgcgccgac 2100

gtggcgatga ccggtgagat taccctccgt ggccaggtat tgccgattgg tggtctgaag 2160

gaaaaactgt tggccgcgca tcgcggcggc attaagactg ttctgattcc tgatgaaaat 2220

aaacgcgacc ttgaagaaat tccggataac gttatcgccg atttagatat ccatccggtg 2280

aaacgaatcg aggaagttct ggcacttgcg ctacagaacg aaccgtttgg aatggaagtc 2340

gtcacggcaa aatag 2355

<210> 29

<211> 393

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 29

atggctgaaa atcaatacta cggcaccggt cgccgcaaaa gttccgcagc tcgcgttttc 60

atcaaaccgg gcaacggtaa aatcgttatc aaccagcgtt ctctggaaca gtacttcggt 120

cgtgaaactg cccgcatggt agttcgtcag ccgctggaac tggtcgacat ggttgagaaa 180

ttagatctgt acatcaccgt taaaggtggt ggtatctctg gtcaggctgg tgcgatccgt 240

cacggtatca cccgcgctct gatggagtac gacgagtccc tgcgtggcga actgcgtaaa 300

gctggtttcg ttactcgtga tgctcgtcag gttgaacgta agaaagtcgg cctgcgtaaa 360

gcacgtcgtc gtcctcagtt ctccaaacgt taa 393

<210> 30

<211> 789

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 30

atgtttgttg ctgccggaca atttgccgta acgccggact ggacgggaaa cgcgcagacc 60

tgcgtcagca tgatgcgcca ggccgcggag cggggggcgt cgcttctggt tctgcctgag 120

180

gatggcggct tcttacagct cttgctggcg gagagcgaaa acagcgcttt gacgacggtg 240

ctgaccctgc atatcccttc cggcgaaggt cgagcgacga atacgctggt ggccctgcgt 300

caggggaaga ttgtggcgca atatcagaaa ctgcatctct atgatgcgtt caatatccag 360

gaatccaggc tggtcgatgc cgggcggcaa attccgccgc tgatcgaagt cgacgggatg 420

cgcgtcgggc tgatgacctg ctacgattta cgtttccctg agctggcgct gtcgttagcg 480

ctcagcggcg cgcagctcat agtgttgcct gccgcgtggg taaaagggcc gctgaaggaa 540

catcactggg cgacgctgct ggcggcgcgg gcgctggata caacctgcta tattgtcgcc 600

660 gcaggagagt gcgggacgcg taatatcggt

acgcttgccg gggcgggaga gcggccgcag ttgatttttg ccgaactttc agctgattat 720

attcagcagg tacgcgagcg cctgccggtg ttgcgcaatc gccgctttgc gccaccgcaa 780

ttattatga 789

<210> 31

<211> 369

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 31

atggccaata ataccactgg gttaacccga attattaaag cggccgggta ttcctggaaa 60

ggattccgtg cggcgtgggt caatgaggcc gcatttcgtc aggaaggcat cgcggccgtt 120

attgccgtgg cgatcgcctg ctggttggac gtcgatgcca tcacgcgggt gctgctcatt 180

agctcggtcc tgttagtgat gatagttgaa attatcaata gcgcgattga ggcggtggtt 240

gaccgtatcg gtcccgagca tcatgagctt tcggggcgag cgaaagatat gggatcggcg 300

gcggtactgc tggcgattat catcgcgctg atcgcgtggg gaacgctgct gtgggcgaac 360

taccgctaa 369

<210> 32

<211> 1122

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 32

atgcataacc aggctccgat tcaacgtaga aaatcaaaac gaatttacgt tgggaatgtg 60

ccgattggcg atggcgcccc catcgccgta cagtcgatga caaacacgcg caccaccgat 120

gtggcggcga cggtaaatca aattaaagcc ctcgagcgcg ttggcgcgga tatcgtgcgc 180

240

gtcccgctgg ttgccgatat ccacttcgat taccgcattg cgctgaaggt agcggaatac 300

ggcgttgatt gcctgcgtat taacccgggc aatatcggca acgaagagcg tatccgcatg 360

gtggtggact gcgctcgcga taaaaatatt cctatccgta tcggggtaaa cgccggttct 420

ctggaaaaag atctccagga aaaatacggc gaaccgactc cgcaggcgct gctggaatcg 480

gcaatgcgcc atgttgatca tctcgatcgt ctcaacttcg atcagtttaa agtcagcgta 540

aaagcctccg atgtgttcct cgcggttgaa tcctatcgcc tgttggcgaa acagatcgat 600

cagcctctgc acctcgggat caccgaagcg ggcggcgcgc gcagcggcgc ggtgaagtcc 660

gcgatcggcc tcggcctgct gctgtctgaa gggattggcg atacgctgcg cgtctctctg 720

gcggcggatc ccgttgaaga gatcaaagtg ggcttcgata ttctcaagtc gctgcgtatt 780

cgctctcgcg ggatcaactt tattgcctgc ccgacctgtt cacgtcagga gtttgacgtt 840

atcggtaccg ttaacgcgct ggagcagcgc ctggaagata tcattacgcc gatggatatt 900

tcgatcattg gctgcgtggt aaacggtccc ggcgaggcgc tggtttccac cctcggcgta 960

accggcggca ataagaaaag cggcctgtac gaagacggcg tacgtaaaga caggctggat 1020

aacgacgata tgatcgatca gctggaagcg cgtattcgcg ctaaagcatc gatgctggat 1080

gaggcgcgtc gtatcgatat ccagcaggtt gaagcgaaat aa 1122

<210> 33

<211> 876

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 33

atgagccata ttcaacggga aacgtcttgc tccaggccgc gattaaattc caacatggat 60

gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc 120

tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc 180

gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct 240

cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg 300

atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt 360

gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct 420

tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg 480

gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa 540

gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca 600

cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc 660

ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct 720

tatggtattg ataatcctga tatgaataaa 780

ttgcagtttc atttgatgct cgatgagttt ttctaataag cctgcctggt tctgcgtttc 840

ccgctcttta ataccctgac cggaggtgag caatga 876

<210> 34

<211> 1491

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 34

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gagcgtgttg 120

agtcatcctg actagctgag atgagggctc gccccctcgt cccgacactt ccagatcgcc 180

atagcgcaca gcgcctcgag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat 240

gactgtttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc 300

gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct 360

aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca 420

gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac 480

ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg 540

accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg 600

gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac 660

gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc 720

aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg 780

ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt 840

gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac 900

tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg 960

tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag 1020

cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa 1080

gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa 1140

ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc 1200

ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa 1260

actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat 1320

ggtacccgac cggtggtgaa tttaatctcg ctgacgtgta gacattccct tatccagacg 1380

ctgatcgccc atcatcgcgg ttctttagat ctctcggtcc gccctgatgg cggcaccttg 1440

ctgacgttac gcctgccggt acagcaggtt atcaccggag gcttaaaatg a 1491

<210> 35

<211> 1021

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 35

ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa 60

tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc 120

ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc 180

caggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 240

taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga 300

gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 360

actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 420

tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga 480

agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 540

gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca 600

ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 660

tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga 720

ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 780

aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 840

cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 900

tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagtttt 960

ctaataagcc ttgaccctac gattcccgct atttcattca ctgaccggag gttcaaaatg 1020

a 1021

<210> 36

<211> 1071

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 36

atgaagatag caacaatgaa aacaggtctg ggagcgttgg ctcttcttcc ctgatccttc 60

aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa tctctgatgt 120

tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac 180

agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc ccgtccgcgc 240

ttaaactcca acatggacgc tgatttatat gggtataaat gggctcgcga taatgtcggg 300

caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg 360

aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtccg tctcaactgg 420

ctgacggagt ttatgcctct cccgaccatc aagcatttta tccgtactcc tgatgatgcg 480

tggttactca ccaccgcgat tcctgggaaa acagccttcc aggtattaga agaatatcct 540

gattcaggtg aaaatattgt tgatgcgctg gccgtgttcc tgcgccggtt acattcgatt 600

660

cgcatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct 720

gttgaacaag tctggaaaga aatgcacaag ctcttgccat tctcaccggga ttcagtcgtc 780

actcatggtg atttctcact tgataacctt atttttgacg agggggaaatt aataggttgt 840

attgatgttg gacgggtcgg aatcgcagac cgttaccagg accttgccat tctttggaac 900

tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat 960

aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagtttt ctaataagcc 1020

tgtgaagggc tggacgtaaa cagccacggc gaaaacgcct acaacgcctg a 1071

<210> 37

<211> 1071

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 37

atgaccctga atatgatgct cgataacgcc gtacccgagg cgattgccgg ctgatccttc 60

aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa tctctgatgt 120

tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac 180

agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc ccgtccgcgc 240

ttaaactcca acatggacgc tgatttatat gggtataaat gggctcgcga taatgtcggg 300

caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg 360

aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtccg tctcaactgg 420

ctgacggagt ttatgcctct cccgaccatc aagcatttta tccgtactcc tgatgatgcg 480

tggttactca ccaccgcgat tcctgggaaa acagccttcc aggtattaga agaatatcct 540

gattcaggtg aaaatattgt tgatgcgctg gccgtgttcc tgcgccggtt acattcgatt 600

660

cgcatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct 720

gttgaacaag tctggaaaga aatgcacaag ctcttgccat tctcaccggga ttcagtcgtc 780

actcatggtg atttctcact tgataacctt atttttgacg agggggaaatt aataggttgt 840

attgatgttg gacgggtcgg aatcgcagac cgttaccagg accttgccat tctttggaac 900

tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat 960

aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagtttt ctaataagcc 1020

ttggttctgc gtttcccgct ctttaatacc ctgaccggag gtgagcaatg a 1071

<210> 38

<211> 426

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 38

atgaccctga atatgatgat ggatgccggc ggacatcatc gcgacaaaca atattaatac 60

cggcaaccac accggcaatt tacgagactg cgcaggcatc ctttctcccg tcaatttctg 120

tcaaataaag taaaagaggc agtctacttg aattaccccc ggctggttga gcgtttgttg 180

aaaaaaagta actgaaaaat ccgtagaata gcgccactct gatggttaat taacctattc 240

aattaagaat tatctggatg aatgtgccat taaatgcgca gcataatggt gcgttgtgcg 300

ggaaaactgc ttttttttga aagggttggt cagtagcgga aacaactcac ttcacacccc 360

gaagggggaa gttgcctgac cctacgattc ccgctatttc attcactgac cggaggttca 420

aaatga 426

<210> 39

<211> 446

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 39

atgaccctga atatgatgat ggatgccggc tcaccacggc gataaccata ggttttcggc 60

gtggccacat ccatggtgaa tcccactttt tccagcacgc gcgccacttc atcgggtctt 120

aaatacatag attttcctcg tcatctttcc aaagcctcgc caccttacat gactgagcat 180

ggaccgtgac tcagaaaatt ccacaaacga acctgaaagg cgtgattgcc gtctggcctt 240

aaaaattatg gtctaaacta aaatttacat cgaaaacgag ggaggatcct atgtttaaca 300

aaccgaatcg ccgtgacgta gatgaaggtg ttgaggatat taaccacgat gttaaccagc 360

tcgaactcac ttcacacccc gaagggggaa gttgcctgac cctacgattc ccgctatttc 420

attcactgac cggaggttca aaatga 446

<210> 40

<211> 452

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 40

atgaccctga atatgatgat ggatgccggc tgacgaggca ggttacatca ctggtgaaac 60

120

agggcgaaag gcctcaaaat agcgtaaaat cgtggtaaga actgccggga tttagttgca 180

aatttttcaa cattttatac actacgaaaa ccatcgcgaa agcgagtttt gataggaaat 240

ttaagagtat gagcactatc gaagaacgcg ttaagaaaat tatcggcgaa cagctgggcg 300

ttaagcagga agaagttacc aacaatgctt ccttcgttga agacctgggc gctgattctc 360

ttgacaccga actcacttca caccccgaag ggggaagttg cctgacccta cgattcccgc 420

tattcattc actgaccgga ggttcaaaat ga 452

<210> 41

<211> 461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 41

atgaccctga atatgatgat ggatgccggc cgtcctgtaa taataaccgg acaattcgga 60

ctgattaaaa aagcgccctt gtggcgcttt ttttatattc ccgcctccat ttaaaataaa 120

aaatccaatc ggatttcact atttaaactg gccattatct aagatgaatc cgatggaagc 180

240

cgatcaaatt taagtggatt cccatcaaaa aaatattctc aacctaaaaa agtttgtgta 300

atacttgtaa cgctacatgg agattaactc aatctagagg gtattaataa tgaatcgtac 360

taaactggta ctgggcgcaa ctcacttcac accccgaagg gggaagttgc ctgaccctac 420

gattcccgct atttcattca ctgaccggag gttcaaaatg a 461

<210> 42

<211> 463

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 42

atgaccctga atatgatgat ggatgccggc atattgacac catgacgcgc gtaatgctga 60

ttggttctgt gacgctggta atgattgtcg aaattctgaa cagtgccatc gaagccgtag 120

180

cggcggtgct gatgtccatc ctgctggcga tgtttacctg gatcgcatta ctctggtcac 240

attttcgata acgcttccag aattcgataa cgccctggtt ttttgcttaa atttggttcc 300

aaaatcgcct ttagctgtat atactcacag cataactgta tatacaccca gggggcggga 360

tgaaagcatt aacggccagg aactcacttc acaccccgaa gggggaagtt gcctgaccct 420

acgattcccg ctatttcatt cactgaccgg aggttcaaaa tga 463

<210> 43

<211> 428

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 43

atgaccctga atatgatgat ggatgccggc atcatattgc gctccctggt tatcatttgt 60

tactaaatga aatgttataa tataacaatt ataaatacca catcgctttc aattcaccag 120

ccaaatgaga ggagcgccgt ctgacatagc cagcgctata aaacatagca ttatctatat 180

gtttatgatt aataactgat ttttgcgttt tggatttggc tgtggcatcc ttgccgctct 240

tttcgcagcg tctgcgtttt tgccctccgg tcagggcatt taagggtcag caatgagttt 300

ttacgcaatt acgattcttg ccttcggcat gtcgatggat gctttaactc acttcacacc 360

ccgaaggggg aagttgcctg accctacgat tcccgctatt tcattcactg accggaggtt 420

caaaatga 428

<210> 44

<211> 452

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 44

atgaccctga atatgatgat ggatgccggc cgcgtcaggt tgaacgtaaa aaagtcggtc 60

tgcgcaaagc acgtcgtcgt ccgcagttct ccaaacgtta attggtttct gcttcggcag 120

aacgattggc gaaaaaaccc ggtgcgaacc gggttttttt atggataaag atcgtgttat 180

ccacagcaat ccattgatta tctcttcttt ttcagcattt ccagaatccc ctcaccacaa 240

agcccgcaaa atctggtaaa ctatcatcca attttctgcc caaatggctg ggattgttca 300

gccttacaac gagagtgaca gtacgcgcgg gtagttaact caacatctga 360

ccggtcgata actcacttca caccccgaag ggggaagttg cctgacccta cgattcccgc 420

tattcattc actgaccgga ggttcaaaat ga 452

<210> 45

<211> 410

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 45

atgaccctga atatgatgat ggatgccggc cctgtatgaa gatggcgtgc gcaaagatcg 60

cctggataac agcgatatga ttagccagct tgaagcccgc attcgcgcga aagcgtcaat 120

gctggacgaa gcgcgtcgta tcgatgtgca acaggtagaa aaataaggtt gctgggaagc 180

ggcaggcttc ccgtgtatga tgaacccgcc cggcgcgacc cgttgttcgt cgcggccccg 240

agggttcatt ttttgtatta ataaagagaa taaacgtggc aaaaaatatt caagccattc 300

gcggcatgaa cgattatctg cctggcgaac tcacttcaca ccccgaaggg ggaagttgcc 360

tgaccctacg attcccgcta tttcattcac tgaccgggagg ttcaaaatga 410

<210> 46

<211> 1071

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 46

atgaaaaaga ttgatgcgat tattaaacct ttcaaactgg atgacgtgcg ctgatccttc 60

aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa tctctgatgt 120

tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac 180

agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc ccgtccgcgc 240

ttaaactcca acatggacgc tgatttatat gggtataaat gggctcgcga taatgtcggg 300

caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg 360

aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtccg tctcaactgg 420

ctgacggagt ttatgcctct cccgaccatc aagcatttta tccgtactcc tgatgatgcg 480

tggttactca ccaccgcgat tcctgggaaa acagccttcc aggtattaga agaatatcct 540

gattcaggtg aaaatattgt tgatgcgctg gccgtgttcc tgcgccggtt acattcgatt 600

660

cgcatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct 720

gttgaacaag tctggaaaga aatgcacaag ctcttgccat tctcaccggga ttcagtcgtc 780

actcatggtg atttctcact tgataacctt atttttgacg agggggaaatt aataggttgt 840

attgatgttg gacgggtcgg aatcgcagac cgttaccagg accttgccat tctttggaac 900

tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat 960

aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagtttt ctaataagcc 1020

tcgcgcgtga ttcgtatccg caccggcgaa gaagacgacg cggcgattta a 1071

<210> 47

<211> 1295

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 47

atgaccatga acctgatgac ggatgtcgtc tcagccaccg ggatcgccgg gttgctttca 60

cgacaacacc cgacgctgtt ttttacacta attgaacagg cccccgtggc gatcacgctg 120

acggataccg ctgcccgcat tgtctatgcc aacccgggcg tgttgagtca tcctgactag 180

ctgagatgag ggctcgcctg atccttcaac tcagcaaaag ttcgattat tcaacaaagc 240

cacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga 300

acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa 360

cgggaaacgt cttgctccag gccgcgatta aattccaaca tggatgctga tttatatggg 420

tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg 480

aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt 540

acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag 600

cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca 660

gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca 720

gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc 780

gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat 840

tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataagctt 900

ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt 960

tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga 1020

taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa 1080

1140

atgctcgatg agtttttcta ataagcctga ccggtggtga atttaatctc gctgacgtgt 1200

1260

cgctctttta tgaactgacc ggaggcccaa gatga 1295

<210> 48

<211> 1491

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 48

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gagcgtgttg 120

agtcatcctg actagctgag atgagggctc gccccctcgt cccgacactt ccagatcgcc 180

atagcgcaca gcgcctcgag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat 240

gactgtttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc 300

gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct 360

aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca 420

gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac 480

ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg 540

accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg 600

gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac 660

gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc 720

aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg 780

ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt 840

gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac 900

tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg 960

tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag 1020

cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa 1080

gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa 1140

ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc 1200

ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa 1260

actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat 1320

ggtacccgac cggtggtgaa tttaatctcg ctgacgtgta gacattccct tatccagacg 1380

ctgatcgccc atcatcgcgg ttctttagat ctctcggtcc gccctgatgg cggcaccttg 1440

ctgacgttac gcctgccggt acagcaggtt atcaccggag gcttaaaatg a 1491

<210> 49

<211> 1021

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 49

ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa 60

tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc 120

ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc 180

caggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 240

taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga 300

gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 360

actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 420

tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga 480

agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 540

gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca 600

ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 660

tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga 720

ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 780

aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 840

cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 900

tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagtttt 960

ctaataagcc ttgaccctac gattcccgct atttcattca ctgaccggag gttcaaaatg 1020

a 1021

<210> 50

<211> 1071

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 50

atgaccctga atatgatgct cgataacgcc gtacccgagg cgattgccgg ctgatccttc 60

aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa tctctgatgt 120

tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac 180

agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc ccgtccgcgc 240

ttaaactcca acatggacgc tgatttatat gggtataaat gggctcgcga taatgtcggg 300

caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg 360

aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtccg tctcaactgg 420

ctgacggagt ttatgcctct cccgaccatc aagcatttta tccgtactcc tgatgatgcg 480

tggttactca ccaccgcgat tcctgggaaa acagccttcc aggtattaga agaatatcct 540

gattcaggtg aaaatattgt tgatgcgctg gccgtgttcc tgcgccggtt acattcgatt 600

660

cgcatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct 720

gttgaacaag tctggaaaga aatgcacaag ctcttgccat tctcaccggga ttcagtcgtc 780

actcatggtg atttctcact tgataacctt atttttgacg agggggaaatt aataggttgt 840

attgatgttg gacgggtcgg aatcgcagac cgttaccagg accttgccat tctttggaac 900

tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat 960

aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagtttt ctaataagcc 1020

ttggttctgc gtttcccgct ctttaatacc ctgaccggag gtgagcaatg a 1071

<210> 51

<211> 461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 51

atgaccctga atatgatgat ggatgccggc cgtcctgtaa taataaccgg acaattcgga 60

ctgattaaaa aagcgccctt gtggcgcttt ttttatattc ccgcctccat ttaaaataaa 120

aaatccaatc ggatttcact atttaaactg gccattatct aagatgaatc cgatggaagc 180

240

cgatcaaatt taagtggatt cccatcaaaa aaatattctc aacctaaaaa agtttgtgta 300

atacttgtaa cgctacatgg agattaactc aatctagagg gtattaataa tgaatcgtac 360

taaactggta ctgggcgcaa ctcacttcac accccgaagg gggaagttgc ctgaccctac 420

gattcccgct atttcattca ctgaccggag gttcaaaatg a 461

<210> 52

<211> 426

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 52

atgaccctga atatgatgat ggatgccggc ggacatcatc gcgacaaaca atattaatac 60

cggcaaccac accggcaatt tacgagactg cgcaggcatc ctttctcccg tcaatttctg 120

tcaaataaag taaaagaggc agtctacttg aattaccccc ggctggttga gcgtttgttg 180

aaaaaaagta actgaaaaat ccgtagaata gcgccactct gatggttaat taacctattc 240

aattaagaat tatctggatg aatgtgccat taaatgcgca gcataatggt gcgttgtgcg 300

ggaaaactgc ttttttttga aagggttggt cagtagcgga aacaactcac ttcacacccc 360

gaagggggaa gttgcctgac cctacgattc ccgctatttc attcactgac cggaggttca 420

aaatga 426

<210> 53

<211> 452

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 53

atgaccctga atatgatgat ggatgccggc tgacgaggca ggttacatca ctggtgaaac 60

120

agggcgaaag gcctcaaaat agcgtaaaat cgtggtaaga actgccggga tttagttgca 180

aatttttcaa cattttatac actacgaaaa ccatcgcgaa agcgagtttt gataggaaat 240

ttaagagtat gagcactatc gaagaacgcg ttaagaaaat tatcggcgaa cagctgggcg 300

ttaagcagga agaagttacc aacaatgctt ccttcgttga agacctgggc gctgattctc 360

ttgacaccga actcacttca caccccgaag ggggaagttg cctgacccta cgattcccgc 420

tattcattc actgaccgga ggttcaaaat ga 452

<210> 54

<211> 426

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 54

atgaccctga atatgatgat ggatgccggc ggacatcatc gcgacaaaca atattaatac 60

cggcaaccac accggcaatt tacgagactg cgcaggcatc ctttctcccg tcaatttctg 120

tcaaataaag taaaagaggc agtctacttg aattaccccc ggctggttga gcgtttgttg 180

aaaaaaagta actgaaaaat ccgtagaata gcgccactct gatggttaat taacctattc 240

aattaagaat tatctggatg aatgtgccat taaatgcgca gcataatggt gcgttgtgcg 300

ggaaaactgc ttttttttga aagggttggt cagtagcgga aacaactcac ttcacacccc 360

gaagggggaa gttgcctgac cctacgattc ccgctatttc attcactgac cggaggttca 420

aaatga 426

<210> 55

<211> 461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 55

atgaccctga atatgatgat ggatgccggc cgtcctgtaa taataaccgg acaattcgga 60

ctgattaaaa aagcgccctt gtggcgcttt ttttatattc ccgcctccat ttaaaataaa 120

aaatccaatc ggatttcact atttaaactg gccattatct aagatgaatc cgatggaagc 180

240

cgatcaaatt taagtggatt cccatcaaaa aaatattctc aacctaaaaa agtttgtgta 300

atacttgtaa cgctacatgg agattaactc aatctagagg gtattaataa tgaatcgtac 360

taaactggta ctgggcgcaa ctcacttcac accccgaagg gggaagttgc ctgaccctac 420

gattcccgct atttcattca ctgaccggag gttcaaaatg a 461

<210> 56

<211> 1491

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 56

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gagcgtgttg 120

agtcatcctg actagctgag atgagggctc gccccctcgt cccgacactt ccagatcgcc 180

atagcgcaca gcgcctcgag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat 240

gactgtttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc 300

gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct 360

aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca 420

gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac 480

ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg 540

accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg 600

gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac 660

gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc 720

aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg 780

ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt 840

gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac 900

tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg 960

tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag 1020

cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa 1080

gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa 1140

ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc 1200

ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa 1260

actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat 1320

ggtacccgac cggtggtgaa tttaatctcg ctgacgtgta gacattccct tatccagacg 1380

ctgatcgccc atcatcgcgg ttctttagat ctctcggtcc gccctgatgg cggcaccttg 1440

ctgacgttac gcctgccggt acagcaggtt atcaccggag gcttaaaatg a 1491

<210> 57

<211> 1491

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 57

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gagcgtgttg 120

agtcatcctg actagctgag atgagggctc gccccctcgt cccgacactt ccagatcgcc 180

atagcgcaca gcgcctcgag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat 240

gactgtttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc 300

gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct 360

aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca 420

gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac 480

ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg 540

accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg 600

gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac 660

gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc 720

aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg 780

ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt 840

gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac 900

tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg 960

tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag 1020

cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa 1080

gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa 1140

ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc 1200

ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa 1260

actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat 1320

ggtacccgac cggtggtgaa tttaatctcg ctgacgtgta gacattccct tatccagacg 1380

ctgatcgccc atcatcgcgg ttctttagat ctctcggtcc gccctgatgg cggcaccttg 1440

ctgacgttac gcctgccggt acagcaggtt atcaccggag gcttaaaatg a 1491

<210> 58

<211> 1021

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 58

ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa 60

tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc 120

ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc 180

caggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 240

taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga 300

gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 360

actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 420

tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga 480

agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 540

gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca 600

ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 660

tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga 720

ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 780

aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 840

cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 900

tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagtttt 960

ctaataagcc ttgaccctac gattcccgct atttcattca ctgaccggag gttcaaaatg 1020

a 1021

<210> 59

<211> 1491

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 59

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gagcgtgttg 120

agtcatcctg actagctgag atgagggctc gccccctcgt cccgacactt ccagatcgcc 180

atagcgcaca gcgcctcgag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat 240

gactgtttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc 300

gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct 360

aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca 420

gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac 480

ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg 540

accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg 600

gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac 660

gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc 720

aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg 780

ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt 840

gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac 900

tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg 960

tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag 1020

cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa 1080

gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa 1140

ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc 1200

ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa 1260

actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat 1320

ggtacccgac cggtggtgaa tttaatctcg ctgacgtgta gacattccct tatccagacg 1380

ctgatcgccc atcatcgcgg ttctttagat ctctcggtcc gccctgatgg cggcaccttg 1440

ctgacgttac gcctgccggt acagcaggtt atcaccggag gcttaaaatg a 1491

<210> 60

<211> 1491

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 60

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gagcgtgttg 120

agtcatcctg actagctgag atgagggctc gccccctcgt cccgacactt ccagatcgcc 180

atagcgcaca gcgcctcgag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat 240

gactgtttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc 300

gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct 360

aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca 420

gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac 480

ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg 540

accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg 600

gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac 660

gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc 720

aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg 780

ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt 840

gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac 900

tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg 960

tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag 1020

cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa 1080

gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa 1140

ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc 1200

ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa 1260

actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat 1320

ggtacccgac cggtggtgaa tttaatctcg ctgacgtgta gacattccct tatccagacg 1380

ctgatcgccc atcatcgcgg ttctttagat ctctcggtcc gccctgatgg cggcaccttg 1440

ctgacgttac gcctgccggt acagcaggtt atcaccggag gcttaaaatg a 1491

<210> 61

<211> 1563

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<400> 61

atgtttaacg atctgattgg cgatgatgaa acggattcgc cggaagatgc gctttctgag 60

agctggcgcg aattgtggca ggatgcgttg caggaggagg attccacgcc cgtgctggcg 120

catctctcag aggacgatcg ccgccgcgtg gtggcgctga ttgccgattt tcgcaaagag 180

ttggataaac gcaccattgg cccgcgaggg cggcaggtac tcgatcactt aatgccgcat 240

ctgctcagcg atgtatgctc gcgcgacgat gcgccagtac cgctgtcacg cctgacgccg 300

ctgctcaccg gaattattac ccgcaccact taccttgagc tgctaagtga atttcccggc 360

gcactgaaac acctcatttc cctgtgtgcc gcgtcgccga tggttgccag tcagctggcg 420

cgctacccga tcctgcttga tgaattgctc gacccgaata cgctctatca accgacggcg 480

atgaatgcct atcgcgatga gctgcgccaa tacctgctgc gcgtgccgga agatgatgaa 540

gagcaacagc ttgaggcgct gcggcagttt aagcaggcgc agttgctgcg cgtggcggcg 600

gcggatattg ccggtacgtt gccagtaatg aaagtgagcg atcacttaac ctggctggcg 660

gaagcgatta ttgatgcggt ggtgcagcaa gcctgggggc agatggtggc gcgttatggc 720

cagccaacgc atctgcacga tcgcgaaggg cgcggttttg cggtggtcgg ttatggcaag 780

ctgggcggct gggagctggg ttacagctcc gatctggatc tggtattcct gcacgactgc 840

ccgatggatg tgatgaccga tggcgagcgt gaaatcgatg gtcgccagtt ctatttgcgt 900

ctcgcgcagc gcgtgatgca cctgtttagc acgcgcacgt cgtccggcat cctttatgaa 960

gttgatgcgc gtctgcgtcc atctggcgct gcggggatgc tggtcactac tacggaatcg 1020

ttcgccgatt accagcaaaa cgaagcctgg acgtgggaac atcaggcgct ggcccgtgcg 1080

cgcgtggtgt acggcgatcc gcaactgacc gccgaatttg acgccattcg ccgcgatatt 1140

ctgatgacgc ctcgcgacgg cgcaacgctg caaaccgacg tgcgagaaat gcgcgagaaa 1200

atgcgtgccc atcttggcaa caagcataaa gaccgcttcg atctgaaagc cgatgaaggc 1260

ggtatcaccg acatcgagtt tatcgcccaa tatctggtgc tgcgctttgc ccatgacaag 1320

ccgaaactga cgcgctggtc ggataatgtg cgcattctcg aagggctggc gcaaaacggc 1380

atcatggagg agcaggaagc gcaggcattg acgctggcgt acaccacatt gcgtgatgag 1440

ctgcaccacc tggcgctgca agagttgccg ggacatgtgg cgctctcctg ttttgtcgcc 1500

1560 gagcgtgcgc ttattaaaac cagctgggac

taa 1563

<210> 62

<211> 1536

<212> DNA

<213> Kosakonia sacchari

<220>

<223> rDNA 16S - contig 5, strain CI006

<400> 62

ttgaagagtt tgatcatggc tcagattgaa cgctggcggc aggcctaaca catgcaagtc 60

gaacggtagc acagagagct tgctctcggg tgacgagtgg cggacgggtg agtaatgtct 120

gggaaactgc ctgatggagg gggataacta ctggaaacgg tagctaatac cgcataacgt 180

cgcaagacca aagaggggga ccttcgggcc tcttgccatc agatgtgccc agatgggatt 240

agctagtagg tggggtaacg gctcacctag gcgacgatcc ctagctggtc tgagaggatg 300

accagccaca ctggaactga gacacggtcc agactcctac gggaggcagc agtggggaat 360

attgcacaat gggcgcaagc ctgatgcagc catgccgcgt gtgtgaagaa ggccttcggg 420

ttgtaaagca ctttcagcgg ggaggaaggg agtaaggtta ataaccttat tcattgacgt 480

tacccgcaga agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc 540

aagcgttaat cggaattact gggcgtaaag cgcacgcagg cggtctgtca agtcggatgt 600

gaaatccccg ggctcaacct gggaactgca tccgaaactg gcaggcttga gtctcgtaga 660

gggaggtaga attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg 720

cgaaggcggc ctcctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag 780

gattagatac cctggtagtc cacgccgtaa acgatgtcta tttggaggtt gtgcccttga 840

ggcgtggctt ccggagctaa cgcgttaaat agaccgcctg gggagtacgg ccgcaaggtt 900

aaaactcaaa tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat 960

gcaacgcgaa gaaccttacc tggtcttgac atccacagaa ctttccagag atggattggt 1020

gccttcggga actgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt 1080

1140

actcaaagga gactgccagt gataaactgg aggaaggtgg ggatgacgtc aagtcatcat 1200

ggcccttacg accagggcta cacacgtgct acaatggcgc atacaaagag aagcgacctc 1260

gcgagagtaa gcggacctca taaagtgcgt cgtagtccgg attggagtct gcaactcgac 1320

1380

ggccttgtac acaccgcccg tcacaccatg ggagtgggtt gcaaaagaag taggtagctt 1440

aaccttcggg agggcgctta ccactttgtg attcatgact ggggtgaagt cgtaacaagg 1500

taaccgtagg ggaacctgcg gttggatcac ctcctt 1536

<210> 63

<211> 1537

<212> DNA

<213> Kosakonia sacchari

<220>

<223> rDNA 16S - contig 8, strain CI006

<220>

<221> modified base

<222> (450)..(450)

<223> a, c, g or t

<220>

<221> modified base

<222> (452)..(452)

<223> a, c, g or t

<220>

<221> modified base

<222> (455)..(455)

<223> a, c, g or t

<220>

<221> modified base

<222> (473)..(473)

<223> a, c, g or t

<400> 63

ttgaagagtt tgatcatggc tcagattgaa cgctggcggc aggcctaaca catgcaagtc 60

gaacggtagc acagagagct tgctctcggg tgacgagtgg cggacgggtg agtaatgtct 120

gggaaactgc ctgatggagg gggataacta ctggaaacgg tagctaatac cgcataacgt 180

cgcaagacca aagaggggga ccttcgggcc tcttgccatc agatgtgccc agatgggatt 240

agctagtagg tggggtaacg gctcacctag gcgacgatcc ctagctggtc tgagaggatg 300

accagccaca ctggaactga gacacggtcc agactcctac gggaggcagc agtggggaat 360

attgcacaat gggcgcaagc ctgatgcagc catgccgcgt gtgtgaagaa ggccttcggg 420

ttgtaaagca ctttcagcgg ggaggaaggn antanggtta ataacctgtg ttnattgacg 480

ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat acggagggtg 540

caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtctgtc aagtcggatg 600

tgaaatcccc gggctcaacc tgggaactgc atccgaaact ggcaggcttg agtctcgtag 660

agggaggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg aataccggtg 720

gcgaaggcgg ccctcctggac gaagactgac gctcaggtgc gaaagcgtgg ggagcaaaca 780

ggattagata ccctggtagt ccacgccgta aacgatgtct atttggaggt tgtgcccttg 840

aggcgtggct tccggagcta acgcgttaaa tagaccgcct ggggagtacg gccgcaaggt 900

taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga 960

tgcaacgcga agaaccttac ctggtcttga catccacaga acttagcaga gatgctttgg 1020

tgccttcggg aactgtgaga caggtgctgc atggctgtcg tcagctcgtg ttgtgaaatg 1080

ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg ttaggccggg 1140

aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt caagtcatca 1200

tggcccttac gaccaggggct acacacgtgc tacaatggcg catacaaaga gaagcgacct 1260

1320 cgcgagagta agcggacctc ataaagtgcg tcgtagtccg

ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga atacgttccc 1380

gggccttgta cacaccgcc gtcacaccat gggagtgggt tgcaaaagaa gtaggtagct 1440

taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag tcgtaacaag 1500

gtaaccgtag gggaacctgc ggttggatca ccttcctt 1537

<210> 64

<211> 1540

<212> DNA

<213> view of Rahnella

<220>

<223> rDNA 16S, strain CI019

<220>

<221> modified base

<222> (70)..(70)

<223> a, c, g or t

<220>

<221> modified base

<222> (267)..(267)

<223> a, c, g or t

<220>

<221> modified base

<222> (455)..(455)

<223> a, c, g or t

<220>

<221> modified base

<222> (458)..(458)

<223> a, c, g or t

<220>

<221> modified base

<222> (473)..(473)

<223> a, c, g or t

<220>

<221> modified base

<222> (1135)..(1135)

<223> a, c, g or t

<400> 64

attgaagagt ttgatcatgg ctcagattga acgctggcgg caggcctaac acatgcaagt 60

cgagcggcan cgggaagtag cttgctactt tgccggcgag cggcggacgg gtgagtaatg 120

tctgggaaac tgcctgatgg agggggataa ctactggaaa cggtagctaa taccgcatga 180

cctcgaaaga gcaaagtggg ggatcttcgg acctcacgcc atcggatgtg cccagatggg 240

attagctagt aggtgaggta atggctnacc taggcgacga tccctagctg gtctgagagg 300

atgaccagcc acactggaac tgagacacgg tccagactcc tacgggaggc agcagtgggg 360

aatattgcac aatgggcgca agcctgatgc agccatgccg cgtgtgtgaa gaaggcctta 420

gggttgtaaa gcactttcag cgaggaggaa ggcancanac ttaatacgtg tgntgattga 480

cgttactcgc agaagaagca ccggctaact ccgtgccagc agccgcggta atacggaggg 540

tgcaagcgtt aatcggaatt actgggcgta aagcgcacgc aggcggtttg ttaagtcaga 600

tgtgaaatcc ccgagcttaa cttgggaact gcatttgaaa ctggcaagct agagtcttgt 660

agaggggggt agaattccag gtgtagcggt gaaatgcgta gagatctgga ggaataccgg 720

tggcgaaggc ggccccctgg acaaagactg acgctcaggt gcgaaagcgt ggggagcaaa 780

caggattaga taccctggta gtccacgctg taaacgatgt cgacttggag gttgtgccct 840

tgaggcgtgg cttccggagc taacgcgtta agtcgaccgc ctggggagta cggccgcaag 900

gttaaaactc aaatgaattg acggggggccc gcacaagcgg tggagcatgt ggtttaattc 960

gatgcaacgc gaagaacctt acctactctt gacatccaga gaatttgcca gagatggcga 1020

agtgccttcg ggaactctga gacaggtgct gcatggctgt cgtcagctcg tgttgtgaaa 1080

1140

gggaactcaa aggagactgc cggtgataaa ccggaggaag gtggggatga cgtcaagtca 1200

tcatggccct tacgagtagg gctacacacg tgctacaatg gcatatacaa agagaagcga 1260

actcgcgaga gcaagcggac ctcataaagt atgtcgtagt ccggattgga gtctgcaact 1320

cgactccatg tgaatacgtt 1380

cccgggcctt gtacacaccg cccgtcacac catgggagtg ggttgcaaaa gaagtaggta 1440

gcttaacctt cgggagggcg cttaccactt tgtgattcat gactggggtg aagtcgtaac 1500

aaggtaaccg taggggaacc tgcggttggga tcacctcctt 1540

<210> 65

<211> 882

<212> DNA

<213> Kosakonia sacchari

<220>

<223> nifH strain CI006

<400> 65

atgaccatgc gtcaatgcgc catttacggc aaaggtggga tcggcaaatc gaccaccaca 60

cagaacctgg tcgccgcgct ggcggagatg ggtaaaaaag tcatgattgt cggctgtgac 120

ccgaaagccg attccacgcg tttgatcctg catgcgaaag cgcagaacac cattatggag 180

atggctgctg aagtcggctc cgtggaagac ctggagttag aagacgtgct gcaaatcggt 240

tacggcggcg tgcgctgcgc agagtccggc ggcccggagc caggcgtggg ctgtgccggt 300

cgcggggtga tcaccgcgat taacttcctc gaagaagaag gcgcttacgt gccggatctc 360

gtggttgcg gtggtttcgc catgccgatt 420

cgtgaaaaca aagcgcagga gatctacatc gtttgctctg gcgaaatgat ggcgatgtac 480

gccgccaaca acatctccaa aggcatcgtg aaatacgcca aatccggtaa agtgcgcctc 540

ggcgggctga tttgtaactc gcgccagacc gaccgtgaag atgaactgat cattgcgctg 600

gcagaaaaac tcggcacgca gatgatccac tttgttcccc gcgacaacat tgtgcagcgt 660

gcggaaatcc gccgtatgac ggttatcgaa tatgacccga cctgcaatca ggcgaacgaa 720

tatcgcagcc ttgccagcaa aatcgtcaac aacaccaaaa tggtggtgcc caccccctgc 780

accatggatg aactggaaga actgctgatg gagttcggca ttatggatgt ggaagacacc 840

agcatcattg gtaaaaccgc cgccgaagaa aacgccgtct ga 882

<210> 66

<211> 1449

<212> DNA

<213> Kosakonia sacchari

<220>

<223> NifD strain CI006

<400> 66

atgagcaatg caacaggcga acgcaacctg gagataatcg agcaggtgct cgaggttttc 60

ccggagaaga cgcgcaaaga acgcagaaaa cacatgatgg tgacggaccc ggagcaggaa 120

agcgtcggta agtgcatcat ctctaaccgc aaatcgcagc caggcgtgat gaccgtgcgc 180

ggctgctcgt atgccggttc gaaaggggtg gtatttgggc caatcaagga tatggcgcat 240

atctcgcatg gcccaatcgg ctgcggccaa tactcccgcg ccgggcggcg gaactactac 300

accggcgtca gcggcgtgga cagcttcggc acgctcaact tcacctccga ttttcaggag 360

cgcgacatcg tgtttggcgg cgataaaaag ctcgccaaac tgattgaaga gctggaagag 420

ctgttcccgc tgaccaaagg catttcgatt cagtcggaat gcccggtcgg cctgattggc 480

gatgacattg aggccgtcgc gaacgccagc cgcaaagcca tcaacaaacc ggttattccg 540

gtgcgttgcg aaggctttcg cggcgtgtcg caatccctcg gtcaccatat tgccaacgat 600

gtgatccgcg actgggtgct ggataaccgc gaaggcaaac cgttcgaatc caccccttac 660

gatgtggcga tcatcggcga ttacaacatc ggcggcgatg cctgggcttc gcgcattttg 720

ctcgaagaga tgggcttgcg ggtggtggca cagtggtctg gcgacggtac gctggtggag 780

atggaaaaca cgccgttcgt caaactgaac ctggtgcatt gttaccgctc aatgaactac 840

atctcgcgcc atatggagga gaagcacggt attccgtgga tggaatacaa cttctttggt 900

ccgacgaaaa tcgcggaatc gctgcgcaaa atcgccgacc agtttgacga caccattcgc 960

gccaacgccg aagcggtgat cgccagatac caggcgcaaa acgacgccat tatcgccaaa 1020

tatcgcccgc gtctggaggg gcgcaaagtg ctgctttata tgggcgggct gcgtccgcgc 1080

catgtgattg gcgcctatga agacctggga atggagatca tcgctgccgg ttatgagttc 1140

ggtcataacg atgattacga ccgcaccttg ccggatctga aagagggcac gctgctgttt 1200

gatgatgcca gcagttatga gctggaggcg ttcgtcaacg cgctgaaacc ggatctcatc 1260

ggttccggca tcaaagagaa gtacatcttt cagaaaatgg gcgtgccgtt tcgccagatg 1320

cactcctggg attactccgg cccgtaccac ggctatgacg gcttcgccat cttcgcccgc 1380

gatatggata tgacgctcaa caaccccgcg tggggccagt tgaccgcgcc gtggctgaaa 1440

tccgcctga 1449

<210> 67

<211> 1563

<212> DNA

<213> Kosakonia sacchari

<220>

<223> nifK, strain CI006

<400> 67

atgagccaga ctgctgagaa aatacagaat tgccatcccc tgtttgaaca ggatgcttac 60

cagacgctgt ttgccggtaa acgggcactc gaagaggcgc actcgccgga gcgggtgcag 120

180

ctgactatcg acccggcaaa agcctgccag ccgctgggcg cggtgctctg ttcgctgggg 240

tttgccaata ccctaccgta tgtgcacggt tcacaggggtt gcgtggccta tttccgcacg 300

360

gcggcggtgt tcggcgggaa taacaacctc aacaccggct tacaaaacgc cagcgcgctg 420

tataaaccgg agattatcgc cgtctctacc acctgtatgg cggaagtgat cggtgatgat 480

ttgcaggcct ttatcgccaa cgccaaaaaa gatggttttc tcgatgccgc catccccgtg 540

ccctacgcgc acacccccag ttttatcggc agccatatca ccggctggga taacatgttt 600

gaaggttttg cccggacctt tacggcagac catgaagctc agcccggcaa actttcacgc 660

atcaacctgg tgaccgggtt tgaaacctat ctcggcaatt tccgcgtgct gaaacgcatg 720

atggaacaaa tggaggtgcc ggcgagtgtg ctctccgatc cgtcggaagt gctggatact 780

cccgccaacg ggcattacca gatgtacgcg ggcgggacga cgcagcaaga gatgcgcgag 840

gcgccggatg ctatcgacac cctgttgctg cagccctggc aactggtgaa aagcaaaaaa 900

gtggtgcagg agatgtggaa tcagcccgcc accgaggttt ctgttcccgt tgggctggca 960

ggaacagacg aactgttgat ggcgattagc cagttaaccg gcaaggccat tcccgattca 1020

ctggcgctgg agcgcgggcg gctggtcgat atgatgctcg attcccacac ctggttgcac 1080

ggtaaaaaat tcggcctgtt tggcgatccg gattttgtca tgggattgac ccgtttcctg 1140

ctggagctgg gctgcgaacc gaccgttatc ctctgccaca acggtaacaa acgctggcag 1200

1260

aactgcgatt tgtggcattt ccgctcgctg atgtttaccc gccagccgga ttttatgatt 1320

ggcaactcgt acggcaagtt cattcagcgc gacaccttag ccaaaggcga gcagtttgaa 1380

gttccgctga tccgcctcgg ttttcccctg ttcgaccgcc accatctgca ccgccagacc 1440

acctggggct acgagggcgc catgagcatt ctcactaccc ttgtgaatgc ggtactggag 1500

1560

taa 1563

<210> 68

<211> 1488

<212> DNA

<213> Kosakonia sacchari

<220>

<223> nifL strain CI006

<400> 68

atgaccctga atatgatgat ggatgccggc gcgcccgagg caatcgccgg tgcgctttcg 60

120

actgatgccg acgcacgcat tgtctatgcc aacccggctt tctgccgcca gaccggctat 180

gaactagaag cgttgttgca gcaaaatccc cgcctgcttg caagtcgcca aacccccgg 240

gaaatctatc aggatatgtg gcacaccttg ttacaacgcc gaccgtggcg cgggcaattg 300

attaaccgcc accgcgacgg cagcctgtat ctggtcgaga tcgatatcac cccggtgatt 360

aacccgtttg gcgaactgga acactacctg gcaatgcagc gcgatatcag cgccagttat 420

gcgctggagc agcggttgcg caatcacatg acgctgaccg aagcggtgct gaataacatt 480

ccggcggcgg tggttgtagt ggatgaacgc gatcatgtgg ttatggataa ccttgcctac 540

aaaacgttct gtgccgactg cggcggaaaa gagctcctga gcgaactcaa tttttcagcc 600

660

tggttgtcgg tgacctgctg ggcgctgccg ggcgtcagcg aagaagccag tcgctacttt 720

attgataaca ggctgacgcg cacgctggtg gtgatcaccg acgacaccca acaacgccag 780

cagcaggaac agggccgact tgaccgcctt aaacagcaga tgaccaacgg caaactactg 840

gcagcgatcc gcgaagcgct tgacgccgcg ctgatccagc ttaactgccc catcaatatg 900

ctggcggcgg cgcgacgttt aaacggcagt gataacaaca atgtggcgct cgacgccgcg 960

tggcgcgaag gtgaagaggc gatggcgcgg ctgaaacgtt gccgcccgtc gctggaactg 1020

gaaagtgcgg ccgtctggcc gctgcaaccc ttttttgacg atctgcgcgc gctttatcac 1080

acccgctacg agcaggggaa aaatttgcag gtcacgctgg attcccatca tctggtggga 1140

tttggtcagc gtacgcaact gttagcctgc ctgagtctgt ggctcgatcg cacgctggat 1200

1260

ggctggctct ctttgtatat cactgacaat gtgccgctga tcccgctgcg cccacacccac 1320

1380 tcgccggatg cgcttaacgc tccgggaaaa ggcatggagc

gtggcacacc accacggcgc aatagaactc acttcacacc ccgaaggggg aagttgcctg 1440

accctacgat tcccgctatt tcattcactg accggaggtt caaaatga 1488

<210> 69

<211> 1575

<212> DNA

<213> Kosakonia sacchari

<220>

<223> nifA strain CI006

<400> 69

atgacccagc gaaccgagtc gggtaatacc gtctggcgct tcgatttgtc ccagcagttc 60

actgcgatgc agcgcataag cgtggtactc agccgggcga ccgaggtcga tcagacgctc 120

cagcaagtgc tgtgcgtatt gcacaatgac gccttttttgc agcacggcat gatctgtctg 180

tacgacagcc agcaggcgat tttgaatatt gaagcgttgc aggaagccga tcagcagtta 240

atccccggca gctcgcaaat ccgctatcgt ccgggcgaag ggctggtcgg gacggtgctt 300

tcgcagggcc aatcattagt gctggcgcgc gttgctgacg atcagcgctt tcttgaccgg 360

ctcgggttgt atgattacaa ccgccgttt atcgccgtgc cgctgatagg gccagatgcg 420

cagactttcg gtgtgctgac ggcacaaccc atggcgcgtt acgaagagcg attacccgcc 480

tgcacccgct ttctggaaac ggtcgctaac ctggtcgcgc aaaccgtgcg tttgatggca 540

ccaccggcag tgcgcccttc cccgcgcgcc gccataacac aggccgccag cccgaaatcc 600

tgcacggcct cacgcgcatt tggttttgaa aatatggtcg gtaacagtcc ggcgatgcgc 660

cagaccatgg agattatccg tcaggtttcg cgctgggaca ccaccgttct ggtacgcggc 720

gagagtggca ccggcaagga gctgattgcc aacgccatcc accaccattc gccgcgtgcc 780

ggtgcgccat ttgtgaaatt caactgtgcg gcgctgccgg acacactgct ggaaagcgaa 840

ttgttcggtc acgagaaagg ggcatttacc ggcgcggtac gccagcgtaa aggccgtttt 900

gagctggccg atggcggcac gctgtttctt gacgagatcg gcgagagtag cgcctcgttt 960

caggctaagc tgctgcgcat tttgcaggaa ggcgaaatgg aacgcgtcgg cggcgacgag 1020

acattgcaag tgaatgtgcg cattattgcc gcgacgaacc gcaatcttga agatgaagtc 1080

cggctggggc actttcgcga agatctctat tatcgcctga atgtgatgcc catcgccctg 1140

ccgccactac gcgaacgcca ggaggacatt gccgagctgg cgcactttct ggtgcgtaaa 1200

atcgcccata accagagccg tacgctgcgc attagcgagg gcgctatccg cctgctgatg 1260

agctacaact ggcccggtaa tgtgcgcgaa ctggaaaact gccttgagcg ctcagcggtg 1320

atgtcggaga acggtctgat cgatcgggat gtgattttgt ttaatcatcg cgaccagcca 1380

gccaaaccgc cagttatcag cgtctcgcat gatgataact ggctcgataa caaccttgac 1440

gagcgccagc ggctgattgc ggcgctggaa aaagcgggat gggtacaagc caaagccgcg 1500

cgcttgctgg ggatgacgcc gcgccaggtc gcctatcgta ttcagacgat ggatataacc 1560

ctgccaaggc tataa 1575

<210> 70

<211> 876

<212> DNA

<213> view of Rahnella

<220>

<223> nifH strain CI019

<400> 70

atggcaatgc gtcaatgtgc aatctacggg aaagggggta ttggtaaatc caccactacc 60

caaaaccttg tagcggctct ggccgaaatg aataagaagg tcatgatcgt cggctgtgac 120

cctaaggctg attcaacccg cctcattctg catgcgaaag cacagaacac catcatggaa 180

atggccgctg aagtgggctc cgtggaagat ctggagctgg aagatgtgat gcaaatcggc 240

tatggcggcg tgcgctgtgc ggaatcaggc ggccctgagc ctggtgtggg ttgtgccgga 300

cgcggggtga tcaccgccat caacttcctc gaagaagaag gcgcgtatgt gccggatctg 360

gatttcgtgt tttacgacgt attgggcgat gtggtctgtg gcggtttcgc gatgccaatt 420

cgcgaaaaca aagcgcagga aatctacatc gtatgctccg gtgaaatgat ggcgatgtat 480

gccgccaaca acatttccaa aggcatcgtg aaatacgcga aatcgggcaa agttcgcctg 540

gccgggctga tctgtaactc ccgccagacg gatcgcgaag atgaactgat catcgcgctg 600

gctgaaaaac ttggcacgca aatgatccac ttcgtgccgc gtgacaacat tgtgcaacgc 660

gctgaaatcc gccgcatgac ggtcatcgaa tacgacccga cttgtgcgca ggcagatcag 720

tatcgtgcac tggcgaacaa aatcgtcaac aacaccaaaa tggtggtgcc gacaccggtc 780

840

accatcgtcg gtcgtaccgc cgccgaagag gcgtga 876

<210> 71

<211> 1449

<212> DNA

<213> view of Rahnella

<220>

<223> nifD strain CI019

<400> 71

atgaccagtg aaacacgcga acgtaacgag gcattgatcc aggaagtgct ggagatcttc 60

cccgagaagg cgcttaaaga tcgtaagaaa cacatgatga ccaccgaccc ggcgatggaa 120

tctgtcggca agtgtattgt ctcaaaccgc aaatcacagc cgggcgtgat gaccgtgcga 180

ggctgcgctt acgccggttc caaaggcgtg gtctttggcc cgatcaaaga catggcgcat 240

atctccccg gcccggttgg ttgcggccag tattctcgtg ccggacgccg taactattac 300

accggctgga gcggcgtgaa cagctttggc accctcaact tcaccagtga ttttcaggaa 360

cgggacatcg tatttggcgg cgataaaaag ctcgacaaac tgatcgacga actggagatg 420

ttgttcccgc tgaccaaagg catttcggta cagtcggaat gtccggtcgg tctgatcggc 480

gatgacattt ctgccgtcgc caaagccagc agcgccaaaa tcggtaagcc ggtcgtgccg 540

gtacgctgcg aggggttccg cggtgtgtcg caatcgctcg gccatcacat tgctaacgat 600

gtcatccgcg actgggtgct ggataaccgc gaaggcaatg aatttgaaac cacgccttac 660

gacgtggcga ttatcggcga ctacaacatc ggcggtgacg cctgggcctc acgtattctg 720

ctcgaagaaa tggggctgcg tgtggtggcg cagtggtccg gcgacggcac gctggtggag 780

atggaaaaca ccccgaaagt cgcactcaat ctggtgcact gctaccgctc gatgaactac 840

atctcccgtc atatggaaga aaaacacggc attccgtgga tggaatacaa cttctttggc 900

ccgaccaaaa ttgcggaatc tctgcgcgaa atcgcggcgc gttttgacga taccatccgg 960

aaaaacgccg aagcggtgat tgaaaaatat caggcgcaaa cgcaggcggt gatcgacaaa 1020

taccgtccgc gtctggaagg caaaaaggtg ctgttgtatc tcggcggttt acgtccgcgc 1080

cacatcatcg gggcgtatga agatctggga atggaaatca tcggtaccgg ctatgaattc 1140

ggtcataacg atgattacga ccgcacctta ccgatgctca aagaaggcac gttgctgttc 1200

gatgacctga gcagttatga gctggaagcg ttcgttaaag cgctgaaacc ggatcttgtc 1260

gggtcaggca tcaaagaaaa atacattttc cagaaaatgg gcgtgccgtt ccgccagatg 1320

1380

gacatggaca tgacgctgaa caatccgggc tggagtcagc tgaccgcccc ctggttgaaa 1440

tcggcctga 1449

<210> 72

<211> 1569

<212> DNA

<213> view of Rahnella

<220>

<223> nifK strain CI019

<400> 72

atgagtcaag atcttggcac cccaaaatcc tgtttcccgc tgttcgagca ggatgaatac 60

cagagtatgt ttacccacaa acgcgcgctg gaagaagcac acggcgaggc gaaagtgcgg 120

gaagtgtttg aatggaccac cacgcaggaa tatcaggatc tgaacttctc gcgtgaagcg 180

ctgaccgtcg acccggcgaa agcctgccag ccgttaggcg cggtactttg cgcgctgggt 240

tttgccaaca cgttgccgta tgtccacggt tcacaaggct gtgtggcgta tttccgtacc 300

360

gccgccgttt ttggcggaaa taacaacatg aatgtcggtc tggaaaacgc cagcgcgctg 420

tacaagccgg aaattattgc tgtctccacc acctgtatgg cggaagtgat cggtgatgac 480

ctgcaggctt ttatcgccaa cgccaaaaaa gacggatttg tggatgccgg tatgccaatc 540

ccgtatgccc atacaccgag ttttctgggc agtcatgtca ccggctggga caacatgttt 600

gaaggcttcg cccgtacctt taccaccgac gccacgcggg aatatcagcc gggcaaactt 660

gccaaactga acgtggtgac cggttttgaa acttatctcg gcaactaccg ggttattcac 720

cgcatgatga gccagatggg ggtcgaatgc agcgtcttgt ccgatccgtc tgaagtgctc 780

gacaccccgg ctgacggcca ataccgcatg tatgccggcg gcaccacgca aaccgaaatg 840

cgtgatgcac cggatgccat cgacaccttg ctgctgcaac cgtggcaatt gcagaaaacc 900

aaaaaagtgg tgcagggcga ctggaatcag ccgggcaccg aagtcagtgt accgattggc 960

ctggcggcga ccgatgcctt gctgatgacg gtaagcgaac tgaccggcaa accgatagct 1020

gacacgctgg cgactgaacg tggccgtctg gtggacatga tgctcgattc ccacacctgg 1080

ctgcatggca agcgtttcgg tctctacggt gacccggatt ttgtgatgggg catgaccgca 1140

ttcctgctgg aactgggctg tgaaccgacc accattctca gccataacgg caacaaacgc 1200

tggcagaaag ccatgaagaa aatgctggct gattcgcctt acgggcagga cagcgaagtg 1260

tatgtgaact gcgatctgtg gcatttccgc tcgctgatgt ttacccgtaa accggacttt 1320

atgatcggca actcttacgg aaaattcatt cagcgtgaca cgctggccaa aggcgaacag 1380

ttcgaagtgc cgctgatccg catcggtttt ccgatttttg accggcacca tttgcaccgt 1440

cagaccacct ggggatacga aggggcgatg agcatactga cgcaactggt gaatgcggta 1500

cttgaacaac tggatcgcga aaccatgaag ctcggcaaaa ccgactacaa cttcgacctg 1560

atccgctaa 1569

<210> 73

<211> 1488

<212> DNA

<213> view of Rahnella

<220>

<223> nifL strain CI019

<400> 73

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gatttcgctg 120

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 180

240

catatctatg acgaaatgtg gcgcactttg ttgcaggggca aatcctggaa cggccaactg 300

atcaaccggc gtaataaccg ttcgctttat ctggcggatg tcactatcac gcctgtttta 360

ggcgcggacg ggcaggtgga gcattacctc ggcatgcaca aagatatcag cgagaaatac 420

gcgctggaac agcggttgcg caaccacatc accttgttca cggaggtgct gaacaatatt 480

cccgccgccg tggtggtggt ggatgagcag gacaatgtgg tgatggacaa tctggcctac 540

aaaacccttt gcgcggactg cggcggcaaa gagctgctgg ctgaaatgggg ctatccgcaa 600

ctcaaagaga tgctcaacag tggcgaaccg gtgccggttt ccatgcgcgg caacgtacgc 660

tggttttctt tcggtcaatg gttattgcag ggcgttaatg aagaggccag ccgctttttt 720

accggcatta ccgcgccggg aaaactgatt gttctgaccg actgcaccga tcagcatcac 780

cggcagcagc agggttatct tgaccggctt aagcaaaaac tcaccaacgg caaattattg 840

gcggccatcc gtgagtcgct cgatgccgcg cttatccagc tcaacgggcc aatcaatatg 900

ctggcggctg cgcgtcgtct taacggcgaa gaaggcaaca acatggcgct ggaattcgcc 960

tggcgcgaag gcgagcaggc ggtgagtcgc ttacaggcct gccgtccgtc gctggatttt 1020

gagccgcagg cagaatggcc ggtcagtgaa ttctttgacg atctgagcgc gctgtacgac 1080

agccattttc tcagtgacgg tgaattgcgt tacgtggtca tgccatctga tctgcacgct 1140

gtcgggcaac gaacgcaaat ccttaccgcg ctgagcttat ggattgatca cacgctgtca 1200

caggcgcagg ccatgccgtc tctgaagctc tcggtgaaca ttgttgcgag gcaggatgcg 1260

agctggttgt gttttgacat taccgataat gtgccgcgtg aacgggtgcg ttatgcccgc 1320

ccggaagcgg cgttttcccg tccggggaat ggcatggagc tgcgccttat cggacgctg 1380

atcgcccatc atcgcggttc tttagatctc tcggtccgcc ctgatggcgg caccttgctg 1440

acgttacgcc tgccggtaca gcaggttatc accggaggct taaaatga 1488

<210> 74

<211> 1557

<212> DNA

<213> view of Rahnella

<220>

<223> nifA strain CI019

<400> 74

atgacccagt tacctaccgc gggcccggtt atccggcgct ttgatatgtc tgcccagttt 60

acggcgcttt atcgcatcag cgtggcgctg agtcaggaaa gcaacaccgg gcgcgcactg 120

gcggcgatcc tcgaagtgct tcacgatcat gcatttatgc aatacggcat ggtgtgtctg 180

tttgataaag aacgcaatgc actctttgtg gaatccctgc atggcatcga cggcgaaagg 240

aaaaaagaga cccgccatgt ccgttaccgc atgggggaag gcgtgatcgg cgcggtgatg 300

agccagcgtc aggcgctggt gttaccgcgc atttcagacg atcagcgttt tctcgaccgc 360

ctgaatattt acgattacag cctgccgttg attggcgtgc cgatccccgg tgcggataat 420

cagccatcgg gcgtgctggt ggcacagccg atggcgttgc acgaagaccg gctgactgcc 480

agtacgcggt ttttagaaat ggtcgccaat ctcatcagcc agccactgcg ttctgccacg 540

ccccgggaat cattgcctgc tcaaacgccg gtccggtgca gtgttccgcg ccagtttggt 600

ttcgagcaga tggtcgggaa aagtcaggcg atgcgccaga cgatggacat tttacggcag 660

gtttccaaat gggataccac ggttctggtg cgtggtgaaa gcggcaccgg caaggaactt 720

atcgccaatg ccattcatta caactcaccc cgtgcggccg cgccatttgt gaaattcaac 780

tgcgccgcgc tgccggataa cctgctggaa agcgaactgt tcggtcatga aaaaggggcc 840

ttcaccggcg ctatccgtac ccgtaaaggc cgctttgaac tggcggacgg gggcacgtta 900

ttcctcgatg aaatcggcga atcgagcgcg tcgtttcagg ccaaattgct gcgcattttg 960

caggaaggtg aaatggaacg ggtcggcggc gataccacgc tgaaagttga tgtgcgcatt 1020

1080 attgctgcca ccaaccgtaa tcttgaagag gaagtgcgtg

ctgtattatc gcctgaacgt gatgccggtt tcgctgcctg cactgcgtga aaggctggat 1140

gatatcgccg atctggcgcc gtttctggtc aaaaagattg cgctgcgtca ggggcgggaa 1200

ctgcgcatca gcgacggtgc ggtgcgtctg ctgatgacct acagctggcc aggcaacgtg 1260

cgtgaactgg aaaactgtct cgaacgggcg tcggtaatga ccgatgaagg gctgatcgac 1320

cgcgacgtga tcctgttcaa tcaccatgaa tccccggcgc tgtccgtcaa acccggcctg 1380

ccgctcgcga cagatgaaag ctggctggat caggaactcg acgaacgcca gcgggtgatt 1440

gccgcactgg agaaaaccgg ctgggtgcag gccaaagcgg cccgactgct gggcatgaca 1500

ccgcgccaga ttgcctaccg tatccagatt atggacatca acatgcaccg tatctga 1557

<210> 75

<211> 1314

<212> DNA

<213> Kosakonia sacchari

<220>

<223> Prm5 with 500 bp flanking regions, strain CI006

<400> 75

aaaactaccg ccgcaattaa tgaacccaac gctactgttg ccgggccatg ctcttccccg 60

gcgcgctgcc cggaaaggat atagattgcc cagcacgcgc cagcaccaag cgcgaacgcc 120

gcgccagtga gatcaacatg tgaaacattt tcgcccagcg gcagcagata caagaggcca 180

agtaccgcca ggatcaccca gatgaaatcc accgggcggc gtgaggcaaa aagcgccacc 240

gccagcgggc cggtaaattc cagcgccacc gcaacgccga gcggtatcgt ctggatcgat 300

aaatagaaca tatagttcat ggcgccgagc gacaggccat aaaacagcag tggcaggcgt 360

tgttcacggg taaaatgtaa acgccagggc ttgaacacta cgaccaaaat aagggtgcca 420

agtgcgagac gcagcgcggt gacgccgggt gcgccaacaa tcggaaacag tgatttcgcc 480

agcgacgcgc ctccctgaat ggacatcatc gcgacaaaca atattaatac cggcaaccac 540

accggcaatt tacgagactg cgcaggcatc ctttctcccg tcaatttctg tcaaataaag 600

taaaagaggc agtctacttg aattaccccc ggctggttga gcgtttgttg aaaaaaagta 660

actgaaaaat ccgtagaata gcgccactct gatggttaat taacctattc aattaagaat 720

tatctggatg aatgtgccat taaatgcgca gcataatggt gcgttgtgcg ggaaaactgc 780

ttttttttga aagggttggt cagtagcgga aactttctgt tacatcaaat ggcgctttag 840

accccaattc ccgcaaagag tttcttaact aattttgata tatttaaacg cgtaggacgt 900

aggatttact tgaagcacat ttgaggtgga ttatgaaaaa aattgcatgt ctttcagcac 960

tggccgcact tctggcggtt tctgcaggtt ccgcagtagc agcaacttca accgtaactg 1020

gcggctacgc tcagagcgac gctcagggta ttgctaacaa aactaacggt ttcaacctga 1080

aatatcgcta cgagcaggac aacaacccgc tgggtgttat cggttccttt acttacactg 1140

aaaaagatcg caccgaaagc agcgtttata acaaagcgca gtactacggc atcaccgcag 1200

1260

gtaaattcca gcagactgta gacaccgcta aagtgtctga caccagcgac tacg 1314

<210> 76

<211> 3413

<212> DNA

<213> Kosakonia sacchari

<220>

<223> nifLA operon - upstream of the intergenic region and CDS nifL and nifA, strain CI006

<400> 76

aacacacgct cctgttgaaa aagagatccc gccgggaaat gcggtgaacg tgtctgatat 60

tgcgaagagt gtgccagttt tggtcgcggg caaaacctgc accagtttgg ttattaatgc 120

accagtctgg cgcttttttt cgccgagttt ctcctcgcta atgcccgcca ggcgcggctt 180

tggcgctgat agcgcgctga ataccgatct ggatcaaggt tttgtcgggt tatcagccaa 240

aaggtgcact ctttgcatgg ttatacgtgc ctgacatgtt gtccggggcga caaacggcct 300

ggtggcacaa attgtcagaa ctacgacacg actaactgac cgcaggagtg tgcgatgacc 360

ctgaatatga tgatggatgc cggcgcgcc gaggcaatcg ccggtgcgct ttcgcgacac 420

catcctgggc tgttttttac catcgttgaa gaagcgcccg tcgccatttc gctgactgat 480

gccgacgcac gcattgtcta tgccaacccg gctttctgcc gccagaccgg ctatgaacta 540

gaagcgttgt tgcagcaaaa tccccgcctg cttgcaagtc gccaaacccc acgggaaatc 600

tatcaggata tgtggcacac cttgttacaa cgccgaccgt ggcgcgggca attgattaac 660

cgccaccgcg acggcagcct gtatctggtc gagatcgata tcaccccggt gattaacccg 720

tttggcgaac tggaacacta cctggcaatg cagcgcgata tcagcgccag ttatgcgctg 780

gagcagcggt tgcgcaatca catgacgctg accgaagcgg tgctgaataa cattccggcg 840

gcggtggttg tagtggatga acgcgatcat gtggttatgg ataaccttgc ctacaaaacg 900

ttctgtgccg actgcggcgg aaaagagctc ctgagcgaac tcaatttttc agcccgaaaa 960

gcggagctgg caaacggcca ggtcttaccg gtggtgctgc gcggtgaggt gcgctggttg 1020

1080

1140

gaacagggcc gacttgaccg ccttaaacag cagatgacca acggcaaact actggcagcg 1200

atccgcgaag cgcttgacgc cgcgctgatc cagcttaact gccccatcaa tatgctggcg 1260

gcggcgcgac gtttaaacgg cagtgataac aacaatgtgg cgctcgacgc cgcgtggcgc 1320

gaaggtgaag aggcgatggc gcggctgaaa cgttgccgcc cgtcgctgga actggaaagt 1380

gcggccgtct ggccgctgca accctttttt gacgatctgc gcgcgcttta tcacacccgc 1440

tacgagcagg ggaaaaattt gcaggtcacg ctggattccc atcatctggt gggatttggt 1500

cagcgtacgc aactgttagc ctgcctgagt ctgtggctcg atcgcacgct ggatattgcc 1560

1620

ctctctttgt atatcactga caatgtgccg ctgatcccgc tgcgccacac ccactcgccg 1680

gatgcgctta acgctccggg aaaaggcatg gagctgcgcc tgatccagac gctggtggca 1740

caccaccacg gcgcaataga actcacttca caccccgaag ggggaagttg cctgacccta 1800

cgattcccgc tattcattc actgaccgga ggttcaaaat gacccagcga accgagtcgg 1860

gtaataccgt ctggcgcttc gatttgtccc agcagttcac tgcgatgcag cgcataagcg 1920

tggtactcag ccgggcgacc gaggtcgatc agacgctcca gcaagtgctg tgcgtattgc 1980

acaatgacgc ctttttgcag cacggcatga tctgtctgta cgacagccag caggcgattt 2040

tgaatattga agcgttgcag gaagccgatc agcagttaat ccccggcagc tcgcaaatcc 2100

gctatcgtcc gggcgaaggg ctggtcggga cggtgctttc gcagggccaa tcattagtgc 2160

tggcgcgcgt tgctgacgat cagcgctttc ttgaccggct cgggttgtat gattacaacc 2220

tgccgtttat cgccgtgccg ctgatagggc cagatgcgca gactttcggt gtgctgacgg 2280

cacaacccat ggcgcgttac gaagagcgat tacccgcctg cacccgcttt ctggaaacgg 2340

tcgctaacct ggtcgcgcaa accgtgcgtt tgatggcacc accggcagtg cgcccttccc 2400

cgcgcgccgc cataacacag gccgccagcc cgaaatcctg cacggcctca cgcgcatttg 2460

gttttgaaaa tatggtcggt aacagtccgg cgatgcgcca gaccatggag attatccgtc 2520

aggtttcgcg ctgggacacc accgttctgg tacgcggcga gagtggcacc ggcaaggagc 2580

tgattgccaa cgccatccac caccattcgc cgcgtgccgg tgcgccattt gtgaaattca 2640

actgtgcggc gctgccggac acactgctgg aaagcgaatt gttcggtcac gagaaagggg 2700

catttaccgg cgcggtacgc cagcgtaaag gccgttttga gctggccgat ggcggcacgc 2760

tgtttcttga cgagatcggc gagagtagcg cctcgtttca ggctaagctg ctgcgcattt 2820

tgcaggaagg cgaaatggaa cgcgtcggcg gcgacgagac attgcaagtg aatgtgcgca 2880

ttattgccgc gacgaaccgc aatcttgaag atgaagtccg gctggggcac tttcgcgaag 2940

atctctatta tcgcctgaat gtgatgccca tcgccctgcc gccactacgc gaacgccagg 3000

aggacattgc cgagctggcg cactttctgg tgcgtaaaat cgcccataac cagagccgta 3060

cgctgcgcat tagcgagggc gctatccgcc tgctgatgag ctacaactgg cccggtaatg 3120

tgcgcgaact ggaaaactgc cttgagcgct cagcggtgat gtcggagaac ggtctgatcg 3180

atcgggatgt gattttgttt aatcatcgcg accagccagc caaaccgcca gttatcagcg 3240

tctcgcatga tgataactgg ctcgataaca accttgacga gcgccagcgg ctgattgcgg 3300

cgctggaaaa agcgggatgg gtacaagcca aagccgcgcg cttgctgggg atgacgccgc 3360

gccaggtcgc ctatcgtatt cagacgatgg atataaccct gccaaggcta taa 3413

<210> 77

<211> 495

<212> Protein

<213> Kosakonia sacchari

<220>

<223> NifL strain CI006

<400> 77

Met Thr Leu Asn Met Met Met Asp Ala Gly Ala Pro Glu Ala Ile Ala

1 5 10 15

Gly Ala Leu Ser Arg His His Pro Gly Leu Phe Phe Thr Ile Val Glu

20 25 30

Glu Ala Pro Val Ala Ile Ser Leu Thr Asp Ala Asp Ala Arg Ile Val

35 40 45

Tyr Ala Asn Pro Ala Phe Cys Arg Gln Thr Gly Tyr Glu Leu Glu Ala

50 55 60

Leu Leu Gln Gln Asn Pro Arg Leu Leu Ala Ser Arg Gln Thr Pro Arg

65 70 75 80

Glu Ile Tyr Gln Asp Met Trp His Thr Leu Leu Gln Arg Arg Pro Trp

85 90 95

Arg Gly Gln Leu Ile Asn Arg His Arg Asp Gly Ser Leu Tyr Leu Val

100 105 110

Glu Ile Asp Ile Thr Pro Val Ile Asn Pro Phe Gly Glu Leu Glu His

115 120 125

Tyr Leu Ala Met Gln Arg Asp Ile Ser Ala Ser Tyr Ala Leu Glu Gln

130 135 140

Arg Leu Arg Asn His Met Thr Leu Thr Glu Ala Val Leu Asn Asn Ile

145 150 155 160

Pro Ala Ala Val Val Val Val Asp Glu Arg Asp His Val Val Met Asp

165 170 175

Asn Leu Ala Tyr Lys Thr Phe Cys Ala Asp Cys Gly Gly Lys Glu Leu

180 185 190

Leu Ser Glu Leu Asn Phe Ser Ala Arg Lys Ala Glu Leu Ala Asn Gly

195 200 205

Gln Val Leu Pro Val Val Leu Arg Gly Glu Val Arg Trp Leu Ser Val

210 215 220

Thr Cys Trp Ala Leu Pro Gly Val Ser Glu Glu Ala Ser Arg Tyr Phe

225 230 235 240

Ile Asp Asn Arg Leu Thr Arg Thr Leu Val Val Ile Thr Asp Asp Thr

245 250 255

Gln Gln Arg Gln Gln Gln Glu Gln Gly Arg Leu Asp Arg Leu Lys Gln

260 265 270

Gln Met Thr Asn Gly Lys Leu Leu Ala Ala Ile Arg Glu Ala Leu Asp

275 280 285

Ala Ala Leu Ile Gln Leu Asn Cys Pro Ile Asn Met Leu Ala Ala Ala

290 295 300

Arg Arg Leu Asn Gly Ser Asp Asn Asn Asn Val Ala Leu Asp Ala Ala

305 310 315 320

Trp Arg Glu Gly Glu Glu Glu Ala Met Ala Arg Leu Lys Arg Cys Arg Pro

325 330 335

Ser Leu Glu Leu Glu Ser Ala Ala Val Trp Pro Leu Gln Pro Phe Phe

340 345 350

Asp Asp Leu Arg Ala Leu Tyr His Thr Arg Tyr Glu Gln Gly Lys Asn

355 360 365

Leu Gln Val Thr Leu Asp Ser His His Leu Val Gly Phe Gly Gln Arg

370 375 380

Thr Gln Leu Leu Ala Cys Leu Ser Leu Trp Leu Asp Arg Thr Leu Asp

385 390 395 400

Ile Ala Ala Gly Leu Gly Asp Phe Thr Ala Gln Thr Gln Ile Tyr Ala

405 410 415

Arg Glu Glu Glu Gly Trp Leu Ser Leu Tyr Ile Thr Asp Asn Val Pro

420 425 430

Leu Ile Pro Leu Arg His Thr His Ser Pro Asp Ala Leu Asn Ala Pro

435 440 445

Gly Lys Gly Met Glu Leu Arg Leu Ile Gln Thr Leu Val Ala His His

450 455 460

His Gly Ala Ile Glu Leu Thr Ser His Pro Glu Gly Gly Ser Cys Leu

465 470 475 480

Thr Leu Arg Phe Pro Leu Phe His Ser Leu Thr Gly Gly Ser Lys

485 490 495

<210> 78

<211> 524

<212> Protein

<213> Kosakonia sacchari

<220>

<223> NifA strain CI006

<400> 78

Met Thr Gln Arg Thr Glu Ser Gly Asn Thr Val Trp Arg Phe Asp Leu

1 5 10 15

Ser Gln Gln Phe Thr Ala Met Gln Arg Ile Ser Val Val Leu Ser Arg

20 25 30

Ala Thr Glu Val Asp Gln Thr Leu Gln Gln Val Leu Cys Val Leu His

35 40 45

Asn Asp Ala Phe Leu Gln His Gly Met Ile Cys Leu Tyr Asp Ser Gln

50 55 60

Gln Ala Ile Leu Asn Ile Glu Ala Leu Gln Glu Ala Asp Gln Gln Leu

65 70 75 80

Ile Pro Gly Ser Ser Gln Ile Arg Tyr Arg Pro Gly Glu Gly Leu Val

85 90 95

Gly Thr Val Leu Ser Gln Gly Gln Ser Leu Val Leu Ala Arg Val Ala

100 105 110

Asp Asp Gln Arg Phe Leu Asp Arg Leu Gly Leu Tyr Asp Tyr Asn Leu

115 120 125

Pro Phe Ile Ala Val Pro Leu Ile Gly Pro Asp Ala Gln Thr Phe Gly

130 135 140

Val Leu Thr Ala Gln Pro Met Ala Arg Tyr Glu Glu Glu Arg Leu Pro Ala

145 150 155 160

Cys Thr Arg Phe Leu Glu Thr Val Ala Asn Leu Val Ala Gln Thr Val

165 170 175

Arg Leu Met Ala Pro Pro Ala Val Arg Pro Ser Pro Arg Ala Ala Ile

180 185 190

Thr Gln Ala Ala Ser Pro Lys Ser Cys Thr Ala Ser Arg Ala Phe Gly

195 200 205

Phe Glu Asn Met Val Gly Asn Ser Pro Ala Met Arg Gln Thr Met Glu

210 215 220

Ile Ile Arg Gln Val Ser Arg Trp Asp Thr Thr Val Leu Val Arg Gly

225 230 235 240

Glu Ser Gly Thr Gly Lys Glu Leu Ile Ala Asn Ala Ile His His His

245 250 255

Ser Pro Arg Ala Gly Ala Pro Phe Val Lys Phe Asn Cys Ala Ala Leu

260 265 270

Pro Asp Thr Leu Leu Glu Ser Glu Leu Phe Gly His Glu Lys Gly Ala

275 280 285

Phe Thr Gly Ala Val Arg Gln Arg Lys Gly Arg Phe Glu Leu Ala Asp

290 295 300

Gly Gly Thr Leu Phe Leu Asp Glu Ile Gly Glu Ser Ser Ala Ser Phe

305 310 315 320

Gln Ala Lys Leu Leu Arg Ile Leu Gln Glu Gly Glu Met Glu Arg Val

325 330 335

Gly Gly Asp Glu Thr Leu Gln Val Asn Val Arg Ile Ile Ala Ala Thr

340 345 350

Asn Arg Asn Leu Glu Asp Glu Val Arg Leu Gly His Phe Arg Glu Asp

355 360 365

Leu Tyr Tyr Arg Leu Asn Val Met Pro Ile Ala Leu Pro Pro Leu Arg

370 375 380

Glu Arg Gln Glu Asp Ile Ala Glu Leu Ala His Phe Leu Val Arg Lys

385 390 395 400

Ile Ala His Asn Gln Ser Arg Thr Leu Arg Ile Ser Glu Gly Ala Ile

405 410 415

Arg Leu Leu Met Ser Tyr Asn Trp Pro Gly Asn Val Arg Glu Leu Glu

420 425 430

Asn Cys Leu Glu Arg Ser Ala Val Met Ser Glu Asn Gly Leu Ile Asp

435 440 445

Arg Asp Val Ile Leu Phe Asn His Arg Asp Gln Pro Ala Lys Pro Pro

450 455 460

Val Ile Ser Val Ser His Asp Asp Asn Trp Leu Asp Asn Asn Leu Asp

465 470 475 480

Glu Arg Gln Arg Leu Ile Ala Ala Leu Glu Lys Ala Gly Trp Val Gln

485 490 495

Ala Lys Ala Ala Arg Leu Leu Gly Met Thr Pro Arg Gln Val Ala Tyr

500 505 510

Arg Ile Gln Thr Met Asp Ile Thr Leu Pro Arg Leu

515 520

<210> 79

<211> 2850

<212> DNA

<213> Kosakonia sacchari

<220>

<223> glnE strain CI006

<400> 79

atgccgcacc acgcaggatt gtcgcagcac tggcaaacgg tattttctcg tctgccggaa 60

tcgctcaccg cgcagccatt gagcgcgcag gcgcagtcag tgctcacttt tagtgatttt 120

gttcaggaca gcatcatcgc gcatcctgag tggctggcag agcttgaaag cgcgccgccg 180

cctgcgaacg aatggcaaca ctatgcgcaa tggctgcaag cggcgctgga tggcgtcacc 240

gatgaagcct cgctgatgcg cgcgctgcgg ctgtttcgcc gtcgcatcat ggtgcgcatc 300

gcctggagcc aggcgttaca gttggtggcg gaagaagata tcctgcaaca gcttagcgtg 360

ctggcggaaa ccctgatcgt cgccgcgcgc gactggcttt atgaggcctg ctgccgtgag 420

tggggaacgc cgagcaatcc acaaggcgtg gcgcagccga tgctggtact cggcatggggc 480

aaactgggtg gcggcgaact caatttctca tccgatatcg atttgatttt cgcctggccg 540

gaaaatggcg caacgcgcgg tggacgccgt gagctggata acgcgcaatt tttcactcgc 600

cttggtcaac ggctgattaa agtcctcgac cagccaacgc aggatggctt tgtctaccgc 660

gtcgatatgc gcttgcgccc gtttggcgac agcggcccgc tggtgctgag ctttgccgcg 720

ctggaagatt actaccagga gcaggggcgc gattgggaac gctacgcgat ggtgaaagcg 780

cgcattatgg gcgataacga cggcgaccat gcgcgggagt tgcgcgcaat gctgcgcccg 840

tttgttttcc gccgttatat cgacttcagc gtgattcagt ccctgcgtaa catgaaaggc 900

atgattgccc gcgaagtgcg tcgccgtggc ctgaaggaca acattaagct cggcgcgggc 960

gggatccgcg aaatagaatt tatcgtccag gttttccagc tgattcgcgg cggtcgcgag 1020

cctgcactgc aatcgcgttc actgttgccg acgcttgctg ccatagatca actgcatctg 1080

ctgccggatg gcgacgcaac ccggctgcgc gaggcgtatt tgtggctgcg acggctggag 1140

aacctgctgc aaagcatcaa tgacgaacag aacacagacgc tgccgggcga tgaactgaat 1200

cgcgcgcgcc tcgcctgggg aatgggcaaa gatagctggg aagcgctctg cgaaacgctg 1260

1320

1380

gaggaggatt ccacgcccgt gctggcgcat ctctcagagg acgatcgccg ccgcgtggtg 1440

gcgctgattg ccgattttcg caaagagttg gataaacgca ccattggccc gcgagggcgg 1500

caggtactcg atcacttaat gccgcatctg ctcagcgatg tatgctcgcg cgacgatgcg 1560

ccagtaccgc tgtcacgcct gacgccgctg ctcaccggaa ttattacccg caccacttac 1620

cttgagctgc taagtgaatt tcccggcgca ctgaaacacc tcatttccct gtgtgccgcg 1680

tcgccgatgg ttgccagtca gctggcgcgc tacccgatcc tgcttgatga attgctcgac 1740

ccgaatacgc tctatcaacc gacggcgatg aatgcctatc gcgatgagct gcgccaatac 1800

ctgctgcgcg tgccggaaga tgatgaagag caacagcttg aggcgctgcg gcagtttaag 1860

caggcgcagt tgctgcgcgt ggcggcggcg gatattgccg gtacgttgcc agtaatgaaa 1920

gtgagcgatc acttaacctg gctggcggaa gcgattattg atgcggtggt gcagcaagcc 1980

tgggggcaga tggtggcgcg ttatggccag ccaacgcatc tgcacgatcg cgaagggcgc 2040

ggttttgcgg tggtcggtta tggcaagctg ggcggctggg agctgggtta cagctccgat 2100

ctggatctgg tattcctgca cgactgcccg atggatgtga tgaccgatgg cgagcgtgaa 2160

atcgatggtc gccagttcta tttgcgtctc gcgcagcgcg tgatgcacct gtttagcacg 2220

cgcacgtcgt ccggcatcct ttatgaagtt gatgcgcgtc tgcgtccatc tggcgctgcg 2280

gggatgctgg tcactactac ggaatcgttc gccgattacc agcaaaacga agcctggacg 2340

tgggaacatc aggcgctggc ccgtgcgcgc gtggtgtacg gcgatccgca actgaccgcc 2400

gaatttgacg ccattcgccg cgatattctg atgacgcctc gcgacggcgc aacgctgcaa 2460

accgacgtgc gagaaatgcg cgagaaaatg cgtgcccatc ttggcaacaa gcataaagac 2520

2580 cgcttcgatc tgaaagccga tgaaggcggt

ctggtgctgc gctttgccca tgacaagccg aaactgacgc gctggtcgga taatgtgcgc 2640

attctcgaag ggctggcgca aaacggcatc atggaggagc aggaagcgca ggcattgacg 2700

ctggcgtaca ccacattgcg tgatgagctg caccacctgg cgctgcaaga gttgccggga 2760

catgtggcgc tctcctgttt tgtcgccgag cgtgcgctta ttaaaaccag ctgggacaag 2820

tggctggtgg aaccgtgcgc cccggcgtaa 2850

<210> 80

<211> 1563

<212> DNA

<213> Kosakonia sacchari

<220>

<223> glnE_KO1, strain CI006

<400> 80

atgtttaacg atctgattgg cgatgatgaa acggattcgc cggaagatgc gctttctgag 60

agctggcgcg aattgtggca ggatgcgttg caggaggagg attccacgcc cgtgctggcg 120

catctctcag aggacgatcg ccgccgcgtg gtggcgctga ttgccgattt tcgcaaagag 180

ttggataaac gcaccattgg cccgcgaggg cggcaggtac tcgatcactt aatgccgcat 240

ctgctcagcg atgtatgctc gcgcgacgat gcgccagtac cgctgtcacg cctgacgccg 300

ctgctcaccg gaattattac ccgcaccact taccttgagc tgctaagtga atttcccggc 360

gcactgaaac acctcatttc cctgtgtgcc gcgtcgccga tggttgccag tcagctggcg 420

cgctacccga tcctgcttga tgaattgctc gacccgaata cgctctatca accgacggcg 480

atgaatgcct atcgcgatga gctgcgccaa tacctgctgc gcgtgccgga agatgatgaa 540

gagcaacagc ttgaggcgct gcggcagttt aagcaggcgc agttgctgcg cgtggcggcg 600

gcggatattg ccggtacgtt gccagtaatg aaagtgagcg atcacttaac ctggctggcg 660

gaagcgatta ttgatgcggt ggtgcagcaa gcctgggggc agatggtggc gcgttatggc 720

cagccaacgc atctgcacga tcgcgaaggg cgcggttttg cggtggtcgg ttatggcaag 780

ctgggcggct gggagctggg ttacagctcc gatctggatc tggtattcct gcacgactgc 840

ccgatggatg tgatgaccga tggcgagcgt gaaatcgatg gtcgccagtt ctatttgcgt 900

ctcgcgcagc gcgtgatgca cctgtttagc acgcgcacgt cgtccggcat cctttatgaa 960

gttgatgcgc gtctgcgtcc atctggcgct gcggggatgc tggtcactac tacggaatcg 1020

ttcgccgatt accagcaaaa cgaagcctgg acgtgggaac atcaggcgct ggcccgtgcg 1080

cgcgtggtgt acggcgatcc gcaactgacc gccgaatttg acgccattcg ccgcgatatt 1140

ctgatgacgc ctcgcgacgg cgcaacgctg caaaccgacg tgcgagaaat gcgcgagaaa 1200

atgcgtgccc atcttggcaa caagcataaa gaccgcttcg atctgaaagc cgatgaaggc 1260

ggtatcaccg acatcgagtt tatcgcccaa tatctggtgc tgcgctttgc ccatgacaag 1320

ccgaaactga cgcgctggtc ggataatgtg cgcattctcg aagggctggc gcaaaacggc 1380

atcatggagg agcaggaagc gcaggcattg acgctggcgt acaccacatt gcgtgatgag 1440

ctgcaccacc tggcgctgca agagttgccg ggacatgtgg cgctctcctg ttttgtcgcc 1500

1560 gagcgtgcgc ttattaaaac cagctgggac

taa 1563

<210> 81

<211> 949

<212> Protein

<213> Kosakonia sacchari

<220>

<223> glnE strain CI006

<400> 81

Met Pro His His Ala Gly Leu Ser Gln His Trp Gln Thr Val Phe Ser

1 5 10 15

Arg Leu Pro Glu Ser Leu Thr Ala Gln Pro Leu Ser Ala Gln Ala Gln

20 25 30

Ser Val Leu Thr Phe Ser Asp Phe Val Gln Asp Ser Ile Ile Ala His

35 40 45

Pro Glu Trp Leu Ala Glu Leu Glu Ser Ala Pro Pro Pro Ala Asn Glu

50 55 60

Trp Gln His Tyr Ala Gln Trp Leu Gln Ala Ala Leu Asp Gly Val Thr

65 70 75 80

Asp Glu Ala Ser Leu Met Arg Ala Leu Arg Leu Phe Arg Arg Arg Ile

85 90 95

Met Val Arg Ile Ala Trp Ser Gln Ala Leu Gln Leu Val Ala Glu Glu

100 105 110

Asp Ile Leu Gln Gln Leu Ser Val Leu Ala Glu Thr Leu Ile Val Ala

115 120 125

Ala Arg Asp Trp Leu Tyr Glu Ala Cys Cys Arg Glu Trp Gly Thr Pro

130 135 140

Ser Asn Pro Gln Gly Val Ala Gln Pro Met Leu Val Leu Gly Met Gly

145 150 155 160

Lys Leu Gly Gly Gly Glu Leu Asn Phe Ser Ser Asp Ile Asp Leu Ile

165 170 175

Phe Ala Trp Pro Glu Asn Gly Ala Thr Arg Gly Gly Arg Arg Glu Leu

180 185 190

Asp Asn Ala Gln Phe Phe Thr Arg Leu Gly Gln Arg Leu Ile Lys Val

195 200 205

Leu Asp Gln Pro Thr Gln Asp Gly Phe Val Tyr Arg Val Asp Met Arg

210 215 220

Leu Arg Pro Phe Gly Asp Ser Gly Pro Leu Val Leu Ser Phe Ala Ala

225 230 235 240

Leu Glu Asp Tyr Tyr Gln Glu Gln Gly Arg Asp Trp Glu Arg Tyr Ala

245 250 255

Met Val Lys Ala Arg Ile Met Gly Asp Asn Asp Gly Asp His Ala Arg

260 265 270

Glu Leu Arg Ala Met Leu Arg Pro Phe Val Phe Arg Arg Tyr Ile Asp

275 280 285

Phe Ser Val Ile Gln Ser Leu Arg Asn Met Lys Gly Met Ile Ala Arg

290 295 300

Glu Val Arg Arg Arg Gly Leu Lys Asp Asn Ile Lys Leu Gly Ala Gly

305 310 315 320

Gly Ile Arg Glu Ile Glu Phe Ile Val Gln Val Phe Gln Leu Ile Arg

325 330 335

Gly Gly Arg Glu Pro Ala Leu Gln Ser Arg Ser Leu Leu Pro Thr Leu

340 345 350

Ala Ala Ile Asp Gln Leu His Leu Leu Pro Asp Gly Asp Ala Thr Arg

355 360 365

Leu Arg Glu Ala Tyr Leu Trp Leu Arg Arg Leu Glu Asn Leu Leu Gln

370 375 380

Ser Ile Asn Asp Glu Gln Thr Gln Thr Leu Pro Gly Asp Glu Leu Asn

385 390 395 400

Arg Ala Arg Leu Ala Trp Gly Met Gly Lys Asp Ser Trp Glu Ala Leu

405 410 415

Cys Glu Thr Leu Glu Ala His Met Ser Ala Val Arg Gln Ile Phe Asn

420 425 430

Asp Leu Ile Gly Asp Asp Glu Thr Asp Ser Pro Glu Asp Ala Leu Ser

435 440 445

Glu Ser Trp Arg Glu Leu Trp Gln Asp Ala Leu Gln Glu Glu Asp Ser

450 455 460

Thr Pro Val Leu Ala His Leu Ser Glu Asp Asp Arg Arg Arg Val Val

465 470 475 480

Ala Leu Ile Ala Asp Phe Arg Lys Glu Leu Asp Lys Arg Thr Ile Gly

485 490 495

Pro Arg Gly Arg Gln Val Leu Asp His Leu Met Pro His Leu Leu Ser

500 505 510

Asp Val Cys Ser Arg Asp Asp Ala Pro Val Pro Leu Ser Arg Leu Thr

515 520 525

Pro Leu Leu Thr Gly Ile Ile Thr Arg Thr Thr Tyr Leu Glu Leu Leu

530 535 540

Ser Glu Phe Pro Gly Ala Leu Lys His Leu Ile Ser Leu Cys Ala Ala

545 550 555 560

Ser Pro Met Val Ala Ser Gln Leu Ala Arg Tyr Pro Ile Leu Leu Asp

565 570 575

Glu Leu Leu Asp Pro Asn Thr Leu Tyr Gln Pro Thr Ala Met Asn Ala

580 585 590

Tyr Arg Asp Glu Leu Arg Gln Tyr Leu Leu Arg Val Pro Glu Asp Asp

595 600 605

Glu Glu Gln Gln Leu Glu Ala Leu Arg Gln Phe Lys Gln Ala Gln Leu

610 615 620

Leu Arg Val Ala Ala Ala Asp Ile Ala Gly Thr Leu Pro Val Met Lys

625 630 635 640

Val Ser Asp His Leu Thr Trp Leu Ala Glu Ala Ile Ile Asp Ala Val

645 650 655

Val Gln Gln Ala Trp Gly Gln Met Val Ala Arg Tyr Gly Gln Pro Thr

660 665 670

His Leu His Asp Arg Glu Gly Arg Gly Phe Ala Val Val Gly Tyr Gly

675 680 685

Lys Leu Gly Gly Trp Glu Leu Gly Tyr Ser Ser Asp Leu Asp Leu Val

690 695 700

Phe Leu His Asp Cys Pro Met Asp Val Met Thr Asp Gly Glu Arg Glu

705 710 715 720

Ile Asp Gly Arg Gln Phe Tyr Leu Arg Leu Ala Gln Arg Val Met His

725 730 735

Leu Phe Ser Thr Arg Thr Ser Ser Gly Ile Leu Tyr Glu Val Asp Ala

740 745 750

Arg Leu Arg Pro Ser Gly Ala Ala Gly Met Leu Val Thr Thr Thr Glu

755 760 765

Ser Phe Ala Asp Tyr Gln Gln Asn Glu Ala Trp Thr Trp Glu His Gln

770 775 780

Ala Leu Ala Arg Ala Arg Val Val Tyr Gly Asp Pro Gln Leu Thr Ala

785 790 795 800

Glu Phe Asp Ala Ile Arg Arg Asp Ile Leu Met Thr Pro Arg Asp Gly

805 810 815

Ala Thr Leu Gln Thr Asp Val Arg Glu Met Arg Glu Lys Met Arg Ala

820 825 830

His Leu Gly Asn Lys His Lys Asp Arg Phe Asp Leu Lys Ala Asp Glu

835 840 845

Gly Gly Ile Thr Asp Ile Glu Phe Ile Ala Gln Tyr Leu Val Leu Arg

850 855 860

Phe Ala His Asp Lys Pro Lys Leu Thr Arg Trp Ser Asp Asn Val Arg

865 870 875 880

Ile Leu Glu Gly Leu Ala Gln Asn Gly Ile Met Glu Glu Gln Glu Ala

885 890 895

Gln Ala Leu Thr Leu Ala Tyr Thr Thr Leu Arg Asp Glu Leu His His

900 905 910

Leu Ala Leu Gln Glu Leu Pro Gly His Val Ala Leu Ser Cys Phe Val

915 920 925

Ala Glu Arg Ala Leu Ile Lys Thr Ser Trp Asp Lys Trp Leu Val Glu

930 935 940

Pro Cys Ala Pro Ala

945

<210> 82

<211> 520

<212> Protein

<213> Kosakonia sacchari

<220>

<223> GlnE_KO1 strain CI006

<400> 82

Met Phe Asn Asp Leu Ile Gly Asp Asp Glu Thr Asp Ser Pro Glu Asp

1 5 10 15

Ala Leu Ser Glu Ser Trp Arg Glu Leu Trp Gln Asp Ala Leu Gln Glu

20 25 30

Glu Asp Ser Thr Pro Val Leu Ala His Leu Ser Glu Asp Asp Arg Arg

35 40 45

Arg Val Val Ala Leu Ile Ala Asp Phe Arg Lys Glu Leu Asp Lys Arg

50 55 60

Thr Ile Gly Pro Arg Gly Arg Gln Val Leu Asp His Leu Met Pro His

65 70 75 80

Leu Leu Ser Asp Val Cys Ser Arg Asp Asp Ala Pro Val Pro Leu Ser

85 90 95

Arg Leu Thr Pro Leu Leu Thr Gly Ile Ile Thr Arg Thr Thr Tyr Leu

100 105 110

Glu Leu Leu Ser Glu Phe Pro Gly Ala Leu Lys His Leu Ile Ser Leu

115 120 125

Cys Ala Ala Ser Pro Met Val Ala Ser Gln Leu Ala Arg Tyr Pro Ile

130 135 140

Leu Leu Asp Glu Leu Leu Asp Pro Asn Thr Leu Tyr Gln Pro Thr Ala

145 150 155 160

Met Asn Ala Tyr Arg Asp Glu Leu Arg Gln Tyr Leu Leu Arg Val Pro

165 170 175

Glu Asp Asp Glu Glu Gln Gln Leu Glu Ala Leu Arg Gln Phe Lys Gln

180 185 190

Ala Gln Leu Leu Arg Val Ala Ala Ala Asp Ile Ala Gly Thr Leu Pro

195 200 205

Val Met Lys Val Ser Asp His Leu Thr Trp Leu Ala Glu Ala Ile Ile

210 215 220

Asp Ala Val Val Gln Gln Ala Trp Gly Gln Met Val Ala Arg Tyr Gly

225 230 235 240

Gln Pro Thr His Leu His Asp Arg Glu Gly Arg Gly Phe Ala Val Val

245 250 255

Gly Tyr Gly Lys Leu Gly Gly Trp Glu Leu Gly Tyr Ser Ser Asp Leu

260 265 270

Asp Leu Val Phe Leu His Asp Cys Pro Met Asp Val Met Thr Asp Gly

275 280 285

Glu Arg Glu Ile Asp Gly Arg Gln Phe Tyr Leu Arg Leu Ala Gln Arg

290 295 300

Val Met His Leu Phe Ser Thr Arg Thr Ser Ser Gly Ile Leu Tyr Glu

305 310 315 320

Val Asp Ala Arg Leu Arg Pro Ser Gly Ala Ala Gly Met Leu Val Thr

325 330 335

Thr Thr Glu Ser Phe Ala Asp Tyr Gln Gln Asn Glu Ala Trp Thr Trp

340 345 350

Glu His Gln Ala Leu Ala Arg Ala Arg Val Val Tyr Gly Asp Pro Gln

355 360 365

Leu Thr Ala Glu Phe Asp Ala Ile Arg Arg Asp Ile Leu Met Thr Pro

370 375 380

Arg Asp Gly Ala Thr Leu Gln Thr Asp Val Arg Glu Met Arg Glu Lys

385 390 395 400

Met Arg Ala His Leu Gly Asn Lys His Lys Asp Arg Phe Asp Leu Lys

405 410 415

Ala Asp Glu Gly Gly Ile Thr Asp Ile Glu Phe Ile Ala Gln Tyr Leu

420 425 430

Val Leu Arg Phe Ala His Asp Lys Pro Lys Leu Thr Arg Trp Ser Asp

435 440 445

Asn Val Arg Ile Leu Glu Gly Leu Ala Gln Asn Gly Ile Met Glu Glu

450 455 460

Gln Glu Ala Gln Ala Leu Thr Leu Ala Tyr Thr Thr Leu Arg Asp Glu

465 470 475 480

Leu His His Leu Ala Leu Gln Glu Leu Pro Gly His Val Ala Leu Ser

485 490 495

Cys Phe Val Ala Glu Arg Ala Leu Ile Lys Thr Ser Trp Asp Lys Trp

500 505 510

Leu Val Glu Pro Cys Ala Pro Ala

515 520

<210> 83

<211> 341

<212> Protein

<213> Kosakonia sacchari

<220>

<223> 3ATase domain, strain CI006

<400> 83

Glu Glu Gln Gln Leu Glu Ala Leu Arg Gln Phe Lys Gln Ala Gln Leu

1 5 10 15

Leu Arg Val Ala Ala Ala Asp Ile Ala Gly Thr Leu Pro Val Met Lys

20 25 30

Val Ser Asp His Leu Thr Trp Leu Ala Glu Ala Ile Ile Asp Ala Val

35 40 45

Val Gln Gln Ala Trp Gly Gln Met Val Ala Arg Tyr Gly Gln Pro Thr

50 55 60

His Leu His Asp Arg Glu Gly Arg Gly Phe Ala Val Val Gly Tyr Gly

65 70 75 80

Lys Leu Gly Gly Trp Glu Leu Gly Tyr Ser Ser Asp Leu Asp Leu Val

85 90 95

Phe Leu His Asp Cys Pro Met Asp Val Met Thr Asp Gly Glu Arg Glu

100 105 110

Ile Asp Gly Arg Gln Phe Tyr Leu Arg Leu Ala Gln Arg Val Met His

115 120 125

Leu Phe Ser Thr Arg Thr Ser Ser Gly Ile Leu Tyr Glu Val Asp Ala

130 135 140

Arg Leu Arg Pro Ser Gly Ala Ala Gly Met Leu Val Thr Thr Thr Glu

145 150 155 160

Ser Phe Ala Asp Tyr Gln Gln Asn Glu Ala Trp Thr Trp Glu His Gln

165 170 175

Ala Leu Ala Arg Ala Arg Val Val Tyr Gly Asp Pro Gln Leu Thr Ala

180 185 190

Glu Phe Asp Ala Ile Arg Arg Asp Ile Leu Met Thr Pro Arg Asp Gly

195 200 205

Ala Thr Leu Gln Thr Asp Val Arg Glu Met Arg Glu Lys Met Arg Ala

210 215 220

His Leu Gly Asn Lys His Lys Asp Arg Phe Asp Leu Lys Ala Asp Glu

225 230 235 240

Gly Gly Ile Thr Asp Ile Glu Phe Ile Ala Gln Tyr Leu Val Leu Arg

245 250 255

Phe Ala His Asp Lys Pro Lys Leu Thr Arg Trp Ser Asp Asn Val Arg

260 265 270

Ile Leu Glu Gly Leu Ala Gln Asn Gly Ile Met Glu Glu Gln Glu Ala

275 280 285

Gln Ala Leu Thr Leu Ala Tyr Thr Thr Leu Arg Asp Glu Leu His His

290 295 300

Leu Ala Leu Gln Glu Leu Pro Gly His Val Ala Leu Ser Cys Phe Val

305 310 315 320

Ala Glu Arg Ala Leu Ile Lys Thr Ser Trp Asp Lys Trp Leu Val Glu

325 330 335

Pro Cys Ala Pro Ala

340

<210> 84

<211> 1342

<212> DNA

<213> Kosakonia sacchari

<220>

<223> Prm5 embedded in the nifL region, strain CI006, currently strain CM029

<400> 84

cggagcgtcg gggtgcctaa tatcagcacc ggatacgaga gaaaagtgtc tacatcggtt 60

cggttgatat tgaccggcgc atccgccagc ccgcccagtt tctggtggat ctgtttggcg 120

attttgcggg tcttgccggt gtcggtgccg aaaaaaatac caatatttgc cataacacac 180

gctcctgttg aaaaagagat cccgccggga aatgcggtga acgtgtctga tattgcgaag 240

agtgtgccag ttttggtcgc gggcaaaacc tgcaccagtt tggttattaa tgcaccagtc 300

360

gatagcgcgc tgaataccga tctggatcaa ggttttgtcg ggttatcagc caaaaggtgc 420

actctttgca tggttatacg tgcctgacat gttgtccggg cgacaaacgg cctggtggca 480

caaattgtca gaactacgac acgactaact gaccgcagga gtgtgcgatg accctgaata 540

tgatgatgga tgccggcgga catcatcgcg acaaacaata ttaataccgg caaccacacc 600

ggcaatttac gagactgcgc aggcatcctt tctcccgtca atttctgtca aataaagtaa 660

aagaggcagt ctacttgaat tacccccggc tggttgagcg tttgttgaaa aaaagtaact 720

gaaaaatccg tagaatagcg ccactctgat ggttaattaa cctattcaat taagaattat 780

ctggatgaat gtgccattaa atgcgcagca taatggtgcg ttgtgcggga aaactgcttt 840

tttttgaaag ggttggtcag tagcggaaac aactcacttc acaccccgaa gggggaagtt 900

gcctgaccct acgattcccg ctatttcatt cactgaccgg aggttcaaaa tgacccagcg 960

aaccgagtcg ggtaataccg tctggcgctt cgatttgtcc cagcagttca ctgcgatgca 1020

gcgcataagc gtggtactca gccgggcgac cgaggtcgat cagacgctcc agcaagtgct 1080

gtgcgtattg cacaatgacg cctttttgca gcacggcatg atctgtctgt acgacagcca 1140

gcaggcgatt ttgaatattg aagcgttgca ggaagccgat cagcagttaa tccccggcag 1200

ctcgcaaatc cgctatcgtc cgggcgaagg gctggtcggg acggtgcttt cgcagggcca 1260

atcattagtg ctggcgcgcg ttgctgacga tcagcgcttt cttgaccggc tcgggttgta 1320

tgattacaac ctgccgttta tc 1342

<210> 85

<211> 1270

<212> DNA

<213> Rahnella aquatilis

<220>

<223> 16S

<220>

<221> modified base

<222> (186)..(186)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (458)..(458)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (1041)..(1041)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (1131)..(1132)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (1135)..(1135)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (1139)..(1140)

<223> a, c, t, g, unknown or other

<400> 85

attgaagagt ttgatcatgg ctcagattga acgctggcgg caggcctaac acatgcaagt 60

cgagcggcag cgggaagtag cttgctactt tgccggcgag cggcggacgg gtgagtaatg 120

tctgggaaac tgcctgatgg agggggataa ctactggaaa cggtagctaa taccgcatga 180

cctcgnaaga gcaaagtggg ggatcttcgg acctcacgcc atcggatgtg cccagatggg 240

attagctagt aggtgaggta atggctcacc taggcgacga tccctagctg gtctgagagg 300

atgaccagcc acactggaac tgagacacgg tccagactcc tacgggaggc agcagtgggg 360

aatattgcac aatgggcgca agcctgatgc agccatgccg cgtgtgtgaa gaaggcctta 420

gggttgtaaa gcactttcag cgaggaggaa ggcatcanac ttaatacgtg tggtgattga 480

cgttactcgc agaagaagca ccggctaact ccgtgccagc agccgcggta atacggaggg 540

tgcaagcgtt aatcggaatt actgggcgta aagcgcacgc aggcggtttg ttaagtcaga 600

tgtgaaatcc ccgcgcttaa cgtgggaact gcatttgaaa ctggcaagct agagtcttgt 660

agaggggggt agaattccag gtgtagcggt gaaatgcgta gagatctgga ggaataccgg 720

tggcgaaggc ggccccctgg acaaagactg acgctcaggt gcgaaagcgt ggggagcaaa 780

caggattaga taccctggta gtccacgctg taaacgatgt cgacttggag gttgtgccct 840

tgaggcgtgg cttccggagc taacgcgtta agtcgaccgc ctggggagta cggccgcaag 900

gttaaaactc aaatgaattg acggggggccc gcacaagcgg tggagcatgt ggtttaattc 960

gatgcaacgc gaagaacctt acctactctt gacatccacg gaattcgcca gagatggctt 1020

agtgccttcg ggaaccgtga nacaggtgct gcatggctgt cgtcagctcg tgttgtgaaa 1080

1140

gggaactcaa aggagactgc cggtgataaa ccggaggaag gtggggatga cgtcaagtca 1200

tcatggccct tacgagtagg gctacacacg tgctacaatg gcatatacaa agagaagcga 1260

actcgcgagg 1270

<210> 86

<211> 876

<212> DNA

<213> Rahnella aquatilis

<220>

<223> nifH

<400> 86

atggcaatgc gtcaatgcgc aatctacggg aaagggggta ttgggaaatc caccactacc 60

caaaaccttg tagcggctct ggccgaaatg aataagaagg tcatgatcgt cggctgtgac 120

cctaaggctg attcaacccg cctcattctg catgcgaaag cacagaacac catcatggaa 180

atggccgctg aagtgggctc cgtggaagat ctggagctgg aagatgtgat gcaaatcggc 240

tatggcggcg tgcgctgtgc ggaatcaggc ggccctgagc ctggtgtggg ttgtgccgga 300

cgcggggtga tcaccgccat caacttcctc gaagaagaag gcgcgtatgt gccggatctg 360

gtggtctgtg gcggtttcgc gatgccaatt 420

cgcgaaaaca aagcgcagga aatctacatc gtgtgctccg gtgaaatgat ggcgatgtat 480

gccgccaaca acatttccaa aggcatcgtg aaatacgcga aatcgggcaa agttcgcctg 540

gccgggctga tctgtaactc ccgccagacg gatcgcgaag atgaactgat catcgcgctg 600

gctgaaaaac ttggcacgca aatgatccac ttcgtgccgc gtgacaacat tgtgcaacgc 660

gctgaaatcc gccgcatgac ggtcatcgaa tacgacccga cttgtgcgca ggcagatcag 720

tatcgtgcac tggcgaacaa aatcgtcaac aacaccaaaa tggtggtgcc gacaccggtc 780

840

gccatcgtcg gtcgtaccgc cgccgaagag gcgtga 876

<210> 87

<211> 1374

<212> DNA

<213> Rahnella aquatilis

<220>

<223> nifD1

<400> 87

atgaaggcaa aagagattct ggcgctgatt gatgagccag cctgtgagca taaccacaag 60

cagaagtcgg gttgcagcct gccgaaaccg ggcgcgacgg caggcggttg tgcgtttgat 120

ggcgcgcaga ttgcgctgct gccggtcgcg gacgtcgcgc atctggtgca cggcccgatt 180

ggctgtaccg gcagttcatg ggacaaccgt ggcagccgca gttccggggcc ttccatcaac 240

cgcatgggct tcaccaccga catgagcgag caggatgtga ttatggggcg cggcgagcga 300

360

tacaacacct gcgtacccgc gatggaaggg gatgacgttg aagccgtgtg tcgcgccgca 420

tcggccgctg ccggtgtgcc ggttatttca gtcgatgccg ccggtttcta cggcagcaaa 480

aatctcggta accgcattgc cggggacgtg atggtcaaaa aggtgatcgg ccagcgcgaa 540

cccgcgccgt ggccggaaaa ctcaccgatc cccgccggac accgccacag catcagcctg 600

attggcgaat tcaatattgc cggcgagttc tggcacgttc tgccgctgct cgatgagctc 660

gggatccgcg tgctgtgcag cctttccgggg gattcccgtt ttgctgaaat ccagactatg 720

caccgtggcg aagccaacat gctggtgtgc tcgcgggcgc tgatcaacgt cgcccgaaaa 780

atggaagagc gttaccagat cccatggttt gaaggcagtt tttatggcct gcgttccatg 840

gctgattccc tgcgcacgat cgccgtgctg ctcaaagacc cggatttaca ggcgcgcaca 900

gaacgtctga ttgagcgcga ggaggcggcg acacatcttg cgcttgcgcc ttaccgtgcg 960

cggctcagcg ggcgcaaggc gctgctgtat accggtggcg tgaaatcctg gtcggtggtc 1020

tcggcgttac aggatttagg catcacggtg gtggcgaccg gcacccgaaa atcaaccgaa 1080

gaagacaagc agcgtattcg cgaactgatg ggtgaagacg tgctgatgct cgacgaaggc 1140

aatgccagaa ccttgctcga caccctctat cgtttcggcg gcgacatcat gatcgccggg 1200

ggccgcaaca tgtataccgc gtacaaagcc cgcctgccgt tcctggatat caatcaggag 1260

cgcgagcatg cgtttgccgg atatcacggg ctggtaaatc tggccgaaca gttgtgtatc 1320

accctggaaa gcccggtctg ggcgcaggtc aaccgtctgg cgccgtggcg ctaa 1374

<210> 88

<211> 1449

<212> DNA

<213> Rahnella aquatilis

<220>

<223> nifD2

<400> 88

atgaccagtg aaacacgcga acgtaacgag gcattgatcc aggaagtgct ggagatcttc 60

cccgagaagg cgcttaaaga tcgtaagaaa cacatgatga ccaccgaccc ggcgatggaa 120

tctgtcggca agtgtattgt ctcaaaccgc aaatcacagc cgggcgtgat gaccgtgcga 180

ggctgcgctt acgccggttc caaaggcgtg gtctttggcc cgatcaaaga catggcgcat 240

atctccccg gcccggttgg ttgcggccag tattcccgtg ccggacgccg taactattac 300

accggctgga gcggcgtgaa cagctttggc accctcaact tcaccagtga ttttcaggaa 360

cgggacatcg tatttggcgg cgataaaaag ctcgacaaat tgatcgatga actggagatg 420

ttgttcccgc tgagcaaagg catttcggtg cagtcggaat gtccggtcgg tctgatcggc 480

gatgacattt ctgccgtcgc caaagccagc agcgccaaaa tcggtaagcc ggtcgtgccg 540

gtacgctgcg aggggttccg cggtgtgtcg caatcgctcg gccatcacat tgctaacgat 600

gtcatccgcg actgggtgct ggataaccgc gaaggcaatg aatttgaaac cacgccttac 660

gacgtggcga ttatcggcga ctacaacatc ggcggtgacg cctgggcctc acgtattctg 720

ctcgaagaaa tggggctgcg cgtggtggcg cagtggtccg gcgacggcac gctggtggag 780

atggaaaaca ccccgaaagt cgcgctcaat ctggtgcact gctaccgctc gatgaactac 840

atctcccgtc atatggaaga aaaacacggc attccgtgga tggaatacaa cttctttggc 900

ccgaccaaaa ttgcggaatc tctgcgcgaa atcgcggcgc gttttgacga taccatccgg 960

aaaaacgccg aagcggtgat tgaaaaatat caggcgcaaa cgcaggcggt gatcgacaaa 1020

taccgtccgc gtctggaagg caaaaaggtg ctgttgtatc tcggcggttt acgtccgcgc 1080

cacatcatcg gggcgtatga agatctggga atggaaatca tcggtaccgg ctatgaattc 1140

ggtcataacg atgattacga ccgcacctta ccgatgctca aagaaggcac gttgctgttc 1200

gatgacctga gcagttatga gctggaagcg ttcgttaaag cgctgaaacc ggatcttgtc 1260

gggtcaggta tcaaagaaaa atacattttc cagaaaatgg gcgtgccgtt ccgccagatg 1320

1380

gacatggaca tgacgctgaa caatccgggc tggagtcagc tgaccgcccc ctggttgaaa 1440

acggcctga 1449

<210> 89

<211> 1386

<212> DNA

<213> Rahnella aquatilis

<220>

<223> nifK1

<400> 89

atggctcaaa ttctgcgtaa tgccaagccg cttgccacca cgcctgtcaa aagcgggcaa 60

ccgctcgggg cgatcctggc cagtcagggg ctggaaaatt gcatcccgct ggttcacggc 120

gcgcaaggtt gtagcgcgtt cgccaaagtt ttcttcatcc agcattttca cgatccgatc 180

ccgttgcagt ccacggcgat ggaatcgacc acgactatca tgggctcgga tggcaacgtc 240

agtactgcgt tgaccacgtt gtgtcagcgc agtaatccaa aagccattgt gattttgagc 300

accggactgt cagaagcgca gggcagtgat ttgtcgatgg cgctgcgtga gtttcgcgac 360

aaagaaccgc gctttaatgc catcgctatt ctgaccgtta acacgccgga ttttacggc 420

tcgctggaaa acggctacag cgcgctgatg gaaagcgtga tcactcagtg ggtgccggaa 480

aagccgccga ccggcatgcg taacaagcgc gtgaacctgc tggtgagcca tctgctgacg 540

ccgggggatc tggaattact gcgcagctat gtcgaagcct ttggcctgca accggtgatc 600

ctgccggatt tatcacagtc gctggacggga catctggcga atggcgattt caatccggtc 660

acgcagggcg gcacgtcgca acgccagatt gaacaaatgg ggcagagcct gaccaccatt 720

accattggca gttcgctcaa ctgcgccgcc agtctgatgg cgatgcgcag ccgtggcatg 780

gcgctgaacc tgccgcacct gatgacgctg gaaaacatgg acagtctgat ccgccatctg 840

catcaggtgt caggccgcga ggtaccggca tggattgagc gccagcgcgg gcaactgctg 900

gacgccatga tcgactgcca tacctggctg cagtcacagc gtattgcgct ggcggcagaa 960

gcggatttgc tggtggcgtg gtgcgatttc gctcagagcc agggaatgcg cgtcgggccg 1020

gtgattgcgc cggttaatca gcagtcactg gccgggctgc cggtcgaaca ggtggtgatc 1080

ggcgatctgg aagatttaca aacccggctc gacagctacc cggtttcact gctggtggcg 1140

aactccccg ctgcaccact ggcggaaaaa aacggtatcg cgctggtacg tgccggtttc 1200

ccgctttacg accgtctcgg ggaatttcgc cgcgtgcggc agggctatgc gggtattcgc 1260

gacaccttgt tcgaactcgc gaacctgatg caggcgcgcc atcacatgct gacggcgtat 1320

cactcaccgc ttaggcaggt gttcggcctg agcccggtac cggaggccag tcatgaggcg 1380

cgctaa 1386

<210> 90

<211> 1569

<212> DNA

<213> Rahnella aquatilis

<220>

<223> nifK2

<400> 90

atgagtcaag atcttggcac cccaaaatcc tgtttcccgc tgttcgagca ggatgaatac 60

cagaatatgt ttacccacaa acgcgcgctg gaagaagcac acggcgaggc gaaagtgcgg 120

gaagtgtttg aatggaccac cacgcaggaa tatcaggatc tgaacttctc gcgtgaagcg 180

ctgaccgtcg acccggcgaa agcctgccag ccgttaggcg cggtactttg cgcgctgggt 240

tttaccaaca cgttgccgta tgtccatggt tcacaaggct gtgtggcgta tttccgtacc 300

360

gccgccgttt ttggcggaaa taacaacatg aatgtcggtc tggaaaacgc cagcgcgctg 420

tacaagccgg aaattattgc ggtctccacc acctgtatgg cggaagtgat cggtgatgac 480

ctgcaggctt ttatcgccaa cgccaaaaaa gacggatttg tggatgccgg tatgccaatc 540

ccgtatgccc atacaccgag ttttctgggc agtcatgtca ccggctggga caacatgttt 600

gaaggcttcg cccgtacctt taccaccgac gccacgcggg aatatcagcc gggcaaactt 660

gccaaactga acgtggtgac cggttttgaa acttatctcg gcaactaccg ggttattcac 720

cgcatgatga gccagatggg ggtcgaatgc agcgtcttgt ccgatccgtc tgaagtgctc 780

gacaccccgg ctgacggcca ataccgcatg tatgccggcg gcaccacgca aaccgaaatg 840

cgtgatgcac cggatgccat cgacaccttg ctgctgcaac cgtggcaatt acagaaaacc 900

aaaaaggtgg tgcagggcga ctggaatcag ccgggcaccg aagtcagtgt accgattggc 960

ctggcggcga ccgatgcctt gctgatgacg gtaagcgaac tgaccggcaa accgatagct 1020

1080

ctgcacggca agcgtttcgg tctctacggt gacccggatt ttgtgatgggg catgaccgca 1140

ttcctgctgg aactgggctg tgaaccgacc accattctca gccataacgg caacaaacgc 1200

tggcagaaag ccatgaagaa aatgctggct gattcgcctt acggacagga cagcgaagtg 1260

tatgtgaact gcgatctgtg gcatttccgc tcgctgatgt ttacccgtaa accggacttt 1320

atgatcggca actcttacgg aaaattcatt cagcgtgaca cgctggccaa aggcgaacag 1380

ttcgaagtgc cgctgatccg tatcggattc ccgatttttg accggcacca tttgcaccgt 1440

cagaccacct ggggatacga gggcgcgatg agcatcctga cgcaactggt gaatgcggtg 1500

1560 ctcgaacagc tggatcgcga aaccatgaag ctcggcaaaa

atccgctaa 1569

<210> 91

<211> 1488

<212> DNA

<213> Rahnella aquatilis

<220>

<223> nifL

<400> 91

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat catcggtggc gatttcgctg 120

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 180

240

catatctatg acgaaatgtg gcgcactttg ttgcaggggca aatcctggaa cggccaactg 300

atcaaccggc gtaataaccg ttcgctttat ctggcggatg tcactatcac gcctgtttta 360

ggcgcggacg ggcaggtgga gcattacctc ggcatgcaca aagatatcag cgagaaatac 420

gcgctggagc agcggttgcg caaccacatc accttgttca cggaggtgct gaacaatatt 480

cccgccgccg tggtggtggt ggatgagcag gacaatgtgg tgatggacaa tctggcctac 540

aaaaccctgt gcgctgactg cggcggaaaa gagctgttgg ccgaaatggg ctatccgcaa 600

ctcaaagaga tgctcaacag tggcgaaccg gtgccggttt ccatgcgcgg caacgtacgc 660

tggttttctt tcggtcagtg gtcattgcag ggcgttaatg aagaggccag ccgctttttt 720

accggcatta ccgcgccggg aaaactgatt gttctcaccg actgcaccga tcagcatcac 780

cggcagcagc agggttatct tgaccggctc aagcaaaaac ttaccaacgg caaattgctg 840

gcagccatcc gcgagtcgct tgatgccgcg ctgattcagc tcaacgggcc aattaatatg 900

ctggcggctg cgcgtcgtct taacggcgaa gaaggcaaca acatggcgct ggaattcgcc 960

tggcgcgaag gcgagcaggc ggtgagtcgc ttacaggcct gccgtccgtc gctggatttt 1020

gagccgcagg cagaatggcc ggtcagtgaa ttcttcgacg atctgagcgc gctgtacgac 1080

agccattttc tcagtgacgg tgaattgcgt tacgtggtca tgccatctga tctgcacgct 1140

gtcgggcaac gaacgcaaat ccttaccgcg ctgagcttat ggattgatca cacgctgtca 1200

caggcgcagg ccatgccgtc tctgaagctc tcggtgaaca ttgttgcgaa gcaggatgcg 1260

agctggttgt gttttgacat taccgataat gtgccgcgtg aacgggtgcg ttatgcccgc 1320

ccggaagcgg cgttttcccg tccggggaat ggcatggagc tgcgccttat cggacgctg 1380

atcgcccatc atcgcggttc tttagatctc tcggtccgcc ctgatggcgg caccttgctg 1440

acgttacgcc tgccggtaca gcaggttatc accggaggct taaaatga 1488

<210> 92

<211> 1557

<212> DNA

<213> Rahnella aquatilis

<220>

<223> nifA

<400> 92

atgacccagt tacctaccgc gggcccggtt atccggcgct ttgatatgtc tgcccagttt 60

acggcgcttt atcgcatcag cgtggcgctg agtcaggaaa gcaataccgc gcgcgcactg 120

gcggcgatcc tcgaagtgct tcacgatcat gcatttatgc aatacggcat ggtgtgtctg 180

ttcgataaag aacgcaatgc actgtttgtg gaatccctgc atggcatcga cggcgaaagg 240

aaaaaagaga cccgccatgt ccgttaccgc atgggggaag gcgtgatcgg cgcggtgatg 300

agccagcgtc aggcgctggt gttaccgcgc atttcagacg atcagcgttt tctcgaccgc 360

ctgaatattt acgattacag cctgccgctg attggtgtgc cgatccccgg tgcggataat 420

cagcctgcgg gtgtgctggt ggcacagccg atggcgttgc acgaagaccg gctggctgcc 480

agtacgcggt ttttagaaat ggtcgccaat ctcatcagcc agccactgcg ttctgccacg 540

ccccgggaat cattgcctgc tcaaacgccg gtccggtgca gtgttccgcg ccagtttggt 600

tttgagcaga tggtcgggaa aagtcaggcg atgcgccaga cgatggacat tttacggcag 660

gtttccaaat gggataccac ggttctggtg cgtggtgaaa gcggcaccgg caaggaactt 720

atcgccaatg ccattcatta caactcaccc cgtgcggccg cgccatttgt gaaattcaac 780

tgcgccgcgc tgccggataa cctgctggaa agcgaactgt tcggtcatga aaaaggggcc 840

ttcaccggcg ctatacgcac ccgaaaaggc cgctttgaac tggcggacgg gggcacgtta 900

ttcctcgatg aaatcggcga atcgagcgcg tcgtttcagg ccaaattgct gcgcattttg 960

caggaaggtg aaatggaacg ggtcggcggc gataccacgc tgaaagttga tgtgcgcatt 1020

1080 attgctgcca ccaaccgtaa tcttgaggag gaagtgcgtg

ctgtattatc gcctgaacgt gatgccggtt tcgctgcctg cactgcgtga aaggctggat 1140

gatatcgccg atctggcgcc gtttctggtc aaaaagattg cgctgcgtca ggggcgggaa 1200

ctgcgcatca gtgatggtgc ggtgcgtctg ctgatgacct acagctggcc aggcaacgtg 1260

cgtgaactgg aaaactgcct cgaacgggcg tcggtaatga ccgatgaagg gctgatcgac 1320

cgcgacgtga tcctgttcaa tcaccatgag tccccggcgc tgtccgtcaa acccggtctg 1380

ccgctggcga cagatgaaag ctggctggat caggaactcg acgaacgcca gcgggtgatt 1440

gctgcactgg agaaaaccgg ctgggtgcag gccaaagcgg cccgactgct gggcatgaca 1500

ccgcgccaga ttgcctaccg tatccagatt atggacatca acatgcaccg tatctga 1557

<210> 93

<211> 2838

<212> DNA

<213> Rahnella aquatilis

<220>

<223> glnE

<400> 93

atgttgccac tttcttctgt tttgcaaagc cacgcgcaga gtttgcctga acgctggcat 60

gaacatcctg aaaacctgcc cctccccgat gatgaacagc tggctgtgct gagcagcagt 120

gaattcatga cggacagttt gctggctttt ccgcagtggt ggcatgaaat tgtccaaaat 180

ccccctcagg cgcaggagtg gcaactttac cgtcagtggc tggatgaatc gctgacgcag 240

gtgactgacg aagccgggtt aatgaaagct ttgcgtctgt tccgccgccg tattctgacc 300

cgcattgcgt ggtcacagtc cgcgcaaacc agcgaagcaa aagatacgct tcaccagctg 360

agtgaactgg cggaattatt gattgtcagc gcccgtgact ggctgtatgc cgcttgctgt 420

cgcgagttcg gtacgccggt caatgccgca ggggaaccgc agagaatgct gatcctcggg 480

atgggcaaac tcggcggtgg cgagctgaat ttctcatcgg acatcgacct gatttttgct 540

tatccggaaa atggccagac acgcggcggt cggcgtgaac tggataacgc acaatttttc 600

acccggctcg gccagcgtct gatcaaagcg ctggatcagc ccactatcga cggttttgtc 660

tatcgcgtgg acatgcgttt gcgtccgttc ggcgacagtg gcccgctggt gatgagcttc 720

ccggcactgg aagattatta tcaggaacag gggcgcgact gggaacgcta cgcaatggtg 780

aaagcgcgtc tgatgggcgg cgcgggaggac atcagcagtc aggaattgcg taaaatgctg 840

atgccttttg tcttccgccg ttatatcgat ttcagtgtga tccagtccct gcgtaacatg 900

aaaggcatga tcgcccgcga agtacgccgc cgtggtctga aagacaacat caaactcggc 960

gcaggcggta ttcgtgaaat tgaatttatc gtgcaggtat ttcagctgat ccgtggcggt 1020

cgtgaaccgg cattgcagca gcgtgcgttg ttgccaacgc ttcaggcgct ggaaaatctg 1080

gggctgctgc cggtagagca ggtgttgcag ttgcgtaaca gctatctgtt cctgcgacgt 1140

ctggaaaacc tgttgcaggc cattgctgac gagcaaacgc aaaccttacc gtccgatgag 1200

ctgaatcagg cgcgtctggc gtgggggatg aattacgctg gctggccgca gcttctggat 1260

gcagtgaatg ctcacatgca ggccgtacgc gcggtattta acgatctgat tggcgatgac 1320

acgccagatg ccgaagatga cgtgcaactc tcccggttca gcagtttatg gattgatacg 1380

cttgagcctg acgagctggc tccgctggtg ccgcaacttg acgaaaatgc gcaacggcat 1440

gttttacatc agattgctga ttttcgccgt gacgtggata aacgcacgat agggccacgt 1500

gggcgtgatc agttggattt gctgatgccg cgtttactgg cccaggtctg cacctataaa 1560

aatgcggatg tgacgttaca gcgcctgatg cagttgctgc tcaatatcgt cacgcgcacg 1620

acgtatatcg agctgctggt ggaatatccc ggtgcgctca aacagttaat acgtctgtgc 1680

gctgcctcgc cgatggtggc gacgcaactt gcgcgtcatc ctttattgct cgacgaactg 1740

ctcgacccgc gcacgcttta ccagccgatt gagccgggcg cgtaccgtga tgaactgcgg 1800

caatatctga tgcgggtgcc aaccgaagac gaagaacaac agcttgaagc cgtgcgccag 1860

ttcaaacagg cacagcattt acgtattgcg gccggggata tttccggtgc gttgccggtg 1920

atgaaagtca gtgaccattt aacctacctt gcggaggcca ttctcgacgt tgtggtgcaa 1980

caggcgtggg aacaaatggt cgtaaaatac ggtcagccaa cccatcttca gcaccgtaaa 2040

gggcgcggtt ttgccgtggt gggttacgga aaactcggtg gctgggagct gggttacagc 2100

tcggatctgg atctggtctt cctgctcgat tgcgcgccgg aagtcatgac cgacggcgaa 2160

cgcagcattg acgggcgtca gttttatctg cggctggcgc agcgcatcat gcatttattc 2220

agcacccgta cgtcgtcagg cattctttat gaggttgacc cgcgtctgcg gccttccggt 2280

gcttccggca tgctggtcag caccatcgaa gcttttgcgg attatcaggc caacgaagcc 2340

tggacatggg agcatcaggc gctggttcgc gcgcgtgtgg tttatggtga tccgcaactg 2400

acgcagcaat ttaatgccac gcgtcgcgac attctttgcc gccagcgcga tgccgacggc 2460

ttgcgtaagg aagtccgtga aatgcgcgag aaaatgtatg cccatctggg cagcaaaaga 2520

gccgacgagt ttgatctgaa agccgatccg ggtggcataa cggatattga attcatcgca 2580

caatatctgg ttctgcgttt cgcgcatgat gagccgaagc tgacccgctg gtctgataac 2640

gtgcggattt tcgaactgat ggcgcgacat gacatcatgc cggaagagga agcacgccat 2700

ctgacgcagg cttacgtgac attgcgcgat gaaattcatc atctggcgtt gcaggaacac 2760

agcgggaaag tggccgcaga cagctttgcc actgagcgcg cgcaaatccg cgccagctgg 2820

gcaaactggc ttggctga 2838

<210> 94

<211> 1290

<212> DNA

<213> Rahnella aquatilis

<220>

<223> amtB

<400> 94

atgaaaaaac ttttatccat gatggggctt ggtgcagtgg ctttgctacc ttcgcttgcc 60

atggcagcag caccagcagc ggcaaacggt gctgataacg cctttatgat gatttgtacc 120

gcgctggtat tgttcatgac cgtacccggt gtggcgttgt tctacggcgg cttactgcgt 180

tctaaaaacg ttttgtccat gctgactcag gttattgtta cctttgctct ggtctgcgtc 240

ctgtggatcc tctacggtta cagccttgcc ttcagtgaag gtaacgcgtt cttcggtggt 300

ttcagcaacg taatgatgaa aggcattggc ctggattctg tgactggcac cttctcgcag 360

atgatccacg ttgcattcca gtgttcattt gcctgcatca ctgtagcgct gatcgtaggt 420

ggtattgctg aacgtgtgcg tttctcagca gttctgattt tcactgtgat ctggctgact 480

ttctcttata ttccgatggc tcacatggta tgggcaggcg gtttcctggc tgctgacggt 540

gcgctggact ttgccggtgg taccgttgtt catatcaatg ccgcaattgc tggcctggta 600

ggggcttatc tgctgggtaa acgcgccggt tttggcaaag aagctttcaa accacacaac 660

ctgccaatgg tcttcactgg cgcctcaatc ctgtatgtgg gctggttcgg cttcaatgcg 720

ggttcagcaa gtgccgcaag ctctgttgcc gcgctggctt tcctgaacac tgtcattgct 780

actgctggcg caatcctgtc ctggacgctg gttgagtgga tggtgcgcgg taagccctca 840

ctgctgggcg caagctccgg tgctatcgca ggtctggtgg ctatcacgcc tgcatgtggt 900

acggtcggcg taggtggtgc tctgattatc ggtctggtag gcggtatcac tggtctgtgg 960

ggggttgtta ccctgaaaaa atggctgcgt gttgatgaca cctgtgatgt gttcggtgtt 1020

catggcgtgt gcggtatcgt aggttgtctg ctgacgggtg tattcacgtc cagttcactt 1080

ggcggcgtgg gctacgcaga aggcgtgacc atgggccatc aggtttgggt gcagttcttc 1140

agcgtgtgcg taacattggt ctggtcaggc gttgttgcct tcatcggtta caaagtggct 1200

gacatgatcg taggtctgcg tgttcctgaa gaacaagaac gcgaaggtct ggacgttaac 1260

agccacggcg aaaacgctta caaccaataa 1290

<210> 95

<211> 500

<212> DNA

<213> Rahnella aquatilis

<220>

<223> PinfC

<400> 95

tgaatatcac tgactcacaa gctacctatg tcgaagaatt aactaaaaaa ctgcaagatg 60

caggcattcg cgttaaagcc gacttgagaa atgagaagat tggctttaaa attcgcgaac 120

acacgctacg ccgtgttcct tatatgttag tttgtggcga taaagaggtc gaagcaggca 180

aagttgctgt tcgtactcgt cgcggcaaag acttaggaag catggatgtt agcgaagtcg 240

ttgacaaact gctggcggaa atccgcagca gaagtcatca tcaactggag gaataaagta 300

ttaaaggcgg aaaacgagtt caaccggcgc gtcctaatcg cattaacaaa gagattcgcg 360

cgcaagaagt tcgcctcacc ggcgtcgatg gcgagcagat tggtattgtc agtctgaatg 420

aagctcttga aaaagctgag gaagcgggcg tcgatttagt agaaatcagt ccgaatgccg 480

agccgccagt ttgtcgaatc 500

<210> 96

<211> 1085

<212> DNA

<213> Klebsiella variicola

<220>

<223> 16S

<400> 96

attgaagagt ttgatcatgg ctcagattga acgctggcgg caggcctaac acatgcaagt 60

cgagcggtag cacagagagc ttgctctcgg gtgacgagcg gcggacgggt gagtaatgtc 120

tgggaaactg cctgatggag ggggataact actggaaacg gtagctaata ccgcataacg 180

tcgcaagacc aaagtggggg accttcgggc ctcatgccat cagatgtgcc cagatgggat 240

tagctagtag gtggggtaac ggctcaccta ggcgacgatc cctagctggt ctgagaggat 300

gaccagccac actggaactg agacacggtc cagactccta cgggaggcag cagtggggaa 360

420

gttgtaaagc actttcagcg gggaggaagg cggtgaggtt aataacctca ccgattgacg 480

ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat acggagggtg 540

caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtctgtc aagtcggatg 600

tgaaatcccc gggctcaacc tgggaactgc attcgaaact ggcaggctag agtcttgtag 660

aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg aataccggtg 720

gcgaaggcgg ccccctggac aaagactgac gctcaggtgc gaaagcgtgg ggagcaaaca 780

ggattagata ccctggtagt ccacgctgta aacgatgtcg atttggaggt tgtgcccttg 840

aggcgtggct tccggagcta acgcgttaaa tcgaccgcct ggggagtacg gccgcaaggt 900

taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga 960

tgcaacgcga agaaccttac ctggtcttga catccacaga actttccaga gatggattgg 1020

tgccttcggg aactgtgaga caggtgctgc atggctgtcg tcagctcgtg ttgtgaaatg 1080

ttggg 1085

<210> 97

<211> 882

<212> DNA

<213> Klebsiella variicola

<220>

<223> nifH1

<400> 97

atgaccatgc gtcaatgcgc tatctacggt aaaggcggta tcggtaaatc caccaccacc 60

cagaatctcg tcgcggccct cgccgagatg ggtaagaaag tgatgatcgt cggctgcgat 120

ccgaaagcgg attccacccg tctgatcctc cacgctaaag cccagaacac catcatggag 180

atggcggcgg aagtgggctc ggtcgaggat ctggagctcg aagacgttct gcaaatcggc 240

tatggcgatg tccgttgcgc cgaatccggc ggcccggagc caggcgtcgg ctgcgccgga 300

360

gatttcgtct tctatgacgt cctcggcgac gtggtctgcg gcggcttcgc tatgccgatc 420

cgcgaaaaca aagccgga gatctacatc gtctgctccg gcgagatgat ggcgatgtat 480

gccgccaaca atatctccaa agggatcgtg aagtacgcca aatccggcaa ggtgcgcctc 540

ggcggcctga tctgtaactc gcgcaaaacc gaccgggaag acgaactgat catcgccctg 600

gcggagaagc ttggcacgca gatgatccac ttcgttcccc gcgacaacat tgtgcagcgc 660

gcggagatcc gccggatgac ggtgatcgag tacgacccga cctgtcagca ggcgaatgaa 720

tatcgtcaac tggcgcagaa gatcgtcaat aacaccaaaa aagtggtgcc gacgccgtgc 780

accatggacg agctggaatc gctgctgatg gagttcggca tcatggaaga agaagacacc 840

agcatcattg gtaaaaccgc cgctgaagaa aacgcggcct ga 882

<210> 98

<211> 1113

<212> DNA

<213> Klebsiella variicola

<220>

<223> nifH2

<400> 98

atggttagga aaagtagaag taaaaataca aatatagaac taactgaaca tgaccattta 60

ttaataagtc aaataaaaaa gcttaaaaca caaaccactt gcttttttaa taataaagga 120

ggggttggga agactacatt agtagcaaat ttaggagcag agctatcaat aaactttagt 180

gcaaaagttc ttattgtgga tgccgaccct caatgtaatc tcacgcagta tgtattaagt 240

gatgaagaaa ctcaggactt atatgggcaa gaaaatccag atagtattta tacagtaata 300

agaccactat cctttggtaa aggatatgaa agtgacctcc ctataaggca tgtagagaat 360

ttcggttttg acataattgt cggtgaccct agacttgctt tacaggaaga ccttttagct 420

ggagactggc gagatgccaa aggcggtggg atgcgaggaa ttaggacaac ttttgtattt 480

gcagagttaa ttaagaaagc tcgtgagcta aattatgatt ttgttttctt tgacatggga 540

ccatcattag gcgcaatcaa cagggcagta ttactggcaa tggaattctt tgtcgtccca 600

atgtcaatcg atgtattttc actatgggct attaaaaata ttggctccac ggtttcaata 660

tggaaaaaag aattagacac agggattcgg ctctcagagg aacctagcga attatcacaa 720

ttatcacctc aaggaaaact aaagtttctc ggttacgtca cccaacaaca taaagaacgc 780

tctggatacg atacaattca gcttgagaat actgaggaag aaataaaatc gaaacgtcgg 840

gtaaaggcgt atgaagacat tggagaggtg tttccttcta aaattactga gcatctttct 900

aaactttatg catcaaaaga tatgaaccca caccttggag atatacgtca tttaggtagt 960

ttagctccga aatcacaatc acaacacgtt ccgatgatat cagtgtctgg tacaggaaat 1020

tacaccagac ttagaaaaag cgcgcgtgaa ctttatcgag atattgcaag aagatactta 1080

gagaacattc agactgctaa tggcgagaaa tag 1113

<210> 99

<211> 1374

<212> DNA

<213> Klebsiella variicola

<220>

<223> nifD1

<400> 99

atgaagggaa aggaaattct ggcgctgctg gacgaacccg cctgcgagca caaccagaag 60

caaaaatccg gctgcagcgc ccctaagccc ggcgctaccg ccggcggttg cgccttcgac 120

ggcgcgcaga taacgctcct gcccatcgcc gacgtcgcgc acctggtgca cggccccatc 180

ggctgcgcgg gcagctcgtg ggataaccgc ggcagcgtca gcgccggccc ggccctcaac 240

cggctcggct ttaccaccga tcttaacgaa caggatgtga ttatggggccg cggcgaacgc 300

cgcctgttcc acgccgtgcg tcacatcgtc gaccgctatc atccggcggc ggtctttatc 360

tacaacacct gcgtaccggc gatggagggc gatgacatcg aggcggtctg ccaggccgca 420

cagaccgcca ccggcgtccc ggtcatcgct attgacgccg ccggtttcta cggcagtaaa 480

aatcttggca accgaatggc gggcgacgtg atgctcaggc aggtgattgg ccagcgcgaa 540

ccggccccgt ggccagacaa cacgcccttt gccccggccc agcgccacga tatcggcctg 600

attggcgaat tcaatatcgc cggcgagttc tggcaggtcc agccgctgct cgacgagctg 660

gggatccgcg tcctcggcag cctctccggc gacggccgct ttgccgagat ccagaccctg 720

caccgggcgc aggccaatat gctggtgtgc tcgcgcgcgc tgatcaacgt cgcccggggg 780

ctggagctgc gctacggcac gccgtggttt gaaggcagct tctacgggat ccgcgccacc 840

tccgacgcct tgcgccagct ggcgacgctg ctgggggatg acgacctgcg ccgccgcacc 900

gaggcgctga tcgcccgcga agagcaggcg gcggagcagg ctcttgcgcc gtggcgtgag 960

cagctccgcg ggcgcaaagt gctgctctat accggcggcg tgaaatcctg gtcggtggta 1020

tcggccctgc aggatctcgg catgaccgtg gtggccaccg gcacgcgcaa atccaccgag 1080

gaggacaaac agcggatccg tgagctgatg ggcgacgagg cggtgatgct tgaggagggc 1140

aatgcccgca ccctgctcga cgtggtgtac cgctatcagg ccgacctgat gatcgccggc 1200

ggacgcaata tgtacaccgc ctggaaagcc cggctgccgt ttctcgatat caatcaggag 1260

cgcgagcacg cctacgccgg ctatcagggc atcatcaccc tcgcccgcca gctctgtctg 1320

accctcgcca gccccgtctg gccgcaaacg catacccgcg ccccgtggcg ctag 1374

<210> 100

<211> 1449

<212> DNA

<213> Klebsiella variicola

<220>

<223> nifD2

<400> 100

atgaccaacg caacaggcga acgtaacctt gcgctcatcc aggaagtcct ggaggtgttt 60

cccgaaaccg cgcgcaaaga gcgcagaaag cacatgatga tcagcgatcc gcagatggag 120

agcgtcggca agtgcattat ctcgaaccgt aaatcgcagc ccggggtgat gaccgtgcgc 180

ggctgcgcct atgcgggctc gaaaaggggtg gtgtttgggc caatcaaaga catggcccat 240

atctcgcacg gccccatcgg ctgcggccag tattcccgcg ccggacggcg caactactat 300

accggcgtca gcggtgtcga cagcttcggc accctgaact tcacctctga ttttcaggag 360

cgcgatattg ttttcggcgg cgataaaaag ctgaccaaac tgatcgaaga gatggagctg 420

ctgttcccgc tgaccaaagg gatcaccatc cagtcggagt gcccggtggg cctgatcggc 480

gatgacatca gcgccgtagc caacgccagc agcaaggcgc tggataaacc ggtgatcccg 540

gtgcgctgcg aaggctttcg cggcgtatcg caatcgctgg gccaccatat cgccaacgac 600

gtggtgcgcg actgggtgct gaacaatcgc gaagggcagc cgtttgccag caccccgtac 660

gatgttgcca tcattggcga ttacaacatc ggcggcgacg cctgggcctc gcgcattctg 720

ctggaagaga tggggctgcg cgtagtggcg cagtggtccg gcgacggcac cctggtggag 780

atggagaaca ccccattcgt taagcttaac ctcgtccact gctaccgttc gatgaactat 840

atcgcccgcc atatggagga gaaacatcag atcccatgga tggaatataa cttcttcggc 900

ccgaccaaaa tcgccgaatc gctgcgcaag atcgccgatc aatttgatga caccattcgc 960

gccaatgcgg aagcggtgat cgccaaatat gaggggcaga tggcggccat catcgccaaa 1020

tatcgcccgc ggctggaggg gcgcaaagtg ctgctgtaca tgggggggct gcggccgcgc 1080

cacgtcatcg gcgcctatga ggatctcggg atggagatca tcgccgccgg ctacgagttt 1140

gcccataacg atgattacga ccgcaccctg ccggacctga aagagggcac cctgctgttt 1200

gacgatgcca gcagctatga gctggaggcc ttcgtcaaag cgctgaaacc tgacctcatc 1260

ggctccggga tcaaagagaa atatatcttc cagaaaatgg gggtgccgtt ccgccagatg 1320

cactcctggg actattccgg cccctatcac ggctatgacg gcttcgccat ctttgcccgc 1380

gatatggata tgaccctgaa caatccggcg tggaacgaac tgactgcccc gtggctgaag 1440

tctgcgtga 1449

<210> 101

<211> 1386

<212> DNA

<213> Klebsiella variicola

<220>

<223> nifK1

<400> 101

atggcagata ttatccgcag tgaaaaaccg ctggcggtga gcccgattaa aaccgggcaa 60

ccgctcgggg cgatcctcgc cagcctcggg ctggcccagg ccatcccgct ggtcccggc 120

gcccagggct gcagcgcctt cgccaaagtt ttcttttattc agcatttcca tgacccggtg 180

ccgctgcagt cgacggccat ggatccgacc gccacgatca tgggggccga cggcaatatc 240

ttcaccgcgc tcgacaccct ctgccagcgc cacagcccgc aggccatcgt gctgctcagc 300

accggtctgg cggaagcgca gggcagcgat atcgcccggg tggtgcgcca gtttcgcgag 360

gcgcatccgc gccataacgg cgtggcgatc ctcaccgtca ataccccggga tttttttggc 420

tctatggaaa acggctacag cgcggtgatc gagagcgtga tcgagcagtg ggtcgcgccg 480

acgccgcgtc cggggcagcg gccccggcgg gtcaacctgc tggtcagcca cctctgttcg 540

ccaggggata tcgaatggct gggccgctgc gtggaggcct ttggcctgca gccggtgatc 600

ctgccggacc tctcgcagtc aatggatggc cacctcggtg aaggggattt tacgcccctg 660

acccagggcg gcgcctcgct gcgccagatt gcccagatgg gccagagtct gggcagcttc 720

gccattggcg tgtcgctcca gcgggcggca tcgctcctga cccaacgcag ccgcggcgac 780

gtgatcgccc tgccgcatct gatgaccctc gaccattgcg atacctttat ccatcagctg 840

gcgaagatgt ccggacgccg cgtaccggcc tggattgagc gccagcgtgg ccagctgcag 900

gatgcgatga tcgactgcca tatgtggctt cagggccagc gcatggcgat ggcggcggag 960

ggcgacctgc tggcggcgtg gtgtgatttc gcccgcagcc aggggatgca gcccggcccg 1020

ctggtcgccc ccaccagcca ccccagcctg cgccagctgc cggtcgagca agtcgtgccg 1080

ggggatcttg aggatctgca gcagctgctg agccaccaac ccgccgatct gctggtggct 1140

aactctcacg cccgcgatct ggcggagcag tttgccctgc cgctgatccg cgtcggtttt 1200

cccctcttcg accggctcgg tgagtttcgt cgcgtccgcc aggggtacgc cggtatgcga 1260

gatacgctgt ttgaactggc caatctgctg cgcgaccgcc atcaccacac cgccctctac 1320

1380

cattaa 1386

<210> 102

<211> 1563

<212> DNA

<213> Klebsiella variicola

<220>

<223> nifK2

<400> 102

atgagccaaa cgatcgataa aattcacagc tgttatccgc tgtttgaaca ggatgaatac 60

cagaccctgt tccagaataa aaagaccctt gaagaggcgc acgacgcgca gcgtgtgcag 120

gaggtttttg cctggaccac caccgccgag tatgaagcgc tgaacttcca gcgcgaggcg 180

ctgaccgtcg acccggccaa agcctgccag ccgctcggcg ccgtactctg cgcgctgggg 240

ttcgccggca ccctgccta cgtgcacggc tcccagggct gcgtcgccta ttttcgcacc 300

tactttaacc gccattttaa agagccggtc gcctgcgtct ccgactccat gaccgaggac 360

gcggcggtgt tcggcggcaa caacaacatg aatctgggcc tgcagaatgc cagcgcgctg 420

tataaacccg agattatcgc cgtctccacc acctgtatgg ccgaggtgat cggcgacgat 480

ctgcaggcgt ttatcgccaa cgccaaaaaa gagggatttg ttgacgaccg catcgccatt 540

ccttacgccc atacccccag ctttatcggc agccatgtca ccggctggga caatatgttc 600

660

ctcaatctgg tgaccggatt tgagacctat ctcggcaact tccgcgtgct gaagcggatg 720

atggcgcaga tggatgtccc gtgcagcctg ctctccgacc catcagaggt gctcgacacc 780

cccgccgacg gccattaccg gatgtacgcc ggcggcacca gccagcagga gatcaaaacc 840

gcgccggacg ccattgacac cctgctgctg cagccgtggc agctggtgaa aagcaaaaag 900

gtggttcagg agatgtggaa ccagcccgcc accgaggtgg ccgttccgct gggcctggcc 960

gccaccgacg cgctgctgat gaccgtcagt cagctgaccg gcaaaccgat cgccgacgct 1020

ctgaccctgg agcgcggccg gctggtcgac atgatgctgg attcccacac ctggctgcat 1080

ggcaaaaaat tcggcctcta cggcgatccg gatttcgtga tggggctgac gcgcttcctg 1140

ctggagctgg gctgcgagcc gacggtgatc ctcagtcata acgccaataa acgctggcaa 1200

aaagcgatga agaaaatgct cgatgcctcg ccgtacggtc aggaaagcga agtgttcatc 1260

aactgcgacc tgtggcactt ccggtcgctg atgttcaccc gtcagccgga ctttatgatc 1320

ggtaactcct acggcaagtt tatccagcgc gataccctgg caaagggcaa agccttcgaa 1380

gtgccgctga tccgtctggg ctttccgctg ttcgaccgcc atcatctgca ccgccagacc 1440

acctggggct atgaaggcgc aatgaacatc gtcacgacgc tggtgaacgc cgtgctggaa 1500

1560

taa 1563

<210> 103

<211> 1485

<212> DNA

<213> Klebsiella variicola

<220>

<223> nifL

<400> 103

atgaccctga atatgatgct cgataacgcc gcgccgggagg ccatcgccgg cgcgctgact 60

caacaacatc cggggctgtt ttttaccatg gtggaacagg cctcggtggc catctccctc 120

accgatgcca gcgccaggat catttacgcc aacccggcgt tttgccgcca gaccggctat 180

tcgctggcgc aattgttaaa ccagaacccg cgcctgctgg ccagcagcca gacgccgcgc 240

gagatctatc aggagatgtg gcataccctg ctccagcgtc agccctggcg cggtcagctg 300

attaatcagc gtcgggacgg cggcctgtac ctggtggaga ttgacatcac cccggtgctt 360

agcccgcaag gggaactgga gcattatctg gcgatgcagc gggatatcag cgtcagctac 420

accctcgaac agcggctgcg caaccatatg accctgatgg aggcggtgct gaataatatc 480

cccgccgccg tggtagtggt ggacgagcag gatcgggtgg tgatggacaa cctcgcctac 540

aaaaccttct gcgctgactg cggcggccgg gagctgctca ccgagctgca ggtctcccct 600

ggccggatga cgcccggcgt ggaggcgatc ctgccggtgg cgctgcgcgg ggccgcgcgc 660

tggctgtcgg taacctgctg gccgttgccc ggcgtcagtg aagaggccag ccgctacttt 720

atcgacagcg cgctggcgcg gaccctggtg gtgatcgccg actgtaccca gcagcgtcag 780

cagcaggagc aagggcgcct tgaccggctg aagcagcaaa tgaccgccgg caagctgctg 840

gcggcgatcc gcgagtcgct ggacgccgcg ctgatccagc tgaactgccc gattaatatg 900

ctggcggcag cccgtcggct gaacggcgag ggaagcggga atgtggcgct ggaggccgcc 960

tggcgtgaag gggaagaggc gatggcgcgg ctccagcgct gtcgcccatc gctggaactc 1020

1080

acacgcttcg atcccgacgg gctgcaggtc gacatggcct caccgcatct gatcggcttt 1140

ggccagcgca ccccactgct ggcgtgctta agcctgtggc tcgatcgcac cctggccctc 1200

gccgccgaac tcccctccgt gccgctggcg atgcagctct acgccgagga gaacgacggc 1260

tggctgtcgc tgtatctgac tgacaacgta ccgctgctgc aggtgcgcta cgctcactcc 1320

cccgacgcgc tgaactcgcc gggcaaaggc atggagctgc ggctgatcca gaccctggtg 1380

gcgcaccatc gcggggccat tgagctggct tcccgaccgc agggcggcac ctgcctgacc 1440

ctgcgtttcc cgctgtttaa caccctgacc ggaggtgaag catga 1485

<210> 104

<211> 1575

<212> DNA

<213> Klebsiella variicola

<220>

<223> nifA

<400> 104

atgatccctg aatccgaccc ggacaccacc gtcagacgct tcgacctctc tcagcagttc 60

accgccatgc agcggataag cgtggtgctg agccggggcca ccgaggccag caaaacgctg 120

caggaggtgc tcagcgtatt acacaacgat gcctttatgc agcacgggat gatctgcctg 180

tacgacagcg agcaggagat cctcagtatc gaagcgctgc agcaaaccgg ccagcagccc 240

ctccccggca gcacgcagat ccgctatcgc cccggcgagg gactggtggg gaccgtgctg 300

gcccaggggc agtcgctggg gctgccccgg gtcgccgacg atcagcgttt tctcgaccgc 360

ctgagcctct acgattacga tctgccgttt atcgccgtac cgttgatggg gcccaacgcc 420

cggccaatag gggtgctggc ggcccagccg atggcgcgcc aggaagagcg gctgccggcc 480

tgcacccgtt ttctcgaaac cgtcgccaac ctcgtcgccc agaccatccg gctgatgatc 540

cttccggcct cacccgccct gtcgagccgc cagccgccga aggtggaacg gccgccggcc 600

tgctcgtcgt cgcgcggcgt gggccttgac aatatggtcg gcaagagccc ggcgatgcgc 660

cagatcgtgg aggtgatccg tcaggtttcg cgctgggaca ccaccgtgct ggtacgcggc 720

gaaagcggca ccgggaaaga gctgatcgcc aacgccatcc atcaccattc gccacgggct 780

ggcgccgcct tcgtcaaatt taactgcgcg gcgctgccgg acaccctgct ggaaagcgaa 840

ctgttcggcc atgagaaagg cgcctttacc ggggcggtgc gtcagcgtaa aggacgtttt 900

gagctggcgg atggcggcac cctgttcctc gatgagattg gtgaaagcag cgcctcgttc 960

caggccaagc tgctgcgtat cctccaggag ggggagatgg agcggggtcgg cggcgatgag 1020

accctgcggg tgaatgtccg catcatcgcc gccaccaacc gtcacctgga ggaggaggtc 1080

cggctgggcc atttccgcga ggatctctac tatcgtctga acgtgatgcc catcgccctg 1140

cccccgctgc gcgagcgtca ggaggacatc gccgagctgg cgcacttcct ggtgcgcaaa 1200

atcggccagc atcaggggcg cacgctgcgg atcagcgagg gcgcgatccg cctgctgatg 1260

gagtacagct ggccgggtaa cgttcgcgaa ctggagaact gcctcgaacg atcggcggtg 1320

atgtcggaga gtggcctgat cgatcgcgac gtgatcctct tcactcacca ggatcgtccc 1380

gccaaagccc tgcctgccag cgggccagcg gaagacagct ggctggacaa cagcctggac 1440

gaacgtcagc gactgatcgc cgcgctggaa aaagccggct gggtgcaggc caaggcggca 1500

cggctgctgg ggatgacgcc gcgccaggtc gcttatcggga tccagatcat ggatatcacc 1560

ctgccgcgtc tgtag 1575

<210> 105

<211> 2838

<212> DNA

<213> Klebsiella variicola

<220>

<223> glnE

<400> 105

atgatgccgc tttctccgca attacagcag cactggcaga cggtcgctga ccgtctgcca 60

gcggattttc ccattgccga actgagccca caggccaggt cggtcatggc gttcagcgat 120

tttgtcgaac agagtgtgat cgcccagccg ggctggctga atgagcttgc ggactcctcg 180

ccggaggcgg aagagtggcg gcattacgag gcctggctgc aggatcgcct gcaggccgtc 240

actgacgaag cggggttgat gcgagagctg cgtctcttcc gccgccagat gatggtccgc 300

atcgcctggg cgcaggcgct gtcgctggtg agcgaagaag agactctgca gcagctgagc 360

gtcctggcgg agaccctgat tgtcgccgcc cgcgactggc tgtacgccgc ctgctgtaag 420

gagtggggaa cgccatgcaa tgccgagggc cagccgcagc cgctgctgat cctcgggatg 480

ggaaagctgg gcggcggcga gctgaacttc tcttccgata tcgatctgat ctttgcctgg 540

cctgagcatg gcgccacccg cggcggccgc cgcgagctgg ataacgccca gttctttacc 600

cgtctggggc agcggctgat caaggccctt gaccagccga cgcaggacgg ctttgtctat 660

cgggttgaca tgcgcctgcg gccgtttggc gacagtgggc cgctggtact cagttttgcg 720

gcgctggaag attattacca ggagcagggt cgggactggg aacgctatgc gatggtgaaa 780

gcgcggatca tgggcgataa cgacggcgtg tacgccagcg agttgcgcgc gatgctccgt 840

cctttcgtct tccgccgtta tatcgacttc agcgtgatcc agtcgctgcg taacatgaaa 900

ggcatgatcg cccgcgaagt gcggcgtcgc gggctgaaag acaacatcaa gctcggcgcc 960

ggcgggatcc gtgaaattga gtttatcgtt caggtctttc aactgatccg cggtggtcgc 1020

gaacctgcac tgcagcagcg cgccctgctg ccgacgctgg cggcgattga tgagctacat 1080

ctgctgccgg aaggcgacgc ggcgctgctg cgcgaggcct atctgttcct gcgccggctg 1140

gaaaacctgc tgcaaagcat caacgatgag cagacccaga ccctgccgca ggatgaactt 1200

aaccgcgcca ggctggcgtg ggggatgcat accgaagact gggagacgct gagcgcgcag 1260

ctggcgagcc agatggccaa cgtgcggcga gtgtttaatg aactgatcgg cgatgatgag 1320

gatcagtccc cggatgagca actggccgag tactggcgcg agctgtggca ggatgcgctg 1380

gaagaagatg acgccagccc ggcgctggcg catttaaacg ataccgaccg ccgtagcgtg 1440

ctggcgctga ttgccgattt tcgtaaagag ctggatcggc gcaccatcgg cccgcgcggc 1500

cgccaggtgc tggatcagct gatgccgcat ctgctgagcg aaatctgctc gcgcgccgat 1560

gcgccgctgc ctctggcgcg gatcacgccg ctgttgaccg ggatcgtcac ccgtaccacc 1620

tatcttgagc tgctgagcga attccccggc gcgctgaagc acctgatcac gctctgcgcg 1680

gcgtcgccga tggtcgccag ccagctggcg cgccacccgc tgctgctgga tgagctgctg 1740

gatcccaaca ccctctatca gccgacggcg accgatgcct atcgcgacga gctgcgccag 1800

tacctgctgc gcgtgccgga agaggatgaa gagcagcagc tggaggcgtt gcgccagttt 1860

aagcaggcgc agcagctgca tatcgcggcg gcggatatcg ctggtaccct gccggtgatg 1920

aaggtcagcg atcacttaac ctggcttgcc gaagcgatcc tcgacgcggt ggtgcagcag 1980

gcatgggggc agatggtcgc tcgctacggc cagccgaccc acctgcacga tcgccagggt 2040

cgcggcttcg ccgtcgtcgg ctacggtaag cttggcggct gggagctggg ctacagctcc 2100

gatctcgatc tggtgttcct ccatgactgc ccggcggagg tgatgaccga cggcgagcgg 2160

gagattgacg gccgtcagtt ctacctgcgg ctggcccagc ggatcatgca cctgttcagc 2220

acccgcacct cgtccggtat tctctacgaa gtggacgccc ggctgcgtcc ttctggcgcg 2280

gcggggatgc tggtcaccac cgccgacgcg tttgctgact atcagcagaa cgaagcctgg 2340

acgtgggaac atcaggcgct ggtgcgcgcc cgcgtggtct atggcgaccc ggcgctgcag 2400

gcgcgctttg acgccattcg tcgcgatatc ctgaccaccc cgcgggaggg gatgaccctg 2460

cagaccgagg ttcgcgagat gcgcgagaag atgcgcgccc accttggcaa caaacatccc 2520

gatcgttttg atatcaaagc cgatgccggc gggatcaccg atattgaatt tattactcag 2580

2640

cgtattcttg agctgctggc gcagaacgac atcatggacg aggaggaggc gcgcgcctta 2700

acgcatgcgt acaccacctt gcgtgatgcg ctccatcacc tggccctgca ggagcagccg 2760

ggacacgtgg cgccagaggc cttcagccgg gagcgtcagc aggtcagcgc cagctggcag 2820

aagtggctga tggcttaa 2838

<210> 106

<211> 500

<212> DNA

<213> Klebsiella variicola

<220>

<223> PinfC

<400> 106

agcgtcaggt accggtcatg attcaccgtg cgattctcgg ttccctggag cgcttcattg 60

gcatcctgac cgaagagttc gctggcttct tcccaacctg gattgcacca gtgcaggtag 120

tggtcatgaa tattaccgat tctcaggctg aatacgttaa cgaattgacg cgtaaactac 180

aaaatgcggg cattcgtgta aaagcagact tgagaaatga gaagattggc tttaaaatcc 240

gcgagcacac tttacgtcgt gtcccgtata tgttggtctg tggcgacaaa gaagtcgaag 300

ccggcaaagt ggccgtgcgc acccgtcgcg ggaaagacct cggcagcatg gacgtaagtg 360

aagtgattga gaagctgcaa caagagattc gcagccgcag tcttcaacaa ctggaggaat 420

aaggtattaa aggcggaaaa cgagttcaaa cggcacgtcc gaatcgtatc aatggcgaga 480

ggaagttcgc 500

<210> 107

<211> 1287

<212> DNA

<213> Klebsiella variicola

<220>

<223> amtB

<400> 107

atgaaaatgg caacaatgaa atcgggtctg ggggcattag cccttcttcc gggactggca 60

atggccgcgc ccgcagtggc ggacaaagcc gataacgcgt ttatgatgat ttgcaccgcg 120

ctggttctgt ttatgaccat cccggggatc gcgctgtttt acggcggcct gatccgcggc 180

aaaaacgtcc tttccatgct gactcaggtg attgtgacct ttggcctggt ctgcgtactg 240

tgggtgattt atggctatac cctggccttc ggcaccggcg gcagcttctt cggtagtttt 300

gactgggtga tgctgaaaaa tattgaactg aaagcgctga tgggcacctt ctatcagtac 360

atccacgtgg ccttccaggg ctcgttcgcc tgtatcaccg tcgggctgat cgtgggggcg 420

ctggctgagc gtattcgttt ctccgccgtg ctgatttttg tggtggtgtg gatgacgctc 480

tcttatgttc cgattgcgca catggtctgg ggcggcggtc tgctggcgac ccacggcgcg 540

ctggacttcg cgggcggcac cgttgtacac atcaacgccg cggttgccgg gctggtgggt 600

gcgtacatga tgggcaaacg tgtgggcttc ggcaaagaag cgttcaaacc gcacaatctg 660

ccgatggtgt tcaccggaac cgccatcctc tacgtgggct ggttcggctt caacgccggc 720

tccgccagcg cagcgaacga aattgccgca ttggctttcg tcaacaccgt cgtcgccaca 780

gcggctgcca tcctggcgtg gaccttttggc gaatgggccc tgcgcggtaa accttcactg 840

ctgggcgcct gctccggggc gattgccggt ctggttggcg tcacaccagc ctgtgggtat 900

atcggtgtcg gtggggcgtt gattgtgggt atcgcatctg gtctggcggg catctggggc 960

gtaacggcgc tgaaacgctg gctgcgggtt gatgaccctt gcgacgtctt cggcgtccac 1020

ggcgtctgcg gcatcgtcgg ctgtatcctg accggtatct tcgcggccac ctctctgggc 1080

ggcgtgggtt atgcagaagg cgtcaccatg ggccatcagc tgctggtgca actcgagagt 1140

atcgcgatta ccatcgtctg gtcgggcgtt gtcgctttca ttggctacaa agtggcggac 1200

atgaccgtgg ggctgcgcgt accagaagag caggagcgcg aaggactgga cgtcaacagc 1260

catggcgaaa acgcctacaa cgcctga 1287

<210> 108

<211> 299

<212> DNA

<213> Klebsiella variicola

<220>

<223> Prm8.2

<400> 108

cgccgtcctc gcagtaccat tgcaaccgac tttacagcaa gaagtgattc tggcacgcat 60

ggaacaaatt cttgccagtc gggctttatc cgatgacgaa cgcgcacagc ttttatatga 120

gcgcggagtg ttgtatgata gtctcggtct gagggcatta gcgcgaaatg atttttcaca 180

agcgctggca atccgacccg atatgcctga agtattcaat tacttaggca tttacttaac 240

gcaggcaggc aattttgatg ctgcctatga agcgtttgat tctgtacttg agcttgatc 299

<210> 109

<211> 300

<212> DNA

<213> Klebsiella variicola

<220>

<223> Prm6.2

<400> 109

gctaaagttc tcggctaatc gctgataaca tttgacgcaa tgcgcaataa aagggcatca 60

tttgatgccc tttttgcacg ctttcatacc agaacctggc tcatcagtga ttttttttgt 120

cataatcatt gctgagacag gctctgaaga gggcgtttat acaccaaacc attcgagcgg 180

tagcgcgacg gcaagtcagc gttctccttt gcaatagcag ggaagaggcg ccagaaccgc 240

cagcgttgaa gcagtttgaa cgcgttcagt gtataatccg aaacttaatt tcggtttgga 300

<210> 110

<211> 400

<212> DNA

<213> Klebsiella variicola

<220>

<223> Prm1.2

<400> 110

gcccgctgac cgaccagaac ttccaccttg gactcggcta tacccttggc gtgacggcgc 60

gcgataactg ggactacatc cccattccgg tgatcttacc attggcgtca ataggttacg 120

gtccggcgac tttccagatg acctatattc ccggcaccta caataacggt aacgtttact 180

tcgcctgggc tcgtatacag ttttaattcg ctaagtctta gcaataaatg agataagcgg 240

tgtgtcttgt ggaaaaacaa ggactaaagc gttacccact aaaaaagata gcgactttta 300

tcacttttta gcaaagttgc actggacaaa aggtaccaca attggtgtac tgatactcga 360

cacagcatta gtgtcgattt ttcatataaa ggtaattttg 400

<210> 111

<211> 1536

<212> DNA

<213> Klebsiella variicola

<220>

<223> 16S

<220>

<221> modified base

<222> (245)..(245)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (452)..(452)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (454)..(454)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (1131)..(1132)

<223> a, c, t, g, unknown or other

<400> 111

ttgaagagtt tgatcatggc tcagattgaa cgctggcggc aggcctaaca catgcaagtc 60

gagcggtagc acagagagct tgctctcggg tgacgagcgg cggacgggtg agtaatgtct 120

gggaaactgc ctgatggagg gggataacta ctggaaacgg tagctaatac cgcataacgt 180

cgcaagacca aagtggggga ccttcgggcc tcatgccatc agatgtgccc agatgggatt 240

agctngtagg tggggtaacg gctcacctag gcgacgatcc ctagctggtc tgagaggatg 300

accagccaca ctggaactga gacacggtcc agactcctac gggaggcagc agtggggaat 360

attgcacaat gggcgcaagc ctgatgcagc catgccgcgt gtgtgaagaa ggccttcggg 420

ttgtaaagca ctttcagcgg ggaggaaggc gntnaggtta ataaccttgt cgattgacgt 480

tacccgcaga agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc 540

aagcgttaat cggaattact gggcgtaaag cgcacgcagg cggtctgtca agtcggatgt 600

gaaatccccg ggctcaacct gggaactgca ttcgaaactg gcaggctaga gtcttgtaga 660

ggggggtaga attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg 720

cgaaggcggc cccctggaca aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag 780

gattagatac cctggtagtc cacgctgtaa acgatgtcga tttggaggtt gtgcccttga 840

ggcgtggctt ccggagctaa cgcgttaaat cgaccgcctg gggagtacgg ccgcaaggtt 900

aaaactcaaa tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat 960

gcaacgcgaa gaaccttacc tggtcttgac atccacagaa ctttccagag atggattggt 1020

gccttcggga actgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt 1080

1140

actcaaagga gactgccagt gataaactgg aggaaggtgg ggatgacgtc aagtcatcat 1200

ggcccttacg accagggcta cacacgtgct acaatggcat atacaaagag aagcgacctc 1260

gcgagagcaa gcggacctca taaagtatgt cgtagtccgg attggagtct gcaactcgac 1320

tccatgaagt cggaatcgct agtaatcgta gatcagaatg ctacggtgaa tacgttcccg 1380

ggccttgtac acaccgcccg tcacaccatg ggagtgggtt gcaaaagaag taggtagctt 1440

aaccttcggg agggcgctta ccactttgtg attcatgact ggggtgaagt cgtaacaagg 1500

taaccgtagg ggaacctgcg gttggatcac ctcctt 1536

<210> 112

<211> 882

<212> DNA

<213> Klebsiella variicola

<220>

<223> nifH

<400> 112

atgaccatgc gtcaatgcgc tatctacggt aaaggcggta tcggtaaatc caccaccacc 60

cagaatctcg tcgcggccct cgccgagatg ggtaagaaag tgatgatcgt cggctgcgat 120

ccgaaagcgg actccaccg tctgatcctt cacgctaaag cccagaacac catcatggag 180

atggcggcgg aagtgggctc ggtcgaggat ctggagctcg aagacgttct gcaaatcggc 240

tatggcgatg tccgttgcgc cgaatccggc ggcccggagc caggcgtcgg ctgcgccgga 300

cgcggggtga tcaccgccat caacttcctc gaggaagaag gcgcctatga ggaagatttg 360

gatttcgtct tctatgacgt cctcggcgac gtagtctgcg gcggcttcgc catgccgatc 420

cgcgaaaaca aagccgga gatctacatc gtctgctccg gcgagatgat ggcgatgtat 480

gccgccaaca atatctccaa ggggatcgtg aagtacgcga aatctggcaa ggtgcgcctc 540

ggcggcctga tctgtaactc gcgcaaaacc gaccgggaag acgaactgat catcgccctg 600

gcggagaagc ttggcacgca gatgatccac ttcgttcccc gcgacaacat tgtgcagcgc 660

gcggagatcc gccggatgac ggtgatcgag tacgacccga cctgtcagca ggcgaatgaa 720

tatcgtcaac tggcgcagaa gatcgtcaat aacaccaaaa aagtggtgcc aacgccgtgc 780

accatggacg agctggaatc gctgctgatg gagttcggca tcatggaaga agaagacacc 840

agcatcattg gtaaaaccgc cgctgaagaa aacgcggcct ga 882

<210> 113

<211> 1449

<212> DNA

<213> Klebsiella variicola

<220>

<223> nifD1

<400> 113

atgaccaacg caacaggcga acgtaacctt gcgctcatcc aggaagtcct ggaggtgttt 60

cccgaaaccg cgcgcaaaga gcgcagaaag cacatgatga tcagcgatcc gcagatggag 120

agcgtcggca agtgcattat ctcgaaccgt aaatcgcagc ccggggtgat gaccgtgcgt 180

ggctgcgcct atgcgggctc gaaaaggggtg gtgtttgggc caatcaaaga catggcccat 240

atctcgcacg gccccatcgg ctgcggccag tactcgcgcg ccggacggcg caactactat 300

accggcgtca gcggtgtcga cagcttcggc accctgaact tcacctctga ttttcaggag 360

cgcgatattg ttttcggcgg cgataaaaag ctgaccaaac tgatcgaaga gatggagctg 420

ctgttcccgc tgaccaaagg gatcaccatc cagtcggagt gcccggtggg cctgatcggc 480

gatgacatca gcgccgtggc caacgccagc agcaaggcgc tggataaacc ggtgatcccg 540

gtgcgctgcg aaggctttcg cggcgtatcg caatcgctgg gccaccatat cgccaacgac 600

gtggtgcgcg actgggtgct gaacaatcgc gaagggcagc cgtttgccag caccccgtat 660

gatgttgcca tcattggcga ttacaacatc ggcggcgacg cctgggcctc gcgcattctg 720

ctggaagaga tggggctgcg cgtagtggcg cagtggtccg gcgacggcac cctggtggag 780

atggagaaca ccccattcgt taagcttaac ctcgtccact gctaccgttc gatgaactat 840

atcgcccgcc atatggagga gaaacatcag atcccgtgga tggaatataa cttcttcggc 900

ccgaccaaaa tcgccgaatc gctgcgcaag atcgccgatc aatttgatga caccattcgc 960

gccaatgcgg aagcggtgat cgccaaatat gaggggcaga tggcggccat catcgccaaa 1020

tatcgcccgc ggctggaggg gcgcaaagtg ctgctgtaca tgggggggct gcggccgcgc 1080

cacgtcatcg gcgcctatga ggatctcggg atggagatca tcgccgccgg ctacgagttt 1140

gcccataacg atgattacga ccgcaccctg ccggacctga aagagggcac cctgctgttt 1200

gacgatgcca gcagctatga gctggaggcc ttcgtcaaag cgctgaaacc tgacctcatc 1260

ggctccggga tcaaagagaa atatatcttc cagaaaatgg gggtgccgtt ccgccagatg 1320

cactcctggg actattccgg cccctatcac ggctatgacg gcttcgccat ctttgcccgc 1380

gatatggata tgaccctgaa caatccggcg tggaacgaac tgactgcccc gtggctgaag 1440

tctgcgtga 1449

<210> 114

<211> 1374

<212> DNA

<213> Klebsiella variicola

<220>

<223> nifD2

<400> 114

atgaagggaa aggaaattct ggcgctgctg gacgaacccg cctgcgagca caaccagaag 60

caaaaatccg gctgcagcgc tcctaagccc ggcgcaaccg ccggcggctg cgccttcgac 120

ggcgcgcaga taacgctcct gcccatcgcc gacgtcgcgc acctggtgca cggccccatc 180

ggctgcgcgg gcagctcgtg ggataaccgc ggcagcgtca gcgccggccc ggccctcaac 240

cggctcggct ttaccaccga tcttaacgaa caggatgtga ttatggggccg cggcgaacgc 300

cgcctgttcc acgccgtccg tcacatcgtc gaccgctatc atccggcggc ggtctttatc 360

tacaacacct gcgtaccggc gatggagggg gatgacctgg aggccgtctg ccaggccgca 420

cagaccgcca ccggcgtccc ggtcatcgcc attgacgccg ccggtttcta cggcagtaaa 480

aatcttggca accgaatggc gggcgacgtg atgctcaggc aggtgattgg ccagcgcgaa 540

ccggccccgt ggccagacaa cacgcccttt gccccggccc agcgccacga tatcggcctg 600

attggcgaat tcaatatcgc cggcgagttc tggcaggtcc agccgctgct cgacgagctg 660

gggatccgcg tcctcggcag cctctccggc gacggccgct ttgccgagat ccagaccctg 720

caccgggcgc aggccaatat gctggtgtgc tcgcgcgcgc tgatcaacgt cgcccggggg 780

ctggagctgc gctacggcac gccgtggttt gaaggcagct tctacgggat ccgcgccacc 840

tccgacgcct tgcgccagct ggcggcgctg ctgggggatg acgacctgtg ccgccgcacc 900

gaggcgctga tcgcccgcga agagcaggcg gcggagcagg cgctggcgcc gtggcgcgag 960

cagctccgtg ggcgcaaagt gttgctctac accggcggcg tgaaatcctg gtcggtggta 1020

tcagccctgc aggatctcgg catgaccgtg gtggccaccg gcacgcggaa atccaccgag 1080

gaggacaaac agcggatccg tgagctgatg ggcgacgagg cggtgatgct tgaggagggc 1140

aatgcccgca ccctgctcga cgtggtgtac cgctatcagg ccgacctgat gatcgccggc 1200

ggacgcaata tgtacaccgc ctggaaagcc cggctgccgt ttctcgatat caatcaggag 1260

cgcgagcacg cctacgccgg ctatcagggc atcatcaccc tcgcccgcca gctctgtctg 1320

accctcgcca gtcccgtctg gccgcaaacg catacccgcg ccccgtggcg ctag 1374

<210> 115

<211> 1386

<212> DNA

<213> Klebsiella variicola

<220>

<223> nifK1

<400> 115

atggcagaca ttatccgcag tgaaaaaccg ctggcggtga gcccgattaa aaccgggcaa 60

ccgctcgggg cgatcctcgc cagcctcggg ctggcccagg ccatcccgct ggtcccggc 120

gcccagggct gcagcgcctt cgccaaagtt ttcttttattc agcatttcca tgacccggtg 180

ccgctgcagt cgacggccat ggatccgacc gccacgatca tgggggccga cggcaatatc 240

ttcaccgcgc tcgacaccct ctgccagcgc cacagcccgc aggccatcgt gctgctcagc 300

accggtctgg cggaagcgca gggcagcgat atcgcccggg tggtgcgcca gtttcgtgag 360

gcgcatccgc gccataacgg cgtggcgatc ctcaccgtca ataccccggga tttttttggc 420

tcgatggaaa acggctacag cgcggtgatc gagagcgtga tcgagcagtg ggtcgcgccg 480

acgccgcgtc cggggcagcg gccccggcgg gtcaacctgc tggtcagcca cctctgttcg 540

ccaggggata tcgaatggct gggccgctgc gtggaggcct ttggcctgca gccggtgatc 600

ctgccggacc tctcgcagtc aatggatggc cacctcggtg aaggggattt tacgcccctg 660

acccagggcg gcgcctcgct gcgccagatt gcccagatgg gccagagtct gggcagcttc 720

gccattggcg tgtcgctcca gcgggcggca tcgctcctga cccaacgcag ccgcggcgac 780

gtgatcgccc tgccgcatct gatgaccctc gaccattgcg atacctttat ccatcagctg 840

gcgaagatgt ccggacgccg cgtaccggcc tggattgagc gccagcgcgg ccagctgcag 900

gatgcgatga tcgactgcca tatgtggctt cagggccagc gcatggcgat ggcggcggag 960

ggcgacctgc tggcggcgtg gtgtgatttc gcccgcagcc aggggatgca gcccggcccg 1020

ctggtcgccc ccaccagcca ccccagcctg cgccagctgc cggtcgatca ggtcgtgccg 1080

ggggatcttg aggatctgca gcagctgctg agccaccaac ccgccgatct gctggtggct 1140

aactctcacg cccgcgatct ggcggagcag tttgccctgc cgctgatccg cgtcggtttt 1200

cccctcttcg accggctcgg tgagtttcgt cgcgtccgcc aggggtacgc cggtatgcga 1260

gatacgctgt ttgagctggc caatctgctg cgcgaccgcc atcaccacac cgccctctac 1320

1380

cattaa 1386

<210> 116

<211> 183

<212> DNA

<213> Klebsiella variicola

<220>

<223> nifK2

<400> 116

gtgccgctga tccgtctggg ctttccgctg ttcgaccgcc atcatctgca ccgccagacc 60

acctggggct atgaaggcgc aatgaacatc gtcacgacgc tggtgaacgc cgtgctggaa 120

180

taa 183

<210> 117

<211> 1485

<212> DNA

<213> Klebsiella variicola

<220>

<223> nifL

<400> 117

atgaccctga atatgatgct cgataacgcc gcaccgggagg ccatcgccgg cgcgctgact 60

caacaacatc cggggctgtt ttttaccatg gtggaacagg cctcggtggc catatccctc 120

accgatgcca gcgccaggat catttacgcc aacccagcgt tttgccgcca gaccggctat 180

tcgctggcgc aattgttaaa ccagaacccg cgcctgctgg ccagcagcca gacgccgcgc 240

gcgatctatc aggagatgtg gcataccctg ctccagcgtc agccctggcg cggtcagctg 300

attaatcagc gtcgggacgg cggcctgtgc ctggtggaga ttgacatcac cccggtgctt 360

agcccgcaag gggaactgga gcattatctg gcgatgcagc gggatatcag cgtcagctac 420

accctcgaac aacggctgcg caaccatatg accctgatgg aggcggtgct gaataatatc 480

cccgccgccg tggtggtggt ggacgagcag gatcgggtgg tgatggacaa cctcgcctac 540

aaaaccttct gcgctgactg cggcggccgg gagctgctca ccgagctgca ggtctcccct 600

ggccggatga cgcccggcgt ggaggcgatc ctgccggtag cgctgcgcgg ggccgcgcgc 660

tggctgtcgg taacctgctg gccgttgccc ggcgtcagtg aagaggccag ccgctacttt 720

atcgacagcg cgctggcgcg gaccctggtg gtgatcgccg actgtaccca gcagcgtcag 780

cagcaggagc aaggacgcct tgaccggctg aagcagcaaa tgaccgccgg caagctgctg 840

gcggcgatcc gcgagtcgct ggacgccgcg ctgatccagc tgaactgccc gattaatatg 900

ctggcggcag cccgtcggct gaacggcgag ggaagcggga atgtggcgct ggaggccgcc 960

tggcgtgaag gggaagaggc gatggcgcgg ctccagcgct gtcgcccatc gctggaactc 1020

1080

acccgcttcg atcccgacgg gctgcaggtc gacatggcct caccgcatct gatcggcttt 1140

ggccagcgca ccccgctgct ggcgtgctta agcctgtggc tcgaccgcac cctggccctc 1200

gccgccgaat tgccctccgt gccgctggcg atgcagctct atgccgagga gaacgacggc 1260

tggctgtcgc tgtacctgac tgataacgta ccgctgttgc aggtgcgcta cgcccactcc 1320

cccgacgcgc tgaactcgcc gggtaaaggc atggagctgc ggctgatcca gaccctggtg 1380

gcgcaccatc gcggggccat tgagctggct tcccgaccgc agggcggcac ctgcctgacc 1440

ctgcgtttcc cgctgtttaa caccctgacc ggaggtgaag catga 1485

<210> 118

<211> 1575

<212> DNA

<213> Klebsiella variicola

<220>

<223> nifA

<400> 118

atgatccctg aatccgaccc ggacaccacc gtcagacgct tcgacctctc tcagcagttc 60

accgccatgc agcggataag cgtggtgctg agccggggcca ccgaggccag caaaacgctg 120

caggaggtac tcactgtatt gcacaacgat gcctttatgc agcacgggat gatctgcctg 180

tacgacagcg agcaggagat cctcagtatc gaagcgctgc agcaaaccgg ccagcagccc 240

ctccccggca gcacgcagat ccgctatcgc cccggcgagg gactggtggg gaccgtgctg 300

gcccaggggc agtcgctggg gctgccccgg gtcgccgacg atcagcgttt tctcgaccgc 360

ctgagcctct acgattacga tctgccgttt atcgccgtac cgttgatggg gcccaacgcc 420

cggccaatag gggtgctggc ggcccagccg atggcgcgcc aggaagagcg gctgccggcc 480

tgcacccgtt ttctcgaaac cgtcgccaac ctcgtcgccc agaccatccg gctgatgatc 540

cttccggcct cacccgccct gtcgagccgc cagccgccga aggtggaacg gccgccggcc 600

tgctcgtcgt cgcgcggcgt gggccttgac aatatggtcg gcaagagccc ggcgatgcgc 660

cagatcgtgg aggtgatccg tcaggtttcg cgctgggaca ccaccgtgct ggtgcgcggt 720

gaaagcggca ccgggaaaga gctgatcgcc aacgccatcc atcaccattc gccacgggct 780

ggcgccgcct tcgtcaaatt taactgcgcg gcgctgccgg acaccctgct ggaaagcgaa 840

ctgttcggcc atgagaaagg cgcctttacc ggggcggtgc gtcagcgtaa aggacgtttt 900

gagctggcgg atggcggcac cctgttcctc gatgagattg gtgaaagcag cgcctcgttc 960

caggccaagc tgctgcgtat cctccaggag ggggagatgg agcggggtcgg cggcgatgag 1020

accctgcggg tgaatgtccg catcatcgcc gccaccaacc gtcacctgga ggaggaggtc 1080

cggctgggcc atttccgcga ggatctctat tatcgtctga acgtgatgcc catcgccctg 1140

cccccgctgc gcgagcgtca ggaggacatc gccgagctgg cgcacttcct ggtgcgcaaa 1200

atcggccagc atcaggggcg cacgctgcgg atcagcgagg gcgcgatccg cctgctgatg 1260

gagtacagct ggccgggtaa cgttcgcgaa ctggagaact gcctcgaacg atcggcggtg 1320

atgtcggaga gtggcctgat cgatcgcgac gtgatcctct tcactcacca ggatcgtccc 1380

gccaaagccc tgcctgccag cgggccagcg gaagacagct ggctggacaa cagcctggac 1440

gaacgtcagc gactgatcgc cgcgctggaa aaagccggct gggtgcaggc caaggcggca 1500

cggctgctgg ggatgacgcc gcgccaggtc gcttaccggga tccagatcat ggatatcacc 1560

ctgccgcgtc tgtag 1575

<210> 119

<211> 2838

<212> DNA

<213> Klebsiella variicola

<220>

<223> glnE

<400> 119

atgatgccgc tttctccgca attacagcag cactggcaga cggtcgctga ccgtctgcca 60

gcggattttc ccattgcaga actgagccca caggccaggt cggtcatggc gttcagcgat 120

tttgtcgaac agagtgtgat cgcccagccg ggctggctga atgagcttgc ggactcctcg 180

ccggaggcgg aagagtggcg gcattacgag gcctggctgc aggatcgcct gcaggccgtc 240

actgacgaag cggggttgat gcgagagctg cgtctcttcc gccgccagat gatggtccgc 300

atcgcctggg cgcaggcgct gtcgctggtg agcgaagaag agaccctgca gcagctgagc 360

gccctggcgg agaccctgat tgtcgccgcc cgcgactggc tctacgccgc ctgctgtaag 420

gagtggggaa cgccatgcaa tgccgagggc cagccgcagc cgctgctgat cctcgggatg 480

ggaaagctgg gcggcggcga gctgaacttc tcttccgata tcgatctgat ctttgcctgg 540

cctgagcatg gcgccacccg cggcggccgc cgcgagctgg ataacgccca gttctttacc 600

cgtctggggc agcggctgat caaggccctt gaccagccga cgcaggacgg ctttgtctat 660

cgggttgaca tgcgcctgcg gccgtttggc gacagtgggc cgctggtact cagctttgcg 720

gcactggaag attattacca ggagcagggt cgggactgggg aacgctatgc gatggtgaaa 780

gcgcggatca tgggcgataa cgacggcgtg tacgccagcg agttgcgcgc gatgctccgt 840

cctttcgtct tccgccgtta tatcgacttc agcgtgatcc agtcgctgcg taacatgaaa 900

ggcatgatcg cccgcgaagt gcggcgtcgc gggctgaaag acaacatcaa gctcggcgcc 960

ggcgggatcc gtgaaattga gtttatcgtt caggtctttc agctgatccg cggtggtcgc 1020

gaacctgcac tgcagcagcg cgccctgctg ccgacgctgg cggcgattga tgagctacat 1080

ctgctgccgg aaggcgacgc ggcgctgctg cgcgaggcct atctgttcct gcgccggctg 1140

gaaaacctgc tgcaaagcat caacgatgaa cagacccaga ccctgccgca ggatgaactt 1200

aaccgcgcca ggctggcgtg ggggatgcat accgaagact gggagacgct gagcgcgcag 1260

ctggcgagcc agatggccaa cgtgcggcga gtgtttaatg aactgatcgg cgatgatgag 1320

gatcagtccc cggatgagca actggccgag tactggcgcg agctgtggca ggatgcgctg 1380

gaagaagatg acgccagccc ggcgctggcg catttaaacg ataccgaccg ccgtagcgtg 1440

ctggcgctga ttgccgattt tcgtaaagag ctggatcggc gcaccatcgg cccgcgcggc 1500

cgccaggtgc tggatcagct gatgccgcat ctgctgagcg aaatctgctc gcgtgccgat 1560

gcgccgctgc ctctggcgcg gatcacgccg ctgttgaccg ggatcgtcac ccgtaccacc 1620

tatcttgagc tgctgagcga attccccggc gcgctgaagc acctgatcac gctctgcgcg 1680

gcgtcgccga tggtcgccag ccagctggcg cgccacccgc tgctgctgga tgagctgctg 1740

gatcccaaca ccctctatca gccgacggcg accgatgcct atcgcgacga gctgcgccag 1800

tacctgctgc gcgtgccgga agaggatgaa gagcagcagc tggaggcgtt gcgccagttt 1860

aagcaggcgc agcagctgca tatcgcggcg gcggatatcg ctggtaccct gccggtgatg 1920

aaggtcagcg atcacttaac ctggcttgcc gaagcgatcc tcgacgcggt ggtgcagcag 1980

gcatgggggc agatggtcgc tcgctacggt cagccgaccc acctgcacga tcgccagggt 2040

cgcggcttcg ccgttgtcgg ctacggtaag ctcggcggct gggagctggg ctacagctcc 2100

gatctcgatc tggtgttcct ccatgactgc ccggcggagg tgatgaccga cggcgagcgg 2160

gagattgacg gccgtcagtt ctacctgcgg ctggcccagc ggatcatgca cctgttcagc 2220

acccgcacct cgtccggtat tctctacgaa gtggacgccc ggctgcgtcc ttctggcgcg 2280

gcggggatgc tggtcaccac cgccgacgcg tttgctgact atcagcagaa cgaagcctgg 2340

acgtgggaac atcaggcgct ggtgcgcgcc cgcgtggtct atggcgaccc ggcgctgcag 2400

2460 gcgcgctttg acgccattcg tcgcgatatc ctgaccaccc

cagaccgagg ttcgcgagat gcgcgagaag atgcgcgccc accttggcaa caaacatccc 2520

gatcgttttg atatcaaagc cgatgccggc gggatcaccg atattgaatt tattactcag 2580

2640

cgtattcttg agctgctggc gcagaacgac atcatggacg aggaggaggc gcgcgcctta 2700

acgcatgcat acaccacctt gcgtgatgcg ctccatcacc tggccctgca ggagcagccg 2760

ggacacgtgg cgccagaggc cttcagccgg gagcgtcagc aggtcagcgc cagctggcag 2820

aagtggctga tggcttaa 2838

<210> 120

<211> 1287

<212> DNA

<213> Klebsiella variicola

<220>

<223> amtB

<400> 120

atgaaaatgg caacaatgaa atcgggtctg ggggcattag cccttcttcc gggactggca 60

atggccgcgc ccgcagtggc ggacaaagcc gataacgcgt ttatgatgat ttgcaccgcg 120

ctggttctgt ttatgaccat cccggggatc gcgctgtttt acggcggcct gatccgcggc 180

aaaaacgtcc tttccatgct gactcaggtg attgtgacct ttggcctggt ctgcgtactg 240

tgggtgattt atggctatac cctggccttc ggcaccggcg gcagcttctt cggtagcttt 300

gactgggtga tgctgaaaaa tattgaactg aaagcgctga tgggcacctt ctatcagtac 360

atccacgtgg ccttccaggg ctcgttcgcc tgtatcaccg tcgggctgat cgtgggggcg 420

ctggctgagc gtattcgttt ctccgccgtg ctgattttcg tggtggtgtg gatgacgctc 480

tcttatgttc cgattgcgca catggtctgg ggcggcggtc tgctggcgac ccacggcgcg 540

ctggacttcg cgggcggcac cgttgtacac atcaacgccg cggttgccgg gctggtgggt 600

gcgtatatga tgggcaaacg tgtgggcttc ggcaaagaag cgttcaaacc gcacaatctg 660

ccgatggtgt tcaccggaac cgccatcctc tacgtgggct ggttcggctt caacgccggc 720

tccgccagcg cagcgaacga aattgccgca ctggctttcg tcaacaccgt cgtcgccaca 780

gcggcagcca tcctggcctg gacctttggc gaatgggctc tgcgcggcaa accttcactg 840

ctgggcgcct gctccggggc gattgccggt ctggttggcg tcacaccagc ctgtgggtat 900

atcggtgtcg gtggggcgtt gattgtgggt atcgcatctg gtctggcggg catctggggc 960

gtaacggcgc tgaaacgctg gctgcgggtt gatgaccctt gcgacgtctt cggcgtccac 1020

ggcgtctgcg gcatcgtcgg ctgtatcctg accggtatct tcgcggccac ctctctgggc 1080

ggcgtgggtt atgcagaagg cgtcaccatg ggccatcagc tgctggtgca actcgagagt 1140

atcgcgatta ccatcgtctg gtcgggcgtt gtcgctttca ttggctacaa agtggcggac 1200

atgaccgtgg ggctgcgcgt accagaagag caggagcgcg aaggactgga cgtcaacagc 1260

catggcgaaa acgcctacaa cgcctga 1287

<210> 121

<211> 1435

<212> DNA

<213> Achromobacter spiritinus

<220>

<223> 16S

<220>

<221> modified base

<222> (255)..(255)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (454)..(454)

<223> a, c, t, g, unknown or other

<400> 121

ctgaagagtt tgatcctggc tcagattgaa cgctagcggg atgccttaca catgcaagtc 60

gaacggcagc acggacttcg gtctggtggc gagtggcgaa cgggtgagta atgtatcgga 120

180

gggggaaagc aggggatcgc aagaccttgc actattagag cggccgatat cggattagct 240

agttggtggg gtaanggctc accaaggcga cgatccgtag ctggtttgag aggacgacca 300

gccacactgg gactgagaca cggcccagac tcctacggga ggcagcagtg gggaattttg 360

gacaatgggg gaaaccctga tccagccatc ccgcgtgtgc gatgaaggcc ttcgggttgt 420

aaagcacttt tggcaggaaa gaaacgtcat gggntaatac cccgtgaaac tgacggtacc 480

tgcagaataa gcaccggcta actacgtgcc agcagccgcg gtaatacgta gggtgcaagc 540

gttaatcgga attactgggc gtaaagcgtg cgcaggcggt tcggaaagaa agatgtgaaa 600

tcccagagct taactttgga actgcatttt taactaccgg gctagagtgt gtcagaggga 660

ggtggaattc cgcgtgtagc agtgaaatgc gtagatatgc ggaggaacac cgatggcgaa 720

ggcagcctcc tgggataaca ctgacgctca tgcacgaaag cgtggggagc aaacaggatt 780

agataccctg gtagtccacg ccctaaacga tgtcaactag ctgttggggc cttcgggcct 840

tagtagcgca gctaacgcgt gaagttgacc gcctggggag tacggtcgca agattaaaac 900

tcaaaggaat tgacggggac ccgcacaagc ggtggatgat gtggattaat tcgatgcaac 960

gcgaaaaacc ttacctaccc ttgacatgtc tggaattctg aagagattcg gaagtgctcg 1020

caagagaacc ggaacacagg tgctgcatgg ctgtcgtcag ctcgtgtcgt gagatgttgg 1080

gttaagtccc gcaacgagcg caacccttgt cattagttgc tacgaaaggg cactctaatg 1140

agactgccgg tgacaaaccg gaggaaggtg gggatgacgt caagtcctca tggcccttat 1200

gggtagggct tcacacgtca tacaatggtc gggacagagg gtcgccaacc cgcgagggggg 1260

agccaatccc agaaacccga tcgtagtccg gatcgcagtc tgcaactcga ctgcgtgaag 1320

tcggaatcgc tagtaatcgc ggatcagcat gtcgcggtga atacgttccc gggtcttgta 1380

cacaccgcc gtcacaccat gggagtgggt tttaccagaa gtagttagcc taacc 1435

<210> 122

<211> 1528

<212> DNA

<213> Achromobacter marplatensis

<220>

<223> 16S

<220>

<221> modified base

<222> (255)..(255)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (999)..(999)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (1009)..(1009)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (1437)..(1437)

<223> a, c, t, g, unknown or other

<400> 122

ctgaagagtt tgatcctggc tcagattgaa cgctagcggg atgccttaca catgcaagtc 60

gaacggcagc acggacttcg gtctggtggc gagtggcgaa cgggtgagta atgtatcgga 120

180

gggggaaagc aggggatcgc aagaccttgc actattagag cggccgatat cggattagct 240

agttggtggg gtaanggctc accaaggcga cgatccgtag ctggtttgag aggacgacca 300

gccacactgg gactgagaca cggcccagac tcctacggga ggcagcagtg gggaattttg 360

gacaatgggg gaaaccctga tccagccatc ccgcgtgtgc gatgaaggcc ttcgggttgt 420

aaagcacttt tggcaggaaa gaaacgtcat gggttaatac cccgtgaaac tgacggtacc 480

tgcagaataa gcaccggcta actacgtgcc agcagccgcg gtaatacgta gggtgcaagc 540

gttaatcgga attactgggc gtaaagcgtg cgcaggcggt tcggaaagaa agatgtgaaa 600

tcccagagct taactttgga actgcatttt taactaccgg gctagagtgt gtcagaggga 660

ggtggaattc cgcgtgtagc agtgaaatgc gtagatatgc ggaggaacac cgatggcgaa 720

ggcagcctcc tgggataaca ctgacgctca tgcacgaaag cgtggggagc aaacaggatt 780

agataccctg gtagtccacg ccctaaacga tgtcaactag ctgttggggc cttcgggcct 840

tagtagcgca gctaacgcgt gaagttgacc gcctggggag tacggtcgca agattaaaac 900

tcaaaggaat tgacggggac ccgcacaagc ggtggatgat gtggattaat tcgatgcaac 960

gcgaaaaacc ttacctaccc ttgacatgtc tggaattcng aagagattng gaagtgctcg 1020

caagagaacc ggaacacagg tgctgcatgg ctgtcgtcag ctcgtgtcgt gagatgttgg 1080

gttaagtccc gcaacgagcg caacccttgt cattagttgc tacgaaaggg cactctaatg 1140

agactgccgg tgacaaaccg gaggaaggtg gggatgacgt caagtcctca tggcccttat 1200

gggtagggct tcacacgtca tacaatggtc gggacagagg gtcgccaacc cgcgagggggg 1260

agccaatccc agaaacccga tcgtagtccg gatcgcagtc tgcaactcga ctgcgtgaag 1320

tcggaatcgc tagtaatcgc ggatcagcat gtcgcggtga atacgttccc gggtcttgta 1380

cacaccgccc gtcacaccat gggagtgggt tttaccagaa gtagttagcc taaccgnaag 1440

gggggcgatt accacggtag gattcatgac tggggtgaag tcgtaacaag gtagccgtat 1500

cggaaggtgc ggctggatca cctccttt 1528

<210> 123

<211> 1522

<212> DNA

<213> Microbacterium murale

<220>

<223> 16S

<400> 123

tacggagagt ttgatcctgg ctcaggatga acgctggcgg cgtgcttaac acatgcaagt 60

cgaacggtga acacggagct tgctctgtgg gatcagtggc gaacgggtga gtaacacgtg 120

agcaacctgc ccctgactct gggataagcg ctggaaacgg cgtctaatac tggatatgtg 180

acgtggccgc atggtctgcg tctggaaaga atttcggttg gggatgggct cgcggcctat 240

cagcttgttg gtgaggtaat ggctcaccaa ggcgtcgacg ggtagccggc ctgagagggt 300

gaccggccac actgggactg agacacggcc cagactccta cgggaggcag cagtggggaa 360

tattgcacaa tgggcgcaag cctgatgcag caacgccgcg tgagggatga cggccttcgg 420

gttgtaaacc tcttttagca gggaagaagc gaaagtgacg gtacctgcag aaaaagcgcc 480

ggctaactac gtgccagcag ccgcggtaat acgtagggcg caagcgttat ccggaattat 540

tgggcgtaaa gagctcgtag gcggtttgtc gcgtctgctg tgaaatccgg aggctcaacc 600

tccggcctgc agtgggtacg ggcagactag agtgcggtag gggagattgg aattcctggt 660

gtagcggtgg aatgcgcaga tatcaggagg aacaccgatg gcgaaggcag atctctgggc 720

cgtaactgac gctgaggagc gaaagggtgg ggagcaaaca ggcttagata ccctggtagt 780

ccaccccgta aacgttggga actagttgtg gggtccattc cacggattcc gtgacgcagc 840

taacgcatta agttccccgc ctggggagta cggccgcaag gctaaaactc aaaggaattg 900

acggggaccc gcacaagcgg cggagcatgc ggattaattc gatgcaacgc gaagaacctt 960

accaaggctt gacatatacg agaacgggcc agaaatggtc aactctttgg acactcgtaa 1020

acaggtggtg catggttgtc gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac 1080

gagcgcaacc ctcgttctat gttgccagca cgtaatggtg ggaactcatg ggatactgcc 1140

ggggtcaact cggaggaagg tggggatgac gtcaaatcat catgcccctt atgtcttggg 1200

cttcacgcat gctacaatgg ccggtacaaa gggctgcaat accgcgaggt ggagcgaatc 1260

ccaaaaagcc ggtcccagtt cggattgagg tctgcaactc gacctcatga agtcggagtc 1320

gctagtaatc gcagatcagc aacgctgcgg tgaatacgtt cccgggtctt gtacacaccg 1380

cccgtcaagt catgaaagtc ggtaacacct gaagccggtg gcctaaccct tgtggaggga 1440

gccgtcgaag gtgggatcgg taattaggac taagtcgtaa caaggtagcc gtaccggaag 1500

gtgcggctgg atcacctcct tt 1522

<210> 124

<211> 1537

<212> DNA

<213> Kluyvera intermedia

<220>

<223> 16S

<220>

<221> modified base

<222> (455)..(455)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (1004)..(1004)

<223> a, c, t, g, unknown or other

<400> 124

attgaagagt ttgatcatgg ctcagattga acgctggcgg caggcctaac acatgcaagt 60

cgaacggtag cacagagagc ttgctctcgg gtgacgagtg gcggacgggt gagtaatgtc 120

tgggaaactg cccgatggag ggggataact actggaaacg gtagctaata ccgcataatg 180

tcgcaagacc aaagaggggg accttcgggc ctcttgccat cggatgtgcc cagatgggat 240

tagcttgttg gtgaggtaat ggctcaccaa ggcgacgatc cctagctggt ctgaggat 300

gaccagccac actggaactg agacacggtc cagactccta cgggaggcag cagtggggaa 360

420

gttgtaaagc actttcagcg gggaggaagg cgatncggtt aataaccgtg ttgattgacg 480

ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat acggagggtg 540

caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtctgtc aagtcggatg 600

tgaaatcccc gggctcaacc tgggaactgc attcgaaact ggcaggcttg agtcttgtag 660

aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg aataccggtg 720

gcgaaggcgg ccccctggac aaagactgac gctcaggtgc gaaagcgtgg ggagcaaaca 780

ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt tgtgcccttg 840

aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg gccgcaaggt 900

taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga 960

tgcaacgcga agaaccttac ctggtcttga catccacgga attnggcaga gatgccttag 1020

tgccttcggg aaccgtgaga caggtgctgc atggctgtcg tcagctcgtg ttgtgaaatg 1080

ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg tccggccggg 1140

aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt caagtcatca 1200

tggcccttac gaccaggggct acacacgtgc tacaatggca tatacaaaga gaagcgacct 1260

1320

ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga atacgttccc 1380

gggccttgta cacaccgcc gtcacaccat gggagtgggt tgcaaaagaa gtaggtagct 1440

taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag tcgtaacaag 1500

gtaaccgtag gggaacctgc ggttggatca ccttcctt 1537

<210> 125

<211> 882

<212> DNA

<213> Kluyvera intermedia

<220>

<223> nifH

<400> 125

atgaccatgc gtcaatgcgc catttatggc aaaggtggga tcggcaaatc caccaccacg 60

caaaacctcg tcgccgctct cgcggaaatg ggtaaaaaag tgatgatcgt cggctgcgac 120

ccgaaagcgg actccaccg tctgatcctg catgcgaaag cacagaacac cattatggag 180

atggccgccg aagtgggttc agtggaagac cttgaactgg aagatgtgct gcaaatcggt 240

tacggcggcg tgcgttgtgc agaatccggc ggcccggagc caggcgtggg ttgtgcaggc 300

cgcggcgtta ttaccgccat taacttcctt gaagaagaag gcgcctatgt cagcgacctc 360

gactttgtct tctatgacgt cctcggtgac gtggtctgcg gcgggttcgc catgccgatt 420

cgtgaaaaca aagcgcaaga gatctatatc gtctgctccg gggaaatgat ggcgatgtat 480

gccgctaaca acatctccaa aggcatcgtg aaatacgcta aatccggcaa ggtgcgcctg 540

ggcgggctga tttgtaactc ccgtcagacc gaccgcgaag atgaactgat catcgcgctg 600

gcagaaaaac tgggcaccca gatgattcac tttgtgccac gcgacaacat cgtccagcgc 660

gcggaaattc gccgtatgac ggttatcgaa tatgacccga aatgcaacca ggccgacgaa 720

taccgcgcgc tggcgaacaa gatcgtcaac aacaccctga tggtcgtccc gaccccttgc 780

accatggatg aactggaaga gctgctgatg gaattcggca ttatggatgt ggaagacgcc 840

agcatcatcg gtaaaaccgc cgccgaagaa aacgcggcct ga 882

<210> 126

<211> 660

<212> DNA

<213> Kluyvera intermedia

<220>

<223> CDS of iron-molybdenum dinitrogenase cofactor

<400> 126

60

ctgcaacccg cgcagatcct ggcctggctg acaggagaac gcgacgacgc cttaaccccg 120

gcgtacctcg ataagcttaa cgtccgcgaa ctggaagcga ccttcccgtc tgaaacggcg 180

atgatgtcgc ccgcacgctg gagccgcgtt aacgcgtgcc ttcacggtac gctgcccgca 240

cacctgcagg taaaaagcac cactcgtcag gggcaattac gggtagcctt ttgttcacag 300

gatggattgc tgatcaatgg tcattttggt caggggcggc tgttttttat ctacgccttt 360

gatgaacagg gcggatggct acacgcgtta cgccgtcttc cctcggcccc gcaaacccag 420

gagccgaatg aagttcgcgc gcagctcctg agtgattgcc acctgctgtt ttgtgaagcc 480

attggcggcc ctgcggcggc ccggctgatt cgtcacaata tccacccgat gaaagtgtcg 540

ccagggatgt ccattgccgc ccagtgtgat gccattaccg cactgctgag cggacgtctg 600

ccaccgtggc tggcaaaacg tcttgagaaa gccaacccgc tggaagagcg ggtgttttaa 660

<210> 127

<211> 1374

<212> DNA

<213> Kluyvera intermedia

<220>

<223> nifD1

<400> 127

atgaagggaa atgacattct cgcgctgctg gatgaacccg cctgcgaaca caatcacaaa 60

cagaaatccg gctgtagcgc ccctaaaccc ggtgccacgg cgggcggttg cgcgttcgac 120

ggcgcgcaaa tcaccctgtt gccgctgtcg gatgtggcgc acctggtcca cggaccgatt 180

ggctgcacgg gaagctcctg ggataaccgg ggcagtatga gctccggccc cagtctcaac 240

cggctcggct ttaccaccga cctgaacgag caggatgtca ttatggggcg cggcgaacgg 300

cggcttttcc acgcggtgcg tcatatcgtc aaccgttatc accctgccgc cgtgtttatc 360

tataacacct gcgttccggc gatggagggt gatgatattg acgccgtctg tcaggcggcg 420

gaaaccgcca ccggcgtgcc agtgattgcc gttgatgccg ccgggttcta tggcagcaaa 480

aaccttggca accgtctcgc gggtgaagtg atggttaaca aggtcattgg acggcgcccg 540

cccgccccct ggccggacga tacccccttc gcgccggaac accgccacga tatcggcctg 600

attggcgaat ttaatatcgc cggggagttc tggcacgttc agccgctgct cgatgagctg 660

ggtattcgcg tgctggggcag cctttccgggg gatggccgtt ttagtgaaat ccagaccctg 720

caccacgcgc aggtcaatat gctggtctgc tcaagagcgc tgatcaatgt tgcccgcacc 780

ctggaacagc gctatggcac cccctggttt gagggcagtt tttacggcgt gcgcgctacc 840

tccgatgccc tgcgtcaact ggcatccctg cttggcgaca gcgatctgat tgcccgcacc 900

gaagccgtta ttgcccgcga agaagccacg gcaaatcagg cgctcgcccc gtggcgcgaa 960

cggctacagg gtcgcaaagt gctgctctat accggtgggg tgaaatcctg gtcggtggtc 1020

tccgcattgc aggatttagg gatgaccgtc gtggcgactg gcacccgcaa atctaccgaa 1080

gaagataagc agcgtattcg cgaattaatg ggcgatgacg cgctaatgct ggaagaaggc 1140

aacgcccgca ccctgctgga tgtggtgtac cgctatcagg cggatttgat gatcgctggg 1200

gggcgtaaca tgtataccgc gtacaaagcg cggctgccgt ttctggatat caaccaggag 1260

cgtgaacacg cctttgcggg ttatcgcggc atcgtcaccc tcgcccaaca gctttgccag 1320

actattgaaa gccccgtctg gccgcaaaca cacgcccgcg cgccgtggca ataa 1374

<210> 128

<211> 1449

<212> DNA

<213> Kluyvera intermedia

<220>

<223> nifD2

<400> 128

atgagcaatg caacaggcga acgtaatctg gaaattatcc aggaagtgct ggagatcttt 60

cccgaaaaaa cgcgcaaaga acgcagaaag cacatgatgg tgaccgaccc ggagatggaa 120

agcgtcggga aatgcatcat ctctaaccgc aaatcgcagc cgggtgtgat gactgtccgc 180

ggctgctcct acgccgggtc gaaaggcgtg gtttttggggc cgattaaaga tatggcccac 240

atctccccg gcccgatcgg ctgtgggcag tactcccgtg cggggcggcg caactactac 300

accggggtca gcggcgttga ttccttcggg acgctgaact ttacctctga ttttcaggag 360

cgcgatatcg tcttcggcgg cgataaaaag ctcaccaaac tgattgagga gatggaggaa 420

ctgttcccgc tgaccaaagg catctccatt cagtcggagt gcccggtagg tttaatcggt 480

gacgatatcg aagcggtggc gaatgccagt aaaaaagcgc tcaacaagcc ggtgatcccg 540

gtgcgttgcg aaggctttcg cggcgtgtcg cagtcgctcg gtcaccatat cgccaacgac 600

gttatccgcg actgggtgct ggataaccgc gaagggaagc ccttcgaatc taccccctat 660

gacgtggcca tcatcggcga ttacaacatc gggggggatg cctgggcgtc gcgcattctg 720

cttgaagaga tggggttacg cgtggtggcg cagtggtccg gtgacggcac gctggtagag 780

atggaaaaca ccccgttcgt caagctgaac ctggtgcact gctaccgctc tatgaactac 840

atctctcgcc atatggaaga gaaacacggt atcccgtgga tggagtacaa cttcttcggc 900

ccgaccaaaa tcgccgaatc gctgcgtaag atcgccgatc aatttgacga caccatccgc 960

gccaatgcgg aagcggtgat cgccaaatat caggcgcaaa acgatgcgat tatcgccaaa 1020

taccgcccgc gtctcgaagg ccgcaaggtg ctgctctata tgggtggcct gcgtcctcgc 1080

cacgtgattg gcgcgtatga ggatttgggc atggagattg tcgccgccgg gtatgaattt 1140

gcccataacg acgattacga ccgcaccctg ccggacctca aaggggcac gctgttgttc 1200

gacgatgcca gcagttatga actggaagcc ttcgtgaagg cgattaagcc ggacctcatt 1260

ggctcaggca tcaaggaaaa atacattttc cagaaaatgg gggtaccgtt tcgccagatg 1320

cactcctggg attactccgg cccgtatcac ggctatgacg gctttgccat ctttgcccgc 1380

gatatggaca tgacgctcaa caatcccgcc tggggcgagt tgaccgcacc ctggctgaaa 1440

tcagcctga 1449

<210> 129

<211> 1383

<212> DNA

<213> Kluyvera intermedia

<220>

<223> nifK1

<400> 129

atggcagata tcatccgtaa tcagaaaccg ctggcggtaa gcccggtaaa aagcggccag 60

ccgttaggcg ccattctggc gagcctcggc tttgagcaca gtattccact ggtgcacggt 120

gcgcagggat gcagcgcgtt cgccaaagtg ttttttatcc aacattttca tgaccctatt 180

ccgctgcaat ccacggcgat ggaccccacc tcaacggtca tgggggcgga cggcaatatc 240

cttgccgcgc tcaatacgct gtgccagcgc aacaccccga aagctatcgt cctgttgagt 300

accggcctgt ctgaggcgca gggcagcgat atcagccgcg tggtacgtca gtttcgtgag 360

gattttcccc gccacaaaaa tatcgccctc ctgacggtca acaccccggga tttttacggc 420

acgctggaga acggctttag tgcggtggtg gaaagcgtca tcgaacagtg ggtgccggaa 480

aagcctcagc atggcctgcg taaccggcgg gtcaacttgt tgttaagtca cctgctgacg 540

cccggtgatg ttgagttgct gcgcagctac gtggaggctt ttggcctgca accggtgatc 600

gtgccggatc tttcacagtc gctggatggt cacctggcaa gcggtgattt ttcgccggtc 660

actcagggggg gaacgcccct gcgcattatc gaacagatgg gacagagcct gtgcacgttt 720

gctattggcg tgtcgctgtc ccgtgcggca tcgctgctgg cacagcgtag ccgtggcgag 780

gtgatcgtgc ttccccatct gatgaccatg gaacattgcg accgttttat tcatcaactg 840

aagatcattt cggggcgcga ggttcccgcc tggattgagc gccagcgcgg acaattgcag 900

gatgcgatga tcgattgtca tatgtggttg caggataccc ggctcgcgct ggccgccgag 960

ggcgatctgc tggcgggctg gtgtgatttc gcccgtagcc agggcatgct ccccggcccc 1020

gttgtggcgc cggtcagcca gccgggcctg caacagcttc ccgtggagaa agtggtcatt 1080

ggcgatctgg aagatatgca ggatttactc tgcgctatgc ctgctgacct gctggtcgcc 1140

aactcccatg ccgcagacct ggccgaacaa ttctccatcc cgctgatccg cgccggggttc 1200

cctatcttcg acaggcttgg cgaatttcgt cgcgtgcgtc agggataccc cggcattcgc 1260

gacacgctgt ttgagctggc gaacctgatg cgcgaacgtc atcaccacct gcccgtctac 1320

cgctcccccc tgcgccagca atttgcccag gacgctgacg gaggccgcta tgcaacatgt 1380

taa 1383

<210> 130

<211> 1563

<212> DNA

<213> Kluyvera intermedia

<220>

<223> nifK2

<400> 130

atgagccaaa ctgctgagaa aattgtcacc tgtcatccgc tgtttgaaca ggacgaatac 60

cagacgctgt ttcgcaataa gcgcggtctg gaagaggcgc acgacccgca gcgcgtgcaa 120

gaggtttttg aatggaccac cacggcggag tatgaagcgc tgaactttaa gcgtgaagcg 180

ttaaccgtcg atccggcaaa ggcctgccag cctttaggat cggtactctg ctcgctgggt 240

tttgccaata cgctgcctta tgtgcacggt tcccagggct gtgtggccta tttccgcacc 300

tattttaacc gtcatttcaa agagccgatc gcttgcgttt ccgactctat gacagaggat 360

gcggcggtct tcggcggcaa caacaacctt aacaccgggt tgcaaaatgc cagcgccctg 420

tacaaaccgg aaattgtcgc tgtctccact acctgtatgg cggaggtcat cggcgatgac 480

ctgcaggcct ttatcgccaa cgccaaaaag gacgggttta ttgatgccgc cattccggtg 540

ccctacgccc atacgccaag ttttatcggt agccacatca ccggctggga caacatgttt 600

gaaggtttcg ccggggcatt taccgccgat cacgtggcgc aaccggggcaa actggcgaag 660

ctaaacctgg tgaccggttt tgaaacctat cttggcaatt accgcgtgct caaacgcatg 720

atggcccaga tggaggtgcc ctgtagcctg ctgtctgacc cgtctgaggt gttagatacg 780

ccagccgacg gccactatcg catgtatgcg ggcggcacaa cgcaacaaga gatgcgcgac 840

gcccccgatg ctatcgacac cctgctgctg caaccctggc atctggtgaa gagtaaaaaa 900

gtggtgcagg agtcctgggg ccagcccgcc acagaagtgt ccatcccaat gggactgacc 960

gggaccgacg aactgctgat ggcagtcagt cagttaaccg gcaaaccggt ggccgatgaa 1020

ctgacgctgg agcgtgggcg cctggtggat atgattctcg attcacacac ctggctgcac 1080

ggtaagaaat tcggtctcta cggcgatccg gattttgtga tggggctgac gcgtttcctg 1140

ctggaactgg gctgcgagcc gacggttatc ctctgtcata acggtagcaa gcgctggcag 1200

aaagcgatga agaaaatgct tgaggcatcg ccctacggtc aggagagcga agtgttcatc 1260

aactgcgatc tgtggcattt ccgctcgctg atgtttaccc gcaaaccggga ctttatgatc 1320

ggcaactcgt acgccaaatt catccagcgt gacacgctgg cgaaaggcga acagtttgaa 1380

gttccgctga tccgtcttgg cttcccgttg ttcgaccgcc accacctgca tcgccagacc 1440

acatggggtt atgaaggggc gatgaatatc gtcaccaccc tggtcaacgc cgtgctggaa 1500

1560

taa 1563

<210> 131

<211> 1488

<212> DNA

<213> Kluyvera intermedia

<220>

<223> nifL

<400> 131

atgaccttta atatgatgct ggagaccagc gcaccgcagc acattgcggg caacctctca 60

cttcaacatc ccggactgtt ttccacgatg gttgaacagg ctccgatcgc gatttcgctg 120

accgacccgg acgcgaggat tctgtacgct aatccggcct tttgtcgcca gaccggttat 180

agcctggaag agctgctcaa ccagaaccat cgcatactgg caagccaaca gacgccgcgc 240

agcatttatc aggaactgtg gcaaacgctg ctgcaacaga tgccctggcg cggtcagctc 300

atcaatcgcc gtcgggatgg cagcctttat ctggctgagg tcgatatcac cccggtcgtc 360

aacaaacagg gcgaactgga acactacctc gccatgcaac gtgatatcag cgccagctat 420

gcgctcgaac agcgattgcg caatcacacc accatgagcg aggcggtgct gaacaacatt 480

cctgccgccg tggtggtggt caacgagcag gaccaggtag tcatggacaa cctcgcctac 540

aaaaccttct gtgccgactg cggtggcaag gagctgctca ccgaactgga tttctcccgg 600

cgcaaaagcg atctctatgc cgggcaaata ctgcctgtgg tgctgcgcgg cgccgtgcgc 660

tggctctctg tcacctgctg gaccttgccg ggggtgagcg aagaagccag ccgctacttt 720

attgataccg cgctgccccg caccctggtg gtgatcaccg actgcaccca gcaacaacaa 780

caggccgaac agggccgtct cgatcgtctc aaacaggaga tgaccaccgg gaagctgctg 840

gccgcgatcc gtgaatcgtt ggatgccgcg ctggttcagc taaactgccc catcaatatg 900

ctggcggcgg cgcgacgtct caacggtgaa gataaccata acgtggcgct ggatgccgcg 960

tggcgcgagg gggaagaggc gctggcccgc ctgcaacgct gccgcccttc tctcgatctg 1020

gaagagagcg cgctgtggcc tctgcaaccg ctgtttgacg acctgcgcgc cctttaccat 1080

acccgctata acaatggcga aaatctgcac gttgaaatgg cctctccgca tctggcgggg 1140

tttggtcagc gcacgcagat ccttgcctgt ctcagtttgt ggctcgaccg tacgctggcc 1200

ctcgccgccg cgctaccggga cagaacgctg catacccagc tttacgcccg tgaagaagat 1260

ggctggctgt ccatttggct gacagataat gtgccgctca tccatgtgcg atacgcccac 1320

1380

gttgcccatc atcgcggcgc aatagaacta actacccgcc ctgaaggcgg tacctgcctg 1440

accctgcgat tcccgttatt tcattcactg accggaggcc cacgatga 1488

<210> 132

<211> 1572

<212> DNA

<213> Kluyvera intermedia

<220>

<223> nifA

<400> 132

atgacccagc gacccgagtc gggcaccacc gtctggcgtt ttgatctctc acagcaattt 60

accgccatgc agcgcatcag cgtggtgttg agtcgcgcaa ccgagataag ccagacgctg 120

caggaggtgc tgtgtgttct gcataatgac gcatttatgc aacacggcat gctgtgtctg 180

tatgacaacc agcaggaaat tctgagtatt gaagccttgc aggaggcaga ccaacatctg 240

atccccggca gctcgcaaat tcgctatcgc cctggcgaag ggctggtagg agccgtactg 300

tcccagggac aatctcttgt gctgccgcgt gtcgccgacg atcaacgctt tctcgacagg 360

cttggcatct atgattacaa cctgccgttt atcgccgtcc ccttaatggg gccaggcgcg 420

cagacgattg gcgtgctcgc cgcgcagccg atggcgcgtc tggaggagcg gcttccttcc 480

tgtacgcgct ttctggaaac cgtcgccaat ctggtcgcac agacagtccg gctgatgacc 540

ccgcctgccg ccgccacacc gcgcgccgcg attgcccaga ccgaacgcca gcgcaactgt 600

ggcactcctc gccccttcgg ctttgagaat atggtgggca aaagcccggc catgcagcag 660

acaatggaca ttatccgcca ggtttcgcgc tgggatacca cggtactggt gcgcggcgaa 720

agcggcaccg gtaaagaact tatcgccaat gctattcatc acaactcccc tcgcgccgcc 780

gcgccctttg tgaaatttaa ctgcgcggcg ctaccggata cgctactgga gagcgaattg 840

ttcggccatg aaaaaggggc gttcaccggc gcggttcgcc agcgtaaagg acgttttgaa 900

ctggccgatg gcggcacact gtttcttgat gaaattggcg aaagcagcgc ctcgttccag 960

gccaaactgc tgcgtatttt gcaggagggt gaaatggagc gcgttggcgg cgacgaaacc 1020

ctgcgcgtca atgtgcgtat catcgccgcc accaaccgga atctggaaga agaggtgcgg 1080

atgggcaatt tccgcgagga tctctattat cgcctcaacg taatgcccat ctccctgccc 1140

ccgctgcgtg aacgtcagga ggacattgcc gagctggcgc actttctggt gcgcaaaatc 1200

gcccataacc aggggcgtac gctgcgcatc agtgatggcg ccatccgtct gctgatgggt 1260

tacaactggc ccggtaacgt gcgtgagctg gaaaattgcc tggaacgttc ggcagtgatg 1320

tcagaaaacg gcctgatcga ccgcgatgtg gtgctcttta accaccgtga gaacacgcca 1380

aaactcgcta tcgccgccgc gccaaaagag gatagctggc ttgatcaaac gctggatgaa 1440

1500

1560

cccaggatgt ga 1572

<210> 133

<211> 2853

<212> DNA

<213> Kluyvera intermedia

<220>

<223> glnE

<400> 133

atgatgccgc actctccaca gctacagcag cactggcaaa ctgtactggc ccgcttgcct 60

gagtcattca gtgaaacacc gcttagtgaa caagcgcagt tagtgcttac tttcagtgat 120

tttgtgcagg atagccttgc cgcgcatcct gactggctgg ctgagctgga aagcgcaccg 180

ccacaggcgg acgagtggaa gcagtatgcg caaacccttc gcgaatcgct ggaaggtgtg 240

ggagatgagg catcattaat gcgtgcgctg cgcctgttcc gtcgccatat gatggtgcgc 300

attgcctggg cgcagtcgct ggcgctggtg gcagaagatg agacgttgca gcagttgagc 360

gtactggcgg agaccctgat cgtcgctgca cgcgactggc tttacgatgc ctgctgtcgc 420

gagtggggaa cgccgtgcaa tcagcagggg gaaccgcagc cgttgctgat cctgggcatg 480

ggcaagctgg gtggcggggga gcttaacttt tcgtccgata tcgatctgat ttttgcctgg 540

cgggaaaacg gttcaacgcg cggtgggcga cgcgaacttg ataacgccca gttttttact 600

660

cgcgtggata tgcggctgcg cccgtttggc gacagcggtc cgctggtgct gagttttgcc 720

gcgctggaag attattatca ggagcagggg cgcgactggg aacgttatgc gatggtgaaa 780

gcccgcatta tgggcgataa ggacgatgtt tacgctggcg aattacgggc catgctgcgg 840

ccgttcgtct tccgtcgcta tatcgatttc agcgttattc agtctctgcg taacatgaaa 900

gggatgattg cccgcgaagt gcgccgccgt ggtctgaaag ataacattaa gctgggcgcg 960

ggcggcatcc gtgagattga gtttatcgtt caggtgttcc agttgatacg cggtgggcgc 1020

1080

ttgctgccgc ctggcgatgc accggcgtta cgccaggcct atttgtatct gcgccgtctg 1140

gaaaacctgc tgcaaagcat taacgacgaa caaacgcaga cgctgccgac agatgaactc 1200

aatcgcgcgc gtctggcctg ggggatgcgg gtcgcagact gggaaaccct gaccgctgag 1260

cttgaaaagc agatgtctgc cgtacgaggg atattcaaca ccctgattgg cgatgacgaa 1320

gccgaagagc agggggatgc gctctgcggg caatggagtg agttgtggca ggatgcgttt 1380

caggaagatg acagcacgcc tgtgctggcg cacctttctg acgatgatcg ccgccgcgtg 1440

gtcgcgatga ttgctgattt tcgcaaagag ctggataaac gcaccattgg cccacgcggc 1500

cgccaggtgc tcgaccatct gatgccgcat ctgttgagtg atgtctgctc ccgtgaggat 1560

gcccctgtac cgttgtctcg cgtgacgccg ctgttaacgg gaattgtcac gcgtacgacg 1620

tatcttgagc tgctcagcga gtttcctggt gcgcgtaagc atctgatttc actctgtgcc 1680

gcctcgccga tggtggccag taagctggcg cgctatccgt tattgctgga tgagttgctc 1740

gatccgaata ccctttatca gcccacggcg atgaatgcct accgggatga gctacgtcag 1800

1860

aaacaggctc aattgttgcg tgtggcggca gcagatctgg caggcacact ccccgtgatg 1920

aaagtgagcg atcacttaac atggcttgcc gaagccatca ttgaagccgt ggtacaacag 1980

gcgtggagcc tgatggtatc gcgttatggg cagccgaaac acttacgcga ccgtgaaggc 2040

cgtgggtttg cagtggtcgg ttacggcaaa ctgggcggtt gggagctggg ctatagttcc 2100

gatctggatt tgattttcct tcatgactgt ccggtggacg tgatgactga cggcgagcgg 2160

gaaatcgatg gccgccaatt ttatctgcgc cttgcccagc gcgtgatgca cctgttcagt 2220

acgcgcacct catccgggat cctgtatgag gtagacgcgc gcttgcgccc gtccggtgcg 2280

gcgggaatgc tggtgacctc aaccgaatcc tttgccgact accagcgcac cgaagcctgg 2340

acctgggaac atcaggcgct ggttcgcgcc cgcgttgtct atggcgatcc acaattaaac 2400

gcgcaatttg atgccatccg ccgcgatatc accatgaccg tgcgtaatgg tgcaacgtta 2460

2520

gatcgctttg atattaaagc cgatgagggt ggaattaccg atatcgaatt tatcacccag 2580

tatctggtgc tgcgttatgc ccatgccaaa ccgaaactga cgcgctggtc ggacaatgtc 2640

cgcattctgg aagggctggc gcaaaacggc attatggaag agcaggaagc gcaggcactt 2700

accaccgcct atacaacgtt gcgtgatgag ctgcatcacc tggcgctaca ggagctgcca 2760

ggacatgttc cggaggcatg ttttgtcgct gaacgcgcga tggtgcgagc ctgctggaac 2820

aagtggttgg tggagccgtg cgaggacgcg taa 2853

<210> 134

<211> 1290

<212> DNA

<213> Kluyvera intermedia

<220>

<223> amtB

<400> 134

atgaagaaag cactattaaa agcgggtctg gcctcgctgg cattactgcc gtgtctggct 60

atggcagccg atccggttgt cgtcgataaa gccgacaatg cctttatgat gatttgcacc 120

gcgctggtgc tgtttatgtc aattccgggc atcgccctgt tctatggtgg tttaatccgc 180

ggtaaaaacg tcctttctat gctgacacag gttgcggtta cgttcgcact ggtgtgcgtg 240

ctgtgggtgg tttacggcta ctctctggcc tttggcactg gcggcagctt cttcggtagc 300

ttcgactggg tgatgctgaa aaatattgag ctgaaagcgc tgatgggcac catctatcag 360

tacattcacg ttgcgttcca gggctcgttt gcctgtatta ccgtcggcct gattgtcggt 420

gcgctggcag aacgtatccg tttctccgca gtactgattt tcgtcgtggt atggctgacg 480

ctgtcctacg tgccgatcgc acacatggtc tggggcggcg gtctgctggc aacccatggc 540

gccatggatt ttgcgggcgg tacagtcgtt cacatcaacg cagccgttgc aggcctggtg 600

ggtgcttacc tgattggcaa acgtgtcggt ttcggtaaag aagcgtttaa accgcacaac 660

ctgccgatgg tgtttaccgg tacggcaatc ctctactttg gctggttcgg attcaacgcg 720

ggttctgcaa gcgcggcgaa cgaaattgcg ggtctggctt ttgttaacac cgtcgtggca 780

acagcgggtg caatcctctc ctgggtcttc ggtgagtggg cgctgcgcgg caaaccgtct 840

ctgttgggtg cctgttctgg tgcgattgct ggcctcgtgg gtatcacccc ggcgtgtggt 900

tacgttggtg tgggtggcgc gctgatcgtg ggcatcgttg caggcctggc gggtctgtgg 960

ggcgttaccg cgctgaaacg ctggctgcgt gttgacgacc cgtgtgatgt cttcggtgtt 1020

cacggcgtgt gcggtatcgt aggttgtatc atgacaggta tcttcgcagc cacttcactg 1080

ggcggcgtgg gttatgccga aggcgtgacc atgggccatc aggttctggt acaactggaa 1140

agtatcgcca ttactatcgt atggtctggt atcgtcgcct ttatcggtta caaactggct 1200

gatatgacag tgggtctgcg tgttccggaa gatcaggaac gcgaagggct ggacgtcaac 1260

agccacggcg agaacgccta caacgcctga 1290

<210> 135

<211> 498

<212> DNA

<213> Kluyvera intermedia

<220>

<223> PinfC

<400> 135

ctggggtcac tggagcgctt tatcggcatc ctgaccgaag aatttgccgg tttcttcccg 60

acctggctgg cccctgttca ggttgtggtg atgaatatca ctgattctca agctgaatat 120

gtcaacgaat tgacccgtaa attgcaaaat gcgggcattc gtgtaaaagc ggacttgaga 180

aacgagaaga ttggctttaa aatccgcgag cacactttac gtcgtgtccc ttatatgttg 240

gtctgtggtg ataaagaggt ggaagcaggc aaagtggccg ttcgcacccg ccgcggtaaa 300

gacctgggca gcctggacgt aagtgaagtg attgagaagc tgcaacaaga gattcgcagc 360

cgcagtcttc aacaactgga ggaataaggt attaaaggcg gaaaacgagt tcaaacggca 420

cgtccgaatc gtatcaatgg cgagattcgc gccaggaag ttcgcttaac tggtctggaa 480

ggtgagcagc tgggtatt 498

<210> 136

<211> 1537

<212> DNA

<213> Kosakonia pseudosacchari

<220>

<223> 16S

<220>

<221> modified base

<222> (454)..(454)

<223> a, c, t, g, unknown or other

<400> 136

attgaagagt ttgatcatgg ctcagattga acgctggcgg caggcctaac acatgcaagt 60

cgaacggtag cacagagagc ttgctctcgg gtgacgagtg gcggacgggt gagtaatgtc 120

tgggaaactg cctgatggag ggggataact actggaaacg gtagctaata ccgcataacg 180

tcgcaagacc aaagaggggg accttcgggc ctcttgccat cagatgtgcc cagatgggat 240

tagctagtag gtggggtaac ggctcaccta ggcgacgatc cctagctggt ctgagaggat 300

gaccagccac actggaactg agacacggtc cagactccta cgggaggcag cagtggggaa 360

tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga aggccttcgg 420

gttgtaaagt actttcagcg gggaggaagg cganacggtt aataaccgtg ttgattgacg 480

ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat acggagggtg 540

caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtctgtc aagtcggatg 600

tgaaatcccc gggctcaacc tgggaactgc atccgaaact ggcaggcttg agtctcgtag 660

agggaggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg aataccggtg 720

gcgaaggcgg ccctcctggac gaagactgac gctcaggtgc gaaagcgtgg ggagcaaaca 780

ggattagata ccctggtagt ccacgccgta aacgatgtct atttggaggt tgtgcccttg 840

aggcgtggct tccggagcta acgcgttaaa tagaccgcct ggggagtacg gccgcaaggt 900

taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga 960

tgcaacgcga agaaccttac ctggtcttga catccacaga acttgccaga gatggcttgg 1020

tgccttcggg aactgtgaga caggtgctgc atggctgtcg tcagctcgtg ttgtgaaatg 1080

ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg tccggccggg 1140

aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt caagtcatca 1200

tggcccttac gaccaggggct acacacgtgc tacaatggcg catacaaaga gaagcgacct 1260

1320 cgcgagagca agcggacctc ataaagtgcg tcgtagtccg

ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga atacgttccc 1380

gggccttgta cacaccgcc gtcacaccat gggagtgggt tgcaaaagaa gtaggtagct 1440

taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag tcgtaacaag 1500

gtaaccgtag gggaacctgc ggttggatca ccttcctt 1537

<210> 137

<211> 882

<212> DNA

<213> Kosakonia pseudosacchari

<220>

<223> nifH

<400> 137

atgaccatgc gtcaatgcgc catttacggc aaaggtggga tcggtaaatc gaccaccaca 60

cagaacctgg tcgccgcgct ggcggagatg ggtaagaaag tcatgatcgt cggctgcgat 120

ccgaaagccg actccacgcg tttgatcctg catgcgaaag cgcagaacac cattatggag 180

atggccgccg aagtcggctc cgtcgaagac ctggaattag aagacgtgct gcaaatcggt 240

tacggcggcg tgcgctgcgc ggaatccggt ggcccggagc caggtgtggg ttgtgccggt 300

cgtggcgtga tcaccgcgat taacttcctc gaagaagaag gcgcttacgt gccggatctg 360

gtggttgcg gtggtttcgc catgccgatt 420

cgtgaaaaca aagcgcagga gatctacatc gtttgctctg gcgaaatgat ggcgatgtac 480

gccgccaata acatctccaa aggcatcgtg aaatatgcca aatccggtaa agtgcgcctc 540

ggcgggctga tttgtaactc gcgccagacc gaccgcgaag atgaactcat cattgcgctg 600

gcggaaaaac tcggcacgca aatgatccac tttgttcccc gcgacaacat tgtgcagcgt 660

gcggaaatcc gccgtatgac ggttatcgaa tatgacccga cctgcaatca ggccaacgaa 720

tatcgcagcc ttgccagcaa aatcgtcaac aacaccaaaa tggtggtacc aaccccctgc 780

accatggatg aactggaaga actgctgatg gagttcggca ttatggatgt ggaagacgcc 840

agcatcattg gtaaaaccgc cgccgaagaa aacgccgtct ga 882

<210> 138

<211> 1449

<212> DNA

<213> Kosakonia pseudosacchari

<220>

<223> nifD1

<400> 138

atgagcaatg caacaggcga acgtaacctg gaaatcatcg agcaggtgct ggaggttttc 60

cgggaaaaga cgcgcaaaga gcgcagaaaa cacatgatgg tgacggaccc ggagcaggag 120

agcgtcggca agtgcatcat ctctaaccgc aaatcgcagc cgggcgtgat gaccgtgcgt 180

ggctgctcgt atgccggatc aaaaggggtg gtatttgggc caatcaaaga tatggcgcat 240

atctccccg gcccgatcgg ctgcgggcag tactcccgcg ccgggcggcg taactactat 300

accggcgtca gcggcgtgga cagtttcggc acgctcaact tcacctccga tttccaggag 360

cgcgacatcg tgtttggcgg cgacaaaaag ctcgccaaac tgattgaaga gctggaagaa 420

ctgtttccgc tgaccaaagg catttcgatt cagtcggaat gcccggtcgg cctgattggc 480

gatgatattg aagccgtggc gaacgccagc cgcaaagcga tcaacaaacc ggttattccg 540

gtgcgttgcg aaggctttcg cggcgtgtcg caatccctcg gtcaccatat tgccaacgat 600

gtgatccgcg actgggtact ggataaccgc gaaggcaaac cgtttgaatc caccccttac 660

gatgtggcga tcatcggcga ttacaacatc ggtggcgacg cctgggcctc gcgcattttg 720

ctcgaagaga tggggttgcg ggtggtcgcg cagtggtccg gcgacggtac gctggtggag 780

atggaaaaca cgccgttcgt caaactgaac ctggtgcact gctaccgctc gatgaactac 840

atctcgcgcc atatggagga gaagcacggt attccgtgga tggaatacaa cttctttggc 900

ccgacgaaaa tcgcggaatc gctgcgcaaa atcgccgacc tgttcgacga caccattcgc 960

gccaacgccg aagcggtgat cgcccgatac caggcgcaga acgacgccat tatcgccaaa 1020

tatcgcccac gtctggaggg tcgcaaagtg ttgctctata tgggcgggct gcgtccgcgc 1080

catgtgattg gcgcctatga agatctggga atggagatca tcgccgccgg ttatgagttt 1140

ggtcataacg acgattacga ccgcaccctg ccggatctga aagagggcac gctgctgttt 1200

gatgacgcca gcagctatga gctggaggcg tttgtcaacg cgctgaaacc ggatctcatc 1260

ggttccggca tcaaagagaa gtacatcttt cagaaaatgg gcgtgccgtt tcgccagatg 1320

cactcctggg attactccgg cccgtaccac ggctatgacg gcttcgccat cttcgcccgc 1380

gatatggata tgacgctcaa caaccccgcc tggggtcagt tgaccgcgcc gtggcttaaa 1440

tccgcctga 1449

<210> 139

<211> 1374

<212> DNA

<213> Kosakonia pseudosacchari

<220>

<223> nifD2

<400> 139

atgaagggga acgacatcct ggctctgctc gatgaaccag cctgcgagca taaccataaa 60

cagaaaaccg gctgtagcgc gccaaaaccc ggcgccaccg cgggaggctg cgccttcgac 120

ggcgcacaga tcaccctgct gccactttcc gatgtggcgc atctggtaca tggcccgatt 180

ggctgcgccg gcagctcatg ggataaccgt ggcagcctga gttctggccc gctgattaac 240

cgactcggat tcaccactga tttgaacgaa caggatgtca tcatggggcg cggcgagcgg 300

cggttgtttc acgcggtgcg ccatattgtc gagcgctatc acccggcggc ggtatttatt 360

tacaacacct gcgttccggc tatggaaggc gatgacattg acgcggtctg ccaggccgcc 420

gcgaccgcca ccggtgtgcc cgtgattgcc gtagatgtgg ccggttttta cggtagcaaa 480

aacctgggta accgcctcgc gggcgaggtg atggtgaaaa aagttatcgg cgggcgcgaa 540

cccgcgccgt ggccggacaa tacacctttt gccccggcgc accgccatga cataggcctg 600

attggcgaat ttaacatcgc cggcgagttc tggcatatcc agccgctgct tgatgagctg 660

ggtattcgcg tccttggctc cctttccggc gacgggcgct ttgccgagat cggacgttg 720

caccgcgcgc aggtcaatat gctggtgtgc tccagggcgc tgattaatgt cgccagatcg 780

cttgaacaac gttatggcac accctggttt gaaggcagtt tttatggcgt tcgcgccacc 840

tccgatgccc tgcgccagct ggcaacactc accggcgata gcgatttaat ggcgcgaacc 900

gaacggctga tcgcacgtga agagcaagcc acagaacagg cgctagcacc gctgcgtgaa 960

cggttacacg gccggaaagt gctgctctat accggtggcg tgaaatcctg gtcggtggtt 1020

tcggcgctgc aggatctcgg catgacggtc gttgctaccg gaacgcgcaa atccaccgaa 1080

gaggataaac aacgcatccg tgaactgatg ggcgatgacg ccatcatgct ggatgaaggc 1140

aatgcccgcg ccttgctgga tgtggtctat cgctacaaag ccgacatgat gatcgcgggc 1200

gggcgcaaca tgtacaccgc ctataaagcg cgtctgccct ttctggatat caaccaggag 1260

cgtgaacacg cgtttgccgg ttatcgcggc atcatcacgc ttgccgaaca actttgtcag 1320

acgctggaaa gcccggtctg gccgcaaaca catgcccgcg ccccgtggca ataa 1374

<210> 140

<211> 1563

<212> DNA

<213> Kosakonia pseudosacchari

<220>

<223> nifK1

<400> 140

atgagccaga ctgctgagaa aatacagaat tgccatcccc tgtttgaaca ggacgcctac 60

cagacactat ttgccggtaa acgggcactc gaagaggctc actcgccgga gcgggtgcag 120

gaagtgtttc aatggaccac caccccggaa tacgaagcgc tgaacttcaa acgcgaagcg 180

ctgactatcg acccggcaaa agcctgccag ccgctggggg cggtgctctg ttcgctgggg 240

tttgccaaca ccctgccgta tgtgcacggt tcacaggggtt gtgtggccta tttccgtacg 300

tactttaacc gccacttcaa agaaccggtg gcctgcgtgt cggattcgat gacggaagac 360

gcggccgtgt tcggcgggaa taacaacctc aacaccgggt tacaaaacgc cagcgcactg 420

tataaaccgg agattatcgc cgtctctacc acctgtatgg cggaagtgat cggtgatgat 480

ttacaggcgt ttatcgccaa cgccaaaaaa gatggttttc tcgatgccgc catccccgtg 540

ccctacgccc acacccccag ttttatcggt agccatatca ccggctggga caacatgttt 600

gaaggttttg cccgtacctt taccgcaaac catcagccac agcccggtaa actttcacgc 660

ctgaacctgg tgaccgggtt tgaaacctat ctcggcaatt tccgcgtgct gaaacgcatg 720

atggaacaaa tggaggtgca ggcgagtgtg ctctccgatc cgtcggaggt gctggacacc 780

cccgccaatg gccattacca gatgtacgcg ggcggtacga cgcagcaaga gatgcgcgag 840

gcaccggatg ccatcgacac cctgctgctg caaccgtggc agctggtgaa aagcaaaaaa 900

gtggtgcagg agatgtggaa tcagcccgcc accgaggttg ccattcccgt cgggctggca 960

ggcacagacg aactgttgat ggcgattagc cagttaaccg gcaaagccat tcccgattcg 1020

ctggcgctgg agcgcgggcg gctggtcgat atgatgctcg actcccacac ctggttacac 1080

ggtaaaaaat tcggtctgtt tggcgatccg gattttgtca tgggattgac ccgcttcctg 1140

ctggaactgg gctgtgaacc tgccgtcatc ctctgccata acggtaacaa acgctggcaa 1200

1260

aactgcgact tgtggcattt ccgctcgctg atgttcaccc gccagccgga ttttatgatt 1320

ggcaactcgt acgccaagtt tattcagcgc gacaccttag ccaagggcga acagtttgaa 1380

gtcccgctga tccgcctcgg ttttccgctg ttcgaccgtc accatctgca ccgccagacc 1440

acctggggct acgagggcgc gatgagcatt ctcacgacgc tggtgaatgc ggtactggag 1500

1560

taa 1563

<210> 141

<211> 1386

<212> DNA

<213> Kosakonia pseudosacchari

<220>

<223> nifK2

<400> 141

atggctgata ttgttcgtag taaaaaaccg ctggcggtga gcccgataaa aagcggccag 60

ccgctggggg cgatcctggc aagcctgggt ttcgaacagt gcataccgct ggtacacggc 120

gctcaggggt gcagcgcgtt cgcgaaagtg ttcttttattc aacattttca cgacccgatc 180

ccgctgcaat cgacggcgat ggacccgact tccaccatta tgggcgccga tgaaaacatt 240

tttaccgcgc tcaatgttct ctgccagcgc aacgccgcga aagccatcgt gctgctcagc 300

accgggctgt cagaagccca gggcagcgat atttcacgag tggtgcgcca gtttcgtgat 360

gactttccgc ggcataaaaa cgtggcgctg ctcaccgtca acaccccggga tttctacggc 420

tcgctggaaa acggctacag cgccgtgctg gaaagcatga ttgaacagtg ggtgcccgcg 480

cagcccgccg ccagcctgcg caaccgtcgc gtcaacctgc tggtcagcca tttactgacg 540

ccgggcgata tcgaactgtt acgcagttat gtggaagcat tcggtctgca accggtgatt 600

gtgccggatc tatcgcagtc gctggacgga catctggcca acggtgattt ttcgcccgtc 660

acccagggggg gaacaccgct gcgcatgatt gaacagatgg ggcaaaacct ggccactttt 720

gtgattggcc actcgctggg gcgggcggcg gcgttactgg cgcagcgcag ccgtggcgag 780

gtgatcgccc tgccgcatct gatgacgctt gatgcgtgcg acacctttat ccatcgcctg 840

aaaaccctct ccgggcgcga cgtgcccgcg tggattgagc gccagcgcgg gcaagtgcag 900

gatgcgatga tcgattgcca tatgtggttg cagggcgcgg ctatcgccat ggccgcagaa 960

ggcgatcacc tggcggcatg gtgcgatttc gcccgcagcc agggcatgat ccccggcccg 1020

gttgtcgcgc cggtcagcca gccggggttg caaaatctgc cggttgaaat ggtggtcatc 1080

ggcgatctgg aagatatgca ggatcggctt tgcgcgacgc ccgccgcgtt actggtggcc 1140

1200

ccggtgtatg accggctggg ggaatttcgt cggctgcgcc aggggtatag cggcattcgt 1260

gacacgctgt ttgagctggc gaatgtgatg cgcgaacgcc attgcccgct tgcaacctac 1320

cgctcgccgc tgcgtcagcg cttcggcgac aacgttacgc caggagatcg gtatgccgca 1380

tgttaa 1386

<210> 142

<211> 1488

<212> DNA

<213> Kosakonia pseudosacchari

<220>

<223> nifL

<400> 142

atgaccctga atatgatgat ggatgccagc gcgcccgagg ccatcgccgg tgcgctttcg 60

caacaacatc ctgggctgtt ttttaccatc gttgaagaag cccccgtcgc tatttcacta 120

accgatgccg aggcacgtat tgtctatgcc aacccggcat tctgccgcca gaccggctat 180

gagcttgagg agttgttgca gcaaaatccc cgcctgcttg ccagtcagca gaccccacgg 240

gaaatctacc aggatatgtg gcacaccctg ttacaacgtc gaccatggcg cgggcaattg 300

atcaaccgcc accgtgacgg cagccttttt ctggttgaga tcgatatcac cccggtgatt 360

aacccgtttg gcgaactgga acactacctg gccatgcagc gcgatatcag cgccggttat 420

gcgctggagc agcggttgcg taatcacatg gcgctgaccg aagcggtgct gaataacatt 480

ccggcggcgg tggtcgtggt cgatgaacgc gatcgtgtgg ttatggataa cctcgcctat 540

aaaactttct gtgctgattg cggcggaaaa gagctactga gcgaactcca tttttcagcc 600

660

tggttgtcgg tcacctgctg ggcgctgcca ggcgtcagcg aagaagccag tcgctacttt 720

attgataata ccttgacgcg cacgctggtg gtcatcaccg acgacaccca gcagcgccag 780

cagcaagagc aaggacggct tgaccgcctt aaacagcaga tgaccagcgg caaactgctg 840

gcggcgatcc gcgaagcgct tgacgccgcg ctgatccagc ttaactgccc catcaatatg 900

ctggcggcgg cgcggcgttt aaacggcagt gataacagca acgtagcgct ggacgccgcg 960

tggcgcgaag gtgaagaagc gatggcgcgg ctgaaacggt gccgcccgtc gctggagctg 1020

gaaagtgccg ccgtctggcc gctgcaaccc ttttttgacg acttgcgcgc gctttatcac 1080

acccgctacg agcagggtaa aaatttgcag gtcacgctgg attcgacgca tctggtggga 1140

tttggtcagc gaacccaact gctggcctgc ctgagtctgt ggctcgatcg cacgctggat 1200

1260

ggctggctct cgttgtatat cactgacaat gtgccgttga ttccgctgcg ccatacccat 1320

1380 tcgccggatg cgcttaacgc accgggaaaa ggtatggagt

gtagcgcatc acaacggcgc gatagaactc acttcacgcc ccgaaggggg aagctgcctg 1440

accctacgat tcccgctatt tcattcactg accggaggtt caaaatga 1488

<210> 143

<211> 1575

<212> DNA

<213> Kosakonia pseudosacchari

<220>

<223> nifA

<400> 143

atgacccagc gaaccgagtc gggtaatacc gtctggcgct tcgatttatc ccagcagttc 60

accgcgatgc agcggataag cgtggttctc agccgggcga ccgaggttga acagacactc 120

cagcaggtgc tgtgcgtatt gcacaatgac gccttttttgc agcacggcat gatctgtctg 180

tacgacagcc agcaggcgat tttgactatt gaagcgttgc aggaagccga tcagcagttg 240

atccccggca gctcgcaaat tcgctaccgt ccgggtgaag ggctggtcgg gacggtgctt 300

tcgcaggggc aatcgttagt gctggcgcgt gtggctgacg atcagcgctt tcttgaccgc 360

ctgggactgt atgattacaa ccgccgttt atcgccgtgc cgctgatagg gccggatgcg 420

cagacttttg gcgtgctgac ggcgcaaccg atggcgcgtt acgaagagcg gttacccgcc 480

tgcacccgct ttctggaaac ggtcgcgaat ctggtggcgc agaccgtgcg tttgatgacg 540

ccgccggctg cacgcccttc cccacgcgct gccatcacgc caaccgccag cccgaaatcg 600

tgcagtactt cacgcgcgtt cggcttcgaa aatatggtcg gcaacagccc ggcaatgcgc 660

cagaccatgg agattatccg tcaggtttcg cgctgggata ccaccgttct ggtgcgcggc 720

gagagcggca ccggcaagga actgattgcc aacgccatcc atcacaattc gccgcgcgcc 780

agtgcgccat ttgtgaaatt caactgtgcg gcgctgccgg acacattgct tgaaagcgaa 840

ttatttggtc atgaaaaagg cgcctttacc ggcgcggtac gccagcgtaa aggccgtttt 900

gagctggccg atggcggcac gctgtttctt gacgaaattg gggaaagcag cgcctcgttt 960

caggctaagc tgctgcgtat tttgcaggag ggcgaaatgg aacgcgtcgg tggtgacgag 1020

acattgcaag tgaatgtgcg catcattgcc gcgacgaacc gcaaccttga agatgaagta 1080

cgcctgggac attttcgcga agatctctat taccgcctga atgtgatgcc catcgccctg 1140

ccgccgctgc gcgaacgcca ggacgacatc gccgaactgg cacattttct ggtgcgtaaa 1200

atcgcccaca accagaaccg cacgctgcgc attagcgagg gcgctatccg cctgctgatg 1260

agctacagct ggcccggcaa tgtgcgcgaa ctggaaaact gccttgagcg ctctgcggtg 1320

atgtcggaaa acggtctgat cgatcgggac gtgattttat ttaatcatcg cgaccagcca 1380

gccaaaccgc cggttatcag cgtcacgccc gacgataact ggctcgataa cacccttgac 1440

gagcgccagc ggctgattgc cgcgctggaa aaagcgggat gggtacaagc caaagccgcc 1500

cgcttgctgg ggatgacgcc gcgccaggtc gcttatcgta ttcagaccat ggatatcacc 1560

ctgccaaggc tataa 1575

<210> 144

<211> 2850

<212> DNA

<213> Kosakonia pseudosacchari

<220>

<223> glnE

<400> 144

atgccgcacc acgcaggatt gtcgcagcac tggcaaacgg ttttttctcg tctgccggaa 60

gcgctcaccg cgcaaccatt gagcgcgcag gcgcagtcag tgctcacttt tagtgatttt 120

gttcaggaca gcatcatcgt gcatcctgag tggctggcag agcttgaaag cgcaccgccg 180

ccagcgaacg agtggcaaca ctacgcgcaa tggctgcaag cggcgctgga gggcgtcacc 240

gatgaaacct cgctgatgcg cacgctgcgg ctgtttcgcc gtcgcattat ggtgcgcatc 300

gcctggagtc aggcgctaca gttggtggcg gaagaggata tcctgcaaca gctcagcgtg 360

ctggcggaaa ctctgatcgt cgccgcgcgc gactggctct atgacgcctg ctgccgtgag 420

tggggaacgc cgtgcaatcc gcaaggcgtc gcgcagccga tgctggtgct cggcatggggc 480

aaacttggcg gcggcgaact caatttctca tccgatatcg atttgatttt tgcctggccg 540

gaaaatggca ccacgcgcgg cggacgccgt gaactggata acgcgcagtt ttttacccgc 600

cttggtcaac ggctaattaa agtcctcgac cagccccgc aggatggctt tgtctaccgc 660

gtcgatatgc gcttgcgtcc ctttggcgac agcggcccgc tggtgctgag ttttgccgcg 720

ctggaagatt actaccagga gcaggggcgc gactgggaac gatacgcgat ggtgaaagcg 780

cgcattatgg gggacaacga cggcgaccat gcgcgagagt tgcgcgccat gctgcgcccg 840

ttcgttttcc gccgctatat cgacttcagc gtgatccagt ctctgcgcaa catgaaaggc 900

atgattgccc gcgaagtgcg gcgtcgcggc ctgaaggaca acataaaact cggcgcgggc 960

ggtattcgcg aaatagagtt tatcgtgcag gttttccagt tgattcgcgg cggtcgcgag 1020

cctgcgctgc aatcgcgttc gctgttgccg acgcttgctg ccattgatca actacatctg 1080

ctgccggatg gtgatgcacc ccggctgcgc gaggcgtatt tgtggctgcg acggctggaa 1140

aacttgctgc aaagcattaa tgacgaacag aacacagacgc tgccggccga tgatttgaat 1200

cgcgcgcgcc tcgcctgggg aatgggcaaa gagagctggg aagcgctctg cgaaacgctg 1260

gaagcgcata tgtcggcggt gcggcagatt ttcaacgatc tgattggcga tgatgaaacg 1320

1380

gaagaggact ctacgcccgt gctggcgcat ctttccgagg acgatcgccg ccgcgtggtg 1440

gcgctgattg ctgattttcg caaagagctg gataaacgca ccattggccc gcgcgggcga 1500

caggtactcg atcacttaat gccgcatctg ctcagcgatg tatgctcgcg tgacgatgcg 1560

ccagtgccgc tgtcgcgtct gacgccgctg ctcaccggta ttattacgcg caccacttac 1620

cttgagctgc tgagtgaatt ccccggtgcg ctgaaacacc tcatttccct gtgcgccgcg 1680

tcgccgatgg tggccagcca actggcgcgc tacccgatcc tgctcgatga actgctcgac 1740

ccgaacacgc tctatcaacc gacggcgatg aacgcctatc gcgatgaact gcgacaatac 1800

ctgttgcgcg tgccggaaga ggatgaagag cagcaactgg aggcgctacg gcagtttaag 1860

caggcgcagt tgttgcgcgt agcggcggcg gatatcgccg gtacgttacc cgtcatgaaa 1920

gtgagcgatc acttaacctg gctggcggaa gcgattatcg atgcggtggt gcagcaagcc 1980

tggaaccaga tggtggcgcg ttacggccag ccgacgcatc tgcacgatcg cgaagggcgc 2040

ggtttcgccg tggtcggtta cggcaaactt ggcggctggg aattaggtta cagctccgat 2100

ctggatctgg tgttcctgca cgactgcccc atggatgtga tgaccgatgg cgagcgtgaa 2160

atcgatggcc gccagttcta tttgcgcctc gcgcagcgcg tgatgcacct gttcagcacg 2220

cgcacgtcgt ccggcattct ttatgaagtc gatgcgcgtt tgcgcccgtc cggcgcggcc 2280

ggaatgctgg tgaccactgc ggaagcgttc gccgattatc aaaaaaatga agcctggaca 2340

tgggagcatc aggcgctggc gcgtgcgcgc gtggtgtacg gcgatccgca actgaccgcc 2400

gaatttgacg ccattcgccg cgatatcctg atgacctccc gcgatgccgc taccctgcaa 2460

accgaagtgc gggaaatgcg tgagaaaatg cgcgcccatc ttggtaacaa gcacaaagac 2520

2580

ctggtgctgc gctttgccca tgagaagccg aaactgacgc gctggtcgga taatgtgcgc 2640

atcctcgaag ggctggcgca aaacggcatc atggatgagc aggaagcgca ggcattgacg 2700

ctggcgtaca ccacgttgcg tgatgagctg caccacctgg cgctgcaaga gctgccagga 2760

catgtggcgc tctcctgttt tgtcgccgag cgtgcgctta tcaaaaccag ctgggacaag 2820

tggctggtgg aaccgtgcgc cccggcgtaa 2850

<210> 145

<211> 1287

<212> DNA

<213> Kosakonia pseudosacchari

<220>

<223> amtB

<400> 145

atgaaaaaca caacattaaa aacggctctt gcttcgctgg cgttgctgcc aggcctggcg 60

atggcggctc ccgctgtggc ggataaagcc gacaacggct ttatgatgat ttgcaccgcg 120

ctggtgctgt ttatgaccat tccgggcatt gcgctgttct acggcggttt gatccgcggt 180

aaaaacgtgc tgtcgatgct gacgcaggtt gccgtcacct tcgctctggt gtgcatcctg 240

tgggtggttt acggctactc tctggcattt ggcgagggca acagcttctt cggcagtttc 300

aactgggcga tgttgaaaaa catcgaattg aaagccgtga tgggcagcat ttatcagtac 360

atccacgtgg cgttccaggg ctcctttgct tgtatcaccg ttggcctgat tgtcggtgcg 420

ctggctgagc gtattcgctt ctctgcggtg ctgatttttg tggtggtatg gctgacgctt 480

tcttatgtgc cgattgcgca catggtctgg ggtggcggtc tgctggcaac ccacggcgcg 540

ctggatttcg cgggcggtac ggttgttcac atcaacgccg cgatcgcagg tctggtgggg 600

gcttacctga ttggcaaacg cgtgggcttt ggcaaagaag cgttcaaacc gcataacctg 660

ccgatggtct tcaccggcac cgcgatcctc tatgttggct ggtttggctt caacgccggc 720

tctgcaagct cggcgaacga aatcgctgcg ctggctttcg tgaacacggt tgttgccact 780

gcggccgcta ttctggcgtg ggtatttggc gagtgggcaa tgcgcggtaa gccgtctctg 840

ctcggtgcct gttctggtgc catcgcgggt ctggttggta tcaccccggc gtgcggttat 900

gtgggtgtcg gcggcgcgct gattgtgggt ctgattgccg gtctggcagg gctgtggggc 960

gttactgcac tgaaacgtat gttgcgtgtt gatgacccat gcgatgtctt cggtgtgcac 1020

ggcgtgtgcg gcatcgtggg ttgtatcctg accggtatct tcgcgtctac gtcgctgggc 1080

ggtgtcggtt tcgctgaagg ggtgaccatg ggccatcagg tactggtaca gctggaaagc 1140

gttgccatca ctatcgtgtg gtctggcgtg gtggccttta tcggttacaa actggcggat 1200

atgacggtag gcctgcgcgt accggaagag caagagcgtg aagggctgga tgtgaacagc 1260

cacggcgaaa atgcgtataa cgcctga 1287

<210> 146

<211> 500

<212> DNA

<213> Kosakonia pseudosacchari

<220>

<223> PinfC

<400> 146

ttcttggttc tctggagcgc tttatcggca tcctgactga agaatttgca ggcttcttcc 60

caacctggct tgcacccgtg caggtagttg tgatgaacat cactgattcg caggctgaat 120

acgttaacga attgacccgt aaactgcaaa atgcgggcat tcgtgtaaaa gcagacttga 180

gaaacgagaa gattggcttt aaaatccgcg agcacacttt acgtcgtgtc ccttatatgc 240

tggtttgtgg tgacaaagag gtcgaagccg gcaaagttgc tgtgcgtacc cgtcgcggta 300

aagacctggg tagcctggac gtaaatgatg ttatcgagaa gctgcaacaa gagattcgca 360

gccgcagtct tcaacaactg gaggaataag gtattaaagg cggaaaacga gttcaaacgg 420

cgcgtcccaa tcgtattaat ggcgagattc gcgccacggga agttcgctta acaggtctgg 480

aaggcgagca gcttggtatt 500

<210> 147

<211> 348

<212> DNA

<213> Kosakonia pseudosacchari

<220>

<223> Prm1

<400> 147

cgttctgtaa taataaccgg acaattcgga ctgattaaaa aagcgccctc gcggcgcttt 60

ttttatattc tcgactccat ttaaaataaa aaatccaatc ggatttcact atttaaactg 120

gccattatct aagatgaatc cgatggaagc tcgctgtttt aacacgcgtt ttttaacctt 180

ttattgaaag tcggtgcttc tttgagcgaa cgatcaaatt taagtggatt cccatcaaaa 240

aaatattctc aacctaaaaa agtttgtgta atacttgtaa cgctacatgg agattaactc 300

aatctagagg gtattaataa tgaatcgtac taaactggta ctgggcgc 348

<210> 148

<211> 339

<212> DNA

<213> Kosakonia pseudosacchari

<220>

<223> Prm7

<400> 148

cgcgtcaggt tgaacgtaaa aaagtcggtc tgcgcaaagc acgtcgtcgt ccgcagttct 60

ccaaacgtta attggtttct gcttcggcag aacgattggc gaaaaaaccc ggtgcgaacc 120

gggttttttt atggataaag atcgtgttat ccacagcaat ccattgatta tctcttcttt 180

ttcagcattt ccagaatccc ctcaccacaa agcccgcaaa atctggtaaa ctatcatcca 240

attttctgcc caaatggctg ggattgttca ttttttgttt gccttacaac gagagtgaca 300

gtacgcgcgg gtagttaact caacatctga ccggtcgat 339

<210> 149

<211> 1538

<212> DNA

<213> Enterobacter species

<220>

<223> 16S

<220>

<221> modified base

<222> (62)..(62)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (265)..(265)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (457)..(457)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (469)..(469)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (539)..(539)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (551)..(552)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (559)..(559)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (561)..(561)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (576)..(576)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (587)..(587)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (591)..(591)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (655)..(657)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (665)..(665)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (678)..(678)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (706)..(706)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (710)..(710)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (714)..(714)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (718)..(718)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (735)..(735)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (755)..(755)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (759)..(759)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (796)..(796)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (1006)..(1007)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (1016)..(1017)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (1133)..(1133)

<223> a, c, t, g, unknown or other

<400> 149

ttgaagagtt tgatcatggc tcagattgaa cgctggcggc aggcctaaca catgcaagtc 60

gngcggaagc acaggagagc ttgctctctg ggtgacgagc ggcggacggg tgagtaatgt 120

ctgggaaact gcctgatgga gggggataac tactggaaac ggtagctaat accgcataac 180

gtcgcaagac caaagagggg gaccttcgggg cctcttgcca tcagatgtgc ccagatggga 240

ttagctagta ggtggggtaa cggcncacct aggcgacgat ccctagctgg tctgagagga 300

tgaccagcca cactggaact gagacacggt ccagactcct acgggaggca gcagtgggga 360

atattgcaca atgggcgcaa gcctgatgca gccatgccgc gtgtatgaag aaggccttcg 420

ggttgtaaag tactttcagc ggggaggaag gtgttgnggt taataaccnc agcaattgac 480

gttacccgca gaagaagcac cggctaactc cgtgccagca gccgcggtaa tacggaggnt 540

gcaagcgtta nncggaatna ntgggcgtaa agcgtncgca ggcggtntgt naagtcggat 600

gtgaaatccc cgggctcaac ctgggaactg cattcgaaac tggcaggcta gagtnnngta 660

gaggngggta gaattccngg tgtagcggtg aaatgcgtag agatcnggan gaanaccngt 720

ggcgaaggcg gcccnctgga caaagactga cgctnaggng cgaaagcgtg gggagcaaac 780

aggattagat accctngtag tccacgccgt aaacgatgtc gacttggagg ttgtgccctt 840

gaggcgtggc ttccggagct aacgcgttaa gtcgaccgcc tggggagtac ggccgcaagg 900

ttaaaactca aatgaattga cggggggcccg cacaagcggt ggagcatgtg gtttaattcg 960

atgcaacgcg aagaacctta cctactcttg acatccagag aacttnncag agatgnnttg 1020

gtgccttcgg gaactctgag acaggtgctg catggctgtc gtcagctcgt gttgtgaaat 1080

1140

gaactcaaag gagactgcca gtgataaact ggaggaaggt ggggatgacg tcaagtcatc 1200

atggccctta cgagtagggc tacacacgtg ctacaatggc gcatacaaag agaagcgacc 1260

tcgcgagagc aagcggacct cataaagtgc gtcgtagtcc ggattggagt ctgcaactcg 1320

actccatgaa gtcggaatcg ctagtaatcg tagatcagaa tgctacggtg aatacgttcc 1380

1440

ttaaccttcg ggagggcgct taccactttg tgattcatga ctggggtgaa gtcgtaacaa 1500

ggtaaccgta ggggaacctg cggttggatc acctcctt 1538

<210> 150

<211> 882

<212> DNA

<213> Enterobacter species

<220>

<223> nifH

<400> 150

atgaccatgc gtcaatgtgc catttacggc aaaggtggta tcggtaaatc cactaccacg 60

caaaacctgg tcgccgcgct ggcggagatg ggcaagaaag taatgatcgt cggctgcgac 120

ccgaaagcag actccactcg tctgatcctg catgcgaaag cgcagaacac cattatggag 180

atggcggctg aagtcggctc cgtggaagac cttgaactgg aagatgtgct gcaaatcggt 240

tacggcgacg tacgctgcgc agaatccggc ggcccggaac caggcgttgg ctgtgctggt 300

cgcggggtaa ttaccgccat caacttcctg gaagaagaag gcgcctatgt tcccgacctc 360

gatttcgtct gttacgacgt gttgggcgac gtggtgtgcg gggggttcgc catgccgatt 420

cgcgaaaaca aagcgcagga gatctacatc gtctgctccg gcgaaatgat ggcgatgtac 480

gccgccaaca acatctctaa aggcatcgtg aaatacgcca aatccggcaa agtgcgcctt 540

ggcgggctga tctgtaactc ccgtcagacc gaccgcgaag atgagctgat catagcgctg 600

gcggaaaaac tcggcaccca gatgatccac ttcgtgccgc gcgacaacat cgtgcaacgc 660

gctgaaatcc gccgtatgac ggtgattgag tacgatccga aatgcaacca ggccaatgaa 720

taccgcacgc tggcgaacaa gatcgtcaac aacaccaaaa tggtcgtgcc aacgcccatc 780

accatggacg aactggaaga gctgttgatg gaattcggca ttatggatgt ggaagacacc 840

agcattatcg gtaaaaccgc cgcagaagaa aacgcggttt ga 882

<210> 151

<211> 1449

<212> DNA

<213> Enterobacter species

<220>

<223> nifD1

<400> 151

atgagcaatg caacaggcga acgtaatctg gagatcatcc aggaagtgct ggagatcttt 60

ccggaaaaaa cgcgcaaaga acgcagaaag cacatgatgg tgagcgaccc ggagatggaa 120

agcgtcggga aatgcatcat ctccaaccgt aagtcgcagc ccggcgtaat gaccgtgcgc 180

ggttgctctt acgccggttc taaaggggtg gtattcgggc cgatcaaaga tatggcccat 240

atttccccg gcccggtcgg ctgcggtcag tactcccgcg ccgggcggcg taactactac 300

accggcgtca gcggtgtgga tagcttcggt acgctcaact ttacctccga ttttcaggag 360

cgcgatatcg tgtttggcgg cgataaaaag ctgaccaaac tgattgaaga gatggagacg 420

ctgttcccgc tgaccaaagg gatctccatt cagtccgaat gcccggtcgg cctgattggc 480

gacgacattg aagccgttgc caacgccagc cgcaaagcca tcaataaacc ggtcattccg 540

gtgcgctgcg aaggttttcg cggcgtttcc cagtcactcg gtcaccacat tgccaacgac 600

gtgatccgcg actgggtact ggataaccgc gaaggcaagc cgtttgaggc cggtccttat 660

720

ctcgaagaga tgggcctgcg cgtggtggcg cagtggtccg gcgacggcac gctggttgag 780

atggagaaca cgccgttcgt caaactcaac cttgtgcact gctaccgctc aatgaactat 840

atctcccgcc atatggagga gaaacacggt attccgtgga tggagtacaa cttcttcggt 900

ccgaccaaag tcgccgaatc gttgcgcaaa atcgccgata tgtttgatga caccattcgc 960

gccaacgccg aagcggtgat cgccaaatat caggcgcaga acgacgccat catcgccaaa 1020

taccgtccgc gtctggaagg ccgcaaagtg ctgctgtata tgggcggttt acgtcctcgc 1080

catgtgattg gcgcttatga agatctgggg atggaaatta tcgctgcggg ttatgaattc 1140

gcccacaacg atgactacga ccgcaccctg ccggatctga aagaaggcac cttgctgttc 1200

gacgatgcca gcagttatga actggaagcc tttgtcaaag cgctgaagcc ggatctgatc 1260

ggctccggca ttaaagagaa gtacatcttc cagaaaatgg gcgtgccgtt tcgccagatg 1320

cactcctggg attactccgg cccctatcac ggttatgacg gctttgccat cttcgcccgc 1380

1440

tccgcctga 1449

<210> 152

<211> 1092

<212> DNA

<213> Enterobacter species

<220>

<223> nifD2

<220>

<221> modified base

<222> (709)..(709)

<223> a, c, t, g, unknown or other

<400> 152

atgggacgcg gcgagcgccg cctgttccat gccgtgcgcc acatcgtcaa ccgctaccac 60

ccggccgccg tctttatcta taacacctgc gttcccgcga tggagggcga cgatatcgaa 120

gccgtctgcc aggcggcaga aaccgccatc ggcgtaccgg tgattgccgt tgatgtcgcc 180

gggttttacg gcagcaaaaa tctcggcaac cggttggccg gtgaagtgat ggtgaaaaag 240

gtgattggcg ggcgtgaacc cgcgccgtgg ccggaagata ccccttttgc cccggcgcac 300

cgccacgata tcgggctgat tggcgaattc aatattgccg gagagttctg gcatattcag 360

ccgctgctcg atgagctggg tattcgcgtg ctcggcagcc tctccggcga cgggcgcttc 420

agtgaaatcc agacgctgca ccgggcgcag gtcaatatgc tggtctgctc cagggcgctg 480

atcaacgtcg cccgctcgct ggagcagcgc tacggcacgc cgtggtttga aggcagtttt 540

tatggtgttc gcgccacctc tgacgccctg cgccaactgg cggcgctgac cggagaccgc 600

gatctgatgc agcgcaccga acagctcatt gcccgcgaag agcagcaaac agagcaggcg 660

ctggccccgc tgcgcgagcg cctgcgcggg cgcaaagcgc tgctctatnc cggcggcgtg 720

aaatcctggt cggtggtttc ggcgcttcag gatctgggca tggaagtggt ggcgaccggc 780

acgcgcaaat ccaccgaaga ggataaacag cgcatccgcg aactgatggg cgccgacgcg 840

ctgatgcttg atgaaggtaa cgccgctcg ctgctggacg tggtttaccg ctacaaggcg 900

gacatgatga tcgccggggg acgcaatatg tacaccgcct acaaagcgcg gctgccgttc 960

ctcgatatca atcaggagcg cgagcacgcc tttgccggct accgcggcat tgtcaccctg 1020

gccgaacagc tctgcctgac catggaaagc ccggtctggc cgcaaaccca ttcccgcgca 1080

ccgtggcaat aa 1092

<210> 153

<211> 846

<212> DNA

<213> Enterobacter species

<220>

<223> nifK

<400> 153

atgatggagc aaatggacgt gccgtgcagc ctgctttccg atccctccga agtgctggat 60

accccggctg acgggcatta ccacatgtat gcgggcggta cgacccagca ggagatgcgc 120

gaagcgcctg acgctatcga caccctgctg ctgcaaccct ggcaactggt gaaaaccaaa 180

aaagtggtgc aggaaagctg gaaccagccc gctaccgagg tgcaaatccc aatggggctg 240

gccggaaccg acgagctgct gatgacggta agccagttaa ccggcaaagc cattccggat 300

agcttagcgc tggaacgcgg tcggctggtg gatatgatgc tcgactccca cacctggctg 360

cacggcaaga aattcggcct gttcggtgac ccggattttg tcatggggct gacccgcttc 420

ctgctggaac tgggctgcga accgacggtg attctgtgcc ataacggcag caagcgctgg 480

cagaaagcga tgaagaaaat gcttgaagcc tcgccgtacg ggaaagagag cgaagtcttt 540

atcaactgcg atttgtggca tttccgctcg ctgatgttta cccgtcagcc ggactttatg 600

atcggcaact cctacgccaa gtttatccag cgcgatacgc tggcgaaggg tgagcagttt 660

gaagtgccgc tgatccgcct ggggttcccg ctgttcgatc gccaccatct gcaccgccag 720

accacctggg gttacgaagg ggccatgagt atcctcacca cgctggttaa tgcggtgctg 780

gagaaagtcg acagagagac catcaagctc ggcaaaaccg actacagctt cgatcttatc 840

cgttaa 846

<210> 154

<211> 1095

<212> DNA

<213> Enterobacter species

<220>

<223> nifL

<220>

<221> modified base

<222> (942)..(942)

<223> a, c, t, g, unknown or other

<400> 154

atgcagcgcg acatcagcac cagctacgcg ctggaacaac ggctgcgcaa tcatatgacg 60

ctgaccgaag ccgtcttgaa taacattccg gcggcggttg tagtggtgga tgaacgcgat 120

180

ctactcaccg aaatcaactt ttccgcccat aaggcggagc tggcgcaggg cctggtactg 240

ccggtagtgc tgcgcggcac cgtgcgctgg ttgtccgtta cctgttggggc gctgccggggc 300

gtcagcgaag aagcaggccg ctactttatt gatagcgccg tgccgcgcac gctggtggtg 360

atcaccgata atactcagca gcagcaacaa caggagcagg ggcgtcttga tcgtctgaag 420

cagcagataa ccagcggtaa attgctggcg gcgatccgcg aatcgctgga cgccgcgctg 480

gtacaactca attgcccaat taatatgctg gccgccgcac gccgcttaaa tggcgacgag 540

catagcaatc tggcgctgga tgccgcatgg cgtgaaggcg aagaagcgat ggcgcggttg 600

cagcgctgcc gcccgtcgct ggaactggaa agcccggcag tctggccgct cgccgttc 660

cttgacgatc tgcgtgccct gtatcacacc cgatataacc agggcgaaaa cctgcaaatt 720

gagctggaat cccccgacct ggtgggcttt ggccagcgaa cacaactgct tgcctgcctg 780

agcctgtggc tcgacagaac cctggatatt gccgcggagc tacgtgattt cacggtacag 840

actcaacttt acgcccgcga agagagcggc tggctgtcgt tctatttaaa cgacaatgtg 900

ccgctgattc aggtgcgcta cacccattca cccgatgcac tnaatgcgcc cggtaaaggc 960

atggagctgc ggctgatcca gacgctggtc gcccaccatc gaggcgcaat agaactgacc 1020

tcacgccctc agggaggcac ctgtctgatc ctgcgtttcc cattatttta ctcgctgaca 1080

ggaggctcac tatga 1095

<210> 155

<211> 219

<212> DNA

<213> Enterobacter species

<220>

<223> partial nifA gene

<400> 155

atgactcagc gaaccgagtc gggtacaacc gtctggcgct ttgacctctc ccaacagttt 60

acagccatgc agcgtatcag tgtggtgtta agccgcgcga cggagatcgg gcagacgcta 120

caggaagtgc tgtgcgtgct gcacaacgat gcctttatgc agcacgggat gatctgtccg 180

tacgcgcggg tgcgcgtctt cgcgagcgta tggctttga 219

<210> 156

<211> 1635

<212> DNA

<213> Enterobacter species

<220>

<223> glnE

<220>

<221> modified base

<222> (234)..(234)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (274)..(274)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (1535)..(1535)

<223> a, c, t, g, unknown or other

<400> 156

atgcgcgtgg aagactggtc aacgctgacc gaacggctcg atgcccatat ggcaggcgtg 60

cgccgaatct ttaacgaact gatcggtgat gacgaaagtg agtcgcagga cgatgcgctc 120

tccgagcact ggcgcgagct gtggcaggac gcgcttcagg aagatgacac cacgccggtg 180

ctgacgcact taaccgacga cgcgcgccat cgcgtggtgg cgctgatcgc tganttccgt 240

cttgagctga acaaacgcgc catcggcccg cgtngtcgcc aggtgctgga tcacctgatg 300

ccgcacctgc tgagcgaagt ctgctcgcgt gccgatgcgc cggtgccgct gtcgcggatg 360

atgcccctgc tgagcgggat tatcacccgt actacctacc ttgaactcct gagcgagttc 420

cctggcgcgc ttaagcacct gatttcactc tgcgccgcgt cgccgatggt ggccaacaag 480

ctggcgcgtt acccgctgct gctggatgag ctgctcgatc cgaataccct ttatcaaccg 540

acggcgaccg acgcctaccg ggacgaactg cgtcagtatc tgctgcgcgt gccggaagaa 600

gacgaagagc aacagctgga ggcgctgcgt cagtttaagc aggcccagat gctgcgcgtg 660

gcggccgcag atattgccgg aacgctgccg gtgatgaaag tgagcgatca cttaacctgg 720

cttgcggaag cgattatcga cgcggtggtg catcaggcct gggtgcagat ggtggcgcgc 780

tatggccagc cgaaacatct ggctgaccgt gatggtcgcg gcttcgcggt ggtgggttac 840

ggtaagctcg gcggttggga gctgggctat agctccgatc tggatttaat cttcctccac 900

gactgcccgg ttgatgtgat gaccgacggc gagcgcgaga ttgacgggcg tcagttctac 960

ctgcgcctgg cgcagcgcat catgcacctg ttcagcaccc gcacctcgtc gggcattttg 1020

tatgaagtgg atgcccgtct gcgcccgtcc ggcgcggcgg gcatgctggt cacctcgacg 1080

gagtccttcg ctgattacca gaagaatgaa gcctggacgt gggagcatca ggcgctggtg 1140

cgcgcccgtg tggtgtatgg cgatccgctg ctgaaaacgc agtttgacgt gattcgtaag 1200

gaagtcatga ccaccgtgcg cgatggcagc acgctgcaaa cggaagtgcg cgaaatgcgc 1260

gagaaaatgc gcgcgcactt aggcaataaa catcgcgatc gctttgatat taaagccgat 1320

gagggcggta ttaccgatat tgagtttatt acccagtatc tggtgttgct gcacgcgcat 1380

gacaagccga agctgacgcg ctggtcggat aacgtgcgca ttctggaact gctggcgcaa 1440

aacgacatta tggacgagca ggaggcgcag gccttaaccc gtgcctatac aacgcttcgc 1500

gatgagctcc atcatctggc gttgcaggag cagcngggac acgtggcgct ggactgtttc 1560

accgctgaac gcgctcaggt aacggccagc tggcagaagt ggctggtgga accgtgcgta 1620

acaaatcaag tgtga 1635

<210> 157

<211> 1316

<212> DNA

<213> Enterobacter species

<220>

<223> 16S

<220>

<221> modified base

<222> (43)..(43)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (247)..(247)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (784)..(785)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (794)..(795)

<223> a, c, t, g, unknown or other

<400> 157

agatgtgccc agatgggatt agctagtagg tggggtaacg gcncacctag gcgacgatcc 60

ctagctggtc tgagaggatg accagccaca ctggaactga gacacggtcc agactcctac 120

gggaggcagc agtggggaat attgcacaat gggcgcaagc ctgatgcagc catgccgcgt 180

gtatgaagaa ggccttcggg ttgtaaagta ctttcagcgg ggaggaaggt gttgtggtta 240

ataaccncag caattgacgt tacccgcaga agaagcaccg gctaactccg tgccagcagc 300

cgcggtaata cggagggtgc aagcgttaat cggaattact gggcgtaaag cgcacgcagg 360

cggtctgtca agtcggatgt gaaatccccg ggctcaacct gggaactgca ttcgaaactg 420

gcaggctaga gtcttgtaga ggggggtaga attccaggtg tagcggtgaa atgcgtagag 480

atctggagga ataccggtgg cgaaggcggc cccctggaca aagactgacg ctcaggtgcg 540

aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa acgatgtcga 600

cttggaggtt gtgcccttga ggcgtggctt ccggagctaa cgcgttaagt cgaccgcctg 660

gggagtacgg ccgcaaggtt aaaactcaaa tgaattgacg ggggcccgca caagcggtgg 720

agcatgtggt ttaattcgat gcaacgcgaa gaaccttacc tactcttgac atccagagaa 780

cttnncagag atgnnttggt gccttcggga actctgagac aggtgctgca tggctgtcgt 840

cagctcgtgt tgtgaaatgt tgggttaagt cccgcaacga gcgcaaccct tatcctttgt 900

tgccagcggt ccggccggga actcaaagga gactgccagt gataaactgg aggaaggtgg 960

ggatgacgtc aagtcatcat ggcccttacg agtagggcta cacacgtgct acaatggcgc 1020

atacaaagag aagcgacctc gcgagagcaa gcggacctca taaagtgcgt cgtagtccgg 1080

attggagtct gcaactcgac tccatgaagt cggaatcgct agtaatcgta gatcagaatg 1140

ctacggtgaa tacgttcccg ggccttgtac acaccgcccg tcacaccatg ggagtgggtt 1200

gcaaaagaag taggtagctt aaccttcggg agggcgctta ccactttgtg attcatgact 1260

ggggtgaagt cgtaacaagg taaccgtagg ggaacctgcg gttggatcac ctcctt 1316

<210> 158

<211> 882

<212> DNA

<213> Enterobacter species

<220>

<223> nifH

<400> 158

atgaccatgc gtcaatgtgc catttacggc aaaggtggta tcggtaaatc cactaccacg 60

caaaacctgg tcgccgcgct ggcggagatg ggcaagaaag taatgatcgt cggctgcgac 120

ccgaaagcag actccactcg tctgatcctg catgcgaaag cgcagaacac cattatggag 180

atggcggctg aagtcggctc cgtggaagac cttgaactgg aagatgtgct gcaaatcggt 240

tacggcgacg tacgctgcgc agaatccggc ggcccggaac caggcgttgg ctgtgctggt 300

cgcggggtaa ttaccgccat caacttcctg gaagaagaag gcgcctatgt tcccgacctc 360

gatttcgtct gttacgacgt gttgggcgac gtggtgtgcg gggggttcgc catgccgatt 420

cgcgaaaaca aagcgcagga gatctacatc gtctgctccg gcgaaatgat ggcgatgtac 480

gccgccaaca acatctctaa aggcatcgtg aaatacgcca aatccggcaa agtgcgcctt 540

ggcgggctga tctgtaactc ccgtcagacc gaccgcgaag atgagctgat catagcgctg 600

gcggaaaaac tcggcaccca gatgatccac ttcgtgccgc gcgacaacat cgtgcaacgc 660

gctgaaatcc gccgtatgac ggtgattgag tacgatccga aatgcaacca ggccaatgaa 720

taccgcacgc tggcgaacaa gatcgtcaac aacaccaaaa tggtcgtgcc aacgcccatc 780

accatggacg aactggaaga gctgttgatg gaattcggca ttatggatgt ggaagacacc 840

agcattatcg gtaaaaccgc cgcagaagaa aacgcggttt ga 882

<210> 159

<211> 1449

<212> DNA

<213> Enterobacter species

<220>

<223> nifD1

<400> 159

atgagcaatg caacaggcga acgtaatctg gagatcatcc aggaagtgct ggagatcttt 60

ccggaaaaaa cgcgcaaaga acgcagaaag cacatgatgg tgagcgaccc ggagatggaa 120

agcgtcggga aatgcatcat ctccaaccgt aagtcgcagc ccggcgtaat gaccgtgcgc 180

ggttgctctt acgccggttc taaaggggtg gtattcgggc cgatcaaaga tatggcccat 240

atttccccg gcccggtcgg ctgcggtcag tactcccgcg ccgggcggcg taactactac 300

accggcgtca gcggtgtgga tagcttcggt acgctcaact ttacctccga ttttcaggag 360

cgcgatatcg tgtttggcgg cgataaaaag ctgaccaaac tgattgaaga gatggagacg 420

ctgttcccgc tgaccaaagg gatctccatt cagtccgaat gcccggtcgg cctgattggc 480

gacgacattg aagccgttgc caacgccagc cgcaaagcca tcaataaacc ggtcattccg 540

gtgcgctgcg aaggttttcg cggcgtttcc cagtcactcg gtcaccacat tgccaacgac 600

gtgatccgcg actgggtact ggataaccgc gaaggcaagc cgtttgaggc cggtccttat 660

720

ctcgaagaga tgggcctgcg cgtggtggcg cagtggtccg gcgacggcac gctggttgag 780

atggagaaca cgccgttcgt caaactcaac cttgtgcact gctaccgctc aatgaactat 840

atctcccgcc atatggagga gaaacacggt attccgtgga tggagtacaa cttcttcggt 900

ccgaccaaag tcgccgaatc gttgcgcaaa atcgccgata tgtttgatga caccattcgc 960

gccaacgccg aagcggtgat cgccaaatat caggcgcaga acgacgccat catcgccaaa 1020

taccgtccgc gtctggaagg ccgcaaagtg ctgctgtata tgggcggttt acgtcctcgc 1080

catgtgattg gcgcttatga agatctgggg atggaaatta tcgctgcggg ttatgaattc 1140

gcccacaacg atgactacga ccgcaccctg ccggatctga aagaaggcac cttgctgttc 1200

gacgatgcca gcagttatga actggaagcc tttgtcaaag cgctgaagcc ggatctgatc 1260

ggctccggca ttaaagagaa gtacatcttc cagaaaatgg gcgtgccgtt tcgccagatg 1320

cactcctggg attactccgg cccctatcac ggttatgacg gctttgccat cttcgcccgc 1380

1440

tccgcctga 1449

<210> 160

<211> 1374

<212> DNA

<213> Enterobacter species

<220>

<223> nifD2

<400> 160

atgaagggga acgagatcct ggctttgctc gatgaacctg cctgcgagca caaccataaa 60

cagaaatccg gctgcagcgc gccgaaaccc ggcgcgacag cgggcggctg cgcctttgac 120

ggtgcgcaga tcaccctgct gccactctcc gatgttgccc acctggtaca cggccccatt 180

ggttgtaccg gtagctcatg ggataaccgt ggcagcttca gttccggccc gacgatcaac 240

cggctgggtt ttaccaccga tctgagcgaa caggatgtga tcatgggacg cggcgagcgc 300

cgcctgttcc atgccgtgcg ccacatcgtc aaccgctacc acccggccgc cgtctttatc 360

tataacacct gcgttcccgc gatggagggc gacgatatcg aagccgtctg ccaggcggca 420

gaaaccgcca tcggcgtacc ggtgattgcc gttgatgtcg cggggtttta cggcagcaaa 480

aatctcggca accggttggc cggtgaagtg atggtgaaaa aggtgattgg cgggcgtgaa 540

600

attggcgaat tcaatattgc cggagagttc tggcatattc agccgctgct cgatgagctg 660

ggtattcgcg tgctcggcag cctctccggc gacgggcgct tcagtgaaat ccagacgctg 720

caccgggcgc aggtcaatat gctggtctgc tccagggcgc tgatcaacgt cgcccgctcg 780

ctggagcagc gctacggcac gccgtggttt gaaggcagtt tttatggtgt tcgcgccacc 840

tctgacgccc tgcgccaact ggcggcgctg accggagacc gcgatctgat gcagcgcacc 900

gaacagctca ttgcccgcga agagcagcaa acagagcagg cgctggcccc gctgcgcgag 960

cgcctgcgcg ggcgcaaagc gctgctctat accggcggcg tgaaatcctg gtcggtggtt 1020

tcggcgcttc aggatctggg catggaagtg gtggcgaccg gcacgcgcaa atccaccgaa 1080

gaggataaac agcgcatccg cgaactgatg ggcgccgacg cgctgatgct tgatgaaggt 1140

aacgcccgct cgctgctgga cgtggtttac cgctacaagg cggacatgat gatcgccggg 1200

ggacgcaata tgtacaccgc ctacaaagcg cggctgccgt tcctcgatat caatcaggag 1260

cgcgagcacg cctttgccgg ctaccgcggc attgtcaccc tggccgaaca gctctgcctg 1320

accatggaaa gcccggtctg gccgcaaacc cattcccgcg caccgtggca ataa 1374

<210> 161

<211> 1563

<212> DNA

<213> Enterobacter species

<220>

<223> nifK1

<400> 161

atgagccaaa gtgctgagaa aattcaaaac tgtcatccgc tgtttgaaca ggatgcgtac 60

cagatgctgt ttaaagataa acggcaactg gaagaggccc acgatccggc gcgcgtgcag 120

gaggtctttc aatggaccac caccgccgag tatgaagcgc ttaactttca acgcgaagcg 180

ctgactatcg atccggccaa agcctgccag ccgctgggtg cggtactgtg ctcgctgggc 240

tttgccaata ccctgcccta tgttcacggc tcccaggggt gcgtggccta tttccgcacc 300

tattttaacc gtcactttaa agagccgatt gcctgtgttt ctgactcgat gacggaagat 360

gcggcagtat tcggcggcaa caacaacctg aacaccgggt tgcagaacgc cagcgccctc 420

tacaagccgg aaatcattgc cgtctccacc acctgtatgg cggaggtcat cggcgacgac 480

ctgcaggcgt ttattgctaa cgccaaaaaa gacggcttta tcgacgcggc gatcccggtg 540

600

gagggcttcg cccgtacctt taccgccgat tacagcggac aaccgggcaa attaccgcgt 660

atcaatctgg tcagcggatt tgaaacctat ctcggtaatt tccgcgtgct gaaacgcatg 720

atggagcaaa tggacgtgcc gtgcagcctg ctttccgatc cctccgaagt gctggatacc 780

ccggctgacg ggcattacca catgtatgcg ggcggtacga cccagcagga gatgcgcgaa 840

gcgcctgacg ctatcgacac cctgctgctg caaccctggc aactggtgaa aaccaaaaaa 900

gtggtgcagg aaagctggaa ccagcccgct accgaggtgc aaatcccaat ggggctggcc 960

ggaaccgacg agctgctgat gacggtaagc cagttaaccg gcaaagccat tccggatagc 1020

ttagcgctgg aacgcggtcg gctggtggat atgatgctcg actcccacac ctggctgcac 1080

ggcaagaaat tcggcctgtt cggtgacccg gattttgtca tggggctgac ccgcttcctg 1140

ctggaactgg gctgcgaacc gacggtgatt ctgtgccata acggcagcaa gcgctggcag 1200

1260

aactgcgatt tgtggcattt ccgctcgctg atgtttaccc gtcagccgga ctttatgatc 1320

ggcaactcct acgccaagtt tatccagcgc gatacgctgg cgaagggtga gcagtttgaa 1380

gtgccgctga tccgcctggg gttcccgctg ttcgatcgcc accatctgca ccgccagacc 1440

acctggggtt acgaaggggc catgagtatc ctcaccacgc tggttaatgc ggtgctggag 1500

1560

taa 1563

<210> 162

<211> 1386

<212> DNA

<213> Enterobacter species

<220>

<223> nifK2

<400> 162

atggcagaaa ttatccgtag taaaaagccg ctggccgtca gcccggtaaa aagtggccag 60

ccgctgggcg cgattctggc gagcatggggc tttgaacaga gcattccgct ggttcatggc 120

gctcaggggt gcagcgcctt cgcgaaggtc ttttttatcc agcattttca cgatccgatc 180

ccgctgcaat cgacggcaat ggacccgaca tcgaccatta tgggtgccga tgagaacatc 240

tttaccgcgc tgaatgtgct gtgttcacgc aacaacccga aagcgattgt tctgctgagc 300

actggccttt ccgaggcgca gggcagcgat atttcgcgcg tggtgcgcca gttccgcgat 360

gaatatccgc gccataaagg ggtggcgctg ctgaccgtca acacgccggga ttttacggc 420

agcctggaaa acggctacag cgcggtgctg gagagcatgg ttgaacagtg ggtgccggaa 480

aaaccgcagc cgggcgtgcg caatcgccgc gtgaacctgc tgctcagcca tttgcttacg 540

ccgggcgaca ttgagctgct gcgaagttat gtcgaggcat ttggcctgca gccggtgatg 600

gtgccggatc tttcccagtc gctggatggc catctcgcca gcggggattt ctcgccaatt 660

acccagggcg gcagcagcct gcggctgatt gaacagatgg gacagagtct tggcacgttc 720

gccattggcg tatccctctc ccgcgccgcg caattgctgg cgcagcgcag ccatgcggaa 780

gtggtcaccc tgccgcatct gatgaccatg agccagtgcg atacgtttat tcatcaactg 840

aagcgcctct ccgggcgcga tgttccggcg tggatcgaac gccagcgcgg gcaactgcag 900

gatgcgatga tcgattgtca tatgtggttg cagggcgcgc ctgtcgcgct ggccgccgag 960

ggcgatctgc tcgccgcctg gtgcgatttc gcctgcgata tgggcatggt gcccggcccg 1020

gtggtggcgc cggtgagcca gaaagggttg caggatctgc cggtcgaaaa agtcattatc 1080

ggcgatctgg aggatatgca ggatctgttg tgtgaaacgc ctgcatcgct gctcgtctct 1140

aattctcacg ccgctgattt ggccgggcag ttcgacattc cgctggtgcg cgccggtttc 1200

cccctgttcg accgtctggg cgagtttcgc cgcgtgcgcc agggttacgc cgggatgcgc 1260

gacaccttgt ttgagctggc gaatgcgctg cgcgatcgcc atcatcatct tgccgcttat 1320

cactcgccgc tgcgccagcg tttttacgaa cccgcatctt cgggaggtga ctatgcaaca 1380

tgttaa 1386

<210> 163

<211> 1488

<212> DNA

<213> Enterobacter species

<220>

<223> nifL

<400> 163

atgaccctga atatgatgat ggacgccacc gcgcccgccg agatcgccgg agcgctctca 60

caacagcatc ccgggttgtt tttcaccatg gttgaacagg cgcccgtcgc gatttcactg 120

accgatgccg atgcccacat tctctacgcc aaccccgcgt tttgtcgcca gtcggggtat 180

gaactggaag agttgttgca gcaaaacccg cgcctgcttg ccagtaagca gacgccgcgt 240

gaaatctacc aggaaatgtg gcacaccctg ctgcaacacc gtccgtggcg cggacaactg 300

atcaaccgtc gccgcgacgg cagcctgttt ctggtggaaa tcgacatcac cccactgttt 360

gatgcgttcg gcaaactcga acattacctg gccatgcagc gcgacatcag caccagctac 420

gcgctggaac aacggctgcg caatcatatg acgctgaccg aagccgtctt gaataacatt 480

ccggcggcgg ttgtagtggt ggatgaacgc gatcgggtgg tgatggataa cctcgcctac 540

aaaacctttt gcgccgattg cggcggtaaa gaactactca ccgaaatcaa cttttccgcc 600

cataaggcgg agctggcgca gggcctggta ctgccggtag tgctgcgcgg caccgtgcgc 660

tggttgtccg ttacctgttg ggcgctgccg ggcgtcagcg aagaagcagg ccgctacttt 720

attgatagcg ccgtgccgcg cacgctggtg gtgatcaccg ataatactca gcagcagcaa 780

caacaggagc aggggcgtct tgatcgtctg aagcagcaga taaccagcgg taaattgctg 840

gcggcgatcc gcgaatcgct ggacgccgcg ctggtacaac tcaattgccc aattaatatg 900

ctggccgccg cacgccgctt aaatggcgac gagcatagca atctggcgct ggatgccgca 960

tggcgtgaag gcgaagaagc gatggcgcgg ttgcagcgct gccgcccgtc gctggaactg 1020

gaaagcccgg cagtctggcc gctccagccg ttccttgacg atctgcgtgc cctgtatcac 1080

acccgatata accagggcga aaacctgcaa attgagctgg aatcccccga cctggtgggc 1140

tttggccagc gaacacaact gcttgcctgc ctgagcctgt ggctcgacag aaccctggat 1200

1260

ggctggctgt cgttctattt aaacgacaat gtgccgctga ttcaggtgcg ctacacccat 1320

tcacccgatg cactcaatgc gcccggtaaa

gtcgcccacc atcgaggcgc aatagaactg acctcacgcc ctcagggagg cacctgtctg 1440

atcctgcgtt tcccattatt ttactcgctg acagggaggct cactatga 1488

<210> 164

<211> 1575

<212> DNA

<213> Enterobacter species

<220>

<223> nifA

<400> 164

atgactcagc gaaccgagtc gggtacaacc gtctggcgct ttgacctctc ccaacagttt 60

acagccatgc agcgtatcag tgtggtgtta agccgcgcga cggagatcgg gcagacgcta 120

caggaagtgc tgtgcgtgct gcacaacgat gcctttatgc agcacgggat gatctgtctg 180

tacgacagta agcaagcgat cctttccatt gaagccttgc atgaggccga tcagcagtta 240

attcccggca gttcacagat tcgctaccgt ccgggcgaag ggctggtagg cacggtgctt 300

tcacagggac agtcgctggt actgccctgt gtctccgacg atcggcgttt tctcgatcgc 360

ctgggattgt atgattacag cttgccgttt atcgccgtgc cgctgatggg gccaaactcg 420

cagcctatcg gcgtgctggc cgcccagcct atggcgcgtt acgaggagcg gctgcccgcc 480

tgcacgcgtt ttcttgaaac cgtcgccaat ctggtggcgc aaaccgttcg cctgatgaca 540

ccgcccagcg tcgcgtctcc accccgtgct gctgccgcgc agattgccag ccagcgcggg 600

tgcgcgtctt cgcgagcgta tggctttgaa aacatggtcg gtaaaagcgc ggctatgcgt 660

cagacgctgg aaattattcg ccaggtatca cgctgggaca ccaccgtgct ggtgcgtggc 720

gaaagcggaa ccggtaaaga gttgatagcc aacgctatcc accacaattc accgcgcgcc 780

gccgcgccgt ttgtcaaatt caactgcgcg gcgctgcccg atacgctgct ggagagtgaa 840

ctcttcggtc atgaaaaagg cgcgtttacc ggcgcggtgc gccagcgcaa aggccgtttc 900

gaactggcgg atggcggtac gctgtttctt gatgagatcg gcgaaagtag cgcctcgttt 960

caggcgaaat tgctgcgtat cttgcaggaa ggcgaaatgg aacgcgtcgg cggcgacgaa 1020

acgctgcggg tgaatgtacg gatcattgcc gccaccaacc gcaatctgga agaggaagtg 1080

cggctgggta attttcgcga agatctctac tatcgcctta atgtgatgcc gatctccctg 1140

cccccgctcc gcgagcgtca ggaggacatc gtcgagctgg cgcattttct ggtgcgcaaa 1200

atcgcgcaaa accagaaccg cacgctgcgc atcagcgatg gcgcgatccg tttgttgatg 1260

agctatagct ggcctggaaa cgtgcgtgag ctggaaaact gccttgagcg atcggcggtg 1320

atgtcggaaa acgggctgat cgatcgcgac gtgattttgt ttcaccacag ggaaaatctg 1380

ccaaaaacgc cacagaccag tgcgccgcgc gaagagagct ggctcgatca gaacctcgat 1440

gagcgacaaa gattgatcgc cgcgctggag aaagccggtt gggtacaggc aaaagccgcg 1500

cgcctgctgg gaatgacccc gcgccaggtg gcctatcgta ttcagacgat ggacattgcc 1560

atgccgagat tgtag 1575

<210> 165

<211> 2856

<212> DNA

<213> Enterobacter species

<220>

<223> glnE

<400> 165

atgccgcttt cttcgcagtt acagcagcag tggcagaccg tttgcgaacg tctgcctgag 60

tcattaccgg cgtcatcgtt aagcgagcag gcaaagagcg tgctcgtctt cagtgatttt 120

gtgcaggaaa gtatcaccgc caacccgaac tggctggcgg aacttgagaa cgcaccaccg 180

caggcagaag agtggcggca ctatgctggc tggctgcaaa ctgtactcga agacgttacg 240

gatgaggcca cgctgatgcg cgtcctgcgc cagttccgtc gtcgggtgat ggtgcgcatt 300

gcctgggctc aggcgctgga actggtgagc gaagagagta cgctgcagca gttaagcgag 360

ctggcgcaaa cgttgattgt cgccgcgcga gactggctct atgccgcctg ctgtaaagag 420

tggggcacgc cgtgcagcga ggaaggggtt cctcagccgc tgttgattct ggggatggga 480

aagctgggcg gctgcgagct gaacttctcc tctgatatcg acctgatttt tgcctggccg 540

gagaacggct ccacgcgcgg aggccgccgc gagctggaca acgcgcagtt ctttacccgt 600

ctcggccagc gcctgattaa agcgctggat cagcccacgc aggacggttt tgtttaccgc 660

gtggacatgc gcctgcgtcc gtttggcgac agcgggccgc tggtgctgag ctttgcggcg 720

ctggaagatt attaccagga gcaaggtcgc gactgggagc gttacgcgat ggtcaaagcg 780

cggatcatgg gcgacagcga cgacgcttat gccaacgagc tgcgcgccat gctgcgtccg 840

ttcgtgttcc gtcgctatat cgacttcagc gtcatccagt ccctgcgaaa tatgaaaggg 900

atgattgccc gcgaggtgcg ccgccgtggg ctgaaagaca atatcaagct cggtgcgggc 960

ggcatccgcg aaatcgaatt tatcgtccag gtcttccagc ttattcgcgg cggacgcgag 1020

ccgtcgctgc agtcccgttc cttattaccg acgctgagcg ccattgcgca gctgcatctc 1080

ctgccggacg gcgacgcgca aaccctgcgc gaggcctatc ttttcctgcg tcgtctggaa 1140

aacctgctgc aaagcattaa tgacgaacag acccaaaccc tgccggggcga cgaccttaac 1200

cgggcgcgtc tggcctgggg aatgcgcgtg gaagactggt caacgctgac cgaacggctc 1260

1320 gatgcccata tggcaggcgt gcgccgaatc tttaacgaac

gagtcgcagg acgatgcgct ctccgagcac tggcgcgagc tgtggcagga cgcgcttcag 1380

1440

gcgctgatcg ctgatttccg tcttgagctg aacaaacgcg ccatcggccc gcgtggtcgc 1500

caggtgctgg atcacctgat gccgcacctg ctgagcgaag tctgctcgcg tgccgatgcg 1560

ccggtgccgc tgtcgcggat gatgcccctg ctgagcggga ttatcacccg tactacctac 1620

1680 cttgaactcc tgagcgagtt ccctggcgcg

tcgccgatgg tggccaacaa gctggcgcgt tacccgctgc tgctggatga gctgctcgat 1740

ccgaataccc tttatcaacc gacggcgacc gacgcctacc gggacgaact gcgtcagtat 1800

ctgctgcgcg tgccggaaga agacgaagag caacagctgg aggcgctgcg tcagtttaag 1860

caggcccaga tgctgcgcgt ggcggccgca gatattgccg gaacgctgcc ggtgatgaaa 1920

gtgagcgatc acttaacctg gcttgcggaa gcgattatcg acgcggtggt gcatcaggcc 1980

tgggtgcaga tggtggcgcg ctatggccag ccgaaacatc tggctgaccg tgatggtcgc 2040

ggcttcgcgg tggtgggtta cggtaagctc ggcggttggg agctgggcta tagctccgat 2100

ctggatttaa tcttcctcca cgactgcccg gttgatgtga tgaccgacgg cgagcgcgag 2160

attgacgggc gtcagttcta cctgcgcctg gcgcagcgca tcatgcacct gttcagcacc 2220

cgcacctcgt cgggcatttt gtatgaagtg gatgcccgtc tgcgcccgtc cggcgcggcg 2280

ggcatgctgg tcacctcgac ggagtccttc gctgattacc agaagaatga agcctggacg 2340

tgggagcatc aggcgctggt gcgcgcccgt gtggtgtatg gcgatccgct gctgaaaacg 2400

cagtttgacg tgattcgtaa ggaagtcatg accaccgtgc gcgatggcag cacgctgcaa 2460

acggaagtgc gcgaaatgcg cgagaaaatg cgcgcgcact taggcaataa acatcgcgat 2520

2580

ctggtgttgc tgcacgcgca tgacaagccg aagctgacgc gctggtcgga taacgtgcgc 2640

attctggaac tgctggcgca aaacgacatt atggacgagc aggaggcgca ggccttaacc 2700

cgtgcctata caacgcttcg cgatgagctc catcatctgg cgttgcagga gcagccggga 2760

cacgtggcgc tggactgttt caccgctgaa cgcgctcagg taacggccag ctggcagaag 2820

tggctggtgg aaccgtgcgt aacaaatcaa gtgtga 2856

<210> 166

<211> 1290

<212> DNA

<213> Enterobacter species

<220>

<223> amtB

<400> 166

atgaagatag caacacttaa aacgggtctg ggttcgctgg cactgctgcc gggcctggcg 60

ctggctgctg cacctgcggt ggcagacaaa gccgataacg cctttatgat gatcagcacc 120

gcgctggtgc tgttcatgtc cattccgggc attgcgctgt tctatggcgg cctgatccgt 180

ggcaaaaacg ttctctccat gctgacgcag gttgccgtaa cgttcgcgct ggtctgcgta 240

ctgtgggtgg tttacggtta ctcgctggct ttcggcacgg gcaacgcgtt ctttggtaac 300

360

tatattcacg ttgctttcca gggctcgttc gcctgcatta ccgtcgggct gattgtaggc 420

gcgcttgccg agcgtattcg tttctctgcg gtcctgatct tcgtggtggt ctggctgacg 480

ctctcctatg tgccgattgc gcacatggtc tggggtggcg gtctgctggc gacgcatggc 540

gcgctggact tcgcgggcgg taccgttgtg cacattaacg ccgcggtagc gggtctggtt 600

ggcgcatacc tgattggcaa acgcgtggggc ttcggtaaag aagcgttcaa accgcacaac 660

ctgccgatgg tcttcaccgg taccgcgatc ctctactttg gctggtttgg tttcaacgcc 720

ggctcagcaa gtgccgcgaa cgaaatcgcc gcgctggcct tcgtgaatac cgttgtggcc 780

acggcaggtg caatcctctc ctgggtcttt ggcgagtggg ctgtgcgcgg taaaccttct 840

ctgctgggtg cctgttcggg ggcgattgct ggtctggtcg gtatcacccc agcatgtggt 900

tatgtcggtg tgggtggcgc gctgctggtc ggcctggtgt caggtctggc gggtctgtgg 960

ggcgtgacgg cgctgaaacg tattctgcgc gttgatgacc cttgcgatgt gtttggcgtg 1020

cacggcgtgt gcggcatcgt cggctgtatc atgaccggta tctttgcagc gaaatcgctg 1080

ggtggcgtgg gctacgcaga aggcgtcacc atggcccatc aggtgctggt gcagctggaa 1140

agtattgctg tcaccgtggt gtggtctgcc gttgtcgctt tcattggcta caaactggcg 1200

gacatgacgg ttggtctgcg cgtgccggaa gagcaggaac gcgaaggtct ggacgtcaac 1260

agccacggcg agaatgcgta taacgcatga 1290

<210> 167

<211> 1299

<212> DNA

<213> Azospirillum lipoferum

<220>

<223> 16S

<220>

<221> modified base

<222> (294)..(294)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (299)..(299)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (302)..(302)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (309)..(310)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (332)..(332)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (346)..(346)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (351)..(351)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (438)..(438)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (445)..(445)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (454)..(454)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (469)..(469)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (477)..(477)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (492)..(492)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (504)..(504)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (555)..(555)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (775)..(775)

<223> a, c, t, g, unknown or other

<400> 167

gccgagagag gggcccgcgt cggattaggt agttggtgag gtaatggctc accaagcctt 60

cgatccgtag ctggtctgag aggatgatca gccacactgg gactgagaca cggcccagac 120

tcctacggga ggcagcagtg gggaatattg gacaatggggc gcaagcctga tccagcaatg 180

ccgcgtgagt gatgaaggcc ttagggttgt aaagctcttt cgcacgcgac gatgatgacg 240

gtagcgtgag aagaagcccc ggctaacttc gtgccagcag ccgcggtaat acgnagggng 300

cnagcgttnn tcggaattac tgggcgtaaa gngcgcgtag gcggcntgtt nagtcagaag 360

tgaaagcccc gggctcaacc tgggaatagc ttttgatact ggcaggcttg agttccggag 420

aggatggtgg aattcccngt gtagnggtga aatncgtaga tattgggang aacaccngtg 480

gcgaaggcgg cnatctggac gganactgac gctgaggcgc gaaagcgtgg ggagcaaaca 540

ggattagata ccctngtagt ccacgccgta aacgatgaat gctagacgtc ggggtgcatg 600

cacttcggtg tcgccgctaa cgcattaagc attccgcctg gggagtacgg ccgcaaggtt 660

aaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa 720

gcaacgcgca gaaccttacc aacccttgac atgtccactt tgggctcgag agatngggtc 780

cttcagttcg gctgggtgga acacaggtgc tgcatggctg tcgtcagctc gtgtcgtgag 840

atgttgggtt aagtcccgca acgagcgcaa cccctaccgt cagttgccat cattcagttg 900

ggcactctgg tggaaccgcc ggtgacaagc cggaggaagg cggggatgac gtcaagtcct 960

catggccctt atgggttggg ctacacacgt gctacaatgg cggtgacagt gggaagcgaa 1020

gtcgcgagat ggagcaaatc cccaaaagcc gtctcagttc ggatcgcact ctgcaactcg 1080

agtgcgtgaa gttggaatcg ctagtaatcg cggatcagca cgccgcggtg aatacgttcc 1140

cgggccttgt acacaccgcc cgtcacacca tgggagttgg ttttacccga aggtggtgcg 1200

ctaaccgcaa ggaggcagcc aaccacggta aggtcagcga ctggggtgaa gtcgtaacaa 1260

ggtagccgta ggggaacctg cggctggatc acctccttt 1299

<210> 168

<211> 897

<212> DNA

<213> Azospirillum lipoferum

<220>

<223> nifH

<400> 168

atggccaaag cgcctctgcg tcagatcgcc ttttacggca agggcggtat cggcaagtcc 60

accacctctc agaacacgct ggccgcgctg gtcgagctgg atcagaggat cctgatcgtc 120

ggctgcgacc cgaaggccga ctcgacccgc ctgatcctgc acgcaaaggc ccaggacacc 180

gtcctgcatc tggccgccga ggccggctcg gtcgaggatc tggagctcga ggacgttctc 240

aagatcggct acaagaacat caagtgcgtc gagtccggcg gtccggagcc gggggtcggc 300

tgcgccggcc gcggcgtcat cacctcgatc aacttcctgg aagagaacgg cgcctacgac 360

gacgtggact atgtgtccta cgacgtgctg ggcgacgtgg tctgcggcgg cttcgccatg 420

ccgatccgcg agaacaaggc cggaaatc tacatcgtca tgtccggcga gatgatggcg 480

ctgtacgccg ccaacaacat cgccaagggc atcctgaagt acgcgcacag cggcggcgtc 540

cgtctcggcg gcctgatctg caacgagcgc cagaccgaca aggaatggga tctggccgac 600

gcgctggcca agcgcctgggg ctccaagctg atccacttcg tgccgcgcga caacatcgtc 660

cagcacgccg agctgcgccg catgacggtc atcgagtacg ccccggacag caagcaggcc 720

ggcgaatacc gcgcgctcgc caacaagatc catgcgaact ccggcgggg ttgcatcccg 780

accccgatca ccatggaaga gctggaagag atgctgatgg acttcggcat catgaagacc 840

gaggagcagc agctcgccga gctcgccgcc aaggaagcgg cgaaggccgg cgcctga 897

<210> 169

<211> 1440

<212> DNA

<213> Azospirillum lipoferum

<220>

<223> nifD1

<400> 169

atgagcctgt ccgagaacac cacggtcgac gtcaagaacc tcgtcaacga agtcctcgaa 60

gcctatcccg aaaaatcccg caagcgccgc gccaagcacc tgaacgtgct ggaggccgag 120

gccaaggact gcggcgtcaa gtcgaacgtc aagtccatcc ccggcgtcat gaccatccgc 180

ggctgcgcct atgccggctc caagggcgtg gtgtggggtc cgatcaagga catgatccac 240

atctccccg gtccggtcgg ctgcggctac tactcctggt ccggccgccg caactactac 300

atcggcgaca ccggtgtgga cagctggggc acgatgcact tcacctccga cttccaggag 360

aaggacatcg tcttcggcgg cgacaagaag ctgcacaagg tcatcgagga aatcaacgag 420

ctgttcccgc tggtgaacgg catctcgatc cagtcggaat gcccgatcgg cctgatcggc 480

gacgacatcg aggctgtcgc ccgcgccaag tcggcggaaa tcggcaagcc ggtcatcccc 540

gtgcgctgcg aaggcttccg cggcgtgtcc cagtcgctgg gccaccacat cgccaacgac 600

gccatccgag actgggtgtt cgagaagacg gaacccaagg ccggcttcgt ctccaccccc 660

tatgacgtca ccatcatcgg cgactacaac atcggcggcg acgcctggtc gtcccgcatc 720

ctgctggagg agatcggcct gcgcgtgatc gcccagtggt cgggcgacgg cacgctcgcc 780

gaactggaga acacgccgaa ggccaaggtc aacctgatcc actgctaccg ctcgatgaac 840

tacatcgcgc gccacatgga agagaagttc aacattcctt ggatggaata caacttcttc 900

ggcccgagcc agatcgccga atccctgcgc aagatcgccg ctctcttcga cgacaagatc 960

aaggagaacg ccgagaaggt catcgcccgc taccagccga tggtcgatgc ggtcatcgcc 1020

aagtacaagc cgcggctcga aggcaagaag gtcatgatct acgtcggcgg cctgcgtccc 1080

cgccacgtcg tcgatgccta ccatgacctc ggcatggaga tcaccggcac cggctacgag 1140

ttcgcccaca acgacgacta tcagcgcacg cagcactacg tgaaggaagg cacgctgatc 1200

1260

gtcgcgtcgg gcatcaagga aaagtacgtg ttccagaaga tgggcctgcc gttccgccag 1320

atgcacagct gggactactc cggcccgtac cacggctatg acggcttcgc gatcttcgcc 1380

cgcgacatgg acctggccat caacaacccc gtctggggcg tgatgaaggc cccgttctga 1440

<210> 170

<211> 1419

<212> DNA

<213> Azospirillum lipoferum

<220>

<223> nifD2

<400> 170

atgctccagg acaagatcca ggatgtcttc aacgaaccgg gctgcgcgac caaccaagcc 60

aaatcggcca aggagaagaa gaagggctgc accaagtcgc tgaaaccgggg ggcggcagcc 120

ggcggctgcg cctatgacgg ggcgatgatc gtgctccagc cgatcgccga cgccgcccat 180

ctggtccatg gccccatcgc ctgcctcgga aacagttggg acaaccgcgg ctccaaatcc 240

tccggctcgc agctctaccg caccggcttc accaccgatc tgtcggaact ggacgtcatc 300

ggcggcggcg agaagaagct ctaccgcgcc atcaaggaga tcgttcagca atacgacccg 360

ccggccgtct tcgtctatca gacctgcgtg cccgccatga ccggcgacga catcgccgcg 420

gtctgcaagt tcgccacgca gaagctgggc aagccggtga tcccggtgga ctcgccgggc 480

ttcgtcgggt cgaagaatct cggcaacaag ctggccggcg aagccctgct ggagcatgtc 540

atcggcacgg tcgaaccgga ctacaccacc ccgaccgacg tctgcatcat cggcgaatac 600

aaccttgccg gcgagctgtg gctggtcaag ccgctgctgg acgagatcgg catccgcctc 660

ctgtcctgca tttccggcga cggccgctac cgggaggtgg cgcaggccca ccgcgcccgc 720

gtcaccatga tggtgtgcag ccaggcgctg gtgaatgtcg ggcgcaagat ggaggagcgc 780

tacggcatcc cctatttcga ggggtccttc tacggcgtgt ccgacatgtc ggacaccctg 840

cgcaccatga cccgcatgct ggtggagcgc ggcgccgaca agggcctgat cgaccgggcg 900

gagggcgtga tcgcgcggga ggaaagccgg gtctggcgcc ggctggaacc ctacaagccg 960

cgcttcgacg gcaagcgcgt ccttctcttc accggcggcg tcaagagctg gtcgatggtc 1020

agcgcgctgg agggtgcggg gctgaccatc ctcggcacct ccaccaagaa atcgaccagg 1080

gaggacaagg agcgcatcaa gaagatgaag ggcgaagagt tccaccagtg ggacgatttg 1140

aagccgcgcg acatctacag gatgctggcc gacgatcagg ccgacatcat gatgtccggc 1200

ggccgctcgc agttcatctc gctgaaggcc aaggttccct ggctcgacat caaccaggag 1260

cgccaccacg cctatgccgg ctatgacggc atcgtcaatc tctgcgagga gatcgacaaa 1320

acgctgtcga atccgatctg gcgtcaggtg cgtcagccgg caccgtggga gtccggcgcg 1380

tcctccaccc ttctggcttc ctcgatggcg gcggagtga 1419

<210> 171

<211> 1383

<212> DNA

<213> Azospirillum lipoferum

<220>

<223> nifK

<400> 171

atgtcccaca tccagcgctt cccctccgcc gccaaggccg cctccaccaa cccgctgaag 60

atgagccagc cgctgggtgc ggctctggcc tatctcggcg tcgaccgctg cctgccgctg 120

ttccatggct cgcagggctg caccgccttc gggctggtcc tgctggtgcg ccatttccgc 180

gaggcgatcc cgctccagac cacggcgatg gatcaggtcg ccaccatcct cggcggctac 240

gacaatctgg agcaggcgat ccgcaccatc gtcgagcgca accagcccgc catgatcggc 300

gtcgccacca ccggcgtcac cgagaccaag ggcgaggata tggccggaca gtacacgctg 360

ttccgccagc gcaaccccga cttggccgac acggccctgg tcttcgccaa cacccccgac 420

ttcgccggcg gcttcgagga cggcttcgcc gccgcggtca ccgcgatggt cgagcggttg 480

gtcgaaccgt cgccggtgcg catcccgacc caggtcaacg tgctggccgg ctgccatctg 540

tcccccggcg acgtggagga actgcgcgac atcatcgaag gcttcggcct gtcgccgatc 600

ttcctgcccg acctgtcgct gtcgatggcg ggccgccagc cggccgactt caccgccacc 660

tcgctgggcg gcgtgaccgt cgatcagatc cgcgccatgg gcgcttcggc cctcaccatc 720

gtggtcggtg agcatatgcg ggtggccggt aacgcgctgg agctgaagac cgacgtgccc 780

agccatttct tcaaccgcct gaccgggctg gaggcgacgg acaagctggt ccggctgctg 840

atggagttgt cgggcaagcc ggcgcccgcc cggctgcggc gccagcgcga aagcctggtc 900

gatgccatgc tcgacgggca tttcttctac agccgcaagc gcatcgccgt cgcgctggag 960

cccgacctgc tctatgccgt caccggcttc ctcgccgaca tgggggccga ggtgatcgcc 1020

gcggtgtccc cgacgcagag cccggtgctg gagcggttga aggccgccac catcatggtc 1080

ggcgatcatt ccgacgtgga gacgctggcc cgcgacgccg acctgatcgt ctccaactcg 1140

cacgggcggc aggagccgc gcggatcggc gtggctctgc accgcatggg cctgccgctg 1200

ttcgaccggc tgggggccgg cctgcgcgtc caggtcggct accgcggcac gcgggaactg 1260

ctgtgcgaca tcggcaacct gttcctcgcc cgcgagatgg accacgagca cgggcacgag 1320

agccacgacc acggggaatc ccacggctgc ggaggcggat catgcggatg caacgccgtc 1380

tga 1383

<210> 172

<211> 1560

<212> DNA

<213> Azospirillum lipoferum

<220>

<223> nifK1

<400> 172

atgaccgaca agctttcgca gagcgccgac aaggtcctcg accactacac cctcttccgg 60

cagcccgaat acgcggcgat gttcgagaag aagaagaccg agttcgagta cggccattcg 120

gacgaggaag tcgcccgcgt gtccgaatgg accaagtccg aggactacaa ggcgaagaac 180

ttcgcccgtg aagcggtcgt catcaacccg accaaggcct gccagccgat cggcgcaatg 240

ttcgccgccc agggcttcga aggcaccctg cccttcgtcc acggctccca gggctgcgtc 300

gcctattacc gcacccacct gacccgtcac ttcaaggagc cgaacagcgc ggtctcctcg 360

tcgatgacgg aggacgcggc ggtgttcggc ggcctgaaca acatgatcga cggcctggcg 420

aacgcctatg cgctctacaa gccgaagatg atcgcggtga tgaccacctg catggccgaa 480

gtcatcggcg acgatttgca gggcttcatc gccaatgcga agaccaagga cagcgtcccg 540

gccgacttcc cggtccccta cgcccacacc ccggccttcg tcggcagcca catcgtcggc 600

tacgacaaca tgatcaaggg gatcctgacc aacttctggg gtacgtcgga gaatttcgac 660

acacccaaga ccgagcagat caacctgatc ccgggcttcg acggcttcgc cgtcggcaac 720

aaccgcgaac tgaagcgcat cgccggcgaa ttcggcgtga agctgcaaat cctgtccgac 780

gtgtccgaca atttcgacac gccgatggat ggcgagtacc gcatgtatga cggcggcacc 840

accatcgagg agaccaagga ggccctgcac gccaaggcca ccatctccat gcaggagtac 900

aacacgaccc agaccctgca attctgcaag gagaagggtc aggaagtcgc caagttcaac 960

tacccgatgg gcgtcaccgg caccgacgag ctgctgctga agctcgccga actgtcgggc 1020

aagccggtcc cggccagcct gaagctggag cgcggccgtc tggtcgacgc catcgccgac 1080

agccacaccc acatgcacgg caagcgcttc gccgtctatg gcgacccggga cttctgcctg 1140

ggcatgtcca agttcctgct ggagctgggt gcggagccgg tgcacatcct gtcgacgtcg 1200

ggctccaaga agtgggagaa gcaggtccag aaggtgctgg acggctcgcc cttcggcgcc 1260

tcgggcaagg cccatggcgg caaggatctg tggcacctgc gttcgctgat cttcaccgac 1320

aaggtggact acatcatcgg caacagctac ggcaagtatc tggagcgcga caccaaggtt 1380

ccgctgatcc gcctgaccta cccgatcttc gaccgccacc accaccaccg ctacccgacc 1440

tggggctacc agggcgcgct gaacgtgctg gtacggatcc tggaccggat cttcgaggac 1500

atcgacgcca acaccaacat cgtcggccag accgactact cgttcgacct gatccgctga 1560

<210> 173

<211> 1530

<212> DNA

<213> Azospirillum lipoferum

<220>

<223> nifA

<400> 173

atgttgacct ctgatattgt tggcaaattg cgctgcatcg cagcagaccc caaagcgggc 60

atcgcaaggg gcctcgacac cgggacgacg aagatcggtc ccgtttggga gggtgacgtg 120

ggcgacaccg tggatttcga agcgctgcgc cagcgggcgg tccactccct gttcgaacat 180

ctggaatcca tgtgcgtcgg cgccgtcgcc gtcgaccaca ccggccgcat cgcctggatg 240

gacgagaagt acaaggctct gctgggcgtt cccgacgacc cgcgcggccg gcaggtggag 300

gacgtcatcc ccaacagcca gctgcgccgg gtgatcgaca gcggccagcc gcagccgctg 360

gacatcatgg agttcgacga ccggtccttc gtggtgacgc ggatgccgct gttcggcacc 420

gacggttcga tcatcggcgc catcggcttc gtgctgttcg accgcgccga atatctccgc 480

ccgctggtcc gcaaatacga gaagatgcag gaggagctgg cccgcaccca gcaggagctg 540

gcgcatgagc gccgcgccaa atactccttc tcgcagttcc tgggcgccag cgaatcgatc 600

cgcgagatca agcggctggg gcgccgcgcc gcccagatgg attcgaccgt cctgctgctg 660

ggcgaaaccg ggaccggcaa ggagctgctg gcccaggcca tccattccgc cagcccgcgg 720

gcgtccaagc ccttcgtcgg cgtcaatgtc gccgccattc cggaaaccct gctggaggcg 780

gagttcttcg gcgtcgcccc cggcgccttc accggcgccg accgccgcca ccgcgacggc 840

aagttccagc tcgccaacgg cggcaccctg ttcctcgacg agatcggcga catgccgctg 900

ccggtgcagg ccaagcttct gcgcgtgctg caggagcggg agatcgagcc gctcggctcc 960

1020

ctggtgcgtg agaagcagtt ccgcgccgac ctctattacc ggctgaatgt ggtgccgatc 1080

accctgccgc cgctgcgcga ccggccggag gacatcgaga gcatcgccga ccgcatcctg 1140

gaacagctgg cgatccagca gggcacgccg ccgcgcgagc tgctggaatc ggcggtgcag 1200

gtgctgcgcg actatgactg gcccggcaat gtgcgcgagc tttacaacac gctggaacgg 1260

gtggtggcgc tgaccgatgc gccgatcctg accgcgccgc acatccgcag cgtgctgccc 1320

ggccagcatc cggccggcgc gtcggccctg ccgctggcgg ccggcgcgcg gccgttgcag 1380

gaggtgctgc acgccgccga gcgccacgcc atcgccgcgg cgcttgagga ggcgaacggc 1440

gtcaaggcgc gggcggcgaa gctgctgggc atttcgcgcg cgtcgctgta cgaacgcatg 1500

gtgacgctgg ggttgggggc gacgcagtag 1530

<210> 174

<211> 3030

<212> DNA

<213> Azospirillum lipoferum

<220>

<223> glnE

<400> 174

atgccgagtc ccatcgcgtt ctcaagcccc ttgccgaagc ctttcgacag cgcgcaggcg 60

gcgctgggga tggagcgctg gcgccagcag gccgccgcgg cggagccgga gacccgcgcc 120

tgggcggaag ccttcgccga ttcggagacc ggccgggcgc tgatcggggc ggtgtgcggc 180

aacagcccgt atctcggcca cagcctgacg cgggagttgc ccttcgtcgc ccgtacagtg 240

caggacggct tcgacgacac cttcgccgcg ctgatcgccg ctctccatgc cgagcatggc 300

gaggagaaat cgatggaccg gctgatggcc ggcctgcggg tggcgaagcg gcgggcggcg 360

ctgctgatcg cgctggccga catcgccggc gcgtggccgc tgttccgcgt caccggcgcc 420

ctgtcggagc tggcggagac gggggtgcag ctggccgcga atttcctgct gcgccgcgcc 480

agggaggcgg ggacgctgac gctgccggat ccgcagcgac cgtgggtcgg ttcgggcctg 540

atcgttttgg gcatgggtaa gcttggcggg cgcgaactca actattccag cgacatcgac 600

ctgatcgtcc tgtatgacga cgctgttgtg cagacgcccc agccggacaa cctcgcgcga 660

accttcatca ggctcgcacg cgatcttgtc cgcattatgg atgaacggac caaggacggc 720

tacgtcttcc gcaccgacct tcggcttagg cccgatcccg gcgccacgcc gctggcggtt 780

tccgtctccg cagccgaaat ttattacggc agcgtcggtc agaactggga acgcgcggcg 840

atgatcaagg cccgtcccat cgccggcgat ctggaggcgg gcgcctcctt tgtccgcttc 900

ctggagccct tcgtctggcg ccgcaacctg gatttcgccg ccatccagga catccattcg 960

atcaaacgcc agatcaacgc ccacaagggc caccgcgagg tgacggtcaa cggccacgac 1020

atcaaggtcg gccgcggcgg catccgcgag atcgagttct tcgcccagac ccagcagctg 1080

atcttcggcg ggcgcgaccc gcgcgtgcga atcgctccga ccctgatggc gaacgaggcg 1140

ctgcgcgacg tcggccgcgt gccgccgcag acggtggaag agcttgccgg ggcctatcat 1200

ttcctgcgcc gtgtcgaaca tcgcatccag atgatcgacg accagcagac ccatcgtatt 1260

cccgccgacg atgccggggt ggcgcatttg gccaccttcc tcggctatga cgaccccgcc 1320

1380

ttcgaggagg cgccgtcgct ttccggcccc ggcaatctgg tcttcaccgg caccgacccc 1440

gatccgggca cgatggagac gctgaagggc atgggcttcg ccgatccggc ccgcgtcatc 1500

agcgtggtgt cggcctggca tcgcggccgc taccgcgcca cccggtcggg cggggcgcgg 1560

gagctgctga cggagctgac gccggccctg ctgagtgcgc tgaccaagac cccggccccc 1620

1680

ttctcgctgt tcgtcgccaa tccctggctc ctggagctgg tggcggagat catgggcatc 1740

gcgccgcaga tggcgcagac gctgtcgcgc aacccgtcgc tgctcgacgc cgtgctgtcg 1800

ccggacttct tcgacccgct gccgggcaag gaggacgggc tggccgacga ccacgcccgc 1860

gtgatggcgc cggcccgcga tttcgaggat gcgctgaccc tgtcgcggcg ctggaccaac 1920

gaccagcgct tccgcgccgg ggtgcatatc ctgcgcggca tcaccgatgg cgaccgctgc 1980

ggcgccttcc tggccgatct ggccgacatc gtcgtccccg accttgcccg ccgggtggag 2040

gaggagttcg cccagcgcca cggccatatt cccggcggcg cctgggtggt ggtggcgatg 2100

ggcaagctcg gcagccggca gctgaccatc acgtccgaca tcgacctgat cgtcatctac 2160

gatgtggcgc cgggccaagg gggcgggggc ggtccccgct tgtcggatgg tgccaagccg 2220

ctgtcgccca acgagtatta catcaagctg actcagcgtc tgaccaacgc cattaccgcg 2280

ccgatgggcg acggccggct ctacgaggtc gacatgcggc tgcgcccgtc gggcaacgcc 2340

gggccgctcg ccacctcgct ggacgctttc ctgaaatatc aggcgaccga tgcctggacc 2400

tgggagcata tggccctgac ccgcgcccgg gtgatcggcg gtgatgcgga gctggccggg 2460

cgggtgtcgg cagcgatccg ctcggtgctg acggcgccgc gcgatgccga ccggctgctg 2520

tgggacgtgg ccgacatgcg gcggcggatc gagaaggagt tcgggacgac caatgtctgg 2580

aacgtcaaat acgcccgcgg cggcctgatc gacatcgagt tcatcgccca gtacctgcaa 2640

ctgcgccatg gtcacgagcg gccggacatc ctgcacatcg gcaccgccaa ggcgctggggc 2700

tgcgccgccc ggacgggcgc gctggcgccg gaggtggcgg aggatctgga gacgacgctg 2760

cggctgtggc ggcgggtgca gggctttctg cggttgacca ccgccggggt gctcgatccc 2820

aatcaggtgt cgcccagcct gctggccggg ctggtccgcg ccgcctttcc tgctgacttt 2880

cagggcgagc gtgagcctgg cactgttgac ttccccgaac tggaccacaa aatccgtgcc 2940

gtcgccgccc gcgcccatgg tcatttcaag accctggtcg aggaaccggc gggccgtctg 3000

gccccacccg ccaccacgcc tccagcctga 3030

<210> 175

<211> 1440

<212> DNA

<213> Azospirillum lipoferum

<220>

<223> amtB1

<400> 175

atgaaccgtc tgttccttat ggccgcaccg atgatggcgg ttgctctgggg cgcggtcggc 60

atgccggccg cagcccttgc ccaggatccg gcggctgccg ccgctgccgc ggctgcggct 120

gcggctgccg ccgctgctgc cgcaccggcg gctccggcgc tgaatggcgg cgacaccgcc 180

tggatgctca tctccaccgc gctggtgctg atgatgacca tccccggcct ggcgctgttc 240

tacggcggca tggtccgcaa gatgaacgtg ctgtcgacgg tgatgcagag cttcgccatc 300

acctgcctga tcagcgtcct gtggtacgtc atcggctaca gcctggcctt caccggcacc 360

ggtgcctatg tcggcggtct cgaccggctg ttcctcaacg ggctcgactt cacgaaggcc 420

ttcgtgctgg gcgaggcgac cgggtcgggc gtcccgacga ccatccccga gccggtcttc 480

atgatgttcc agatgacctt tgcgatcatc accccggccc tgatcaccgg cgccttcgcc 540

gaccgcatga agttctcctc cctgctggtc ttcaccgcgc tgtggtcgat cgtggtctat 600

660

gacttcgccg gcggcacggt cgtgcacatc aacgccggcg tcgccggcct ggtcgccgcg 720

ctggtgatcg gcaagcgcaa gggctacccg aaggaagcct tcatgccgca caacctggtg 780

ctgtcgctga tcggcgcctc gctgctgtgg gtcggctggt tcggcttcaa cgccggttcg 840

gccctgaccg ccggtccgcg tgccggcatg gcgctggccg ccacgcacat cgccaccgcc 900

ggtgccgcca tgggctggct gttcgcggag tggatcgtca agggcaagcc gtcgatcctc 960

ggcatcatct ccggcgccgt cgccggcctg gtcgcggtga ccccggccgc cggcttcgtc 1020

gacccgacgg gcgccatcgt catcggcatc gtcgccggcg tggtctgctt ctggtcggcc 1080

accagcctca agcacatgct gggctatgac gacagcctgg acgccttcgg cgtgcacggc 1140

gtcggcggcc tgatcggcgc catcctgacc ggcgtcttcg ccaagatgtc ggtgtccaac 1200

agcgaaggcg gcttcgcctc cgtcctgcag gccgacccga aggccacgct gggcctgctg 1260

gaaggcaacg ccgccgccgt ctggatccag gtccagggcg tcctctacac catggtctgg 1320

tgcgccatcg ccaccttcgt cctgctgaag atcgtcgatg tggtcatggg cctgcgcgtc 1380

gaagaggatg tggagcgcga cggtctcgac ctcgccctgc atggcgagag catccactaa 1440

<210> 176

<211> 1227

<212> DNA

<213> Azospirillum lipoferum

<220>

<223> amtB2

<400> 176

atggatgcgg caaagacggg tggcgacgtc cttttcgtgc tgatgggcgc ggtgatggtg 60

ctggcgatgc attgcggctt cgccctgctg gaggtcggga cggtccggcg caagaatcag 120

gtcaacgcgc tggtgaagat cctgtcggac ttcgccatgt cgaccatcgc ctattttttc 180

gtcggttatg ccgtggccta cggcatcgac ttcttcgccg acgcccacac gctggtcggc 240

aagggaagcg gcgggttcgc ggcctatggc tacgatctgg tgaagttctt cttcctggcg 300

360

tggccgcagg ccgccgccac gctggcgctg atcgcgctgt tctatccatt gctggaaggc 420

acggtctggg gcacccgctt cggcctgcaa agctggatgg ccgcgacctt cggccagcct 480

ttccacgact tcgccggatc tgtggtggtg catgccttcg gcggctgggt ggcgctgggt 540

gccgtgctga acctcggcaa ccgccgcggc cgctaccgtc cgaacggctc gctgatcgcc 600

attccgccgt cgaacatccc cttcctggcg ctgggcgcct gggtgctgtg cgtggggtgg 660

ttcggcttca acgtgatgag cgcccaggtg ctggatggcg tgacgggtct ggtggcgctg 720

aactcgctga tggcgatggt cggcggcatc gtcacctcgc tggtgatcag ccgcaccgat 780

cccggcttcg tccacaacgg cgcgctggcc ggtctggtgg cggtctgcgc cgggtccgac 840

gtgatgcacc cgctgggcgc gctggtcacc ggcggcatcg ccgggctgct gttcgtctgg 900

gccttcaaca aatgccagat cgactggaag atcgacgacg tgctgggcgt ctggccgctg 960

cacggtctgt gcggcctgac cggcggcctg ctggccggcg tcttcgggca ggaggcactg 1020

ggcggccttg gcggcgtgtc gatcctcagc cagatcgtcg gcacggcaag cggcgccagc 1080

ttcggattcg tctcgggtct ggcggtctac ggcctgctgc gcgtcaccgt cggcatccgc 1140

ctcgatcccg agcaggagta caagggcgcc gacttgtcgt tgcaccatat caccgcgtac 1200

ccggaagagg acgcgccgac cctgtaa 1227

<210> 177

<211> 613

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::PinfC

<400> 177

atgaccctga atatgatgat ggattcttgg ttctctggag cgctttatcg gcatcctgac 60

tgaagaattt gcaggcttct tcccaacctg gcttgcaccc gtgcaggtag ttgtgatgaa 120

catcactgat tcgcaggctg aatacgttaa cgaattgacc cgtaaactgc aaaatgcggg 180

cattcgtgta aaagcagact tgagaaacga gaagattggc tttaaaatcc gcgagcacac 240

tttacgtcgt gtcccttata tgctggtttg tggtgacaaa gaggtcgaag ccggcaaagt 300

tgctgtgcgt acccgtcgcg gtaaagacct gggtagcctg gacgtaaatg atgttatcga 360

gaagctgcaa caagagattc gcagccgcag tcttcaacaa ctggaggaat aaggtattaa 420

aggcggaaaa cgagttcaaa cggcgcgtcc caatcgtatt aatggcgaga ttcgcgccac 480

ggaagttcgc ttaacaggtc tggaaggcga gcagcttggt attgcgatag aactcacttc 540

acgccccgaa gggggaagct gcctgaccct acgattcccg ctatttcatt cactgaccgg 600

aggttcaaaa tga 613

<210> 178

<211> 1613

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::PinfC with a 500 bp flanking region.

<400> 178

accggataag agagaaaagt gtcgacgtcg gtccggttga tattgaccgg cgcatccgcc 60

agctcgccca gtttttggtg gatctgtttg gcgattttgc gggtcttgcc ggtgtcggtg 120

ccgaaaaaaa taccaatatt tgccataaca cacgctcctg ttgaaaaaga gatcccgcgg 180

ggaaaatgcg gtgaacatgt cagctattgc gaagagtgtg ccagttttgc tcacgggcaa 240

aagctgcacc agaatgggta ttaatgcacc agcctggcgc tttttttcgc ggcacgtccc 300

ctcgctaatg cccgtctggc gcggctttga cgctgataag gcgctgaata ccgatctgga 360

tcaaggtttt gtcgggttat cgtccaaaag gtgcactctt tgcatggtta taagtgcctg 420

acatggtgtc cgggcgaacg tcgccaggtg gcacaaattg tcagaactac gacacgacta 480

accgaccgca ggagtgtgcg atgaccctga atatgatgat ggattcttgg ttctctggag 540

cgctttatcg gcatcctgac tgaagaattt gcaggcttct tcccaacctg gcttgcaccc 600

gtgcaggtag ttgtgatgaa catcactgat tcgcaggctg aatacgttaa cgaattgacc 660

cgtaaactgc aaaatgcggg cattcgtgta aaagcagact tgagaaacga gaagattggc 720

tttaaaatcc gcgagcacac tttacgtcgt gtcccttata tgctggtttg tggtgacaaa 780

gaggtcgaag ccggcaaagt tgctgtgcgt acccgtcgcg gtaaagacct gggtagcctg 840

gacgtaaatg atgttatcga gaagctgcaa caagagattc gcagccgcag tcttcaacaa 900

ctggaggaat aaggtattaa aggcggaaaa cgagttcaaa cggcgcgtcc caatcgtatt 960

aatggcgaga ttcgcgccac ggaagttcgc ttaacaggtc tggaaggcga gcagcttggt 1020

attgcgatag aactcacttc acgccccgaa gggggaagct gcctgaccct acgattcccg 1080

ctatttcatt cactgaccgg aggttcaaaa tgacccagcg aaccgagtcg ggtaataccg 1140

tctggcgctt cgatttatcc cagcagttca ccgcgatgca gcggataagc gtggttctca 1200

gccgggcgac cgaggttgaa cagacactcc agcaggtgct gtgcgtattg cacaatgacg 1260

cctttttgca gcacggcatg atctgtctgt acgacagcca gcaggcgatt ttgactattg 1320

aagcgttgca ggaagccgat cagcagttga tccccggcag ctcgcaaatt cgctaccgtc 1380

cgggtgaagg gctggtcggg acggtgcttt cgcaggggca atcgttagtg ctggcgcgtg 1440

tggctgacga tcagcgcttt cttgaccgcc tgggactgta tgattacaac ctgccgttta 1500

tcgccgtgcc gctgataggg ccggatgcgc agacttttgg cgtgctgacg gcgcaaccga 1560

tggcgcgtta cgaagagcgg ttacccgcct gcacccgctt tctggaaacg gtc 1613

<210> 179

<211> 1185

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2

<400> 179

tccctgtgcg ccgcgtcgcc gatggtggcc agccaactgg cgcgctaccc gatcctgctc 60

gatgaactgc tcgacccgaa cacgctctat caaccgacgg cgatgaacgc ctatcgcgat 120

gaactgcgac aatacctgtt gcgcgtgccg gaagaggatg aagagcagca actggaggcg 180

ctacggcagt ttaagcaggc gcagttgttg cgcgtagcgg cggcggatat cgccggtacg 240

ttacccgtca tgaaagtgag cgatcactta acctggctgg cggaagcgat tatcgatgcg 300

gtggtgcagc aagcctggaa ccagatggtg gcgcgttacg gccagccgac gcatctgcac 360

gatcgcgaag ggcgcggttt cgccgtggtc ggttacggca aacttggcgg ctgggaatta 420

ggttacagct ccgatctgga tctggtgttc ctgcacgact gccccatgga tgtgatgacc 480

gatggcgagc gtgaaatcga tggccgccag ttctatttgc gcctcgcgca gcgcgtgatg 540

cacctgttca gcacgcgcac gtcgtccggc attctttatg aagtcgatgc gcgtttgcgc 600

660

aatgaagcct ggacatggga gcatcaggcg ctggcgcgtg cgcgcgtggt gtacggcgat 720

ccgcaactga ccgccgaatt tgacgccatt cgccgcgata tcctgatgac ctcccgcgat 780

gccgctaccc tgcaaaccga agtgcgggaa atgcgtgaga aaatgcgcgc ccatcttggt 840

aacaagcaca aagaccgttt cgatctgaaa gccgatgaag gcggtatcac cgatattgag 900

tttatcgctc agtatctggt gctgcgcttt gcccatgaga agccgaaact gacgcgctgg 960

tcggataatg tgcgcatcct cgaagggctg gcgcaaaacg gcatcatgga tgagcaggaa 1020

gcgcaggcat tgacgctggc gtacaccacg ttgcgtgatg agctgcacca cctggcgctg 1080

1140

accagctggg acaagtggct ggtggaaccg tgcgccccgg cgtaa 1185

<210> 180

<211> 2085

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2 with a 500 bp flanking region.

<400> 180

taaagcgagc gctcacttac gtgatctgtt gacgcagtcc gaagcgacca ttacttcagc 60

cgtttcagca gatacggcgg tgtggagtgc gcaatcagcc ctggcgaaac tggtgctcac 120

cgagtggtta gtgacgcagg gctggcgaac cttccttgat gaaaaagcgc aggctaagtt 180

tgccgactcc tttaaacgct ttgctgacgt tcatctgtca cgcagcgccg ccgagctgaa 240

aaaagccttt gcccagccgc tgggcgacag ctatcgcgac cagttaccgc ggctggcgcg 300

tgatatcgac agcgcgttat tgctggccgg acattacgat cgcgcgcgcg ccgtggagtg 360

gctggaaaac tggcaggggc ttcagcacgc tattgaaacg cgccagagag ttgaaatcga 420

acatttccgt aataccgcca ttacccagga gccgttctgg ttgcacagcg gaaaacgtta 480

acgaaaggat atttcgcatg tccctgtgcg ccgcgtcgcc gatggtggcc agccaactgg 540

cgcgctaccc gatcctgctc gatgaactgc tcgacccgaa cacgctctat caaccgacgg 600

cgatgaacgc ctatcgcgat gaactgcgac aatacctgtt gcgcgtgccg gaagaggatg 660

aagagcagca actggaggcg ctacggcagt ttaagcaggc gcagttgttg cgcgtagcgg 720

cggcggatat cgccggtacg ttacccgtca tgaaagtgag cgatcactta acctggctgg 780

840

gccagccgac gcatctgcac gatcgcgaag ggcgcggttt cgccgtggtc ggttacggca 900

aacttggcgg ctgggaatta ggttacagct ccgatctggga tctggtgttc ctgcacgact 960

gccccatgga tgtgatgacc gatggcgagc gtgaaatcga tggccgccag ttctatttgc 1020

gcctcgcgca gcgcgtgatg cacctgttca gcacgcgcac gtcgtccggc attctttatg 1080

aagtcgatgc gcgtttgcgc ccgtccggcg cggccggaat gctggtgacc actgcggaag 1140

cgttcgccga ttatcaaaaa aatgaagcct ggacatggga gcatcaggcg ctggcgcgtg 1200

cgcgcgtggt gtacggcgat ccgcaactga ccgccgaatt tgacgccatt cgccgcgata 1260

tcctgatgac ctcccgcgat gccgctaccc tgcaaaccga agtgcgggaa atgcgtgaga 1320

aaatgcgcgc ccatcttggt aacaagcaca aagaccgttt cgatctgaaa gccgatgaag 1380

gcggtatcac cgatattgag tttatcgctc agtatctggt gctgcgcttt gcccatgaga 1440

agccgaaact gacgcgctgg tcggataatg tgcgcatcct cgaagggctg gcgcaaaacg 1500

gcatcatgga tgagcaggaa gcgcaggcat tgacgctggc gtacaccacg ttgcgtgatg 1560

agctgcacca cctggcgctg caagagctgc caggacatgt ggcgctctcc tgttttgtcg 1620

ccgagcgtgc gcttatcaaa accagctggg acaagtggct ggtggaaccg tgcgccccgg 1680

cgtaagtgtg gtatcatcgc gcgcaaattt tgtatctctc aggagacagg aatgaaagtt 1740

acgctgccag agttcaatca agccggtgtc atggtggtgg gtgatgtgat gctggatcgc 1800

tactggtacg gcccaaccag ccgcatttct ccggaagcgc cagttccggt tgttaaagtc 1860

gatactattg aagagcgacc gggcggtgcg gcaaacgtgg cgatgaacat tgcctcgctg 1920

ggcgcaacgg cgcgtctggt tggcctgact ggcattgatg atgcggcgcg cgcgctgagc 1980

aaagcgctgg cggatgttaa tgttaaatgt gacttcgttt ctgttccgac tcaccccacc 2040

atcactaagc tgcgcgtgct gtcgcgtaac cagcaactga ttcgc 2085

<210> 181

<211> 461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1

<400> 181

atgaccctga atatgatgat ggatgccagc cgttctgtaa taataaccgg acaattcgga 60

ctgattaaaa aagcgccctc gcggcgcttt ttttatattc tcgactccat ttaaaataaa 120

aaatccaatc ggatttcact atttaaactg gccattatct aagatgaatc cgatggaagc 180

240

cgatcaaatt taagtggatt cccatcaaaa aaatattctc aacctaaaaa agtttgtgta 300

atacttgtaa cgctacatgg agattaactc aatctagagg gtattaataa tgaatcgtac 360

taaactggta ctgggcgcaa ctcacttcac gccccgaagg gggaagctgc ctgaccctac 420

gattcccgct atttcattca ctgaccggag gttcaaaatg a 461

<210> 182

<211> 1461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1 with a 500 bp flanking region.

<400> 182

accggataag agagaaaagt gtcgacgtcg gtccggttga tattgaccgg cgcatccgcc 60

agctcgccca gtttttggtg gatctgtttg gcgattttgc gggtcttgcc ggtgtcggtg 120

ccgaaaaaaa taccaatatt tgccataaca cacgctcctg ttgaaaaaga gatcccgcgg 180

ggaaaatgcg gtgaacatgt cagctattgc gaagagtgtg ccagttttgc tcacgggcaa 240

aagctgcacc agaatgggta ttaatgcacc agcctggcgc tttttttcgc ggcacgtccc 300

ctcgctaatg cccgtctggc gcggctttga cgctgataag gcgctgaata ccgatctgga 360

tcaaggtttt gtcgggttat cgtccaaaag gtgcactctt tgcatggtta taagtgcctg 420

acatggtgtc cgggcgaacg tcgccaggtg gcacaaattg tcagaactac gacacgacta 480

accgaccgca ggagtgtgcg atgaccctga atatgatgat ggatgccagc cgttctgtaa 540

taataaccgg acaattcggga ctgattaaaa aagcgccctc gcggcgcttt ttttatattc 600

tcgactccat ttaaaataaa aaatccaatc ggatttcact atttaaactg gccattatct 660

aagatgaatc cgatggaagc tcgctgtttt aacacgcgtt ttttaacctt ttattgaaag 720

tcggtgcttc tttgagcgaa cgatcaaatt taagtggatt cccatcaaaa aaatattctc 780

aacctaaaaa agtttgtgta atacttgtaa cgctacatgg agattaactc aatctagagg 840

gtattaataa tgaatcgtac taaactggta ctgggcgcaa ctcacttcac gccccgaagg 900

gggaagctgc ctgaccctac gattcccgct atttcattca ctgaccggag gttcaaaatg 960

acccagcgaa ccgagtcggg taataccgtc tggcgcttcg atttatccca gcagttcacc 1020

gcgatgcagc ggataagcgt ggttctcagc cgggcgaccg aggttgaaca gacactccag 1080

caggtgctgt gcgtattgca caatgacgcc tttttgcagc acggcatgat ctgtctgtac 1140

gacagccagc aggcgatttt gactattgaa gcgttgcagg aagccgatca gcagttgatc 1200

cccggcagct cgcaaattcg ctaccgtccg ggtgaagggc tggtcgggac ggtgctttcg 1260

caggggcaat cgttagtgct ggcgcgtgtg gctgacgatc agcgctttct tgaccgcctg 1320

ggactgtatg attacaacct gccgtttatc gccgtgccgc tgatagggcc ggatgcgcag 1380

acttttggcg tgctgacggc gcaaccgatg gcgcgttacg aagagcggtt acccgcctgc 1440

acccgctttc tggaaacggt c 1461

<210> 183

<211> 1185

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2

<400> 183

tccctgtgcg ccgcgtcgcc gatggtggcc agccaactgg cgcgctaccc gatcctgctc 60

gatgaactgc tcgacccgaa cacgctctat caaccgacgg cgatgaacgc ctatcgcgat 120

gaactgcgac aatacctgtt gcgcgtgccg gaagaggatg aagagcagca actggaggcg 180

ctacggcagt ttaagcaggc gcagttgttg cgcgtagcgg cggcggatat cgccggtacg 240

ttacccgtca tgaaagtgag cgatcactta acctggctgg cggaagcgat tatcgatgcg 300

gtggtgcagc aagcctggaa ccagatggtg gcgcgttacg gccagccgac gcatctgcac 360

gatcgcgaag ggcgcggttt cgccgtggtc ggttacggca aacttggcgg ctgggaatta 420

ggttacagct ccgatctgga tctggtgttc ctgcacgact gccccatgga tgtgatgacc 480

gatggcgagc gtgaaatcga tggccgccag ttctatttgc gcctcgcgca gcgcgtgatg 540

cacctgttca gcacgcgcac gtcgtccggc attctttatg aagtcgatgc gcgtttgcgc 600

660

aatgaagcct ggacatggga gcatcaggcg ctggcgcgtg cgcgcgtggt gtacggcgat 720

ccgcaactga ccgccgaatt tgacgccatt cgccgcgata tcctgatgac ctcccgcgat 780

gccgctaccc tgcaaaccga agtgcgggaa atgcgtgaga aaatgcgcgc ccatcttggt 840

aacaagcaca aagaccgttt cgatctgaaa gccgatgaag gcggtatcac cgatattgag 900

tttatcgctc agtatctggt gctgcgcttt gcccatgaga agccgaaact gacgcgctgg 960

tcggataatg tgcgcatcct cgaagggctg gcgcaaaacg gcatcatgga tgagcaggaa 1020

gcgcaggcat tgacgctggc gtacaccacg ttgcgtgatg agctgcacca cctggcgctg 1080

1140

accagctggg acaagtggct ggtggaaccg tgcgccccgg cgtaa 1185

<210> 184

<211> 2085

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2 with a 500 bp flanking region.

<400> 184

taaagcgagc gctcacttac gtgatctgtt gacgcagtcc gaagcgacca ttacttcagc 60

cgtttcagca gatacggcgg tgtggagtgc gcaatcagcc ctggcgaaac tggtgctcac 120

cgagtggtta gtgacgcagg gctggcgaac cttccttgat gaaaaagcgc aggctaagtt 180

tgccgactcc tttaaacgct ttgctgacgt tcatctgtca cgcagcgccg ccgagctgaa 240

aaaagccttt gcccagccgc tgggcgacag ctatcgcgac cagttaccgc ggctggcgcg 300

tgatatcgac agcgcgttat tgctggccgg acattacgat cgcgcgcgcg ccgtggagtg 360

gctggaaaac tggcaggggc ttcagcacgc tattgaaacg cgccagagag ttgaaatcga 420

acatttccgt aataccgcca ttacccagga gccgttctgg ttgcacagcg gaaaacgtta 480

acgaaaggat atttcgcatg tccctgtgcg ccgcgtcgcc gatggtggcc agccaactgg 540

cgcgctaccc gatcctgctc gatgaactgc tcgacccgaa cacgctctat caaccgacgg 600

cgatgaacgc ctatcgcgat gaactgcgac aatacctgtt gcgcgtgccg gaagaggatg 660

aagagcagca actggaggcg ctacggcagt ttaagcaggc gcagttgttg cgcgtagcgg 720

cggcggatat cgccggtacg ttacccgtca tgaaagtgag cgatcactta acctggctgg 780

840

gccagccgac gcatctgcac gatcgcgaag ggcgcggttt cgccgtggtc ggttacggca 900

aacttggcgg ctgggaatta ggttacagct ccgatctggga tctggtgttc ctgcacgact 960

gccccatgga tgtgatgacc gatggcgagc gtgaaatcga tggccgccag ttctatttgc 1020

gcctcgcgca gcgcgtgatg cacctgttca gcacgcgcac gtcgtccggc attctttatg 1080

aagtcgatgc gcgtttgcgc ccgtccggcg cggccggaat gctggtgacc actgcggaag 1140

cgttcgccga ttatcaaaaa aatgaagcct ggacatggga gcatcaggcg ctggcgcgtg 1200

cgcgcgtggt gtacggcgat ccgcaactga ccgccgaatt tgacgccatt cgccgcgata 1260

tcctgatgac ctcccgcgat gccgctaccc tgcaaaccga agtgcgggaa atgcgtgaga 1320

aaatgcgcgc ccatcttggt aacaagcaca aagaccgttt cgatctgaaa gccgatgaag 1380

gcggtatcac cgatattgag tttatcgctc agtatctggt gctgcgcttt gcccatgaga 1440

agccgaaact gacgcgctgg tcggataatg tgcgcatcct cgaagggctg gcgcaaaacg 1500

gcatcatgga tgagcaggaa gcgcaggcat tgacgctggc gtacaccacg ttgcgtgatg 1560

agctgcacca cctggcgctg caagagctgc caggacatgt ggcgctctcc tgttttgtcg 1620

ccgagcgtgc gcttatcaaa accagctggg acaagtggct ggtggaaccg tgcgccccgg 1680

cgtaagtgtg gtatcatcgc gcgcaaattt tgtatctctc aggagacagg aatgaaagtt 1740

acgctgccag agttcaatca agccggtgtc atggtggtgg gtgatgtgat gctggatcgc 1800

tactggtacg gcccaaccag ccgcatttct ccggaagcgc cagttccggt tgttaaagtc 1860

gatactattg aagagcgacc gggcggtgcg gcaaacgtgg cgatgaacat tgcctcgctg 1920

ggcgcaacgg cgcgtctggt tggcctgact ggcattgatg atgcggcgcg cgcgctgagc 1980

aaagcgctgg cggatgttaa tgttaaatgt gacttcgttt ctgttccgac tcaccccacc 2040

atcactaagc tgcgcgtgct gtcgcgtaac cagcaactga ttcgc 2085

<210> 185

<211> 461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1

<400> 185

atgaccctga atatgatgat ggatgccagc cgttctgtaa taataaccgg acaattcgga 60

ctgattaaaa aagcgccctc gcggcgcttt ttttatattc tcgactccat ttaaaataaa 120

aaatccaatc ggatttcact atttaaactg gccattatct aagatgaatc cgatggaagc 180

240

cgatcaaatt taagtggatt cccatcaaaa aaatattctc aacctaaaaa agtttgtgta 300

atacttgtaa cgctacatgg agattaactc aatctagagg gtattaataa tgaatcgtac 360

taaactggta ctgggcgcaa ctcacttcac gccccgaagg gggaagctgc ctgaccctac 420

gattcccgct atttcattca ctgaccggag gttcaaaatg a 461

<210> 186

<211> 1461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1 with a 500 bp flanking region.

<400> 186

accggataag agagaaaagt gtcgacgtcg gtccggttga tattgaccgg cgcatccgcc 60

agctcgccca gtttttggtg gatctgtttg gcgattttgc gggtcttgcc ggtgtcggtg 120

ccgaaaaaaa taccaatatt tgccataaca cacgctcctg ttgaaaaaga gatcccgcgg 180

ggaaaatgcg gtgaacatgt cagctattgc gaagagtgtg ccagttttgc tcacgggcaa 240

aagctgcacc agaatgggta ttaatgcacc agcctggcgc tttttttcgc ggcacgtccc 300

ctcgctaatg cccgtctggc gcggctttga cgctgataag gcgctgaata ccgatctgga 360

tcaaggtttt gtcgggttat cgtccaaaag gtgcactctt tgcatggtta taagtgcctg 420

acatggtgtc cgggcgaacg tcgccaggtg gcacaaattg tcagaactac gacacgacta 480

accgaccgca ggagtgtgcg atgaccctga atatgatgat ggatgccagc cgttctgtaa 540

taataaccgg acaattcggga ctgattaaaa aagcgccctc gcggcgcttt ttttatattc 600

tcgactccat ttaaaataaa aaatccaatc ggatttcact atttaaactg gccattatct 660

aagatgaatc cgatggaagc tcgctgtttt aacacgcgtt ttttaacctt ttattgaaag 720

tcggtgcttc tttgagcgaa cgatcaaatt taagtggatt cccatcaaaa aaatattctc 780

aacctaaaaa agtttgtgta atacttgtaa cgctacatgg agattaactc aatctagagg 840

gtattaataa tgaatcgtac taaactggta ctgggcgcaa ctcacttcac gccccgaagg 900

gggaagctgc ctgaccctac gattcccgct atttcattca ctgaccggag gttcaaaatg 960

acccagcgaa ccgagtcggg taataccgtc tggcgcttcg atttatccca gcagttcacc 1020

gcgatgcagc ggataagcgt ggttctcagc cgggcgaccg aggttgaaca gacactccag 1080

caggtgctgt gcgtattgca caatgacgcc tttttgcagc acggcatgat ctgtctgtac 1140

gacagccagc aggcgatttt gactattgaa gcgttgcagg aagccgatca gcagttgatc 1200

cccggcagct cgcaaattcg ctaccgtccg ggtgaagggc tggtcgggac ggtgctttcg 1260

caggggcaat cgttagtgct ggcgcgtgtg gctgacgatc agcgctttct tgaccgcctg 1320

ggactgtatg attacaacct gccgtttatc gccgtgccgc tgatagggcc ggatgcgcag 1380

acttttggcg tgctgacggc gcaaccgatg gcgcgttacg aagagcggtt acccgcctgc 1440

acccgctttc tggaaacggt c 1461

<210> 187

<211> 1185

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2

<400> 187

tccctgtgcg ccgcgtcgcc gatggtggcc agccaactgg cgcgctaccc gatcctgctc 60

gatgaactgc tcgacccgaa cacgctctat caaccgacgg cgatgaacgc ctatcgcgat 120

gaactgcgac aatacctgtt gcgcgtgccg gaagaggatg aagagcagca actggaggcg 180

ctacggcagt ttaagcaggc gcagttgttg cgcgtagcgg cggcggatat cgccggtacg 240

ttacccgtca tgaaagtgag cgatcactta acctggctgg cggaagcgat tatcgatgcg 300

gtggtgcagc aagcctggaa ccagatggtg gcgcgttacg gccagccgac gcatctgcac 360

gatcgcgaag ggcgcggttt cgccgtggtc ggttacggca aacttggcgg ctgggaatta 420

ggttacagct ccgatctgga tctggtgttc ctgcacgact gccccatgga tgtgatgacc 480

gatggcgagc gtgaaatcga tggccgccag ttctatttgc gcctcgcgca gcgcgtgatg 540

cacctgttca gcacgcgcac gtcgtccggc attctttatg aagtcgatgc gcgtttgcgc 600

660

aatgaagcct ggacatggga gcatcaggcg ctggcgcgtg cgcgcgtggt gtacggcgat 720

ccgcaactga ccgccgaatt tgacgccatt cgccgcgata tcctgatgac ctcccgcgat 780

gccgctaccc tgcaaaccga agtgcgggaa atgcgtgaga aaatgcgcgc ccatcttggt 840

aacaagcaca aagaccgttt cgatctgaaa gccgatgaag gcggtatcac cgatattgag 900

tttatcgctc agtatctggt gctgcgcttt gcccatgaga agccgaaact gacgcgctgg 960

tcggataatg tgcgcatcct cgaagggctg gcgcaaaacg gcatcatgga tgagcaggaa 1020

gcgcaggcat tgacgctggc gtacaccacg ttgcgtgatg agctgcacca cctggcgctg 1080

1140

accagctggg acaagtggct ggtggaaccg tgcgccccgg cgtaa 1185

<210> 188

<211> 2085

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2 with a 500 bp flanking region.

<400> 188

taaagcgagc gctcacttac gtgatctgtt gacgcagtcc gaagcgacca ttacttcagc 60

cgtttcagca gatacggcgg tgtggagtgc gcaatcagcc ctggcgaaac tggtgctcac 120

cgagtggtta gtgacgcagg gctggcgaac cttccttgat gaaaaagcgc aggctaagtt 180

tgccgactcc tttaaacgct ttgctgacgt tcatctgtca cgcagcgccg ccgagctgaa 240

aaaagccttt gcccagccgc tgggcgacag ctatcgcgac cagttaccgc ggctggcgcg 300

tgatatcgac agcgcgttat tgctggccgg acattacgat cgcgcgcgcg ccgtggagtg 360

gctggaaaac tggcaggggc ttcagcacgc tattgaaacg cgccagagag ttgaaatcga 420

acatttccgt aataccgcca ttacccagga gccgttctgg ttgcacagcg gaaaacgtta 480

acgaaaggat atttcgcatg tccctgtgcg ccgcgtcgcc gatggtggcc agccaactgg 540

cgcgctaccc gatcctgctc gatgaactgc tcgacccgaa cacgctctat caaccgacgg 600

cgatgaacgc ctatcgcgat gaactgcgac aatacctgtt gcgcgtgccg gaagaggatg 660

aagagcagca actggaggcg ctacggcagt ttaagcaggc gcagttgttg cgcgtagcgg 720

cggcggatat cgccggtacg ttacccgtca tgaaagtgag cgatcactta acctggctgg 780

840

gccagccgac gcatctgcac gatcgcgaag ggcgcggttt cgccgtggtc ggttacggca 900

aacttggcgg ctgggaatta ggttacagct ccgatctggga tctggtgttc ctgcacgact 960

gccccatgga tgtgatgacc gatggcgagc gtgaaatcga tggccgccag ttctatttgc 1020

gcctcgcgca gcgcgtgatg cacctgttca gcacgcgcac gtcgtccggc attctttatg 1080

aagtcgatgc gcgtttgcgc ccgtccggcg cggccggaat gctggtgacc actgcggaag 1140

cgttcgccga ttatcaaaaa aatgaagcct ggacatggga gcatcaggcg ctggcgcgtg 1200

cgcgcgtggt gtacggcgat ccgcaactga ccgccgaatt tgacgccatt cgccgcgata 1260

tcctgatgac ctcccgcgat gccgctaccc tgcaaaccga agtgcgggaa atgcgtgaga 1320

aaatgcgcgc ccatcttggt aacaagcaca aagaccgttt cgatctgaaa gccgatgaag 1380

gcggtatcac cgatattgag tttatcgctc agtatctggt gctgcgcttt gcccatgaga 1440

agccgaaact gacgcgctgg tcggataatg tgcgcatcct cgaagggctg gcgcaaaacg 1500

gcatcatgga tgagcaggaa gcgcaggcat tgacgctggc gtacaccacg ttgcgtgatg 1560

agctgcacca cctggcgctg caagagctgc caggacatgt ggcgctctcc tgttttgtcg 1620

ccgagcgtgc gcttatcaaa accagctggg acaagtggct ggtggaaccg tgcgccccgg 1680

cgtaagtgtg gtatcatcgc gcgcaaattt tgtatctctc aggagacagg aatgaaagtt 1740

acgctgccag agttcaatca agccggtgtc atggtggtgg gtgatgtgat gctggatcgc 1800

tactggtacg gcccaaccag ccgcatttct ccggaagcgc cagttccggt tgttaaagtc 1860

gatactattg aagagcgacc gggcggtgcg gcaaacgtgg cgatgaacat tgcctcgctg 1920

ggcgcaacgg cgcgtctggt tggcctgact ggcattgatg atgcggcgcg cgcgctgagc 1980

aaagcgctgg cggatgttaa tgttaaatgt gacttcgttt ctgttccgac tcaccccacc 2040

atcactaagc tgcgcgtgct gtcgcgtaac cagcaactga ttcgc 2085

<210> 189

<211> 452

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm7

<400> 189

atgaccctga atatgatgat ggatgccagc cgcgtcaggt tgaacgtaaa aaagtcggtc 60

tgcgcaaagc acgtcgtcgt ccgcagttct ccaaacgtta attggtttct gcttcggcag 120

aacgattggc gaaaaaaccc ggtgcgaacc gggttttttt atggataaag atcgtgttat 180

ccacagcaat ccattgatta tctcttcttt ttcagcattt ccagaatccc ctcaccacaa 240

agcccgcaaa atctggtaaa ctatcatcca attttctgcc caaatggctg ggattgttca 300

gccttacaac gagagtgaca gtacgcgcgg gtagttaact caacatctga 360

ccggtcgata actcacttca cgccccgaag ggggaagctg cctgacccta cgattcccgc 420

tattcattc actgaccgga ggttcaaaat ga 452

<210> 190

<211> 1451

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm7 with a 500 bp flanking region.

<400> 190

accggataag agagaaaagt gtcgacgtcg gtccggttga tattgaccgg cgcatccgcc 60

agctcgccca gtttttggtg gatctgtttg gcgattttgc gggtcttgcc ggtgtcggtg 120

ccgaaaaaaa taccaatatt tgccataaca cacgctcctg ttgaaaaaga gatcccgcgg 180

ggaaaatgcg gtgaacatgt cagctattgc gaagagtgtg ccagttttgc tcacgggcaa 240

aagctgcacc agaatgggta ttaatgcacc agcctggcgc tttttttcgc ggcacgtccc 300

ctcgctaatg cccgtctggc gcggctttga cgctgataag gcgctgaata ccgatctgga 360

tcaaggtttt gtcgggttat cgtccaaaag gtgcactctt tgcatggtta taagtgcctg 420

acatggtgtc cgggcgaacg tcgccaggtg gcacaaattg tcagaactac gacacgacta 480

accgaccgca ggagtgtgcg atgaccctga atatgatgat ggatgccagc cgcgtcaggt 540

tgaacgtaaa aaagtcggtc tgcgcaaagc acgtcgtcgt ccgcagttct ccaaacgtta 600

attggtttct gcttcggcag aacgattggc gaaaaaaccc ggtgcgaacc gggttttttt 660

atggataaag atcgtgttat ccacagcaat ccattgatta tctcttcttt ttcagcattt 720

ccagaatccc ctcaccacaa agcccgcaaa atctggtaaa ctatcatcca attttctgcc 780

caaatggctg ggattgttca ttttttgttt gccttacaac gagagtgaca gtacgcgcgg 840

gtagttaact caacatctga ccggtcgata actcacttca cgccccgaag ggggaagctg 900

cctgacccta cgattcccgc tatttcattc actgaccgga ggttcaaaat gacccagcga 960

accgagtcgg gtaataccgt ctggcgcttc gatttatccc agcagttcac cgcgatgcag 1020

cggataagcg tggttctcag ccgggcgacc gaggttgaac agacactcca gcaggtgctg 1080

tgcgtattgc acaatgacgc ctttttgcag cacggcatga tctgtctgta cgacagccag 1140

caggcgattt tgactattga agcgttgcag gaagccgatc agcagttgat ccccggcagc 1200

tcgcaaattc gctaccgtcc gggtgaaggg ctggtcggga cggtgctttc gcaggggcaa 1260

tcgttagtgc tggcgcgtgt ggctgacgat cagcgctttc ttgaccgcct gggactgtat 1320

gattacaacc tgccgtttat cgccgtgccg ctgatagggc cggatgcgca gacttttggc 1380

gtgctgacgg cgcaaccgat ggcgcgttac gaagagcggt tacccgcctg cacccgcttt 1440

ctggaaacgg t 1451

<210> 191

<211> 1191

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2

<400> 191

atggcgctga agcacctgat cacgctctgc gcggcgtcgc cgatggtcgc cagccagctg 60

gcgcgccacc cgctgctgct ggatgagctg ctggatccca acaccctcta tcagccgacg 120

gcgaccgatg cctatcgcga cgagctgcgc cagtacctgc tgcgcgtgcc ggaagaggat 180

gaagagcagc agctggaggc gttgcgccag tttaagcagg cgcagcagct gcatatcgcg 240

gcggcggata tcgctggtac cctgccggtg atgaaggtca gcgatcactt aacctggctt 300

gccgaagcga tcctcgacgc ggtggtgcag caggcatggg ggcagatggt cgctcgctac 360

ggccagccga cccacctgca cgatcgccag ggtcgcggct tcgccgtcgt cggctacggt 420

aagcttggcg gctgggagct gggctacagc tccgatctcg atctggtgtt cctccatgac 480

tgcccggcgg aggtgatgac cgacggcgag cgggagattg acggccgtca gttctacctg 540

cggctggccc agcggatcat gcacctgttc agcacccgca cctcgtccgg tattctctac 600

gaagtggacg cccggctgcg tccttctggc gcggcgggga tgctggtcac caccgccgac 660

gcgtttgctg actatcagca gaacgaagcc tggacgtggg aacatcaggc gctggtgcgc 720

gcccgcgtgg tctatggcga cccggcgctg caggcgcgct ttgacgccat tcgtcgcgat 780

atcctgacca ccccgcggga ggggatgacc ctgcagaccg aggttcgcga gatgcgcgag 840

aagatgcgcg cccaccttgg caacaaacat cccgatcgtt ttgatatcaa agccgatgcc 900

ggcgggatca ccgatattga atttattact cagtatctgg tcctacgcta tgccagtgac 960

aagccgaagc tgacccgctg gtctgacaac gtgcgtattc ttgagctgct ggcgcagaac 1020

gacatcatgg acgaggagga ggcgcgcgcc ttaacgcatg cgtacaccac cttgcgtgat 1080

gcgctccatc acctggccct gcaggagcag ccgggacacg tggcgccaga ggccttcagc 1140

cgggagcgtc agcaggtcag cgccagctgg cagaagtggc tgatggctta a 1191

<210> 192

<211> 2191

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2 with a 500 bp flanking region.

<400> 192

cgtaaggcga ccacccagct ccgcgcgttg ctgaacgacg ctgaagccgt tctgctggcc 60

gcggacaccg ccgacgaggc gttattccgc accgaggtcg tcggcgccaa actggccctg 120

actgaatggc tggtccagcg cggctggcgt ccgttcctca acgaggcagg agagaaaaaa 180

atagccggat cgttcaaacg gtttgccgat attaacctct cgcgggtggc ggccgagctg 240

cgcagcgccg tgcagcatct ggcggttgaa gatgccgccg accagttgcc gaagctgtcc 300

360

ctggagaact ggcaggagct tcaccgtgca atagcacatg acgatcgcag cgtctttgaa 420

tatttccgtc gccaggcgct ggctgccgag ccgttctggc tgcatagtgg aaaacgataa 480

tttcaggcca gggagccctt atggcgctga agcacctgat cacgctctgc gcggcgtcgc 540

cgatggtcgc cagccagctg gcgcgccacc cgctgctgct ggatgagctg ctggatccca 600

acaccctcta tcagccgacg gcgaccgatg cctatcgcga cgagctgcgc cagtacctgc 660

tgcgcgtgcc ggaagaggat gaagagcagc agctggaggc gttgcgccag tttaagcagg 720

cgcagcagct gcatatcgcg gcggcggata tcgctggtac cctgccggtg atgaaggtca 780

gcgatcactt aacctggctt gccgaagcga tcctcgacgc ggtggtgcag caggcatggg 840

ggcagatggt cgctcgctac ggccagccga cccacctgca cgatcgccag ggtcgcggct 900

tcgccgtcgt cggctacggt aagcttggcg gctgggagct gggctacagc tccgatctcg 960

atctggtgtt cctccatgac tgcccggcgg aggtgatgac cgacggcgag cgggagattg 1020

acggccgtca gttctacctg cggctggccc agcggatcat gcacctgttc agcacccgca 1080

cctcgtccgg tattctctac gaagtggacg cccggctgcg tccttctggc gcggcgggga 1140

tgctggtcac caccgccgac gcgtttgctg actatcagca gaacgaagcc tggacgtggg 1200

aacatcaggc gctggtgcgc gcccgcgtgg tctatggcga cccggcgctg caggcgcgct 1260

ttgacgccat tcgtcgcgat atcctgacca ccccgcggga ggggatgacc ctgcagaccg 1320

1380

ttgatatcaa agccgatgcc ggcgggatca ccgatattga atttattact cagtatctgg 1440

tcctacgcta tgccagtgac aagccgaagc tgacccgctg gtctgacaac gtgcgtattc 1500

ttgagctgct ggcgcagaac gacatcatgg acgaggagga ggcgcgcgcc ttaacgcatg 1560

cgtacaccac cttgcgtgat gcgctccatc acctggccct gcaggagcag ccgggacacg 1620

tggcgccaga ggccttcagc cgggagcgtc agcaggtcag cgccagctgg cagaagtggc 1680

tgatggctta actataaaat cgggtgtgct attatcgcgc gcaaagtttg cgtctcgcag 1740

1800

gatgtgatgc tggaccgcta ctggtacggc cccaccagtc gtatttcccc ggaagccccg 1860

gtgccggtgg tgaaggtgga aaatatcgaa gaacgtcctg gcggcgcggc aaacgtagcg 1920

atgaacatcg cctccctggg ggcaacgtcg cgcctggtgg gattgaccgg gattgatgac 1980

gctgcccgcg cgctgagcca ggcgctggcc aatgtgaatg tgaagtgcga cttcgtctcc 2040

gtcccgactc acccgaccat caccaagctg cgggtgctgt cgcgcaatca gcagctgatc 2100

cgcctcgact ttgaagaggg cttctccggc gtggatccgc agccgatgca tgagcgcatt 2160

cagcaggcgc tgggagccat tggcgcactg g 2191

<210> 193

<211> 613

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::PinfC

<400> 193

atgaccctga atatgatgct agaagcgtca ggtaccggtc atgattcacc gtgcgattct 60

cggttccctg gagcgcttca ttggcatcct gaccgaagag ttcgctggct tcttcccaac 120

ctggattgca ccagtgcagg tagtggtcat gaatattacc gattctcagg ctgaatacgt 180

taacgaattg acgcgtaaac tacaaaatgc gggcattcgt gtaaaagcag acttgagaaa 240

tgagaagatt ggctttaaaa tccgcgagca cactttacgt cgtgtcccgt atatgttggt 300

ctgtggcgac aaagaagtcg aagccggcaa agtggccgtg cgcacccgtc gcgggaaaga 360

cctcggcagc atggacgtaa gtgaagtgat tgagaagctg caacaagaga ttcgcagccg 420

cagtcttcaa caactggagg aataaggtat taaaggcgga aaacgagttc aaacggcacg 480

tccgaatcgt atcaatggcg agattcgcgc cctggaagtt cgcgccattg agctggcttc 540

ccgaccgcag ggcggcacct gcctgaccct gcgtttcccg ctgtttaaca ccctgaccgg 600

aggtgaagca tga 613

<210> 194

<211> 1613

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::PinfC with a 500 bp flanking region.

<400> 194

ggccgtcgcc cagcgtcggc gtccccaaca gcagggccgg gtaggccagc aggtccgcca 60

gcgtggcgcg gttaatattg accggggcgg cggcggcctc ccccagctgc ttgtggatca 120

ttttcgcgat cttgcggtt ttaccggtat cggtaccaaa gaaaatgcca atgttcgcca 180

tagtacgctc ctgtcggaat ggtgttgaaa aaaggaatga cgacagaggt attgcgaagg 240

ctgtgccagg ttgccctgca ccgcgacggc ccatccctgc cccatcagga tcgcttcgca 300

tcacgatgcc gcgcgccaaa ggcgcacccg gcggggcgaa aggtaaaaat ccgtgaattt 360

tccccctgtc ggatcaatgt ttcgcgtggt cgttccgata agggcgcaca ctttgcatgg 420

ttatccgggt tcggcttacc ccgccgcgtt ttgcgcacgg tgtcggacaa tttgtcataa 480

ctgcgacaca ggagtttgcg atgaccctga atatgatgct agaagcgtca ggtaccggtc 540

atgattcacc gtgcgattct cggttccctg gagcgcttca ttggcatcct gaccgaagag 600

ttcgctggct tcttcccaac ctggattgca ccagtgcagg tagtggtcat gaatattacc 660

gattctcagg ctgaatacgt taacgaattg acgcgtaaac tacaaaatgc gggcattcgt 720

gtaaaagcag acttgagaaa tgagaagatt ggctttaaaa tccgcgagca cactttacgt 780

cgtgtcccgt atatgttggt ctgtggcgac aaagaagtcg aagccggcaa agtggccgtg 840

cgcacccgtc gcgggaaaga cctcggcagc atggacgtaa gtgaagtgat tgagaagctg 900

caacaagaga ttcgcagccg cagtcttcaa caactggagg aataaggtat taaaggcgga 960

aaacgagttc aaacggcacg tccgaatcgt atcaatggcg agattcgcgc cctggaagtt 1020

1080 cgcgccattg agctggcttc ccgaccgcag ggcggcacct

ctgtttaaca ccctgaccgg aggtgaagca tgatccctga atccgacccg gacaccaccg 1140

tcagacgctt cgacctctct cagcagttca ccgccatgca gcggataagc gtggtgctga 1200

gccgggccac cgaggccagc aaaacgctgc aggaggtgct cagcgtatta cacaacgatg 1260

ccttttatgca gcacgggatg atctgcctgt acgacagcga gcaggagatc ctcagtatcg 1320

aagcgctgca gcaaaccggc cagcagcccc tccccggcag cacgcagatc cgctatcgcc 1380

ccggcgaggg actggtgggg accgtgctgg cccaggggca gtcgctggtg ctgccccggg 1440

tcgccgacga tcagcgtttt ctcgaccgcc tgagcctcta cgattacgat ctgccgttta 1500

tcgccgtacc gttgatgggg cccaacgccc ggccaatagg ggtgctggcg gcccagccga 1560

tggcgcgcca ggaagagcgg ctgccggcct gcacccgttt tctcgaaacc gtc 1613

<210> 195

<211> 1155

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 36 bp deletion glnE-delta-AR-2

<400> 195

atggcgctga agcacctgat cacgctctgc gcggcgtcgc cgatggtcgc cagccagctg 60

gcgcgccacc cgctgctgct ggatgagctg ctggatccca acaccctcta tcagccgacg 120

gcgaccgatg cctatcgcga cgagctgcgc cagtacctgc tgcgcgtgcc ggaagaggat 180

gaagagcagc agctgcatat cgcggcggcg gatatcgctg gtaccctgcc ggtgatgaag 240

gtcagcgatc acttaacctg gcttgccgaa gcgatcctcg acgcggtggt gcagcaggca 300

tgggggcaga tggtcgctcg ctacggccag ccgacccacc tgcacgatcg ccagggtcgc 360

ggcttcgccg tcgtcggcta cggtaagctt ggcggctggg agctgggcta cagctccgat 420

ctcgatctgg tgttcctcca tgactgcccg gcggaggtga tgaccgacgg cgagcgggag 480

attgacggcc gtcagttcta cctgcggctg gcccagcgga tcatgcacct gttcagcacc 540

600 cgcacctcgt ccggtattct ctacgaagtg

gggatgctgg tcaccaccgc cgacgcgttt gctgactatc agcagaacga agcctggacg 660

tgggaacatc aggcgctggt gcgcgcccgc gtggtctatg gcgacccggc gctgcaggcg 720

cgctttgacg ccattcgtcg cgatatcctg accaccccgc gggaggggat gaccctgcag 780

accgaggttc gcgagatgcg cgagaagatg cgcgcccacc ttggcaacaa acatcccgat 840

cgttttgata tcaaagccga tgccggcggg atcaccgata ttgaatttat tactcagtat 900

ctggtcctac gctatgccag tgacaagccg aagctgaccc gctggtctga caacgtgcgt 960

attcttgagc tgctggcgca gaacgacatc atggacgagg aggaggcgcg cgccttaacg 1020

catgcgtaca ccaccttgcg tgatgcgctc catcacctgg ccctgcagga gcagccggga 1080

cacgtggcgc cagaggcctt cagccgggag cgtcagcagg tcagcgccag ctggcagaag 1140

tggctgatgg cttaa 1155

<210> 196

<211> 2155

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 36 bp deletion glnE-delta-AR-2

<400> 196

cgtaaggcga ccacccagct ccgcgcgttg ctgaacgacg ctgaagccgt tctgctggcc 60

gcggacaccg ccgacgaggc gttattccgc accgaggtcg tcggcgccaa actggccctg 120

actgaatggc tggtccagcg cggctggcgt ccgttcctca acgaggcagg agagaaaaaa 180

atagccggat cgttcaaacg gtttgccgat attaacctct cgcgggtggc ggccgagctg 240

cgcagcgccg tgcagcatct ggcggttgaa gatgccgccg accagttgcc gaagctgtcc 300

360

ctggagaact ggcaggagct tcaccgtgca atagcacatg acgatcgcag cgtctttgaa 420

tatttccgtc gccaggcgct ggctgccgag ccgttctggc tgcatagtgg aaaacgataa 480

tttcaggcca gggagccctt atggcgctga agcacctgat cacgctctgc gcggcgtcgc 540

cgatggtcgc cagccagctg gcgcgccacc cgctgctgct ggatgagctg ctggatccca 600

acaccctcta tcagccgacg gcgaccgatg cctatcgcga cgagctgcgc cagtacctgc 660

tgcgcgtgcc ggaagaggat gaagagcagc agctgcatat cgcggcggcg gatatcgctg 720

gtaccctgcc ggtgatgaag gtcagcgatc acttaacctg gcttgccgaa gcgatcctcg 780

acgcggtggt gcagcaggca tgggggcaga tggtcgctcg ctacggccag ccgacccacc 840

tgcacgatcg ccagggtcgc ggcttcgccg tcgtcggcta cggtaagctt ggcggctggg 900

agctgggcta cagctccgat ctcgatctgg tgttcctcca tgactgcccg gcggaggtga 960

tgaccgacgg cgagcgggag attgacggcc gtcagttcta cctgcggctg gcccagcgga 1020

tcatgcacct gttcagcacc cgcacctcgt ccggtattct ctacgaagtg gacgcccggc 1080

tgcgtccttc tggcgcggcg gggatgctgg tcaccaccgc cgacgcgttt gctgactatc 1140

agcagaacga agcctggacg tgggaacatc aggcgctggt gcgcgcccgc gtggtctatg 1200

gcgacccggc gctgcaggcg cgctttgacg ccattcgtcg cgatatcctg accacccccgc 1260

gggaggggat gaccctgcag accgaggttc gcgagatgcg cgagaagatg cgcgcccacc 1320

1380

ttgaatttat tactcagtat ctggtcctac gctatgccag tgacaagccg aagctgaccc 1440

gctggtctga caacgtgcgt attcttgagc tgctggcgca gaacgacatc atggacgagg 1500

aggaggcgcg cgccttaacg catgcgtaca ccaccttgcg tgatgcgctc catcacctgg 1560

ccctgcagga gcagccggga cacgtggcgc cagaggcctt cagccggggag cgtcagcagg 1620

tcagcgccag ctggcagaag tggctgatgg cttaactata aaatcgggtg tgctattatc 1680

gcgcgcaaag tttgcgtctc gcaggagaga gtcatgaaag taacgctgcc ggagtttgaa 1740

cgtgcaggag tgttggtggt gggtgatgtg atgctggacc gctactggta cggccccacc 1800

1860

cctggcggcg cggcaaacgt agcgatgaac atcgcctccc tgggggcaac gtcgcgcctg 1920

gtgggattga ccgggattga tgacgctgcc cgcgcgctga gccaggcgct ggccaatgtg 1980

aatgtgaagt gcgacttcgt ctccgtcccg actcacccga ccatcaccaa gctgcgggtg 2040

ctgtcgcgca atcagcagct gatccgcctc gactttgaag agggcttctc cggcgtggat 2100

ccgcagccga tgcatgagcg cattcagcag gcgctgggag ccattggcgc actgg 2155

<210> 197

<211> 412

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm8.2

<400> 197

atgaccctga atatgatgct cgacgccgtc ctcgcagtac cattgcaacc gactttacag 60

caagaagtga ttctggcacg catggaacaa attcttgcca gtcgggcttt atccgatgac 120

gaacgcgcac agcttttata tgagcgcgga gtgttgtatg atagtctcgg tctgagggca 180

ttagcgcgaa atgatttttc acaagcgctg gcaatccgac ccgatatgcc tgaagtattc 240

aattacttag gcatttactt aacgcaggca ggcaattttg atgctgccta tgaagcgttt 300

gattctgtac ttgagcttga tcgccattga gctggcttcc cgaccgcagg gcggcacctg 360

cctgaccctg cgtttcccgc tgtttaacac cctgaccggga ggtgaagcat ga 412

<210> 198

<211> 1389

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm8.2 with a 500 bp flanking region.

<400> 198

cccaacagca gggccgggta ggccagcagg tccgccagcg tggcgcggtt aatattgacc 60

ggggcggcgg cggcctcccc cagctgcttg tggatcattt tcgcgatctt gcgggtttta 120

ccggtatcgg taccaaagaa aatgccaatg ttcgccatag tacgctcctg tcggaatggt 180

gttgaaaaaa ggaatgacga cagaggtatt gcgaaggctg tgccaggttg ccctgcaccg 240

cgacggccca tccctgcccc atcaggatcg cttcgcatca cgatgccgcg cgccaaaggc 300

360

gcgtggtcgt tccgataagg gcgcacactt tgcatggtta tcggggttcg gcttaccccg 420

ccgcgttttg cgcacggtgt cggacaattt gtcataactg cgacacaggga gtttgcgatg 480

accctgaata tgatgctcga cgccgtcctc gcagtaccat tgcaaccgac tttacagcaa 540

gaagtgattc tggcacgcat ggaacaaatt cttgccagtc gggctttatc cgatgacgaa 600

cgcgcacagc ttttatatga gcgcggagtg ttgtatgata gtctcggtct gagggcatta 660

gcgcgaaatg atttttcaca agcgctggca atccgacccg atatgcctga agtattcaat 720

tacttaggca tttacttaac gcaggcaggc aattttgatg ctgcctatga agcgtttgat 780

tctgtacttg agcttgatcg ccattgagct ggcttcccga ccgcaggggcg gcacctgcct 840

gaccctgcgt ttcccgctgt ttaacaccct gaccgggaggt gaagcatgat ccctgaatcc 900

gacccggaca ccaccgtcag acgcttcgac ctctctcagc agttcaccgc catgcagcgg 960

ataagcgtgg tgctgagccg ggccaccgag gccagcaaaa cgctgcagga ggtgctcagc 1020

gtattacaca acgatgcctt tatgcagcac gggatgatct gcctgtacga cagcgagcag 1080

gagatcctca gtatcgaagc gctgcagcaa accggccagc agcccctccc cggcagcacg 1140

cagatccgct atcgccccgg cgagggactg gtggggaccg tgctggccca ggggcagtcg 1200

ctggtgctgc cccgggtcgc cgacgatcag cgttttctcg accgcctgag cctctacgat 1260

tacgatctgc cgtttatcgc cgtaccgttg atggggccca acgcccggcc aataggggtg 1320

ctggcggccc agccgatggc gcgccaggaa gagcggctgc cggcctgcac ccgttttctc 1380

gaaaccgtc 1389

<210> 199

<211> 1155

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 36 bp deletion glnE-delta-AR-2

<400> 199

atggcgctga agcacctgat cacgctctgc gcggcgtcgc cgatggtcgc cagccagctg 60

gcgcgccacc cgctgctgct ggatgagctg ctggatccca acaccctcta tcagccgacg 120

gcgaccgatg cctatcgcga cgagctgcgc cagtacctgc tgcgcgtgcc ggaagaggat 180

gaagagcagc agctgcatat cgcggcggcg gatatcgctg gtaccctgcc ggtgatgaag 240

gtcagcgatc acttaacctg gcttgccgaa gcgatcctcg acgcggtggt gcagcaggca 300

tgggggcaga tggtcgctcg ctacggccag ccgacccacc tgcacgatcg ccagggtcgc 360

ggcttcgccg tcgtcggcta cggtaagctt ggcggctggg agctgggcta cagctccgat 420

ctcgatctgg tgttcctcca tgactgcccg gcggaggtga tgaccgacgg cgagcgggag 480

attgacggcc gtcagttcta cctgcggctg gcccagcgga tcatgcacct gttcagcacc 540

600 cgcacctcgt ccggtattct ctacgaagtg

gggatgctgg tcaccaccgc cgacgcgttt gctgactatc agcagaacga agcctggacg 660

tgggaacatc aggcgctggt gcgcgcccgc gtggtctatg gcgacccggc gctgcaggcg 720

cgctttgacg ccattcgtcg cgatatcctg accaccccgc gggaggggat gaccctgcag 780

accgaggttc gcgagatgcg cgagaagatg cgcgcccacc ttggcaacaa acatcccgat 840

cgttttgata tcaaagccga tgccggcggg atcaccgata ttgaatttat tactcagtat 900

ctggtcctac gctatgccag tgacaagccg aagctgaccc gctggtctga caacgtgcgt 960

attcttgagc tgctggcgca gaacgacatc atggacgagg aggaggcgcg cgccttaacg 1020

catgcgtaca ccaccttgcg tgatgcgctc catcacctgg ccctgcagga gcagccggga 1080

cacgtggcgc cagaggcctt cagccgggag cgtcagcagg tcagcgccag ctggcagaag 1140

tggctgatgg cttaa 1155

<210> 200

<211> 2155

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 36 bp deletion glnE-delta-AR-2

<400> 200

cgtaaggcga ccacccagct ccgcgcgttg ctgaacgacg ctgaagccgt tctgctggcc 60

gcggacaccg ccgacgaggc gttattccgc accgaggtcg tcggcgccaa actggccctg 120

actgaatggc tggtccagcg cggctggcgt ccgttcctca acgaggcagg agagaaaaaa 180

atagccggat cgttcaaacg gtttgccgat attaacctct cgcgggtggc ggccgagctg 240

cgcagcgccg tgcagcatct ggcggttgaa gatgccgccg accagttgcc gaagctgtcc 300

360

ctggagaact ggcaggagct tcaccgtgca atagcacatg acgatcgcag cgtctttgaa 420

tatttccgtc gccaggcgct ggctgccgag ccgttctggc tgcatagtgg aaaacgataa 480

tttcaggcca gggagccctt atggcgctga agcacctgat cacgctctgc gcggcgtcgc 540

cgatggtcgc cagccagctg gcgcgccacc cgctgctgct ggatgagctg ctggatccca 600

acaccctcta tcagccgacg gcgaccgatg cctatcgcga cgagctgcgc cagtacctgc 660

tgcgcgtgcc ggaagaggat gaagagcagc agctgcatat cgcggcggcg gatatcgctg 720

gtaccctgcc ggtgatgaag gtcagcgatc acttaacctg gcttgccgaa gcgatcctcg 780

acgcggtggt gcagcaggca tgggggcaga tggtcgctcg ctacggccag ccgacccacc 840

tgcacgatcg ccagggtcgc ggcttcgccg tcgtcggcta cggtaagctt ggcggctggg 900

agctgggcta cagctccgat ctcgatctgg tgttcctcca tgactgcccg gcggaggtga 960

tgaccgacgg cgagcgggag attgacggcc gtcagttcta cctgcggctg gcccagcgga 1020

tcatgcacct gttcagcacc cgcacctcgt ccggtattct ctacgaagtg gacgcccggc 1080

tgcgtccttc tggcgcggcg gggatgctgg tcaccaccgc cgacgcgttt gctgactatc 1140

agcagaacga agcctggacg tgggaacatc aggcgctggt gcgcgcccgc gtggtctatg 1200

gcgacccggc gctgcaggcg cgctttgacg ccattcgtcg cgatatcctg accacccccgc 1260

gggaggggat gaccctgcag accgaggttc gcgagatgcg cgagaagatg cgcgcccacc 1320

1380

ttgaatttat tactcagtat ctggtcctac gctatgccag tgacaagccg aagctgaccc 1440

gctggtctga caacgtgcgt attcttgagc tgctggcgca gaacgacatc atggacgagg 1500

aggaggcgcg cgccttaacg catgcgtaca ccaccttgcg tgatgcgctc catcacctgg 1560

ccctgcagga gcagccggga cacgtggcgc cagaggcctt cagccggggag cgtcagcagg 1620

tcagcgccag ctggcagaag tggctgatgg cttaactata aaatcgggtg tgctattatc 1680

gcgcgcaaag tttgcgtctc gcaggagaga gtcatgaaag taacgctgcc ggagtttgaa 1740

cgtgcaggag tgttggtggt gggtgatgtg atgctggacc gctactggta cggccccacc 1800

1860

cctggcggcg cggcaaacgt agcgatgaac atcgcctccc tgggggcaac gtcgcgcctg 1920

gtgggattga ccgggattga tgacgctgcc cgcgcgctga gccaggcgct ggccaatgtg 1980

aatgtgaagt gcgacttcgt ctccgtcccg actcacccga ccatcaccaa gctgcgggtg 2040

ctgtcgcgca atcagcagct gatccgcctc gactttgaag agggcttctc cggcgtggat 2100

ccgcagccga tgcatgagcg cattcagcag gcgctgggag ccattggcgc actgg 2155

<210> 201

<211> 413

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm6.2

<400> 201

atgaccctga atatgatgct cgagctaaag ttctcggcta atcgctgata acatttgacg 60

caatgcgcaa taaaagggca tcatttgatg ccctttttgc acgctttcat accagaacct 120

ggctcatcag tgattttttt tgtcataatc attgctgaga caggctctga agagggcgtt 180

tatacaccaa accattcgag cggtagcgcg acggcaagtc agcgttctcc tttgcaatag 240

cagggaagag gcgccagaac cgccagcgtt gaagcagttt gaacgcgttc agtgtataat 300

ccgaaactta atttcggttt ggagccattg agctggcttc ccgaccgcag ggcggcacct 360

gcctgaccct gcgtttcccg ctgtttaaca ccctgaccgg aggtgaagca tga 413

<210> 202

<211> 1413

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm6.2 with a 500 bp flanking region.

<400> 202

ggccgtcgcc cagcgtcggc gtccccaaca gcagggccgg gtaggccagc aggtccgcca 60

gcgtggcgcg gttaatattg accggggcgg cggcggcctc ccccagctgc ttgtggatca 120

ttttcgcgat cttgcggtt ttaccggtat cggtaccaaa gaaaatgcca atgttcgcca 180

tagtacgctc ctgtcggaat ggtgttgaaa aaaggaatga cgacagaggt attgcgaagg 240

ctgtgccagg ttgccctgca ccgcgacggc ccatccctgc cccatcagga tcgcttcgca 300

tcacgatgcc gcgcgccaaa ggcgcacccg gcggggcgaa aggtaaaaat ccgtgaattt 360

tccccctgtc ggatcaatgt ttcgcgtggt cgttccgata agggcgcaca ctttgcatgg 420

ttatccgggt tcggcttacc ccgccgcgtt ttgcgcacgg tgtcggacaa tttgtcataa 480

ctgcgacaca ggagtttgcg atgaccctga atatgatgct cgagctaaag ttctcggcta 540

atcgctgata acatttgacg caatgcgcaa taaaagggca tcatttgatg ccctttttgc 600

acgctttcat accagaacct ggctcatcag tgattttttt tgtcataatc attgctgaga 660

caggctctga agagggcgtt tatacaccaa accattcgag cggtagcgcg acggcaagtc 720

agcgttctcc tttgcaatag cagggaagag gcgccagaac cgccagcgtt gaagcagttt 780

gaacgcgttc agtgtataat ccgaaactta atttcggttt ggagccattg agctggcttc 840

ccgaccgcag ggcggcacct gcctgaccct gcgtttcccg ctgtttaaca ccctgaccgg 900

aggtgaagca tgatccctga atccgacccg gacaccaccg tcagacgctt cgacctctct 960

cagcagttca ccgccatgca gcggataagc gtggtgctga gccgggccac cgaggccagc 1020

aaaacgctgc aggaggtgct cagcgtatta cacaacgatg cctttatgca gcacgggatg 1080

atctgcctgt acgacagcga gcaggagatc ctcagtatcg aagcgctgca gcaaaccggc 1140

cagcagcccc tccccggcag cacgcagatc cgctatcgcc ccggcgaggg actggtgggg 1200

accgtgctgg cccaggggca gtcgctggtg ctgccccggg tcgccgacga tcagcgtttt 1260

ctcgaccgcc tgagcctcta cgattacgat ctgccgttta tcgccgtacc gttgatgggg 1320

cccaacgccc ggccaatagg ggtgctggcg gcccagccga tggcgcgcca ggaagagcgg 1380

ctgccggcct gcacccgttt tctcgaaacc gtc 1413

<210> 203

<211> 513

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1.2

<400> 203

atgaccctga atatgatgct cgagcccgct gaccgaccag aacttccacc ttggactcgg 60

ctataccctt ggcgtgacgg cgcgcgataa ctgggactac atccccattc cggtgatctt 120

accattggcg tcaataggtt acggtccggc gactttccag atgacctata ttcccggcac 180

ctacaataac ggtaacgttt acttcgcctg ggctcgtata cagttttaat tcgctaagtc 240

ttagcaataa atgagataag cggtgtgtct tgtggaaaaa caaggactaa agcgttaccc 300

actaaaaaag atagcgactt ttatcacttt ttagcaaagt tgcactggac aaaaggtacc 360

acaattggtg tactgatact cgacacagca ttagtgtcga tttttcatat aaaggtaatt 420

ttggccattg agctggcttc ccgaccgcag ggcggcacct gcctgaccct gcgtttcccg 480

ctgtttaaca ccctgaccgg aggtgaagca tga 513

<210> 204

<211> 1513

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1.2 with a 500 bp flanking region.

<400> 204

ggccgtcgcc cagcgtcggc gtccccaaca gcagggccgg gtaggccagc aggtccgcca 60

gcgtggcgcg gttaatattg accggggcgg cggcggcctc ccccagctgc ttgtggatca 120

ttttcgcgat cttgcggtt ttaccggtat cggtaccaaa gaaaatgcca atgttcgcca 180

tagtacgctc ctgtcggaat ggtgttgaaa aaaggaatga cgacagaggt attgcgaagg 240

ctgtgccagg ttgccctgca ccgcgacggc ccatccctgc cccatcagga tcgcttcgca 300

tcacgatgcc gcgcgccaaa ggcgcacccg gcggggcgaa aggtaaaaat ccgtgaattt 360

tccccctgtc ggatcaatgt ttcgcgtggt cgttccgata agggcgcaca ctttgcatgg 420

ttatccgggt tcggcttacc ccgccgcgtt ttgcgcacgg tgtcggacaa tttgtcataa 480

ctgcgacaca ggagtttgcg atgaccctga atatgatgct cgagcccgct gaccgaccag 540

aacttccacc ttggactcgg ctataccctt ggcgtgacgg cgcgcgataa ctgggactac 600

atccccattc cggtgatctt accattggcg tcaataggtt acggtccggc gactttccag 660

atgacctata ttcccggcac ctacaataac ggtaacgttt acttcgcctg ggctcgtata 720

cagttttaat tcgctaagtc ttagcaataa atgagataag cggtgtgtct tgtggaaaaa 780

caaggactaa agcgttaccc actaaaaaag atagcgactt ttatcacttt ttagcaaagt 840

tgcactggac aaaaggtacc acaattggtg tactgatact cgacacagca ttagtgtcga 900

tttttcatat aaaggtaatt ttggccattg agctggcttc ccgaccgcag ggcggcacct 960

gcctgaccct gcgtttcccg ctgtttaaca ccctgaccgg aggtgaagca tgatccctga 1020

atccgacccg gacaccaccg tcagacgctt cgacctctct cagcagttca ccgccatgca 1080

gcggataagc gtggtgctga gccgggccac cgaggccagc aaaacgctgc aggaggtgct 1140

cagcgtatta cacaacgatg cctttatgca gcacgggatg atctgcctgt acgacagcga 1200

gcaggagatc ctcagtatcg aagcgctgca gcaaaccggc cagcagcccc tccccggcag 1260

cacgcagatc cgctatcgcc ccggcgaggg actggtgggg accgtgctgg cccaggggca 1320

gtcgctggtg ctgccccggg tcgccgacga tcagcgtttt ctcgaccgcc tgagcctcta 1380

cgattacgat ctgccgttta tcgccgtacc gttgatgggg cccaacgccc ggccaatagg 1440

ggtgctggcg gcccagccga tggcgcgcca ggaagagcgg ctgccggcct gcacccgttt 1500

tctcgaaacc gtc 1513

<210> 205

<211> 1155

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 36 bp deletion glnE-delta-AR-2

<400> 205

atggcgctga agcacctgat cacgctctgc gcggcgtcgc cgatggtcgc cagccagctg 60

gcgcgccacc cgctgctgct ggatgagctg ctggatccca acaccctcta tcagccgacg 120

gcgaccgatg cctatcgcga cgagctgcgc cagtacctgc tgcgcgtgcc ggaagaggat 180

gaagagcagc agctgcatat cgcggcggcg gatatcgctg gtaccctgcc ggtgatgaag 240

gtcagcgatc acttaacctg gcttgccgaa gcgatcctcg acgcggtggt gcagcaggca 300

tgggggcaga tggtcgctcg ctacggccag ccgacccacc tgcacgatcg ccagggtcgc 360

ggcttcgccg tcgtcggcta cggtaagctt ggcggctggg agctgggcta cagctccgat 420

ctcgatctgg tgttcctcca tgactgcccg gcggaggtga tgaccgacgg cgagcgggag 480

attgacggcc gtcagttcta cctgcggctg gcccagcgga tcatgcacct gttcagcacc 540

600 cgcacctcgt ccggtattct ctacgaagtg

gggatgctgg tcaccaccgc cgacgcgttt gctgactatc agcagaacga agcctggacg 660

tgggaacatc aggcgctggt gcgcgcccgc gtggtctatg gcgacccggc gctgcaggcg 720

cgctttgacg ccattcgtcg cgatatcctg accaccccgc gggaggggat gaccctgcag 780

accgaggttc gcgagatgcg cgagaagatg cgcgcccacc ttggcaacaa acatcccgat 840

cgttttgata tcaaagccga tgccggcggg atcaccgata ttgaatttat tactcagtat 900

ctggtcctac gctatgccag tgacaagccg aagctgaccc gctggtctga caacgtgcgt 960

attcttgagc tgctggcgca gaacgacatc atggacgagg aggaggcgcg cgccttaacg 1020

catgcgtaca ccaccttgcg tgatgcgctc catcacctgg ccctgcagga gcagccggga 1080

cacgtggcgc cagaggcctt cagccgggag cgtcagcagg tcagcgccag ctggcagaag 1140

tggctgatgg cttaa 1155

<210> 206

<211> 2155

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 36 bp deletion glnE-delta-AR-2

<400> 206

cgtaaggcga ccacccagct ccgcgcgttg ctgaacgacg ctgaagccgt tctgctggcc 60

gcggacaccg ccgacgaggc gttattccgc accgaggtcg tcggcgccaa actggccctg 120

actgaatggc tggtccagcg cggctggcgt ccgttcctca acgaggcagg agagaaaaaa 180

atagccggat cgttcaaacg gtttgccgat attaacctct cgcgggtggc ggccgagctg 240

cgcagcgccg tgcagcatct ggcggttgaa gatgccgccg accagttgcc gaagctgtcc 300

360

ctggagaact ggcaggagct tcaccgtgca atagcacatg acgatcgcag cgtctttgaa 420

tatttccgtc gccaggcgct ggctgccgag ccgttctggc tgcatagtgg aaaacgataa 480

tttcaggcca gggagccctt atggcgctga agcacctgat cacgctctgc gcggcgtcgc 540

cgatggtcgc cagccagctg gcgcgccacc cgctgctgct ggatgagctg ctggatccca 600

acaccctcta tcagccgacg gcgaccgatg cctatcgcga cgagctgcgc cagtacctgc 660

tgcgcgtgcc ggaagaggat gaagagcagc agctgcatat cgcggcggcg gatatcgctg 720

gtaccctgcc ggtgatgaag gtcagcgatc acttaacctg gcttgccgaa gcgatcctcg 780

acgcggtggt gcagcaggca tgggggcaga tggtcgctcg ctacggccag ccgacccacc 840

tgcacgatcg ccagggtcgc ggcttcgccg tcgtcggcta cggtaagctt ggcggctggg 900

agctgggcta cagctccgat ctcgatctgg tgttcctcca tgactgcccg gcggaggtga 960

tgaccgacgg cgagcgggag attgacggcc gtcagttcta cctgcggctg gcccagcgga 1020

tcatgcacct gttcagcacc cgcacctcgt ccggtattct ctacgaagtg gacgcccggc 1080

tgcgtccttc tggcgcggcg gggatgctgg tcaccaccgc cgacgcgttt gctgactatc 1140

agcagaacga agcctggacg tgggaacatc aggcgctggt gcgcgcccgc gtggtctatg 1200

gcgacccggc gctgcaggcg cgctttgacg ccattcgtcg cgatatcctg accacccccgc 1260

gggaggggat gaccctgcag accgaggttc gcgagatgcg cgagaagatg cgcgcccacc 1320

1380

ttgaatttat tactcagtat ctggtcctac gctatgccag tgacaagccg aagctgaccc 1440

gctggtctga caacgtgcgt attcttgagc tgctggcgca gaacgacatc atggacgagg 1500

aggaggcgcg cgccttaacg catgcgtaca ccaccttgcg tgatgcgctc catcacctgg 1560

ccctgcagga gcagccggga cacgtggcgc cagaggcctt cagccggggag cgtcagcagg 1620

tcagcgccag ctggcagaag tggctgatgg cttaactata aaatcgggtg tgctattatc 1680

gcgcgcaaag tttgcgtctc gcaggagaga gtcatgaaag taacgctgcc ggagtttgaa 1740

cgtgcaggag tgttggtggt gggtgatgtg atgctggacc gctactggta cggccccacc 1800

1860

cctggcggcg cggcaaacgt agcgatgaac atcgcctccc tgggggcaac gtcgcgcctg 1920

gtgggattga ccgggattga tgacgctgcc cgcgcgctga gccaggcgct ggccaatgtg 1980

aatgtgaagt gcgacttcgt ctccgtcccg actcacccga ccatcaccaa gctgcgggtg 2040

ctgtcgcgca atcagcagct gatccgcctc gactttgaag agggcttctc cggcgtggat 2100

ccgcagccga tgcatgagcg cattcagcag gcgctgggag ccattggcgc actgg 2155

<210> 207

<211> 613

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::PinfC

<400> 207

atgaccctga atatgatgct agaagcgtca ggtaccggtc atgattcacc gtgcgattct 60

cggttccctg gagcgcttca ttggcatcct gaccgaagag ttcgctggct tcttcccaac 120

ctggattgca ccagtgcagg tagtggtcat gaatattacc gattctcagg ctgaatacgt 180

taacgaattg acgcgtaaac tacaaaatgc gggcattcgt gtaaaagcag acttgagaaa 240

tgagaagatt ggctttaaaa tccgcgagca cactttacgt cgtgtcccgt atatgttggt 300

ctgtggcgac aaagaagtcg aagccggcaa agtggccgtg cgcacccgtc gcgggaaaga 360

cctcggcagc atggacgtaa gtgaagtgat tgagaagctg caacaagaga ttcgcagccg 420

cagtcttcaa caactggagg aataaggtat taaaggcgga aaacgagttc aaacggcacg 480

tccgaatcgt atcaatggcg agattcgcgc cctggaagtt cgcgccattg agctggcttc 540

ccgaccgcag ggcggcacct gcctgaccct gcgtttcccg ctgtttaaca ccctgaccgg 600

aggtgaagca tga 613

<210> 208

<211> 1613

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::PinfC with a 500 bp flanking region.

<400> 208

ggccgtcgcc cagcgtcggc gtccccaaca gcagggccgg gtaggccagc aggtccgcca 60

gcgtggcgcg gttaatattg accggggcgg cggcggcctc ccccagctgc ttgtggatca 120

ttttcgcgat cttgcggtt ttaccggtat cggtaccaaa gaaaatgcca atgttcgcca 180

tagtacgctc ctgtcggaat ggtgttgaaa aaaggaatga cgacagaggt attgcgaagg 240

ctgtgccagg ttgccctgca ccgcgacggc ccatccctgc cccatcagga tcgcttcgca 300

tcacgatgcc gcgcgccaaa ggcgcacccg gcggggcgaa aggtaaaaat ccgtgaattt 360

tccccctgtc ggatcaatgt ttcgcgtggt cgttccgata agggcgcaca ctttgcatgg 420

ttatccgggt tcggcttacc ccgccgcgtt ttgcgcacgg tgtcggacaa tttgtcataa 480

ctgcgacaca ggagtttgcg atgaccctga atatgatgct agaagcgtca ggtaccggtc 540

atgattcacc gtgcgattct cggttccctg gagcgcttca ttggcatcct gaccgaagag 600

ttcgctggct tcttcccaac ctggattgca ccagtgcagg tagtggtcat gaatattacc 660

gattctcagg ctgaatacgt taacgaattg acgcgtaaac tacaaaatgc gggcattcgt 720

gtaaaagcag acttgagaaa tgagaagatt ggctttaaaa tccgcgagca cactttacgt 780

cgtgtcccgt atatgttggt ctgtggcgac aaagaagtcg aagccggcaa agtggccgtg 840

cgcacccgtc gcgggaaaga cctcggcagc atggacgtaa gtgaagtgat tgagaagctg 900

caacaagaga ttcgcagccg cagtcttcaa caactggagg aataaggtat taaaggcgga 960

aaacgagttc aaacggcacg tccgaatcgt atcaatggcg agattcgcgc cctggaagtt 1020

1080 cgcgccattg agctggcttc ccgaccgcag ggcggcacct

ctgtttaaca ccctgaccgg aggtgaagca tgatccctga atccgacccg gacaccaccg 1140

tcagacgctt cgacctctct cagcagttca ccgccatgca gcggataagc gtggtgctga 1200

gccgggccac cgaggccagc aaaacgctgc aggaggtgct cagcgtatta cacaacgatg 1260

ccttttatgca gcacgggatg atctgcctgt acgacagcga gcaggagatc ctcagtatcg 1320

aagcgctgca gcaaaccggc cagcagcccc tccccggcag cacgcagatc cgctatcgcc 1380

ccggcgaggg actggtgggg accgtgctgg cccaggggca gtcgctggtg ctgccccggg 1440

tcgccgacga tcagcgtttt ctcgaccgcc tgagcctcta cgattacgat ctgccgttta 1500

tcgccgtacc gttgatgggg cccaacgccc ggccaatagg ggtgctggcg gcccagccga 1560

tggcgcgcca ggaagagcgg ctgccggcct gcacccgttt tctcgaaacc gtc 1613

<210> 209

<211> 1191

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2

<400> 209

atggcgctga agcacctgat cacgctctgc gcggcgtcgc cgatggtcgc cagccagctg 60

gcgcgccacc cgctgctgct ggatgagctg ctggatccca acaccctcta tcagccgacg 120

gcgaccgatg cctatcgcga cgagctgcgc cagtacctgc tgcgcgtgcc ggaagaggat 180

gaagagcagc agctggaggc gttgcgccag tttaagcagg cgcagcagct gcatatcgcg 240

gcggcggata tcgctggtac cctgccggtg atgaaggtca gcgatcactt aacctggctt 300

gccgaagcga tcctcgacgc ggtggtgcag caggcatggg ggcagatggt cgctcgctac 360

ggccagccga cccacctgca cgatcgccag ggtcgcggct tcgccgtcgt cggctacggt 420

aagcttggcg gctgggagct gggctacagc tccgatctcg atctggtgtt cctccatgac 480

tgcccggcgg aggtgatgac cgacggcgag cgggagattg acggccgtca gttctacctg 540

cggctggccc agcggatcat gcacctgttc agcacccgca cctcgtccgg tattctctac 600

gaagtggacg cccggctgcg tccttctggc gcggcgggga tgctggtcac caccgccgac 660

gcgtttgctg actatcagca gaacgaagcc tggacgtggg aacatcaggc gctggtgcgc 720

gcccgcgtgg tctatggcga cccggcgctg caggcgcgct ttgacgccat tcgtcgcgat 780

atcctgacca ccccgcggga ggggatgacc ctgcagaccg aggttcgcga gatgcgcgag 840

aagatgcgcg cccaccttgg caacaaacat cccgatcgtt ttgatatcaa agccgatgcc 900

ggcgggatca ccgatattga atttattact cagtatctgg tcctacgcta tgccagtgac 960

aagccgaagc tgacccgctg gtctgacaac gtgcgtattc ttgagctgct ggcgcagaac 1020

gacatcatgg acgaggagga ggcgcgcgcc ttaacgcatg cgtacaccac cttgcgtgat 1080

gcgctccatc acctggccct gcaggagcag ccgggacacg tggcgccaga ggccttcagc 1140

cgggagcgtc agcaggtcag cgccagctgg cagaagtggc tgatggctta a 1191

<210> 210

<211> 2191

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2 with a 500 bp flanking region.

<400> 210

cgtaaggcga ccacccagct ccgcgcgttg ctgaacgacg ctgaagccgt tctgctggcc 60

gcggacaccg ccgacgaggc gttattccgc accgaggtcg tcggcgccaa actggccctg 120

actgaatggc tggtccagcg cggctggcgt ccgttcctca acgaggcagg agagaaaaaa 180

atagccggat cgttcaaacg gtttgccgat attaacctct cgcgggtggc ggccgagctg 240

cgcagcgccg tgcagcatct ggcggttgaa gatgccgccg accagttgcc gaagctgtcc 300

360

ctggagaact ggcaggagct tcaccgtgca atagcacatg acgatcgcag cgtctttgaa 420

tatttccgtc gccaggcgct ggctgccgag ccgttctggc tgcatagtgg aaaacgataa 480

tttcaggcca gggagccctt atggcgctga agcacctgat cacgctctgc gcggcgtcgc 540

cgatggtcgc cagccagctg gcgcgccacc cgctgctgct ggatgagctg ctggatccca 600

acaccctcta tcagccgacg gcgaccgatg cctatcgcga cgagctgcgc cagtacctgc 660

tgcgcgtgcc ggaagaggat gaagagcagc agctggaggc gttgcgccag tttaagcagg 720

cgcagcagct gcatatcgcg gcggcggata tcgctggtac cctgccggtg atgaaggtca 780

gcgatcactt aacctggctt gccgaagcga tcctcgacgc ggtggtgcag caggcatggg 840

ggcagatggt cgctcgctac ggccagccga cccacctgca cgatcgccag ggtcgcggct 900

tcgccgtcgt cggctacggt aagcttggcg gctgggagct gggctacagc tccgatctcg 960

atctggtgtt cctccatgac tgcccggcgg aggtgatgac cgacggcgag cgggagattg 1020

acggccgtca gttctacctg cggctggccc agcggatcat gcacctgttc agcacccgca 1080

cctcgtccgg tattctctac gaagtggacg cccggctgcg tccttctggc gcggcgggga 1140

tgctggtcac caccgccgac gcgtttgctg actatcagca gaacgaagcc tggacgtggg 1200

aacatcaggc gctggtgcgc gcccgcgtgg tctatggcga cccggcgctg caggcgcgct 1260

ttgacgccat tcgtcgcgat atcctgacca ccccgcggga ggggatgacc ctgcagaccg 1320

1380

ttgatatcaa agccgatgcc ggcgggatca ccgatattga atttattact cagtatctgg 1440

tcctacgcta tgccagtgac aagccgaagc tgacccgctg gtctgacaac gtgcgtattc 1500

ttgagctgct ggcgcagaac gacatcatgg acgaggagga ggcgcgcgcc ttaacgcatg 1560

cgtacaccac cttgcgtgat gcgctccatc acctggccct gcaggagcag ccgggacacg 1620

tggcgccaga ggccttcagc cgggagcgtc agcaggtcag cgccagctgg cagaagtggc 1680

tgatggctta actataaaat cgggtgtgct attatcgcgc gcaaagtttg cgtctcgcag 1740

1800

gatgtgatgc tggaccgcta ctggtacggc cccaccagtc gtatttcccc ggaagccccg 1860

gtgccggtgg tgaaggtgga aaatatcgaa gaacgtcctg gcggcgcggc aaacgtagcg 1920

atgaacatcg cctccctggg ggcaacgtcg cgcctggtgg gattgaccgg gattgatgac 1980

gctgcccgcg cgctgagcca ggcgctggcc aatgtgaatg tgaagtgcga cttcgtctcc 2040

gtcccgactc acccgaccat caccaagctg cgggtgctgt cgcgcaatca gcagctgatc 2100

cgcctcgact ttgaagaggg cttctccggc gtggatccgc agccgatgca tgagcgcatt 2160

cagcaggcgc tgggagccat tggcgcactg g 2191

<210> 211

<211> 1188

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2

<400> 211

atggcgctca aacagttaat ccgtctgtgt gccgcctcgc cgatggtcgc gacacaactt 60

gcacgtcatc ctttattgct cgatgaactg ctcgacccgc gcacgcttta ccagccgatt 120

gagccgggcg cttaccgcga cgaactgcgt cagtatctga tgcgggtgcc aacagaagac 180

gaagaacagc agcttgaagc cgtgcgccag ttcaaacagg cccagcattt gcgtatcgca 240

gccggggata tttccggggc attgccggtg atgaaagtca gtgaccattt aacctacctt 300

gccgaggcca ttctcgatgt cgtggtgcag catgcgtggg aacaaatggt cgtaaaatac 360

gggcagcccg cgcatcttca gcaccgtgag gggcgcggtt ttgccgtggt cggttacggg 420

aaactcggtg gctgggagct gggttatagc tcagatctgg atctggtctt cctgctcgat 480

tgcgcgccgg aggtgatgac ggacggcgaa cgcagcatcg acggacgtca gttttatctt 540

cggctggcgc agcgcattat gcacttattc agcaccggga catcgtcagg cattctttac 600

gaggttgatc cgcgtctgcg accttccggc gcatccggca tgctggtcag taccattgaa 660

gcgtttgcag attatcaggc caatgaagcc tggacgtggg agcatcaggc gctggttcgc 720

gcgcgcgtgg tttacgggga tccgcaactg acacagcaat ttaacgccac gcgtcgcgac 780

attctttgcc gccagcgcga tggcgacggc ctgcgtaagg aggtccgtga aatgcgcgag 840

aaaatgtatg cccatctggg gagtaaaaaa gcccacgagt ttgatctgaa agccgatccg 900

ggtggcatca cggatattga attcattgca caatacctgg ttctgcgttt cgcgcatgat 960

gagccgaagc tgacgcgctg gtctgataac gtgcggattt ttgaactgat ggcacgatat 1020

gacatcatgc cggaagagga agcgcgccat ctgacgcagg cttatgtgac gctgcgcgat 1080

gaaattcatc atctggcgtt gcaggaacac agcgggaaag tggccgcgga cagctttgct 1140

actgagcgcg cgcagatccg tgccagctgg gcaaagtggc tcggctga 1188

<210> 212

<211> 2188

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2 with a 500 bp flanking region.

<400> 212

cggtactgga acagaaatcg gcggatgcgc aggaaatttg ttatgacacg gcctgtctga 60

agtgcaagtt agtgcttact tcctggctgg caacctcagg ctggacgccg tttattgatg 120

ataaatctgc gaagaaactg gacgcttcct tcaaacgttt tgctgacatc atgctcggtc 180

gtaccgcagc ggatctgaaa gaagcctttg cgcagccact gacggaagaa ggttatcgcg 240

atcagctggc gcgcctgaaa cgccagatca ttaccttcca tttgcttgcc ggtgcttacc 300

ctgaaaaaga cgtcgatgcg tatattgccg gctgggtgga cctgcaacag gccatcgttc 360

agcagcaaca cgcctgggag gattcggccc gttctcacgc ggtgatgatg gatgctttct 420

ggttaaacgg gcaacctcgt taactgactg actagcctgg gcaaactgcc cgggcttttt 480

tttgcaagga atctgatttc atggcgctca aacagttaat ccgtctgtgt gccgcctcgc 540

cgatggtcgc gacacaactt gcacgtcatc ctttattgct cgatgaactg ctcgacccgc 600

gcacgcttta ccagccgatt gagccgggcg cttaccgcga cgaactgcgt cagtatctga 660

tgcgggtgcc aacagaagac gaagaacagc agcttgaagc cgtgcgccag ttcaaacagg 720

cccagcattt gcgtatcgca gccggggata tttccggggc attgccggtg atgaaagtca 780

gtgaccattt aacctacctt gccgaggcca ttctcgatgt cgtggtgcag catgcgtggg 840

aacaaatggt cgtaaaatac gggcagcccg cgcatcttca gcaccgtgag gggcgcggtt 900

ttgccgtggt cggttacggg aaactcggtg gctgggagct gggttatagc tcagatctgg 960

atctggtctt cctgctcgat tgcgcgccgg aggtgatgac ggacggcgaa cgcagcatcg 1020

acggacgtca gttttatctt cggctggcgc agcgcattat gcacttattc agcaccggga 1080

catcgtcagg cattctttac gaggttgatc cgcgtctgcg accttccggc gcatccggca 1140

tgctggtcag taccattgaa gcgtttgcag attatcaggc caatgaagcc tggacgtggg 1200

agcatcaggc gctggttcgc gcgcgcgtgg tttacgggga tccgcaactg acacagcaat 1260

ttaacgccac gcgtcgcgac attctttgcc gccagcgcga tggcgacggc ctgcgtaagg 1320

1380

ttgatctgaa agccgatccg ggtggcatca cggatattga attcattgca caatacctgg 1440

ttctgcgttt cgcgcatgat gagccgaagc tgacgcgctg gtctgataac gtgcggattt 1500

ttgaactgat ggcacgatat gacatcatgc cggaagagga agcgcgccat ctgacgcagg 1560

cttatgtgac gctgcgcgat gaaattcatc atctggcgtt gcaggaacac agcgggaaag 1620

tggccgcgga cagctttgct actgagcgcg cgcagatccg tgccagctgg gcaaagtggc 1680

tcggctgagg gtttttattc ggctaacagg cgcttgtgat attatccggc gcattgtatt 1740

tacccgattt gatttatctg ttttggagtc ttgggatgaa agtgactttg cctgattttc 1800

accgcgcagg tgtgctggtt gtcggtgacg taatgttaga ccgttactgg tatggcccga 1860

ccaatcgtat ttctccggaa gctccggtgc cggtggtgaa ggtcagtacc attgaagagc 1920

ggcctggcgg tgcagctaac gtggcgatga acatttcatc tctgggcgcc tcttcctgtc 1980

tgatcggcct gaccggcgta gacgacgctg cgcgtgccct cagtgagcgt ctggcagaag 2040

tgaaagttaa ctgcgatttc gtcgcactat ccacacatcc taccatcacc aaactgcgaa 2100

ttttgtcccg taaccagcaa ctgatccgcc tcgactttga ggaaggtttt gaaggcgttg 2160

atctcgagcc gatgctgacc aaaataga 2188

<210> 213

<211> 635

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm6.1

<400> 213

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gatttcgctg 120

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 180

240

agcgttattg aatcgcacat tttaaactgt tggccgctgt ggaagcgaat attggtgaaa 300

ggtgcggttt taaggccttt ttctttgact ctctgtcgtt acaaagttaa tatgcgcgcc 360

ctccgtctct gaagctctcg gtgaacattg ttgcgaggca ggatgcgagc tggttgtgtt 420

ttgacattac cgataatgtg ccgcgtgaac gggtgcgtta tgcccgcccg gaagcggcgt 480

tttcccgtcc ggggaatggc atggagctgc gccttatcca gacgctgatc gcccatcatc 540

gcggttcttt agatctctcg gtccgccctg atggcggcac cttgctgacg ttacgcctgc 600

cggtacagca ggttatcacc ggaggcttaa aatga 635

<210> 214

<211> 1635

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm6.1 with a 500 bp flanking region.

<400> 214

tgtttcgtct cgaggccggg caactgagcg gccccgttga aaccgacctg ggctggcatc 60

tgttgttgtg cgaacaaatt cgcctgccgc aacccttgcc gaaagccgaa gccttaacgc 120

gggtgcgtca gcaactgatt gcccggcaac agaaacatta tcagcgccag tggctgcaac 180

aactgatcaa cgcctgagcc tgttctcctt cttgttgatg cagacgggtt aatgcccgtt 240

ttgcacgaaa aatgcacata aattgcctgc gttgccttat aacagcgcag ggaaatcctg 300

cctccggcct tgtgccacac cgcgctttgc ctggtttgtg gtaaaaactg gcccgctttg 360

catcctgatg cttaaaacac cccgttcaga tcaacctttg ggcagataag cccgcgaaag 420

gcctgcaaat tgcacggtta ttccgggtga gtatatgtgt gatttgggtt ccggcattgc 480

gcaataaagg ggagaaagac atgagcatca cggcgttatc agcatcattt cctgagggga 540

atatcgccag ccgcttgtcg ctgcaacatc cttcactgtt ttataccgtg gttgaacaat 600

cttcggtggc gatttcgctg accgatccgc aggcgcgcat ttgttatgcc aatccggcat 660

tctgccgcca gacgggtttt gcacttgaga cacttttggg cgagaaccac cgtctgctgg 720

aatttttttt cacaaagcgt agcgttattg aatcgcacat tttaaactgt tggccgctgt 780

ggaagcgaat attggtgaaa ggtgcggttt taaggccttt ttctttgact ctctgtcgtt 840

acaaagttaa tatgcgcgcc ctccgtctct gaagctctcg gtgaacattg ttgcgaggca 900

ggatgcgagc tggttgtgtt ttgacattac cgataatgtg ccgcgtgaac gggtgcgtta 960

tgcccgcccg gaagcggcgt tttcccgtcc ggggaatggc atggagctgc gccttatcca 1020

gacgctgatc gcccatcatc gcggttcttt agatctctcg gtccgccctg atggcggcac 1080

cttgctgacg ttacgcctgc cggtacagca ggttatcacc ggaggcttaa aatgacccag 1140

ttacctaccg cgggcccggt tatccggcgc tttgatatgt ctgcccagtt tacggcgctt 1200

tatcgcatca gcgtggcgct gagtcaggaa agcaacaccg ggcgcgcact ggcggcgatc 1260

1320

gaacgcaatg cactctttgt ggaatccctg catggcatcg acggcgaaag gaaaaaagag 1380

acccgccatg tccgttaccg catgggggaa ggcgtgatcg gcgcggtgat gagccagcgt 1440

caggcgctgg tgttaccgcg catttcagac gatcagcgtt ttctcgaccg cctgaatatt 1500

tacgattaca gcctgccgtt gattggcgtg ccgatccccg gtgcggataa tcagccatcg 1560

ggcgtgctgg tggcacagcc gatggcgttg cacgaagacc ggctgactgc cagtacgcgg 1620

tttttagaaa tggtc 1635

<210> 215

<211> 635

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm6.1

<400> 215

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gatttcgctg 120

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 180

240

agcgttattg aatcgcacat tttaaactgt tggccgctgt ggaagcgaat attggtgaaa 300

ggtgcggttt taaggccttt ttctttgact ctctgtcgtt acaaagttaa tatgcgcgcc 360

ctccgtctct gaagctctcg gtgaacattg ttgcgaggca ggatgcgagc tggttgtgtt 420

ttgacattac cgataatgtg ccgcgtgaac gggtgcgtta tgcccgcccg gaagcggcgt 480

tttcccgtcc ggggaatggc atggagctgc gccttatcca gacgctgatc gcccatcatc 540

gcggttcttt agatctctcg gtccgccctg atggcggcac cttgctgacg ttacgcctgc 600

cggtacagca ggttatcacc ggaggcttaa aatga 635

<210> 216

<211> 1635

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm6.1 with a 500 bp flanking region.

<400> 216

tgtttcgtct cgaggccggg caactgagcg gccccgttga aaccgacctg ggctggcatc 60

tgttgttgtg cgaacaaatt cgcctgccgc aacccttgcc gaaagccgaa gccttaacgc 120

gggtgcgtca gcaactgatt gcccggcaac agaaacatta tcagcgccag tggctgcaac 180

aactgatcaa cgcctgagcc tgttctcctt cttgttgatg cagacgggtt aatgcccgtt 240

ttgcacgaaa aatgcacata aattgcctgc gttgccttat aacagcgcag ggaaatcctg 300

cctccggcct tgtgccacac cgcgctttgc ctggtttgtg gtaaaaactg gcccgctttg 360

catcctgatg cttaaaacac cccgttcaga tcaacctttg ggcagataag cccgcgaaag 420

gcctgcaaat tgcacggtta ttccgggtga gtatatgtgt gatttgggtt ccggcattgc 480

gcaataaagg ggagaaagac atgagcatca cggcgttatc agcatcattt cctgagggga 540

atatcgccag ccgcttgtcg ctgcaacatc cttcactgtt ttataccgtg gttgaacaat 600

cttcggtggc gatttcgctg accgatccgc aggcgcgcat ttgttatgcc aatccggcat 660

tctgccgcca gacgggtttt gcacttgaga cacttttggg cgagaaccac cgtctgctgg 720

aatttttttt cacaaagcgt agcgttattg aatcgcacat tttaaactgt tggccgctgt 780

ggaagcgaat attggtgaaa ggtgcggttt taaggccttt ttctttgact ctctgtcgtt 840

acaaagttaa tatgcgcgcc ctccgtctct gaagctctcg gtgaacattg ttgcgaggca 900

ggatgcgagc tggttgtgtt ttgacattac cgataatgtg ccgcgtgaac gggtgcgtta 960

tgcccgcccg gaagcggcgt tttcccgtcc ggggaatggc atggagctgc gccttatcca 1020

gacgctgatc gcccatcatc gcggttcttt agatctctcg gtccgccctg atggcggcac 1080

cttgctgacg ttacgcctgc cggtacagca ggttatcacc ggaggcttaa aatgacccag 1140

ttacctaccg cgggcccggt tatccggcgc tttgatatgt ctgcccagtt tacggcgctt 1200

tatcgcatca gcgtggcgct gagtcaggaa agcaacaccg ggcgcgcact ggcggcgatc 1260

1320

gaacgcaatg cactctttgt ggaatccctg catggcatcg acggcgaaag gaaaaaagag 1380

acccgccatg tccgttaccg catgggggaa ggcgtgatcg gcgcggtgat gagccagcgt 1440

caggcgctgg tgttaccgcg catttcagac gatcagcgtt ttctcgaccg cctgaatatt 1500

tacgattaca gcctgccgtt gattggcgtg ccgatccccg gtgcggataa tcagccatcg 1560

ggcgtgctgg tggcacagcc gatggcgttg cacgaagacc ggctgactgc cagtacgcgg 1620

tttttagaaa tggtc 1635

<210> 217

<211> 786

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm7.1

<400> 217

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gatttcgctg 120

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 180

gcacttgaga cacttttggg cgagaaccac cgtctgctgg ttaaaaacgt gaccacgagc 240

attaataaac gccacgaaat gtggcgttta tttattcaaa aagtatcttc tttcataaaa 300

agtgctaaat gcagtagcag caaaattgggg ataagtccca tggaatacgg ctgttttcgc 360

tgcaattttt aactttttcg taaaaaaaga tgtttctttg agcgaacgat caaaatatag 420

cgttaaccgg caaaaaatta ttctcattag aaaatagttt gtgtaatact tgtaacgcta 480

catggagatt aacttaatct aggggtttt ataccgtctc tgaagctctc ggtgaacatt 540

gttgcgaggc aggatgcgag ctggttgtgt tttgacatta ccgataatgt gccgcgtgaa 600

cgggtgcgtt atgcccgcc ggaagcggcg ttttcccgtc cggggaatgg catggagctg 660

cgccttatcc agacgctgat cgcccatcat cgcggttctt tagatctctc ggtccgccct 720

gatggcggca ccttgctgac gttacgcctg ccggtacagc aggttatcac cggaggctta 780

aaatga 786

<210> 218

<211> 1786

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm7.1 with a 500 bp flanking region.

<400> 218

gtttcgtctc gaggccgggc aactgagcgg ccccgttgaa accgacctgg gctggcatct 60

gttgttgtgc gaacaaattc gcctgccgca acccttgccg aaagccgaag ccttaacgcg 120

ggtgcgtcag caactgattg cccggcaaca gaaacattat cagcgccagt ggctgcaaca 180

actgatcaac gcctgagcct gttctccttc ttgttgatgc agacgggtta atgcccgttt 240

tgcacgaaaa atgcacataa attgcctgcg ttgccttata acagcgcagg gaaatcctgc 300

ctccggcctt gtgccacacc gcgctttgcc tggtttgtgg taaaaactgg cccgctttgc 360

atcctgatgc ttaaaacacc ccgttcagat caacctttgg gcagataagc ccgcgaaagg 420

cctgcaaatt gcacggttat tccgggtgag tatatgtgtg atttgggttc cggcattgcg 480

caataaaggg gagaaagaca tgagcatcac ggcgttatca gcatcatttc ctgaggggaa 540

tatcgccagc cgcttgtcgc tgcaacatcc ttcactgttt tataccgtgg ttgaacaatc 600

ttcggtggcg atttcgctga ccgatccgca ggcgcgcatt tgttatgcca atccggcatt 660

ctgccgccag acgggttttg cacttgagac acttttgggc gagaaccacc gtctgctggt 720

780

agtatcttct ttcataaaaa gtgctaaatg cagtagcagc aaaattggga taagtcccat 840

ggaatacggc tgttttcgct gcaattttta actttttcgt aaaaaaagat gtttctttga 900

gcgaacgatc aaaatatagc gttaaccggc aaaaaattat tctcattaga aaatagtttg 960

tgtaatactt gtaacgctac atggagatta acttaatcta gagggtttta taccgtctct 1020

gaagctctcg gtgaacattg ttgcgaggca ggatgcgagc tggttgtgtt ttgacattac 1080

1140

ggggaatggc atggagctgc gccttatcca gacgctgatc gcccatcatc gcggttcttt 1200

agatctctcg gtccgccctg atggcggcac cttgctgacg ttacgcctgc cggtacagca 1260

ggttatcacc ggaggcttaa aatgacccag ttacctaccg cgggcccggt tatccggcgc 1320

tttgatatgt ctgcccagtt tacggcgctt tatcgcatca gcgtggcgct gagtcaggaa 1380

agcaacaccg ggcgcgcact ggcggcgatc ctcgaagtgc ttcacgatca tgcatttatg 1440

caatacggca tggtgtgtct gtttgataaa gaacgcaatg cactctttgt ggaatccctg 1500

catggcatcg acggcgaaag gaaaaaagag acccgccatg tccgttaccg catgggggaa 1560

ggcgtgatcg gcgcggtgat gagccagcgt caggcgctgg tgttaccgcg catttcagac 1620

gatcagcgtt ttctcgaccg cctgaatatt tacgattaca gcctgccgtt gattggcgtg 1680

ccgatccccg gtgcggataa tcagccatcg ggcgtgctgg tggcacagcc gatggcgttg 1740

cacgaagacc ggctgactgc cagtacgcgg tttttagaaa tggtcg 1786

<210> 219

<211> 993

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1.2

<400> 219

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gatttcgctg 120

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 180

240

gctacctatg tcgaagaatt aactaaaaaa ctgcaagatg caggcattcg cgttaaagcc 300

360

tatatgttag tttgtggcga taaagaggtc gaagcaggca aagttgctgt tcgtacccgc 420

cgcggcaaag acttaggaag catggatgtt agcgaagtcg ttgacaaact gctggcggaa 480

atccgcagca gaagtcttca tcaactggag gaataaagta ttaaaggcgg aaaacgagtt 540

caaccggcgc gtcctaatcg cattaacaaa gagattcgcg cgcaagaagt tcgcctcaca 600

ggcgtcgatg gcgagcagat tggtattgtc agtctgaatg aagctcttga aaaagctgag 660

gaagcgggcg tcgatttagt agaaatcagt ccgaatgccg agccgccagt ttgtcgaatc 720

ccgtctctga agctctcggt gaacattgtt gcgaggcagg atgcgagctg gttgtgtttt 780

gacattaccg ataatgtgcc gcgtgaacgg gtgcgttatg cccgcccggga agcggcgttt 840

tcccgtccgg ggaatggcat ggagctgcgc cttatccaga cgctgatcgc ccatcatcgc 900

ggttctttag atctctcggt ccgccctgat ggcggcacct tgctgacgtt acgcctgccg 960

gtacagcagg ttatcaccgg aggcttaaaa tga 993

<210> 220

<211> 1993

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1.2 with a 500 bp flanking region.

<400> 220

tgtttcgtct cgaggccggg caactgagcg gccccgttga aaccgacctg ggctggcatc 60

tgttgttgtg cgaacaaatt cgcctgccgc aacccttgcc gaaagccgaa gccttaacgc 120

gggtgcgtca gcaactgatt gcccggcaac agaaacatta tcagcgccag tggctgcaac 180

aactgatcaa cgcctgagcc tgttctcctt cttgttgatg cagacgggtt aatgcccgtt 240

ttgcacgaaa aatgcacata aattgcctgc gttgccttat aacagcgcag ggaaatcctg 300

cctccggcct tgtgccacac cgcgctttgc ctggtttgtg gtaaaaactg gcccgctttg 360

catcctgatg cttaaaacac cccgttcaga tcaacctttg ggcagataag cccgcgaaag 420

gcctgcaaat tgcacggtta ttccgggtga gtatatgtgt gatttgggtt ccggcattgc 480

gcaataaagg ggagaaagac atgagcatca cggcgttatc agcatcattt cctgagggga 540

atatcgccag ccgcttgtcg ctgcaacatc cttcactgtt ttataccgtg gttgaacaat 600

cttcggtggc gatttcgctg accgatccgc aggcgcgcat ttgttatgcc aatccggcat 660

tctgccgcca gacgggtttt gcacttgaga cacttttggg cgagaaccac cgtctgctgg 720

tgaacatcac tgatgcacaa gctacctatg tcgaagaatt aactaaaaaa ctgcaagatg 780

caggcattcg cgttaaagcc gacttgagaa atgagaagat tggctttaaa attcgcgaac 840

acacgctacg ccgtgttcct tatatgttag tttgtggcga taaagaggtc gaagcaggca 900

aagttgctgt tcgtacccgc cgcggcaaag acttaggaag catggatgtt agcgaagtcg 960

ttgacaaact gctggcggaa atccgcagca gaagtcttca tcaactggag gaataaagta 1020

ttaaaggcgg aaaacgagtt caaccggcgc gtcctaatcg cattaacaaa gagattcgcg 1080

cgcaagaagt tcgcctcaca ggcgtcgatg gcgagcagat tggtattgtc agtctgaatg 1140

aagctcttga aaaagctgag gaagcgggcg tcgatttagt agaaatcagt ccgaatgccg 1200

agccgccagt ttgtcgaatc ccgtctctga agctctcggt gaacattgtt gcgaggcagg 1260

atgcgagctg gttgtgtttt gacattaccg ataatgtgcc gcgtgaacgg gtgcgttatg 1320

cccgcccggga agcggcgttt tcccgtccgg ggaatggcat ggagctgcgc cttatccaga 1380

cgctgatcgc ccatcatcgc ggttctttag atctctcggt ccgccctgat ggcggcacct 1440

tgctgacgtt acgcctgccg gtacagcagg ttatcaccgg aggcttaaaa tgacccagtt 1500

acctaccgcg ggcccggtta tccggcgctt tgatatgtct gcccagttta cggcgcttta 1560

tcgcatcagc gtggcgctga gtcaggaaag caacaccggg cgcgcactgg cggcgatcct 1620

cgaagtgctt cacgatcatg catttatgca atacggcatg gtgtgtctgt ttgataaaga 1680

acgcaatgca ctctttgtgg aatccctgca tggcatcgac ggcgaaagga aaaaagagac 1740

ccgccatgtc cgttaccgca tgggggaagg cgtgatcggc gcggtgatga gccagcgtca 1800

1860

cgattacagc ctgccgttga ttggcgtgcc gatccccggt gcggataatc agccatcggg 1920

cgtgctggtg gcacagccga tggcgttgca cgaagaccgg ctgactgcca gtacgcggtt 1980

tttagaaatg gtc 1993

<210> 221

<211> 993

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1.2

<400> 221

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gatttcgctg 120

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 180

240

gctacctatg tcgaagaatt aactaaaaaa ctgcaagatg caggcattcg cgttaaagcc 300

360

tatatgttag tttgtggcga taaagaggtc gaagcaggca aagttgctgt tcgtacccgc 420

cgcggcaaag acttaggaag catggatgtt agcgaagtcg ttgacaaact gctggcggaa 480

atccgcagca gaagtcttca tcaactggag gaataaagta ttaaaggcgg aaaacgagtt 540

caaccggcgc gtcctaatcg cattaacaaa gagattcgcg cgcaagaagt tcgcctcaca 600

ggcgtcgatg gcgagcagat tggtattgtc agtctgaatg aagctcttga aaaagctgag 660

gaagcgggcg tcgatttagt agaaatcagt ccgaatgccg agccgccagt ttgtcgaatc 720

ccgtctctga agctctcggt gaacattgtt gcgaggcagg atgcgagctg gttgtgtttt 780

gacattaccg ataatgtgcc gcgtgaacgg gtgcgttatg cccgcccggga agcggcgttt 840

tcccgtccgg ggaatggcat ggagctgcgc cttatccaga cgctgatcgc ccatcatcgc 900

ggttctttag atctctcggt ccgccctgat ggcggcacct tgctgacgtt acgcctgccg 960

gtacagcagg ttatcaccgg aggcttaaaa tga 993

<210> 222

<211> 1993

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1.2 with a 500 bp flanking region.

<400> 222

tgtttcgtct cgaggccggg caactgagcg gccccgttga aaccgacctg ggctggcatc 60

tgttgttgtg cgaacaaatt cgcctgccgc aacccttgcc gaaagccgaa gccttaacgc 120

gggtgcgtca gcaactgatt gcccggcaac agaaacatta tcagcgccag tggctgcaac 180

aactgatcaa cgcctgagcc tgttctcctt cttgttgatg cagacgggtt aatgcccgtt 240

ttgcacgaaa aatgcacata aattgcctgc gttgccttat aacagcgcag ggaaatcctg 300

cctccggcct tgtgccacac cgcgctttgc ctggtttgtg gtaaaaactg gcccgctttg 360

catcctgatg cttaaaacac cccgttcaga tcaacctttg ggcagataag cccgcgaaag 420

gcctgcaaat tgcacggtta ttccgggtga gtatatgtgt gatttgggtt ccggcattgc 480

gcaataaagg ggagaaagac atgagcatca cggcgttatc agcatcattt cctgagggga 540

atatcgccag ccgcttgtcg ctgcaacatc cttcactgtt ttataccgtg gttgaacaat 600

cttcggtggc gatttcgctg accgatccgc aggcgcgcat ttgttatgcc aatccggcat 660

tctgccgcca gacgggtttt gcacttgaga cacttttggg cgagaaccac cgtctgctgg 720

tgaacatcac tgatgcacaa gctacctatg tcgaagaatt aactaaaaaa ctgcaagatg 780

caggcattcg cgttaaagcc gacttgagaa atgagaagat tggctttaaa attcgcgaac 840

acacgctacg ccgtgttcct tatatgttag tttgtggcga taaagaggtc gaagcaggca 900

aagttgctgt tcgtacccgc cgcggcaaag acttaggaag catggatgtt agcgaagtcg 960

ttgacaaact gctggcggaa atccgcagca gaagtcttca tcaactggag gaataaagta 1020

ttaaaggcgg aaaacgagtt caaccggcgc gtcctaatcg cattaacaaa gagattcgcg 1080

cgcaagaagt tcgcctcaca ggcgtcgatg gcgagcagat tggtattgtc agtctgaatg 1140

aagctcttga aaaagctgag gaagcgggcg tcgatttagt agaaatcagt ccgaatgccg 1200

agccgccagt ttgtcgaatc ccgtctctga agctctcggt gaacattgtt gcgaggcagg 1260

atgcgagctg gttgtgtttt gacattaccg ataatgtgcc gcgtgaacgg gtgcgttatg 1320

cccgcccggga agcggcgttt tcccgtccgg ggaatggcat ggagctgcgc cttatccaga 1380

cgctgatcgc ccatcatcgc ggttctttag atctctcggt ccgccctgat ggcggcacct 1440

tgctgacgtt acgcctgccg gtacagcagg ttatcaccgg aggcttaaaa tgacccagtt 1500

acctaccgcg ggcccggtta tccggcgctt tgatatgtct gcccagttta cggcgcttta 1560

tcgcatcagc gtggcgctga gtcaggaaag caacaccggg cgcgcactgg cggcgatcct 1620

cgaagtgctt cacgatcatg catttatgca atacggcatg gtgtgtctgt ttgataaaga 1680

acgcaatgca ctctttgtgg aatccctgca tggcatcgac ggcgaaagga aaaaagagac 1740

ccgccatgtc cgttaccgca tgggggaagg cgtgatcggc gcggtgatga gccagcgtca 1800

1860

cgattacagc ctgccgttga ttggcgtgcc gatccccggt gcggataatc agccatcggg 1920

cgtgctggtg gcacagccga tggcgttgca cgaagaccgg ctgactgcca gtacgcggtt 1980

tttagaaatg gtc 1993

<210> 223

<211> 1188

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2

<400> 223

atggcgctca aacagttaat ccgtctgtgt gccgcctcgc cgatggtcgc gacacaactt 60

gcacgtcatc ctttattgct cgatgaactg ctcgacccgc gcacgcttta ccagccgatt 120

gagccgggcg cttaccgcga cgaactgcgt cagtatctga tgcgggtgcc aacagaagac 180

gaagaacagc agcttgaagc cgtgcgccag ttcaaacagg cccagcattt gcgtatcgca 240

gccggggata tttccggggc attgccggtg atgaaagtca gtgaccattt aacctacctt 300

gccgaggcca ttctcgatgt cgtggtgcag catgcgtggg aacaaatggt cgtaaaatac 360

gggcagcccg cgcatcttca gcaccgtgag gggcgcggtt ttgccgtggt cggttacggg 420

aaactcggtg gctgggagct gggttatagc tcagatctgg atctggtctt cctgctcgat 480

tgcgcgccgg aggtgatgac ggacggcgaa cgcagcatcg acggacgtca gttttatctt 540

cggctggcgc agcgcattat gcacttattc agcaccggga catcgtcagg cattctttac 600

gaggttgatc cgcgtctgcg accttccggc gcatccggca tgctggtcag taccattgaa 660

gcgtttgcag attatcaggc caatgaagcc tggacgtggg agcatcaggc gctggttcgc 720

gcgcgcgtgg tttacgggga tccgcaactg acacagcaat ttaacgccac gcgtcgcgac 780

attctttgcc gccagcgcga tggcgacggc ctgcgtaagg aggtccgtga aatgcgcgag 840

aaaatgtatg cccatctggg gagtaaaaaa gcccacgagt ttgatctgaa agccgatccg 900

ggtggcatca cggatattga attcattgca caatacctgg ttctgcgttt cgcgcatgat 960

gagccgaagc tgacgcgctg gtctgataac gtgcggattt ttgaactgat ggcacgatat 1020

gacatcatgc cggaagagga agcgcgccat ctgacgcagg cttatgtgac gctgcgcgat 1080

gaaattcatc atctggcgtt gcaggaacac agcgggaaag tggccgcgga cagctttgct 1140

actgagcgcg cgcagatccg tgccagctgg gcaaagtggc tcggctga 1188

<210> 224

<211> 2188

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2 with a 500 bp flanking region.

<400> 224

cggtactgga acagaaatcg gcggatgcgc aggaaatttg ttatgacacg gcctgtctga 60

agtgcaagtt agtgcttact tcctggctgg caacctcagg ctggacgccg tttattgatg 120

ataaatctgc gaagaaactg gacgcttcct tcaaacgttt tgctgacatc atgctcggtc 180

gtaccgcagc ggatctgaaa gaagcctttg cgcagccact gacggaagaa ggttatcgcg 240

atcagctggc gcgcctgaaa cgccagatca ttaccttcca tttgcttgcc ggtgcttacc 300

ctgaaaaaga cgtcgatgcg tatattgccg gctgggtgga cctgcaacag gccatcgttc 360

agcagcaaca cgcctgggag gattcggccc gttctcacgc ggtgatgatg gatgctttct 420

ggttaaacgg gcaacctcgt taactgactg actagcctgg gcaaactgcc cgggcttttt 480

tttgcaagga atctgatttc atggcgctca aacagttaat ccgtctgtgt gccgcctcgc 540

cgatggtcgc gacacaactt gcacgtcatc ctttattgct cgatgaactg ctcgacccgc 600

gcacgcttta ccagccgatt gagccgggcg cttaccgcga cgaactgcgt cagtatctga 660

tgcgggtgcc aacagaagac gaagaacagc agcttgaagc cgtgcgccag ttcaaacagg 720

cccagcattt gcgtatcgca gccggggata tttccggggc attgccggtg atgaaagtca 780

gtgaccattt aacctacctt gccgaggcca ttctcgatgt cgtggtgcag catgcgtggg 840

aacaaatggt cgtaaaatac gggcagcccg cgcatcttca gcaccgtgag gggcgcggtt 900

ttgccgtggt cggttacggg aaactcggtg gctgggagct gggttatagc tcagatctgg 960

atctggtctt cctgctcgat tgcgcgccgg aggtgatgac ggacggcgaa cgcagcatcg 1020

acggacgtca gttttatctt cggctggcgc agcgcattat gcacttattc agcaccggga 1080

catcgtcagg cattctttac gaggttgatc cgcgtctgcg accttccggc gcatccggca 1140

tgctggtcag taccattgaa gcgtttgcag attatcaggc caatgaagcc tggacgtggg 1200

agcatcaggc gctggttcgc gcgcgcgtgg tttacgggga tccgcaactg acacagcaat 1260

ttaacgccac gcgtcgcgac attctttgcc gccagcgcga tggcgacggc ctgcgtaagg 1320

1380

ttgatctgaa agccgatccg ggtggcatca cggatattga attcattgca caatacctgg 1440

ttctgcgttt cgcgcatgat gagccgaagc tgacgcgctg gtctgataac gtgcggattt 1500

ttgaactgat ggcacgatat gacatcatgc cggaagagga agcgcgccat ctgacgcagg 1560

cttatgtgac gctgcgcgat gaaattcatc atctggcgtt gcaggaacac agcgggaaag 1620

tggccgcgga cagctttgct actgagcgcg cgcagatccg tgccagctgg gcaaagtggc 1680

tcggctgagg gtttttattc ggctaacagg cgcttgtgat attatccggc gcattgtatt 1740

tacccgattt gatttatctg ttttggagtc ttgggatgaa agtgactttg cctgattttc 1800

accgcgcagg tgtgctggtt gtcggtgacg taatgttaga ccgttactgg tatggcccga 1860

ccaatcgtat ttctccggaa gctccggtgc cggtggtgaa ggtcagtacc attgaagagc 1920

ggcctggcgg tgcagctaac gtggcgatga acatttcatc tctgggcgcc tcttcctgtc 1980

tgatcggcct gaccggcgta gacgacgctg cgcgtgccct cagtgagcgt ctggcagaag 2040

tgaaagttaa ctgcgatttc gtcgcactat ccacacatcc taccatcacc aaactgcgaa 2100

ttttgtcccg taaccagcaa ctgatccgcc tcgactttga ggaaggtttt gaaggcgttg 2160

atctcgagcc gatgctgacc aaaataga 2188

<210> 225

<211> 663

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm3.1

<400> 225

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gatttcgctg 120

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 180

gcacttgaga cacttttggg cgagaaccac cgtctgctgg tacagtagcg cctctcaaaa 240

atagataaac ggctcatgta cgtgggccgt ttatttttttc tacccataat cgggaaccgg 300

tgttataatg ccgcgccctc atattgtggg gatttcttaa tgacctatcc tgggtcctaa 360

agttgtagtt gacattagcg gagcactaac ccgtctctga agctctcggt gaacattgtt 420

gcgaggcagg atgcgagctg gttgtgtttt gacattaccg ataatgtgcc gcgtgaacgg 480

gtgcgttatg cccgcccgga agcggcgttt tcccgtccgg ggaatggcat ggagctgcgc 540

cttatccaga cgctgatcgc ccatcatcgc ggttctttag atctctcggt ccgccctgat 600

ggcggcacct tgctgacgtt acgcctgccg gtacagcagg ttatcaccgg aggcttaaaa 660

tga 663

<210> 226

<211> 1663

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm3.1 with a 500 bp flanking region.

<400> 226

tgtttcgtct cgaggccggg caactgagcg gccccgttga aaccgacctg ggctggcatc 60

tgttgttgtg cgaacaaatt cgcctgccgc aacccttgcc gaaagccgaa gccttaacgc 120

gggtgcgtca gcaactgatt gcccggcaac agaaacatta tcagcgccag tggctgcaac 180

aactgatcaa cgcctgagcc tgttctcctt cttgttgatg cagacgggtt aatgcccgtt 240

ttgcacgaaa aatgcacata aattgcctgc gttgccttat aacagcgcag ggaaatcctg 300

cctccggcct tgtgccacac cgcgctttgc ctggtttgtg gtaaaaactg gcccgctttg 360

catcctgatg cttaaaacac cccgttcaga tcaacctttg ggcagataag cccgcgaaag 420

gcctgcaaat tgcacggtta ttccgggtga gtatatgtgt gatttgggtt ccggcattgc 480

gcaataaagg ggagaaagac atgagcatca cggcgttatc agcatcattt cctgagggga 540

atatcgccag ccgcttgtcg ctgcaacatc cttcactgtt ttataccgtg gttgaacaat 600

cttcggtggc gatttcgctg accgatccgc aggcgcgcat ttgttatgcc aatccggcat 660

tctgccgcca gacgggtttt gcacttgaga cacttttggg cgagaaccac cgtctgctgg 720

tacagtagcg cctctcaaaa atagataaac ggctcatgta cgtgggccgt ttatttttttc 780

tacccataat cgggaaccgg tgttataatg ccgcgccctc atattgtggg gatttcttaa 840

tgacctatcc tgggtcctaa agttgtagtt gacattagcg gagcactaac ccgtctctga 900

agctctcggt gaacattgtt gcgaggcagg atgcgagctg gttgtgtttt gacattaccg 960

ataatgtgcc gcgtgaacgg gtgcgttatg cccgcccggga agcggcgttt tcccgtccgg 1020

ggaatggcat ggagctgcgc cttatccaga cgctgatcgc ccatcatcgc ggttctttag 1080

atctctcggt ccgccctgat ggcggcacct tgctgacgtt acgcctgccg gtacagcagg 1140

ttatcaccgg aggcttaaaa tgacccagtt acctaccgcg ggcccggtta tccggcgctt 1200

tgatatgtct gcccagttta cggcgcttta tcgcatcagc gtggcgctga gtcaggaaag 1260

caacaccggg cgcgcactgg cggcgatcct cgaagtgctt cacgatcatg catttatgca 1320

atacggcatg gtgtgtctgt ttgataaaga acgcaatgca ctctttgtgg aatccctgca 1380

tggcatcgac ggcgaaagga aaaaagagac ccgccatgtc cgttaccgca tgggggaagg 1440

cgtgatcggc gcggtgatga gccagcgtca ggcgctggtg ttaccgcgca tttcagacga 1500

tcagcgtttt ctcgaccgcc tgaatattta cgattacagc ctgccgttga ttggcgtgcc 1560

gatccccggt gcggataatc agccatcggg cgtgctggtg gcacagccga tggcgttgca 1620

cgaagaccgg ctgactgcca gtacgcggtt tttagaaatg gtc 1663

<210> 227

<211> 1188

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2

<400> 227

atggcgctca aacagttaat ccgtctgtgt gccgcctcgc cgatggtcgc gacacaactt 60

gcacgtcatc ctttattgct cgatgaactg ctcgacccgc gcacgcttta ccagccgatt 120

gagccgggcg cttaccgcga cgaactgcgt cagtatctga tgcgggtgcc aacagaagac 180

gaagaacagc agcttgaagc cgtgcgccag ttcaaacagg cccagcattt gcgtatcgca 240

gccggggata tttccggggc attgccggtg atgaaagtca gtgaccattt aacctacctt 300

gccgaggcca ttctcgatgt cgtggtgcag catgcgtggg aacaaatggt cgtaaaatac 360

gggcagcccg cgcatcttca gcaccgtgag gggcgcggtt ttgccgtggt cggttacggg 420

aaactcggtg gctgggagct gggttatagc tcagatctgg atctggtctt cctgctcgat 480

tgcgcgccgg aggtgatgac ggacggcgaa cgcagcatcg acggacgtca gttttatctt 540

cggctggcgc agcgcattat gcacttattc agcaccggga catcgtcagg cattctttac 600

gaggttgatc cgcgtctgcg accttccggc gcatccggca tgctggtcag taccattgaa 660

gcgtttgcag attatcaggc caatgaagcc tggacgtggg agcatcaggc gctggttcgc 720

gcgcgcgtgg tttacgggga tccgcaactg acacagcaat ttaacgccac gcgtcgcgac 780

attctttgcc gccagcgcga tggcgacggc ctgcgtaagg aggtccgtga aatgcgcgag 840

aaaatgtatg cccatctggg gagtaaaaaa gcccacgagt ttgatctgaa agccgatccg 900

ggtggcatca cggatattga attcattgca caatacctgg ttctgcgttt cgcgcatgat 960

gagccgaagc tgacgcgctg gtctgataac gtgcggattt ttgaactgat ggcacgatat 1020

gacatcatgc cggaagagga agcgcgccat ctgacgcagg cttatgtgac gctgcgcgat 1080

gaaattcatc atctggcgtt gcaggaacac agcgggaaag tggccgcgga cagctttgct 1140

actgagcgcg cgcagatccg tgccagctgg gcaaagtggc tcggctga 1188

<210> 228

<211> 2188

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2 with a 500 bp flanking region.

<400> 228

cggtactgga acagaaatcg gcggatgcgc aggaaatttg ttatgacacg gcctgtctga 60

agtgcaagtt agtgcttact tcctggctgg caacctcagg ctggacgccg tttattgatg 120

ataaatctgc gaagaaactg gacgcttcct tcaaacgttt tgctgacatc atgctcggtc 180

gtaccgcagc ggatctgaaa gaagcctttg cgcagccact gacggaagaa ggttatcgcg 240

atcagctggc gcgcctgaaa cgccagatca ttaccttcca tttgcttgcc ggtgcttacc 300

ctgaaaaaga cgtcgatgcg tatattgccg gctgggtgga cctgcaacag gccatcgttc 360

agcagcaaca cgcctgggag gattcggccc gttctcacgc ggtgatgatg gatgctttct 420

ggttaaacgg gcaacctcgt taactgactg actagcctgg gcaaactgcc cgggcttttt 480

tttgcaagga atctgatttc atggcgctca aacagttaat ccgtctgtgt gccgcctcgc 540

cgatggtcgc gacacaactt gcacgtcatc ctttattgct cgatgaactg ctcgacccgc 600

gcacgcttta ccagccgatt gagccgggcg cttaccgcga cgaactgcgt cagtatctga 660

tgcgggtgcc aacagaagac gaagaacagc agcttgaagc cgtgcgccag ttcaaacagg 720

cccagcattt gcgtatcgca gccggggata tttccggggc attgccggtg atgaaagtca 780

gtgaccattt aacctacctt gccgaggcca ttctcgatgt cgtggtgcag catgcgtggg 840

aacaaatggt cgtaaaatac gggcagcccg cgcatcttca gcaccgtgag gggcgcggtt 900

ttgccgtggt cggttacggg aaactcggtg gctgggagct gggttatagc tcagatctgg 960

atctggtctt cctgctcgat tgcgcgccgg aggtgatgac ggacggcgaa cgcagcatcg 1020

acggacgtca gttttatctt cggctggcgc agcgcattat gcacttattc agcaccggga 1080

catcgtcagg cattctttac gaggttgatc cgcgtctgcg accttccggc gcatccggca 1140

tgctggtcag taccattgaa gcgtttgcag attatcaggc caatgaagcc tggacgtggg 1200

agcatcaggc gctggttcgc gcgcgcgtgg tttacgggga tccgcaactg acacagcaat 1260

ttaacgccac gcgtcgcgac attctttgcc gccagcgcga tggcgacggc ctgcgtaagg 1320

1380

ttgatctgaa agccgatccg ggtggcatca cggatattga attcattgca caatacctgg 1440

ttctgcgttt cgcgcatgat gagccgaagc tgacgcgctg gtctgataac gtgcggattt 1500

ttgaactgat ggcacgatat gacatcatgc cggaagagga agcgcgccat ctgacgcagg 1560

cttatgtgac gctgcgcgat gaaattcatc atctggcgtt gcaggaacac agcgggaaag 1620

tggccgcgga cagctttgct actgagcgcg cgcagatccg tgccagctgg gcaaagtggc 1680

tcggctgagg gtttttattc ggctaacagg cgcttgtgat attatccggc gcattgtatt 1740

tacccgattt gatttatctg ttttggagtc ttgggatgaa agtgactttg cctgattttc 1800

accgcgcagg tgtgctggtt gtcggtgacg taatgttaga ccgttactgg tatggcccga 1860

ccaatcgtat ttctccggaa gctccggtgc cggtggtgaa ggtcagtacc attgaagagc 1920

ggcctggcgg tgcagctaac gtggcgatga acatttcatc tctgggcgcc tcttcctgtc 1980

tgatcggcct gaccggcgta gacgacgctg cgcgtgccct cagtgagcgt ctggcagaag 2040

tgaaagttaa ctgcgatttc gtcgcactat ccacacatcc taccatcacc aaactgcgaa 2100

ttttgtcccg taaccagcaa ctgatccgcc tcgactttga ggaaggtttt gaaggcgttg 2160

atctcgagcc gatgctgacc aaaataga 2188

<210> 229

<211> 613

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::PinfC

<400> 229

atgagcatca cggcgttatc agctgaatat cactgactca caagctacct atgtcgaaga 60

attaactaaa aaactgcaag atgcaggcat tcgcgttaaa gccgacttga gaaatgagaa 120

180

cgataaagag gtcgaagcag gcaaagttgc tgttcgtact cgtcgcggca aagacttagg 240

aagcatggat gttagcgaag tcgttgacaa actgctggcg gaaatccgca gcagaagtca 300

tcatcaactg gaggaataaa gtattaaagg cggaaaacga gttcaaccgg cgcgtcctaa 360

tcgcattaac aaagagattc gcgcgcaaga agttcgcctc accggcgtcg atggcgagca 420

gattggtatt gtcagtctga atgaagctct tgaaaaagct gaggaagcgg gcgtcgattt 480

agtagaaatc agtccgaatg ccgagccgcc agtttgtcga atctctttag atctctcggt 540

ccgccctgat ggcggcacct tgctgacgtt acgcctgccg gtacagcagg ttatcaccgg 600

aggcttaaaa tga 613

<210> 230

<211> 1613

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::PinfC with a 500 bp flanking region.

<400> 230

tgtttcgtct cgaagccggg caactgagca gccccgttga aaccgaactg ggctggcatc 60

tgttgttgtg cgaacaaatt cgcctgccgc aacccttgcc gaaagccgag gccttaacgc 120

gggtgcgtca gcaactgatt gcccggcaac agaatcatta tcagcgccag tggctgcaac 180

aactgatcaa cgcctgagcc tgttctcctt cttgttggtg cagacgggtt aatgcccgtt 240

ttgcacgaaa aatgcacata aactgccttc gctgccttat aacagcgcat ggaaatcctg 300

360

catcctgatg tttaaaacac cccgttcaga tcaacctttg ggcagataag cccgcgaaag 420

gcctgcaaat tgcacggtta ttccgggtga gtatatgtgt gatttgggtt ccggcattgc 480

gcaataaagg ggagaaagac atgagcatca cggcgttatc agctgaatat cactgactca 540

caagctacct atgtcgaaga attaactaaa aaactgcaag atgcaggcat tcgcgttaaa 600

gccgacttga gaaatgagaa gattggcttt aaaattcgcg aacacacgct acgccgtgtt 660

ccttatatgt tagtttgtgg cgataaagag gtcgaagcag gcaaagttgc tgttcgtact 720

cgtcgcggca aagacttagg aagcatggat gttagcgaag tcgttgacaa actgctggcg 780

gaaatccgca gcagaagtca tcatcaactg gaggaataaa gtattaaagg cggaaaacga 840

gttcaaccgg cgcgtcctaa tcgcattaac aaagagattc gcgcgcaaga agttcgcctc 900

accggcgtcg atggcgagca gattggtatt gtcagtctga atgaagctct tgaaaaagct 960

gaggaagcgg gcgtcgattt agtagaaatc agtccgaatg ccgagccgcc agtttgtcga 1020

atctctttag atctctcggt ccgccctgat ggcggcacct tgctgacgtt acgcctgccg 1080

gtacagcagg ttatcaccgg aggcttaaaa tgacccagtt acctaccgcg ggcccggtta 1140

tccggcgctt tgatatgtct gcccagttta cggcgcttta tcgcatcagc gtggcgctga 1200

gtcaggaaag caataccgcg cgcgcactgg cggcgatcct cgaagtgctt cacgatcatg 1260

catttatgca atacggcatg gtgtgtctgt tcgataaaga acgcaatgca ctgtttgtgg 1320

aatccctgca tggcatcgac ggcgaaagga aaaaagaaac ccgccatgtc cgttaccgca 1380

tgggggaagg cgtgatcggc gcggtgatga gccagcgtca ggcgctggtg ttaccgcgca 1440

tttcagacga tcagcgtttt ctcgaccgcc tgaatattta cgattacagc ctgccgctga 1500

ttggtgtgcc gatccccggt gcggataatc agcctgcggg tgtgctggtg gcacagccga 1560

tggcgttgca cgaagaccgg ctggctgcca gtacgcggtt tttagaaatg gtc 1613

<210> 231

<211> 426

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm5

<400> 231

atgaccctga atatgatgat ggatgccggc ggacatcatc gcgacaaaca atattaatac 60

cggcaaccac accggcaatt tacgagactg cgcaggcatc ctttctcccg tcaatttctg 120

tcaaataaag taaaagaggc agtctacttg aattaccccc ggctggttga gcgtttgttg 180

aaaaaaagta actgaaaaat ccgtagaata gcgccactct gatggttaat taacctattc 240

aattaagaat tatctggatg aatgtgccat taaatgcgca gcataatggt gcgttgtgcg 300

ggaaaactgc ttttttttga aagggttggt cagtagcgga aacaactcac ttcacacccc 360

gaagggggaa gttgcctgac cctacgattc ccgctatttc attcactgac cggaggttca 420

aaatga 426

<210> 232

<211> 1426

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm5 with a 500 bp flanking region.

<400> 232

accggatacg agagaaaagt gtctacatcg gttcggttga tattgaccgg cgcatccgcc 60

agcccgccca gtttctggtg gatctgtttg gcgattttgc gggtcttgcc ggtgtcggtg 120

ccgaaaaaaa taccaatatt tgccataaca cacgctcctg ttgaaaaaga gatcccgccg 180

ggaaatgcgg tgaacgtgtc tgatattgcg aagagtgtgc cagttttggt cgcgggcaaa 240

acctgcacca gtttggttat taatgcacca gtctggcgct ttttttcgcc gagtttctcc 300

tcgctaatgc ccgccaggcg cggctttggc gctgatagcg cgctgaatac cgatctggat 360

caaggttttg tcgggttatc agccaaaagg tgcactcttt gcatggttat acgtgcctga 420

catgttgtcc gggcgacaaa cggcctggtg gcacaaattg tcagaactac gacacgacta 480

actgaccgca ggagtgtgcg atgaccctga atatgatgat ggatgccggc ggacatcatc 540

gcgacaaaca atattaatac cggcaaccac accggcaatt tacgagactg cgcaggcatc 600

ctttctcccg tcaatttctg tcaaataaag taaaagaggc agtctacttg aattaccccc 660

ggctggttga gcgtttgttg aaaaaaagta actgaaaaat ccgtagaata gcgccactct 720

gatggttaat taacctattc aattaagaat tatctggatg aatgtgccat taaatgcgca 780

gcataatggt gcgttgtgcg ggaaaactgc ttttttttga aagggttggt cagtagcgga 840

aacaactcac ttcacacccc gaagggggaa gttgcctgac cctacgattc ccgctatttc 900

attcactgac cggaggttca aaatgaccca gcgaaccgag tcgggtaata ccgtctggcg 960

cttcgatttg tcccagcagt tcactgcgat gcagcgcata agcgtggtac tcagccgggc 1020

1080

1140 gcagcacggc atgatctgtc tgtacgacag

gcaggaagcc gatcagcagt taatccccgg cagctcgcaa atccgctatc gtccgggcga 1200

agggctggtc gggacggtgc tttcgcaggg ccaatcatta gtgctggcgc gcgttgctga 1260

1320

gccgctgata gggccagatg cgcagacttt cggtgtgctg acggcacaac ccatggcgcg 1380

ttacgaagag cgattacccg cctgcacccg ctttctggaa acggtc 1426

<210> 233

<211> 461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1

<400> 233

atgaccctga atatgatgat ggatgccggc cgtcctgtaa taataaccgg acaattcgga 60

ctgattaaaa aagcgccctt gtggcgcttt ttttatattc ccgcctccat ttaaaataaa 120

aaatccaatc ggatttcact atttaaactg gccattatct aagatgaatc cgatggaagc 180

240

cgatcaaatt taagtggatt cccatcaaaa aaatattctc aacctaaaaa agtttgtgta 300

atacttgtaa cgctacatgg agattaactc aatctagagg gtattaataa tgaatcgtac 360

taaactggta ctgggcgcaa ctcacttcac accccgaagg gggaagttgc ctgaccctac 420

gattcccgct atttcattca ctgaccggag gttcaaaatg a 461

<210> 234

<211> 1461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1 with a 500 bp flanking region.

<400> 234

accggatacg agagaaaagt gtctacatcg gttcggttga tattgaccgg cgcatccgcc 60

agcccgccca gtttctggtg gatctgtttg gcgattttgc gggtcttgcc ggtgtcggtg 120

ccgaaaaaaa taccaatatt tgccataaca cacgctcctg ttgaaaaaga gatcccgccg 180

ggaaatgcgg tgaacgtgtc tgatattgcg aagagtgtgc cagttttggt cgcgggcaaa 240

acctgcacca gtttggttat taatgcacca gtctggcgct ttttttcgcc gagtttctcc 300

tcgctaatgc ccgccaggcg cggctttggc gctgatagcg cgctgaatac cgatctggat 360

caaggttttg tcgggttatc agccaaaagg tgcactcttt gcatggttat acgtgcctga 420

catgttgtcc gggcgacaaa cggcctggtg gcacaaattg tcagaactac gacacgacta 480

actgaccgca ggagtgtgcg atgaccctga atatgatgat ggatgccggc cgtcctgtaa 540

taataaccgg acaattcggga ctgattaaaa aagcgccctt gtggcgcttt ttttatattc 600

ccgcctccat ttaaaataaa aaatccaatc ggatttcact atttaaactg gccattatct 660

aagatgaatc cgatggaagc tcgctgtttt aacacgcgtt ttttaacctt ttattgaaag 720

tcggtgcttc tttgagcgaa cgatcaaatt taagtggatt cccatcaaaa aaatattctc 780

aacctaaaaa agtttgtgta atacttgtaa cgctacatgg agattaactc aatctagagg 840

gtattaataa tgaatcgtac taaactggta ctgggcgcaa ctcacttcac accccgaagg 900

gggaagttgc ctgaccctac gattcccgct atttcattca ctgaccggag gttcaaaatg 960

acccagcgaa ccgagtcggg taataccgtc tggcgcttcg atttgtccca gcagttcact 1020

gcgatgcagc gcataagcgt ggtactcagc cgggcgaccg aggtcgatca gacgctccag 1080

caagtgctgt gcgtattgca caatgacgcc ttttgcagc acggcatgat ctgtctgtac 1140

gacagccagc aggcgatttt gaatattgaa gcgttgcagg aagccgatca gcagttaatc 1200

cccggcagct cgcaaatccg ctatcgtccg ggcgaagggc tggtcgggac ggtgctttcg 1260

cagggccaat cattagtgct ggcgcgcgtt gctgacgatc agcgctttct tgaccggctc 1320

gggttgtatg attacaacct gccgtttatc gccgtgccgc tgatagggcc agatgcgcag 1380

actttcggtg tgctgacggc acaacccatg gcgcgttacg aagagcgatt acccgcctgc 1440

acccgctttc tggaaacggt c 1461

<210> 235

<211> 461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1

<400> 235

atgaccctga atatgatgat ggatgccggc cgtcctgtaa taataaccgg acaattcgga 60

ctgattaaaa aagcgccctt gtggcgcttt ttttatattc ccgcctccat ttaaaataaa 120

aaatccaatc ggatttcact atttaaactg gccattatct aagatgaatc cgatggaagc 180

240

cgatcaaatt taagtggatt cccatcaaaa aaatattctc aacctaaaaa agtttgtgta 300

atacttgtaa cgctacatgg agattaactc aatctagagg gtattaataa tgaatcgtac 360

taaactggta ctgggcgcaa ctcacttcac accccgaagg gggaagttgc ctgaccctac 420

gattcccgct atttcattca ctgaccggag gttcaaaatg a 461

<210> 236

<211> 1461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1 with a 500 bp flanking region.

<400> 236

accggatacg agagaaaagt gtctacatcg gttcggttga tattgaccgg cgcatccgcc 60

agcccgccca gtttctggtg gatctgtttg gcgattttgc gggtcttgcc ggtgtcggtg 120

ccgaaaaaaa taccaatatt tgccataaca cacgctcctg ttgaaaaaga gatcccgccg 180

ggaaatgcgg tgaacgtgtc tgatattgcg aagagtgtgc cagttttggt cgcgggcaaa 240

acctgcacca gtttggttat taatgcacca gtctggcgct ttttttcgcc gagtttctcc 300

tcgctaatgc ccgccaggcg cggctttggc gctgatagcg cgctgaatac cgatctggat 360

caaggttttg tcgggttatc agccaaaagg tgcactcttt gcatggttat acgtgcctga 420

catgttgtcc gggcgacaaa cggcctggtg gcacaaattg tcagaactac gacacgacta 480

actgaccgca ggagtgtgcg atgaccctga atatgatgat ggatgccggc cgtcctgtaa 540

taataaccgg acaattcggga ctgattaaaa aagcgccctt gtggcgcttt ttttatattc 600

ccgcctccat ttaaaataaa aaatccaatc ggatttcact atttaaactg gccattatct 660

aagatgaatc cgatggaagc tcgctgtttt aacacgcgtt ttttaacctt ttattgaaag 720

tcggtgcttc tttgagcgaa cgatcaaatt taagtggatt cccatcaaaa aaatattctc 780

aacctaaaaa agtttgtgta atacttgtaa cgctacatgg agattaactc aatctagagg 840

gtattaataa tgaatcgtac taaactggta ctgggcgcaa ctcacttcac accccgaagg 900

gggaagttgc ctgaccctac gattcccgct atttcattca ctgaccggag gttcaaaatg 960

acccagcgaa ccgagtcggg taataccgtc tggcgcttcg atttgtccca gcagttcact 1020

gcgatgcagc gcataagcgt ggtactcagc cgggcgaccg aggtcgatca gacgctccag 1080

caagtgctgt gcgtattgca caatgacgcc ttttgcagc acggcatgat ctgtctgtac 1140

gacagccagc aggcgatttt gaatattgaa gcgttgcagg aagccgatca gcagttaatc 1200

cccggcagct cgcaaatccg ctatcgtccg ggcgaagggc tggtcgggac ggtgctttcg 1260

cagggccaat cattagtgct ggcgcgcgtt gctgacgatc agcgctttct tgaccggctc 1320

gggttgtatg attacaacct gccgtttatc gccgtgccgc tgatagggcc agatgcgcag 1380

actttcggtg tgctgacggc acaacccatg gcgcgttacg aagagcgatt acccgcctgc 1440

acccgctttc tggaaacggt c 1461

<210> 237

<211> 1188

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2

<400> 237

atggcactga aacacctcat ttccctgtgt gccgcgtcgc cgatggttgc cagtcagctg 60

gcgcgctacc cgatcctgct tgatgaattg ctcgacccga atacgctcta tcaaccgacg 120

gcgatgaatg cctatcgcga tgagctgcgc caatacctgc tgcgcgtgcc ggaagatgat 180

gaagagcaac agcttgaggc gctgcggcag tttaagcagg cgcagttgct gcgcgtggcg 240

gcggcggata ttgccggtac gttgccagta atgaaagtga gcgatcactt aacctggctg 300

360

ggccagccaa cgcatctgca cgatcgcgaa gggcgcggtt ttgcggtggt cggttatggc 420

aagctgggcg gctgggagct gggttacagc tccgatctgg atctggtatt cctgcacgac 480

tgcccgatgg atgtgatgac cgatggcgag cgtgaaatcg atggtcgcca gttctatttg 540

cgtctcgcgc agcgcgtgat gcacctgttt agcacgcgca cgtcgtccgg catcctttat 600

gaagttgatg cgcgtctgcg tccatctggc gctgcgggga tgctggtcac tactacggaa 660

tcgttcgccg attaccagca aaacgaagcc tggacgtggg aacatcaggc gctggcccgt 720

gcgcgcgtgg tgtacggcga tccgcaactg accgccgaat ttgacgccat tcgccgcgat 780

attctgatga cgcctcgcga cggcgcaacg ctgcaaaccg acgtgcgaga aatgcgcgag 840

aaaatgcgtg cccatcttgg caacaagcat aaagaccgct tcgatctgaa agccgatgaa 900

ggcggtatca ccgacatcga gtttatcgcc caatatctgg tgctgcgctt tgcccatgac 960

aagccgaaac tgacgcgctg gtcggataat gtgcgcattc tcgaagggct ggcgcaaaac 1020

ggcatcatgg aggagcagga agcgcaggca ttgacgctgg cgtacaccac attgcgtgat 1080

gagctgcacc acctggcgct gcaagagttg ccgggacatg tggcgctctc ctgttttgtc 1140

gccgagcgtg cgcttattaa aaccagctgg gacaagtggc tggtggaa 1188

<210> 238

<211> 2206

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2 with a 500 bp flanking region.

<400> 238

gcgcaaagcg agtgctcact tacgtgatct gttgacacaa tctgaagcga ccataacttc 60

tgccgtttca gcgaatacgg cggtgtggag cgcacaatca gccctggcga agctggtgct 120

caccgagtgg ctagtgacgc agggctggcg aaccttcctt gatgaaaaag cgcaggccaa 180

attcgccgac tcctttaaac gctttgctga catccatctg tcacgcagcg ccgccgagct 240

gaaaaaagcc tttgcccaac cgctgggcga cagctatcgc gaccagttgc cgcgcctggc 300

gcgtgatatc gactgcgcgt tactgctggc cgggcattac gatcgcgcgc gcgccgtgga 360

atggctggaa aactggcagg ggcttcagca cgccattgaa acgcgccaga gagtcgaaat 420

cgaacatttc cgtaataccg cgattaccca ggagccgttc tggttgcaca gcggaaaacg 480

ttaacgaaag gatatttcgc atggcactga aacacctcat ttccctgtgt gccgcgtcgc 540

cgatggttgc cagtcagctg gcgcgctacc cgatcctgct tgatgaattg ctcgacccga 600

atacgctcta tcaaccgacg gcgatgaatg cctatcgcga tgagctgcgc caatacctgc 660

tgcgcgtgcc ggaagatgat gaagagcaac agcttgaggc gctgcggcag tttaagcagg 720

cgcagttgct gcgcgtggcg gcggcggata ttgccggtac gttgccagta atgaaagtga 780

gcgatcactt aacctggctg gcggaagcga ttattgatgc ggtggtgcag caagcctggg 840

ggcagatggt ggcgcgttat ggccagccaa cgcatctgca cgatcgcgaa gggcgcggtt 900

ttgcggtggt cggttatggc aagctgggcg gctgggagct gggttacagc tccgatctgg 960

atctggtatt cctgcacgac tgcccgatgg atgtgatgac cgatggcgag cgtgaaatcg 1020

atggtcgcca gttctatttg cgtctcgcgc agcgcgtgat gcacctgttt agcacgcgca 1080

cgtcgtccgg catcctttat gaagttgatg cgcgtctgcg tccatctggc gctgcgggga 1140

tgctggtcac tactacggaa tcgttcgccg attaccagca aaacgaagcc tggacgtggg 1200

aacatcaggc gctggcccgt gcgcgcgtgg tgtacggcga tccgcaactg accgccgaat 1260

ttgacgccat tcgccgcgat attctgatga cgcctcgcga cggcgcaacg ctgcaaaccg 1320

acgtgcgaga aatgcgcgag aaaatgcgtg cccatcttgg caacaagcat aaagaccgct 1380

tcgatctgaa agccgatgaa ggcggtatca ccgacatcga gtttatcgcc caatatctgg 1440

tgctgcgctt tgcccatgac aagccgaaac tgacgcgctg gtcggataat gtgcgcattc 1500

tcgaagggct ggcgcaaaac ggcatcatgg aggagcagga agcgcaggca ttgacgctgg 1560

cgtacaccac attgcgtgat gagctgcacc acctggcgct gcaagagttg ccgggacatg 1620

tggcgctctc ctgttttgtc gccgagcgtg cgcttattaa aaccagctgg gacaagtggc 1680

tggtggaacc gtgcgccccg gcgtaagtgt ggtatcatcg cgcgcaaatt ttgtatctct 1740

caggagacag gaatgaaagt gacgctgcca gagtttaagc aagccggtgt aatggtggtg 1800

ggtgatgtga tgctggatcg ttactggtat ggcccaacca gccgtatctc tcgggaagcg 1860

ccagtcccgg ttgttaaagt cgataccatt gaagagcgtc ctggcggcgc ggcaaacgtg 1920

gcgatgaata tcgcctcact gggcgccacg gcgcgtctgg ttggcctgac tggcattgac 1980

gatgcggcgc gcgcgctgag caaagcgctg gccgatgtta acgttaaatg tgacttcgtt 2040

tctgttccga cgcatcccac catcactaag ctgcgcgtgc tgtcgcgtaa ccagcagctg 2100

attcgcctgg actttgaaga gggttttgaa ggagtcgatc cgcaaccgat gcatgaacgc 2160

atcagccagg cgcttggtaa tattggcgcg ctggtgctgt cggatt 2206

<210> 239

<211> 1563

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-1

<400> 239

atgtttaacg atctgattgg cgatgatgaa acggattcgc cggaagatgc gctttctgag 60

agctggcgcg aattgtggca ggatgcgttg caggaggagg attccacgcc cgtgctggcg 120

catctctcag aggacgatcg ccgccgcgtg gtggcgctga ttgccgattt tcgcaaagag 180

ttggataaac gcaccattgg cccgcgaggg cggcaggtac tcgatcactt aatgccgcat 240

ctgctcagcg atgtatgctc gcgcgacgat gcgccagtac cgctgtcacg cctgacgccg 300

ctgctcaccg gaattattac ccgcaccact taccttgagc tgctaagtga atttcccggc 360

gcactgaaac acctcatttc cctgtgtgcc gcgtcgccga tggttgccag tcagctggcg 420

cgctacccga tcctgcttga tgaattgctc gacccgaata cgctctatca accgacggcg 480

atgaatgcct atcgcgatga gctgcgccaa tacctgctgc gcgtgccgga agatgatgaa 540

gagcaacagc ttgaggcgct gcggcagttt aagcaggcgc agttgctgcg cgtggcggcg 600

gcggatattg ccggtacgtt gccagtaatg aaagtgagcg atcacttaac ctggctggcg 660

gaagcgatta ttgatgcggt ggtgcagcaa gcctgggggc agatggtggc gcgttatggc 720

cagccaacgc atctgcacga tcgcgaaggg cgcggttttg cggtggtcgg ttatggcaag 780

ctgggcggct gggagctggg ttacagctcc gatctggatc tggtattcct gcacgactgc 840

ccgatggatg tgatgaccga tggcgagcgt gaaatcgatg gtcgccagtt ctatttgcgt 900

ctcgcgcagc gcgtgatgca cctgtttagc acgcgcacgt cgtccggcat cctttatgaa 960

gttgatgcgc gtctgcgtcc atctggcgct gcggggatgc tggtcactac tacggaatcg 1020

ttcgccgatt accagcaaaa cgaagcctgg acgtgggaac atcaggcgct ggcccgtgcg 1080

cgcgtggtgt acggcgatcc gcaactgacc gccgaatttg acgccattcg ccgcgatatt 1140

ctgatgacgc ctcgcgacgg cgcaacgctg caaaccgacg tgcgagaaat gcgcgagaaa 1200

atgcgtgccc atcttggcaa caagcataaa gaccgcttcg atctgaaagc cgatgaaggc 1260

ggtatcaccg acatcgagtt tatcgcccaa tatctggtgc tgcgctttgc ccatgacaag 1320

ccgaaactga cgcgctggtc ggataatgtg cgcattctcg aagggctggc gcaaaacggc 1380

atcatggagg agcaggaagc gcaggcattg acgctggcgt acaccacatt gcgtgatgag 1440

ctgcaccacc tggcgctgca agagttgccg ggacatgtgg cgctctcctg ttttgtcgcc 1500

1560 gagcgtgcgc ttattaaaac cagctgggac

taa 1563

<210> 240

<211> 461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1

<400> 240

atgaccctga atatgatgat ggatgccggc cgtcctgtaa taataaccgg acaattcgga 60

ctgattaaaa aagcgccctt gtggcgcttt ttttatattc ccgcctccat ttaaaataaa 120

aaatccaatc ggatttcact atttaaactg gccattatct aagatgaatc cgatggaagc 180

240

cgatcaaatt taagtggatt cccatcaaaa aaatattctc aacctaaaaa agtttgtgta 300

atacttgtaa cgctacatgg agattaactc aatctagagg gtattaataa tgaatcgtac 360

taaactggta ctgggcgcaa ctcacttcac accccgaagg gggaagttgc ctgaccctac 420

gattcccgct atttcattca ctgaccggag gttcaaaatg a 461

<210> 241

<211> 1461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1 with a 500 bp flanking region.

<400> 241

accggatacg agagaaaagt gtctacatcg gttcggttga tattgaccgg cgcatccgcc 60

agcccgccca gtttctggtg gatctgtttg gcgattttgc gggtcttgcc ggtgtcggtg 120

ccgaaaaaaa taccaatatt tgccataaca cacgctcctg ttgaaaaaga gatcccgccg 180

ggaaatgcgg tgaacgtgtc tgatattgcg aagagtgtgc cagttttggt cgcgggcaaa 240

acctgcacca gtttggttat taatgcacca gtctggcgct ttttttcgcc gagtttctcc 300

tcgctaatgc ccgccaggcg cggctttggc gctgatagcg cgctgaatac cgatctggat 360

caaggttttg tcgggttatc agccaaaagg tgcactcttt gcatggttat acgtgcctga 420

catgttgtcc gggcgacaaa cggcctggtg gcacaaattg tcagaactac gacacgacta 480

actgaccgca ggagtgtgcg atgaccctga atatgatgat ggatgccggc cgtcctgtaa 540

taataaccgg acaattcggga ctgattaaaa aagcgccctt gtggcgcttt ttttatattc 600

ccgcctccat ttaaaataaa aaatccaatc ggatttcact atttaaactg gccattatct 660

aagatgaatc cgatggaagc tcgctgtttt aacacgcgtt ttttaacctt ttattgaaag 720

tcggtgcttc tttgagcgaa cgatcaaatt taagtggatt cccatcaaaa aaatattctc 780

aacctaaaaa agtttgtgta atacttgtaa cgctacatgg agattaactc aatctagagg 840

gtattaataa tgaatcgtac taaactggta ctgggcgcaa ctcacttcac accccgaagg 900

gggaagttgc ctgaccctac gattcccgct atttcattca ctgaccggag gttcaaaatg 960

acccagcgaa ccgagtcggg taataccgtc tggcgcttcg atttgtccca gcagttcact 1020

gcgatgcagc gcataagcgt ggtactcagc cgggcgaccg aggtcgatca gacgctccag 1080

caagtgctgt gcgtattgca caatgacgcc ttttgcagc acggcatgat ctgtctgtac 1140

gacagccagc aggcgatttt gaatattgaa gcgttgcagg aagccgatca gcagttaatc 1200

cccggcagct cgcaaatccg ctatcgtccg ggcgaagggc tggtcgggac ggtgctttcg 1260

cagggccaat cattagtgct ggcgcgcgtt gctgacgatc agcgctttct tgaccggctc 1320

gggttgtatg attacaacct gccgtttatc gccgtgccgc tgatagggcc agatgcgcag 1380

actttcggtg tgctgacggc acaacccatg gcgcgttacg aagagcgatt acccgcctgc 1440

acccgctttc tggaaacggt c 1461

<210> 242

<211> 2563

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-1 with a 500 bp flanking region.

<400> 242

gcgcaaagcg agtgctcact tacgtgatct gttgacacaa tctgaagcga ccataacttc 60

tgccgtttca gcgaatacgg cggtgtggag cgcacaatca gccctggcga agctggtgct 120

caccgagtgg ctagtgacgc agggctggcg aaccttcctt gatgaaaaag cgcaggccaa 180

attcgccgac tcctttaaac gctttgctga catccatctg tcacgcagcg ccgccgagct 240

gaaaaaagcc tttgcccaac cgctgggcga cagctatcgc gaccagttgc cgcgcctggc 300

gcgtgatatc gactgcgcgt tactgctggc cgggcattac gatcgcgcgc gcgccgtgga 360

atggctggaa aactggcagg ggcttcagca cgccattgaa acgcgccaga gagtcgaaat 420

cgaacatttc cgtaataccg cgattaccca ggagccgttc tggttgcaca gcggaaaacg 480

ttaacgaaag gatatttcgc atgtttaacg atctgattgg cgatgatgaa acggattcgc 540

cggaagatgc gctttctgag agctggcgcg aattgtggca ggatgcgttg caggaggagg 600

attccacgcc cgtgctggcg catctctcag aggacgatcg ccgccgcgtg gtggcgctga 660

ttgccgattt tcgcaaagag ttggataaac gcaccattgg cccgcgaggg cggcaggtac 720

tcgatcactt aatgccgcat ctgctcagcg atgtatgctc gcgcgacgat gcgccagtac 780

cgctgtcacg cctgacgccg ctgctcaccg gaattattac ccgcaccact taccttgagc 840

tgctaagtga atttcccggc gcactgaaac acctcatttc cctgtgtgcc gcgtcgccga 900

tggttgccag tcagctggcg cgctacccga tcctgcttga tgaattgctc gacccgaata 960

cgctctatca accgacggcg atgaatgcct atcgcgatga gctgcgccaa tacctgctgc 1020

gcgtgccgga agatgatgaa gagcaacagc ttgaggcgct gcggcagttt aagcaggcgc 1080

agttgctgcg cgtggcggcg gcggatattg ccggtacgtt gccagtaatg aaagtgagcg 1140

atcacttaac ctggctggcg gaagcgatta ttgatgcggt ggtgcagcaa gcctgggggc 1200

agatggtggc gcgttatggc cagccaacgc atctgcacga tcgcgaaggg cgcggttttg 1260

cggtggtcgg ttatggcaag ctgggcggct gggagctggg ttacagctcc gatctggatc 1320

tggtattcct gcacgactgc ccgatggatg tgatgaccga tggcgagcgt gaaatcgatg 1380

gtcgccagtt ctatttgcgt ctcgcgcagc gcgtgatgca cctgtttagc acgcgcacgt 1440

cgtccggcat cctttatgaa gttgatgcgc gtctgcgtcc atctggcgct gcggggatgc 1500

tggtcactac tacggaatcg ttcgccgatt accagcaaaa cgaagcctgg acgtgggaac 1560

atcaggcgct ggcccgtgcg cgcgtggtgt acggcgatcc gcaactgacc gccgaatttg 1620

acgccattcg ccgcgatatt ctgatgacgc ctcgcgacgg cgcaacgctg caaaccgacg 1680

tgcgagaaat gcgcgagaaa atgcgtgccc atcttggcaa caagcataaa gaccgcttcg 1740

atctgaaagc cgatgaaggc ggtatcaccg acatcgagtt tatcgcccaa tatctggtgc 1800

tgcgctttgc ccatgacaag ccgaaactga cgcgctggtc ggataatgtg cgcattctcg 1860

aagggctggc gcaaaacggc atcatggagg agcaggaagc gcaggcattg acgctggcgt 1920

acaccacatt gcgtgatgag ctgcaccacc tggcgctgca agagttgccg ggacatgtgg 1980

cgctctcctg ttttgtcgcc gagcgtgcgc ttattaaaac cagctgggac aagtggctgg 2040

tggaaccgtg cgccccggcg taagtgtggt atcatcgcgc gcaaattttg tatctctcag 2100

gagacaggaa tgaaagtgac gctgccagag tttaagcaag ccggtgtaat ggtggtgggt 2160

gatgtgatgc tggatcgtta ctggtatggc ccaaccagcc gtatctctcc ggaagcgcca 2220

gtcccggttg ttaaagtcga taccattgaa gagcgtcctg gcggcgcggc aaacgtggcg 2280

atgaatatcg cctcactggg cgccacggcg cgtctggttg gcctgactgg cattgacgat 2340

gcggcgcgcg cgctgagcaa agcgctggcc gatgttaacg ttaaatgtga cttcgtttct 2400

gttccgacgc atcccaccat cactaagctg cgcgtgctgt cgcgtaacca gcagctgatt 2460

cgcctggact ttgaagaggg ttttgaagga gtcgatccgc aaccgatgca tgaacgcatc 2520

agccaggcgc ttggtaatat tggcgcgctg gtgctgtcgg att 2563

<210> 243

<211> 1563

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-1

<400> 243

atgtttaacg atctgattgg cgatgatgaa acggattcgc cggaagatgc gctttctgag 60

agctggcgcg aattgtggca ggatgcgttg caggaggagg attccacgcc cgtgctggcg 120

catctctcag aggacgatcg ccgccgcgtg gtggcgctga ttgccgattt tcgcaaagag 180

ttggataaac gcaccattgg cccgcgaggg cggcaggtac tcgatcactt aatgccgcat 240

ctgctcagcg atgtatgctc gcgcgacgat gcgccagtac cgctgtcacg cctgacgccg 300

ctgctcaccg gaattattac ccgcaccact taccttgagc tgctaagtga atttcccggc 360

gcactgaaac acctcatttc cctgtgtgcc gcgtcgccga tggttgccag tcagctggcg 420

cgctacccga tcctgcttga tgaattgctc gacccgaata cgctctatca accgacggcg 480

atgaatgcct atcgcgatga gctgcgccaa tacctgctgc gcgtgccgga agatgatgaa 540

gagcaacagc ttgaggcgct gcggcagttt aagcaggcgc agttgctgcg cgtggcggcg 600

gcggatattg ccggtacgtt gccagtaatg aaagtgagcg atcacttaac ctggctggcg 660

gaagcgatta ttgatgcggt ggtgcagcaa gcctgggggc agatggtggc gcgttatggc 720

cagccaacgc atctgcacga tcgcgaaggg cgcggttttg cggtggtcgg ttatggcaag 780

ctgggcggct gggagctggg ttacagctcc gatctggatc tggtattcct gcacgactgc 840

ccgatggatg tgatgaccga tggcgagcgt gaaatcgatg gtcgccagtt ctatttgcgt 900

ctcgcgcagc gcgtgatgca cctgtttagc acgcgcacgt cgtccggcat cctttatgaa 960

gttgatgcgc gtctgcgtcc atctggcgct gcggggatgc tggtcactac tacggaatcg 1020

ttcgccgatt accagcaaaa cgaagcctgg acgtgggaac atcaggcgct ggcccgtgcg 1080

cgcgtggtgt acggcgatcc gcaactgacc gccgaatttg acgccattcg ccgcgatatt 1140

ctgatgacgc ctcgcgacgg cgcaacgctg caaaccgacg tgcgagaaat gcgcgagaaa 1200

atgcgtgccc atcttggcaa caagcataaa gaccgcttcg atctgaaagc cgatgaaggc 1260

ggtatcaccg acatcgagtt tatcgcccaa tatctggtgc tgcgctttgc ccatgacaag 1320

ccgaaactga cgcgctggtc ggataatgtg cgcattctcg aagggctggc gcaaaacggc 1380

atcatggagg agcaggaagc gcaggcattg acgctggcgt acaccacatt gcgtgatgag 1440

ctgcaccacc tggcgctgca agagttgccg ggacatgtgg cgctctcctg ttttgtcgcc 1500

1560 gagcgtgcgc ttattaaaac cagctgggac

taa 1563

<210> 244

<211> 2563

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-1 with a 500 bp flanking region.

<400> 244

gcgcaaagcg agtgctcact tacgtgatct gttgacacaa tctgaagcga ccataacttc 60

tgccgtttca gcgaatacgg cggtgtggag cgcacaatca gccctggcga agctggtgct 120

caccgagtgg ctagtgacgc agggctggcg aaccttcctt gatgaaaaag cgcaggccaa 180

attcgccgac tcctttaaac gctttgctga catccatctg tcacgcagcg ccgccgagct 240

gaaaaaagcc tttgcccaac cgctgggcga cagctatcgc gaccagttgc cgcgcctggc 300

gcgtgatatc gactgcgcgt tactgctggc cgggcattac gatcgcgcgc gcgccgtgga 360

atggctggaa aactggcagg ggcttcagca cgccattgaa acgcgccaga gagtcgaaat 420

cgaacatttc cgtaataccg cgattaccca ggagccgttc tggttgcaca gcggaaaacg 480

ttaacgaaag gatatttcgc atgtttaacg atctgattgg cgatgatgaa acggattcgc 540

cggaagatgc gctttctgag agctggcgcg aattgtggca ggatgcgttg caggaggagg 600

attccacgcc cgtgctggcg catctctcag aggacgatcg ccgccgcgtg gtggcgctga 660

ttgccgattt tcgcaaagag ttggataaac gcaccattgg cccgcgaggg cggcaggtac 720

tcgatcactt aatgccgcat ctgctcagcg atgtatgctc gcgcgacgat gcgccagtac 780

cgctgtcacg cctgacgccg ctgctcaccg gaattattac ccgcaccact taccttgagc 840

tgctaagtga atttcccggc gcactgaaac acctcatttc cctgtgtgcc gcgtcgccga 900

tggttgccag tcagctggcg cgctacccga tcctgcttga tgaattgctc gacccgaata 960

cgctctatca accgacggcg atgaatgcct atcgcgatga gctgcgccaa tacctgctgc 1020

gcgtgccgga agatgatgaa gagcaacagc ttgaggcgct gcggcagttt aagcaggcgc 1080

agttgctgcg cgtggcggcg gcggatattg ccggtacgtt gccagtaatg aaagtgagcg 1140

atcacttaac ctggctggcg gaagcgatta ttgatgcggt ggtgcagcaa gcctgggggc 1200

agatggtggc gcgttatggc cagccaacgc atctgcacga tcgcgaaggg cgcggttttg 1260

cggtggtcgg ttatggcaag ctgggcggct gggagctggg ttacagctcc gatctggatc 1320

tggtattcct gcacgactgc ccgatggatg tgatgaccga tggcgagcgt gaaatcgatg 1380

gtcgccagtt ctatttgcgt ctcgcgcagc gcgtgatgca cctgtttagc acgcgcacgt 1440

cgtccggcat cctttatgaa gttgatgcgc gtctgcgtcc atctggcgct gcggggatgc 1500

tggtcactac tacggaatcg ttcgccgatt accagcaaaa cgaagcctgg acgtgggaac 1560

atcaggcgct ggcccgtgcg cgcgtggtgt acggcgatcc gcaactgacc gccgaatttg 1620

acgccattcg ccgcgatatt ctgatgacgc ctcgcgacgg cgcaacgctg caaaccgacg 1680

tgcgagaaat gcgcgagaaa atgcgtgccc atcttggcaa caagcataaa gaccgcttcg 1740

atctgaaagc cgatgaaggc ggtatcaccg acatcgagtt tatcgcccaa tatctggtgc 1800

tgcgctttgc ccatgacaag ccgaaactga cgcgctggtc ggataatgtg cgcattctcg 1860

aagggctggc gcaaaacggc atcatggagg agcaggaagc gcaggcattg acgctggcgt 1920

acaccacatt gcgtgatgag ctgcaccacc tggcgctgca agagttgccg ggacatgtgg 1980

cgctctcctg ttttgtcgcc gagcgtgcgc ttattaaaac cagctgggac aagtggctgg 2040

tggaaccgtg cgccccggcg taagtgtggt atcatcgcgc gcaaattttg tatctctcag 2100

gagacaggaa tgaaagtgac gctgccagag tttaagcaag ccggtgtaat ggtggtgggt 2160

gatgtgatgc tggatcgtta ctggtatggc ccaaccagcc gtatctctcc ggaagcgcca 2220

gtcccggttg ttaaagtcga taccattgaa gagcgtcctg gcggcgcggc aaacgtggcg 2280

atgaatatcg cctcactggg cgccacggcg cgtctggttg gcctgactgg cattgacgat 2340

gcggcgcgcg cgctgagcaa agcgctggcc gatgttaacg ttaaatgtga cttcgtttct 2400

gttccgacgc atcccaccat cactaagctg cgcgtgctgt cgcgtaacca gcagctgatt 2460

cgcctggact ttgaagaggg ttttgaagga gtcgatccgc aaccgatgca tgaacgcatc 2520

agccaggcgc ttggtaatat tggcgcgctg gtgctgtcgg att 2563

<210> 245

<211> 426

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm5

<400> 245

atgaccctga atatgatgat ggatgccggc ggacatcatc gcgacaaaca atattaatac 60

cggcaaccac accggcaatt tacgagactg cgcaggcatc ctttctcccg tcaatttctg 120

tcaaataaag taaaagaggc agtctacttg aattaccccc ggctggttga gcgtttgttg 180

aaaaaaagta actgaaaaat ccgtagaata gcgccactct gatggttaat taacctattc 240

aattaagaat tatctggatg aatgtgccat taaatgcgca gcataatggt gcgttgtgcg 300

ggaaaactgc ttttttttga aagggttggt cagtagcgga aacaactcac ttcacacccc 360

gaagggggaa gttgcctgac cctacgattc ccgctatttc attcactgac cggaggttca 420

aaatga 426

<210> 246

<211> 1426

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm5 with a 500 bp flanking region.

<400> 246

accggatacg agagaaaagt gtctacatcg gttcggttga tattgaccgg cgcatccgcc 60

agcccgccca gtttctggtg gatctgtttg gcgattttgc gggtcttgcc ggtgtcggtg 120

ccgaaaaaaa taccaatatt tgccataaca cacgctcctg ttgaaaaaga gatcccgccg 180

ggaaatgcgg tgaacgtgtc tgatattgcg aagagtgtgc cagttttggt cgcgggcaaa 240

acctgcacca gtttggttat taatgcacca gtctggcgct ttttttcgcc gagtttctcc 300

tcgctaatgc ccgccaggcg cggctttggc gctgatagcg cgctgaatac cgatctggat 360

caaggttttg tcgggttatc agccaaaagg tgcactcttt gcatggttat acgtgcctga 420

catgttgtcc gggcgacaaa cggcctggtg gcacaaattg tcagaactac gacacgacta 480

actgaccgca ggagtgtgcg atgaccctga atatgatgat ggatgccggc ggacatcatc 540

gcgacaaaca atattaatac cggcaaccac accggcaatt tacgagactg cgcaggcatc 600

ctttctcccg tcaatttctg tcaaataaag taaaagaggc agtctacttg aattaccccc 660

ggctggttga gcgtttgttg aaaaaaagta actgaaaaat ccgtagaata gcgccactct 720

gatggttaat taacctattc aattaagaat tatctggatg aatgtgccat taaatgcgca 780

gcataatggt gcgttgtgcg ggaaaactgc ttttttttga aagggttggt cagtagcgga 840

aacaactcac ttcacacccc gaagggggaa gttgcctgac cctacgattc ccgctatttc 900

attcactgac cggaggttca aaatgaccca gcgaaccgag tcgggtaata ccgtctggcg 960

cttcgatttg tcccagcagt tcactgcgat gcagcgcata agcgtggtac tcagccgggc 1020

1080

1140 gcagcacggc atgatctgtc tgtacgacag

gcaggaagcc gatcagcagt taatccccgg cagctcgcaa atccgctatc gtccgggcga 1200

agggctggtc gggacggtgc tttcgcaggg ccaatcatta gtgctggcgc gcgttgctga 1260

1320

gccgctgata gggccagatg cgcagacttt cggtgtgctg acggcacaac ccatggcgcg 1380

ttacgaagag cgattacccg cctgcacccg ctttctggaa acggtc 1426

<210> 247

<211> 461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1

<400> 247

atgaccctga atatgatgat ggatgccggc cgtcctgtaa taataaccgg acaattcgga 60

ctgattaaaa aagcgccctt gtggcgcttt ttttatattc ccgcctccat ttaaaataaa 120

aaatccaatc ggatttcact atttaaactg gccattatct aagatgaatc cgatggaagc 180

240

cgatcaaatt taagtggatt cccatcaaaa aaatattctc aacctaaaaa agtttgtgta 300

atacttgtaa cgctacatgg agattaactc aatctagagg gtattaataa tgaatcgtac 360

taaactggta ctgggcgcaa ctcacttcac accccgaagg gggaagttgc ctgaccctac 420

gattcccgct atttcattca ctgaccggag gttcaaaatg a 461

<210> 248

<211> 1461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1 with a 500 bp flanking region.

<400> 248

accggatacg agagaaaagt gtctacatcg gttcggttga tattgaccgg cgcatccgcc 60

agcccgccca gtttctggtg gatctgtttg gcgattttgc gggtcttgcc ggtgtcggtg 120

ccgaaaaaaa taccaatatt tgccataaca cacgctcctg ttgaaaaaga gatcccgccg 180

ggaaatgcgg tgaacgtgtc tgatattgcg aagagtgtgc cagttttggt cgcgggcaaa 240

acctgcacca gtttggttat taatgcacca gtctggcgct ttttttcgcc gagtttctcc 300

tcgctaatgc ccgccaggcg cggctttggc gctgatagcg cgctgaatac cgatctggat 360

caaggttttg tcgggttatc agccaaaagg tgcactcttt gcatggttat acgtgcctga 420

catgttgtcc gggcgacaaa cggcctggtg gcacaaattg tcagaactac gacacgacta 480

actgaccgca ggagtgtgcg atgaccctga atatgatgat ggatgccggc cgtcctgtaa 540

taataaccgg acaattcggga ctgattaaaa aagcgccctt gtggcgcttt ttttatattc 600

ccgcctccat ttaaaataaa aaatccaatc ggatttcact atttaaactg gccattatct 660

aagatgaatc cgatggaagc tcgctgtttt aacacgcgtt ttttaacctt ttattgaaag 720

tcggtgcttc tttgagcgaa cgatcaaatt taagtggatt cccatcaaaa aaatattctc 780

aacctaaaaa agtttgtgta atacttgtaa cgctacatgg agattaactc aatctagagg 840

gtattaataa tgaatcgtac taaactggta ctgggcgcaa ctcacttcac accccgaagg 900

gggaagttgc ctgaccctac gattcccgct atttcattca ctgaccggag gttcaaaatg 960

acccagcgaa ccgagtcggg taataccgtc tggcgcttcg atttgtccca gcagttcact 1020

gcgatgcagc gcataagcgt ggtactcagc cgggcgaccg aggtcgatca gacgctccag 1080

caagtgctgt gcgtattgca caatgacgcc ttttgcagc acggcatgat ctgtctgtac 1140

gacagccagc aggcgatttt gaatattgaa gcgttgcagg aagccgatca gcagttaatc 1200

cccggcagct cgcaaatccg ctatcgtccg ggcgaagggc tggtcgggac ggtgctttcg 1260

cagggccaat cattagtgct ggcgcgcgtt gctgacgatc agcgctttct tgaccggctc 1320

gggttgtatg attacaacct gccgtttatc gccgtgccgc tgatagggcc agatgcgcag 1380

actttcggtg tgctgacggc acaacccatg gcgcgttacg aagagcgatt acccgcctgc 1440

acccgctttc tggaaacggt c 1461

<210> 249

<211> 1188

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2

<400> 249

atggcactga aacacctcat ttccctgtgt gccgcgtcgc cgatggttgc cagtcagctg 60

gcgcgctacc cgatcctgct tgatgaattg ctcgacccga atacgctcta tcaaccgacg 120

gcgatgaatg cctatcgcga tgagctgcgc caatacctgc tgcgcgtgcc ggaagatgat 180

gaagagcaac agcttgaggc gctgcggcag tttaagcagg cgcagttgct gcgcgtggcg 240

gcggcggata ttgccggtac gttgccagta atgaaagtga gcgatcactt aacctggctg 300

360

ggccagccaa cgcatctgca cgatcgcgaa gggcgcggtt ttgcggtggt cggttatggc 420

aagctgggcg gctgggagct gggttacagc tccgatctgg atctggtatt cctgcacgac 480

tgcccgatgg atgtgatgac cgatggcgag cgtgaaatcg atggtcgcca gttctatttg 540

cgtctcgcgc agcgcgtgat gcacctgttt agcacgcgca cgtcgtccgg catcctttat 600

gaagttgatg cgcgtctgcg tccatctggc gctgcgggga tgctggtcac tactacggaa 660

tcgttcgccg attaccagca aaacgaagcc tggacgtggg aacatcaggc gctggcccgt 720

gcgcgcgtgg tgtacggcga tccgcaactg accgccgaat ttgacgccat tcgccgcgat 780

attctgatga cgcctcgcga cggcgcaacg ctgcaaaccg acgtgcgaga aatgcgcgag 840

aaaatgcgtg cccatcttgg caacaagcat aaagaccgct tcgatctgaa agccgatgaa 900

ggcggtatca ccgacatcga gtttatcgcc caatatctgg tgctgcgctt tgcccatgac 960

aagccgaaac tgacgcgctg gtcggataat gtgcgcattc tcgaagggct ggcgcaaaac 1020

ggcatcatgg aggagcagga agcgcaggca ttgacgctgg cgtacaccac attgcgtgat 1080

gagctgcacc acctggcgct gcaagagttg ccgggacatg tggcgctctc ctgttttgtc 1140

gccgagcgtg cgcttattaa aaccagctgg gacaagtggc tggtggaa 1188

<210> 250

<211> 2206

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2 with a 500 bp flanking region.

<400> 250

gcgcaaagcg agtgctcact tacgtgatct gttgacacaa tctgaagcga ccataacttc 60

tgccgtttca gcgaatacgg cggtgtggag cgcacaatca gccctggcga agctggtgct 120

caccgagtgg ctagtgacgc agggctggcg aaccttcctt gatgaaaaag cgcaggccaa 180

attcgccgac tcctttaaac gctttgctga catccatctg tcacgcagcg ccgccgagct 240

gaaaaaagcc tttgcccaac cgctgggcga cagctatcgc gaccagttgc cgcgcctggc 300

gcgtgatatc gactgcgcgt tactgctggc cgggcattac gatcgcgcgc gcgccgtgga 360

atggctggaa aactggcagg ggcttcagca cgccattgaa acgcgccaga gagtcgaaat 420

cgaacatttc cgtaataccg cgattaccca ggagccgttc tggttgcaca gcggaaaacg 480

ttaacgaaag gatatttcgc atggcactga aacacctcat ttccctgtgt gccgcgtcgc 540

cgatggttgc cagtcagctg gcgcgctacc cgatcctgct tgatgaattg ctcgacccga 600

atacgctcta tcaaccgacg gcgatgaatg cctatcgcga tgagctgcgc caatacctgc 660

tgcgcgtgcc ggaagatgat gaagagcaac agcttgaggc gctgcggcag tttaagcagg 720

cgcagttgct gcgcgtggcg gcggcggata ttgccggtac gttgccagta atgaaagtga 780

gcgatcactt aacctggctg gcggaagcga ttattgatgc ggtggtgcag caagcctggg 840

ggcagatggt ggcgcgttat ggccagccaa cgcatctgca cgatcgcgaa gggcgcggtt 900

ttgcggtggt cggttatggc aagctgggcg gctgggagct gggttacagc tccgatctgg 960

atctggtatt cctgcacgac tgcccgatgg atgtgatgac cgatggcgag cgtgaaatcg 1020

atggtcgcca gttctatttg cgtctcgcgc agcgcgtgat gcacctgttt agcacgcgca 1080

cgtcgtccgg catcctttat gaagttgatg cgcgtctgcg tccatctggc gctgcgggga 1140

tgctggtcac tactacggaa tcgttcgccg attaccagca aaacgaagcc tggacgtggg 1200

aacatcaggc gctggcccgt gcgcgcgtgg tgtacggcga tccgcaactg accgccgaat 1260

ttgacgccat tcgccgcgat attctgatga cgcctcgcga cggcgcaacg ctgcaaaccg 1320

acgtgcgaga aatgcgcgag aaaatgcgtg cccatcttgg caacaagcat aaagaccgct 1380

tcgatctgaa agccgatgaa ggcggtatca ccgacatcga gtttatcgcc caatatctgg 1440

tgctgcgctt tgcccatgac aagccgaaac tgacgcgctg gtcggataat gtgcgcattc 1500

tcgaagggct ggcgcaaaac ggcatcatgg aggagcagga agcgcaggca ttgacgctgg 1560

cgtacaccac attgcgtgat gagctgcacc acctggcgct gcaagagttg ccgggacatg 1620

tggcgctctc ctgttttgtc gccgagcgtg cgcttattaa aaccagctgg gacaagtggc 1680

tggtggaacc gtgcgccccg gcgtaagtgt ggtatcatcg cgcgcaaatt ttgtatctct 1740

caggagacag gaatgaaagt gacgctgcca gagtttaagc aagccggtgt aatggtggtg 1800

ggtgatgtga tgctggatcg ttactggtat ggcccaacca gccgtatctc tcgggaagcg 1860

ccagtcccgg ttgttaaagt cgataccatt gaagagcgtc ctggcggcgc ggcaaacgtg 1920

gcgatgaata tcgcctcact gggcgccacg gcgcgtctgg ttggcctgac tggcattgac 1980

gatgcggcgc gcgcgctgag caaagcgctg gccgatgtta acgttaaatg tgacttcgtt 2040

tctgttccga cgcatcccac catcactaag ctgcgcgtgc tgtcgcgtaa ccagcagctg 2100

attcgcctgg actttgaaga gggttttgaa ggagtcgatc cgcaaccgat gcatgaacgc 2160

atcagccagg cgcttggtaa tattggcgcg ctggtgctgt cggatt 2206

<210> 251

<211> 199

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-amtB

<400> 251

tttcgctgaa ggtgtgacca tgggccatca ggtgctggtg cagctggaaa gtgttgccat 60

cactatcgtg tggtctggcg tggtggcctt tattggttac aaactggcgg acatgacggt 120

aggcctgcgc gtaccggaag aacaagaacg tgaagggctg gatgtaaaca gccacggcga 180

aaacgcctat aacgcctga 199

<210> 252

<211> 1199

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-amtB with a 500 bp flanking region.

<400> 252

tttcctttct gactctgcc gtccgggcgc actaacggcc tgaaatactc cctcttttca 60

ttcctggcac aacgattgca atgtctgttg cgtgttagct gcggccatta tcgaattcga 120

ctggaggggg atctatgaag ctggttaccg tggtgattaa gccattcaaa cttgaagacg 180

tgcgtgaagc gctttcttct attggtattc aagggttgac cgtaactgaa gtgaaaggct 240

ttggccgtca gaagggtcac gctgagctgt accgcggtgc ggaatatagc gttaatttcc 300

tgccgaaagt gaaaattgat gtggcgatcg ctgacgatca actcgatgaa gtaatcgatg 360

tgatcagcaa agcggcctac accggaaaaa ttggcgacgg caaaattttc gttgctgagc 420

tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga agcggcactg taatacaaga 480

cacacagtga tggggatcgg tttcgctgaa ggtgtgacca tgggccatca ggtgctggtg 540

cagctggaaa gtgttgccat cactatcgtg tggtctggcg tggtggcctt tattggttac 600

aaactggcgg acatgacggt aggcctgcgc gtaccggaag aacaagaacg tgaagggctg 660

gatgtaaaca gccacggcga aaacgcctat aacgcctgat tgcgttgagt tatctcctga 720

gcataaaaaa gcctccattc ggaggctttt cttttttttaa gtttaaagcg cggttagttg 780

cgattgcgca tgacgccttc ctgcacgctg gacgcgacca gcacaccctc ttgcgtatag 840

aactcgccgc gcacaaaacc gcgagcgctg gaggctgacg tgctttccac actgtagagc 900

agccattcgt tcatattaaa cgggcgatgg aaccacatgg agtggtcaat ggtggcaacc 960

tgcataccgc gctcaaggaa gcccacgccg tgcggctgaa gtgcaaccgg caggaagtta 1020

aagtctgagg catatccaag cagatattga tgtacgcgaa aatcgtccgg caccgtgccg 1080

1140

aactcaaccg ggcggatctc cagtggttta tcactaagaa acttctcttt ggcctgcgg 1199

<210> 253

<211> 1563

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-1

<400> 253

atgtttaacg atctgattgg cgatgatgaa acggattcgc cggaagatgc gctttctgag 60

agctggcgcg aattgtggca ggatgcgttg caggaggagg attccacgcc cgtgctggcg 120

catctctcag aggacgatcg ccgccgcgtg gtggcgctga ttgccgattt tcgcaaagag 180

ttggataaac gcaccattgg cccgcgaggg cggcaggtac tcgatcactt aatgccgcat 240

ctgctcagcg atgtatgctc gcgcgacgat gcgccagtac cgctgtcacg cctgacgccg 300

ctgctcaccg gaattattac ccgcaccact taccttgagc tgctaagtga atttcccggc 360

gcactgaaac acctcatttc cctgtgtgcc gcgtcgccga tggttgccag tcagctggcg 420

cgctacccga tcctgcttga tgaattgctc gacccgaata cgctctatca accgacggcg 480

atgaatgcct atcgcgatga gctgcgccaa tacctgctgc gcgtgccgga agatgatgaa 540

gagcaacagc ttgaggcgct gcggcagttt aagcaggcgc agttgctgcg cgtggcggcg 600

gcggatattg ccggtacgtt gccagtaatg aaagtgagcg atcacttaac ctggctggcg 660

gaagcgatta ttgatgcggt ggtgcagcaa gcctgggggc agatggtggc gcgttatggc 720

cagccaacgc atctgcacga tcgcgaaggg cgcggttttg cggtggtcgg ttatggcaag 780

ctgggcggct gggagctggg ttacagctcc gatctggatc tggtattcct gcacgactgc 840

ccgatggatg tgatgaccga tggcgagcgt gaaatcgatg gtcgccagtt ctatttgcgt 900

ctcgcgcagc gcgtgatgca cctgtttagc acgcgcacgt cgtccggcat cctttatgaa 960

gttgatgcgc gtctgcgtcc atctggcgct gcggggatgc tggtcactac tacggaatcg 1020

ttcgccgatt accagcaaaa cgaagcctgg acgtgggaac atcaggcgct ggcccgtgcg 1080

cgcgtggtgt acggcgatcc gcaactgacc gccgaatttg acgccattcg ccgcgatatt 1140

ctgatgacgc ctcgcgacgg cgcaacgctg caaaccgacg tgcgagaaat gcgcgagaaa 1200

atgcgtgccc atcttggcaa caagcataaa gaccgcttcg atctgaaagc cgatgaaggc 1260

ggtatcaccg acatcgagtt tatcgcccaa tatctggtgc tgcgctttgc ccatgacaag 1320

ccgaaactga cgcgctggtc ggataatgtg cgcattctcg aagggctggc gcaaaacggc 1380

atcatggagg agcaggaagc gcaggcattg acgctggcgt acaccacatt gcgtgatgag 1440

ctgcaccacc tggcgctgca agagttgccg ggacatgtgg cgctctcctg ttttgtcgcc 1500

1560 gagcgtgcgc ttattaaaac cagctgggac

taa 1563

<210> 254

<211> 2563

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-1 with a 500 bp flanking region.

<400> 254

gcgcaaagcg agtgctcact tacgtgatct gttgacacaa tctgaagcga ccataacttc 60

tgccgtttca gcgaatacgg cggtgtggag cgcacaatca gccctggcga agctggtgct 120

caccgagtgg ctagtgacgc agggctggcg aaccttcctt gatgaaaaag cgcaggccaa 180

attcgccgac tcctttaaac gctttgctga catccatctg tcacgcagcg ccgccgagct 240

gaaaaaagcc tttgcccaac cgctgggcga cagctatcgc gaccagttgc cgcgcctggc 300

gcgtgatatc gactgcgcgt tactgctggc cgggcattac gatcgcgcgc gcgccgtgga 360

atggctggaa aactggcagg ggcttcagca cgccattgaa acgcgccaga gagtcgaaat 420

cgaacatttc cgtaataccg cgattaccca ggagccgttc tggttgcaca gcggaaaacg 480

ttaacgaaag gatatttcgc atgtttaacg atctgattgg cgatgatgaa acggattcgc 540

cggaagatgc gctttctgag agctggcgcg aattgtggca ggatgcgttg caggaggagg 600

attccacgcc cgtgctggcg catctctcag aggacgatcg ccgccgcgtg gtggcgctga 660

ttgccgattt tcgcaaagag ttggataaac gcaccattgg cccgcgaggg cggcaggtac 720

tcgatcactt aatgccgcat ctgctcagcg atgtatgctc gcgcgacgat gcgccagtac 780

cgctgtcacg cctgacgccg ctgctcaccg gaattattac ccgcaccact taccttgagc 840

tgctaagtga atttcccggc gcactgaaac acctcatttc cctgtgtgcc gcgtcgccga 900

tggttgccag tcagctggcg cgctacccga tcctgcttga tgaattgctc gacccgaata 960

cgctctatca accgacggcg atgaatgcct atcgcgatga gctgcgccaa tacctgctgc 1020

gcgtgccgga agatgatgaa gagcaacagc ttgaggcgct gcggcagttt aagcaggcgc 1080

agttgctgcg cgtggcggcg gcggatattg ccggtacgtt gccagtaatg aaagtgagcg 1140

atcacttaac ctggctggcg gaagcgatta ttgatgcggt ggtgcagcaa gcctgggggc 1200

agatggtggc gcgttatggc cagccaacgc atctgcacga tcgcgaaggg cgcggttttg 1260

cggtggtcgg ttatggcaag ctgggcggct gggagctggg ttacagctcc gatctggatc 1320

tggtattcct gcacgactgc ccgatggatg tgatgaccga tggcgagcgt gaaatcgatg 1380

gtcgccagtt ctatttgcgt ctcgcgcagc gcgtgatgca cctgtttagc acgcgcacgt 1440

cgtccggcat cctttatgaa gttgatgcgc gtctgcgtcc atctggcgct gcggggatgc 1500

tggtcactac tacggaatcg ttcgccgatt accagcaaaa cgaagcctgg acgtgggaac 1560

atcaggcgct ggcccgtgcg cgcgtggtgt acggcgatcc gcaactgacc gccgaatttg 1620

acgccattcg ccgcgatatt ctgatgacgc ctcgcgacgg cgcaacgctg caaaccgacg 1680

tgcgagaaat gcgcgagaaa atgcgtgccc atcttggcaa caagcataaa gaccgcttcg 1740

atctgaaagc cgatgaaggc ggtatcaccg acatcgagtt tatcgcccaa tatctggtgc 1800

tgcgctttgc ccatgacaag ccgaaactga cgcgctggtc ggataatgtg cgcattctcg 1860

aagggctggc gcaaaacggc atcatggagg agcaggaagc gcaggcattg acgctggcgt 1920

acaccacatt gcgtgatgag ctgcaccacc tggcgctgca agagttgccg ggacatgtgg 1980

cgctctcctg ttttgtcgcc gagcgtgcgc ttattaaaac cagctgggac aagtggctgg 2040

tggaaccgtg cgccccggcg taagtgtggt atcatcgcgc gcaaattttg tatctctcag 2100

gagacaggaa tgaaagtgac gctgccagag tttaagcaag ccggtgtaat ggtggtgggt 2160

gatgtgatgc tggatcgtta ctggtatggc ccaaccagcc gtatctctcc ggaagcgcca 2220

gtcccggttg ttaaagtcga taccattgaa gagcgtcctg gcggcgcggc aaacgtggcg 2280

atgaatatcg cctcactggg cgccacggcg cgtctggttg gcctgactgg cattgacgat 2340

gcggcgcgcg cgctgagcaa agcgctggcc gatgttaacg ttaaatgtga cttcgtttct 2400

gttccgacgc atcccaccat cactaagctg cgcgtgctgt cgcgtaacca gcagctgatt 2460

cgcctggact ttgaagaggg ttttgaagga gtcgatccgc aaccgatgca tgaacgcatc 2520

agccaggcgc ttggtaatat tggcgcgctg gtgctgtcgg att 2563

<210> 255

<211> 461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1

<400> 255

atgaccctga atatgatgat ggatgccggc cgtcctgtaa taataaccgg acaattcgga 60

ctgattaaaa aagcgccctt gtggcgcttt ttttatattc ccgcctccat ttaaaataaa 120

aaatccaatc ggatttcact atttaaactg gccattatct aagatgaatc cgatggaagc 180

240

cgatcaaatt taagtggatt cccatcaaaa aaatattctc aacctaaaaa agtttgtgta 300

atacttgtaa cgctacatgg agattaactc aatctagagg gtattaataa tgaatcgtac 360

taaactggta ctgggcgcaa ctcacttcac accccgaagg gggaagttgc ctgaccctac 420

gattcccgct atttcattca ctgaccggag gttcaaaatg a 461

<210> 256

<211> 1461

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm1 with a 500 bp flanking region.

<400> 256

accggatacg agagaaaagt gtctacatcg gttcggttga tattgaccgg cgcatccgcc 60

agcccgccca gtttctggtg gatctgtttg gcgattttgc gggtcttgcc ggtgtcggtg 120

ccgaaaaaaa taccaatatt tgccataaca cacgctcctg ttgaaaaaga gatcccgccg 180

ggaaatgcgg tgaacgtgtc tgatattgcg aagagtgtgc cagttttggt cgcgggcaaa 240

acctgcacca gtttggttat taatgcacca gtctggcgct ttttttcgcc gagtttctcc 300

tcgctaatgc ccgccaggcg cggctttggc gctgatagcg cgctgaatac cgatctggat 360

caaggttttg tcgggttatc agccaaaagg tgcactcttt gcatggttat acgtgcctga 420

catgttgtcc gggcgacaaa cggcctggtg gcacaaattg tcagaactac gacacgacta 480

actgaccgca ggagtgtgcg atgaccctga atatgatgat ggatgccggc cgtcctgtaa 540

taataaccgg acaattcggga ctgattaaaa aagcgccctt gtggcgcttt ttttatattc 600

ccgcctccat ttaaaataaa aaatccaatc ggatttcact atttaaactg gccattatct 660

aagatgaatc cgatggaagc tcgctgtttt aacacgcgtt ttttaacctt ttattgaaag 720

tcggtgcttc tttgagcgaa cgatcaaatt taagtggatt cccatcaaaa aaatattctc 780

aacctaaaaa agtttgtgta atacttgtaa cgctacatgg agattaactc aatctagagg 840

gtattaataa tgaatcgtac taaactggta ctgggcgcaa ctcacttcac accccgaagg 900

gggaagttgc ctgaccctac gattcccgct atttcattca ctgaccggag gttcaaaatg 960

acccagcgaa ccgagtcggg taataccgtc tggcgcttcg atttgtccca gcagttcact 1020

gcgatgcagc gcataagcgt ggtactcagc cgggcgaccg aggtcgatca gacgctccag 1080

caagtgctgt gcgtattgca caatgacgcc ttttgcagc acggcatgat ctgtctgtac 1140

gacagccagc aggcgatttt gaatattgaa gcgttgcagg aagccgatca gcagttaatc 1200

cccggcagct cgcaaatccg ctatcgtccg ggcgaagggc tggtcgggac ggtgctttcg 1260

cagggccaat cattagtgct ggcgcgcgtt gctgacgatc agcgctttct tgaccggctc 1320

gggttgtatg attacaacct gccgtttatc gccgtgccgc tgatagggcc agatgcgcag 1380

actttcggtg tgctgacggc acaacccatg gcgcgttacg aagagcgatt acccgcctgc 1440

acccgctttc tggaaacggt c 1461

<210> 257

<211> 199

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-amtB

<400> 257

tttcgctgaa ggtgtgacca tgggccatca ggtgctggtg cagctggaaa gtgttgccat 60

cactatcgtg tggtctggcg tggtggcctt tattggttac aaactggcgg acatgacggt 120

aggcctgcgc gtaccggaag aacaagaacg tgaagggctg gatgtaaaca gccacggcga 180

aaacgcctat aacgcctga 199

<210> 258

<211> 1199

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-amtB with a 500 bp flanking region.

<400> 258

tttcctttct gactctgcc gtccgggcgc actaacggcc tgaaatactc cctcttttca 60

ttcctggcac aacgattgca atgtctgttg cgtgttagct gcggccatta tcgaattcga 120

ctggaggggg atctatgaag ctggttaccg tggtgattaa gccattcaaa cttgaagacg 180

tgcgtgaagc gctttcttct attggtattc aagggttgac cgtaactgaa gtgaaaggct 240

ttggccgtca gaagggtcac gctgagctgt accgcggtgc ggaatatagc gttaatttcc 300

tgccgaaagt gaaaattgat gtggcgatcg ctgacgatca actcgatgaa gtaatcgatg 360

tgatcagcaa agcggcctac accggaaaaa ttggcgacgg caaaattttc gttgctgagc 420

tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga agcggcactg taatacaaga 480

cacacagtga tggggatcgg tttcgctgaa ggtgtgacca tgggccatca ggtgctggtg 540

cagctggaaa gtgttgccat cactatcgtg tggtctggcg tggtggcctt tattggttac 600

aaactggcgg acatgacggt aggcctgcgc gtaccggaag aacaagaacg tgaagggctg 660

gatgtaaaca gccacggcga aaacgcctat aacgcctgat tgcgttgagt tatctcctga 720

gcataaaaaa gcctccattc ggaggctttt cttttttttaa gtttaaagcg cggttagttg 780

cgattgcgca tgacgccttc ctgcacgctg gacgcgacca gcacaccctc ttgcgtatag 840

aactcgccgc gcacaaaacc gcgagcgctg gaggctgacg tgctttccac actgtagagc 900

agccattcgt tcatattaaa cgggcgatgg aaccacatgg agtggtcaat ggtggcaacc 960

tgcataccgc gctcaaggaa gcccacgccg tgcggctgaa gtgcaaccgg caggaagtta 1020

aagtctgagg catatccaag cagatattga tgtacgcgaa aatcgtccgg caccgtgccg 1080

1140

aactcaaccg ggcggatctc cagtggttta tcactaagaa acttctcttt ggcctgcgg 1199

<210> 259

<211> 607

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::PinfC

<400> 259

atgaccttta atatgatgcc tggggtcact ggagcgcttt atcggcatcc tgaccgaaga 60

atttgccggt ttcttcccga cctggctggc ccctgttcag gttgtggtga tgaatatcac 120

tgattctcaa gctgaatatg tcaacgaatt gacccgtaaa ttgcaaaatg cgggcattcg 180

tgtaaaagcg gacttgagaa acgagaagat tggctttaaa atccgcgagc acactttacg 240

tcgtgtccct tatatgttgg tctgtggtga taaagaggtg gaagcaggca aagtggccgt 300

tcgcacccgc cgcggtaaag acctgggcag cctggacgta agtgaagtga ttgagaagct 360

gcaacaagag attcgcagcc gcagtcttca acaactggag gaataaggta ttaaaggcgg 420

aaaacgagtt caaacggcac gtccgaatcg tatcaatggc gagattcgcg ccggaagt 480

tcgcttaact ggtctggaag gtgagcagct gggtattgca atagaactaa ctacccgccc 540

tgaaggcggt acctgcctga ccctgcgatt cccgttattt cattcactga ccggaggccc 600

acgatga 607

<210> 260

<211> 1607

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::PinfC with a 500 bp flanking region.

<400> 260

ggtacgacaa aaacgtctcc agcgacgtgc ggttaatatt gactggcgca tccgccacat 60

cccccagttt ttgctggatc agtttggcga ttttgcgggt ttttcccgtg tcactgccaa 120

aaaaaatacc aatgttagcc atgtcgcgct cctgttgaga aagaataagg ccgcctgcaa 180

acggcggata tcccttctcc tgttgcgaag gctgtgccag gtttttttaa ggccttctgt 240

gcactgaaat gggtgaaaaa atgactcttt tttgtgcagg caccgtcctc tctccgctat 300

ccagacctgc tttgaaggcc tctgaggggcc aaatcagggc caaaacacga atcaggatca 360

atgtttcggc gcgttacctg ttcgaaaggt gcactctttg catggttaat cacacccaat 420

cagggctgcg gatgtcgggc gtttcacaac acaaaatgtt gtaaatgcga cacagccggg 480

cctgaaacca ggagcgtgtg atgaccttta atatgatgcc tggggtcact ggagcgcttt 540

atcggcatcc tgaccgaaga atttgccggt ttcttcccga cctggctggc ccctgttcag 600

gttgtggtga tgaatatcac tgattctcaa gctgaatatg tcaacgaatt gacccgtaaa 660

ttgcaaaatg cgggcattcg tgtaaaagcg gacttgagaa acgagaagat tggctttaaa 720

atccgcgagc acactttacg tcgtgtccct tatatgttgg tctgtggtga taaagaggtg 780

gaagcaggca aagtggccgt tcgcacccgc cgcggtaaag acctgggcag cctggacgta 840

agtgaagtga ttgagaagct gcaacaagag attcgcagcc gcagtcttca acaactggag 900

gaataaggta ttaaaggcgg aaaacgagtt caaacggcac gtccgaatcg tatcaatggc 960

gagattcgcg cccaggaagt tcgcttaact ggtctggaag gtgagcagct gggtattgca 1020

atagaactaa ctacccgccc tgaaggcggt acctgcctga ccctgcgatt cccgttattt 1080

cattcactga cgggaggccc acgatgaccc agcgacccga gtcgggcacc accgtctggc 1140

gttttgatct ctcacagcaa tttaccgcca tgcagcgcat cagcgtggtg ttgagtcgcg 1200

1260

tgcaacacgg catgctgtgt ctgtatgaca accagcagga aattctgagt attgaagcct 1320

tgcaggaggc agaccaacat ctgatccccg gcagctcgca aattcgctat cgccctggcg 1380

aagggctggt aggagccgta ctgtcccagg gacaatctct tgtgctgccg cgtgtcgccg 1440

acgatcaacg ctttctcgac aggcttggca tctatgatta caacctgccg tttatcgccg 1500

tccccttaat ggggccaggc gcgcagacga ttggcgtgct cgccgcgcag ccgatggcgc 1560

gtctggagga gcggcttcct tcctgtacgc gctttctgga aaccgtc 1607

<210> 261

<211> 1536

<212> DNA

<213> Kosakonia sacchari

<220>

<223> 16S-1

<400> 261

ttgaagagtt tgatcatggc tcagattgaa cgctggcggc aggcctaaca catgcaagtc 60

gaacggtagc acagagagct tgctctcggg tgacgagtgg cggacgggtg agtaatgtct 120

gggaaactgc ctgatggagg gggataacta ctggaaacgg tagctaatac cgcataacgt 180

cgcaagacca aagaggggga ccttcgggcc tcttgccatc agatgtgccc agatgggatt 240

agctagtagg tggggtaacg gctcacctag gcgacgatcc ctagctggtc tgagaggatg 300

accagccaca ctggaactga gacacggtcc agactcctac gggaggcagc agtggggaat 360

attgcacaat gggcgcaagc ctgatgcagc catgccgcgt gtgtgaagaa ggccttcggg 420

ttgtaaagca ctttcagcgg ggaggaaggg agtaaggtta ataaccttat tcattgacgt 480

tacccgcaga agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc 540

aagcgttaat cggaattact gggcgtaaag cgcacgcagg cggtctgtca agtcggatgt 600

gaaatccccg ggctcaacct gggaactgca tccgaaactg gcaggcttga gtctcgtaga 660

gggaggtaga attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg 720

cgaaggcggc ctcctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag 780

gattagatac cctggtagtc cacgccgtaa acgatgtcta tttggaggtt gtgcccttga 840

ggcgtggctt ccggagctaa cgcgttaaat agaccgcctg gggagtacgg ccgcaaggtt 900

aaaactcaaa tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat 960

gcaacgcgaa gaaccttacc tggtcttgac atccacagaa ctttccagag atggattggt 1020

gccttcggga actgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt 1080

1140

actcaaagga gactgccagt gataaactgg aggaaggtgg ggatgacgtc aagtcatcat 1200

ggcccttacg accagggcta cacacgtgct acaatggcgc atacaaagag aagcgacctc 1260

gcgagagtaa gcggacctca taaagtgcgt cgtagtccgg attggagtct gcaactcgac 1320

1380

ggccttgtac acaccgcccg tcacaccatg ggagtgggtt gcaaaagaag taggtagctt 1440

aaccttcggg agggcgctta ccactttgtg attcatgact ggggtgaagt cgtaacaagg 1500

taaccgtagg ggaacctgcg gttggatcac ctcctt 1536

<210> 262

<211> 1537

<212> DNA

<213> Kosakonia sacchari

<220>

<223> 16S-2

<220>

<221> modified base

<222> (450)..(450)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (452)..(452)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (455)..(455)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (473)..(473)

<223> a, c, t, g, unknown or other

<400> 262

ttgaagagtt tgatcatggc tcagattgaa cgctggcggc aggcctaaca catgcaagtc 60

gaacggtagc acagagagct tgctctcggg tgacgagtgg cggacgggtg agtaatgtct 120

gggaaactgc ctgatggagg gggataacta ctggaaacgg tagctaatac cgcataacgt 180

cgcaagacca aagaggggga ccttcgggcc tcttgccatc agatgtgccc agatgggatt 240

agctagtagg tggggtaacg gctcacctag gcgacgatcc ctagctggtc tgagaggatg 300

accagccaca ctggaactga gacacggtcc agactcctac gggaggcagc agtggggaat 360

attgcacaat gggcgcaagc ctgatgcagc catgccgcgt gtgtgaagaa ggccttcggg 420

ttgtaaagca ctttcagcgg ggaggaaggn antanggtta ataacctgtg ttnattgacg 480

ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat acggagggtg 540

caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtctgtc aagtcggatg 600

tgaaatcccc gggctcaacc tgggaactgc atccgaaact ggcaggcttg agtctcgtag 660

agggaggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg aataccggtg 720

gcgaaggcgg ccctcctggac gaagactgac gctcaggtgc gaaagcgtgg ggagcaaaca 780

ggattagata ccctggtagt ccacgccgta aacgatgtct atttggaggt tgtgcccttg 840

aggcgtggct tccggagcta acgcgttaaa tagaccgcct ggggagtacg gccgcaaggt 900

taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga 960

tgcaacgcga agaaccttac ctggtcttga catccacaga acttagcaga gatgctttgg 1020

tgccttcggg aactgtgaga caggtgctgc atggctgtcg tcagctcgtg ttgtgaaatg 1080

ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg ttaggccggg 1140

aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt caagtcatca 1200

tggcccttac gaccaggggct acacacgtgc tacaatggcg catacaaaga gaagcgacct 1260

1320 cgcgagagta agcggacctc ataaagtgcg tcgtagtccg

ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga atacgttccc 1380

gggccttgta cacaccgcc gtcacaccat gggagtgggt tgcaaaagaa gtaggtagct 1440

taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag tcgtaacaag 1500

gtaaccgtag gggaacctgc ggttggatca ccttcctt 1537

<210> 263

<211> 882

<212> DNA

<213> Kosakonia sacchari

<220>

<223> nifH

<400> 263

atgaccatgc gtcaatgcgc catttacggc aaaggtggga tcggcaaatc gaccaccaca 60

cagaacctgg tcgccgcgct ggcggagatg ggtaaaaaag tcatgattgt cggctgtgac 120

ccgaaagccg attccacgcg tttgatcctg catgcgaaag cgcagaacac cattatggag 180

atggctgctg aagtcggctc cgtggaagac ctggagttag aagacgtgct gcaaatcggt 240

tacggcggcg tgcgctgcgc agagtccggc ggcccggagc caggcgtggg ctgtgccggt 300

cgcggggtga tcaccgcgat taacttcctc gaagaagaag gcgcttacgt gccggatctc 360

gtggttgcg gtggtttcgc catgccgatt 420

cgtgaaaaca aagcgcagga gatctacatc gtttgctctg gcgaaatgat ggcgatgtac 480

gccgccaaca acatctccaa aggcatcgtg aaatacgcca aatccggtaa agtgcgcctc 540

ggcgggctga tttgtaactc gcgccagacc gaccgtgaag atgaactgat cattgcgctg 600

gcagaaaaac tcggcacgca gatgatccac tttgttcccc gcgacaacat tgtgcagcgt 660

gcggaaatcc gccgtatgac ggttatcgaa tatgacccga cctgcaatca ggcgaacgaa 720

tatcgcagcc ttgccagcaa aatcgtcaac aacaccaaaa tggtggtgcc caccccctgc 780

accatggatg aactggaaga actgctgatg gagttcggca ttatggatgt ggaagacacc 840

agcatcattg gtaaaaccgc cgccgaagaa aacgccgtct ga 882

<210> 264

<211> 1449

<212> DNA

<213> Kosakonia sacchari

<220>

<223> nifD2

<400> 264

atgagcaatg caacaggcga acgcaacctg gagataatcg agcaggtgct cgaggttttc 60

ccggagaaga cgcgcaaaga acgcagaaaa cacatgatgg tgacggaccc ggagcaggaa 120

agcgtcggta agtgcatcat ctctaaccgc aaatcgcagc caggcgtgat gaccgtgcgc 180

ggctgctcgt atgccggttc gaaaggggtg gtatttgggc caatcaagga tatggcgcat 240

atctcgcatg gcccaatcgg ctgcggccaa tactcccgcg ccgggcggcg gaactactac 300

accggcgtca gcggcgtgga cagcttcggc acgctcaact tcacctccga ttttcaggag 360

cgcgacatcg tgtttggcgg cgataaaaag ctcgccaaac tgattgaaga gctggaagag 420

ctgttcccgc tgaccaaagg catttcgatt cagtcggaat gcccggtcgg cctgattggc 480

gatgacattg aggccgtcgc gaacgccagc cgcaaagcca tcaacaaacc ggttattccg 540

gtgcgttgcg aaggctttcg cggcgtgtcg caatccctcg gtcaccatat tgccaacgat 600

gtgatccgcg actgggtgct ggataaccgc gaaggcaaac cgttcgaatc caccccttac 660

gatgtggcga tcatcggcga ttacaacatc ggcggcgatg cctgggcttc gcgcattttg 720

ctcgaagaga tgggcttgcg ggtggtggca cagtggtctg gcgacggtac gctggtggag 780

atggaaaaca cgccgttcgt caaactgaac ctggtgcatt gttaccgctc aatgaactac 840

atctcgcgcc atatggagga gaagcacggt attccgtgga tggaatacaa cttctttggt 900

ccgacgaaaa tcgcggaatc gctgcgcaaa atcgccgacc agtttgacga caccattcgc 960

gccaacgccg aagcggtgat cgccagatac caggcgcaaa acgacgccat tatcgccaaa 1020

tatcgcccgc gtctggaggg gcgcaaagtg ctgctttata tgggcgggct gcgtccgcgc 1080

catgtgattg gcgcctatga agacctggga atggagatca tcgctgccgg ttatgagttc 1140

ggtcataacg atgattacga ccgcaccttg ccggatctga aagagggcac gctgctgttt 1200

gatgatgcca gcagttatga gctggaggcg ttcgtcaacg cgctgaaacc ggatctcatc 1260

ggttccggca tcaaagagaa gtacatcttt cagaaaatgg gcgtgccgtt tcgccagatg 1320

cactcctggg attactccgg cccgtaccac ggctatgacg gcttcgccat cttcgcccgc 1380

gatatggata tgacgctcaa caaccccgcg tggggccagt tgaccgcgcc gtggctgaaa 1440

tccgcctga 1449

<210> 265

<211> 1563

<212> DNA

<213> Kosakonia sacchari

<220>

<223> nifK2

<400> 265

atgagccaga ctgctgagaa aatacagaat tgccatcccc tgtttgaaca ggatgcttac 60

cagacgctgt ttgccggtaa acgggcactc gaagaggcgc actcgccgga gcgggtgcag 120

180

ctgactatcg acccggcaaa agcctgccag ccgctgggcg cggtgctctg ttcgctgggg 240

tttgccaata ccctaccgta tgtgcacggt tcacaggggtt gcgtggccta tttccgcacg 300

360

gcggcggtgt tcggcgggaa taacaacctc aacaccggct tacaaaacgc cagcgcgctg 420

tataaaccgg agattatcgc cgtctctacc acctgtatgg cggaagtgat cggtgatgat 480

ttgcaggcct ttatcgccaa cgccaaaaaa gatggttttc tcgatgccgc catccccgtg 540

ccctacgcgc acacccccag ttttatcggc agccatatca ccggctggga taacatgttt 600

gaaggttttg cccggacctt tacggcagac catgaagctc agcccggcaa actttcacgc 660

atcaacctgg tgaccgggtt tgaaacctat ctcggcaatt tccgcgtgct gaaacgcatg 720

atggaacaaa tggaggtgcc ggcgagtgtg ctctccgatc cgtcggaagt gctggatact 780

cccgccaacg ggcattacca gatgtacgcg ggcgggacga cgcagcaaga gatgcgcgag 840

gcgccggatg ctatcgacac cctgttgctg cagccctggc aactggtgaa aagcaaaaaa 900

gtggtgcagg agatgtggaa tcagcccgcc accgaggttt ctgttcccgt tgggctggca 960

ggaacagacg aactgttgat ggcgattagc cagttaaccg gcaaggccat tcccgattca 1020

ctggcgctgg agcgcgggcg gctggtcgat atgatgctcg attcccacac ctggttgcac 1080

ggtaaaaaat tcggcctgtt tggcgatccg gattttgtca tgggattgac ccgtttcctg 1140

ctggagctgg gctgcgaacc gaccgttatc ctctgccaca acggtaacaa acgctggcag 1200

1260

aactgcgatt tgtggcattt ccgctcgctg atgtttaccc gccagccgga ttttatgatt 1320

ggcaactcgt acggcaagtt cattcagcgc gacaccttag ccaaaggcga gcagtttgaa 1380

gttccgctga tccgcctcgg ttttcccctg ttcgaccgcc accatctgca ccgccagacc 1440

acctggggct acgagggcgc catgagcatt ctcactaccc ttgtgaatgc ggtactggag 1500

1560

taa 1563

<210> 266

<211> 1488

<212> DNA

<213> Kosakonia sacchari

<220>

<223> nifL

<400> 266

atgaccctga atatgatgat ggatgccggc gcgcccgagg caatcgccgg tgcgctttcg 60

120

actgatgccg acgcacgcat tgtctatgcc aacccggctt tctgccgcca gaccggctat 180

gaactagaag cgttgttgca gcaaaatccc cgcctgcttg caagtcgcca aacccccgg 240

gaaatctatc aggatatgtg gcacaccttg ttacaacgcc gaccgtggcg cgggcaattg 300

attaaccgcc accgcgacgg cagcctgtat ctggtcgaga tcgatatcac cccggtgatt 360

aacccgtttg gcgaactgga acactacctg gcaatgcagc gcgatatcag cgccagttat 420

gcgctggagc agcggttgcg caatcacatg acgctgaccg aagcggtgct gaataacatt 480

ccggcggcgg tggttgtagt ggatgaacgc gatcatgtgg ttatggataa ccttgcctac 540

aaaacgttct gtgccgactg cggcggaaaa gagctcctga gcgaactcaa tttttcagcc 600

660

tggttgtcgg tgacctgctg ggcgctgccg ggcgtcagcg aagaagccag tcgctacttt 720

attgataaca ggctgacgcg cacgctggtg gtgatcaccg acgacaccca acaacgccag 780

cagcaggaac agggccgact tgaccgcctt aaacagcaga tgaccaacgg caaactactg 840

gcagcgatcc gcgaagcgct tgacgccgcg ctgatccagc ttaactgccc catcaatatg 900

ctggcggcgg cgcgacgttt aaacggcagt gataacaaca atgtggcgct cgacgccgcg 960

tggcgcgaag gtgaagaggc gatggcgcgg ctgaaacgtt gccgcccgtc gctggaactg 1020

gaaagtgcgg ccgtctggcc gctgcaaccc ttttttgacg atctgcgcgc gctttatcac 1080

acccgctacg agcaggggaa aaatttgcag gtcacgctgg attcccatca tctggtggga 1140

tttggtcagc gtacgcaact gttagcctgc ctgagtctgt ggctcgatcg cacgctggat 1200

1260

ggctggctct ctttgtatat cactgacaat gtgccgctga tcccgctgcg cccacacccac 1320

1380 tcgccggatg cgcttaacgc tccgggaaaa ggcatggagc

gtggcacacc accacggcgc aatagaactc acttcacacc ccgaaggggg aagttgcctg 1440

accctacgat tcccgctatt tcattcactg accggaggtt caaaatga 1488

<210> 267

<211> 1575

<212> DNA

<213> Kosakonia sacchari

<220>

<223> nifA

<400> 267

atgacccagc gaaccgagtc gggtaatacc gtctggcgct tcgatttgtc ccagcagttc 60

actgcgatgc agcgcataag cgtggtactc agccgggcga ccgaggtcga tcagacgctc 120

cagcaagtgc tgtgcgtatt gcacaatgac gccttttttgc agcacggcat gatctgtctg 180

tacgacagcc agcaggcgat tttgaatatt gaagcgttgc aggaagccga tcagcagtta 240

atccccggca gctcgcaaat ccgctatcgt ccgggcgaag ggctggtcgg gacggtgctt 300

tcgcagggcc aatcattagt gctggcgcgc gttgctgacg atcagcgctt tcttgaccgg 360

ctcgggttgt atgattacaa ccgccgttt atcgccgtgc cgctgatagg gccagatgcg 420

cagactttcg gtgtgctgac ggcacaaccc atggcgcgtt acgaagagcg attacccgcc 480

tgcacccgct ttctggaaac ggtcgctaac ctggtcgcgc aaaccgtgcg tttgatggca 540

ccaccggcag tgcgcccttc cccgcgcgcc gccataacac aggccgccag cccgaaatcc 600

tgcacggcct cacgcgcatt tggttttgaa aatatggtcg gtaacagtcc ggcgatgcgc 660

cagaccatgg agattatccg tcaggtttcg cgctgggaca ccaccgttct ggtacgcggc 720

gagagtggca ccggcaagga gctgattgcc aacgccatcc accaccattc gccgcgtgcc 780

ggtgcgccat ttgtgaaatt caactgtgcg gcgctgccgg acacactgct ggaaagcgaa 840

ttgttcggtc acgagaaagg ggcatttacc ggcgcggtac gccagcgtaa aggccgtttt 900

gagctggccg atggcggcac gctgtttctt gacgagatcg gcgagagtag cgcctcgttt 960

caggctaagc tgctgcgcat tttgcaggaa ggcgaaatgg aacgcgtcgg cggcgacgag 1020

acattgcaag tgaatgtgcg cattattgcc gcgacgaacc gcaatcttga agatgaagtc 1080

cggctggggc actttcgcga agatctctat tatcgcctga atgtgatgcc catcgccctg 1140

ccgccactac gcgaacgcca ggaggacatt gccgagctgg cgcactttct ggtgcgtaaa 1200

atcgcccata accagagccg tacgctgcgc attagcgagg gcgctatccg cctgctgatg 1260

agctacaact ggcccggtaa tgtgcgcgaa ctggaaaact gccttgagcg ctcagcggtg 1320

atgtcggaga acggtctgat cgatcgggat gtgattttgt ttaatcatcg cgaccagcca 1380

gccaaaccgc cagttatcag cgtctcgcat gatgataact ggctcgataa caaccttgac 1440

gagcgccagc ggctgattgc ggcgctggaa aaagcgggat gggtacaagc caaagccgcg 1500

cgcttgctgg ggatgacgcc gcgccaggtc gcctatcgta ttcagacgat ggatataacc 1560

ctgccaaggc tataa 1575

<210> 268

<211> 2850

<212> DNA

<213> Kosakonia sacchari

<220>

<223> glnE

<400> 268

atgccgcacc acgcaggatt gtcgcagcac tggcaaacgg tattttctcg tctgccggaa 60

tcgctcaccg cgcagccatt gagcgcgcag gcgcagtcag tgctcacttt tagtgatttt 120

gttcaggaca gcatcatcgc gcatcctgag tggctggcag agcttgaaag cgcgccgccg 180

cctgcgaacg aatggcaaca ctatgcgcaa tggctgcaag cggcgctgga tggcgtcacc 240

gatgaagcct cgctgatgcg cgcgctgcgg ctgtttcgcc gtcgcatcat ggtgcgcatc 300

gcctggagcc aggcgttaca gttggtggcg gaagaagata tcctgcaaca gcttagcgtg 360

ctggcggaaa ccctgatcgt cgccgcgcgc gactggcttt atgaggcctg ctgccgtgag 420

tggggaacgc cgagcaatcc acaaggcgtg gcgcagccga tgctggtact cggcatggggc 480

aaactgggtg gcggcgaact caatttctca tccgatatcg atttgatttt cgcctggccg 540

gaaaatggcg caacgcgcgg tggacgccgt gagctggata acgcgcaatt tttcactcgc 600

cttggtcaac ggctgattaa agtcctcgac cagccaacgc aggatggctt tgtctaccgc 660

gtcgatatgc gcttgcgccc gtttggcgac agcggcccgc tggtgctgag ctttgccgcg 720

ctggaagatt actaccagga gcaggggcgc gattgggaac gctacgcgat ggtgaaagcg 780

cgcattatgg gcgataacga cggcgaccat gcgcgggagt tgcgcgcaat gctgcgcccg 840

tttgttttcc gccgttatat cgacttcagc gtgattcagt ccctgcgtaa catgaaaggc 900

atgattgccc gcgaagtgcg tcgccgtggc ctgaaggaca acattaagct cggcgcgggc 960

gggatccgcg aaatagaatt tatcgtccag gttttccagc tgattcgcgg cggtcgcgag 1020

cctgcactgc aatcgcgttc actgttgccg acgcttgctg ccatagatca actgcatctg 1080

ctgccggatg gcgacgcaac ccggctgcgc gaggcgtatt tgtggctgcg acggctggag 1140

aacctgctgc aaagcatcaa tgacgaacag aacacagacgc tgccgggcga tgaactgaat 1200

cgcgcgcgcc tcgcctgggg aatgggcaaa gatagctggg aagcgctctg cgaaacgctg 1260

1320

1380

gaggaggatt ccacgcccgt gctggcgcat ctctcagagg acgatcgccg ccgcgtggtg 1440

gcgctgattg ccgattttcg caaagagttg gataaacgca ccattggccc gcgagggcgg 1500

caggtactcg atcacttaat gccgcatctg ctcagcgatg tatgctcgcg cgacgatgcg 1560

ccagtaccgc tgtcacgcct gacgccgctg ctcaccggaa ttattacccg caccacttac 1620

cttgagctgc taagtgaatt tcccggcgca ctgaaacacc tcatttccct gtgtgccgcg 1680

tcgccgatgg ttgccagtca gctggcgcgc tacccgatcc tgcttgatga attgctcgac 1740

ccgaatacgc tctatcaacc gacggcgatg aatgcctatc gcgatgagct gcgccaatac 1800

ctgctgcgcg tgccggaaga tgatgaagag caacagcttg aggcgctgcg gcagtttaag 1860

caggcgcagt tgctgcgcgt ggcggcggcg gatattgccg gtacgttgcc agtaatgaaa 1920

gtgagcgatc acttaacctg gctggcggaa gcgattattg atgcggtggt gcagcaagcc 1980

tgggggcaga tggtggcgcg ttatggccag ccaacgcatc tgcacgatcg cgaagggcgc 2040

ggttttgcgg tggtcggtta tggcaagctg ggcggctggg agctgggtta cagctccgat 2100

ctggatctgg tattcctgca cgactgcccg atggatgtga tgaccgatgg cgagcgtgaa 2160

atcgatggtc gccagttcta tttgcgtctc gcgcagcgcg tgatgcacct gtttagcacg 2220

cgcacgtcgt ccggcatcct ttatgaagtt gatgcgcgtc tgcgtccatc tggcgctgcg 2280

gggatgctgg tcactactac ggaatcgttc gccgattacc agcaaaacga agcctggacg 2340

tgggaacatc aggcgctggc ccgtgcgcgc gtggtgtacg gcgatccgca actgaccgcc 2400

gaatttgacg ccattcgccg cgatattctg atgacgcctc gcgacggcgc aacgctgcaa 2460

accgacgtgc gagaaatgcg cgagaaaatg cgtgcccatc ttggcaacaa gcataaagac 2520

2580 cgcttcgatc tgaaagccga tgaaggcggt

ctggtgctgc gctttgccca tgacaagccg aaactgacgc gctggtcgga taatgtgcgc 2640

attctcgaag ggctggcgca aaacggcatc atggaggagc aggaagcgca ggcattgacg 2700

ctggcgtaca ccacattgcg tgatgagctg caccacctgg cgctgcaaga gttgccggga 2760

catgtggcgc tctcctgttt tgtcgccgag cgtgcgctta ttaaaaccag ctgggacaag 2820

tggctggtgg aaccgtgcgc cccggcgtaa 2850

<210> 269

<211> 1536

<212> DNA

<213> Kosakonia sacchari

<220>

<223> 16S-3

<220>

<221> modified base

<222> (766)..(776)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (905)..(905)

<223> a, c, t, g, unknown or other

<400> 269

attgaagagt ttgatcatgg ctcagattga acgctggcgg caggcctaac acatgcaagt 60

cgaacggtag cacagagagc ttgctctcgg gtgacgagtg gcggacgggt gagtaatgtc 120

tgggaaactg cctgatggag ggggataact actggaaacg gtagctaata ccgcataacg 180

tcgcaagacc aaagaggggg accttcgggc ctcttgccat cagatgtgcc cagatgggat 240

tagctagtag gtggggtaac ggctcaccta ggcgacgatc cctagctggt ctgagaggat 300

gaccagccac actggaactg agacacggtc cagactccta cgggaggcag cagtggggaa 360

420

gttgtaaagc actttcagcg gggaggaagg gagtaaggtt aataaccttg ctcattgacg 480

ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat acggagggtg 540

caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtctgtc aagtcggatg 600

tgaaatcccc gggctcaacc tgggaactgc atccgaaact ggcaggcttg agtctcgtag 660

agggaggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg aataccggtg 720

780

ggattagata ccctggtagt ccatgccgta aacgatgtct actagccgtt ggggcctttg 840

aggctttagt ggcgcagcta acgcgataag tagaccgcct ggggagtacg gtcgcaagac 900

taaanctcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga 960

tgcaacgcga agaaccttac ctggccttga catagtaaga attttccaga gatggattgg 1020

1080

ttgggttaag tcccgcaacg agcgcaaccc ttgtcattag ttgctacatt tagttgggca 1140

ctctaatgag actgccggtg acaaaccggga ggaaggtggg gatgacgtca agtcctcatg 1200

gcccttatag gtggggctac acacgtcata caatggctgg tacaaagggt tgccaacccg 1260

cgagggggag ctaatcccat aaaaccagtc gtagtccgga tcgcagtctg caactcgact 1320

gcgtgaagtc ggaatcgcta gtaatcgtgg atcagaatgt cacggtgaat acgttcccgg 1380

gtcttgtaca caccgcccgt cacaccatgg gagcgggttc tgccagaagt agttagctta 1440

accgcaagga gggcgattac cacggcaggg ttcgtgactg gggtgaagtc gtaacaaggt 1500

agccgtatcg gaaggtgcgg ctggatcacc tccttt 1536

<210> 270

<211> 882

<212> DNA

<213> Kosakonia sacchari

<220>

<223> nifD1

<400> 270

atgaccatgc gtcaatgcgc catttacggc aaaggtggga tcggcaaatc gaccaccaca 60

cagaacctgg tcgccgcgct ggcggagatg ggtaaaaaag tcatgattgt cggctgtgac 120

ccgaaagccg attccacgcg tttgatcctg catgcgaaag cgcagaacac cattatggag 180

atggctgctg aagtcggctc cgtggaagac ctggagttag aagacgtgct gcaaatcggt 240

tacggcggcg tgcgctgcgc agagtccggc ggcccggagc caggcgtggg ctgtgccggt 300

cgcggggtga tcaccgcgat taacttcctc gaagaagaag gcgcttacgt gccggatctc 360

gtggttgcg gtggtttcgc catgccgatt 420

cgtgaaaaca aagcgcagga gatctacatc gtttgctctg gcgaaatgat ggcgatgtac 480

gccgccaaca acatctccaa aggcatcgtg aaatacgcca aatccggtaa agtgcgcctc 540

ggcgggctga tttgtaactc gcgccagacc gaccgtgaag atgaactgat cattgcgctg 600

gcagaaaaac tcggcacgca gatgatccac tttgttcccc gcgacaacat tgtgcagcgt 660

gcggaaatcc gccgtatgac ggttatcgaa tatgacccga cctgcaatca ggcgaacgaa 720

tatcgcagcc ttgccagcaa aatcgtcaac aacaccaaaa tggtggtgcc caccccctgc 780

accatggatg aactggaaga actgctgatg gagttcggca ttatggatgt ggaagacacc 840

agcatcattg gtaaaaccgc cgccgaagaa aacgccgtct ga 882

<210> 271

<211> 1386

<212> DNA

<213> Kosakonia sacchari

<220>

<223> nifK1

<400> 271

atggccgaaa ttctgcgcag taaaaaaccg ctggcggtca gcccgataaa aagcggccag 60

ccgctggggg cgatcctcgc aagcctgggt gtcgaacagt gcataccgct ggtacacggc 120

gcacagggat gtagcgcgtt cgcgaaggtg ttcttttattc aacattttca cgatccgatc 180

ccgctgcaat cgacggcgat ggatccgact tccaccatta tgggcgccga tgaaaacatt 240

tttaccgcgc tcaatgtgct ctgccagcgc aacgccgcga aagccattgt gctgctcagc 300

accgggcttt cagaagccca gggcagcgac atttcgcggg tggtgcgcca gtttcgtgat 360

gattttcccc ggcataaagg cgttgcgctg ctcaccgtca acacacccga tttctacggc 420

tcgctggaaa acggctacag cgccgtgctg gaaagcatga ttgaacagtg ggtacccgca 480

cagcccgccg ccagcctgcg caaccgccgt gtcaacctgc tggtcagcca tttactgaca 540

ccaggcgata tcgaactgtt gcgcagttat gttgaagcct tcggcctgca accggtgatt 600

gtgccggatc tgtcgctgtc gctggacggg catctggcag acggtgattt ttcgcctgtt 660

acccaagggg gaacatcgct gcgcatgatt gaacagatgg ggcaaaacct ggccaccttt 720

gtgattggcg cctcgctggg ccgtgcggcg gcgttactgg cgcagcgcag ccgtggcgag

gtgatcgccc tgccgcatct gatgacgctt gcagcctgcg acacgtttat tcatcgactg 840

aaaaccctct ccgggcgcga tgtccccgcg tggattgagc gccagcgcgg ccaagttcag 900

gatgcgatga tcgattgcca tatgtggctg cagggtgcgg ctatcgccat ggcagcagaa 960

ggcgatcacc tggcggcatg gtgcgatttc gcccgcagcc agggcatgat ccccggcccg 1020

attgtcgcac cggtcagcca gccggggttg caaaatctgc cggttgaaac cgtggttatc 1080

ggcgatctgg aagatatgca ggatcggctt tgcgcgacgc ccgccgcgtt actggtggcc 1140

1200

ccggtgtatg accggctggg ggaatttcgt cgcctgcgcc aggggtacag cggcattcgt 1260

gacacgctgt ttgagctggc gaatgtgatg cgcgagcgcc atcacccgct tgcaacctac 1320

cgctcgccgc tgcgccagca cgccgacgac aacgttacgc ctggagatct gtatgccgca 1380

tgttaa 1386

<210> 272

<211> 1287

<212> DNA

<213> Kosakonia sacchari

<220>

<223> amtB

<400> 272

atgaaaaaca caacattaaa aacagcgctt gcttcgctgg cgttactgcc tggcctggcg 60

atggcggctc ccgctgtggc ggataaagcc gacaacggct ttatgatgat ttgcaccgcg 120

ctggtgctgt ttatgaccat tccgggcatt gcgctgttct acggcggttt gatccgcggt 180

aaaaacgtgc tgtcgatgct gacgcaggtt gccgtcacct tcgcactggt gtgcattctg 240

tgggtggtgt atggctactc gctggcattt ggcgaggggca acagcttctt cgggagtttt 300

aactgggcga tgttgaaaaa catcgaactg aaagccgtga tgggcagcat ttatcagtat 360

atccacgtgg cgttccaggg ttccttcgcc tgtatcaccg ttggcctgat tgtcggtgca 420

ctggctgagc gtattcgctt ctctgcggtg ctgatttttg tggtggtatg gctgacgctt 480

tcttacgtgc cgattgcaca catggtgtgg ggcggcggtc tgctggcaac ccacggtgcg 540

ctggatttcg caggcggtac ggttgttcac atcaacgctg cgattgcagg tctggtgggg 600

gcttacctga ttggcaaacg cgtgggcttt ggcaaagaag cattcaaacc gcataacctg 660

ccgatggtct tcactggcac cgctatcctg tatgttggct ggtttggttt caacgccggc 720

tccgcaagct cggcgaacga aattgctgcg ctggccttcg tgaacactgt cgttgccact 780

gctgccgcta ttctggcgtg ggtatttggc gaatgggcaa tgcgcggcaa gccgtctctg 840

ctcggtgcct gttctggtgc catcgcgggt ctggttggta tcacccccgc ctgtggttat 900

gtgggtgtcg gcggtgcgct gattgtgggt ctgattgccg gtctggctgg gctgtggggc 960

gttactgcgc tgaaacgtat gttgcgtgtc gatgacccgt gtgacgtatt cggtgtgcac 1020

ggcgtgtgcg gcatcgtggg ctgtatcctg acgggtatct tcgcctctac gtcgctgggt 1080

ggtgtcggtt tcgctgaagg tgtgaccatg ggccatcagg tgctggtgca gctggaaagt 1140

gttgccatca ctatcgtgtg gtctggcgtg gtggccttta ttggttacaa actggcggac 1200

atgacggtag gcctgcgcgt accggaagaa caagaacgtg aagggctgga tgtaaacagc 1260

cacggcgaaa acgcctataa cgcctga 1287

<210> 273

<211> 348

<212> DNA

<213> Kosakonia sacchari

<220>

<223> Prm1

<400> 273

cgtcctgtaa taataaccgg acaattcgga ctgattaaaa aagcgccctt gtggcgcttt 60

ttttatattc ccgcctccat ttaaaataaa aaatccaatc ggatttcact atttaaactg 120

gccattatct aagatgaatc cgatggaagc tcgctgtttt aacacgcgtt ttttaacctt 180

ttattgaaag tcggtgcttc tttgagcgaa cgatcaaatt taagtggatt cccatcaaaa 240

aaatattctc aacctaaaaa agtttgtgta atacttgtaa cgctacatgg agattaactc 300

aatctagagg gtattaataa tgaatcgtac taaactggta ctgggcgc 348

<210> 274

<211> 313

<212> DNA

<213> Kosakonia sacchari

<220>

<223> Prm5

<400> 274

ggacatcatc gcgacaaaca atattaatac cggcaaccac accggcaatt tacgagactg 60

cgcaggcatc ctttctcccg tcaatttctg tcaaataaag taaaagaggc agtctacttg 120

aattacccc ggctggttga gcgtttgttg aaaaaaagta actgaaaaat ccgtagaata 180

gcgccactct gatggttaat taacctattc aattaagaat tatctggatg aatgtgccat 240

taaatgcgca gcataatggt gcgttgtgcg ggaaaactgc ttttttttga aagggttggt 300

cagtagcgga aac 313

<210> 275

<211> 1485

<212> DNA

<213> Rahnella aquatilis

<220>

<223> nifL

<400> 275

atgaccctga atatgatgct cgataacgcc gcgccgggagg ccatcgccgg cgcgctgact 60

caacaacatc cggggctgtt ttttaccatg gtggaacagg cctcggtggc catctccctc 120

accgatgcca gcgccaggat catttacgcc aacccggcgt tttgccgcca gaccggctat 180

tcgctggcgc aattgttaaa ccagaacccg cgcctgctgg ccagcagcca gacgccgcgc 240

gagatctatc aggagatgtg gcataccctg ctccagcgtc agccctggcg cggtcagctg 300

attaatcagc gtcgggacgg cggcctgtac ctggtggaga ttgacatcac cccggtgctt 360

agcccgcaag gggaactgga gcattatctg gcgatgcagc gggatatcag cgtcagctac 420

accctcgaac agcggctgcg caaccatatg accctgatgg aggcggtgct gaataatatc 480

cccgccgccg tggtagtggt ggacgagcag gatcgggtgg tgatggacaa cctcgcctac 540

aaaaccttct gcgctgactg cggcggccgg gagctgctca ccgagctgca ggtctcccct 600

ggccggatga cgcccggcgt ggaggcgatc ctgccggtgg cgctgcgcgg ggccgcgcgc 660

tggctgtcgg taacctgctg gccgttgccc ggcgtcagtg aagaggccag ccgctacttt 720

atcgacagcg cgctggcgcg gaccctggtg gtgatcgccg actgtaccca gcagcgtcag 780

cagcaggagc aagggcgcct tgaccggctg aagcagcaaa tgaccgccgg caagctgctg 840

gcggcgatcc gcgagtcgct ggacgccgcg ctgatccagc tgaactgccc gattaatatg 900

ctggcggcag cccgtcggct gaacggcgag ggaagcggga atgtggcgct ggaggccgcc 960

tggcgtgaag gggaagaggc gatggcgcgg ctccagcgct gtcgcccatc gctggaactc 1020

1080

acacgcttcg atcccgacgg gctgcaggtc gacatggcct caccgcatct gatcggcttt 1140

ggccagcgca ccccactgct ggcgtgctta agcctgtggc tcgatcgcac cctggccctc 1200

gccgccgaac tcccctccgt gccgctggcg atgcagctct acgccgagga gaacgacggc 1260

tggctgtcgc tgtatctgac tgacaacgta ccgctgctgc aggtgcgcta cgctcactcc 1320

cccgacgcgc tgaactcgcc gggcaaaggc atggagctgc ggctgatcca gaccctggtg 1380

gcgcaccatc gcggggccat tgagctggct tcccgaccgc agggcggcac ctgcctgacc 1440

ctgcgtttcc cgctgtttaa caccctgacc ggaggtgaag catga 1485

<210> 276

<211> 1575

<212> DNA

<213> Rahnella aquatilis

<220>

<223> nifA

<400> 276

atgatccctg aatccgaccc ggacaccacc gtcagacgct tcgacctctc tcagcagttc 60

accgccatgc agcggataag cgtggtgctg agccggggcca ccgaggccag caaaacgctg 120

caggaggtgc tcagcgtatt acacaacgat gcctttatgc agcacgggat gatctgcctg 180

tacgacagcg agcaggagat cctcagtatc gaagcgctgc agcaaaccgg ccagcagccc 240

ctccccggca gcacgcagat ccgctatcgc cccggcgagg gactggtggg gaccgtgctg 300

gcccaggggc agtcgctggg gctgccccgg gtcgccgacg atcagcgttt tctcgaccgc 360

ctgagcctct acgattacga tctgccgttt atcgccgtac cgttgatggg gcccaacgcc 420

cggccaatag gggtgctggc ggcccagccg atggcgcgcc aggaagagcg gctgccggcc 480

tgcacccgtt ttctcgaaac cgtcgccaac ctcgtcgccc agaccatccg gctgatgatc 540

cttccggcct cacccgccct gtcgagccgc cagccgccga aggtggaacg gccgccggcc 600

tgctcgtcgt cgcgcggcgt gggccttgac aatatggtcg gcaagagccc ggcgatgcgc 660

cagatcgtgg aggtgatccg tcaggtttcg cgctgggaca ccaccgtgct ggtacgcggc 720

gaaagcggca ccgggaaaga gctgatcgcc aacgccatcc atcaccattc gccacgggct 780

ggcgccgcct tcgtcaaatt taactgcgcg gcgctgccgg acaccctgct ggaaagcgaa 840

ctgttcggcc atgagaaagg cgcctttacc ggggcggtgc gtcagcgtaa aggacgtttt 900

gagctggcgg atggcggcac cctgttcctc gatgagattg gtgaaagcag cgcctcgttc 960

caggccaagc tgctgcgtat cctccaggag ggggagatgg agcggggtcgg cggcgatgag 1020

accctgcggg tgaatgtccg catcatcgcc gccaccaacc gtcacctgga ggaggaggtc 1080

cggctgggcc atttccgcga ggatctctac tatcgtctga acgtgatgcc catcgccctg 1140

cccccgctgc gcgagcgtca ggaggacatc gccgagctgg cgcacttcct ggtgcgcaaa 1200

atcggccagc atcaggggcg cacgctgcgg atcagcgagg gcgcgatccg cctgctgatg 1260

gagtacagct ggccgggtaa cgttcgcgaa ctggagaact gcctcgaacg atcggcggtg 1320

atgtcggaga gtggcctgat cgatcgcgac gtgatcctct tcactcacca ggatcgtccc 1380

gccaaagccc tgcctgccag cgggccagcg gaagacagct ggctggacaa cagcctggac 1440

gaacgtcagc gactgatcgc cgcgctggaa aaagccggct gggtgcaggc caaggcggca 1500

cggctgctgg ggatgacgcc gcgccaggtc gcttatcggga tccagatcat ggatatcacc 1560

ctgccgcgtc tgtag 1575

<210> 277

<211> 1540

<212> DNA

<213> Rahnella aquatilis

<220>

<223> 16S-1

<400> 277

attgaagagt ttgatcatgg ctcagattga acgctggcgg caggcctaac acatgcaagt 60

cgagcggcag cgggaagtag cttgctactt tgccggcgag cggcggacgg gtgagtaatg 120

tctgggaaac tgcctgatgg agggggataa ctactggaaa cggtagctaa taccgcatga 180

cctcgaaaga gcaaagtggg ggatcttcgg acctcacgcc atcggatgtg cccagatggg 240

attagctagt aggtgaggta atggctcacc taggcgacga tccctagctg gtctgagagg 300

atgaccagcc acactggaac tgagacacgg tccagactcc tacgggaggc agcagtgggg 360

aatattgcac aatgggcgca agcctgatgc agccatgccg cgtgtgtgaa gaaggcctta 420

gggttgtaaa gcactttcag cgaggaggaa ggcatcatac ttaatacgtg tggtgattga 480

cgttactcgc agaagaagca ccggctaact ccgtgccagc agccgcggta atacggaggg 540

tgcaagcgtt aatcggaatt actgggcgta aagcgcacgc aggcggtttg ttaagtcaga 600

tgtgaaatcc ccgagcttaa cttgggaact gcatttgaaa ctggcaagct agagtcttgt 660

agaggggggt agaattccag gtgtagcggt gaaatgcgta gagatctgga ggaataccgg 720

tggcgaaggc ggccccctgg acaaagactg acgctcaggt gcgaaagcgt ggggagcaaa 780

caggattaga taccctggta gtccacgctg taaacgatgt cgacttggag gttgtgccct 840

tgaggcgtgg cttccggagc taacgcgtta agtcgaccgc ctggggagta cggccgcaag 900

gttaaaactc aaatgaattg acggggggccc gcacaagcgg tggagcatgt ggtttaattc 960

gatgcaacgc gaagaacctt acctactctt gacatccaga gaatttgcca gagatggcga 1020

agtgccttcg ggaactctga gacaggtgct gcatggctgt cgtcagctcg tgttgtgaaa 1080

1140

gggaactcaa aggagactgc cggtgataaa ccggaggaag gtggggatga cgtcaagtca 1200

tcatggccct tacgagtagg gctacacacg tgctacaatg gcatatacaa agagaagcga 1260

actcgcgaga gcaagcggac ctcataaagt atgtcgtagt ccggattgga gtctgcaact 1320

cgactccatg tgaatacgtt 1380

cccgggcctt gtacacaccg cccgtcacac catgggagtg ggttgcaaaa gaagtaggta 1440

gcttaacctt cgggagggcg cttaccactt tgtgattcat gactggggtg aagtcgtaac 1500

aaggtaaccg taggggaacc tgcggttggga tcacctcctt 1540

<210> 278

<211> 1540

<212> DNA

<213> Rahnella aquatilis

<220>

<223> 16S-2

<220>

<221> modified base

<222> (267)..(267)

<223> a, c, t, g, unknown or other

<400> 278

attgaagagt ttgatcatgg ctcagattga acgctggcgg caggcctaac acatgcaagt 60

cgagcggcat cgggaagtag cttgctactt tgccggcgag cggcggacgg gtgagtaatg 120

tctgggaaac tgcctgatgg agggggataa ctactggaaa cggtagctaa taccgcatga 180

cctcgaaaga gcaaagtggg ggatcttcgg acctcacgcc atcggatgtg cccagatggg 240

attagctagt aggtgaggta atggctnacc taggcgacga tccctagctg gtctgagagg 300

atgaccagcc acactggaac tgagacacgg tccagactcc tacgggaggc agcagtgggg 360

aatattgcac aatgggcgca agcctgatgc agccatgccg cgtgtgtgaa gaaggcctta 420

gggttgtaaa gcactttcag cgaggaggaa ggcatcatac ttaatacgtg tggtgattga 480

cgttactcgc agaagaagca ccggctaact ccgtgccagc agccgcggta atacggaggg 540

tgcaagcgtt aatcggaatt actgggcgta aagcgcacgc aggcggtttg ttaagtcaga 600

tgtgaaatcc ccgagcttaa cttgggaact gcatttgaaa ctggcaagct agagtcttgt 660

agaggggggt agaattccag gtgtagcggt gaaatgcgta gagatctgga ggaataccgg 720

tggcgaaggc ggccccctgg acaaagactg acgctcaggt gcgaaagcgt ggggagcaaa 780

caggattaga taccctggta gtccacgctg taaacgatgt cgacttggag gttgtgccct 840

tgaggcgtgg cttccggagc taacgcgtta agtcgaccgc ctggggagta cggccgcaag 900

gttaaaactc aaatgaattg acggggggccc gcacaagcgg tggagcatgt ggtttaattc 960

gatgcaacgc gaagaacctt acctactctt gacatccaga gaatttgcca gagatggcga 1020

agtgccttcg ggaactctga gacaggtgct gcatggctgt cgtcagctcg tgttgtgaaa 1080

1140

gggaactcaa aggagactgc cggtgataaa ccggaggaag gtggggatga cgtcaagtca 1200

tcatggccct tacgagtagg gctacacacg tgctacaatg gcatatacaa agagaagcga 1260

actcgcgaga gcaagcggac ctcataaagt atgtcgtagt ccggattgga gtctgcaact 1320

cgactccatg tgaatacgtt 1380

cccgggcctt gtacacaccg cccgtcacac catgggagtg ggttgcaaaa gaagtaggta 1440

gcttaacctt cgggagggcg cttaccactt tgtgattcat gactggggtg aagtcgtaac 1500

aaggtaaccg taggggaacc tgcggttggga tcacctcctt 1540

<210> 279

<211> 1540

<212> DNA

<213> Rahnella aquatilis

<220>

<223> 16S-3

<220>

<221> modified base

<222> (455)..(455)

<223> a, c, t, g, unknown or other

<400> 279

attgaagagt ttgatcatgg ctcagattga acgctggcgg caggcctaac acatgcaagt 60

cgagcggcag cgggaagtag cttgctactt tgccggcgag cggcggacgg gtgagtaatg 120

tctgggaaac tgcctgatgg agggggataa ctactggaaa cggtagctaa taccgcatga 180

cctcgaaaga gcaaagtggg ggatcttcgg acctcacgcc atcggatgtg cccagatggg 240

attagctagt aggtgaggta atggctcacc taggcgacga tccctagctg gtctgagagg 300

atgaccagcc acactggaac tgagacacgg tccagactcc tacgggaggc agcagtgggg 360

aatattgcac aatgggcgca agcctgatgc agccatgccg cgtgtgtgaa gaaggcctta 420

gggttgtaaa gcactttcag cgaggaggaa ggcancatac ttaatacgtg tggtgattga 480

cgttactcgc agaagaagca ccggctaact ccgtgccagc agccgcggta atacggaggg 540

tgcaagcgtt aatcggaatt actgggcgta aagcgcacgc aggcggtttg ttaagtcaga 600

tgtgaaatcc ccgagcttaa cttgggaact gcatttgaaa ctggcaagct agagtcttgt 660

agaggggggt agaattccag gtgtagcggt gaaatgcgta gagatctgga ggaataccgg 720

tggcgaaggc ggccccctgg acaaagactg acgctcaggt gcgaaagcgt ggggagcaaa 780

caggattaga taccctggta gtccacgctg taaacgatgt cgacttggag gttgtgccct 840

tgaggcgtgg cttccggagc taacgcgtta agtcgaccgc ctggggagta cggccgcaag 900

gttaaaactc aaatgaattg acggggggccc gcacaagcgg tggagcatgt ggtttaattc 960

gatgcaacgc gaagaacctt acctactctt gacatccaga gaatttgcca gagatggcga 1020

agtgccttcg ggaactctga gacaggtgct gcatggctgt cgtcagctcg tgttgtgaaa 1080

1140

gggaactcaa aggagactgc cggtgataaa ccggaggaag gtggggatga cgtcaagtca 1200

tcatggccct tacgagtagg gctacacacg tgctacaatg gcatatacaa agagaagcga 1260

actcgcgaga gcaagcggac ctcataaagt atgtcgtagt ccggattgga gtctgcaact 1320

cgactccatg tgaatacgtt 1380

cccgggcctt gtacacaccg cccgtcacac catgggagtg ggttgcaaaa gaagtaggta 1440

gcttaacctt cgggagggcg cttaccactt tgtgattcat gactggggtg aagtcgtaac 1500

aaggtaaccg taggggaacc tgcggttggga tcacctcctt 1540

<210> 280

<211> 1540

<212> DNA

<213> Rahnella aquatilis

<220>

<223> 16S-4

<220>

<221> modified base

<222> (70)..(70)

<223> a, c, t, g, unknown or other

<400> 280

attgaagagt ttgatcatgg ctcagattga acgctggcgg caggcctaac acatgcaagt 60

cgagcggcan cgggaagtag cttgctactt tgccggcgag cggcggacgg gtgagtaatg 120

tctgggaaac tgcctgatgg agggggataa ctactggaaa cggtagctaa taccgcatga 180

cctcgaaaga gcaaagtggg ggatcttcgg acctcacgcc atcggatgtg cccagatggg 240

attagctagt aggtgaggta atggcttacc taggcgacga tccctagctg gtctgagagg 300

atgaccagcc acactggaac tgagacacgg tccagactcc tacgggaggc agcagtgggg 360

aatattgcac aatgggcgca agcctgatgc agccatgccg cgtgtgtgaa gaaggcctta 420

gggttgtaaa gcactttcag cgaggaggaa ggcatcacac ttaatacgtg tggtgattga 480

cgttactcgc agaagaagca ccggctaact ccgtgccagc agccgcggta atacggaggg 540

tgcaagcgtt aatcggaatt actgggcgta aagcgcacgc aggcggtttg ttaagtcaga 600

tgtgaaatcc ccgagcttaa cttgggaact gcatttgaaa ctggcaagct agagtcttgt 660

agaggggggt agaattccag gtgtagcggt gaaatgcgta gagatctgga ggaataccgg 720

tggcgaaggc ggccccctgg acaaagactg acgctcaggt gcgaaagcgt ggggagcaaa 780

caggattaga taccctggta gtccacgctg taaacgatgt cgacttggag gttgtgccct 840

tgaggcgtgg cttccggagc taacgcgtta agtcgaccgc ctggggagta cggccgcaag 900

gttaaaactc aaatgaattg acggggggccc gcacaagcgg tggagcatgt ggtttaattc 960

gatgcaacgc gaagaacctt acctactctt gacatccaga gaatttgcca gagatggcga 1020

agtgccttcg ggaactctga gacaggtgct gcatggctgt cgtcagctcg tgttgtgaaa 1080

1140

gggaactcaa aggagactgc cggtgataaa ccggaggaag gtggggatga cgtcaagtca 1200

tcatggccct tacgagtagg gctacacacg tgctacaatg gcatatacaa agagaagcga 1260

actcgcgaga gcaagcggac ctcataaagt atgtcgtagt ccggattgga gtctgcaact 1320

cgactccatg tgaatacgtt 1380

cccgggcctt gtacacaccg cccgtcacac catgggagtg ggttgcaaaa gaagtaggta 1440

gcttaacctt cgggagggcg cttaccactt tgtgattcat gactggggtg aagtcgtaac 1500

aaggtaaccg taggggaacc tgcggttggga tcacctcctt 1540

<210> 281

<211> 1540

<212> DNA

<213> Rahnella aquatilis

<220>

<223> 16S-5

<400> 281

attgaagagt ttgatcatgg ctcagattga acgctggcgg caggcctaac acatgcaagt 60

cgagcggcag cgggaagtag cttgctactt tgccggcgag cggcggacgg gtgagtaatg 120

tctgggaaac tgcctgatgg agggggataa ctactggaaa cggtagctaa taccgcatga 180

cctcgaaaga gcaaagtggg ggatcttcgg acctcacgcc atcggatgtg cccagatggg 240

attagctagt aggtgaggta atggctcacc taggcgacga tccctagctg gtctgagagg 300

atgaccagcc acactggaac tgagacacgg tccagactcc tacgggaggc agcagtgggg 360

aatattgcac aatgggcgca agcctgatgc agccatgccg cgtgtgtgaa gaaggcctta 420

gggttgtaaa gcactttcag cgaggaggaa ggcatcacac ttaatacgtg tgttgattga 480

cgttactcgc agaagaagca ccggctaact ccgtgccagc agccgcggta atacggaggg 540

tgcaagcgtt aatcggaatt actgggcgta aagcgcacgc aggcggtttg ttaagtcaga 600

tgtgaaatcc ccgagcttaa cttgggaact gcatttgaaa ctggcaagct agagtcttgt 660

agaggggggt agaattccag gtgtagcggt gaaatgcgta gagatctgga ggaataccgg 720

tggcgaaggc ggccccctgg acaaagactg acgctcaggt gcgaaagcgt ggggagcaaa 780

caggattaga taccctggta gtccacgctg taaacgatgt cgacttggag gttgtgccct 840

tgaggcgtgg cttccggagc taacgcgtta agtcgaccgc ctggggagta cggccgcaag 900

gttaaaactc aaatgaattg acggggggccc gcacaagcgg tggagcatgt ggtttaattc 960

gatgcaacgc gaagaacctt acctactctt gacatccaga gaatttgcca gagatggcga 1020

agtgccttcg ggaactctga gacaggtgct gcatggctgt cgtcagctcg tgttgtgaaa 1080

1140

gggaactcaa aggagactgc cggtgataaa ccggaggaag gtggggatga cgtcaagtca 1200

tcatggccct tacgagtagg gctacacacg tgctacaatg gcatatacaa agagaagcga 1260

actcgcgaga gcaagcggac ctcataaagt atgtcgtagt ccggattgga gtctgcaact 1320

cgactccatg tgaatacgtt 1380

cccgggcctt gtacacaccg cccgtcacac catgggagtg ggttgcaaaa gaagtaggta 1440

gcttaacctt cgggagggcg cttaccactt tgtgattcat gactggggtg aagtcgtaac 1500

aaggtaaccg taggggaacc tgcggttggga tcacctcctt 1540

<210> 282

<211> 1009

<212> DNA

<213> Rahnella aquatilis

<220>

<223> 16S-6

<220>

<221> modified base

<222> (296)..(296)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (311)..(311)

<223> a, c, t, g, unknown or other

<220>

<221> modified base

<222> (973)..(973)

<223> a, c, t, g, unknown or other

<400> 282

gtagctaata ccgcatgacc tcgaaagagc aaagtggggg atcttcggac ctcacgccat 60

cggatgtgcc cagatgggat tagctagtag gtgaggtaat ggctcaccta ggcgacgatc 120

cctagctggt ctgagaggat gaccagccac actggaactg agacacggtc cagactccta 180

cgggaggcag cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg 240

tgtgtgaaga aggccttagg gttgtaaagc actttcagcg aggaggaagg catcanactt 300

aatacgtgtg ntgattgacg ttactcgcag aagaagcacc ggctaactcc gtgccagcag 360

ccgcggtaat acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag 420

gcggtttgtt aagtcagatg tgaaatcccc gagcttaact tgggaactgc atttgaaact 480

ggcaagctag agtcttgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga 540

gatctggagg aataccggtg gcgaaggcgg ccccctggac aaagactgac gctcaggtgc 600

gaaagcgtgg ggagcaaaca ggattagata ccctggtagt ccacgctgta aacgatgtcg 660

acttggaggt tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct 720

ggggagtacg gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg 780

gagcatgtgg tttaattcga tgcaacgcga agaaccttac ctactcttga catccagaga 840

atttgccaga gatggcgaag tgccttcggg aactctgaga caggtgctgc atggctgtcg 900

tcagctcgtg ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg 960

ttgccagcac gtnatggtgg gaactcaaag gagactgccg gtgataaac 1009

<210> 283

<211> 1519

<212> DNA

<213> Rahnella aquatilis

<220>

<223> 16S-7

<400> 283

attgaagagt ttgatcatgg ctcagattga acgctggcgg caggcctaac acatgcaagt 60

cgagcggcag cgggaagtag cttgctactt tgccggcgag cggcggacgg gtgagtaatg 120

tcctgatgga ggggataact actggaacgg tagctaatac cgcacctcga aagagcaaag 180

tgggggatct tcggacctca cgccatcgga tgtgcccaga tgggattagc tagtaggtga 240

ggtaatggct cacctaggcg acgatcccta gctggtctga gaggatgacc agccacactg 300

gaactgagac acggtccaga ctcctacggg aggcagcagt ggggaatatt gcacaatggg 360

cgcaagcctg atgcagccat gccgcgtgtg tgaagaaggc cttagggttg taaagcactt 420

tcagcgagga ggaaggcatc atacttaata cgtgtggtga ttgacgttac tcgcagaaga 480

agcaccggct aactccgtgc cagcagccgc ggtaatacgg agggtgcaag cttaatcgga 540

attactgggc gtaaagcgca cgcaggcggt tgttaagtca gatgtgaaat ccccgagctt 600

aacttgggaa ctgcatttga aactggcaag ctagagtctt gtagaggggg gtagaattcc 660

aggtgtagcg gtgaaatgcg tagagatctg gaggaatacc ggtggcgaag gcggccccct 720

ggacaaagac tgacgctcag gtgcgaaagc gtggggagca aacagatta ataccctggt 780

agtccacgct gtaacgatgt cgacttggag gttgtgccct gaggcgtggc ttccggagct 840

aacgcgttaa gtcgaccgcc tggggagtac ggccgcaagg ttaaaactca aatgaattga 900

cggggggccg cacaagcggt ggagcatgtg gtttaattcg atgcaacgcg aagaacctta 960

cctactcttg acatccagag aatttgccag agatggcgaa gtgccttcgg gaactctgag 1020

acaggtgctg catggctgtc gtcagctcgt gttgtgaaat gttgggttaa gtcccgcaac 1080

gagcgcaacc cttatccttt gttgccagca cgtaatggtg ggaactcaaa ggagactgcc 1140

ggtgataaac cggaggaagg tggggatgac gtcaagtcat catggccctt acgagtaggg 1200

ctacacacgt gctacaatgg catatacaaa gagaagcgaa ctcgcgagag caagcggacc 1260

tcataaagta tgtcgtagtc cggattggag tctgcaactc gactccatga agtcggaatc 1320

gctagtaatc gtagatcaga atgctacggt gaatacgttc ccgggccttg tacacaccgc 1380

ccgtcacacc atgggagtgg gttgcaaaag aagtaggtag cttaaccttc gggagggcgc 1440

ttaccacttt gtgattcatg actggggtga agtcgtaaca aggtaaccgt aggggaacct 1500

gcggttggat cacctcctt 1519

<210> 284

<211> 882

<212> DNA

<213> Rahnella aquatilis

<220>

<223> nifH1

<400> 284

atgaccatgc gtcaatgcgc tatctacggt aaaggcggta tcggtaaatc caccaccacc 60

cagaatctcg tcgcggccct cgccgagatg ggtaagaaag tgatgatcgt cggctgcgat 120

ccgaaagcgg attccacccg tctgatcctc cacgctaaag cccagaacac catcatggag 180

atggcggcgg aagtgggctc ggtcgaggat ctggagctcg aagacgttct gcaaatcggc 240

tatggcgatg tccgttgcgc cgaatccggc ggcccggagc caggcgtcgg ctgcgccgga 300

360

gatttcgtct tctatgacgt cctcggcgac gtggtctgcg gcggcttcgc tatgccgatc 420

cgcgaaaaca aagccgga gatctacatc gtctgctccg gcgagatgat ggcgatgtat 480

gccgccaaca atatctccaa agggatcgtg aagtacgcca aatccggcaa ggtgcgcctc 540

ggcggcctga tctgtaactc gcgcaaaacc gaccgggaag acgaactgat catcgccctg 600

gcggagaagc ttggcacgca gatgatccac ttcgttcccc gcgacaacat tgtgcagcgc 660

gcggagatcc gccggatgac ggtgatcgag tacgacccga cctgtcagca ggcgaatgaa 720

tatcgtcaac tggcgcagaa gatcgtcaat aacaccaaaa aagtggtgcc gacgccgtgc 780

accatggacg agctggaatc gctgctgatg gagttcggca tcatggaaga agaagacacc 840

agcatcattg gtaaaaccgc cgctgaagaa aacgcggcct ga 882

<210> 285

<211> 1113

<212> DNA

<213> Rahnella aquatilis

<220>

<223> nifH2

<400> 285

atggttagga aaagtagaag taaaaataca aatatagaac taactgaaca tgaccattta 60

ttaataagtc aaataaaaaa gcttaaaaca caaaccactt gcttttttaa taataaagga 120

ggggttggga agactacatt agtagcaaat ttaggagcag agctatcaat aaactttagt 180

gcaaaagttc ttattgtgga tgccgaccct caatgtaatc tcacgcagta tgtattaagt 240

gatgaagaaa ctcaggactt atatgggcaa gaaaatccag atagtattta tacagtaata 300

agaccactat cctttggtaa aggatatgaa agtgacctcc ctataaggca tgtagagaat 360

ttcggttttg acataattgt cggtgaccct agacttgctt tacaggaaga ccttttagct 420

ggagactggc gagatgccaa aggcggtggg atgcgaggaa ttaggacaac ttttgtattt 480

gcagagttaa ttaagaaagc tcgtgagcta aattatgatt ttgttttctt tgacatggga 540

ccatcattag gcgcaatcaa cagggcagta ttactggcaa tggaattctt tgtcgtccca 600

atgtcaatcg atgtattttc actatgggct attaaaaata ttggctccac ggtttcaata 660

tggaaaaaag aattagacac agggattcgg ctctcagagg aacctagcga attatcacaa 720

ttatcacctc aaggaaaact aaagtttctc ggttacgtca cccaacaaca taaagaacgc 780

tctggatacg atacaattca gcttgagaat actgaggaag aaataaaatc gaaacgtcgg 840

gtaaaggcgt atgaagacat tggagaggtg tttccttcta aaattactga gcatctttct 900

aaactttatg catcaaaaga tatgaaccca caccttggag atatacgtca tttaggtagt 960

ttagctccga aatcacaatc acaacacgtt ccgatgatat cagtgtctgg tacaggaaat 1020

tacaccagac ttagaaaaag cgcgcgtgaa ctttatcgag atattgcaag aagatactta 1080

gagaacattc agactgctaa tggcgagaaa tag 1113

<210> 286

<211> 1374

<212> DNA

<213> Rahnella aquatilis

<220>

<223> nifD1

<400> 286

atgaagggaa aggaaattct ggcgctgctg gacgaacccg cctgcgagca caaccagaag 60

caaaaatccg gctgcagcgc ccctaagccc ggcgctaccg ccggcggttg cgccttcgac 120

ggcgcgcaga taacgctcct gcccatcgcc gacgtcgcgc acctggtgca cggccccatc 180

ggctgcgcgg gcagctcgtg ggataaccgc ggcagcgtca gcgccggccc ggccctcaac 240

cggctcggct ttaccaccga tcttaacgaa caggatgtga ttatggggccg cggcgaacgc 300

cgcctgttcc acgccgtgcg tcacatcgtc gaccgctatc atccggcggc ggtctttatc 360

tacaacacct gcgtaccggc gatggagggc gatgacatcg aggcggtctg ccaggccgca 420

cagaccgcca ccggcgtccc ggtcatcgct attgacgccg ccggtttcta cggcagtaaa 480

aatcttggca accgaatggc gggcgacgtg atgctcaggc aggtgattgg ccagcgcgaa 540

ccggccccgt ggccagacaa cacgcccttt gccccggccc agcgccacga tatcggcctg 600

attggcgaat tcaatatcgc cggcgagttc tggcaggtcc agccgctgct cgacgagctg 660

gggatccgcg tcctcggcag cctctccggc gacggccgct ttgccgagat ccagaccctg 720

caccgggcgc aggccaatat gctggtgtgc tcgcgcgcgc tgatcaacgt cgcccggggg 780

ctggagctgc gctacggcac gccgtggttt gaaggcagct tctacgggat ccgcgccacc 840

tccgacgcct tgcgccagct ggcgacgctg ctgggggatg acgacctgcg ccgccgcacc 900

gaggcgctga tcgcccgcga agagcaggcg gcggagcagg ctcttgcgcc gtggcgtgag 960

cagctccgcg ggcgcaaagt gctgctctat accggcggcg tgaaatcctg gtcggtggta 1020

tcggccctgc aggatctcgg catgaccgtg gtggccaccg gcacgcgcaa atccaccgag 1080

gaggacaaac agcggatccg tgagctgatg ggcgacgagg cggtgatgct tgaggagggc 1140

aatgcccgca ccctgctcga cgtggtgtac cgctatcagg ccgacctgat gatcgccggc 1200

ggacgcaata tgtacaccgc ctggaaagcc cggctgccgt ttctcgatat caatcaggag 1260

cgcgagcacg cctacgccgg ctatcagggc atcatcaccc tcgcccgcca gctctgtctg 1320

accctcgcca gccccgtctg gccgcaaacg catacccgcg ccccgtggcg ctag 1374

<210> 287

<211> 1449

<212> DNA

<213> Rahnella aquatilis

<220>

<223> nifD2

<400> 287

atgaccaacg caacaggcga acgtaacctt gcgctcatcc aggaagtcct ggaggtgttt 60

cccgaaaccg cgcgcaaaga gcgcagaaag cacatgatga tcagcgatcc gcagatggag 120

agcgtcggca agtgcattat ctcgaaccgt aaatcgcagc ccggggtgat gaccgtgcgc 180

ggctgcgcct atgcgggctc gaaaaggggtg gtgtttgggc caatcaaaga catggcccat 240

atctcgcacg gccccatcgg ctgcggccag tattcccgcg ccggacggcg caactactat 300

accggcgtca gcggtgtcga cagcttcggc accctgaact tcacctctga ttttcaggag 360

cgcgatattg ttttcggcgg cgataaaaag ctgaccaaac tgatcgaaga gatggagctg 420

ctgttcccgc tgaccaaagg gatcaccatc cagtcggagt gcccggtggg cctgatcggc 480

gatgacatca gcgccgtagc caacgccagc agcaaggcgc tggataaacc ggtgatcccg 540

gtgcgctgcg aaggctttcg cggcgtatcg caatcgctgg gccaccatat cgccaacgac 600

gtggtgcgcg actgggtgct gaacaatcgc gaagggcagc cgtttgccag caccccgtac 660

gatgttgcca tcattggcga ttacaacatc ggcggcgacg cctgggcctc gcgcattctg 720

ctggaagaga tggggctgcg cgtagtggcg cagtggtccg gcgacggcac cctggtggag 780

atggagaaca ccccattcgt taagcttaac ctcgtccact gctaccgttc gatgaactat 840

atcgcccgcc atatggagga gaaacatcag atcccatgga tggaatataa cttcttcggc 900

ccgaccaaaa tcgccgaatc gctgcgcaag atcgccgatc aatttgatga caccattcgc 960

gccaatgcgg aagcggtgat cgccaaatat gaggggcaga tggcggccat catcgccaaa 1020

tatcgcccgc ggctggaggg gcgcaaagtg ctgctgtaca tgggggggct gcggccgcgc 1080

cacgtcatcg gcgcctatga ggatctcggg atggagatca tcgccgccgg ctacgagttt 1140

gcccataacg atgattacga ccgcaccctg ccggacctga aagagggcac cctgctgttt 1200

gacgatgcca gcagctatga gctggaggcc ttcgtcaaag cgctgaaacc tgacctcatc 1260

ggctccggga tcaaagagaa atatatcttc cagaaaatgg gggtgccgtt ccgccagatg 1320

cactcctggg actattccgg cccctatcac ggctatgacg gcttcgccat ctttgcccgc 1380

gatatggata tgaccctgaa caatccggcg tggaacgaac tgactgcccc gtggctgaag 1440

tctgcgtga 1449

<210> 288

<211> 1386

<212> DNA

<213> Rahnella aquatilis

<220>

<223> nifK1

<400> 288

atggcagata ttatccgcag tgaaaaaccg ctggcggtga gcccgattaa aaccgggcaa 60

ccgctcgggg cgatcctcgc cagcctcggg ctggcccagg ccatcccgct ggtcccggc 120

gcccagggct gcagcgcctt cgccaaagtt ttcttttattc agcatttcca tgacccggtg 180

ccgctgcagt cgacggccat ggatccgacc gccacgatca tgggggccga cggcaatatc 240

ttcaccgcgc tcgacaccct ctgccagcgc cacagcccgc aggccatcgt gctgctcagc 300

accggtctgg cggaagcgca gggcagcgat atcgcccggg tggtgcgcca gtttcgcgag 360

gcgcatccgc gccataacgg cgtggcgatc ctcaccgtca ataccccggga tttttttggc 420

tctatggaaa acggctacag cgcggtgatc gagagcgtga tcgagcagtg ggtcgcgccg 480

acgccgcgtc cggggcagcg gccccggcgg gtcaacctgc tggtcagcca cctctgttcg 540

ccaggggata tcgaatggct gggccgctgc gtggaggcct ttggcctgca gccggtgatc 600

ctgccggacc tctcgcagtc aatggatggc cacctcggtg aaggggattt tacgcccctg 660

acccagggcg gcgcctcgct gcgccagatt gcccagatgg gccagagtct gggcagcttc 720

gccattggcg tgtcgctcca gcgggcggca tcgctcctga cccaacgcag ccgcggcgac 780

gtgatcgccc tgccgcatct gatgaccctc gaccattgcg atacctttat ccatcagctg 840

gcgaagatgt ccggacgccg cgtaccggcc tggattgagc gccagcgtgg ccagctgcag 900

gatgcgatga tcgactgcca tatgtggctt cagggccagc gcatggcgat ggcggcggag 960

ggcgacctgc tggcggcgtg gtgtgatttc gcccgcagcc aggggatgca gcccggcccg 1020

ctggtcgccc ccaccagcca ccccagcctg cgccagctgc cggtcgagca agtcgtgccg 1080

ggggatcttg aggatctgca gcagctgctg agccaccaac ccgccgatct gctggtggct 1140

aactctcacg cccgcgatct ggcggagcag tttgccctgc cgctgatccg cgtcggtttt 1200

cccctcttcg accggctcgg tgagtttcgt cgcgtccgcc aggggtacgc cggtatgcga 1260

gatacgctgt ttgaactggc caatctgctg cgcgaccgcc atcaccacac cgccctctac 1320

1380

cattaa 1386

<210> 289

<211> 1563

<212> DNA

<213> Rahnella aquatilis

<220>

<223> nifK2

<400> 289

atgagccaaa cgatcgataa aattcacagc tgttatccgc tgtttgaaca ggatgaatac 60

cagaccctgt tccagaataa aaagaccctt gaagaggcgc acgacgcgca gcgtgtgcag 120

gaggtttttg cctggaccac caccgccgag tatgaagcgc tgaacttcca gcgcgaggcg 180

ctgaccgtcg acccggccaa agcctgccag ccgctcggcg ccgtactctg cgcgctgggg 240

ttcgccggca ccctgccta cgtgcacggc tcccagggct gcgtcgccta ttttcgcacc 300

tactttaacc gccattttaa agagccggtc gcctgcgtct ccgactccat gaccgaggac 360

gcggcggtgt tcggcggcaa caacaacatg aatctgggcc tgcagaatgc cagcgcgctg 420

tataaacccg agattatcgc cgtctccacc acctgtatgg ccgaggtgat cggcgacgat 480

ctgcaggcgt ttatcgccaa cgccaaaaaa gagggatttg ttgacgaccg catcgccatt 540

ccttacgccc atacccccag ctttatcggc agccatgtca ccggctggga caatatgttc 600

660

ctcaatctgg tgaccggatt tgagacctat ctcggcaact tccgcgtgct gaagcggatg 720

atggcgcaga tggatgtccc gtgcagcctg ctctccgacc catcagaggt gctcgacacc 780

cccgccgacg gccattaccg gatgtacgcc ggcggcacca gccagcagga gatcaaaacc 840

gcgccggacg ccattgacac cctgctgctg cagccgtggc agctggtgaa aagcaaaaag 900

gtggttcagg agatgtggaa ccagcccgcc accgaggtgg ccgttccgct gggcctggcc 960

gccaccgacg cgctgctgat gaccgtcagt cagctgaccg gcaaaccgat cgccgacgct 1020

ctgaccctgg agcgcggccg gctggtcgac atgatgctgg attcccacac ctggctgcat 1080

ggcaaaaaat tcggcctcta cggcgatccg gatttcgtga tggggctgac gcgcttcctg 1140

ctggagctgg gctgcgagcc gacggtgatc ctcagtcata acgccaataa acgctggcaa 1200

aaagcgatga agaaaatgct cgatgcctcg ccgtacggtc aggaaagcga agtgttcatc 1260

aactgcgacc tgtggcactt ccggtcgctg atgttcaccc gtcagccgga ctttatgatc 1320

ggtaactcct acggcaagtt tatccagcgc gataccctgg caaagggcaa agccttcgaa 1380

gtgccgctga tccgtctggg ctttccgctg ttcgaccgcc atcatctgca ccgccagacc 1440

acctggggct atgaaggcgc aatgaacatc gtcacgacgc tggtgaacgc cgtgctggaa 1500

1560

taa 1563

<210> 290

<211> 2838

<212> DNA

<213> Rahnella aquatilis

<220>

<223> glnE

<400> 290

atgatgccgc tttctccgca attacagcag cactggcaga cggtcgctga ccgtctgcca 60

gcggattttc ccattgccga actgagccca caggccaggt cggtcatggc gttcagcgat 120

tttgtcgaac agagtgtgat cgcccagccg ggctggctga atgagcttgc ggactcctcg 180

ccggaggcgg aagagtggcg gcattacgag gcctggctgc aggatcgcct gcaggccgtc 240

actgacgaag cggggttgat gcgagagctg cgtctcttcc gccgccagat gatggtccgc 300

atcgcctggg cgcaggcgct gtcgctggtg agcgaagaag agactctgca gcagctgagc 360

gtcctggcgg agaccctgat tgtcgccgcc cgcgactggc tgtacgccgc ctgctgtaag 420

gagtggggaa cgccatgcaa tgccgagggc cagccgcagc cgctgctgat cctcgggatg 480

ggaaagctgg gcggcggcga gctgaacttc tcttccgata tcgatctgat ctttgcctgg 540

cctgagcatg gcgccacccg cggcggccgc cgcgagctgg ataacgccca gttctttacc 600

cgtctggggc agcggctgat caaggccctt gaccagccga cgcaggacgg ctttgtctat 660

cgggttgaca tgcgcctgcg gccgtttggc gacagtgggc cgctggtact cagttttgcg 720

gcgctggaag attattacca ggagcagggt cgggactggg aacgctatgc gatggtgaaa 780

gcgcggatca tgggcgataa cgacggcgtg tacgccagcg agttgcgcgc gatgctccgt 840

cctttcgtct tccgccgtta tatcgacttc agcgtgatcc agtcgctgcg taacatgaaa 900

ggcatgatcg cccgcgaagt gcggcgtcgc gggctgaaag acaacatcaa gctcggcgcc 960

ggcgggatcc gtgaaattga gtttatcgtt caggtctttc aactgatccg cggtggtcgc 1020

gaacctgcac tgcagcagcg cgccctgctg ccgacgctgg cggcgattga tgagctacat 1080

ctgctgccgg aaggcgacgc ggcgctgctg cgcgaggcct atctgttcct gcgccggctg 1140

gaaaacctgc tgcaaagcat caacgatgag cagacccaga ccctgccgca ggatgaactt 1200

aaccgcgcca ggctggcgtg ggggatgcat accgaagact gggagacgct gagcgcgcag 1260

ctggcgagcc agatggccaa cgtgcggcga gtgtttaatg aactgatcgg cgatgatgag 1320

gatcagtccc cggatgagca actggccgag tactggcgcg agctgtggca ggatgcgctg 1380

gaagaagatg acgccagccc ggcgctggcg catttaaacg ataccgaccg ccgtagcgtg 1440

ctggcgctga ttgccgattt tcgtaaagag ctggatcggc gcaccatcgg cccgcgcggc 1500

cgccaggtgc tggatcagct gatgccgcat ctgctgagcg aaatctgctc gcgcgccgat 1560

gcgccgctgc ctctggcgcg gatcacgccg ctgttgaccg ggatcgtcac ccgtaccacc 1620

tatcttgagc tgctgagcga attccccggc gcgctgaagc acctgatcac gctctgcgcg 1680

gcgtcgccga tggtcgccag ccagctggcg cgccacccgc tgctgctgga tgagctgctg 1740

gatcccaaca ccctctatca gccgacggcg accgatgcct atcgcgacga gctgcgccag 1800

tacctgctgc gcgtgccgga agaggatgaa gagcagcagc tggaggcgtt gcgccagttt 1860

aagcaggcgc agcagctgca tatcgcggcg gcggatatcg ctggtaccct gccggtgatg 1920

aaggtcagcg atcacttaac ctggcttgcc gaagcgatcc tcgacgcggt ggtgcagcag 1980

gcatgggggc agatggtcgc tcgctacggc cagccgaccc acctgcacga tcgccagggt 2040

cgcggcttcg ccgtcgtcgg ctacggtaag cttggcggct gggagctggg ctacagctcc 2100

gatctcgatc tggtgttcct ccatgactgc ccggcggagg tgatgaccga cggcgagcgg 2160

gagattgacg gccgtcagtt ctacctgcgg ctggcccagc ggatcatgca cctgttcagc 2220

acccgcacct cgtccggtat tctctacgaa gtggacgccc ggctgcgtcc ttctggcgcg 2280

gcggggatgc tggtcaccac cgccgacgcg tttgctgact atcagcagaa cgaagcctgg 2340

acgtgggaac atcaggcgct ggtgcgcgcc cgcgtggtct atggcgaccc ggcgctgcag 2400

gcgcgctttg acgccattcg tcgcgatatc ctgaccaccc cgcgggaggg gatgaccctg 2460

cagaccgagg ttcgcgagat gcgcgagaag atgcgcgccc accttggcaa caaacatccc 2520

gatcgttttg atatcaaagc cgatgccggc gggatcaccg atattgaatt tattactcag 2580

2640

cgtattcttg agctgctggc gcagaacgac atcatggacg aggaggaggc gcgcgcctta 2700

acgcatgcgt acaccacctt gcgtgatgcg ctccatcacc tggccctgca ggagcagccg 2760

ggacacgtgg cgccagaggc cttcagccgg gagcgtcagc aggtcagcgc cagctggcag 2820

aagtggctga tggcttaa 2838

<210> 291

<211> 449

<212> DNA

<213> Rahnella aquatilis

<220>

<223> Prm4

<400> 291

agtctgaact catcctgcgg cagtcggtga gacgtatttt tgaccaaaga gtgatctaca 60

tcacggaatt ttgtggttgt tgctgcttaa aagggcaaat ctacccttag aatcaactgt 120

tatatcaggg ggattcagag agatattagg aatttgcaca agcgcacaat ttaaccacat 180

catgataacg ccatgtaaaa caaagataaa aaaacaaaat gcagtgactt acatcgcaag 240

caaggcattt tcttatccaa ttgctcaaag tttggccttt catatcgcaa cgaaaatgcg 300

taatatacgc gcccttgcgg acatcagtat ggtcattcct agttcatgcg catcggacac 360

caccagctta caaattgcct gattgcggcc ccgatggccg gtatcactga ccgaccattt 420

cgtgccttat gtcatgcgat gggggctgg 449

<210> 292

<211> 500

<212> DNA

<213> Rahnella aquatilis

<220>

<223> Prm1.2

<400> 292

tgaacatcac tgatgcacaa gctacctatg tcgaagaatt aactaaaaaa ctgcaagatg 60

caggcattcg cgttaaagcc gacttgagaa atgagaagat tggctttaaa attcgcgaac 120

acacgctacg ccgtgttcct tatatgttag tttgtggcga taaagaggtc gaagcaggca 180

aagttgctgt tcgtacccgc cgcggcaaag acttaggaag catggatgtt agcgaagtcg 240

ttgacaaact gctggcggaa atccgcagca gaagtcttca tcaactggag gaataaagta 300

ttaaaggcgg aaaacgagtt caaccggcgc gtcctaatcg cattaacaaa gagattcgcg 360

cgcaagaagt tcgcctcaca ggcgtcgatg gcgagcagat tggtattgtc agtctgaatg 420

aagctcttga aaaagctgag gaagcgggcg tcgatttagt agaaatcagt ccgaatgccg 480

agccgccagt ttgtcgaatc 500

<210> 293

<211> 170

<212> DNA

<213> Rahnella aquatilis

<220>

<223> Prm3.1

<400> 293

tacagtagcg cctctcaaaa atagataaac ggctcatgta cgtgggccgt ttatttttttc 60

tacccataat cgggaaccgg tgttataatg ccgcgccctc atattgtggg gatttcttaa 120

tgacctatcc tgggtcctaa agttgtagtt gacattagcg gagcactaac 170

<210> 294

<211> 142

<212> DNA

<213> Rahnella aquatilis

<220>

<223> Prm6.1

<400> 294

aatttttttt cacaaagcgt agcgttattg aatcgcacat tttaaactgt tggccgctgt

ggaagcgaat attggtgaaa ggtgcggttt taaggccttt ttctttgact ctctgtcgtt 120

acaaagttaa tatgcgcgcc ct 142

<210> 295

<211> 293

<212> DNA

<213> Rahnella aquatilis

<220>

<223> Prm7.1

<400> 295

ttaaaaacgt gaccgagc attaataaac gccacgaaat gtggcgttta tttattcaaa 60

aagtatcttc tttcataaaa agtgctaaat gcagtagcag caaaattggg ataagtccca 120

tggaatacgg ctgttttcgc tgcaattttt aactttttcg taaaaaaaga tgtttctttg 180

agcgaacgat caaaatatag cgttaaccgg caaaaaatta ttctcattag aaaatagttt 240

gtgtaatact tgtaacgcta catggagatt aacttaatct aggggtttt ata 293

<210> 296

<211> 1188

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2

<400> 296

atggcgctca aacagttaat ccgtctgtgt gccgcctcgc cgatggtcgc gacacaactt 60

gcacgtcatc ctttattgct cgatgaactg ctcgacccgc gcacgcttta ccagccgatt 120

gagccgggcg cttaccgcga cgaactgcgt cagtatctga tgcgggtgcc aacagaagac 180

gaagaacagc agcttgaagc cgtgcgccag ttcaaacagg cccagcattt gcgtatcgca 240

gccggggata tttccggggc attgccggtg atgaaagtca gtgaccattt aacctacctt 300

gccgaggcca ttctcgatgt cgtggtgcag catgcgtggg aacaaatggt cgtaaaatac 360

gggcagcccg cgcatcttca gcaccgtgag gggcgcggtt ttgccgtggt cggttacggg 420

aaactcggtg gctgggagct gggttatagc tcagatctgg atctggtctt cctgctcgat 480

tgcgcgccgg aggtgatgac ggacggcgaa cgcagcatcg acggacgtca gttttatctt 540

cggctggcgc agcgcattat gcacttattc agcaccggga catcgtcagg cattctttac 600

gaggttgatc cgcgtctgcg accttccggc gcatccggca tgctggtcag taccattgaa 660

gcgtttgcag attatcaggc caatgaagcc tggacgtggg agcatcaggc gctggttcgc 720

gcgcgcgtgg tttacgggga tccgcaactg acacagcaat ttaacgccac gcgtcgcgac 780

attctttgcc gccagcgcga tggcgacggc ctgcgtaagg aggtccgtga aatgcgcgag 840

aaaatgtatg cccatctggg gagtaaaaaa gcccacgagt ttgatctgaa agccgatccg 900

ggtggcatca cggatattga attcattgca caatacctgg ttctgcgttt cgcgcatgat 960

gagccgaagc tgacgcgctg gtctgataac gtgcggattt ttgaactgat ggcacgatat 1020

gacatcatgc cggaagagga agcgcgccat ctgacgcagg cttatgtgac gctgcgcgat 1080

gaaattcatc atctggcgtt gcaggaacac agcgggaaag tggccgcgga cagctttgct 1140

actgagcgcg cgcagatccg tgccagctgg gcaaagtggc tcggctga 1188

<210> 297

<211> 2188

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> glnE-delta-AR-2 with a 500 bp flanking region.

<400> 297

cggtactgga acagaaatcg gcggatgcgc aggaaatttg ttatgacacg gcctgtctga 60

agtgcaagtt agtgcttact tcctggctgg caacctcagg ctggacgccg tttattgatg 120

ataaatctgc gaagaaactg gacgcttcct tcaaacgttt tgctgacatc atgctcggtc 180

gtaccgcagc ggatctgaaa gaagcctttg cgcagccact gacggaagaa ggttatcgcg 240

atcagctggc gcgcctgaaa cgccagatca ttaccttcca tttgcttgcc ggtgcttacc 300

ctgaaaaaga cgtcgatgcg tatattgccg gctgggtgga cctgcaacag gccatcgttc 360

agcagcaaca cgcctgggag gattcggccc gttctcacgc ggtgatgatg gatgctttct 420

ggttaaacgg gcaacctcgt taactgactg actagcctgg gcaaactgcc cgggcttttt 480

tttgcaagga atctgatttc atggcgctca aacagttaat ccgtctgtgt gccgcctcgc 540

cgatggtcgc gacacaactt gcacgtcatc ctttattgct cgatgaactg ctcgacccgc 600

gcacgcttta ccagccgatt gagccgggcg cttaccgcga cgaactgcgt cagtatctga 660

tgcgggtgcc aacagaagac gaagaacagc agcttgaagc cgtgcgccag ttcaaacagg 720

cccagcattt gcgtatcgca gccggggata tttccggggc attgccggtg atgaaagtca 780

gtgaccattt aacctacctt gccgaggcca ttctcgatgt cgtggtgcag catgcgtggg 840

aacaaatggt cgtaaaatac gggcagcccg cgcatcttca gcaccgtgag gggcgcggtt 900

ttgccgtggt cggttacggg aaactcggtg gctgggagct gggttatagc tcagatctgg 960

atctggtctt cctgctcgat tgcgcgccgg aggtgatgac ggacggcgaa cgcagcatcg 1020

acggacgtca gttttatctt cggctggcgc agcgcattat gcacttattc agcaccggga 1080

catcgtcagg cattctttac gaggttgatc cgcgtctgcg accttccggc gcatccggca 1140

tgctggtcag taccattgaa gcgtttgcag attatcaggc caatgaagcc tggacgtggg 1200

agcatcaggc gctggttcgc gcgcgcgtgg tttacgggga tccgcaactg acacagcaat 1260

ttaacgccac gcgtcgcgac attctttgcc gccagcgcga tggcgacggc ctgcgtaagg 1320

1380

ttgatctgaa agccgatccg ggtggcatca cggatattga attcattgca caatacctgg 1440

ttctgcgttt cgcgcatgat gagccgaagc tgacgcgctg gtctgataac gtgcggattt 1500

ttgaactgat ggcacgatat gacatcatgc cggaagagga agcgcgccat ctgacgcagg 1560

cttatgtgac gctgcgcgat gaaattcatc atctggcgtt gcaggaacac agcgggaaag 1620

tggccgcgga cagctttgct actgagcgcg cgcagatccg tgccagctgg gcaaagtggc 1680

tcggctgagg gtttttattc ggctaacagg cgcttgtgat attatccggc gcattgtatt 1740

tacccgattt gatttatctg ttttggagtc ttgggatgaa agtgactttg cctgattttc 1800

accgcgcagg tgtgctggtt gtcggtgacg taatgttaga ccgttactgg tatggcccga 1860

ccaatcgtat ttctccggaa gctccggtgc cggtggtgaa ggtcagtacc attgaagagc 1920

ggcctggcgg tgcagctaac gtggcgatga acatttcatc tctgggcgcc tcttcctgtc 1980

tgatcggcct gaccggcgta gacgacgctg cgcgtgccct cagtgagcgt ctggcagaag 2040

tgaaagttaa ctgcgatttc gtcgcactat ccacacatcc taccatcacc aaactgcgaa 2100

ttttgtcccg taaccagcaa ctgatccgcc tcgactttga ggaaggtttt gaaggcgttg 2160

atctcgagcc gatgctgacc aaaataga 2188

<210> 298

<211> 524

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::null-v1

<400> 298

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gatttcgctg 120

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 180

gcacttgaga cacttttggg cgagaaccac cgtctgctgg agtctgaact catcctgcga 240

tgggggctgg gccgtctctg aagctctcgg tgaacattgt tgcgaggcag gatgcgagct 300

360

aagcggcgtt ttcccgtccg gggaatggca tggagctgcg ccttatccag acgctgatcg 420

cccatcatcg cggttcttta gatctctcgg tccgccctga tggcggcacc ttgctgacgt 480

tacgcctgcc ggtacagcag gttatcaccg gaggcttaaa atga 524

<210> 299

<211> 1524

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::null-v1 with a 500 bp flanking region.

<400> 299

tgtttcgtct cgaggccggg caactgagcg gccccgttga aaccgacctg ggctggcatc 60

tgttgttgtg cgaacaaatt cgcctgccgc aacccttgcc gaaagccgaa gccttaacgc 120

gggtgcgtca gcaactgatt gcccggcaac agaaacatta tcagcgccag tggctgcaac 180

aactgatcaa cgcctgagcc tgttctcctt cttgttgatg cagacgggtt aatgcccgtt 240

ttgcacgaaa aatgcacata aattgcctgc gttgccttat aacagcgcag ggaaatcctg 300

cctccggcct tgtgccacac cgcgctttgc ctggtttgtg gtaaaaactg gcccgctttg 360

catcctgatg cttaaaacac cccgttcaga tcaacctttg ggcagataag cccgcgaaag 420

gcctgcaaat tgcacggtta ttccgggtga gtatatgtgt gatttgggtt ccggcattgc 480

gcaataaagg ggagaaagac atgagcatca cggcgttatc agcatcattt cctgagggga 540

atatcgccag ccgcttgtcg ctgcaacatc cttcactgtt ttataccgtg gttgaacaat 600

cttcggtggc gatttcgctg accgatccgc aggcgcgcat ttgttatgcc aatccggcat 660

tctgccgcca gacgggtttt gcacttgaga cacttttggg cgagaaccac cgtctgctgg 720

agtctgaact catcctgcga tgggggctgg gccgtctctg aagctctcgg tgaacattgt 780

tgcgaggcag gatgcgagct ggttgtgttt tgacattacc gataatgtgc cgcgtgaacg 840

ggtgcgttat gcccgccgg aagcggcgtt ttcccgtccg gggaatggca tggagctgcg 900

ccttatccag acgctgatcg cccatcatcg cggttcttta gatctctcgg tccgccctga 960

tggcggcacc ttgctgacgt tacgcctgcc ggtacagcag gttatcaccg gaggcttaaa 1020

1080

acggcgcttt atcgcatcag cgtggcgctg agtcaggaaa gcaacaccgg gcgcgcactg 1140

gcggcgatcc tcgaagtgct tcacgatcat gcatttatgc aatacggcat ggtgtgtctg 1200

tttgataaag aacgcaatgc actctttgtg gaatccctgc atggcatcga cggcgaaagg 1260

aaaaaagaga cccgccatgt ccgttaccgc atgggggaag gcgtgatcgg cgcggtgatg 1320

agccagcgtc aggcgctggt gttaccgcgc atttcagacg atcagcgttt tctcgaccgc 1380

ctgaatattt acgattacag cctgccgttg attggcgtgc cgatccccgg tgcggataat 1440

cagccatcgg gcgtgctggt ggcacagccg atggcgttgc acgaagaccg gctgactgcc 1500

agtacgcggt ttttagaaat ggtc 1524

<210> 300

<211> 266

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::null-v2

<400> 300

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gatttcgctg 120

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 180

gcacttgaga cacttttggg cgagaaccac cgtctgctgg ttaaagcctg ccggtacagc 240

aggttatcac cggaggctta aaatga 266

<210> 301

<211> 1266

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::null-v2 with a 500 bp flanking region.

<400> 301

tgtttcgtct cgaggccggg caactgagcg gccccgttga aaccgacctg ggctggcatc 60

tgttgttgtg cgaacaaatt cgcctgccgc aacccttgcc gaaagccgaa gccttaacgc 120

gggtgcgtca gcaactgatt gcccggcaac agaaacatta tcagcgccag tggctgcaac 180

aactgatcaa cgcctgagcc tgttctcctt cttgttgatg cagacgggtt aatgcccgtt 240

ttgcacgaaa aatgcacata aattgcctgc gttgccttat aacagcgcag ggaaatcctg 300

cctccggcct tgtgccacac cgcgctttgc ctggtttgtg gtaaaaactg gcccgctttg 360

catcctgatg cttaaaacac cccgttcaga tcaacctttg ggcagataag cccgcgaaag 420

gcctgcaaat tgcacggtta ttccgggtga gtatatgtgt gatttgggtt ccggcattgc 480

gcaataaagg ggagaaagac atgagcatca cggcgttatc agcatcattt cctgagggga 540

atatcgccag ccgcttgtcg ctgcaacatc cttcactgtt ttataccgtg gttgaacaat 600

cttcggtggc gatttcgctg accgatccgc aggcgcgcat ttgttatgcc aatccggcat 660

tctgccgcca gacgggtttt gcacttgaga cacttttggg cgagaaccac cgtctgctgg 720

ttaaagcctg ccggtacagc aggttatcac cggaggctta aaatgaccca gttacctacc 780

gcgggccgg ttatccggcg ctttgatatg tctgcccagt ttacggcgct ttatcgcatc 840

agcgtggcgc tgagtcagga aagcaacacc gggcgcgcac tggcggcgat cctcgaagtg 900

cttcacgatc atgcatttat gcaatacggc atggtgtgtc tgtttgataa agaacgcaat 960

gcactctttg tggaatccct gcatggcatc gacggcgaaa ggaaaaaaga gacccgccat 1020

gtccgttacc gcatggggga aggcgtgatc ggcgcggtga tgagccagcg tcaggcgctg 1080

gtgttaccgc gcatttcaga cgatcagcgt tttctcgacc gcctgaatat ttacgattac 1140

agcctgccgt tgattggcgt gccgatcccc ggtgcggata atcagccatc gggcgtgctg 1200

1260

atggtc 1266

<210> 302

<211> 943

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm4

<400> 302

atgagcatca cggcgttatc agcatcattt cctgagggga atatcgccag ccgcttgtcg 60

ctgcaacatc cttcactgtt ttataccgtg gttgaacaat cttcggtggc gatttcgctg 120

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 180

gcacttgaga cacttttggg cgagaaccac cgtctgctgg agtctgaact catcctgcgg 240

300

tgctgcttaa aagggcaaat ctacccttag aatcaactgt tatatcaggg ggattcagag 360

agatattagg aatttgcaca agcgcacaat ttaaccacat catgataacg ccatgtaaaa 420

caaagataaa aaaacaaaat gcagtgactt acatcgcaag caaggcattt tcttatccaa 480

ttgctcaaag tttggccttt catatcgcaa cgaaaatgcg taatatacgc gcccttgcgg 540

acatcagtat ggtcattcct agttcatgcg catcggacac caccagctta caaattgcct 600

gattgcggcc ccgatggccg gtatcactga ccgaccattt cgtgccttat gtcatgcgat 660

gggggctggg ccgtctctga agctctcggt gaacattgtt gcgaggcagg atgcgagctg 720

gttgtgtttt gacattaccg ataatgtgcc gcgtgaacgg gtgcgttatg cccgccggga 780

agcggcgttt tcccgtccgg ggaatggcat ggagctgcgc cttatccaga cgctgatcgc 840

ccatcatcgc ggttctttag atctctcggt ccgccctgat ggcggcacct tgctgacgtt 900

acgcctgccg gtacagcagg ttatcaccgg aggcttaaaa tga 943

<210> 303

<211> 1943

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> delta-nifL::Prm4 with a 500 bp flanking region.

<400> 303

tgtttcgtct cgaggccggg caactgagcg gccccgttga aaccgacctg ggctggcatc 60

tgttgttgtg cgaacaaatt cgcctgccgc aacccttgcc gaaagccgaa gccttaacgc 120

gggtgcgtca gcaactgatt gcccggcaac agaaacatta tcagcgccag tggctgcaac 180

aactgatcaa cgcctgagcc tgttctcctt cttgttgatg cagacgggtt aatgcccgtt 240

ttgcacgaaa aatgcacata aattgcctgc gttgccttat aacagcgcag ggaaatcctg 300

cctccggcct tgtgccacac cgcgctttgc ctggtttgtg gtaaaaactg gcccgctttg 360

catcctgatg cttaaaacac cccgttcaga tcaacctttg ggcagataag cccgcgaaag 420

gcctgcaaat tgcacggtta ttccgggtga gtatatgtgt gatttgggtt ccggcattgc 480

gcaataaagg ggagaaagac atgagcatca cggcgttatc agcatcattt cctgagggga 540

atatcgccag ccgcttgtcg ctgcaacatc cttcactgtt ttataccgtg gttgaacaat 600

cttcggtggc gatttcgctg accgatccgc aggcgcgcat ttgttatgcc aatccggcat 660

tctgccgcca gacgggtttt gcacttgaga cacttttggg cgagaaccac cgtctgctgg 720

agtctgaact catcctgcgg cagtcggtga gacgtatttt tgaccaaaga gtgatctaca 780

tcacggaatt ttgtggttgt tgctgcttaa aagggcaaat ctacccttag aatcaactgt 840

tatatcaggg ggattcagag agatattagg aatttgcaca agcgcacaat ttaaccacat 900

catgataacg ccatgtaaaa caaagataaa aaaacaaaat gcagtgactt acatcgcaag 960

caaggcattt tcttatccaa ttgctcaaag tttggccttt catatcgcaa cgaaaatgcg 1020

taatatacgc gcccttgcgg acatcagtat ggtcattcct agttcatgcg catcggacac 1080

caccagctta caaattgcct gattgcggcc ccgatggccg gtatcactga ccgaccattt 1140

cgtgccttat gtcatgcgat gggggctggg ccgtctctga agctctcggt gaacattgtt 1200

gcgaggcagg atgcgagctg gttgtgtttt gacattaccg ataatgtgcc gcgtgaacgg 1260

gtgcgttatg cccgcccgga agcggcgttt tcccgtccgg ggaatggcat ggagctgcgc 1320

cttatccaga cgctgatcgc ccatcatcgc ggttctttag atctctcggt ccgccctgat 1380

ggcggcacct tgctgacgtt acgcctgccg gtacagcagg ttatcaccgg aggcttaaaa 1440

tgacccagtt acctaccgcg ggcccggtta tccggcgctt tgatatgtct gcccagttta 1500

1560

cggcgatcct cgaagtgctt cacgatcatg catttatgca atacggcatg gtgtgtctgt 1620

ttgataaaga acgcaatgca ctctttgtgg aatccctgca tggcatcgac ggcgaaagga 1680

1740

gccagcgtca ggcgctggtg ttaccgcgca tttcagacga tcagcgtttt ctcgaccgcc 1800

tgaatattta cgattacagc ctgccgttga ttggcgtgcc gatccccggt gcggataatc 1860

agccatcggg cgtgctggtg gcacagccga tggcgttgca cgaagaccgg ctgactgcca 1920

gtacgcggtttttagaaatg gtc 1943

<210> 304

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 304

tggtgtccgg gcgaacgtcg ccaggtggca caaattgtca gaactacgac acgactaacc 60

gaccgcagga gtgtgcgatg accctgaata tgatgatgga 100

<210> 305

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 305

cggaaaacga gttcaaacgg cgcgtcccaa tcgtattaat ggcgagattc gcgccacggga 60

agttcgctta acaggtctgg aaggcgagca gcttggtatt 100

<210> 306

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 306

cgccagagag ttgaaatcga acatttccgt aataccgcca ttacccagga gccgttctgg 60

ttgcacagcg gaaaacgtta acgaaaggat atttcgcatg 100

<210> 307

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 307

cgggcgaacg tcgccaggtg gcacaaattg tcagaactac gacacgacta accgaccgca 60

ggagtgtgcg atgaccctga atatgatgat ggatgccagc 100

<210> 308

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 308

tcaacctaaa aaagtttgtg taatacttgt aacgctacat ggagattaac tcaatctaga 60

gggtattaat aatgaatcgt actaaactgg tactgggcgc 100

<210> 309

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 309

cgggcgaacg tcgccaggtg gcacaaattg tcagaactac gacacgacta accgaccgca 60

ggagtgtgcg atgaccctga atatgatgat ggatgccagc 100

<210> 310

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 310

aatttttctgc ccaaatggct gggattgttc atttttttgtt tgccttacaa cgagagtgac 60

agtacgcgcg ggtagttaac tcaacatctg accggtcgat 100

<210> 311

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 311

gtaaccaata aaggccacca cgccagacca cacgatagtg atggcaacac tttccagctg 60

caccagcacc tgatggccca tggtcacacc ttcagcgaaa 100

<210> 312

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 312

tggtattgtc agtctgaatg aagctcttga aaaagctgag gaagcgggcg tcgatttagt 60

agaaatcagt ccgaatgccg agccgccagt ttgtcgaatc 100

<210> 313

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 313

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 60

gcacttgaga cacttttgggg cgagaaccac cgtctgctgg 100

<210> 314

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 314

cgggaaccgg tgttataatg ccgcgccctc atattgtggg gatttcttaa tgacctatcc 60

tgggtcctaa agttgtagtt gacattagcg gagcactaac 100

<210> 315

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 315

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 60

gcacttgaga cacttttgggg cgagaaccac cgtctgctgg 100

<210> 316

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 316

tcaacctaaa aaagtttgtg taatacttgt aacgctacat ggagattaac tcaatctaga 60

gggtattaat aatgaatcgt actaaactgg tactgggcgc 100

<210> 317

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 317

gggcgacaaa cggcctggtg gcacaaattg tcagaactac gacacgacta actgaccgca 60

ggagtgtgcg atgaccctga atatgatgat ggatgccggc 100

<210> 318

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 318

gggcgacaaa cggcctggtg gcacaaattg tcagaactac gacacgacta actgaccgca 60

ggagtgtgcg atgaccctga atatgatgat ggatgccggc 100

<210> 319

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 319

taagaattat ctggatgaat gtgccattaa atgcgcagca taatggtgcg ttgtgcggga 60

aaactgcttt tttttgaaag ggttggtcag tagcggaaac 100

<210> 320

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 320

cgccagagag tcgaaatcga acatttccgt aataccgcga ttacccagga gccgttctgg 60

ttgcacagcg gaaaacgtta acgaaaggat atttcgcatg 100

<210> 321

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 321

cgccagagag tcgaaatcga acatttccgt aataccgcga ttacccagga gccgttctgg 60

ttgcacagcg gaaaacgtta acgaaaggat atttcgcatg 100

<210> 322

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 322

gatgatggat gctttctggt taaacgggca acctcgttaa ctgactgact agcctgggca 60

aactgcccgg gctttttttt gcaaggaatc tgatttcatg 100

<210> 323

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 323

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 60

gcacttgaga cacttttgggg cgagaaccac cgtctgctgg 100

<210> 324

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 324

catcggacac caccagctta caaattgcct gattgcggcc ccgatggccg gtatcactga 60

ccgaccattt cgtgccttat gtcatgcgat gggggctggg 100

<210> 325

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 325

tcttcaacaa ctggaggaat aaggtattaa aggcggaaaa cgagttcaaa cggcacgtcc 60

gaatcgtatc aatggcgaga ttcgcgccct ggaagttcgc 100

<210> 326

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 326

tccgggttcg gcttaccccg ccgcgttttg cgcacggtgt cggacaattt gtcataactg 60

cgacacaggga gtttgcgatg accctgaata tgatgctcga 100

<210> 327

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 327

atcgcagcgt ctttgaatat ttccgtcgcc aggcgctggc tgccgagccg ttctggctgc 60

atagtggaaa acgataattt caggccaggg agcccttatg 100

<210> 328

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 328

tccgggttcg gcttaccccg ccgcgttttg cgcacggtgt cggacaattt gtcataactg 60

cgacacaggga gtttgcgatg accctgaata tgatgctcga 100

<210> 329

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 329

tcacttttta gcaaagttgc actggacaaa aggtaccaca attggtgtac tgatactcga 60

cacagcatta gtgtcgattt ttcatataaa ggtaattttg 100

<210> 330

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 330

tccgggttcg gcttaccccg ccgcgttttg cgcacggtgt cggacaattt gtcataactg 60

cgacacaggga gtttgcgatg accctgaata tgatgctcga 100

<210> 331

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 331

gttctccttt gcaatagcag ggaagaggcg ccagaaccgc cagcgttgaa gcagtttgaa 60

cgcgttcagt gtataatccg aaacttaatt tcggtttgga 100

<210> 332

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 332

tccgggttcg gcttaccccg ccgcgttttg cgcacggtgt cggacaattt gtcataactg 60

cgacacaggga gtttgcgatg accctgaata tgatgctcga 100

<210> 333

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 333

gatatgcctg aagtattcaa ttacttaggc atttacttaa cgcaggcagg caattttgat 60

gctgcctatg aagcgtttga ttctgtactt gagcttgatc 100

<210> 334

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 334

tggtattgtc agtctgaatg aagctcttga aaaagctgag gaagcgggcg tcgatttagt 60

agaaatcagt ccgaatgccg agccgccagt ttgtcgaatc 100

<210> 335

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 335

tgcaaattgc acggttattc cgggtgagta tatgtgtgat ttgggttccg gcattgcgca 60

ataaagggga gaaagacatg agcatcacgg cgttatcagc 100

<210> 336

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 336

tcagggctgc ggatgtcggg cgtttcacaa cacaaaatgt tgtaaatgcg acacagccgg 60

gcctgaaacc aggagcgtgt gatgaccttt aatatgatgc 100

<210> 337

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. above connection

<400> 337

cggaaaacga gttcaaacgg cacgtccgaa tcgtatcaat ggcgagattc gcgccgga 60

agttcgctta actggtctgg aaggtgagca gctgggtatt 100

<210> 338

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 338

ttcttggttc tctggagcgc tttatcggca tcctgactga agaatttgca ggcttcttcc 60

caacctggct tgcacccgtg caggtagttg tgatgaacat 100

<210> 339

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 339

gcgatagaac tcacttcacg ccccgaaggg ggaagctgcc tgaccctacg attcccgcta 60

tttcattcac tgaccgggagg ttcaaaatga cccagcgaac 100

<210> 340

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 340

tccctgtgcg ccgcgtcgcc gatggtggcc agccaactgg cgcgctaccc gatcctgctc 60

gatgaactgc tcgacccgaa cacgctctat caaccgacgg 100

<210> 341

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 341

cgttctgtaa taataaccgg acaattcgga ctgattaaaa aagcgccctc gcggcgcttt 60

ttttatattc tcgactccat ttaaaataaa aaatccaatc 100

<210> 342

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 342

aactcacttc acgccccgaa gggggaagct gcctgaccct acgattcccg ctatttcatt 60

cactgaccgg aggttcaaaa tgacccagcg aaccgagtcg 100

<210> 343

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 343

cgcgtcaggt tgaacgtaaa aaagtcggtc tgcgcaaagc acgtcgtcgt ccgcagttct 60

ccaaacgtta attggtttct gcttcggcag aacgattggc 100

<210> 344

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 344

aactcacttc acgccccgaa gggggaagct gcctgaccct acgattcccg ctatttcatt 60

cactgaccgg aggttcaaaa tgacccagcg aaccgagtcg 100

<210> 345

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 345

ccgatcccca tcactgtgtg tcttgtatta cagtgccgct tcgtcggctt cgccggtacg 60

aatacgaatg acgcgttgca gctcagcaac gaaaattttg 100

<210> 346

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 346

ccgtctctga agctctcggt gaacattgtt gcgaggcagg atgcgagctg gttgtgtttt 60

gacattaccg ataatgtgcc gcgtgaacgg gtgcgttatg 100

<210> 347

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 347

tgaacatcac tgatgcacaa gctacctatg tcgaagaatt aactaaaaaa ctgcaagatg 60

caggcattcg cgttaaagcc gacttgagaa atgagaagat 100

<210> 348

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 348

ccgtctctga agctctcggt gaacattgtt gcgaggcagg atgcgagctg gttgtgtttt 60

gacattaccg ataatgtgcc gcgtgaacgg gtgcgttatg 100

<210> 349

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 349

tacagtagcg cctctcaaaa atagataaac ggctcatgta cgtgggccgt ttatttttttc 60

tacccataat cgggaaccgg tgttataatg ccgcgccctc 100

<210> 350

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 350

aactcacttc acaccccgaa gggggaagtt gcctgaccct acgattcccg ctatttcatt 60

cactgaccgg aggttcaaaa tgacccagcg aaccgagtcg 100

<210> 351

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 351

cgtcctgtaa taataaccgg acaattcgga ctgattaaaa aagcgccctt gtggcgcttt 60

ttttatattc ccgcctccat ttaaaataaa aaatccaatc 100

<210> 352

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 352

ggacatcatc gcgacaaaca atattaatac cggcaaccac accggcaatt tacgagactg 60

cgcaggcatc ctttctcccg tcaatttctg tcaaataaag 100

<210> 353

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 353

aactcacttc acaccccgaa gggggaagtt gcctgaccct acgattcccg ctatttcatt 60

cactgaccgg aggttcaaaa tgacccagcg aaccgagtcg 100

<210> 354

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 354

tttaacgatc tgattggcga tgatgaaacg gattcgccgg aagatgcgct ttctgagagc 60

tggcgcgaat tgtggcagga tgcgttgcag gaggaggatt 100

<210> 355

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 355

gcactgaaac acctcatttc cctgtgtgcc gcgtcgccga tggttgccag tcagctggcg 60

cgctacccga tcctgcttga tgaattgctc gacccgaata 100

<210> 356

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 356

gcgctcaaac agttaatccg tctgtgtgcc gcctcgccga tggtcgcgac acaacttgca 60

cgtcatcctt tattgctcga tgaactgctc gacccgcgca 100

<210> 357

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 357

agtctgaact catcctgcgg cagtcggtga gacgtatttt tgaccaaaga gtgatctaca 60

tcacggaatt ttgtggttgt tgctgcttaa aagggcaaat 100

<210> 358

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 358

ccgtctctga agctctcggt gaacattgtt gcgaggcagg atgcgagctg gttgtgtttt 60

gacattaccg ataatgtgcc gcgtgaacgg gtgcgttatg 100

<210> 359

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 359

60

tttaacaccc tgaccgggagg tgaagcatga tccctgaatc 100

<210> 360

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 360

agcgtcaggt accggtcatg attcaccgtg cgattctcgg ttccctggag cgcttcattg 60

gcatcctgac cgaagagttc gctggcttct tcccaacctg 100

<210> 361

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 361

gcgctgaagc acctgatcac gctctgcgcg gcgtcgccga tggtcgccag ccagctggcg 60

cgccacccgc tgctgctgga tgagctgctg gatcccaaca 100

<210> 362

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 362

gcccgctgac cgaccagaac ttccaccttg gactcggcta tacccttggc gtgacggcgc 60

gcgataactg ggactacatc cccattccgg tgatcttacc 100

<210> 363

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 363

60

tttaacaccc tgaccgggagg tgaagcatga tccctgaatc 100

<210> 364

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 364

gctaaagttc tcggctaatc gctgataaca tttgacgcaa tgcgcaataa aagggcatca 60

tttgatgccc ttttgcacg ctttcatacc agaacctggc 100

<210> 365

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 365

60

tttaacaccc tgaccgggagg tgaagcatga tccctgaatc 100

<210> 366

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 366

cgccgtcctc gcagtaccat tgcaaccgac tttacagcaa gaagtgattc tggcacgcat 60

ggaacaaatt cttgccagtc gggctttatc cgatgacgaa 100

<210> 367

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 367

60

tttaacaccc tgaccgggagg tgaagcatga tccctgaatc 100

<210> 368

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 368

tctttagatc tctcggtccg ccctgatggc ggcaccttgc tgacgttacg cctgccggta 60

cagcaggtta tcaccgggagg cttaaaatga cccagttacc 100

<210> 369

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 369

tgaatatcac tgactcacaa gctacctatg tcgaagaatt aactaaaaaa ctgcaagatg 60

caggcattcg cgttaaagcc gacttgagaa atgagaagat 100

<210> 370

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 370

ctggggtcac tggagcgctt tatcggcatc ctgaccgaag aatttgccgg tttcttcccg 60

acctggctgg cccctgttca ggttgtggtg atgaatatca 100

<210> 371

<211> 100

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 100 b.p. below connection

<400> 371

gcaatagaac taactacccg ccctgaaggc ggtacctgcc tgaccctgcg attcccgtta 60

tttcattcac tgaccgggagg cccacgatga cccagcgacc 100

<210> 372

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> disrupted nifL / PinfC gene

<400> 372

tggtgtccgg gcgaacgtcg ccaggtggca caaattgtca gaactacgac acgactaacc 60

gaccgcagga gtgtgcgatg accctgaata tgatgatgga ttcttggttc tctggagcgc 120

tttatcggca tcctgactga agaatttgca ggcttcttcc caacctggct tgcacccgtg 180

caggtagttg tgatgaacat 200

<210> 373

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> PinfC / disrupted nifL gene

<400> 373

cggaaaacga gttcaaacgg cgcgtcccaa tcgtattaat ggcgagattc gcgccacggga 60

agttcgctta acaggtctgg aaggcgagca gcttggtatt gcgatagaac tcacttcacg 120

ccccgaaggg ggaagctgcc tgaccctacg attcccgcta tttcattcac tgaccgggagg 180

ttcaaaatga ccagcgaac 200

<210> 374

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 5'-UTR and ATG / truncated glnE gene

<400> 374

cgccagagag ttgaaatcga acatttccgt aataccgcca ttacccagga gccgttctgg 60

ttgcacagcg gaaaacgtta acgaaaggat atttcgcatg tccctgtgcg ccgcgtcgcc 120

gatggtggcc agccaactgg cgcgctaccc gatcctgctc gatgaactgc tcgacccgaa 180

cacgctctat caaccgacgg 200

<210> 375

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> disrupted nifL / Prm1 gene

<400> 375

cgggcgaacg tcgccaggtg gcacaaattg tcagaactac gacacgacta accgaccgca 60

ggagtgtgcg atgaccctga atatgatgat ggatgccagc cgttctgtaa taataaccgg 120

acaattcgga ctgattaaaa aagcgccctc gcggcgcttt ttttatattc tcgactccat 180

ttaaaataaa aaatccaatc 200

<210> 376

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> Prm1 / disrupted nifL gene

<400> 376

tcaacctaaa aaagtttgtg taatacttgt aacgctacat ggagattaac tcaatctaga 60

gggtattaat aatgaatcgt actaaactgg tactgggcgc aactcacttc acgccccgaa 120

gggggaagct gcctgaccct acgattcccg ctatttcatt cactgaccgg aggttcaaaa 180

tgaccagcg aaccgagtcg 200

<210> 377

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> disrupted nifL / Prm7 gene

<400> 377

cgggcgaacg tcgccaggtg gcacaaattg tcagaactac gacacgacta accgaccgca 60

ggagtgtgcg atgaccctga atatgatgat ggatgccagc cgcgtcaggt tgaacgtaaa 120

aaagtcggtc tgcgcaaagc acgtcgtcgt ccgcagttct ccaaacgtta attggtttct 180

gcttcggcag aacgattggc 200

<210> 378

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> Prm4 / disrupted nifL gene

<400> 378

aatttttctgc ccaaatggct gggattgttc atttttttgtt tgccttacaa cgagagtgac 60

agtacgcgcg ggtagttaac tcaacatctg accggtcgat aactcacttc acgccccgaa 120

gggggaagct gcctgaccct acgattcccg ctatttcatt cactgaccgg aggttcaaaa 180

tgaccagcg aaccgagtcg 200

<210> 379

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> from 5'-UTR to ATG - 4 b.p. amtB gene / disrupted amtB gene

<400> 379

gtaaccaata aaggccacca cgccagacca cacgatagtg atggcaacac tttccagctg 60

caccagcacc tgatggccca tggtcacacc ttcagcgaaa ccgatcccca tcactgtgtg 120

tcttgtatta cagtgccgct tcgtcggctt cgccggtacg aatacgaatg acgcgttgca 180

gctcagcaac gaaaattttg 200

<210> 380

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> Prm1.2 / disrupted nifL gene

<400> 380

tggtattgtc agtctgaatg aagctcttga aaaagctgag gaagcgggcg tcgatttagt 60

agaaatcagt ccgaatgccg agccgccagt ttgtcgaatc ccgtctctga agctctcggt 120

gaacattgtt gcgaggcagg atgcgagctg gttgtgtttt gacattaccg ataatgtgcc 180

gcgtgaacgg gtgcgttatg 200

<210> 381

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> disrupted nifL / Prm1.2 gene

<400> 381

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 60

120

gctacctatg tcgaagaatt aactaaaaaa ctgcaagatg caggcattcg cgttaaagcc 180

gacttgagaa atgagaagat 200

<210> 382

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> Prm3.1 / disrupted nifL gene

<400> 382

cgggaaccgg tgttataatg ccgcgccctc atattgtggg gatttcttaa tgacctatcc 60

tgggtcctaa agttgtagtt gacattagcg gagcactaac ccgtctctga agctctcggt 120

gaacattgtt gcgaggcagg atgcgagctg gttgtgtttt gacattaccg ataatgtgcc 180

gcgtgaacgg gtgcgttatg 200

<210> 383

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> disrupted nifL / Prm3.1 gene

<400> 383

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 60

gcacttgaga cacttttggg cgagaaccac cgtctgctgg tacagtagcg cctctcaaaa 120

atagataaac ggctcatgta cgtgggccgt ttatttttttc tacccataat cgggaaccgg 180

tgttataatg ccgcgccctc 200

<210> 384

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> Prm1 / disrupted nifL gene

<400> 384

tcaacctaaa aaagtttgtg taatacttgt aacgctacat ggagattaac tcaatctaga 60

gggtattaat aatgaatcgt actaaactgg tactgggcgc aactcacttc acaccccgaa 120

gggggaagtt gcctgaccct acgattcccg ctatttcatt cactgaccgg aggttcaaaa 180

tgaccagcg aaccgagtcg 200

<210> 385

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> disrupted nifL / Prm1 gene

<400> 385

gggcgacaaa cggcctggtg gcacaaattg tcagaactac gacacgacta actgaccgca 60

ggagtgtgcg atgaccctga atatgatgat ggatgccggc cgtcctgtaa taataaccgg 120

acaattcgga ctgattaaaa aagcgccctt gtggcgcttt ttttatattc ccgcctccat 180

ttaaaataaa aaatccaatc 200

<210> 386

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> disrupted nifL / Prm5 gene

<400> 386

gggcgacaaa cggcctggtg gcacaaattg tcagaactac gacacgacta actgaccgca 60

ggagtgtgcg atgaccctga atatgatgat ggatgccggc ggacatcatc gcgacaaaca 120

atattaatac cggcaaccac accggcaatt tacgagactg cgcaggcatc ctttctcccg 180

tcaatttctg tcaaataaag 200

<210> 387

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> Prm5 / disrupted nifL gene

<400> 387

taagaattat ctggatgaat gtgccattaa atgcgcagca taatggtgcg ttgtgcggga 60

aaactgcttt tttttgaaag ggttggtcag tagcggaaac aactcacttc acaccccgaa 120

gggggaagtt gcctgaccct acgattcccg ctatttcatt cactgaccgg aggttcaaaa 180

tgaccagcg aaccgagtcg 200

<210> 388

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 5'-UTR and ATG / truncated glnE gene

<400> 388

cgccagagag tcgaaatcga acatttccgt aataccgcga ttacccagga gccgttctgg 60

ttgcacagcg gaaaacgtta acgaaaggat atttcgcatg tttaacgatc tgattggcga 120

tgatgaaacg gattcgccgg aagatgcgct ttctgagagc tggcgcgaat tgtggcagga

tgcgttgcaggaggaggatt 200

<210> 389

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 5'-UTR and ATG / truncated glnE gene

<400> 389

cgccagagag tcgaaatcga acatttccgt aataccgcga ttacccagga gccgttctgg 60

ttgcacagcg gaaaacgtta acgaaaggat atttcgcatg gcactgaaac acctcatttc 120

cctgtgtgcc gcgtcgccga tggttgccag tcagctggcg cgctacccga tcctgcttga 180

tgaattgctc gacccgaata 200

<210> 390

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 5'-UTR and ATG / truncated glnE gene

<400> 390

gatgatggat gctttctggt taaacgggca acctcgttaa ctgactgact agcctgggca 60

aactgcccgg gctttttttt gcaaggaatc tgatttcatg gcgctcaaac agttaatccg 120

tctgtgtgcc gcctcgccga tggtcgcgac acaacttgca cgtcatcctt tattgctcga 180

tgaactgctc gacccgcgca 200

<210> 391

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> disrupted nifL / Prm4 gene

<400> 391

accgatccgc aggcgcgcat ttgttatgcc aatccggcat tctgccgcca gacgggtttt 60

gcacttgaga cacttttggg cgagaaccac cgtctgctgg agtctgaact catcctgcgg 120

180

tgctgcttaa aagggcaaat 200

<210> 392

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> Prm4 / disrupted nifL gene

<400> 392

catcggacac caccagctta caaattgcct gattgcggcc ccgatggccg gtatcactga 60

ccgaccattt cgtgccttat gtcatgcgat gggggctggg ccgtctctga agctctcggt 120

gaacattgtt gcgaggcagg atgcgagctg gttgtgtttt gacattaccg ataatgtgcc 180

gcgtgaacgg gtgcgttatg 200

<210> 393

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> PinfC / disrupted nifL gene

<400> 393

tcttcaacaa ctggaggaat aaggtattaa aggcggaaaa cgagttcaaa cggcacgtcc 60

gaatcgtatc aatggcgaga ttcgcgccct ggaagttcgc gccattgagc tggcttcccg 120

accgcagggc ggcacctgcc tgaccctgcg tttcccgctg tttaacaccc tgaccgggagg 180

tgaagcatga tccctgaatc 200

<210> 394

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> disrupted nifL / PinfC gene

<400> 394

tccgggttcg gcttaccccg ccgcgttttg cgcacggtgt cggacaattt gtcataactg 60

cgacacaggga gtttgcgatg accctgaata tgatgctcga agcgtcaggt accggtcatg 120

attcaccgtg cgattctcgg ttccctggag cgcttcattg gcatcctgac cgaagagttc 180

gctggcttct tcccaacctg 200

<210> 395

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> 5'-UTR and ATG / truncated glnE gene

<400> 395

atcgcagcgt ctttgaatat ttccgtcgcc aggcgctggc tgccgagccg ttctggctgc 60

atagtggaaa acgataattt caggccaggg agcccttatg gcgctgaagc acctgatcac 120

gctctgcgcg gcgtcgccga tggtcgccag ccagctggcg cgccacccgc tgctgctgga 180

tgagctgctg gatccaaca 200

<210> 396

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> disrupted nifL / Prm1.2 gene

<400> 396

tccgggttcg gcttaccccg ccgcgttttg cgcacggtgt cggacaattt gtcataactg 60

cgacacaggga gtttgcgatg accctgaata tgatgctcga gcccgctgac cgaccagaac 120

ttccaccttg gactcggcta tacccttggc gtgacggcgc gcgataactg ggactacatc 180

cccattccgg tgatcttacc 200

<210> 397

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> Prm1.2 / disrupted nifL gene

<400> 397

tcacttttta gcaaagttgc actggacaaa aggtaccaca attggtgtac tgatactcga 60

cacagcatta gtgtcgattt ttcatataaa ggtaattttg gccattgagc tggcttcccg 120

accgcagggc ggcacctgcc tgaccctgcg tttcccgctg tttaacaccc tgaccgggagg 180

tgaagcatga tccctgaatc 200

<210> 398

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> disrupted nifL / Prm6.2 gene

<400> 398

tccgggttcg gcttaccccg ccgcgttttg cgcacggtgt cggacaattt gtcataactg 60

cgacacaggga gtttgcgatg accctgaata tgatgctcga gctaaagttc tcggctaatc 120

180

ctttcatacc agaacctggc 200

<210> 399

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> Prm6.2 / disrupted nifL gene

<400> 399

gttctccttt gcaatagcag ggaagaggcg ccagaaccgc cagcgttgaa gcagtttgaa 60

cgcgttcagt gtataatccg aaacttaatt tcggtttgga gccattgagc tggcttcccg 120

accgcagggc ggcacctgcc tgaccctgcg tttcccgctg tttaacaccc tgaccgggagg 180

tgaagcatga tccctgaatc 200

<210> 400

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> disrupted nifL / Prm8.2 gene

<400> 400

tccgggttcg gcttaccccg ccgcgttttg cgcacggtgt cggacaattt gtcataactg 60

cgacacaggga gtttgcgatg accctgaata tgatgctcga cgccgtcctc gcagtaccat 120

tgcaaccgac tttacagcaa gaagtgattc tggcacgcat ggaacaaatt cttgccagtc 180

gggctttatc cgatgacgaa 200

<210> 401

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> Prm8.2 / disrupted nifL gene

<400> 401

gatatgcctg aagtattcaa ttacttaggc atttacttaa cgcaggcagg caattttgat 60

gctgcctatg aagcgtttga ttctgtactt gagcttgatc gccattgagc tggcttcccg 120

accgcagggc ggcacctgcc tgaccctgcg tttcccgctg tttaacaccc tgaccgggagg 180

tgaagcatga tccctgaatc 200

<210> 402

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> PinfC / disrupted nifL gene

<400> 402

tggtattgtc agtctgaatg aagctcttga aaaagctgag gaagcgggcg tcgatttagt 60

agaaatcagt ccgaatgccg agccgccagt ttgtcgaatc tctttagatc tctcggtccg 120

ccctgatggc ggcaccttgc tgacgttacg cctgccggta cagcaggtta tcaccgggagg 180

cttaaaatga ccagttacc 200

<210> 403

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> disrupted nifL / PinfC gene

<400> 403

tgcaaattgc acggttattc cgggtgagta tatgtgtgat ttgggttccg gcattgcgca 60

ataaagggga gaaagacatg agcatcacgg cgttatcagc tgaatatcac tgactcacaa 120

gctacctatg tcgaagaatt aactaaaaaa ctgcaagatg caggcattcg cgttaaagcc 180

gacttgagaa atgagaagat 200

<210> 404

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> disrupted nifL / PinfC gene

<400> 404

tcagggctgc ggatgtcggg cgtttcacaa cacaaaatgt tgtaaatgcg acacagccgg 60

gcctgaaacc aggagcgtgt gatgaccttt aatatgatgc ctggggtcac tggagcgctt 120

tatcggcatc ctgaccgaag aatttgccgg tttcttcccg acctggctgg cccctgttca 180

ggttgtggtg atgaatatca 200

<210> 405

<211> 200

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic polynucleotide

<220>

<223> PinfC / disrupted nifL gene

<400> 405

cggaaaacga gttcaaacgg cacgtccgaa tcgtatcaat ggcgagattc gcgccgga 60

agttcgctta actggtctgg aaggtgagca gctgggtatt gcaatagaac taactacccg 120

ccctgaaggc ggtacctgcc tgaccctgcg attcccgtta tttcattcac tgaccgggagg 180

cccacgatga cccagcgacc 200

<210> 406

<211> 20

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic primer

<400> 406

caagaagttc gcctcacagg 20

<210> 407

<211> 20

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic primer

<400> 407

tgcctcgcaa caatgttcac 20

<210> 408

<211> 20

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic primer

<400> 408

cgccctcata ttgtggggat 20

<210> 409

<211> 19

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic primer

<400> 409

ggcataacgc acccgttca 19

<210> 410

<211> 15

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic probe

<400> 410

tctgaagctc tcggt 15

<210> 411

<211> 22

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic primer

<400> 411

taaactggta ctgggcgcaa ct 22

<210> 412

<211> 23

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic primer

<400> 412

caaatcgaag cgccagacgg tat 23

<210> 413

<211> 15

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic probe

<400> 413

gaccctacga ttcc 15

<210> 414

<211> 20

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic primer

<400> 414

ggtgcactct ttgcatggtt 20

<210> 415

<211> 20

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic primer

<400> 415

gcgcagtctc gtaaattgcc 20

<210> 416

<211> 15

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic probe

<400> 416

gcgatgaccc tgaat 15

<210> 417

<211> 19

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic primer

<400> 417

ctcggcagca tggacgtaa 19

<210> 418

<211> 21

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic primer

<400> 418

agggtgttaa acagcgggaa a 21

<210> 419

<211> 15

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic probe

<400> 419

tccgaatcgt atcaa 15

<210> 420

<211> 20

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic primer

<400> 420

gagccgttct ggctgcatag 20

<210> 421

<211> 18

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic primer

<400> 421

gccgtcggct gatagagg 18

<210> 422

<211> 15

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic probe

<400> 422

tgaagcacct gatca 15

<210> 423

<211> 20

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic primer

<400> 423

ggaaaacgag ttcaaccggc 20

<210> 424

<211> 20

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence description: Synthetic primer

<400> 424

ggggcggaccg agagatctaa 20

<---

Claims (35)

1. A method for identifying a strain of microorganisms that produces at least one pound of nitrogen per acre per season, said method includes: a) analysis of two or more candidate microorganism strains for colonization capacity and nitrogen production per microorganism per hour; And b) identifying at least one microorganism strain of said two or more candidate microorganism strains that has a product of (i) colonizing ability and (ii) N produced per microorganism per hour of at least about 2.5 x 10 ^-8 mmol N per gram wet weight of plant root tissue per hour, wherein at least one microorganism strain of said two or more candidate microorganism strains has an average colonizing capacity per unit of plant root tissue of at least 1.79×10 ⁵ bacterial cells per gram wet weight of plant root tissue. 2. The method of claim. 1, characterized in that the method includes the analysis of these two or more strains of microorganism candidates in the field. 3. The method according to claim 2, characterized in that said method comprises identifying at least one microorganism strain selected from the group consisting of Kosakonia sacchari, Rahnella aquatilis, Klebsiella varicola and Kosakonia pseudosacchari. 4. The method according to p. 3, characterized in that the specified strain of Kosakonia sacchari contains bacteria deposited under the designation of the patent depositary No. 201701002, 201708004, 201708003 or 201708002. 5. The method according to claim 37, characterized in that said strain of Rahnella aquatilis contains bacteria deposited under accession number PTA-122293. 6. The method according to p. 3, characterized in that the specified strain of Klebsiella varicola contains bacteria deposited under the designation of the patent depositary No. 201712001 or 201712002. 7. The method according to p. 1, characterized in that the method includes the analysis of these two or more strains of microorganism candidates in laboratory and/or greenhouse conditions. 8. The method according to claim 7, characterized in that said method includes the identification of at least one strain of microorganisms selected from the group consisting of Kosakonia sacchari, Klebsiella Varicola, Kosakonia pseudosacchari, Azospirillum lipoferum, Enterobacter sp. and Kluyvera intermedia. 9. The method of claim 1, wherein said method comprises assaying said two or more candidate microorganism strains when the microbial strains are associated with a corn plant. 10. The method of claim 1, wherein said method comprises an analysis of the nitrogen produced using an acetylene reduction assay. 11. The method of claim 1, wherein said method comprises assaying said two or more candidate microorganism strains in the presence of exogenous nitrogen. 12. The method of claim 1, wherein identifying the at least one microorganism strain comprises identifying a genetically engineered microbial strain. 13. A method for increasing nitrogen fixation in non-legume plants in a field, said method comprising applying to said non-legume plants in said field two or more microorganism strains, said two or more microorganism strains comprising microbial strains having the product of (i) colonization ability and ( ii) N produced per microorganism per hour of at least about 2.5 x 10 ^-8 mmol N per gram wet weight of plant root tissue per hour, wherein at least one microorganism strain of said two or more strains of candidate microorganism has an average colonizing capacity per unit of plant root tissue of at least 1.79×10 ⁵ bacterial cells per gram wet weight of plant root tissue. 14. The method of claim 13, wherein said two or more strains of microorganisms in planta produce 5% or more of the fixed nitrogen in said non-legume plant. 15. The method according to claim 13, characterized in that said method comprises the use of at least one strain of microorganisms selected from the group consisting of Kosakonia sacchari, Rahnella aquatilis, Klebsiella varicola and Kosakonia pseudosacchari. 16. The method according to claim 15, wherein said strain of Kosakonia sacchari contains bacteria deposited with the designation of patent depository No. 201701002, 201708004, 201708003 or 201708002. 17. The method of claim 15, wherein said strain of Rahnella aquatilis contains bacteria deposited under accession number PTA-122293. 18. The method according to claim 15, wherein said strain of Klebsiella varicola contains bacteria deposited with the designation of the patent depository No. 201712001 or 201712002. 19. A non-legume crop colonized with a microorganism strain to increase the yield of the non-legume crop, wherein said microbial strain has a product of (i) colonization ability and (ii) N produced per microorganism per hour of at least about 2.5 x 10 ^{- 8} mmol N per gram wet weight of plant root tissue per hour, wherein said microorganism strain produces at least one pound of nitrogen per acre per season, and wherein said microorganism strain has an average colonizing capacity per unit plant root tissue of at least 1.79 x 10 ⁵ bacterial cells per gram wet weight of plant root tissue. 20. The non-legume plant of claim 19, wherein said plant is selected from the group consisting of corn, rice, wheat, barley, sorghum, millet, oats, rye, and triticale. 21. A composition for increasing the yield of a non-legume crop, wherein said composition contains: a) two or more microorganism strains that have a product of (i) colonization ability and (ii) N produced per microorganism per hour of at least about 2.5 x 10 ^-8 mmol N per gram wet weight of plant root tissue per hour wherein said two or more microorganism strains have an average colonizing capacity per unit of plant root tissue of at least 1.79×10 ⁵ bacterial cells per gram wet weight of plant root tissue; And b) non-legume crop seeds, wherein said two or more microorganism strains provide at least one pound of nitrogen per acre per season in the non-legume crop field derived from said crop seed. 22. The composition of claim 21, wherein said composition contains two or more strains of microorganisms. 23. The composition of claim 21, wherein said two or more microorganism strains comprise at least one microbial strain selected from the group consisting of Kosakonia sacchari, Rahnella aquatilis, Klebsiella varicola, and Kosakonia pseudosacchari. 24. The composition according to claim 23, in which the specified strain of Kosakonia sacchari contains bacteria deposited under the designation of the patent depositary No. 201701002, 201708004, 201708003 or 201708002. 25. The composition of claim 23, wherein said strain of Rahnella aquatilis contains bacteria deposited under accession number PTA-122293. 26. The composition according to claim 23, characterized in that said strain of Klebsiella varicola contains bacteria deposited under the designation of the patent depositary No. 201712001 or 201712002. 27. The method according to claim 1 or 13, in which two or more strains of microorganisms are intrageneric. 28. A non-legume plant colonized with a strain of microorganisms according to claim 19, wherein two or more strains of microorganisms are intrageneric. 29. The composition according to claim 21, in which two or more strains of microorganisms are intrageneric.

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