RU2789055C1 - Septa (options) - Google Patents

Abstract

FIELD: laboratory and clinical diagnostics.

SUBSTANCE: the group of inventions relates to laboratory and clinical diagnostics. A septum compatible with plates for genetic analyzers, having a substrate, is disclosed, while 96 recesses with a diameter of each recess of 5.5 mm are formed on the substrate, while the recesses on the outer and inner sides have narrowing in the shape of a cone, in accordance with the wells of the plate, the recesses are located on the substrate in 12 rows of 8 pieces. On the outside of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, the distance from the substrate to the point of narrowing is 2.05 mm, the thickness of the substrate is 0.87 mm. There are also sept options for different number of holes of the plate.

EFFECT: the group of inventions makes it possible to increase the convenience of placing septa in a plate and containers, to ensure tightness when closing the space of wells and containers without changing the general appearance of septa and without deforming them; to eliminate difficulties when passing capillaries through septa, as well as difficulties in moving the autosampler, smoothly dipping into wells and containers; to provide more complete contact when lifting capillaries from plate with septa, providing better cleaning of the capillary from the liquid in the wells of the plate and containers; to protect the composition contained in the plate and buffer from evaporation and splashing.

7 cl, 7 dwg, 7 ex

Description

SUBSTANCE: group of inventions relates to laboratory and clinical diagnostics and can be used for sealing 96-well plates and buffer containers used in genetic analyzers to prevent evaporation and splashing of liquids. Before loading into the genetic analyzer, the 96-well plate or container is covered with a septum through which the analyzer capillaries pass for electrophoresis. The product can be used as a disposable product, but can be reused when cleaning.

Known from EP 1122181 A2 08.08.2001 is a flexible elastomeric septum closure mat for an array of open-top vials for samples held in an array of rows and columns having a predetermined configuration of a first plurality of vials formed in a vial plate having a predetermined configuration, for use in chromatography equipment, including:

a flexible elastomeric sheet having a top and bottom face;

the corresponding second set of closing plugs of the partitions, depending on the specified lower face, having the form of hollow ledges, each of which is provided with a side wall having a proximal upper end and a distal lower end, and a floor covering and integrally connected to the distal end of each side wall, and the upper the proximal end of each side wall is integrally connected to the specified flexible elastomeric sheet; and

a unitary layer of inert elastomer firmly and integrally attached to the bottom edge of said sheet, as well as to the outer surface of each raised floor and sidewall;

wherein said flexible septum may be bent to present a single closure plug for insertion into the corresponding open-top vial, and gradually unfolded to bring successive adjacent closure plugs into alignment for insertion into the corresponding vials until all vials in the array are will receive stoppers inserted into it and retained by elastic cohesion in said vials.

In addition, from the patent EP 3481552 B1 07/01/2020 known self-adhesive layered septa containing: the first outer layer containing or consisting of an elastic copolymer capable of closing, at least partially, the hole formed when the needle is inserted through the layer; and an adhesive second layer for adhering the baffle around or substantially around the mouth area of the well or container to which the baffle is attached, the baffle further comprising a third layer between the first and second layers, said third layer containing or consisting of a thermoplastic and in which the first layer extends substantially continuous over the mouth area except for the vent in the mouth area. A multi-well microplate containing a spaced array of wells, at least one, preferably all of said wells, covered with a self-adhesive layered septum as claimed in any of the preceding claims.

Along with the above, from the application US 2013224868 A1 29.08.2013 a closure for a container containing a reagent is known, the specified closure containing a lid containing at least two through holes and a strip containing a base and at least two partitions, and the specified the base consists of a rigid material, and the specified septa consist of a flexible material, and the specified septa are attached to one surface of the specified base, while each baffle contains a pre-slotted bottom and a barrel forming the sides of the baffle, and the outer diameter of the baffle shaft is greater than the inner diameter of the through holes so that pressure is exerted on the baffle when the baffle is pressed into the through hole.

The disadvantage of previously known septa is:

- complete tightness of wells and containers is not ensured,

- there are difficulties in the passage of capillaries through the septa and difficulties in the movement of the autosampler,

- capillaries are not properly cleaned of liquid,

- sprays and evaporates the composition for research, due to the non-tight fit of the septa.

The task to be solved by the present group of inventions is to improve the convenience of placing septa in the tablet and containers, ensuring tightness when closing the space of wells and containers, without changing the general appearance of the septa and without their deformation; no obstruction in the passage of capillaries through the septa, as well as no obstruction in the movement of the autosampler, smooth immersion into wells and containers, providing more complete contact when lifting capillaries from the plate with a septum providing better cleaning of the capillary from the liquid in the wells of the plate and containers, protection composition, located in the tablet and buffer from evaporation and splashing.

The technical result of the claimed group of inventions is to increase the convenience of placing septa in the tablet and containers, ensuring tightness when closing the space of wells and containers, without changing the general appearance of the septa and without their deformation; no obstruction in the passage of capillaries through the septa, as well as no obstruction in the movement of the autosampler, smooth immersion into wells and containers, providing more complete contact when lifting capillaries from the plate with a septum providing better cleaning of the capillary from the liquid in the wells of the plate and containers, protection composition, located in the tablet and buffer from evaporation and splashing. In addition, there is no effect of septa on electrophoresis parameters during testing, such as: electrophoresis voltage, prephoresis voltage, injection voltage, injection time, electrophoresis time, and current strength, as well as the speed of migration of fragments and nucleotides, resolution of fragments and fluorescence fragments of DNA and nucleotides in the medium of used polymers POP4/7, NimaPOP4/7 NanoPOP 4/7 and PDMA-4/6. In addition, the claimed group of septa is suitable for a wide range of SeqStudio Genetic Analyzers; Genetic Analyzer 3500/3500XL; Applied Biosystems™ 3130/3130xl Genetic Analyzer; Applied Biosystems 3730/3730xl DNA Analyzers; Honor 1616; Nanofor-05 without loss of quality of the conducted researches and is safe when used in the specified genetic analyzers.

The specified technical result is realized due to the following design features of the septa.

Septa on 96-well plates, compatible with genetic analyzers. The septum consists of a substrate in which 96 recesses are made, which are cone-shaped from the outer and inner sides. The recesses are located on the substrate, in 12 rows of 8 pieces, on the ventral side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for free the passage of capillaries and better cleaning of the capillaries, and also prevents evaporation and splashing. The diameter of each recess is 5.5 mm, the depth or distance from the substrate to the constriction of the recess is 2.05 mm, which ensures a snug fit of the septum in the plate. The thickness of the backing is 0.87 mm, which also provides a snug fit.

When the capillaries are lifted from the plate with the septum, there is a tighter contact, which contributes to the high-quality cleaning of the capillary from the reagent. The septum slots are closed to protect the formulation contained in the plate and buffer.

The septum is made of Pentasil (silicone) or polyvinyl chloride.

Septa for the genetic analyzer on 16 holes. The septum consists of a substrate in which 16 recesses are made, which are cone-shaped from the outer and inner sides. The recesses are located on the substrate, in 2 rows of 8 pieces, on the ventral side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for free the passage of capillaries and better cleaning of the capillaries, and also prevents evaporation and splashing. The diameter of each recess is 5.5 mm, the depth or distance from the substrate to the constriction of the recess is 2.05 mm, which ensures a snug fit of the septum in the plate. The thickness of the backing is 2.25 mm, which also ensures a snug fit.

When the capillaries are lifted from the plate with the septum, there is a tighter contact, which contributes to the high-quality cleaning of the capillary from the reagent. The septum slots are closed to protect the formulation contained in the plate and buffer.

The septum is made of Pentasil (silicone) or polyvinyl chloride.

Septa for a container with a cathode buffer for genetic analyzers for 24 wells. The septum has 24 recesses, which are cone-shaped on the outer and inner sides. The recesses are located on the substrate, in 3 rows of 8 pieces, on the ventral side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for free passage capillaries and better cleaning of capillaries, and also prevents evaporation and splashing. The diameter of each recess is 5.5 mm, the depth or distance from the substrate to the site of the recess is 2.05 mm, which ensures a snug fit of the septum in the plate. The thickness of the backing is 2.32 mm, which also provides a snug fit. At a distance of 2.24 mm from the base of the recess in the form of a cone, antennae 5.7 mm long are made, which contributes to a tight fit of the septum in the buffer; when the capillaries rise, the septum does not leave the buffer hole and sits tightly. The septum promotes high-quality cleaning of the capillary from the reagent. The septum slots are closed to protect the composition in the buffer. In addition, the septum is fixed in the buffer with the reverse side of the antennae.

The septum is made of Pentasil (silicone).

Septa for a container with a cathode buffer for genetic analyzers for 48 wells. The septum has 48 recesses, which are cone-shaped from the outer and inner sides. The recesses are located on the substrate, in 6 rows of 8 pieces, on the ventral side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for free passage capillaries and better cleaning of capillaries, and also prevents evaporation and splashing. Each recess is 2.75 mm in diameter and 1.62 mm deep to ensure a snug fit of the septum in the plate. The thickness of the backing is 1.6 mm, which also ensures a snug fit. At a distance of 3.14 mm from the base of the recess, antennae 3.5 mm long are made in the form of a cone, the thickness of the antennae is 0.6 mm, which contributes to a tight fit of the septum in the cathode buffer; when the capillaries rise, the septum does not leave the buffer hole and sits tightly. The septum promotes high-quality cleaning of the capillary from the reagent. The septum slots are closed to protect the composition in the buffer.

The septum is made of Pentasil (silicone) or polyvinyl chloride.

Septa on 96-well plates, compatible with genetic analyzers. The septum has 96 recesses, which are cone-shaped from the outer and inner sides. The recesses are located on the substrate, in 12 rows of 8 pieces, on the ventral side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for free passage capillaries and better cleaning of capillaries, and also prevents evaporation and splashing. Each well is 5.5 mm in diameter and 2.05 mm deep to ensure a tight fit of the septum in the plate. The thickness of the backing is 0.87 mm, which also provides a snug fit. At the same time, the septum is made detachable, for which perforations are applied between the rows, 1.1 mm in size with a distance between them of 4.31 mm. At the same time, the width of the outer rows is 10.16 mm, the inner rows are 7.97 mm.

The septum is made of Pentasil (silicone).

Septa strip for 96-well 8-well plate. The septum has 8 recesses, which are cone-shaped from the outer and inner sides. The recesses are located on the substrate, in 1 row of 8 pieces, on the outer side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for free passage capillaries and better cleaning of capillaries, and also prevents evaporation and splashing. The diameter of each recess is 5.5 mm, the depth or distance from the substrate to the constriction of the recess is 2.05 mm, which ensures a snug fit of the septum in the plate. The thickness of the backing is 0.87 mm, which also provides a snug fit.

The septum is made of Pentasil (silicone) or polyvinyl chloride.

Single septum for 96-well plates. The septum has 1 recess, which is cone-shaped from the outer and inner sides. The recess is located on the substrate, in the amount of 1 piece, on the ventral side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for free passage of capillaries and better cleaning of capillaries, and also prevents evaporation and splashing. The recess diameter is 5.5 mm, the distance from the substrate to the narrowing of the recess is 2.05 mm, which ensures a snug fit of the septum in the plate. The thickness of the backing is 0.87 mm, which also provides a snug fit.

The septum is made of Pentasil (silicone) or polyvinyl chloride.

In FIG. 1 shows a septum on a 96-well plate compatible with genetic analyzers, where 1 is a recess; 2 - substrate; 3 - slot; 4 - cross.

In FIG. 2 shows a septum for a 16-well genetic analyzer, where 1 is a recess; 2 - substrate; 3 - slot; 4 - cross.

In FIG. 3 shows a septum for a 24-well cathode buffer container, where 1 is a recess; 2 - substrate; 3 - slot; 4 - cross, 5 - antennae.

In FIG. 4 shows a septum for a 48-well cathode buffer container, where 1 is a recess; 2 - substrate; 3 - slot; 4 - cross, 5 - antennae, 6 - center.

In FIG. 5 shows a detachable septum on a 96-well plate, where 1 is a recess; 2 - substrate; 3 - slot; 4 - cross, 7 - tear-off grooves, 8 - tear-off direction.

In FIG. 6 shows a septa strip for a 96-well 8-well plate, where 1 is a recess; 2 - substrate; 3 - slot; 4 - cross.

In FIG. 7 shows a single septum for 96-well plates, where 1 is a recess; 2 - substrate; 3 - slot; 4 - cross.

Septa on 96-well plates, compatible with genetic analyzers. The septum consists of a substrate in which 96 recesses are made, which are cone-shaped from the outer and inner sides. The recesses are located on the substrate, in 12 rows of 8 pieces, on the ventral side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for free the passage of capillaries and better cleaning of the capillaries, and also prevents evaporation and splashing. The diameter of each recess is 5.5 mm, the depth or distance from the substrate to the constriction of the recess is 2.05 mm, which ensures a snug fit of the septum in the plate. The thickness of the backing is 0.87 mm, which also provides a snug fit.

When the capillaries are lifted from the plate with the septum, there is a tighter contact, which contributes to the high-quality cleaning of the capillary from the reagent. The septum slots are closed to protect the formulation contained in the plate and buffer.

The septum is made of Pentasil (silicone) or polyvinyl chloride.

Septa for the genetic analyzer on 16 holes. The septum consists of a substrate in which 16 recesses are made, which are cone-shaped from the outer and inner sides. The recesses are located on the substrate, in 2 rows of 8 pieces, on the ventral side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for free the passage of capillaries and better cleaning of the capillaries, and also prevents evaporation and splashing. The diameter of each recess is 5.5 mm, the depth or distance from the substrate to the constriction of the recess is 2.05 mm, which ensures a snug fit of the septum in the plate. The thickness of the backing is 2.25 mm, which also ensures a snug fit.

When the capillaries are lifted from the plate with the septum, there is a tighter contact, which contributes to the high-quality cleaning of the capillary from the reagent. The septum slots are closed to protect the formulation contained in the plate and buffer.

The septum is made of Pentasil (silicone) or polyvinyl chloride.

Septa for a container with a cathode buffer for genetic analyzers for 24 wells. The septum has 24 recesses, which are cone-shaped on the outer and inner sides. The recesses are located on the substrate, in 3 rows of 8 pieces, on the ventral side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for free passage capillaries and better cleaning of capillaries, and also prevents evaporation and splashing. The diameter of each recess is 5.5 mm, the depth or distance from the substrate to the site of the recess is 2.05 mm, which ensures a snug fit of the septum in the plate. The thickness of the backing is 2.32 mm, which also provides a snug fit. At a distance of 2.24 mm from the base of the recess in the form of a cone, antennae 5.7 mm long are made, which contributes to a tight fit of the septum in the buffer; when the capillaries rise, the septum does not leave the buffer hole and sits tightly. The septum promotes high-quality cleaning of the capillary from the reagent. The septum slots are closed to protect the composition in the buffer. In addition, the septum is fixed in the buffer with the reverse side of the antennae.

The septum is made of Pentasil (silicone).

Septa for a container with a cathode buffer for genetic analyzers for 48 wells. The septum has 48 recesses, which are cone-shaped from the outer and inner sides. The recesses are located on the substrate, in 6 rows of 8 pieces, on the ventral side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for free passage capillaries and better cleaning of capillaries, and also prevents evaporation and splashing. Each recess is 2.75 mm in diameter and 1.62 mm deep to ensure a snug fit of the septum in the plate. The thickness of the backing is 1.6 mm, which also ensures a snug fit. At a distance of 3.14 mm from the base of the recess, antennae 3.5 mm long are made in the form of a cone, the thickness of the antennae is 0.6 mm, which contributes to a tight fit of the septum in the cathode buffer; when the capillaries rise, the septum does not leave the buffer hole and sits tightly. The septum promotes high-quality cleaning of the capillary from the reagent. The septum slots close to protect the buffered formulation.

The septum is made of Pentasil (silicone) or polyvinyl chloride.

Septa on 96-well plates, compatible with genetic analyzers. The septum has 96 recesses, which are cone-shaped from the outer and inner sides. The recesses are located on the substrate, in 12 rows of 8 pieces, on the ventral side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for free passage capillaries and better cleaning of capillaries, and also prevents evaporation and splashing. Each well is 5.5 mm in diameter and 2.05 mm deep to ensure a tight fit of the septum in the plate. The thickness of the backing is 0.87 mm, which also provides a snug fit. At the same time, the septum is made detachable, for which perforations are applied between the rows, 1.1 mm in size with a distance between them of 4.31 mm. At the same time, the width of the outer rows is 10.16 mm, the inner rows are 7.97 mm.

The septum is made of Pentasil (silicone).

Septa strip for 96-well 8-well plate. The septum has 8 recesses, which are cone-shaped from the outer and inner sides. The recesses are located on the substrate, in 12 rows of 8 pieces, on the ventral side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for free passage capillaries and better cleaning of capillaries, and also prevents evaporation and splashing. The diameter of each recess is 5.5 mm, the depth or distance from the substrate to the constriction of the recess is 2.05 mm, which ensures a snug fit of the septum in the plate. The thickness of the backing is 0.87 mm, which also provides a snug fit.

The septum is made of Pentasil (silicone) or polyvinyl chloride.

Single septum for 96-well plates. The septum has 1 recess, which is cone-shaped from the outer and inner sides. The recess is located on the substrate, in the amount of 1 piece, on the ventral side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for free passage of capillaries and better cleaning of capillaries, and also prevents evaporation and splashing. The recess diameter is 5.5 mm, the distance from the substrate to the narrowing of the recess is 2.05 mm, which ensures a snug fit of the septum in the plate. The thickness of the backing is 0.87 mm, which also provides a snug fit.

The septum is made of Pentasil (silicone) or polyvinyl chloride.

Example 1

We make a septum with 96 recesses, in accordance with the wells of the tablet from pentasil. On the substrate we form recesses, in the form of a cone both from the outer and inner sides in 12 rows of 8 pieces, on the ventral side of the cone from below, we form a thickening in the form of a cross, on the horizontal line of which we form a slot, the width of which is 1 mm, and the slit length is 3.3 mm, which allows for free passage of capillaries and better cleaning of capillaries, and also prevents evaporation and splashing. We form a recess in the form of a cone with a diameter of 5.5 mm, and the depth is the distance from the substrate to the point of narrowing of the recess-cone equal to 2.05 mm, which ensures a snug fit of the septum in the tablet. The thickness of the backing is 0.87 mm, which also provides a snug fit.

When the capillaries are lifted from the plate with the septum, there is a tighter contact, which contributes to the high-quality cleaning of the capillary from the reagent. The septum slots are closed to protect the formulation contained in the plate and buffer.

Example 2

We make a septum for 16 recesses, which are placed on a polyvinyl chloride substrate. At the same time, we form recesses, in the form of a cone both on the outer and inner sides in 2 rows of 8 pieces, on the upper side of the cone from below, we form a thickening in the form of a cross, on the horizontal line of which we form a slot, the width of which is 1 mm, and the slit length is 3.3 mm, which allows for free passage of capillaries and better cleaning of capillaries, and also prevents evaporation and splashing. The recess is made with a diameter of 5.5 mm, and the depth - the distance from the substrate to the narrowing point of the recess-cone - 2.05 mm, which ensures a snug fit of the septum in the tablet. The thickness of the backing is 2.25 mm, which also ensures a snug fit.

When the capillaries are lifted from the plate with the septum, there is a tighter contact, which contributes to the high-quality cleaning of the capillary from the reagent. The septum slots are closed to protect the formulation contained in the plate and buffer.

Example 3

We make a septum for 24 recesses, which are placed on a substrate of pentasil. At the same time, we form the recesses in the form of a cone both from the outer and inner sides in 3 rows of 8 pieces, on the upper side of the cone from below, we form a thickening in the form of a cross, on the horizontal line of which we form a slot, the width of which is 1 mm, and the length The slit is 3.3 mm, which allows free passage of capillaries and better cleaning of capillaries, as well as prevents evaporation and splashing. The recess is formed with a diameter of 5.5 mm, and a depth - the distance from the substrate to the narrowing of the recess - 2.05 mm, which ensures a snug fit of the septum in the tablet. The thickness of the backing is 2.32 mm, which also provides a snug fit. At the same time, at a distance of 2.24 mm from the base of the recess, we form antennae 5.7 mm long, which contributes to a tight fit of the septum in the buffer; when the capillaries rise, the septum does not leave the buffer hole and sits tightly. The septum promotes high-quality cleaning of the capillary from the reagent. The septum slots are closed to protect the composition in the buffer. In addition, the septum is fixed in the buffer with the reverse side of the antennae.

Example 4

We make a septum for 48 recesses, which are placed on a PVC substrate. At the same time, we form the recesses in the form of a cone both on the outer and inner sides 6 rows of 8 pieces, on the upper side of the cone from below, we form a thickening in the form of a cross, on the horizontal line of which we form a slot, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for free passage of capillaries and better cleaning of capillaries, and also prevents evaporation and splashing. The recess is formed with a diameter of 2.75 mm, and the depth - the distance from the substrate to the place of expansion - is a “cross” of 1.62 mm, which ensures a snug fit of the septum in the tablet. The thickness of the backing is 1.6 mm, which also ensures a snug fit. At the same time, at a distance of 3.14 mm from the base of the recess-cone, we form antennae 3.5 mm long, the thickness of the antennae is 0.6 mm, which contributes to a tight fit of the septum in the cathode buffer; when the capillaries rise, the septum does not leave the buffer hole and sits tightly . The septum promotes high-quality cleaning of the capillary from the reagent. The septum slots are closed to protect the composition in the buffer.

Example 5

We make a detachable septum with 96 recesses, which are placed on a pentasil substrate. At the same time, the recesses are formed in the form of a cone both from the outer and inner sides in 12 rows of 8 pieces, on the upper side of the cone from below, then we form a thickening in the form of a cross, on the horizontal line of which we form a slot, the width of which is 1 mm, and the slot length is 3.3 mm. We form the recess with a diameter of 5.5 mm, and the depth - the distance from the substrate to the narrowing of the recess - 2.05 mm, which ensures a snug fit of the septum in the tablet. The thickness of the backing is 0.87 mm, which also provides a snug fit. At the same time, the septum is made detachable, for which perforations are applied between the rows, 1.1 mm in size with a distance between them of 4.31 mm. At the same time, the width of the outer rows is 10.16, in the inner 7.97 mm.

Example 6

We make a septa-strip from pentasil with 8 recesses, which are placed on the substrate. At the same time, the recesses are formed in the form of a cone both from the outer and from the inner sides in 1 row of 8 pieces. On the outer side of the cone from below, then we form a thickening in the form of a cross, on the horizontal line of which we form a slot, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for the free passage of capillaries and better cleaning of the capillaries, and also prevents evaporation and splashing. The recess is formed with a diameter of 5.5 mm, the depth or distance from the substrate to the narrowing of the recess is 2.05 mm, which ensures a snug fit of the septum in the tablet. The thickness of the backing is 0.87 mm, which also provides a snug fit.

Example 7

We make a single septum with 1 recess, which are placed on a PVC substrate. On the outer side of the cone from below, then we form a thickening in the form of a cross, on the horizontal line of which we form a slot, the width of which is 1 mm, and the length of the slot is 3.3 mm, which allows for the free passage of capillaries and better cleaning of the capillaries, and also prevents evaporation and splashing. The recess diameter is 5.5 mm, the distance from the substrate to the narrowing of the recess is 2.05 mm, which ensures a snug fit of the septum in the plate. The thickness of the backing is 0.87 mm, which also provides a snug fit.

Claims (7)

1. A septum compatible with plates for genetic analyzers, having a substrate, characterized in that 96 recesses are formed on the substrate with a diameter of each recess 5.5 mm, while the recesses on the outer and inner sides have tapered cones in the form of a cone, in accordance with the wells plate, the recesses are located on the substrate in 12 rows of 8 pieces, on the outer side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, the distance from the substrate to the point of constriction is 2.05 mm; the substrate thickness is 0.87 mm. 2. A septa compatible with plates for genetic analyzers, having a substrate, characterized in that 16 recesses are formed on the substrate with a diameter of each recess of 5.5 mm, while the recesses on the outer and inner sides have narrowing in the form of a cone, the recesses are located on the substrate in 2 rows of 8 pieces, on the outer side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, the distance from the substrate to the narrowing point is 2, 05 mm; the substrate thickness is 2.25 mm. 3. A septa compatible with plates for genetic analyzers, having a substrate, characterized in that 24 recesses are formed on the substrate with a diameter of each recess of 5.5 mm, while the recesses on the outer and inner sides have narrowing in the form of a cone, the recesses are located on the substrate in 3 rows of 8 pieces, on the outer side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, the distance from the substrate to the narrowing point is 2, 05 mm; antennae 5.7 mm long are made at a distance of 2.24 mm from the base of the recess; substrate thickness is 2.32mm 4. A septa compatible with plates for genetic analyzers, having a substrate, characterized in that 48 recesses are formed on the substrate with a diameter of each recess of 2.75 mm, while the recesses on the outer and inner sides have cone-shaped constrictions, the recesses are located on the substrate in 6 rows of 8 pieces, on the outer side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which there is a slot, the width of which is 1 mm, and the length of the slot is 3.3 mm, the distance from the substrate to the place of thickening in the form of a cross is 1.62 mm; at a distance of 3.14 mm from the base of the recess, antennae 3.5 mm long are made, the thickness of the antennae is 0.6 mm; the thickness of the substrate is 1.6 mm. 5. A septa compatible with plates for genetic analyzers, having a substrate, characterized in that 96 recesses are formed on the substrate with a diameter of each recess of 5.5 mm, while the recesses on the outer and inner sides have cone-shaped constrictions, the recesses are located on the substrate in 12 rows of 8 pieces, on the outer side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, the distance from the substrate to the narrowing point is 2, 05 mm; the substrate thickness is 0.87 mm; perforations are applied between the rows, 1.1 mm in size with a distance between them of 4.31 mm, while the width of the outer rows is 10.16 mm, in the inner 7.97 mm. 6. A septa compatible with plates for genetic analyzers, having a substrate, characterized in that 8 recesses are formed on the substrate with a diameter of each recess of 5.5 mm, while the recesses on the outer and inner sides have tapering in the form of a cone, the recesses are located on the substrate in 1 row of 8 pieces, on the outer side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, the distance from the substrate to the narrowing point is 2, 05 mm; the substrate thickness is 0.87 mm. 7. A septum compatible with plates for genetic analyzers, having a substrate, characterized in that 1 recess with a diameter of 5.5 mm is formed on the substrate, while the recess on the outer and inner sides has narrowing in the form of a cone, on the outer side of the cone from below, there is a thickening in the form of a cross, on the horizontal line of which a slot is made, the width of which is 1 mm, and the length of the slot is 3.3 mm, the distance from the substrate to the narrowing point is 2.05 mm; the substrate thickness is 0.87 mm.

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