RU2775653C2 - Compositions and methods for changing flowering and architecture of plants for improvement of potential yield - Google Patents

Abstract

FIELD: biochemistry.

SUBSTANCE: invention relates to the field of biochemistry, in particular to a recombinant DNA structure for modulation of floral development in a plant. A transgenic plant and a part of the specified transgenic plant, containing the specified DNA structure, are also disclosed.

EFFECT: invention allows for the effective acceleration of development of a transgenic plant.

29 cl, 13 dwg, 37 tbl, 22 ex

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[1] This application claims priority of U.S. Provisional Application No. 62/410355, filed October 19, 2016, and U.S. Provisional Application No. 62/411408, filed October 21, 2016, both of which are hereby incorporated by reference in their entirety.

FIELD OF TECHNOLOGY

[2] This invention relates to compositions and methods for modulating floral development and vegetative growth through genetic modification of crop plants to increase yield.

INCLUDING A SEQUENCE LIST

[3] The sequence listing contained in a file called P34461WO00_SEQ.txt, which is 177799 bytes in size (defined in MS-Windows®) and created on October 18, 2017, contains 110 nucleotide sequences and was filed with this document in electronic form, and is incorporated by reference in its entirety.

BACKGROUND OF THE INVENTION

[4] The transition from vegetative growth to flowering is an important process during plant development, which is necessary for grain production in cultivated plants. There are several major pathways for regulating flowering time in terrestrial plants that respond to environmental and developmental stimuli, including photoperiodism ( i.e., day length), vernalization ( i.e., response to winter cold), and plant hormones ( i.e. , gibberellins or HA), in addition to autonomous (independent of environmental conditions) pathways. The molecular networks that regulate flowering time in plants include the vernalization and photoperiod pathways. Under inductive photoperiodic conditions, the activity of the CONSTANS (CO) gene in primary leaves increases the expression of the FLOWERING LOCUS T (FT) gene, which translocates to the meristem, triggering the expression of the downstream flowering-activating genes LEAFY (LFY), APETALA1 (AP1), and SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1). The action of other genes, such as FLOWERING LOCUS C (FLC) and TERMINAL FLOWER 1 (TFL1), inhibits the expression or activity of these genes.

[5] With the exception of day-length-neutral plants, most flowering plants are susceptible to diurnal photoperiodic cycles and can be classified as short-day (SD) or long-day (DD) plants based on the photoperiod conditions required to induce flowering. Photoperiod refers to the relative length or duration of light and dark periods in a 24-hour cycle. In general, long-day plants show a tendency to flower when the day length exceeds some threshold photoperiod ( for example , when the days get longer in spring), while short-day plants show a tendency to flower when the day length falls below a threshold photoperiod ( for example , when the days become shorter after the summer solstice). In other words, KD plants bloom when the days get shorter, while DD plants bloom when the days get longer. Soybean is an example of a short day (SD) plant that is induced to flower when the plants are placed under shorter daylight conditions.

[6] Growers are always on the lookout for new ways to influence crop yields, especially to increase the yield of important crop seeds. Thus, there is a continuing need in the art for improved compositions and methods for increasing the yield of various crop plants. This document suggests that improved crop yields can be achieved by improving agronomic performance associated with flowering and generative development.

BRIEF DESCRIPTION OF THE INVENTION

[7] In accordance with one aspect, the present invention provides a recombinant DNA construct comprising a first expression cassette and a second expression cassette, wherein the first expression cassette contains a polynucleotide sequence encoding a florigen FT protein operably linked to a first promoter suitable for expression in plants, and the second expression cassette contains a DNA sequence suitable for transcription encoding an RNA molecule containing a targeting sequence that is at least 80% complementary to at least 15 consecutive nucleotides of the polynucleotide sequence of the first expression cassette, and wherein the DNA sequence suitable for transcription is operably linked with a second promoter suitable for expression in plants.

[8] In accordance with one aspect, the present invention provides a recombinant DNA construct comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a first promoter suitable for expression in plants, wherein the polynucleotide sequence contains a sequence that encodes a target site or sensor in mRNA transcript encoded by the polynucleotide sequence, and wherein the target site of the mRNA transcript is at least 80% complementary to an endogenous RNA molecule, such as an endogenous miRNA or miRNA molecule.

[9] In accordance with one aspect, the present invention provides a recombinant DNA construct comprising a DNA sequence suitable for transcription encoding an RNA molecule containing a targeting sequence that is at least 80% complementary to at least 15 consecutive nucleotides of a polynucleotide sequence, encoding a florigenic FT protein, wherein a DNA sequence suitable for transcription is operably linked to a promoter suitable for expression in plants.

[10] According to one aspect, the present invention provides transgenic plants, plant cells, plant tissues, and plant fragments comprising an insertion of the recombinant DNA construct of the present invention in the genome of such plants, plant cells, plant tissues, and plant fragments.

[11] According to one aspect, the invention provides methods for producing a transgenic plant, comprising (a) transforming at least one explant cell with a recombinant DNA construct of the invention; and (b) regenerating or growing the transgenic plant from the transformed explant. These methods may further comprise (c) selecting a transgenic plant having one or more of the following traits or phenotypes: earlier flowering, longer generative or flowering period, increased number of flowers per node, increased number of flower clusters per node, increased number of pods , pods, pods, fruit, or nuts per node, and an increased number of seeds per node, compared to a control plant not containing the recombinant DNA construct.

[12] According to one aspect, the invention provides methods for planting a transgenic crop plant, comprising planting the transgenic crop plant at increased density in a field, wherein the transgenic crop plant contains an insert of a recombinant DNA construct of the invention.

[13] According to one aspect, the present invention provides a transgenic plant comprising a polynucleotide sequence encoding a polynucleotide sequence encoding a florigenic FT protein operably linked to a first promoter suitable for expression in plants, and a second expression cassette containing a DNA sequence suitable for transcription, an coding RNA molecule containing a targeting sequence that is at least 80% complementary to at least 15 consecutive nucleotides of the polynucleotide sequence of the first expression cassette, and wherein a DNA sequence suitable for transcription is operably linked to a second promoter suitable for expression in plants. In accordance with one aspect, the present invention provides a transgenic plant comprising a DNA sequence suitable for transcription encoding an RNA molecule containing a targeting sequence that is at least 80% complementary to at least 15 consecutive nucleotides of a polynucleotide sequence encoding a florigenic FT protein, wherein a DNA sequence suitable for transcription is operably linked to a promoter suitable for expression in plants.

[14] In one aspect, the present invention provides a transgenic plant that can, on average, have more seeds, pods, pods, fruits, fruits, nuts, or tubers per node than a non-transgenic control plant, e.g., an average of at least 1%, at least 2%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 60%, at least 70%, at least 75 %, at least 80%, at least 90%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200% , at least 250%, at least 300%, at least 350%, or at least 400% more seeds, beans, pods, pods, fruits, nuts, or tubers per node than net genetic control plant. In accordance with one aspect, the transgenic plant may have an average of at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least at least 8, at least 9, or at least 10 more seeds, pods, pods, pods, fruits, nuts, or tubers per node than the non-transgenic control plant. According to one aspect, a transgenic plant may have an average of 2-10, 2-9, 2-8, 2-7, 2-6, 2-5, 2-4, 2-3, 3-10, 3- 9, 3-8, 3-7, 3-6, 3-5, 3-4, 4-10, 4-9, 4-8, 4-7, 4-6 or 4-5 more seeds, beans, pods, pods, fruits, nuts or tubers per node than the non-transgenic control plant. In accordance with one aspect, a transgenic plant may have an average of at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, or at least 10 more seeds, beans, pods, pods, fruits, nuts, or tubers per node compared to a wild-type or non-transgenic control plant. In accordance with one aspect, the transgenic plant can flower for at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7 , at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 20, at least 25, at least 30, at least 35, at least 40, or at least 45 days earlier than the non-transgenic control plant.

[15] According to one aspect, the present invention provides a transgenic plant comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, wherein expression of the florigenic FT protein is downregulated in late vegetative and/or generative tissue.

[16] In accordance with one aspect, the present invention provides a recombinant DNA construct comprising a polynucleotide sequence encoding a florigen FT protein and operably linked to a vegetative stage promoter and at least one sequence encoding an RNA targeting sequence that is complementary to at least a portion of the polynucleotide sequence.

[17] According to one aspect, the present invention provides a transgenic plant comprising a recombinant polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, wherein expression of the polynucleotide sequence is spatially and temporally limited by a small RNA molecule.

[18] In accordance with one aspect, the present invention provides a recombinant DNA construct comprising an expression cassette, wherein the expression cassette contains a polynucleotide sequence encoding a florigenic FT protein operably linked to a promoter, wherein the promoter contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93 %, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% identical to the polynucleotide sequence, selected from the group consisting of SEQ ID NO: 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53 or 54, or a functional part thereof.

BRIEF DESCRIPTION OF GRAPHICS

[19] FIG. 1A illustrates a matrix table that compares the nucleotide sequences for each combination of different FT genes, including their percent identity.

[20] FIG. 1B illustrates a matrix table that compares the protein sequences for each combination of different FT proteins, including their percent identity.

[21] Fig. 1C illustrates multiple alignment of CLUSTAL 2.0.9 sequences for various FT proteins identified as Gm.FT2a with SEQ ID NO: 2, Gm.FT2b with SEQ ID NO: 6, Le.FT with SEQ ID NO: 12, Pt.FT with SEQ ID NO: 20, Os.HD3a with SEQ ID NO: 18, At.FT with SEQ ID NO: 14, At. TSF with SEQ ID NO: 16, Nt.FT with SEQ ID NO: 10, Gm.FT5a with SEQ ID NO: 4 and Zm.ZCN8 with SEQ ID NO: 8.

[22] FIG. 2 illustrates total FT transcript levels in leaf and top tissues of soybeans harvested on days 1, 3 and 5 after growing under short or long daylight conditions.

[23] FIG. 3A-3O and FIG. 4A-4O illustrate the expression profile of the pAt. Erecta promoter by tracking GUS activity in the early stages of soybean development. Fig. 3A-3O are a set of black and white images of dyed fabrics, and the images in FIG. 4A-4O correspond to FIGS. 3A-3O, but selected for blue GUS staining. Fig. 3A-3C and 4A-4C illustrate expression in 3 day old shoots; Fig. 3D-3I and 4D-4I illustrate expression in 10 day old vegetative shoots (grown under 14 hour light/10 hour dark photoperiod conditions); Fig. 3J-3L and 4J-4L illustrate expression in 16 day old generative shoots; and Fig. 3M-3O and 4M-4O illustrate expression in 30 day old mature and immature leaves of the generative shoot. Planks correspond to 100 microns.

[24] FIG. 5A-5F and FIG. 6A-6F illustrate the expression profile of GUS with the pAT. Erecta promoter during R1 and flower stages of development (35-40 days post germination). Fig. 5A-5F are a set of black and white images of dyed fabrics, and the images in FIG. 6A-6F correspond to FIGS. 5A-5F but selected for blue GUS staining. Fig. 5A and 6A illustrate expression in inflorescence stems or stalks (arrows) and FIG. 5B and 6B illustrate expression in pedicels (arrows). Also illustrated is expression in vascular and parenchymal cells (FIGS. 5C and 6C), in filaments (FIGS. 5D and 6D; arrow) and in unpollinated ovules (FIGS. 5E, 5F, 6E and 6F; arrows). Planks correspond to 1 mm.

[25] FIG. 7 illustrates images of shoot apical meristem (SAM) sections from wild-type plants and GmFT2a-expressing transgenic plants 7 days after planting obtained by scanning electron microscopy (SEM).

[26] FIG. 8 illustrates scanning electron microscopy (SEM) micrographs of an axillary inflorescence primordia from a wild-type plant (harvested 27 days after planting) compared to an axillary inflorescence primordia from a Gm.FT2a-expressing transgenic plant (harvested 9 days after planting).

[27] FIG. 9A-9C illustrate the effects of Gm.FT2a expression under the control of the At. Erecta promoter in soy. Fig. 9A illustrates a null segregant showing normal axillary buds, while FIG. 9B and FIG. 9C (corresponding to plants homozygous or hemizygous for the Gm.FT2a transgene, respectively) early flowering and increased number of pods per node compared to the null segregant.

[28] FIG. 10 illustrates a picture of a whole plant of a wild-type null segregant next to plants that are hemizygous and homozygous for the Gm.FT2a transgene as indicated.

[29] FIG. 11 illustrates the image of the main stem of plants that are homozygous or hemizygous for the pAt.Erecta-Gm.FT2a transgene compared to the null segregant as indicated.

[30] FIG. 12 illustrates images of whole plants transformed with pAt.Erecta::Gm.FT2a or pAt.Erecta::Gm.FT2a/pAP1::miRNA-FT2a::T-Apx .

[31] FIG. 13 illustrates images of whole plants transformed with pAt.Erecta::Gm.FT2a or pAt.Erecta::Gm.FT2a + miR172 target site alone.

DETAILED DESCRIPTION OF THE INVENTION

[32] The goal of improving yields is common to all crops in agriculture. This invention includes methods and compositions for improving the yield of flowering (angiosperms) or seed plants by modifying characteristics associated with flowering time, generative development and vegetative growth to improve one or more characteristics or one or more phenotypes associated with flowering and/or yield. such as the number of flowers, seeds and/or pods per plant and/or the number of flowers, seeds and/or pods per node (and/or main stem) of the plant. Without wishing to be bound by theory, it is believed that the compositions and methods of this invention can be used to improve plant yield by increasing the number of floral meristems, increasing the timing of lateral meristem emergence, and/or increasing the time period for pod or seed development in the plant (e.g., duration of the generative period).

[33] It has previously been found that growing short-day plants such as soybeans under long-day conditions (e.g., about 14-16 hours of light per day) and then short-term exposure of these plants to short-day growth conditions (e.g., about 9-11 hours of light per day for about 3-21 days) before returning the plants to long daylight growing conditions (non-inductive), produces plants that have more pods/seeds per plant (and pods/seeds per node and /or on a branch). See, for example, US Pat. No. 8,935,880 and US Patent Application No. 2014/0259905, the entire contents of which are incorporated herein by reference. Artificial inductive "short daylight" conditions during the vegetative stages of development have revealed not only that flowering times can be altered to change one or more yield-related traits or phenotypes (for example, leading to an increase in the number of pods or seeds by plant node), but also that the effect of applying these conditions was dose-dependent when the number of flowers, seeds, and/or pods per plant (and/or plant node) depended on (i) the length of time the short day conditions were applied (i.e., flowering induction signal dose) and (ii) length of photoperiods after short day conditions under long day conditions (i.e., dose or length of vegetative growth inducing signal after short day induction signal). Soybean plants placed under shorter or shorter early short day induction (ESDI) conditions (before returning to long daylight growing conditions) had more flowers, pods, and seeds per plant with more normal plant height and maturity, while plants soybeans placed under longer or longer RIDC conditions produced smaller, earlier dying plants with fewer pods and seeds per plant (though possibly more pods and/or seeds per node).

[34] The short day induction phenotype in soybean was used to identify genes having altered expression in these plants by transcription profiling. These studies identified several genes with altered expression in these treated soybean plants, including the endogenous FT gene, Gm.FT2a , which is overexpressed in response to short day induction conditions. Therefore, it has been suggested that one could use transgenic expression of FT instead of short day induction to increase seed yield, change generative traits or phenotypes in plants, or both. Ectopic expression of the Gm.FT2a transgene or other FT sequence, or a functional fragment, homologue or orthologue thereof, in a flowering or seed-bearing plant can be used to increase seed yield and/or alter one or more generative phenotypes or traits, which may mean an increase in the number of pods/seeds per plant (and/or number of pods/seeds per node or main stem of the plant). As further explained below and depending on the particular plant species, these yield-related or generative phenotypes or traits can also be applied to other botanical structures similar to legumes, such as seed pods, pods, fruits, nuts, tubers, etc. e. Thus, a plant with ectopic expression of the FT sequence may have an increased number of pods, pods, fruits, nuts, tubers, etc. per node(s), main stem and/or branch(s) of the plant, and /or an increased number of seed pods, pods, fruits, nuts, tubers, etc. per plant.

[35] Flowering Locus T (FT) genes play a key role in greater plant growth, and their function is to integrate flowering pathways. In different species, FT proteins have been shown to function as a mobile signal or florigen transmitted from leaves to shoot tips where it initiates generative development. See, for example, Jaeger, KE et al., ʺInterlocking feedback loops govern the dynamic behavior of the floral transition in Arabidopsis ,ʺ The Plant Cell , 25 :820-833 (2013); Corbesier, L et al., ʺFT protein movement contributes to long distance signaling in floral induction of Arabidopsis ,ʺ Science 316 : 1030-1033 (2007); Jaeger, KE et al., "FT protein acts as a long range signal in Arabidopsis ", " Curr Biol 17 : 1050-1054 (2007); and Amasino, RM et al., "The Timing of Flowering," Plant Physiology , 154 (2):516-520 (2010), the entire contents of which are incorporated herein by reference. In Arabidopsis , FT proteins bind to 14-3-3 and Flowering Locus D (FD) proteins in the meristem to form a flowering complex that initiates the activation of key flower meristem identity genes APETATAL1 (AP1) and SOC1 at the shoot apex. See, for example, Taoka, K. et al., ʺ14-3-3 protein act as intracellular receptors for rice Hd3a florigen.ʺ Nature 476 :332-335 (2011). The TERMINAL FLOWER 1 (TFL1) gene is a key FT target repressor that maintains the shoot apical meristem (SAM) center in a vegetative state. TFL1 acts by repressing the LEAFY (LFY) and AP1 genes. Therefore, the relative concentrations of FT and TFL1 in target tissues act in a competitive manner to control the timing of the generative transition of meristems from the vegetative state, which can lead to termination of further vegetative growth. See, for example, Abe, M et al., Science 309 :1052-1055 (2005); and McGarry, R. C. et al., Plant Science 188/189 : 71-81 (2012).

[36] FT genes have been identified in many different species, and ectopic FT expression has been reported to induce early flowering. See, for example, Kong, F. et al., "Two Coordinately Regulated Homologs of Flowering Locus T Are Involved in the Control of Photoperiodic Flowering in Soybean," Plant Physiology 154 : 1220-1231 (2010); Turck, F. et al., ʺRegulation and identity of florigen: Flowering Locus T moves center stage,ʺ Ann Rev Plant Biol 59 : 573-594 (2008); Blackman, BK et al., ʺThe role of recently derived FT paralogs in sunflower domestication,ʺ Curr Biol 20 : 629-635 (2010); Lifschitz, E. et al., ʺThe tomato FT orthologs triggers systemic signals that regulate growth and flowering and substitute for diverse environmental stimuli,ʺ PNAS 103 : 6398-6403 (2006); Trankner, C. et al., "Over-expression of an FT-homologous gene of apple induces early flowering in annual and perennial plants," Planta 232 : 1309-1324 (2010); and Xiang, L. et al., ʺFunctional analysis of Flowering Locus T orthologs from spring orchid (Cymbidium goeringii Rchb. f.) that regulates the vegetative to reproductive transition,ʺ Plant Cell & Biochem 58 : 98-105 (2012), complete the contents of which are incorporated into this document by reference. However, previous studies with the expression of FT transgenes have used constitutive or tissue-specific promoters that have produced either very pronounced phenotypes, non-cellular autonomous (systemic) phenotypes, or autonomous leaf-specific phenotypes with earlier flowering or emergence compared to controls and termination more early stages of development. Given these facts, ectopic expression has generally not been seen as a viable approach to increase plant yield by inducing flower production or altering flowering time.

[37] Without wishing to be bound by theory, it is believed that an early florigenic signal (e.g., a short day for soybean and other KD plants) may initiate an early transition from vegetative to generative state in plants, but may also lead to termination of part of the primary meristem. At the same time, the return of these plants to non-inductive growth conditions (for example, a long day for KD plants) after the initial KD signal can preserve the remaining reserves of the plant meristem, which will make it possible to continue the vegetative growth of the plant. Thus, more productive flowers, pods and/or seeds per node (and/or plant) can develop during the extended generative phase. By inducing early flowering it is also possible to create more overlap between generative development and vegetative growth of the plant, which can further encourage or coincide with longer generative and/or flowering periods. In the context of this document, the expression "duration of the generative period" refers to the time from the initiation of flowering to the end of seed/bean development and/or filling, while the "duration of flowering" or "duration of the flowering period" refers to the time from the appearance of the first open flower to the withering of the last open flower. By returning to non-inductive growth conditions after early flowering induction, greater resources will be available and devoted to increasing the number of previously synchronized and productive (i.e., non-empty) flowers, pods, and/or seeds per plant, as opposed to normal flower development in plants. a short day, in which a decrease in plant resources can occur later due to the termination of meristematic growth and maturation of the plant.

[38] However, as noted above, a flowering induction signal (eg, early short day conditions) can also lead to early plant termination in addition to early flowering. Therefore, it has been suggested that optimal dose and timing of the flowering induction signal may be needed to maximize yield by striking a balance between (i) early transition from vegetative to generative stage and/or timing of flowering with an early flowering induction signal (resulting in potential yield gains). at each plant node) and (ii) early termination of growth (resulting in smaller plants with fewer internodes, less branching, and fewer nodes and/or flowers per plant). Smaller doses of the flowering induction signal may be sufficient to induce flowering while reducing or minimizing the effect of early plant termination, resulting in larger plants with an increased number of flowers, pods and/or seeds per node (and/or plant). On the other hand, higher doses of the flowering induction signal can lead to early termination of the plant (in addition to early flowering) with smaller plants with relatively few flowers, pods and/or seeds per plant due to smaller plant size with fewer internodes and/or branches. per plant, despite the possible presence of more flowers, pods and/or seeds per node (and/or plant) compared to wild-type plants or control plants under normal growth conditions. As stated above, these effects of ectopic FT expression may also include an increased number of pods, pods, fruits, nuts, tubers, etc. per node (and/or plant) depending on the particular plant species.

[39] The "short daylight" induction phenotype mentioned above for soybean was used to screen for genes having altered expression in these plants by transcription profiling, which resulted in the identification of an endogenous FT gene, Gm.FT2a , having increased expression in response to short-day induction conditions. Accordingly, it has been suggested that expression of a florigenic FT transgene such as Gm.FT2a can be used as a flowering induction signal to induce early flowering and increase the number of flowers, pods and/or seeds per node (and/or plant) by compared to a wild-type plant or a control plant that does not contain the FT transgene. According to embodiments of the present invention, appropriate control of the timing, location, and dose of florigenic FT expression during the vegetative stages of development can be used to induce flowering and produce plants having a higher number of flowers, pods, and/or seeds per node compared to a wild-type plant. or a control plant that does not contain the FT transgene. Instead of light conditions, FT rICD can be expressed at a low level in the vegetative meristem to provide an early flowering induction signal. Accordingly, a promoter from the Erecta gene ( pErecta or pEr ), characterized by lower meristematic expression during vegetative stages of development, was selected for initial study with the Gm.FT2a transgene. However, given that previous studies have shown that constitutive expression of FT results in plants having a pronounced early termination phenotype, and additionally that the site of action for peripherally produced and translocated from leaves is in the meristem, it was possible that direct meristematic expression of FT could result in even stronger and more pronounced phenotypes (and/or nonviable plants) compared to constitutive FT expression.

[40] The effect of Gm.FT2a overexpression with the pErecta promoter was immediately apparent in the R ₀ generation of transformed soybean plants, which were characterized by early flowering, reduced seed yield (e.g., only about 8 seeds/plant), and very early termination, suggesting that the balance between flowering induction and flowering repression/vegetative growth was strongly biased towards flowering and early death. However, enough R1 seeds were collected from these plants to allow for additional experiments. It has been hypothesized that growing R ₁ generation soybean seeds under long-day photoperiod (bloom repressive) conditions in a greenhouse may retard the development of the early flowering and death phenotypes seen in R ₀ plants. Given the theoretical dose response, it was further suggested that the segregation of homozygous, hemizygous, and FT2a null soybean plants could be examined together in the greenhouse to evaluate the dose response resulting from FT overexpression. In these experiments (described further below), segregated plants were observed to have different phenotypes: null plants were similar to wild-type plants in terms of plant architecture and number of pods per node (and per plant), while homozygous plants died early and had a strong dwarf phenotype (although possibly with an increased number of pods per node). However, the hemizygous plants were larger and more similar to null plants or wild type plants, but exhibited an enhanced flowering phenotype with an increased number of pods per node (and/or per plant). These findings indicate that expression of the FT transgene in the vegetative stage and/or in the meristem can be used to produce a high yielding plant (similar to the use of rICD conditions) and that the effect of FT expression may be dose-dependent since soybean plants hemizygous for the FT2a transgene are controlled a weak meristematic promoter showed a high yielding phenotype with an increased number of pods per node without the more pronounced early death and low plant height phenotypes seen in FT2a homozygous plants when grown under long day (vegetative) conditions.

[41] Accordingly, expression of the FT transgene at the vegetative stage at an appropriate dose level can be used to induce early flowering and produce plants having an increased number of flowers, pods, pods, pods, fruits, nuts, tubers, and/or seeds per node compared to with a wild-type plant or a control plant that does not contain the FT transgene. An appropriate dose level of FT can be provided by the promoter chosen to drive the expression of the FT transgene. Weaker or lower levels of FT transgene expression in the vegetative meristem can be used to signal the induction of early flowering while maintaining or prolonging the duration of generative and/or flowering development and not leading to too early termination of plant development. Again, a promoter from the Erecta gene ( pErecta or pEr ), characterized by lower meristematic expression during the vegetative stages of development, was selected for initial study with the Gm.FT2a transgene in soybeans. As further described below, other promoters with similar profiles and expression levels in the vegetative meristem had similar effects in soybean plants, such as early flowering and/or increased number of pods per node. Separately or in addition to promoter selection, different FT transgenes from soybean or other species can be used to influence the expression level of the transgene, which can reduce the “dose” of the early FT signal delivered to the vegetative meristem, depending on the level of activity of the FT transgenic protein in the plant cell. . Indeed, several FT transgenes from soybean and other plant species have been investigated and shown to have different effects on flowering and other generative traits when transgene expressed in soybean plants.

[42] Without being limited by theory, it has been further suggested that the increased number of pods per node in plants with the FT transgene may be the result, at least in part, of an increase in the number of inflorescence meristems and floral meristems induced from vegetative shoot apical and axillary meristems in each from affected nodes, which may result in more flowers and/or released flower clusters at those nodes. Such an increase in the number of floral meristems induced at each plant node in response to FT overexpression may occur through one or more mechanisms or pathways that may be independent of flowering time and/or duration of the generative stage. However, meristematic changes may be microscopic at first and therefore, when observed, do not produce "early flowering" at such a stage by simple visual inspection, although generative changes in the meristem may already be beginning to take place. Early vegetative expression of FT may result in earlier than normal formation and development of generative meristems at one or more nodes of the transgenic plant. These generative meristems can then provide or cause the formation of more flower clusters, followed by the appearance of flowers on them at each node. Without being limited by theory, an additional suggestion has been made that later expression of FT during the generative stages may suppress the development of flowers at each node. Thus, the later developing flowers within the respective raceme may die and thus more plant resources can be directed to the earlier developing flowers and generative structures within the raceme to produce full-sized pods more efficiently. The early flowering induction signal can also cause a large proportion of the existing meristematic potential to become generative and go through the flower stage. Accordingly, increased timing of flower development can occur, producing more mature pods per plant node.

[43] However, as noted above, the flowering induction signal also leads to early termination of plant development in addition to early flowering. Although soybean plants expressing various FT transgenes with a meristematic vegetative stage promoter such as the pErecta promoter have an increased number of pods per node on the main stem of the plant, many of these plants still show reduced plant height and/or reduced branching, resulting in less the number of nodes per plant, main stem and/or branches. Thus, although the level of FT expression in a plant can be controlled by selecting a particular vegetative stage promoter to suppress out-of-development types and increase yield by slowing down early termination, transgenic FT-expressing soybean plants can still have a reduced number of flowering nodes per plant, which can reduce overall plant yield despite an increased number of nodes per existing node on the main stem.

[44] Therefore, this document proposes that despite the observed increase in the number of beans per node in soybean plants expressing FT at the vegetative stage, it may be necessary to further attenuate, regulate or limit the expression level of the FT transgene to mitigate these early termination phenotypes and thus achieving optimal yields. If an increased number of pods (or other botanical structures such as pods, pods, fruits, nuts, tubers, etc.) can be maintained per plant node while increasing the number of pods per plant, for example by avoiding or slowing down termination, then it will be possible to further optimize or improve the overall yield of the plant.

[45] According to embodiments of the present invention, it is proposed herein that plant yield can be increased or increased by attenuating or modifying the level and/or timing of expression of the FT transgene through suppression. As further described below, the amount or spatio-temporal profile of the transgenic FT can be reduced and/or adjusted by naturally occurring and/or engineered RNA molecules that target the transgenic FT for suppression. It has been suggested that although transgenic FT expression in the axillary and apical meristem may initiate their transfer to the floral meristem, continued FT expression, for example, during generative stages of development, may lead to early termination of the meristem and arrest of growth and branching of the whole plant. Although it is possible to select and use a vegetative stage promoter to express the FT transgene to attenuate the early termination phenotype and maintain or prolong vegetative growth and generative stage length of the plant, additional suppression of the FT transgene can further mitigate the early termination phenotype and improve or enhance growth, development, and generative stage duration. plants. Indeed, the present inventors have observed that suppression of the FT transgene in soybean can result in more normal plant height and branching with higher node numbers per plant compared to expression of the FT transgene alone (i.e., no suppression). Soybean plants with combined expression/suppression of the FT transgene will retain a higher number of beans per node with additional mitigation of the early termination phenotype seen only when the FT transgene is expressed.

[46] Suppression of the FT transgene can be achieved in a variety of ways. According to a first approach, the FT transgene of the first expression cassette can be suppressed by a second expression cassette encoding an RNA molecule that targets the FT transgene for suppression. The RNA molecule may be encoded by a transcriptionable DNA sequence operably linked to a promoter suitable for expression in plants, wherein the transcriptionable DNA sequence contains a targeting sequence that corresponds to at least a portion of the FT transgene and/or its complementary sequence. In a second approach, the FT transgene may encode a target site for an endogenous RNA molecule, wherein the target site is complementary to the endogenous RNA molecule such that the endogenous RNA molecule targets the FT transgene for suppression. An endogenous RNA molecule may naturally exist in a plant cell in which the FT transgene is expressed. According to this approach, suppression does not require a second expression cassette. Also, both of these approaches for suppression can be used together. For example, the FT transgene may be present in a first expression cassette and used in combination with a second expression cassette containing a DNA sequence suitable for transcription encoding an RNA molecule containing a targeting sequence that corresponds to at least a portion of the FT transgene (and/or its complementary sequence). ) (i.e., the first target site) so that the RNA molecule targets the FT transgene for suppression, and the FT transgene additionally contains a second target site for the endogenous RNA molecule that also targets the FT transgene for suppression. The first and second target sites may be the same or different in sequence and may be at the same or different locations in the FT transcript. As used herein, a first polynucleotide sequence or molecule "corresponds" to a second polynucleotide sequence or molecule if the first sequence or molecule is similar, identical and/or complementary to a second sequence or molecule, e.g., more than 30%, more than 40% more than 50%, more than 60%, more than 70%, more than 80%, more than 90%, more than 95% or 100% identical and/or complementary.

[47] In accordance with one aspect of the present invention, a recombinant DNA molecule, vector, or construct is provided, comprising at least two expression cassettes, including a first expression cassette and a second expression cassette, wherein the first expression cassette contains a polynucleotide sequence encoding an FT protein (t e. FT transgene) operably linked to a first promoter suitable for expression in plants, and the second expression cassette contains a DNA sequence suitable for transcription operably linked to a second promoter suitable for expression in plants, and the DNA sequence suitable for transcription contains a sequence that corresponds to at least part of the FT transgene. The transcribed DNA sequence may encode an RNA molecule containing a targeting sequence that is complementary to at least a portion of the pre-mRNA or mature mRNA encoded by the polynucleotide sequence encoding the FT protein (i.e., the FT transgene) of the first expression cassette, such that the function of the RNA molecule consists in the suppression of the FT transgene. The "targeting sequence" of an RNA molecule encoded by a transcriptionable DNA sequence may comprise all or part of the RNA molecule and is encoded by a transcriptionable DNA sequence that corresponds to at least a portion of the FT transgene and/or its complementary sequence. Thus, a DNA sequence suitable for transcription can encode an RNA molecule that contains a targeting sequence that is complementary to at least a portion of the mRNA transcript of the FT transgene. Depending on the specific FT transgene and/or the targeting sequence of the RNA molecule encoded by a DNA sequence suitable for transcription, the endogenous FT gene may also be suppressed by a second expression cassette in addition to the FT transgene, or the endogenous FT gene may be suppressed by a second expression cassette instead of the FT transgene. Many FT gene and protein sequences may be identical or similar to one or more native or endogenous FT genes in the transformed plant and thus serve as a basis for constructing RNA molecules and targeting sequences to suppress these native and endogenous genes. When a DNA sequence suitable for transcription encodes an RNA molecule that targets and represses an FT transgene, an endogenous FT gene, or both, the overall level of expression and activity of the genome and FT transgenes can be controlled, restricted, or reduced in one or more tissues of the transgenic plant. In one aspect, a nucleic acid molecule containing a targeting sequence is capable of hybridizing to a complementary nucleic acid sequence ( eg, target site) to form a double-stranded nucleic acid ( eg , dsRNA). In one aspect, hybridization of a targeting sequence of a first nucleic acid molecule (eg, a suppressor RNA molecule) with a target site sequence of a second nucleic acid molecule (eg, an mRNA transcript of an FT transgene) may result in suppression of the second nucleic acid molecule. For example, if the polynucleotide sequence of the FT transgene is native to the plant in which it is expressed (or closely related), then the RNA molecule can further target the corresponding native FT gene for suppression, especially if the RNA molecule targets the coding sequence (exon) of the FT transgene. . In such cases, the combined suppression of native and transgenic FT genes by an RNA molecule encoded by a DNA sequence suitable for transcription can further reduce the dose of the FT protein in the respective plant tissues. However, even when the native FT gene is expressed transgenicly, non-protein-coding sequences such as 5' UTR, 3' UTR, leader and/or intron sequences can be varied without affecting the sequence of the encoded FT protein. In the context of this document, the "coding polynucleotide sequence" or "polynucleotide sequence" of a transgene may contain not only protein (or exon) coding sequences, but also other transcriptionable sequences associated with the coding sequence of the transgene, which may form part of the encoded pre-mRNA sequence. or mature mRNA, such as 5' UTR, 3' UTR, leader and/or intron sequences. Thus, in the context of this document, the expressions "coding polynucleotide sequence" of the FT transgene and "polynucleotide sequence" encoding the FT protein can be used interchangeably.

[48] According to embodiments of the present invention, the first expression cassette and the second expression cassette may be in the same recombinant DNA molecule, vector, or construct, or the first expression cassette and the second expression cassette may be in separate recombinant DNA molecules, vectors, or constructs. Thus, in accordance with some embodiments of the invention, two recombinant DNA molecules, two vectors, or two constructs may be provided comprising a first recombinant DNA molecule, vector or construct and a second recombinant DNA molecule, vector or construct, wherein the first recombinant DNA molecule, the vector or construct contains a first expression cassette containing a polynucleotide sequence encoding an FT protein (i.e., an FT transgene) operably linked to a first promoter suitable for expression in plants, and the second recombinant DNA molecule, vector or construct contains a second expression cassette containing a DNA sequence suitable for transcription operably linked to a second promoter suitable for expression in plants, wherein the DNA sequence suitable for transcription corresponds to at least a portion of the FT transgene and/or its complementary sequence. The transcribed DNA sequence may encode an RNA molecule containing a targeting sequence that is complementary to at least a portion of the pre-mRNA or mature mRNA encoded by the FT transgene of the first expression cassette, such that the function of the RNA molecule is to suppress the FT transgene.

[49] Proposed herein, a recombinant DNA molecule, vector or construct containing the FT transgene can be used in plant transformation to create a transgenic plant containing the FT transgene. In accordance with some embodiments of the invention, the FT transgene may be present in a first expression cassette and used in combination with a second expression cassette containing a DNA sequence suitable for transcription encoding an RNA molecule containing a targeting sequence that corresponds to at least a portion of the FT transgene and/or a sequence complementary to it, wherein the first and second expression cassettes are transformed into a plant as one or separate transformation events. In accordance with some embodiments of the invention, the FT transgene transformed into a plant may contain a target site for an endogenous RNA molecule that can target the FT transgene and initiate its suppression.

[50] The coding polynucleotide sequence of the FT transgene may include Gm.FT2a (SEQ ID NO: 1) or any polynucleotide sequence encoding the Gm.FT2a protein (SEQ ID NO: 2). The coding polynucleotide sequence of the FT transgene may also correspond to other FT genes in soy or other plants. For example, other soybean coding polynucleotide sequences that can be used as an FT transgene according to the present embodiments of the invention include: Gm.FT5a (SEQ ID NO: 3) or a polynucleotide sequence encoding a Gm.FT5a protein (SEQ ID NO: 4), or Gm.FT2b (SEQ ID NO: 5) or a polynucleotide sequence encoding a Gm.FT2b protein (SEQ ID NO: 6). In addition, examples of coding polynucleotide sequences from other plant species that can be used include: Zm.ZCN8 (SEQ ID NO: 7) from maize or a polynucleotide sequence encoding a Zm.ZCN8 protein (SEQ ID NO: 8), Nt.FT -like or Nt.FT4 (SEQ ID NO: 9) from tobacco or a polynucleotide sequence encoding an Nt.FT-like protein or Nt.FT4 (SEQ ID NO: 10), Le.FT or SFT protein (SEQ ID NO: 11 ) from tomato or a polynucleotide sequence encoding a Le.FT or SFT protein (SEQ ID NO: 12), At.FT (SEQ ID NO: 13) from Arabidopsis or a polynucleotide sequence encoding an At.FT protein (SEQ ID NO: 14) , At.TSF (SEQ ID NO: 15) from Arabidopsis or a polynucleotide sequence encoding protein At.TSF (SEQ ID NO: 16), Os.HD3a (SEQ ID NO: 17) from rice or a polynucleotide sequence encoding Os protein. HD3a (SEQ ID NO: 18), or Pt.FT (SEQ ID NO: 19) from Populus trichocarpa , or a polynucleotide sequence encoding a Pt.F protein T (SEQ ID NO: 20). Additional examples of coding polynucleotide sequences for FT transgenes and proteins that may also be used include the following: Gm.FT5b (SEQ ID NO: 21) from soy or any polynucleotide sequence encoding a Gm.FT5b protein (SEQ ID NO: 22); Gh.FT1 (SEQ ID NO: 23) from cotton or any polynucleotide sequence encoding a Gh.FT1 protein (SEQ ID NO: 24); Bn.FTA2a (SEQ ID NO: 25) from canola, or any polynucleotide sequence encoding a Bn.FTA2a protein (SEQ ID NO: 26); Ta.FT3B1 (SEQ ID NO: 27) from wheat, or any polynucleotide sequence encoding the Ta.FT3B1 protein (SEQ ID NO: 28); or Ps.FTa1 (SEQ ID NO: 29) from pea, or any polynucleotide sequence encoding a Ps.FTa1 protein (SEQ ID NO: 30). Coding polynucleotide sequences for FT transgenes encoding additional FT proteins from other species having known amino acid sequences may also be used in accordance with embodiments of the present invention, which may, for example, include the following: Md.FT1 and Md.FT2 from apple ( Malus domestica ); Hv.FT2 and Hv.FT3 from barley ( Hordeum vulgare ); Cs.FTL3 from chrysanthemum; Ls.FT from lettuce ( Lactuca sativa ); Pn.FT1 and Pn.FT2 from pyramidal poplar ( Populus nigra ); Pa.FT from maple-leaved sycamore ( Platanus acerifolia ); Dl.FT1 from longan ( Dimocarpus longan ); Ps.FTa1, Ps.FTa2, Ps.FTb1, Ps.FTb2 and Ps.FTc from pea ( Pisum sativum ); Ac.FT from pineapple ( Ananas comosus ); Cm-FTL1 and Cm-FTL2 from pumpkin ( Cucurbita maxima ); Ro.FT from rose; Cg.FT from spring orchid ( Cymbidium ); Fv.FT1 from strawberry ( Fragaria vesca ); Bv.FT2 from sugar beet ( Beta vulgaris ); Ha.FT4 from sunflower ( Helianthus annuus ); and Ta.FT or TaFT1 from wheat ( Triticum aestivum ), and sequences that are at least 60% identical, at least 65% identical, at least 70% identical, at least 75% identical, at least 80% identical, at least 85% identical, or at least 90%, at least 91%, at least 92%, at least 93%, at least 94% , at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identical with one or more such known polynucleotide and/or protein sequences. See, for example, Wickland, DP et al., "The Flowering Locus T/Terminal Flower 1 Gene Family: Functional Evolution and Molecular Mechanisms", Molecular Plant 8 : 983-997 (2015), the contents of which are incorporated herein by reference.

[51] Unless otherwise indicated, nucleic acid or polynucleotide sequences described herein are shown (left to right) in the 5' to 3' direction, and amino acid or protein sequences are shown (left to right) in the N-terminal to C direction. -end. Additional known or later discovered FT genes and proteins from these or other species may also be used in accordance with embodiments of the present invention. These FT genes may be known or reconstructed from their nucleotide and/or protein sequences, which may be determined by visual inspection or by using computer search and identification tools or computer software (and databases) based on an algorithm for comparison with known sequences, structural FT domains, etc. and according to any known sequence alignment method such as BLAST, FASTA, etc.

[52] In accordance with embodiments of the present invention, the FT transgene of a recombinant DNA molecule, vector, or construct may contain a polynucleotide sequence that, when optimally aligned) is at least 60% identical, at least 65% identical, at least 70% identical, at least 75% identical, at least 80% identical, at least 85% identical, or at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% identical to one or more of the FT polynucleotide coding sequences listed above (e.g., SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, or 29), or any other known coding sequence for florigenic FT. The percent sequence identity among the polynucleotide sequences of the above coding sequences for the FT genes is shown in FIG. 1A. In each cell in the table in Fig. 1A shows the percent identity for the FT gene in the respective row (query sequence) compared to the FT gene in the respective column (sequence of interest) divided by the total length of the query sequence, and the number in brackets represents the total number of identical bases between the query and the sequence of interest. As illustrated in this figure, the percent identity among polynucleotide sequences for these FT gene samples ranges from about 60% to about 90% identity. Thus, a polynucleotide sequence that falls within one or more of these sequence identity ranges, or has a higher sequence identity, can be used in accordance with embodiments of the present invention to induce flowering, increase yield, and/or change one or more plant generative traits. Similar coding polynucleotide sequences for FT can be designed or selected based on known FT protein sequences, conserved amino acid residues and domains, degeneracy of the genetic code, and any known codon optimization for the particular plant species to be transformed.

[53] According to embodiments of the present invention, the FT transgene may comprise a polynucleotide sequence encoding an amino acid or protein sequence that (when optimally aligned) is at least 60% identical, at least 65% identical, at least 70% identical, at least 75% identical, at least 80% identical, at least 85% identical, or at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% identical to one or more of the FT protein or amino acid coding sequences listed above (e.g., SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, or 30) , or any other known florigenic FT protein sequence or functional fragment thereof. Such a "functional fragment" is defined as a protein having a polypeptide sequence identical or very similar to the full-length FT protein, but lacking one or more amino acid residues, portions, protein domains, etc. of the full-length FT protein, provided that the fragment remains active in that it results in one or more phenotypic effects or changes similar to a full length protein when expressed transgenic in a plant. The percent sequence identity among the above full-length FT proteins is shown in FIG. 1b. The percentage is calculated as described above with reference to FIG. 1A based on the number of identical amino acid residues (in brackets) between the queried and considered FT protein sequences. The multiple alignment of these FT proteins is also illustrated in FIG. 1C. As can be seen from these figures, the percent identity among the protein sequences for these FT genes ranges from about 60% to about 90% identity. Thus, a polynucleotide sequence encoding an amino acid or protein sequence that falls within one or more of these sequence identity ranges or has a higher sequence identity can be used in accordance with embodiments of the present invention to induce flowering, increase yield, and/or change one or more generative traits of the plant. These FT protein sequences encoded by the polynucleotide sequence of this invention may be designed or selected based on known FT protein sequences and their conservative amino acid residues and domains.

[54] As described below, an FT transgene containing one of the above coding sequences may further comprise one or more expression and/or regulatory elements such as enhancer(s), promoter(s), leader(s) (s), intron(s), etc., and the FT transgene may contain a genomic sequence encoding an FT protein or an amino acid sequence, or a fragment or part thereof.

[55] As used herein, "sequence identity" or "percent identity" refers to the degree to which two optimally aligned DNA or protein sequences are identical. Various algorithms and programs for pairwise or multiple sequence alignment are known in the art, such as ClustalW, etc., which can be used to compare sequence identity or similarity between two or more sequences, e.g. between two or more genes or protein sequences. FT, or between an FT gene (nucleotide) or protein sequence and another nucleotide or protein sequence. For example, the percent identity of one sequence (requested) with another sequence (considered) can be calculated as described above with reference to FIG. 1A and 1B (ie, for optimal sequence alignment, divide the number of identical bases or residues by the total number of bases or residues for the requested sequence and multiply by 100%). Although other alignment and comparison methods are known in the art, alignment and percent identity between two sequences (including percent identity in the range described above) can be determined using the ClustalW algorithm, see, for example, Chenna R. et al., ʺMultiple sequence alignment with the Clustal series of programs,ʺ Nucleic Acids Research 31 : 3497-3500 (2003); Thompson JD et al., 'Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice,' Nucleic Acids Research 22 : 4673-4680 (1994); and Larkin MA et al., "Clustal W and Clustal X version 2.0," Bioinformatics 23 : 2947-48 (2007), the entire contents of which are incorporated herein by reference. For the purposes of this invention, when two sequences are optimally aligned (with gap tolerance in the alignment), the "percent identity" for the requested sequence is calculated as described above with reference to FIG. 1A and 1B - i.e. percent identity = (number of identical positions between the query and the sequence in question / total number of positions in the query sequence) x 100%, where each sequence consists of a number of positions (nucleotide bases or amino acid residues). Two optimally aligned sequences can also be described as having a certain percentage of identity. Optionally, percent identity can be described with reference to a specific comparison window (eg, alignment window) between two sequences, in which case the number of identical positions in the comparison window is divided by the nucleotide length of the comparison window and multiplied by 100%. The alignment window can be defined as the area of identity, similarity, or overlap between two sequences.

[56] The recombinant polynucleotide or protein molecule or construct or vector can be isolated. As used herein, the term "isolated" refers to at least partial separation of a molecule from other molecules normally associated with it in its natural state. In one embodiment of the invention, the term "isolated" refers to a DNA molecule that is separated from the nucleic acids that normally flank the DNA molecule in its natural state. For example, a DNA molecule encoding a protein that is naturally present in a bacterium is considered an isolated DNA molecule if it is not found in the DNA of a bacterium in which a DNA molecule encoding a protein can be found in a natural state. Thus, a DNA molecule fused or operably linked to one or more DNA molecules to which it would not naturally be associated, such as through recombinant DNA technology or plant transformation, is considered isolated herein. Such molecules are considered isolated even when they are integrated into the host cell's chromosome or are in nucleic acid solution with other DNA molecules.

[57] An FT protein sequence encoded by a polynucleotide sequence or a transgene of the present invention may also be designed or selected to contain one or more amino acid substitutions that are chemically and/or structurally conservative (e.g., substitution of one amino acid for another having similar chemical or physical properties such as hydrophobicity, polarity, charge, steric effect, acidic/basic chemistry, similar side chain groups such as hydroxyl, sulfhydryl, amino, etc.) in order to avoid or minimize structural changes in the protein which may affect its function. For example, valine is often a conservative substitute for alanine, while threonine may be a conservative substitute for serine. Additional examples of conservative amino acid substitutions in proteins include: valine/leucine, valine/isoleucine, phenylalanine/tyrosine, lysine/arginine, aspartic acid/glutamic acid, and asparagine/glutamine. An FT protein sequence encoded by a polynucleotide sequence or a transgene of the invention may also include proteins that differ by one or more amino acids from a known FT protein sequence or similar sequence as a result of deletion(s) and/or insertion(s) comprising one or more amino acids.

[58] It is possible to identify different FT genes and proteins from different plant species and consider them as FT homologs or orthologs for use in the present invention if they have a similar nucleic acid and/or protein sequence and contain conserved amino acids and/or structural domains with at least one known FT gene or protein. In the context of this document, the term "homolog" in relation to a FT gene or protein is intended to include any homologues, analogs, orthologues, paralogs, etc. of the FT gene or protein, and the term "homologous" in relation to polynucleotide or protein sequences means similar or identical sequences, including synthetic, artificial or engineered polynucleotide or protein sequences. Such an FT homologue can also be defined as having the same or similar biological function as known FT genes (eg, with a similar effect on flowering and/or other generative or yield-related traits or phenotypes when ectopically expressed in the plant).

[59] Sequence analysis and alignment of FT protein sequences from different plant species further identified a number of conserved amino acid residues and at least one conserved structural domain. When applied to various aligned FT protein sequences (see, for example, FIGS. 1B and 1C) a protein domain detection tool using the Pfam database (for example, Pfam 26.0 released November 2011 or later), there was these FT proteins were found to contain at least a portion of the putative phosphatidylethanolamine-binding protein (PEBP) domain (Pfam domain name: PBP_N; accession number: PF01161). See, for example, Banfield, MJ et al., ʺThe structure of Antirrhinum centroradialis protein (CEN) suggests a role as a kinase inhibitor,ʺ Journal of Mol Biol., 297 (5): 1159-1170 (2000), the full contents of which incorporated into this document by reference. This PEBP domain was found to correspond, for example, to amino acids 28-162 of the full length Gm.FT2a protein (see Table 5 below). Thus, FT proteins included in embodiments of the present invention may include those identified or characterized as having or containing at least a PEBP domain (accession number: PF01161) according to the Pfam assay. Accordingly, the invention may further include polynucleotide sequences encoding an FT protein containing at least a PEBP domain. As is known in the art, the Pfam database is a large collection of multiple sequence alignments and hidden Markov models covering many common protein families and contains information about the various protein families and their domain structures. By determining putative Pfam structural domains for a given protein sequence, the classification and function of a protein can be elucidated or determined. See, for example, Finn, RD et al., "The Pfam protein families database," Nucleic Acids Research (Database Issue), 42 :D222-D230 (2014), the entire contents of which are incorporated herein by reference.

[60] Embodiments of the present invention may further include polynucleotide sequences encoding inductive or florigenic FT proteins. An FT protein encoded by a polynucleotide sequence may be "inductive" or "florigenic" if the FT protein, when ectopically expressed in the plant, is capable of leading to earlier flowering and/or increased fruiting in the context of the number of flowers, pods, pods, pods, fruits, nuts, tubers and/or seeds on one or more plant nodes. Without being limited by theory, it has been further suggested that such an increase in fruitfulness in the context of the number of flowers, pods, pods, pods, fruits, nuts, tubers and/or seeds per one or more plant nodes may result from an increase in the number of meristems at those nodes, which pass from vegetative to generative stage and produce flowers. This increased fecundity at each node due to ectopic expression of "florigenic" FT may be associated with increased timing of release and floral development of early brushes and lateral meristems at each node. Although the function of the "florigenic" FT protein may be to induce early flowering when ectopically expressed in the plant, the transgenic expressed "florigenic" FT protein can increase the number of flowers, pods, pods, pods, fruits, nuts, tubers, and/or seeds per node ( nodes) of the plant through one or more pathways or mechanisms, independently of or in addition to any florigenic effects associated with flowering time and/or duration of the generative stage.

[61] Florigenic FT-like genes from various plant species are generally highly conserved. However, many proteins in the PEBP family have amino acid sequences that are substantially similar to the florigenic FT proteins, but do not appear to be florigen. For example, Terminal Flower (TFL) genes from various plant species have similar protein sequences to florigenic FT genes, but actually slow down flowering. Recent work has identified specific amino acid residues that are not generally shared between florigenic FT proteins and other PEBP proteins such as TFL, and it has been shown that substitutions at many of these positions convert florigenic FT proteins into flowering repressor proteins. See, for example, Ho and Weigel, Plant Cell 26: 552-564 (2014); Danilevskaya et al., Plant Physiology 146 (1): 250-264 (2008); Harig et al., Plant Journal 72 : 908-921 (2012); Hsu et al., Plant Cell 18 : 1846-1861 (2006); Kojima et al., Plant Cell Physiology 43 (10): 1096-1105 (2002); Kong et al., Plant Physiology 154 : 1220-1231 (2010); Molinero-Rosales et al., Planta 218 : 427-434 (2004); Zhai et al., PLoS ONE , 9 (2): e89030 (2014), and Wickland DP et al. (2015), supra , the entire contents of which are incorporated herein by reference. Thus, amino acid residues can serve as indicators to further define and discriminate the florigenic FT proteins of the present invention.

[62] According to embodiments of the present invention, an "inductive" or "florigenic" FT protein can be further defined or characterized as containing one or more of the following amino acid residues (amino acid positions refer to the corresponding or optimally aligned positions of the full-length Arabidopsis FT protein, SEQ ID NO: 14): proline at amino acid position 21 (P21); arginine or lysine at amino acid position 44 (R44 or K44); glycine at amino acid position 57 (G57); glutamic acid or aspartic acid at amino acid position 59 (E59 or D59); tyrosine at amino acid position 85 (Y85); leucine at amino acid position 128 (L128); glycine at amino acid position 129 (G129); threonine at amino acid position 132 (T132); alanine at amino acid position 135 (A135); tryptophan at amino acid position 138 (W138); glutamic acid or aspartic acid at amino acid position 146 (E146 or D146); and/or cysteine at amino acid position 164 (C164). The corresponding amino acid positions of other FT proteins can be determined by alignment with the Arabidopsis FT sequence (see, for example, Fig. 1C). One skilled in the art will be able to determine the corresponding amino acid positions of other FT proteins based on the sequence alignment. Several of these key residues are within the outer loop domain of FT-like proteins defined by amino acids 128-145 of the full-length Arabidopsis FT sequence (SEQ ID NO: 14) and the corresponding sequences of other FT proteins (see, for example, Fig. 1C). Thus, the polynucleotides of the invention may encode FT florigenic proteins having one or more of these conserved amino acid residues.

[63] The florigenic FT proteins of the present invention may also contain one or more other amino acids at one or more of the residue positions defined above. For example, in the case of the above amino acid positions of the Arabidopsis FT protein (At.FT) sequence (SEQ ID NO: 14), the florigen FT protein may alternatively contain one or more of the following amino acids: alanine (instead of proline) at positions, the corresponding position 21 of the At.FT protein sequence (P21A), or possibly other small non-polar residues such as glycine or valine, at that position; histidine (instead of lysine or arginine) at the amino acid position corresponding to position 44 of the At.FT protein sequence, or possibly other polar amino acids at that position; alanine or cysteine (instead of glycine) at the amino acid position corresponding to position 57 of the At.FT protein sequence, or possibly other small non-polar residues such as proline or valine at that position; asparagine or serine (instead of glutamic acid or aspartic acid) at the amino acid position corresponding to position 59 of the At.FT protein sequence, or possibly other small polar residues such as glutamine, cysteine or threonine at that position; different polar and non-polar uncharged residues (other than leucine) at the amino acid position corresponding to position 85 of the At.FT protein sequence; a non-polar or hydrophobic uncharged residue (other than leucine) such as isoleucine, valine, or methionine at the amino acid position corresponding to position 128 of the At.FT protein sequence; various smaller non-polar and uncharged residues (other than glycine) such as alanine, valine, leucine, isoleucine, methionine, etc., at the amino acid position corresponding to position 129 of the At.FT protein sequence, although some charged and polar remnants; a polar uncharged residue (other than threonine) at the amino acid position corresponding to position 132 of the At.FT protein sequence; different amino acids other than proline, such as threonine, at the amino acid position corresponding to position 135 of the At.FT protein sequence; various other bulky non-polar or hydrophobic amino acids (instead of tryptophan), such as methionine or phenylalanine, at the amino acid position corresponding to position 138 of the At.FT protein sequence; various other polar or non-positively charged amino acids, such as asparagine or serine, at the amino acid position corresponding to position 146 of the At.FT protein sequence; and/or various other polar or non-polar amino acids (instead of cysteine) such as isoleucine at the amino acid position corresponding to position 164 of the At.FT protein sequence. One skilled in the art will be able to determine the appropriate amino acid positions and substitutions of FT proteins based on their sequence alignment with the Arabidopsis FT protein sequence. In addition, it is also contemplated that other chemically conservative amino acid substitutions acceptable from the point of view of a person skilled in the field of protein biochemistry may fall into the scope of the FT florigenic proteins. Accordingly, the polynucleotides of the present invention may further encode FT florigenic proteins having one or more conservative amino acid substitutions. Indeed, the florigenic FT proteins encoded by the polynucleotides of this invention include native sequences and artificial sequences containing one or more conservative amino acid substitutions, as well as functional fragments thereof.

[64] Florigenic FT proteins of the present invention can also be defined as excluding (i.e., not containing) one or more amino acid substitutions that may be characteristic of or associated with TFL or other non-florigenic or anti-florigenic proteins. For example, in the case of amino acid positions of the Arabidopsis FT protein sequence (SEQ ID NO: 14), one or more of the following amino acids may be omitted from the florigenic FT protein (i.e., at the corresponding or optimally aligned positions of the florigenic FT protein): phenylalanine or serine at the position corresponding to position 21 of the At.FT protein sequence (for example, instead of proline or alanine); phenylalanine at the position corresponding to position 44 of the At.FT protein sequence (for example, instead of arginine or lysine); histidine, glutamic acid, or aspartic acid at position corresponding to position 57 of the At.FT protein sequence (eg, instead of glycine); glycine or alanine at position corresponding to position 59 of the At.FT protein sequence (eg, instead of glutamic acid or aspartic acid); histidine at the position corresponding to position 85 of the At.FT protein sequence (for example, instead of tyrosine); lysine, arginine, alanine or methionine at the position corresponding to position 109 of the At.FT protein sequence; lysine or arginine at the position corresponding to position 128 of the At.FT protein sequence (for example, instead of leucine); glutamine or asparagine at the position corresponding to position 129 of the At.FT protein sequence (eg, instead of glycine); valine or cysteine at the position corresponding to position 132 of the At.FT protein sequence (for example, instead of threonine); lysine, arginine, or alanine at position corresponding to position 134 of the At.FT protein sequence (eg, instead of tyrosine); proline at the position corresponding to position 135 of the At.FT protein sequence (for example, instead of alanine or threonine); serine, aspartic acid, glutamic acid, alanine, lysine, or arginine at the position corresponding to position 138 of the At.FT protein sequence (for example, instead of tryptophan or methionine); lysine or arginine at the position corresponding to position 140 of the At.FT protein sequence; lysine or arginine at the position corresponding to position 146 of the At.FT protein sequence (eg, instead of acidic or uncharged polar residues); lysine or arginine at the position corresponding to position 152 of the At.FT protein sequence; and/or alanine at the position corresponding to position 164 of the At.FT protein sequence (eg instead of cysteine or isoleucine). One skilled in the art will be able to determine the appropriate amino acid positions and substitutions for other FT proteins based on their sequence alignment. Accordingly, embodiments of the present invention may exclude polynucleotides that encode FT-like proteins having one or more of the above amino acid substitutions associated with TFL or other antiflorigen. However, one or more of these amino acid substitutions may be allowed in the FT protein, as long as it retains florigenic activity.

[65] The florigenic FT protein of the present invention can also be defined as being similar to a known FT protein in addition to having one or more of the above characteristic or conserved amino acid residues. For example, a florigenic protein can be defined as having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% , at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity with a sequence selected from the group consisting of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 and 30, or its a functional fragment, in addition to one or more of the following characteristic residues: a tyrosine or other uncharged polar or non-polar residue (for example, alanine, tryptophan, methionine, leucine, threonine, cysteine, serine, or asparagine) at the amino acid position corresponding to position 85 of the protein sequence At.FT; a leucine or other non-polar or hydrophobic residue (eg isoleucine, valine or methionine) at the amino acid position corresponding to position 128 of the At.FT protein sequence; and/or tryptophan or another large non-polar or hydrophobic residue (eg, methionine or phenylalanine) at the amino acid position corresponding to position 138 of the At.FT protein sequence. Such a florigenic FT protein can be further defined as having additional characteristic amino acid residues such as one or more of the following: glycine or another small non-polar and uncharged residue (e.g. alanine, valine, leucine, isoleucine, or methionine) at the amino acid position corresponding to position 129 At.FT protein sequences; and/or threonine at the amino acid position corresponding to position 132 of the At.FT protein sequence.

[66] The florigenic FT protein of the present invention can also be defined as having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85% at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% identity with a sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 and 30, or a functional fragment thereof, but lacking (i.e., excluding) one or more non-florigenic or anti-florigenic residues, such as one or more of the following: a histidine at the amino acid position corresponding to position 85 of the At.FT protein sequence; lysine or arginine at the amino acid position corresponding to position 128 of the At.FT protein sequence; and/or serine, aspartic acid, glutamic acid, lysine or arginine at the amino acid position corresponding to position 138 of the At.FT protein sequence. Such a florigenic FT protein can be further defined as having no (ie, exclusive) one or more additional residues, such as one or more of the following: glutamine or asparagine at the amino acid position corresponding to position 129 of the At.FT protein sequence; and/or valine or cysteine at the amino acid position corresponding to position 132 of the At.FT protein sequence.

[67] According to embodiments of the present invention, there is provided a recombinant DNA molecule, vector, or construct comprising a polynucleotide sequence encoding an FT protein that is operably linked to one or more promoters and/or other regulatory elements whose function in a plant cell is to regulate or shifting the timing and/or location of FT expression upon transformation into a plant. In accordance with some embodiments of the invention, the FT transgene may be located in a first expression cassette and used in conjunction with a second expression cassette containing a DNA sequence suitable for transcription that corresponds to at least a portion of the FT transgene and/or its complementary sequence, and encodes an RNA molecule , which targets the FT transgene for suppression. In accordance with some embodiments of the invention, the FT transgene may contain a target site for an endogenous RNA molecule that can target the FT transgene and initiate its suppression.

[68] As commonly understood in the art, the term "promoter" can generally refer to a DNA sequence that contains an RNA polymerase binding site, a transcription initiation site, and/or a TATA box and causes, initiates, regulates, maintains, and/ or stimulates transcription and expression of an associated transcribable polynucleotide sequence and/or gene (or transgene). A promoter can be synthetically created, engineered, varied, and/or derived from a known or natural promoter sequence, or another promoter sequence (eg, as provided herein). The promoter may also include a chimeric promoter containing a combination of two or more heterologous sequences. Thus, the promoter of this invention may include variants of promoter sequences that are similar in composition to, but not identical to, other promoter sequences provided herein. In the context of this document, the term "operably linked" refers to such a functional relationship between a promoter or other regulatory element and the associated transcriptional polynucleotide sequence or coding sequence of a gene (or transgene) such that the function of the promoter, etc., is to initiate, maintain, alteration, induction, regulation and/or stimulation of transcription and expression of the associated coding or transcribable polynucleotide sequence, at least in a particular tissue(s), at a particular developmental stage(s), and/ or under certain other conditions. The term “plant-expressible promoter” refers to a promoter that can be used to express in a plant, plant cell, and/or plant tissue an associated coding sequence, a transgene, or a transcribable polynucleotide sequence that is operably linked to the promoter.

[69] A promoter can be classified according to various criteria related to the expression profile of a coding sequence or gene (including a transgene) operably linked to the promoter, such as constitutive, developmental, tissue-specific, inducible, etc. Promoters that initiate transcription in all or most plant tissues are called "constitutive" promoters. Promoters that initiate transcription during certain developmental periods or stages are referred to as "developmental" promoters. Promoters whose expression is upregulated in certain plant tissues relative to other plant tissues are referred to as "tissue-enhanced" or "tissue-preferred" promoters. Thus, a "tissue-preferred" promoter causes relatively greater or preferential expression in a particular plant tissue(s) but has lower levels of expression in the other plant tissue(s). Promoters that provide expression in a particular plant tissue(s) but little or no expression in other plant tissues are referred to as "tissue-specific" promoters. A promoter that allows expression in a particular cell type of a plant is referred to as a "cell type-specific" promoter. An "inducible" promoter is a promoter that initiates transcription in response to an external stimulus such as cold, drought, heat or light, or other stimuli such as injury or the application of chemicals. A promoter can also be classified in terms of its origin as heterologous, homologous, chimeric, synthetic, etc. The term "heterologous" in relation to a promoter or other regulatory sequence associated with a polynucleotide sequence (e.g., a transcriptionable DNA sequence, a coding sequence, a genome or transgene) refers to a promoter or regulatory sequence that is not naturally associated with such a polynucleotide sequence, e.g., the promoter or regulatory sequence is of a different origin than its associated polynucleotide sequence and/or does not naturally occur in the plant species subject to transformation with a promoter or regulatory sequence. In a broader sense, the term "heterologous" includes a combination of two or more DNA molecules or sequences, if such a combination does not normally occur in nature. In the context of this document, “not normally found in nature” means not found in nature without human interference. For example, two or more DNA molecules or sequences will be heterologous with respect to each other if they are normally found in different genomes or at different loci in the same genome, or if they are not combined in an identical manner in nature. In accordance with many embodiments of the invention, a plant expression promoter operably linked to a polynucleotide sequence encoding an FT protein is heterologous to a polynucleotide sequence encoding an FT protein.

[70] Embodiments of the present invention provide a recombinant DNA molecule, vector, or construct comprising a florigenic FT transgene or a coding sequence operably linked to a promoter that is functional in a plant, which can be introduced or transformed into a plant to alter the associated flowering, generative, or yield trait or phenotype. Embodiments of the present invention provide a recombinant DNA molecule comprising an FT transgene or coding sequence operably linked to a "vegetative stage" promoter such that, when introduced or transformed into a plant, the expression of the FT transgene is expressed at earlier stages of plant development (i.e., during phase of vegetative growth of the plant) to obtain an increased level of FT in target tissues compared to what would otherwise be in a wild-type plant at the same stage of development. The timing of FT transgene expression during the vegetative stage(s) of development may be important to influence one or more generative, flowering and/or yield related traits or phenotypes by providing a temporally inductive signal to generate increased numbers of floral meristems and productive flowers in one or more nodes of the plant. Expression at the vegetative stage may be needed to initiate early flowering and possibly improve generative, flowering and/or yield related traits or phenotypes such as more flowers, pods, etc. per plant node. Without wishing to be bound by theory, it has been suggested that expression of the FT transgene in a vegetative stage plant may result in synchronization and/or increased early flowering at one or more nodes to produce more flowers per plant node. The promoters described below as part of this invention provide options for regulating the timing of FT expression.

[71] As used herein, a "vegetative stage" promoter includes any promoter that initiates, causes, regulates, etc., the transcription or expression of an associated gene, transgene, or transcriptionable DNA sequence during one or more of the vegetative stages of development. plants, for example, during one or more of Ve, Vc, V1, V2, V3, V4, etc., and/or any or all of the later vegetative stages of development (eg, up to the V _n stage). In other words, the term "vegetative stage" refers to the vegetative stage(s) of development of the plant as a whole. Such a "vegetative stage" promoter can be further defined as initiating, causing, regulating, etc., the transcription or expression of an associated gene/transgene or DNA sequence suitable for transcription in one or more vegetative plant tissues, such as one or more vegetative tissues. meristematic tissues. Such a "vegetative stage" promoter can be further defined as a "vegetative stage preferred" promoter that initiates, causes, regulates, etc., the transcription or expression of an associated gene, transgene or DNA sequence suitable for transcription at least preferably or mainly, if not exclusively, during one or more of the vegetative stages of the plant (as opposed to the generative stages). Moreover, each of the "vegetative stage" promoter and the "preferred vegetative stage" promoter can also allow, allow, cause, regulate, etc., the transcription or expression of its associated gene, transgene or DNA sequence suitable for transcription during generative (s) phase (s) or stage (s) of development in one or more cells or tissues of a plant, for example, in one or more floral or generative tissues. In fact, a "vegetative stage" promoter may even initiate, cause, regulate, etc., the transcription or expression of its associated gene, transgene, or transcriptional DNA sequence in one or more generative or floral tissues at a higher level or to a greater extent. than in the vegetative tissue(s), provided that the "vegetative stage" promoter also initiates, causes, regulates, etc., the transcription or expression of its associated gene, transgene or DNA sequence suitable for transcription during the time of one or more vegetative stages of plant development.

[72] The traits and characteristics associated with vegetative stages of development for a given plant species are known in the art. In the case of dicotyledonous plants, vegetative morphological characters and characteristics of the plant during vegetative stages of development may include specific cotyledon shape, presence of vegetative meristems (apical, lateral/axillary and root), leaf arrangement, leaf shape, leaf edge shape, leaf venation, petioles, stipules , bells, hypocotyl genu and roots. In accordance with embodiments of the present invention, a "vegetative stage" promoter can also be further defined by the specific vegetative stage during which it first causes, initiates, etc. observed or expressed transcription or expression of its associated gene (or transgene). For example, a vegetative stage promoter can be a Vc stage promoter, a V1 stage promoter, a V2 stage promoter, a V3 stage promoter, etc. Therefore, a “Vc stage” promoter is defined as a vegetative stage promoter that first initiates or causes transcription of its associated gene, transgene or DNA sequence available for transcription during the Vc stage of plant development, a "stage V1" promoter is defined as a vegetative stage promoter that first initiates or causes the transcription of its associated gene, transgene or DNA sequence available for transcription during the V1 stage of development plant, a "stage V2" promoter is defined as a vegetative stage promoter that first initiates or causes transcription of its associated gene, transgene, or DNA sequence suitable for transcription during the V2 stage of plant development, etc., although the expression of the associated gene, transgene, or DNA sequence suitable for trans Violations may occur continuously or intermittently in one or more tissues during the later vegetative (and/or generative) stages of development. One skilled in the art will be able to determine the timing of expression of a given gene, transgene, or DNA sequence suitable for transcription during plant development using various molecular analysis methods and techniques known in the art.

[73] According to embodiments of the present invention, the "vegetative stage" promoter may include a constitutive, tissue-preferred, or tissue-specific promoter. For example, a vegetative stage promoter can regulate the expression of an associated FT gene/transgene or DNA sequence suitable for transcription in one or more plant tissues, e.g., one or more of roots, stems, leaves, meristems, etc., during vegetative growth. (s) stage (s) of plant development. However, such a vegetative stage promoter may preferably regulate the expression of an associated FT transgene or a coding sequence or a DNA sequence suitable for transcription in one or more plant meristems. According to many embodiments of the invention, the "vegetative stage" promoter can be a "meristem-specific" or "meristem-preferred" promoter that causes the expression of an FT transgene or a coding sequence or DNA sequence suitable for transcription in a meristematic tissue. FT proteins are known to function in the plant meristem to help initiate the transition from vegetative to generative growth following translocation of the FT protein from leaves. In contrast, embodiments of the present invention provide expression of an FT transgene directly in the plant meristem to induce flowering and impart an altered generative and/or yield related trait or phenotype to the plant. Thus, according to embodiments of the present invention, there is provided a recombinant DNA molecule, construct, or vector comprising an FT transgene or a coding sequence operably linked to a "meristem-specific" or "meristem-preferred" promoter that regulates the expression of the FT transgene at least preferably in one or more meristematic tissues of the plant, upon transformation into a plant. As used herein, "meristem-preferred promoter" refers to promoters that preferentially cause the expression of a linked gene, transgene, or DNA sequence suitable for transcription in at least one meristematic plant tissue over other plant tissues, e.g., one or more apical and/or axillary meristems, while "meristem-specific promoter" refers to promoters that cause the expression of a linked gene, transgene or transcriptionable DNA sequence exclusively (or almost exclusively) in at least one meristem tissue of a plant.

[74] According to embodiments of the present invention, a recombinant DNA molecule is provided comprising an FT coding sequence operably linked to a vegetative stage promoter, which may also be a meristem-preferred and/or meristem-specific promoter. For example, the promoter may include the pAt. Erecta promoter from Arabidopsis (SEQ ID NO: 31) or a functional fragment or portion thereof. Two examples of a truncated portion of the pAt. Erecta promoter according to embodiments of this are given as SEQ ID NO: 32 and SEQ ID NO: 48. See, for example, Yokoyama, R. et al., ʺThe Arabidopsis ERECTA gene is expressed in the shoot apical meristem and organ primordia, The Plant Journal 15 (3): 301-310 (1998). pAt.Erecta is an example of a vegetative stage promoter that is also meristem-preferred. Other meristem-preferred or meristem-specific vegetative stage promoters have been identified based on their characteristic expression profile (see, for example, Examples 4 and 7 below) and can also be used to drive FT expression in accordance with embodiments of the present invention. For example, promoters from the following receptor-like kinase (RLK) genes have been identified that can be used as meristem-preferred vegetative stage promoters: Glyma10g38730 (SEQ ID NO: 33), Glyma09g27950 (SEQ ID NO: 34), Glyma06g05900 (SEQ ID NO: : 35) and Glyma17g34380 (SEQ ID NO: 36) and any functional parts thereof. Meristem-preferred vegetative stage promoters according to embodiments of the present invention may also include promoters of potato receptor-like kinase (RLK) genes: PGSC0003DMP400032802 (SEQ ID NO: 37) and PGSC0003DMP400054040 (SEQ ID NO: 38) and any functional portions thereof . Based on the characterization of the pAt.Erecta promoter driving FT expression herein and similar expression profiles defined for other RLK, Erecta or Erecta -like ( Erl ) genes, meristem-preferred or meristem-specific vegetative stage promoters of the invention may additionally include any known or later identified promoter sequences for the RLK, Erecta and Erecta-like genes from other dicotyledonous species having a vegetative stage expression profile in the plant meristem.

[75] Additional examples of meristem-preferred or meristem-specific promoters may include promoters from the following Arabidopsis genes: Pinhead ( At.PNH ) (SEQ ID NO: 39), Angustifolia 3 or At.AN3 (SEQ ID NO: 40), At .MYB17 ( At.LMI2 or Late Meristem Identity 2 ; At3g61250) (SEQ ID NO: 41), Kinesin-like gene (At5g55520) (SEQ ID NO: 42), AP2/B3-like genes including At.REM17 (SEQ ID NO: 43) or At.REM19 , and Erecta-like genes 1 and 2, At.Erl1 (SEQ ID NO: 44) and At.Erl2 (SEQ ID NO: 45) and any functional portions thereof. Another example is the At.AP1 promoter (pAt.AP1 or pAP1) from Arabidopsis (SEQ ID NO: 49) or a functional part thereof. However, the pAt.AP1 promoter can be considered as a later vegetative and generative stage promoter. Given the late expression profile in the vegetative and generative stages, the pAt.AP1 promoter and related promoters can be used to direct the expression of the FT transgene and/or the suppressor element of the FT transgene. Additional examples identified from similar genes and/or genes having a similar expression profile to the pAt.AP1 promoter in native plant species may include a promoter from one of the following genes: AT1G26310.1 (SEQ ID NO: 50), AT3G30260.1 ( SEQ ID NO: 51) or AT5G60910.1 (SEQ ID NO: 52) from Arabidopsis ; Glyma01g08150 (SEQ ID NO: 53), Glyma02g13420 (SEQ ID NO: 54), Glyma08g36380 (SEQ ID NO: 55) or Glyma16g13070 (SEQ ID NO: 56) from soy; Solyc02g065730 (SEQ ID NO: 57), Solyc02g089210 (SEQ ID NO: 58), Solyc03g114830 (SEQ ID NO: 59) or Solyc06g069430 (SEQ ID NO: 60) from tomato; or GRMZM2G148693 (SEQ ID NO: 61), GRMZM2G553379 (SEQ ID NO: 62), GRMZM2G072582 (SEQ ID NO: 63), or GRMZM2G147716 (SEQ ID NO: 64) from corn, or any functional part thereof.

[76] The vegetative stage promoter, which can also be a meristem-preferred and/or meristem-specific promoter, can include both early and late vegetative stage promoters depending on their expression profile during the vegetative stages of development. The "early vegetative stage" promoter first initiates or causes observable or pronounced transcription or expression of its associated gene/transgene or transcriptionable DNA sequence during one or more of the early vegetative stages (i.e. Ve-V5 stages), while the promoter "late vegetative stage" first initiates or causes observable or pronounced transcription or expression of its associated gene/transgene or transcriptionable DNA sequence during one or more of the later vegetative stages (i.e., stages V6 and later). An early or late vegetative stage promoter may also be a preferred early or late vegetative stage promoter. An "early vegetative stage preferred" promoter initiates, causes, regulates, etc., the transcription or expression of its associated gene/transgene or transcriptionable DNA sequence predominantly or to a greater extent during one or more of the early vegetative stages (i.e., e. stages Ve-V5) compared to later vegetative stages. Similarly, a "late vegetative stage preferred" promoter initiates, causes, regulates, etc., the transcription or expression of its associated gene/transgene or transcriptionable DNA sequence predominantly or to a greater extent during one or more late vegetative stages ( i.e. stages V6 and later) compared to earlier vegetative stages. Accordingly, an early vegetative stage promoter may also be a preferred late vegetative stage promoter if the promoter first initiates or causes observable or pronounced transcription or expression of an associated gene/transgene or DNA sequence suitable for transcription during the earlier vegetative stages, but also initiates, causes, regulates, etc., the transcription or expression of an associated gene/transgene or DNA sequence suitable for transcription predominantly or to a greater extent during the later vegetative stages. The examples of vegetative stage promoters listed above may include early and late vegetative stage promoters, which may also be early vegetative stage preferred or late vegetative stage preferred.

[77] The polynucleotide sequence of a vegetative stage promoter (or a functional fragment or portion thereof) may also have loose sequence identity to any of the above vegetative stage promoters and still retain a similar or identical expression profile of the associated transcriptional DNA sequence, gene or transgene, functional associated with the promoter. For example, a vegetative stage promoter may comprise a polynucleotide sequence that is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identical to a polynucleotide sequence selected from the above SEQ ID NOs: 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63 or 64, or her functional part. A "functional portion" of a known or proposed promoter sequence is defined as one or more continuous or discontinuous portions of a known or proposed promoter sequence that can functionally regulate, cause, stimulate, etc., the expression of its associated gene, transgene or DNA sequence suitable for transcription. in an identical or similar manner to a known or proposed promoter sequence. In view of the present invention, one skilled in the art will be able to determine whether a promoter containing one or more parts of a known or proposed promoter sequence and/or having a shorter sequence or a sequence with less strict sequence identity relative to a known or proposed promoter sequence results in a similar expression profile and/or similar phenotypes or effects when its associated reporter gene or FT transgene is expressed in a plant, compared to a known or proposed promoter sequence.

[78] In accordance with some embodiments of the invention, a "generative stage" promoter (defined below) can be operably linked and used to express an FT transgene or coding sequence, provided that the generative stage promoter provides (i.e., initiates, causes, regulates, etc.) at least some level of expression of the FT transgene during the vegetative stage(s) of plant development to provide an early flowering induction signal. Examples of generative stage promoters are shown below. Whether a given promoter is categorized as an early or late vegetative stage promoter and/or a generative stage promoter depends on the particular plant species in which the promoter is used. A promoter that has a particular expression profile in one plant species, e.g., in its native plant species, may have a different, altered, or shifted expression profile when expressed in another plant species (e.g., heterologously in another plant species), although it is contemplated that the expression profile with a given promoter will often be similar (if not identical or nearly identical) across plant species. For example, a generative stage promoter in one plant species can function as an early vegetative stage promoter when used to express a transgene or transcriptionable DNA sequence in another plant species. Thus, the generative stage promoter can be used in some cases heterologously to express the FT transgene and induce early flowering. For example, the pAt.AP1 promoter (SEQ ID NO: 49) has a more generative stage preferred expression profile in its native Arabidopsis plant species, but can regulate an early vegetative stage expression profile in the meristem when applied heterologously to soybean plants in addition to regulating expression at generative stage.

[79] As indicated above, a recombinant DNA molecule, construct, or vector of the invention may comprise an expression cassette containing a polynucleotide sequence encoding an FT protein (i.e., an FT transgene) that is operably linked to a vegetative stage promoter, which may also be meristem-preferred and/or meristem-specific promoter. The coding polynucleotide sequence of the FT transgene or expression cassette can also be operably linked to one or more additional regulatory elements such as enhancers, leader sequences, transcription initiation sites (TSITs), linkers, 5' and 3' untranslated regions, introns, polyadenylation signals, termination regions or sequences, etc., that are appropriate or necessary to regulate or allow expression of the FT transgene or cassette for efficient production of the FT protein in the plant cell. Such additional regulatory elements may be optional and used to increase or optimize the expression of the transgene. For the purposes of this invention, an "enhancer" can be distinguished from a "promoter" in that an enhancer typically lacks a transcription initiation site, TATA box, or equivalent sequence, and is therefore insufficient alone to direct transcription. In the context of this document, a "leader sequence" can be generally defined as the DNA sequence of the untranslated 5' region (5' UTR) of a gene (or transgene) between the transcription initiation site (TSI) and the start site of the protein coding sequence.

[80] As used herein in relation to a polynucleotide, a "construct" is a segment or sequence of a polynucleotide containing one or more sequence elements, such as a coding sequence or a DNA sequence suitable for transcription, and one or more expression or regulatory elements, such as a promoter. , enhancer, etc. An "expression cassette" is a type of construct containing a transcriptional coding or DNA sequence that can express a transcriptional coding or DNA sequence in a suitable host, such as a plant or bacterial cell, and one or more promoters and/or regulatory elements operably linked to a coding sequence or DNA sequence suitable for transcription. A "vector" is a polynucleotide or DNA molecule which may contain one or more constructs and/or expression cassettes and which is suitable for stability, storage or other uses or purposes such as delivery, transformation and/or maintenance in a plant or host cell. . A "vector" may include a plasmid or circular DNA molecule, a linear DNA molecule, a transformation vector suitable for plant transformation, etc. A DNA molecule or vector may contain one or more constructs, expression cassettes, selectable markers, replication elements, and/or support, etc.

[81] In accordance with embodiments of the present invention, the term "recombinant" in relation to a polynucleotide molecule (DNA or RNA), protein, construct, vector, etc. refers to a polynucleotide or protein molecule or sequence that is not normally found in nature and / or is under conditions in which it is not normally found in nature, including a polynucleotide molecule (DNA or RNA), protein, construct, etc., containing a combination of two or more polynucleotide or protein sequences that would not be contiguous in nature or would not be close to each other without human intervention, and/or a DNA molecule, construct, etc., containing at least two DNA sequences that are heterologous with respect to each other. A recombinant DNA molecule, construct, etc. may contain DNA sequences that are separate from other polynucleotide sequences that are found in the vicinity of such DNA sequences in nature, and/or a DNA sequence that is contiguous (or contiguous) with other polynucleotide sequences that are under natural conditions are not close to each other. Recombinant DNA molecule, construct, etc. can also refer to a DNA molecule or sequence that has been genetically designed and constructed outside of a cell. For example, a recombinant DNA molecule may include a suitable plasmid, vector, etc., and may include a linear or circular DNA molecule. Such plasmids, vectors, etc. may contain various accessory elements, including a prokaryotic origin and a selection marker, as well as an FT-expressing transgene or expression cassette, possibly in addition to a plant selection marker gene, etc.

[82] According to embodiments of the present invention, a second expression cassette is provided comprising a transcriptionable polynucleotide or DNA sequence operably linked to a promoter suitable for expression in plants, wherein the transcriptionable DNA sequence contains a sequence that corresponds to at least part of the FT transgene and/or its complementary sequence and targets the FT transgene for suppression. The transcribed DNA sequence may encode an RNA molecule containing a targeting sequence that is complementary to at least a portion of the pre-mRNA or mature mRNA encoded by a polynucleotide sequence encoding an FT protein (i.e., an FT transgene), such that the RNA molecule represses the FT transgene. Accordingly, a recombinant DNA molecule, construct or vector for transformation into a plant is provided, comprising a second expression cassette. Such recombinant DNA molecule, construct, or vector may further comprise a first expression cassette containing a coding polynucleotide sequence encoding an FT protein (i.e., an FT transgene) operably linked to a first promoter suitable for expression in plants, and wherein the second expression cassette contains a DNA sequence suitable for transcription, operably linked to a second promoter suitable for expression in plants. Alternatively, two recombinant DNA molecules, constructs, or two vectors for plant transformation may be provided, comprising a first recombinant DNA molecule, construct, or vector and a second recombinant DNA molecule, construct, or vector, wherein the first recombinant DNA molecule, construct, or vector comprises the first expression cassette containing the FT transgene, and the second recombinant DNA molecule, construct or vector contains a second expression cassette containing a DNA sequence suitable for transcription, which contains a sequence that corresponds to at least part of the FT transgene and/or a complementary sequence with it. In some embodiments, a plant-expressible promoter is operably linked to a transcriptionable DNA sequence that encodes an RNA molecule to suppress the FT gene, or the transgene is heterologous to a transcriptionable DNA sequence.

[83] Any method known in the art can be used to suppress the FT transgene according to embodiments of the present invention, including expression of antisense RNAs, double-stranded RNAs (dsRNAs), or inverted repeat RNA sequences, or by co-suppression or RNA interference ( RNAi) through the expression of small interfering RNAs (siRNAs), short hairpin RNAs (shRNAs), trans-acting siRNAs (td-miRNAs), or micro-RNAs (miRNAs). See, for example, US Patent Application Publication Nos. 2009/0070898, 2011/0296555, and 2011/0035839, the contents of which are incorporated herein by reference. Accordingly, an RNA molecule encoded by a DNA sequence suitable for transcription can be antisense RNA, double-stranded RNA (dsRNA) or inverted repeat RNA, small interfering RNA (siRNA), short hairpin RNA (shRNA), trans-acting siRNA (td- siRNA) or microRNA (miRNA), including precursor RNAs such as siRNA or microRNA precursors that can be processed or cleaved into a mature RNA molecule, such as a mature siRNA or microRNA. In the context of this document, the term "suppression" refers to the reduction, reduction or elimination of the level of expression of an mRNA and/or protein encoded by a target gene and/or transgene in a plant, plant cell or plant tissue, which may be limited to a particular tissue and/or developmental stage. plants depending on the promoter used to express the RNA molecule.

[84] In accordance with embodiments of the present invention, a recombinant DNA molecule, construct, or vector is provided, containing a DNA sequence suitable for transcription and/or suppression elements encoding an RNA molecule or sequence targeting an FT transgene for suppression, wherein the DNA sequence is suitable for transcription is operably linked to a promoter suitable for expression in plants. Since the RNA molecule is intended for suppression, the RNA molecule encoded by a DNA sequence suitable for transcription may be a non-coding RNA molecule. For the purposes of this invention, a "non-coding RNA molecule" is an RNA molecule that does not code for a protein. In accordance with some embodiments of the invention, the recombinant DNA molecule, construct, or vector may comprise a first expression cassette containing an FT transgene and a second expression cassette containing a DNA sequence suitable for transcription encoding an RNA molecule that targets the FT transgene for suppression. Alternatively, the first expression cassette containing the FT transgene and the second expression cassette containing a DNA sequence suitable for transcription to suppress the FT transgene may be in two different recombinant DNA molecules, constructs or vectors.

[85] The DNA sequence to be transcribed may comprise a suppression element that is at least 15 nucleotides in length, e.g., about 15 nucleotides to about 27 nucleotides in length, or 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 or 27 nucleotides, wherein the suppression element corresponds to at least a portion of the target FT transgene to be suppressed and/or its complementary DNA sequence. In many embodiments, a transcriptional DNA sequence or suppression element may be at least 17, at least 19, at least 20, at least 21, at least 22, or at least 23 nucleotides in length (or more) (for example, a length of at least 25, at least 30, at least 50, at least 100, at least 200, at least 300, at least 500, at least 1000, at least 1500, at least 2000, at least 3000, at least 4000 or at least 5000 nucleotides). Depending on the length and sequence of the DNA sequence available for transcription or the suppression element, one or more sequence mismatches or base mismatches can be tolerated without loss of suppression function. In fact, even short RNAi suppressor elements ranging in length from about 15 nucleotides to about 27 nucleotides can have one or more mismatches or non-complementary bases yet effectively suppress the target FT transgene. Accordingly, the sense or antisense suppression element may contain a sequence that is at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% identical or complementary to the corresponding sequence of at least a segment or portion of the target FT transgene or its complementary sequence, respectively.

[86] The transcribed DNA sequence of the present invention for targeted suppression of an FT transgene may comprise one or more of the following suppression elements and/or targeting sequences: (a) a DNA sequence that contains at least one antisense DNA sequence that is antisense or complementary at least one segment or one portion of the target FT transgene; (b) a DNA sequence that contains multiple copies of at least one antisense DNA sequence that is antisense or complementary to at least one segment or one portion of the target FT transgene; (c) a DNA sequence that contains at least one sense DNA sequence that contains at least one segment or one portion of the target FT transgene; (d) a DNA sequence that contains multiple copies of at least one sense DNA sequence, each of which contains at least one segment or one part of the target FT transgene; (e) a DNA sequence that contains a segment or portion of the target FT transgene with inverted repeats and/or is transcribed into RNA to suppress the target FT transgene by forming a double-stranded RNA, wherein the transcribed RNA contains at least one antisense DNA sequence that is antisense or complementary to at least one segment or one portion of the target FT transgene, and at least one sense DNA sequence that contains at least one segment or one portion of the target FT transgene; (f) a DNA sequence that is transcribed into RNA to suppress the target FT transgene by producing a single double-stranded RNA and contains a plurality of antisense DNA sequences, each of which is antisense or complementary to at least one segment or one portion of the target FT transgene, and a plurality of sense sequences DNA, each containing at least one segment or one portion of the target FT transgene; (g) a DNA sequence that is transcribed into RNA to suppress the target FT transgene by generating a plurality of double-stranded RNAs and contains a plurality of antisense DNA sequences, each of which is antisense or complementary to at least one segment or one portion of the target FT transgene, and a plurality of sense sequences DNA, each containing at least one segment or one portion of the target FT transgene, wherein a plurality of antisense DNA segments and a plurality of sense DNA segments are ordered in a series of inverted repeats; (h) a DNA sequence that contains nucleotides derived from microRNA, preferably plant microRNA; (i) a DNA sequence that contains nucleotides from the siRNA; (j) a DNA sequence that is transcribed into an RNA aptamer capable of binding to a ligand; and (k) a DNA sequence that is transcribed into an RNA aptamer capable of binding to the ligand and a DNA that is transcribed into a regulatory RNA capable of regulating the expression of the target FT transgene, where regulation of the target FT transgene is dependent on the conformation of the regulatory RNA, and conformation regulatory RNA has an allosteric effect, the state of binding of the RNA aptamer by the ligand. The transcribed DNA sequence may contain one or more of the above suppression elements and/or one or more targeting sequences, which may correspond to one or more sequences of the FT transgene and/or its complementary sequence.

[87] Multiple sense and/or antisense sequences of suppression of a DNA sequence suitable for transcription for one or more target sequences of the FT transgene can be ordered periodically in tandem or ordered into tandem segments or repeats, such as tandem inverted repeats, which can also be interrupted by one or more more spacer sequences. In addition, the sense or antisense sequence of a transcriptionable DNA sequence or suppression element may ideally not match or be complementary to the target sequence of the FT transgene, depending on the sequence and length of the transcriptionable DNA sequence or suppression element. Indeed, even short RNAi suppressor elements with a length ranging from about 15 nucleotides to about 27 nucleotides may have one or more mismatches or non-complementary bases depending on the length of the suppression element or targeting sequence, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 (or more) mismatches, yet effectively suppress the target FT transgene. Accordingly, the sense or antisense suppression element may contain a sequence that is at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% identical or complementary to the corresponding sequence of at least a segment or portion of the target FT transgene or its complementary sequence, respectively.

[88] In the case of antisense suppression, a DNA sequence suitable for transcription may contain a sequence that is antisense or complementary to at least a portion or segment of the target FT transgene. The transcribed DNA sequence and/or suppression element may comprise a plurality of antisense sequences that are complementary to one or more portions or one or more segments of the target FT transgene, or multiple copies of an antisense sequence that is complementary to the target FT transgene. The antisense sequence can be at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% identical DNA sequence that is complementary to at least a segment or portion of the target FT transgene mRNA. In other words, the antisense sequence can be at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% complementary to the target FT transgene.

[89] As used herein, the terms "percent complementarity" or "proportion of complementarity" in relation to two nucleotide sequences are similar in concept to percentage identity, but refer to the percentage of nucleotides in a sequence that exhibit optimal base pairing or hybridize with nucleotides of a reference sequence when the two sequences are ordered linearly and exhibit optimal base pairing without secondary folds such as loops, stems, or hairpins, but allowing for mismatches and gaps in base pairing between the two sequences. This percentage of complementarity can be observed between two DNA strands, two RNA strands, or a DNA strand and an RNA strand. "Percent complementarity" can be calculated by (i) optimally base-pairing or hybridizing two nucleotide sequences in a linear and fully unfolded ordering (i.e., no folding or secondary structures) in a comparison window (e.g., an alignment window), (ii) determining the number positions at which there is a base pairing between two sequences in the comparison window to obtain the number of complementary positions, (iii) dividing the number of complementary positions by the total number of positions in the comparison window, and (iv) multiplying this result by 100% to obtain the percent complementarity of the two sequences. For this purpose, the alignment window is defined as the region of complementarity between two sequences. Optimal base pairing between two sequences can be determined based on known nucleotide base pairings such as G-C, A-T and A-U via hydrogen bonding. If "percent complementarity" is calculated relative to a reference or query sequence without specifying a specific comparison window, then percent identity is determined by dividing the number of complementary positions between two linear sequences by the total length of the reference sequence. For the purposes of this invention, when two sequences (query and subject) have optimal base pairing (allowing for gaps and mismatches or unpaired nucleotides), the "percent complementarity" for the query sequence (when the comparison window is not defined) is equal to the number of paired positions between the two sequences, divided by the total number of positions in the requested sequence along its entire length, which is then multiplied by 100%.

[90] For suppression of an FT transgene by an inverted repeat or transcribed dsRNA, a DNA sequence suitable for transcription may contain a sense sequence that contains a segment or portion of the target FT transgene and an antisense sequence that is complementary to the segment or portion of the target FT transgene, wherein the sense and antisense DNA sequences are arranged in tandem. Each of the sense and antisense sequences, respectively, may be less than 100% identical or complementary to a segment or portion of the target FT transgene, as described above. The sense and antisense sequences can be separated by a spacer sequence such that an RNA molecule transcribed from a transcriptionable DNA sequence forms a stem, loop, or stem-loop structure between the sense and antisense sequences. Otherwise, a DNA sequence suitable for transcription may contain a plurality of sense and antisense sequences that are arranged in tandem, which may also be separated by one or more spacer sequences. A transcribed DNA sequence containing a plurality of sense and antisense sequences can be ordered as groups of sense sequences followed by groups of antisense sequences, or as groups of sense and antisense sequences arranged in tandem.

[91] For suppression of the FT transgene by micro-RNA (miRNA), a DNA sequence suitable for transcription may comprise a DNA sequence derived from a microRNA sequence native to a virus or eukaryote, such as an animal or plant, or modified or derived from such a native sequence. miRNA. Such native or native-derived miRNA sequences may form an in-itself hybridization structure and serve as a scaffold for a precursor microRNA, and may also correspond to the stem region of a native miRNA precursor sequence, such as in the native (or native-derived) sequence miRNA or pre-miRNA. Moreover, in addition to these native or derived from native backbone or preprocessed miRNA sequences, the engineered miRNAs according to the invention additionally contain a sequence corresponding to a segment or part of the target FT transgene. Thus, in addition to the preprocessed or framework sequences, the suppression element can be designed to further contain a sense and/or antisense sequence that corresponds to a segment or portion of the target FT transgene and/or a sequence that is complementary to it, although one or more mismatches in the sequence.

[92] Constructed miRNAs are applicable for targeted gene suppression with increased specificity. See, for example, Parizotto et al., Genes Dev . 18:2237-2242 (2004), and US Patent Application Publication Nos. 2004/0053411, 2004/0268441, 2005/0144669, and 2005/0037988, the contents of which are incorporated herein by reference. microRNAs are non-coding RNAs. Cleavage of the precursor miRNA molecule produces a mature miRNA that is typically about 19 to about 25 nucleotides in length (typically about 20 to about 24 nucleotides in plants), e.g., 19, 20, 21, 22, 23 , 24 or 25 nucleotides, and has a sequence corresponding to the target suppression gene and/or its complementary sequence. Mature miRNA hybridizes to target mRNA transcripts and directs the binding of a protein complex to target transcripts, which can inhibit translation and/or result in transcript degradation, thus downregulating or suppressing target gene expression. microRNA precursors are also useful in plants to direct the in-phase production of miRNAs, trans-acting siRNAs (tg-miRNAs) in a process that requires an RNA-dependent RNA polymerase to suppress the target gene. See, for example, Allen et al., Cell 121:207-221 (2005), Vaucheret Science STKE , 2005:pe43 (2005), and Yoshikawa et al. Genes Dev ., 19:2164-2175 (2005), the contents of which are incorporated herein by reference.

[93] In accordance with embodiments of the present invention, a recombinant DNA molecule, construct, or vector containing a DNA sequence suitable for transcription encoding a microRNA or microRNA precursor molecule for targeted suppression of an FT transgene is provided. Such a DNA sequence suitable for transcription may contain a sequence of at least 19 nucleotides in length that corresponds to the FT transgene and/or a sequence complementary to the FT transgene, although one or more sequence mismatches and/or mismatches are allowed.

[94] The FT transgene can also be suppressed by one or more small interfering RNAs (siRNAs). The siRNA pathway involves the out-of-phase cleavage of a longer intermediate double-stranded RNA ("RNA double helix") into small interfering RNAs (siRNAs). The size or length of siRNAs typically range from about 19 to about 25 nucleotides or base pairs, but common classes of siRNAs include those of 21 base pairs or 24 base pairs. Thus, a DNA sequence suitable for transcription according to the present invention may encode an RNA molecule of at least about 19 to about 25 nucleotides in length, such as 19, 20, 21, 22, 23, 24, or 25 nucleotides in length. For siRNA suppression, a recombinant DNA molecule, construct or vector containing a DNA sequence suitable for transcription and/or a suppression element encoding an siRNA molecule for targeted suppression of the FT transgene is proposed.

[95] In accordance with embodiments of the present invention, a recombinant DNA molecule, vector, or construct is provided that contains a DNA sequence suitable for transcription encoding a non-coding RNA molecule that binds or hybridizes to a target mRNA sequence in a plant cell, such as encoding (exon) and /or untranslated (NTR) sequence of the target mRNA, wherein the target mRNA molecule encodes the FT protein, and the DNA sequence suitable for transcription is operably linked to a promoter suitable for expression in plants. In addition to targeting a mature mRNA sequence, a non-coding RNA molecule encoded by a DNA sequence suitable for transcription can be targeted to the intron sequence of a transgene or FT transcript. In accordance with other embodiments of the invention, a recombinant DNA molecule, vector, or construct is provided that contains a DNA sequence suitable for transcription encoding a non-coding RNA (precursor) molecule that is cleaved or processed into a mature non-coding RNA molecule that binds or hybridizes to a target mRNA in a cell. plants, wherein the target mRNA molecule encodes the FT protein, and the DNA sequence suitable for transcription is operably linked to a promoter suitable for expression in plants, which may be tissue-specific, tissue-preferential, developmental, and/or another type of promoter.

[96] According to embodiments of the present invention, a recombinant DNA molecule, vector, or construct is provided, comprising a DNA sequence suitable for transcription encoding a non-coding RNA molecule, wherein the non-coding RNA molecule is at least 80%, at least 85% , at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5 % or 100% complementary to at least a segment or portion of an mRNA molecule (i) expressed from an FT transgene and/or (ii) encoding an FT protein in a plant or plant cell, wherein a DNA sequence suitable for transcription is operably linked to a promoter suitable for expression in plants. The non-coding RNA molecule can be targeted to the mature mRNA or precursor mRNA sequence, the 5' or 3' untranslated region (UTR), the coding sequence (exon) and/or the intron or intron sequence of the transgene or FT transcript. In accordance with some embodiments of the invention, the non-coding RNA molecule targets the FT transgene for suppression and is at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% complementary to at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 26, or at least 27 consecutive nucleotides of a polynucleotide (coding) sequence encoding an FT protein (e.g., SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, or 29), or any other known coding sequence for florigenic FT. In accordance with other embodiments of the invention, the non-coding RNA molecule is at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% complementary to at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 26, or at least 27 consecutive nucleotides of an mRNA molecule encoding an FT protein that is at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30, or any th other known florigenic FT protein sequence or a functional fragment thereof. In the context of this document, the term "sequential" in relation to a polynucleotide or protein sequence means the absence of deletions or gaps in the sequence. In accordance with embodiments of the present invention, the non-coding RNA molecule encoded by a DNA sequence suitable for transcription of the recombinant DNA molecule, vector or construct provided herein may be a mature miRNA or miRNA, or a precursor microRNA or miRNA that can be processed or be cleaved in the plant cell to form mature miRNA or miRNA.

[97] In some embodiments, a DNA sequence suitable for transcription may comprise a sequence encoding a targeting sequence of an RNA molecule that is complementary to and/or hybridizes with mRNA of a particular Gm.FT2a gene or transgene to target the Gm.FT2a gene or transgene. for suppression. The DNA sequence to be transcribed may comprise a sequence (eg, SEQ ID NO: 65) encoding a targeting sequence (eg, SEQ ID NO: 66) of an RNA molecule encoded by or transcribed from a transcribable DNA sequence. The targeting sequence of the RNA molecule may be any sequence of sufficient length that is complementary to the segment or portion of the mRNA encoded by the FT transgene, and the DNA sequence suitable for transcription may contain a sequence that encodes or is transcribed into the targeting sequence of the RNA molecule. For example, a transcriptionable DNA sequence encoding a precursor miRNA may contain SEQ ID NO: 67, which can be processed into a mature miRNA containing SEQ ID NO: 67 targeted at the Gm.FT2a gene or transgene for suppression. The mRNA encoded by the target FT gene or transgene may contain a target site for an RNA molecule encoded by a DNA sequence suitable for transcription. Such a target site in the mRNA of the FT transgene may comprise, for example, SEQ ID NO: 68, which may be encoded by the sequence (eg, SEQ ID NO: 69) of the FT gene or transgene. Thus, the coding polynucleotide sequence of an FT transgene may contain a sequence encoding a target site for an RNA molecule.

[98] In addition to a promoter suitable for expression in plants, a recombinant DNA molecule, construct, vector or expression cassette containing a DNA sequence suitable for transcription encoding a non-coding RNA molecule for suppression of the FT transgene can also be operably linked to one or more additional regulatory elements. such as enhancers, transcription initiation sites (TSITs), linkers, polyadenylation signals, 5' and/or 3' backbone or backbone sequences, termination sequences or regions, etc., which are appropriate, necessary or preferred to regulate or mediate expression DNA sequences suitable for transcription in a plant cell or tissue. Such additional regulatory elements may be optional and may be used to increase or optimize the expression of a DNA sequence suitable for transcription.

[99] In some embodiments, a DNA sequence suitable for transcription may comprise a sequence that corresponds to at least a portion of the non-coding sequence of the FT transgene and/or its complementary sequence, such as a 5' or 3' untranslated region (UTR) or the intron sequence of the FT transgene, which may allow selective suppression of the FT transgene relative to the endogenous FT gene. "FT transgene non-coding sequences" (not to be confused with "non-coding RNA molecule", encoded by a DNA sequence suitable for transcription, to suppress a FT gene or transgene) are FT transgene sequences that are transcribed and form part of the pre-mRNA and/or mature mRNA, but do not encode the transgenic FT protein. Accordingly, a DNA sequence suitable for transcription may encode an RNA molecule containing a targeting sequence that corresponds to at least a portion of the non-coding sequence of the FT transgene and/or a sequence complementary to it. Thus, a DNA sequence suitable for transcription may contain a sequence that corresponds to at least a portion of the pre-mRNA or mature mRNA encoded by the FT transgene. The sequence of the DNA sequence suitable for transcription and the encoded targeting sequence of the RNA molecule depend on the specific non-coding sequences of the FT transgene, which may be the same or different or unique relative to the endogenous FT gene(s). In accordance with some embodiments of the invention, a recombinant DNA molecule, vector, or construct is provided that contains an expression cassette that contains a DNA sequence suitable for transcription, having a sequence that corresponds to at least a portion of the non-coding sequence of the FT transgene and/or a sequence complementary to it. Similarly, as described above, two or more expression cassettes can be provided, including a first expression cassette and a second expression cassette, wherein the first expression cassette contains a polynucleotide sequence encoding an FT protein (i.e., an FT transgene) operably linked to a first promoter suitable for expression in plants, and the second expression cassette contains a DNA sequence suitable for transcription, functionally linked to a second promoter suitable for expression in plants, and the DNA sequence suitable for transcription contains a sequence that corresponds to at least part of the non-coding sequence of the FT transgene and/or its complementary sequence. The first and second expression cassettes may be in the same DNA molecule, one vector or one construct, or in separate DNA molecules, vectors or constructs.

[100] A transcribed DNA sequence encoding an RNA molecule that targets the FT transgene for suppression can be operably linked to a promoter suitable for expression in plants. The expression profile of an RNA molecule may depend on the particular promoter suitable for expression in plants. As described above, expression of the FT transgene under the control of the vegetative stage promoter can be used to initiate early flowering and increase the number of flowers, pods, etc. per plant node, but it can also lead to early death of the plant. It has recently been suggested that additional expression of an RNA molecule targeted to the FT transgene for suppression could be used to correct and/or attenuate the profile and expression level of the FT transgene to further mitigate early termination phenotypes. This can be done by reducing the amount of transcript and protein from the FT transgene (ie, reducing the level of expression) and/or modifying the expression profile (ie, adjusting or restricting the FT transgene expression profile). Without being limited by theory, it is believed that a decrease in FT transgene expression may be sufficient to induce early flowering with mitigation of early termination phenotypes. Similarly, a limited spatiotemporal profile of FT expression may reduce FT expression in specific tissues and/or at specific developmental stages where the transgenic FT may cause early termination. Thus, according to embodiments of the present invention, the timing and expression profile of the FT transgene and the RNA molecule (targeting the FT transgene for suppression) may be the same, overlapping, or different.

[101] According to many of the embodiments above, at least two expression cassettes can be provided, including a first expression cassette and a second expression cassette, wherein the first expression cassette contains an FT transgene operably linked to a first promoter suitable for expression. in plants, and the second expression cassette contains a transcriptionable DNA sequence operably linked to a second promoter suitable for expression in plants, wherein the transcriptional DNA sequence encodes an RNA molecule that targets the FT transgene for suppression. The two expression cassettes may be in the same recombinant DNA molecule, construct or vector, or in separate recombinant DNA molecules, constructs or vectors. As described above, the first promoter suitable for expression in plants operably linked to the FT transgene may be a vegetative stage promoter, which may also be a meristem-specific or meristem-preferred promoter. The second promoter suitable for expression in plants, operably linked to a DNA sequence suitable for transcription, may include various types of promoters, including constitutive, inducible, developmental, tissue-specific, tissue-preferential, vegetative stage, generative stage, etc., but time and the expression profile of the RNA molecule should at least partially overlap with time and the expression profile of the FT transgene. In accordance with some embodiments of the invention, the second promoter suitable for expression in plants can be a constitutive promoter or a vegetative stage promoter to reduce the level of expression of the FT transgene. Such a constitutive or vegetative stage promoter may also be a tissue-specific or tissue-preferred promoter and/or may have broad overlap over time and expression profile of the FT transgene. Indeed, a constitutive or overlapping expression profile of a transcriptionable DNA sequence (and a suppressor RNA molecule) with respect to the FT transgene may be effective in reducing the amount or dose of FT expression, especially if the suppression of the FT transgene is imperfect or incomplete. In some cases, the FT transgene and the suppressor construct may even be operably linked to the same or similar promoters. For example, the first plant-expressible promoter driving the expression of the FT transgene may be an early or late vegetative and/or generative stage promoter, and the second plant-expressible promoter driving the expression of a transcriptionable DNA sequence encoding an RNA molecule. for suppression, may be an early or late vegetative stage and/or generative stage promoter

[102] Many examples of constitutive promoters are known in the art, such as the cauliflower mosaic virus (CaMV) 35S and 19S promoter (see, for example, US Pat. (see, for example, Holtorf, S. et al., Plant Molecular Biology , 29: 637-646 (1995) or CaMV double boosted promoter (see, for example, US Pat. see e.g. US Pat. No. 6,372,211), mirabilis mosaic virus (MMV) promoter (see e.g. U.S. Pat. No. 6,420,547), peanut chlorotic streak colimovirus promoter (see e.g. US Pat. No. 5,850,019), nopaline or octopine promoter, ubiquitin a promoter such as the soybean polyubiquitin promoter (see, for example, US Pat. No. 7,393,948), the Arabidopsis S-adenosylmethionine synthetase promoter (see, for example, 8809628), etc., or any functional portions of the above promoters, when The contents of each of the foregoing references are incorporated herein by reference. Alternatively, the second promoter suitable for expression in plants may be a vegetative and/or generative stage promoter, examples of which are provided herein.

[103] In other embodiments, a second promoter suitable for expression in plants may have a more different timing and/or expression profile, e.g., in other plant tissues and/or at different developmental stages, relative to the FT transgene. Thus, the effective spatiotemporal expression profile of the FT transgene can be modified, altered, and/or corrected depending on the relative timing and expression profiles of the FT transgene and the transcriptionable DNA sequence encoding the RNA molecule for suppressing the FT transgene (as well as the particular targeting sequence of the molecule RNA). However, in many embodiments, a transcriptionable DNA sequence (and RNA molecule) may be expressed at a later developmental stage or in a later tissue relative to the start of expression of the FT transgene, so that the FT transgene may provide an early flowering induction signal before suppression. later expression of the suppressor RNA molecule, or in other words, the FT transgene can be suppressed after the early flowering induction signal to reduce or mitigate early termination. For example, the first plant-expressible promoter driving the expression of the FT transgene may be an early vegetative stage promoter, and the second plant-expressible promoter driving the expression of a transcriptionable DNA sequence encoding an RNA molecule for suppression may be the promoter late vegetative stage and/or generative stage promoter, or the first promoter suitable for expression in plants may be a late vegetative stage promoter and the second promoter suitable for expression in plants may be a generative stage promoter. More broadly, a second plant-expressible promoter can initiate, cause, and/or regulate the expression of its associated transgene or transcriptional DNA sequence at a later developmental stage than the first plant-expressible promoter, such that the suppressor construct generally expressed after the initial FT flowering induction signal during the earlier vegetative stage(s) of development(s). Thus, the second promoter suitable for expression in plants may be a promoter of a later developmental stage than the first promoter suitable for expression in plants. For example, a second promoter suitable for expression in plants may drive expression at a later developmental stage than a first promoter suitable for expression in plants, but in the same tissue types or lineages (e.g. meristem) as the first promoter suitable for expression in plants. expression in plants. Such a vegetative stage and/or generative stage promoter may also be a tissue-specific or tissue-preferred promoter, such as a meristem-specific or meristem-preferred meristem promoter. Examples of late vegetative stage promoters are given above.

[104] By expressing a DNA sequence suitable for transcription and an RNA molecule for suppressing the FT transgene during one or more later stages of plant development relative to the FT transgene, the effective expression profile of the FT transgene can be modified, altered and/or corrected to earlier stages of development and/ or tissues relative to the expression of a single FT transgene. In some cases, expression of the FT transgene during the vegetative stage may be delayed or continued during later vegetative and/or generative stages or in later tissues in the plant. Thus, expression of an RNA molecule that targets the FT transgene for suppression can reduce the level of the FT transgene at these later stages and/or in later tissues to effectively limit the level of expression of the FT transgene to earlier developmental stages and/or tissues. As a result, the early flowering induction signal can be maintained or maintained while the later FT expression can be attenuated or reduced to avoid or slow down early termination of the remaining meristematic reserves of the plant and allow continued vegetative growth and development of the plant after flowering.

[105] As used herein, a "generative step" promoter is defined as any promoter that initiates, causes, regulates, etc., the transcription or expression of an associated gene, transgene, or transcriptionable DNA sequence during one or more generative steps. plant development, for example, during one or more of the stages of development R1, R2, R3, R4, R5, R6, R7 and/or R8. Such a "generative stage" promoter can be further defined as a "generative stage preferred" promoter that initiates, causes, regulates, etc., the transcription or expression of an associated gene/transgene or DNA sequence suitable for transcription at least preferably or primarily, if not exclusively, during one or more of the generative stages of the plant (as opposed to the vegetative stages). Moreover, each of the “generative stage” promoter and the “generative stage preferred” promoter can also initiate, provide, allow, cause, regulate, etc., the transcription or expression of its associated gene, transgene or DNA sequence suitable for transcription in the time of the vegetative phase(s) or stage(s) of development in one or more plant cells or tissues. Thus, a generative stage promoter can also be a vegetative stage promoter when expressed during both phases of development (ie, during both the vegetative and generative stages of development). Such a generative stage promoter may also be a tissue-specific or tissue-preferred promoter, such as a meristem-specific or meristem-preferred promoter. A "generative stage" promoter can be further defined as initiating, causing, regulating, etc., the transcription or expression of its associated gene/transgene or DNA sequence suitable for transcription in one or more generative tissues of the plant. Such a "generative stage" promoter can also be defined as a "floral-preferred" promoter that initiates, causes, regulates, etc., the transcription or expression of an associated gene/transgene or DNA sequence suitable for transcription at least preferably or principally. manner, if not exclusively, in at least one floral or generative tissue, such as a floral meristem, or a "floral-specific" promoter that initiates, causes, regulates, etc., transcription or expression of its associated gene/transgene, or DNA sequences suitable for transcription exclusively (or almost exclusively) in at least one floral or generative tissue. The features and characteristics of these generative stages for a given plant species are known in the art.

[106] Examples of generative stage promoters, which may also be early or late vegetative stage promoters depending on their expression profile in a given plant species, may include the following promoters from tomato genes: Sl.Nod promoter (pSl.Nod, pLe.Nod or pNod) (SEQ ID NO: 70), Sl.MADS5 promoter (pSl.MADS5, pLe.MADS5 or pMADS5) (SEQ ID NO: 71) or Sl.MADS-RIN promoter (pSl.MADS-RIN, pLe.MADS- RIN or pMADS-RIN) (SEQ ID NO: 72), or a functional part thereof. Additional examples identified as having homology and/or a similar expression profile to the pSl.MADS5 and/or pSl.MADS-RIN promoter in native plant species may include a promoter from one of the following genes: AT1G24260.1 (SEQ ID NO: 73) , AT2G45650.1 (SEQ ID NO: 74), AT3G02310.1 (SEQ ID NO: 75), or AT5G15800.1 (SEQ ID NO: 76), or AT2G03710.1 (SEQ ID NO: 77) from Arabidopsis ; Glyma05g28140 (SEQ ID NO: 78), Glyma08g11120 (SEQ ID NO: 79), Glyma11g36890 (SEQ ID NO: 80), Glyma08g27670 (SEQ ID NO: 81), Glyma13g06730 (SEQ ID NO: 82), or Glyma19g04320 (SEQ ID NO: 83) from soy; Solyc02g089200 (SEQ ID NO: 84), Solyc03g114840 (SEQ ID NO: 85), Solyc12g038510 (SEQ ID NO: 86), Solyc04g005320 (SEQ ID NO: 87) or Solyc05g056620 (SEQ ID NO: 88) from tomato; or GRMZM2G159397 (SEQ ID NO: 89), GRMZM2G003514 (SEQ ID NO: 90), GRMZM2G160565 (SEQ ID NO: 91), GRMZM2G097059 (SEQ ID NO: 92), GRMZM2G099522 (SEQ ID NO: 93) or GRMZM2G071620 (SEQ ID NO: 93) NO: 94) from corn, or any functional portion of any of the above promoters.

[107] In accordance with embodiments of the present invention, a “generative stage” promoter can also be further defined by the specific generative stage during which it first causes, initiates, etc. observed or expressed transcription or expression of its associated gene, transgene or a DNA sequence suitable for transcription. For example, a generative step promoter can be an R1 step promoter, an R2 step promoter, an R3 step promoter, etc. Therefore, an “R1 step” promoter is defined as a generative step promoter that first initiates or causes transcription of its associated gene, transgene, or DNA sequence available for transcription during the R1 stage of plant development, an "R2 stage" promoter is defined as a generative stage promoter that first initiates or causes transcription of its associated gene, transgene, or DNA sequence available for transcription during the R2 stage of plant development, etc. etc., although expression of an associated gene, transgene, or transcriptional DNA sequence may occur continuously or intermittently in one or more tissues during the later generative stages of development. The transition from vegetative to generative stage (and the start of the R1 stage) is determined according to industry standard conventions for a given crop plant (i.e. usually, as in the case of soybeans, this is the visible appearance of the first open flower on the plant). A person skilled in the art will be able to determine the expression time of a given gene, transgene or DNA sequence suitable for transcription during plant development using various molecular analysis methods and techniques known in the art if the expression time is not already known.

[108] According to embodiments of the present invention, a "generative stage" promoter may include a constitutive, tissue-preferred, or tissue-specific promoter. For example, a generative stage promoter may regulate the expression of an associated gene, transgene, or DNA sequence suitable for transcription in one or more plant tissues, e.g., one or more of roots, stems, leaves, meristems, etc. during generative ( th) stage(s) of plant development. However, such a generative stage promoter may preferentially regulate the expression of an associated gene, transgene or DNA sequence suitable for transcription in one or more meristems of the plant. In accordance with many embodiments of the invention, a "generative stage" promoter can be a "meristem-specific" or "meristem-preferred" promoter that causes the expression of its associated gene, transgene, or DNA sequence suitable for transcription in a meristematic tissue that is at least partially corresponds to the expression profile of the FT transgene and attenuates and/or corrects the expression of the FT transgene.

[109] The polynucleotide sequence of these promoters (or functional fragments or parts thereof) can also have loose sequence identity and still retain a similar or identical expression profile of the associated gene, transgene, or transcriptionable DNA sequence operably linked to the promoter. For example, a late vegetative and/or generative stage promoter may comprise a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90% %, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97% , at least 98%, at least 99%, or 100% identical to a polynucleotide sequence selected from the above SEQ ID NOs: 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80 , 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93 or 94, or a functional part thereof. A "functional portion" of a known or proposed promoter sequence is defined above.

[110] In accordance with embodiments of the present invention, an RNA molecule encoded by a second expression cassette containing a DNA sequence suitable for transcription, operably linked to a second promoter suitable for expression in plants, can cause a decrease or elimination of the level of expression of the mRNA and/or protein encoded an FT transgene in one or more plant tissues by suppressing the FT transgene. When using the second expression cassette, the level of expression of the transgenic FT transcript and/or protein can be reduced by at least 1%, at least 2%, at least 3%, at least 4%, at least 5%. %, at least 10%, at least 20%, at least 25%, at least 30%, at least 40%, at least 50%, at least 60% at least 70%, at least 75%, at least 80%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% in one or more plant tissues, such as one or more meristematic tissues, compared to the levels of the FT transgene mRNA transcript and/or protein that would be observed in the same plant tissues without a second expression cassette. FT transgene mRNA transcript and/or protein levels can be reduced by 1%-100%, 1%-75%, 1%-50%, 1%-25%, 5%-100%, 5%-95%, 5 %-90%, 5%-85%, 5%-80%, 5%-75%, 5%-70%, 5%-65%, 5%-60%, 5%-55%, 5%- 50%, 5%-45%, 5%-40%, 5%-35%, 5%-30%, 5%-25%, 5%-20%, 5%-15%, 5%-10% , 10%-100%, 10%-90%, 10%-80%, 10%-70%, 10%-60%, 10%-50%, 10%-40%, 10%-30%, 10 %-20%, 25%-100%, 25%-75%, 25%-50%, 50%-100%, 50%-75%, or 75%-100% in one or more plant tissues compared to levels mRNA transcript and/or protein of the FT transgene that would be observed in the same plant tissues without the second expression cassette.

[111] In accordance with additional variants of the invention, otherwise, the second expression cassette can be designed to encode an RNA molecule that targets the endogenous FT gene for suppression. Selective suppression of an endogenous FT gene can be achieved by targeting its coding sequence if the FT transgene has a different coding sequence from the endogenous gene (i.e., the RNA molecule may contain a targeting sequence that is complementary to at least a portion of the coding sequence of the endogenous FT gene). Alternatively, even if the coding sequences of the FT transgene and the endogenous FT gene are the same or similar, the second expression cassette can be designed to encode an RNA molecule that targets the untranslated or non-coding mRNA sequence encoded by the endogenous FT gene, for example, within 5'UTR, 3'UTR, intron and/or leader sequences of the endogenous FT mRNA transcript, if these sequences are different or absent from the FT transgene (i.e., the RNA molecule may contain a targeting sequence that is complementary to at least a portion of the untranslated or non-coding sequence of the endogenous FT gene). In accordance with these embodiments of the invention, a DNA sequence suitable for transcription of the second expression cassette can be designed in accordance with the principles proposed herein to target a specific coding or non-translated (non-coding) mRNA sequence encoded by the endogenous FT gene for suppression, instead of the mRNA sequence encoded by the FT transgene.

[112] According to another broad aspect of the present invention, a recombinant DNA molecule, construct, or vector is provided, comprising a polynucleotide sequence encoding an FT protein (i.e., an FT transgene) operably linked to a promoter suitable for expression in plants, wherein the polynucleotide sequence additionally contains a sequence encoding a target site or sensor for an endogenous RNA molecule, such as endogenous microRNA or miRNA, while the target site or sensor is located in the pre-mRNA and/or mature mRNA transcript encoded by the FT transgene, for example, within the 5' UTR , 3'UTR, intron and/or leader sequences. As used herein, a "sensor" is a target site of a small non-coding RNA in an mRNA transcript of an FT transgene that is complementary to an endogenous RNA molecule, such as an endogenous miRNA or siRNA. An endogenous RNA molecule may occur naturally in a plant cell or tissue and function to repress one or more target genes having a target site for the endogenous RNA molecule. It is contemplated herein that the FT transgene can be further engineered to contain a sequence encoding a target site or mRNA sensor for an endogenous RNA molecule such that the FT transgene is suppressed by the endogenous RNA molecule. Therefore, suppression of the FT transgene can be used to mitigate the early termination phenotypes seen when the FT transgenic is expressed alone (ie, without suppression), similar to suppression by a second expression cassette encoding a suppression RNA molecule. An endogenous RNA molecule can be any known naturally occurring small RNA molecule such as miRNA, miRNA, etc., whose function is to initiate the suppression of one or more target genes in a plant cell. According to many embodiments of the invention, an endogenous RNA molecule can be naturally expressed during late vegetative and/or generative stages of development (e.g., in one or more late vegetative, generative, and/or floral tissues) such that the endogenous RNA molecule causes suppression of the transgene. FT after providing the initial flowering induction signal. In many embodiments, the promoter suitable for expression in plants, the target site of the FT transgene, or both are heterologous with respect to the coding polynucleotide sequence of the FT transgene.

[113] In accordance with some embodiments of the invention, the endogenous RNA molecule may be one or more endogenous microRNA molecules, for example, one or more of the miR156 and/or miR172 RNA molecules. The sequences of the endogenous miR156 and miR172 molecules will depend on the specific plant species in which the FT transgene will be expressed. The FT transgene can be designed to encode a target site or mRNA sensor for a miR156 or miR172 molecule in a plant species of interest, which can be selected based on the level and timing of expression of one or more miR156 and miR172 molecules. In soybean, there are three miR172 molecules that are expressed at higher levels during the transition from vegetative to generative stage, miRNA172a (SEQ ID NO: 95), miRNA172c (SEQ ID NO: 96), or miRNA172k (SEQ ID NO: 97), and there are three miR156 molecules that are present in excess at the transition from young to mature plant, miR156a (SEQ ID NO: 103), miR156c (SEQ ID NO: 104), or miR156q (SEQ ID NO: 105). Thus, a polynucleotide sequence encoding an FT protein may further comprise a sequence encoding one or more target sites or sensors for one or more such endogenous miR156 or miR172 RNA molecules. Each of the target sites or sensors of miR156 or miR172 can be located in the pre-mRNA and/or mature mRNA transcript encoded by the FT transgene, for example, within the coding, 5'UTR, 3'UTR and/or intron RNA sequence encoded by the transgene's polynucleotide sequence. FT, although miR156 or miR172 target sites or sensors are more often in a non-coding and/or non-translated sequence. Examples of sequences encoding a target site or sensor for the soybean miR156 molecule include SEQ ID NOs: 106, 108, 109 and 110, which are complementary to one or more miR156 molecules. For example, the sequence given as SEQ ID NO: 106 encodes SEQ ID NO: 107 as the target site or mRNA sensor for miR156. Examples of sequences encoding a target site or sensor for the soybean miR172 molecule include SEQ ID NOs: 98, 100 and 101, which are complementary to one or more miR172 molecules. For example, the sequence given as SEQ ID NO: 98 encodes SEQ ID NO: 99 as the target site or mRNA sensor for miR172. In this case, the sequence of the target site or sensor of the FT transgene can be determined based on the complementary sequence of the known miR156 or miR172 molecule. Indeed, depending on the sequence of one or more of the endogenous miR156, miR172, and/or other small RNA molecules naturally occurring in a given plant species, the transgenic FT expression cassette transformed into that plant species can be engineered to encode a target site or sensor for such an endogenous miR156, miR172 or other small RNA molecule. In accordance with some embodiments of the invention, the first expression cassette containing the FT transgene can be designed to have a sequence encoding a target site or sensor for an endogenous miR156, miR172, or other small RNA molecule, even if in the same recombinant DNA molecule, construct or vector, or in the same transgenic plant, a second expression cassette is present that contains a DNA sequence suitable for transcription encoding an RNA molecule that additionally targets the same FT transgene for suppression.

[114] In accordance with embodiments of the present invention, the target site for an endogenous RNA molecule will depend on the plant in which the FT transgene will be expressed. The miR156 and miR172 sequences (and their target sites and sensors) are known for many dicotyledonous species. The target site or sensor of the transgenic FT mRNA complementary to the endogenous RNA molecule may be 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, or 27 (or more) nucleotides in length. Typically, the target site or sensor of the FT transgene is designed to be 100% complementary to endogenous miR156 or miR172. However, the target site or sensor for an endogenous suppressor RNA molecule does not need to be 100% complementary to endogenous miR156 or miR172 to be effective (i.e., to hybridize to miR156 or miR172 and be a target for suppression). For example, not entirely perfect complementarity may allow more than one miR156, miR172, and/or other endogenous RNA molecule to hybridize to a target site or sensor. For any given plant species, the target site or sensor encoded by the coding polynucleotide sequence of the FT transgene may vary to some extent, yet bind or hybridize to an endogenous RNA molecule, such as an endogenous miR156 or miR172 RNA molecule, when expressed in a plant cell. Accordingly, the target site of an mRNA transcript encoded by the FT transgene may contain one or more mismatches, e.g. , miR156, miR172) and mRNA transcriptome. Indeed, the target site encoded by the coding polynucleotide sequence of the FT transgene may be at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least at least 99.5%, at least 99.9%, or 100% complementary to an endogenous RNA molecule targeting sequence (e.g., miR156 or miR172), such as the target site or sensor for the a miR172 molecule in soy (SEQ ID NO: 98, 100 or 101) target site or sensor for the a miR156 molecule in soybean (SEQ ID NO: 106, 108, 109 or 110).

[115] According to another broad aspect of the present invention, methods are provided for transforming a cell, tissue, or plant explant with a recombinant DNA molecule, construct, or vector provided herein to produce a transgenic plant. The recombinant DNA molecule, construct or vector may contain an FT transgene or an expression cassette. Depending on the type of suppression, the FT transgene may additionally contain a target site for an endogenous RNA molecule. The recombinant DNA molecule, construct, or vector may contain a second expression cassette containing a DNA sequence suitable for transcription encoding an RNA molecule that targets the FT transgene for suppression. Alternatively, the FT transgene and a transcriptionable DNA sequence encoding an RNA molecule can be in two separate DNA molecules, constructs or vectors that can be transformed into plants together or separately. A recombinant DNA molecule, construct or vector containing the FT transgene and a transcriptionable DNA sequence encoding an RNA molecule that targets the FT transgene for suppression can be transformed into a plant. In accordance with other embodiments of the invention, a first recombinant DNA molecule, construct or vector containing the FT transgene, and a second recombinant DNA molecule, construct or vector containing a DNA sequence suitable for transcription encoding an RNA molecule that targets the FT transgene for suppression can be co-transform into a plant. In accordance with other embodiments of the invention, a plant transformed with a first expression cassette containing the FT transgene can be transformed with a second expression cassette containing a DNA sequence suitable for transcription encoding an RNA molecule that targets the FT transgene for suppression, or a plant transformed with the first expression cassette , containing a DNA sequence suitable for transcription, encoding an RNA molecule that targets the FT transgene for suppression, can be transformed with a second expression cassette containing the FT transgene. In accordance with additional embodiments of the invention, a first transgenic plant containing a first expression cassette containing (i) an FT transgene or (ii) a DNA sequence suitable for transcription encoding an RNA molecule that targets the FT transgene for suppression can be crossed with a second plant, containing a second expression cassette containing (i) a DNA sequence suitable for transcription encoding an RNA molecule that targets the FT transgene for suppression, or (ii) the FT transgene, so that one or more daughter plants can be obtained containing both the first, and a second expression cassette (i.e. both the FT transgene and a DNA sequence suitable for transcription).

[116] Numerous methods are known in the art for transforming chromosomes in plant cells with a recombinant DNA molecule, construct or vector, which can be used in accordance with the methods of this invention to obtain a transgenic plant cell, plant part and plant. In accordance with the methods presented, any suitable methods or techniques for transforming plant cells known in the art can be used. effective plant transformation methods include bacterial-mediated transformation such as Agrobacterium -mediated or Rhizhobium -mediated transformation and microparticle bombardment-mediated transformation. Various methods are known in the art for transforming explants with a transformation vector by bacterial-mediated transformation or microparticle bombardment and then culturing etc. of these explants to regenerate or grow transgenic plants. Other methods of plant transformation such as microinjection, electroporation, vacuum infiltration, high pressure application, ultrasound, mixing with silicon carbide fibers, PEG-mediated transformation, etc., are also known in the art. Transgenic plants produced by these transformation methods may be chimeric or non-chimeric with respect to the transformation event, depending on the methods and explants used. Suitable methods for plastid transformation with a recombinant DNA molecule or construct are also known in the art.

[117] Further provided are methods for expressing an FT transgene in one or more plant cells or tissues under the control of a vegetative stage promoter, which can also be a meristem-preferred or meristem-specific promoter. Expression of the FT transgene can be modified, attenuated and/or adjusted by having a target site or sensor for an endogenous RNA molecule in the mRNA encoded by the FT transgene such that the endogenous RNA molecule is targeted to the FT transgene for suppression. In addition to the FT transgene, an RNA molecule that targets the FT transgene for suppression can also be expressed from a transcriptionable DNA sequence transformed into a plant. Such methods can be used to change the flowering time of a plant and/or the number of productive or successful flowers, fruits, pods and/or seeds per plant node compared to a wild-type plant or control plant lacking the FT transgene. Indeed, the methods of this invention can be used to alter the generative or yield related phenotypes or traits of a transgenic plant.

[118] the Transformation of the target plant material or explant can be practiced in tissue culture in a nutrient medium, for example, a mixture of nutrients that promote cell growth in vitro. Target recipient cells or explants may include, but are not limited to, meristems, shoot tips, protoplasts, hypocotyls, callus, immature or mature embryos, shoots, buds, nodal sections, leaves, gametes such as microspores, pollen, sperm, eggs , etc. or any suitable parts thereof. It is contemplated that any transformable cell or tissue that can be regenerated or grown into a fertile plant can be used as a target for transformation. Transformed explants, cells or tissues can be subjected to additional culture steps such as callus induction, selection, regeneration, etc., as is known in the art. Transformed cells, tissues, or explants containing the recombinant DNA insert can be grown or regenerated into transgenic plants in culture, media, or soil, according to methods known in the art. Transgenic plants can be further crossed with each other or with other plants to produce transgenic seeds and offspring. A transgenic plant can also be obtained by crossing a first plant containing the recombinant DNA sequence or transformation event with a second plant lacking the insert. For example, the recombinant DNA sequence can be introduced into a first line of plants to be transformed, which can then be crossed with a second line of plants to introgress the recombinant DNA sequence into a second plant line. The progeny from such a cross can be further backcrossed multiple times with more desirable lines, e.g. over 6-8 backcross generations, to produce progeny with essentially the same genotype as the original parent line, but with a recombinant DNA sequence introduced.

[119] The recombinant DNA construct or expression cassette of the invention can be incorporated into a DNA transformation vector for use in transforming a target cell, tissue, or plant explant. Such a transformation vector according to the invention may generally contain sequences or elements necessary or useful for efficient transformation, in addition to a transcriptional DNA sequence and/or an FT transgene or expression cassette. In the case of Agrobacterium -mediated transformation, the transformation vector may contain a segment of engineered transfer DNA (or T-DNA) or a region having two boundary sequences, a left border (LB) and a right border (RG), flanking at least a DNA sequence suitable for transcription and /or an FT transgene, such that insertion of the T-DNA into the plant genome will create a transformation event for a DNA sequence suitable for transcription and/or an FT transgene. In other words, a DNA sequence suitable for transcription and/or an FT transgene would be located between the left and right borders of the T-DNA, possibly along with additional transgenes or expression cassettes such as a plant selectable marker transgene and/or other genes of agronomic interest, which may provide a trait or phenotype of agronomic interest to a plant. In addition to protein coding sequences, genes of agronomic interest may further comprise a polynucleotide sequence encoding a suppressor RNA element. In some embodiments, the transcriptional DNA sequence and/or the FT transgene and the plant selectable marker transgene (or other gene of agronomic interest) may reside in separate T-DNA segments in the same or different recombinant DNA molecules, such as for joint transformation. The transformation vector or construct may further comprise prokaryotic support elements which, in the case of Agrobacterium -mediated transformation, may reside in the backbone of the vector outside of the T-DNA region(s).

[120] In accordance with some embodiments of the invention, the first expression cassette containing the FT transgene and the second expression cassette containing a DNA sequence suitable for transcription encoding an RNA molecule that targets the FT transgene for suppression may be in the same T-DNA of the transformational vector (i.e., between the same right and left T-DNA borders); the first expression cassette containing the FT transgene may be in the first T-DNA (containing the first right border and the first left border), and the second expression cassette containing a DNA sequence suitable for transcription encoding an RNA molecule that targets the FT transgene for suppression, may be in the second T-DNA (containing the second right border and the second left border), while the first and second T-DNA are in the same transformation vector; or the first expression cassette containing the FT transgene may be in the first T-DNA of the first transformation vector, and the second expression cassette containing a DNA sequence suitable for transcription encoding an RNA molecule that targets the FT transgene for suppression may be in the second T- DNA of the second transformation vector. The first and second expression cassettes in one or two transformation vectors can be co-transformed into a plant cell, or the first and second expression cassettes can be in two separate transformation vectors and are transformed into plant cells separately. The first and second expression cassettes can be transformed into one or more plant cells already containing a transformation event for another expression cassette, or the first and second expression cassettes can be transformed into different plant cells that can be grown or regenerated into the first and second transgenic plant. The first or second transgenic plants and/or their progeny can be crossed with each other so as to bring together the first and second expression cassettes in one plant.

[121] A plant selectable marker transgene in a transformation vector or construct of the present invention can be used to aid in the selection of transformed cells or tissues due to the presence of a selection agent, such as an antibiotic or herbicide, wherein the plant selection marker transgene provides resistance or resistance to the selection agent. Thus, the selection agent can support or promote the survival, development, growth, proliferation, etc. of transformed cells expressing the plant selection marker gene, so as to increase the proportion of transformed cells in the plant R ₀ . Commonly used plant selectable marker genes include, for example, those conferring resistance or resistance to antibiotics such as kanamycin or paromomycin ( nptII ), hygromycin B ( aph IV ), streptomycin or spectinomycin ( aadA ) and gentamicin ( aac3 and aacC4 ), or those that provide resistance or resistance to herbicides such as glufosinate ( bar or pat ), dicamba ( DMO ), and glyphosate ( aroA or EPSPS ). It is also possible to use plant selectable marker genes that allow visual screening for transformants, for example, luciferase or green fluorescent protein (GFP), or a beta-glucuronidase expressing gene, or the uidA (GUS) gene, for which various chromogenic substrates are known.

[122] According to embodiments of the present invention, methods for transforming a cell, tissue, or plant explant with a recombinant DNA molecule or construct may further comprise site-directed or targeted integration. According to these methods, a portion of the recombinant donor template DNA molecule (ie, the sequence to be inserted) can be inserted or integrated at a desired site or locus in the plant genome. The sequence for inserting the donor template may comprise a transgene or construct, such as (i) an FT transgene or a construct containing a polynucleotide sequence encoding an FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter, and /or (ii) a DNA sequence suitable for transcription encoding an RNA molecule that targets the FT transgene for suppression and is operably linked to a vegetative stage promoter and/or a generative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. The donor template may also have one or two homology arms flanking the insertion sequence to promote a targeted insertion event via homologous recombination and/or homologous repair. Thus, the recombinant DNA molecule of the invention may further comprise a donor template for site-directed or targeted integration of a transgene or construct, such as a transgene or FT construct, into the plant genome.

[123] Any site or locus in the plant genome can potentially be selected for site-directed integration of a transgene or construct of the invention. In the case of site-directed integration, a double-strand break or “nick” can first be created at the selected genomic locus with a site-specific nuclease such as, for example, zinc finger nuclease (ZFN), meganuclease, transcription activator-like nuclease (TALEN), Argonaute (non-limiting examples of Argonaute proteins include Thermus thermophilus Argonaute (TtAgo), Pyrococcus furiosus Argonaute (PfAgo), Natronobacterium gregoryi Argonaute (NgAgo), RNA-directed nuclease (non-limiting examples of RNA-directed nucleases include Cas1, Cas1B, Cas2, Cas3, Cas4, Cas5, Cas6, Cas7, Cas8, Cas9 (also known as Csn1 and Csx12), Cas10, Csy1, Csy2, Csy3, Cse1, Cse2, Csc1, Csc2, Csa5, Csn2, Csm2, Csm3, Csm4, Csm5, Csm6, Cmr1, Cmr3, Cmr4, Cmr5, Cmr6, Csb1, Csb2, Csb3, Csx17, Csx14, Csx10, Csx16, CsaX, Csx3, Csx1, Csx15, Csf1, Csf2, Csf3, Csf4, Cpf1, their homologues or their modified versions); recombinases ( without limitation, for example, tyrosine recombinase attached to m DNA recognition motif ( eg , Cre recombinase, Flp recombinase, Tnp1 recombinase), serine recombinase attached to DNA recognition motif ( eg, PhiC31 integrase, R4 integrase, TP-901 integrase); transposase (without limitation, for example , DNA transposase attached to a DNA-binding domain); or any combination of them. Guide RNAs ( eg, CRISPR RNA (crRNA), trans -activating CRISPR RNA (tracrRNA), guide RNA (nRNA), single guide RNA (sRNA)) useful in methods using RNA-targeted nucleases are also provided. Any method known in the art for site-directed integration can be used. In the presence of a donor template molecule, repair of a double-strand break or "nick" can occur via homologous recombination between the homology arms of the donor template and the plant genome, or via non-homologous end joining (NGSK), leading to site-directed integration of the insert sequence into the plant genome to create a targeted insertion event at or near the site of the double-strand break or "nick". Thus, site-specific insertion or integration of a transgene or construct can be achieved.

[124] As used herein, the term "insert" in relation to plant transformation or site-directed integration refers to the insertion or integration of an exogenous polynucleotide or DNA construct, molecule, or sequence, such as a transformation vector or T-DNA sequence, or a donor template insert sequence. , into the plant genome. In this context, the term "exogenous" refers to a polynucleotide or DNA construct, molecule or sequence that is introduced into a plant cell or tissue using a suitable plant transformation or genome editing method or method known in the art.

[125] In accordance with embodiments of the present invention, a plant that can be transformed with a recombinant DNA molecule or a transformation vector containing an FT transgene and/or a DNA sequence suitable for transcription encoding an RNA molecule that targets the FT transgene for suppression may include a plurality of flowering plants or angiosperms, which can be further defined as including various types of dicotyledonous (dicotyledonous) plants, such as soybean, cotton, alfalfa, canola, sugar beet, alfalfa and other leguminous plants. The dicotyledonous plant may be a representative of the species Brassica sp. (e.g. B. napus, B. rapa, B. juncea ), in particular Brassica species, used as a source of vegetable oils, alfalfa ( Medicago sativa ), sunflower ( Helianthus annuus ), safflower ( Carthamus tinctorius ), oil palm ( Elaeis spp.), sesame ( Sesamum spp.), coconut ( Cocos spp.), soybean ( Glycine max ), tobacco ( Nictiana tabacum ), potato ( Solanum tuberosum ), peanut ( Arachis hypogaea ), cotton ( Gossypium barbadense, Gossypium hirsutum ), sweet potato ( Ipomoea batatus ), cassava ( Manihot esculenta ), coffee ( Coffea spp.), tea ( Camellia spp.), fruit trees such as apple ( Malus spp.), Prunus spp. such as plum, apricot , peach, cherry, etc., pear ( Pyrus spp.), fig ( Ficus casica ), banana ( Musa spp.), etc., citrus trees ( Citrus spp.), coconut ( Theobroma cacao ), avocado ( Persea americana ), olive ( Olea europaea ), almond ( Prunus amygdalus ), walnut ( Juglans spp.), strawberry ( Fragaria s pp.), watermelon ( Citrullus lanatus ), pepper ( Capsicum spp.), sugar beet ( Beta vulgaris ), grapes (Vitis, Muscadinia ), tomato (Lycopersicon esculentum, Solanum lycopersicum ) and cucumber ( Cucumis sativis ). Leguminous plants include beans and peas. Legumes include, for example, guar, carob, fenugreek, soybeans, common beans, cowpeas, mung beans, lima beans, common beans, lentils and chickpeas. Given that this invention may apply to a wide range of plant species, this invention additionally relates to other botanical structures similar to beans of legumes, such as seed pods, pods, fruits, nuts, tubers, etc.

[126] In accordance with embodiments of the present invention and depending on the specific plant species being transformed, a plant with ectopic expression of a florigenic FT sequence that can be modified by suppression as proposed herein may have an altered or greater number of pods, pods, fruits, nuts, tubers, etc. per node(s), main stem and/or branch(s) of the plant, and/or modified or more pods, pods, fruits, nuts, tubers, etc. per plant compared to a wild-type plant or a control plant that does not contain the FT transgene.

[127] According to another broad aspect of the present invention, transgenic plants, plant cells, seeds, and plant parts are provided, comprising one or more transformation events or one or more inserts in the genome of at least one plant cell, wherein the transformation event or insert comprises (i) a recombinant DNA sequence, construct, or polynucleotide comprising a Flowering Locus T (FT) transgene, wherein the FT transgene contains a polynucleotide sequence encoding an FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter and/or (ii) a DNA sequence suitable for transcription encoding an RNA molecule that targets the FT transgene for suppression, operably linked to a vegetative stage promoter and/or a generative stage promoter, which may also be a meristem-preferred or meristem-specific promoter . As proposed herein, the FT transgene can be targeted for suppression by a transgene-expressed and/or endogenous RNA molecule. An RNA molecule can be encoded by a transcriptionable DNA sequence that is also transformed into a plant, plant part, plant seed, or plant cell. The FT protein encoded by the polynucleotide sequence may correspond to the native FT gene in the transgenic plant transformed with the polynucleotide coding sequence, or be homologous or similar to the FT protein native to the transgenic plant (i.e., not native to the transgenic plant, but similar to native or endogenous FT protein), or heterologous for the transgenic plant. Such a transgenic plant can be obtained by any convenient transformation method, which can be followed by selection, cultivation, regeneration, development, etc., if desired or necessary to obtain a transgenic R ₀ plant, which can then be self-pollinated or crossed with other plants to create seeds. R1 and subsequent generations and seeds through additional crossing, etc. Similarly, embodiments of this invention further include a cell, tissue, explant, etc. plants containing one or more transgenic cells having a transformation event or genomic insertion of recombinant DNA or a polynucleotide sequence containing the FT transgene and/or a DNA sequence suitable for transcription encoding an RNA molecule that targets the FT transgene for suppression.

[128] The transgenic plants, plant cells, seeds, and plant parts of the invention may be homozygous or hemizygous for a transgenic event or insertion of an FT transgene and/or a DNA sequence suitable for transcription into the genome of at least one plant cell, or may contain any the number of copies of transgenic events or inserts containing the FT transgene and/or a DNA sequence suitable for transcription. The dose or amount of expression of the FT transgene may vary depending on its zygosity and/or copy number, which may affect the degree or extent of phenotypic changes in the transgenic plant, etc. In accordance with some embodiments of the invention, a transgenic plant containing an FT transgene and /or an FT suppression element that can be modified, attenuated, and/or adjusted as provided herein, may further be characterized as having one or more altered phenotypes or traits associated with flowering or generativity, which may include altered yield-related traits, or phenotypes such as an increase in the number of flowers, pods, etc. and/or seeds per plant (and/or plant node) compared to a wild-type plant or control plant that does not contain the FT transgene (and/or does not contain a suppression element F.T.). Such a transgenic plant may be further characterized as having an altered structure, morphology and/or architecture due to a change in plant height, branching profile, number of flower nodes, etc., compared to a wild-type or control plant. In fact, yield-related phenotypes or traits altered by overexpression of FT in a transgenic plant may include: flowering time, length of generative stage, length of flowering, number or time of falling of flowers, pods, pods, pods, fruits, nuts, etc. ., number of flowers per node, number of racemes per node, number of branches per plant, number of nodes per plant, number of nodes on main stem, number of nodes on branches, number of pods, pods, pods, fruits, nuts, etc. on plant, number of beans, pods, pods, fruits, nuts, etc. per node, number of beans, pods, pods, fruits, nuts, etc. per main stem, number of pods, seeds, pods, pods , fruit, nuts, etc. on twigs, seed weight (e.g., 1000 seed weight), number of seeds per plant, number of seeds per main stem, number of seeds per node, and/or altered plant architecture compared to wild-type plant or control p asthenia not containing the FT transgene (and/or not containing an FT suppressor element). In the context of this document, the term "overexpression" in relation to the FT transgene includes ectopic expression of the transgene.

[129] For the purposes of this invention, a "plant" may include an explant, seedling, sprout, or whole plant at any stage of regeneration or development. As used herein, the term "transgenic plant" refers to a plant whose genome has been altered by integration or insertion of a recombinant DNA molecule, construct or sequence. A transgenic plant includes an R ₀ plant grown or regenerated from its original transformed plant cell(s), as well as progeny of the transgenic plants in subsequent generations or crosses of the transgenic R ₀ plant. In the context of this document, the term "plant part" may refer to any organ or intact tissue of a plant, such as the meristem, shoot organ/structure (e.g., leaf, stem, and tuber), root, flower, or flower organ/structure (e.g., bract, sepal, petal, stamen, carpel, anther and ovule), seed (e.g. embryo, endosperm and seed coat), fruit (e.g. mature ovary), propagule, or other plant tissues (e.g. vascular tissue, integumentary tissue, etc.) .), or any part of them. Parts of the plant according to the present invention may be viable, non-viable, regenerable and/or non-regenerate. "Propagula" may include any part of a plant that is capable of growing into a whole plant. For the purposes of this invention, a plant cell transformed with an FT transgene and/or an FT suppression element according to embodiments of this invention may include any plant cell that is competent for transformation as known in the art, given the transformation method, such as meristem cell, embryonic cell, callus cell, etc. As used herein, the term "transgenic plant cell" simply refers to any plant cell transformed with a stably integrated recombinant DNA molecule or sequence. The transgenic plant cell may include an original transformed plant cell, a transgenic plant cell of a regenerated or grown plant R ₀ , or a transgenic plant cell from any daughter plant of the transformed R ₀ plant, including seed or plant embryo cells, or cultured plant cells or callus cells, etc. d.

[130] In accordance with many embodiments of the invention, the transgenic plant may contain a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter. In accordance with some embodiments of the invention, the expression of the florigenic FT protein in a transgenic plant can be suppressed at the vegetative and/or generative stage and/or in the vegetative and/or generative tissue of the transgenic plant, for example, by means of an endogenous and/or transgenic or ectopically expressed RNA molecule. In accordance with some embodiments of the invention, expression of the florigenic FT protein in a transgenic plant may be spatially and/or temporally restricted by a small RNA molecule. In accordance with some embodiments of the invention, the transgenic plant may contain a transcriptional DNA sequence encoding an RNA molecule that targets the florigenic gene or FT transgene for suppression.

[131] Embodiments of the present invention may further include methods for creating or obtaining transgenic plants having altered generative and/or yield-related traits or phenotypes, for example, by transformation, crossing, etc., the method comprising introducing a recombinant DNA molecule , construct or sequence containing the FT transgene and/or a DNA sequence suitable for transcription encoding an RNA molecule that targets the FT transgene for suppression into a plant cell, and then regeneration or cultivation of the transgenic plant from the transformed plant cell, which can be carried out under conditions selection pressure favorable for a transgenic event. Such methods may include transforming a plant cell with a recombinant DNA molecule or sequence containing an FT transgene and/or a DNA sequence suitable for transcription, and selecting a plant having one or more altered phenotypes or traits, such as one or more of the following: flowering time, duration generative stage, duration of flowering, number or time of fall of flowers, pods, pods, pods, fruits, nuts, etc., number of flowers per node, number of racemes per node, number of branches per plant, number of nodes per plant, number of nodes on main stem, number of nodes on branches, number of beans, pods, pods, fruits, nuts, etc. per plant, number of beans, pods, pods, fruit, nuts, etc. per node, number of beans, pods, pods, fruits, nuts, etc. per main stem, number of pods, seeds, pods, pods, fruits, nuts, etc. per twig, weight of seeds (e.g. weight of 1000 seeds ), number of seeds per plant, number of seeds per main stem, number of seeds per node, and/or altered plant architecture, compared to a wild-type plant or control plant lacking the FT transgene (and/or lacking an FT suppression element). For example, embodiments of the invention may include methods for producing a transgenic plant having an increased number of flowers, pods, and/or seeds per plant (and/or an increased number of flowers, pods, and/or seeds per plant node), the method comprising introducing a recombinant molecule DNA containing the FT transgene and/or a DNA sequence suitable for transcription into a plant cell, and then regenerating or growing the transgenic plant from the plant cell. The transgenic plant can then be selected based on one or more of the above generative and/or yield related traits or phenotypes. Selection of a transgenic plant, plant cell, or plant tissue can also be made based on the presence of the FT transgene and/or FT suppression element using one or more methods or kits known in the art, such as DNA sequencing, hybridization, antibody binding, and/or other molecular technologies.

[132] According to embodiments of the invention, the transgenic plant may have at least 1%, at least 2%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least at least 60%, at least 70%, at least 75%, at least 80%, at least 90%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 250%, at least 300%, at least 350% or at least 400% more flowers, beans, seeds, pods, pods, fruits, nuts or tubers than a non-transgenic control plant. In accordance with some embodiments of the invention, the transgenic plant may have an average of at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 60%, at least 70%, at least 75%, at least 80%, at least 90%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 250%, at least 300%, at least 350% or at least 400% more flowers, beans, seeds, pods, pods, fruits, nuts or tubers per node, than the non-transgenic control plant. The transgenic plant may have, on average, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, or at least 10 beans, pods, pods, fruits, nuts or tubers per node. A transgenic plant may average 2-10, 2-9, 2-8, 2-7, 2-6, 2-5, 2-4, 2-3, 3-10, 3-9, 3-8, 3-7, 3-6, 3-5, 3-4, 4-10, 4-9, 4-8, 4-7, 4-6 or 4-5 beans, pods, pods, fruits, nuts or tubers per knot. The transgenic plant may, on average, have at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least at least 8, at least 9, or at least 10 more flowers, pods, seeds, pods, pods, fruits, nuts, or tubers per node compared to the non-transgenic control plant. The transgenic plant may be a soybean plant, and the transgenic plant may, on average, have more nodes and/or seeds per node than a non-transgenic control plant. The transgenic plant can flower for at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 20, at least 25, at least 30, at least 35, at least 40, or at least 45 days earlier than the non-transgenic control plant.

[133] According to embodiments of the present invention, a transgenic plant is provided, comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, wherein the expression of the polynucleotide coding sequence is spatially and/or temporally attenuated, restricted, modified and/or corrected. an RNA molecule, which may be a small non-coding RNA molecule. The level of expression or translation of the florigenic FT mRNA and/or protein in the transgenic plant may be suppressed or reduced in one or more meristematic, generative and/or floral tissues and/or during one or more generative steps. In accordance with some embodiments of the invention, the duration of the generative stage and/or the duration of flowering of a transgenic plant containing an FT transgene and/or an FT suppression element proposed herein may be at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11 , at least 12, at least 13, at least 14, at least 15, at least 20, at least 25, at least 30, at least 35, at least 40 or at least 45 days longer than the duration of the generative stage and/or the duration of flowering of the wild-type plant or control plant, but may also be no more than (i.e. no more than) 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 25, 30, 35, 40, 45, 50, 55 or 60 days longer, h we eat the duration of the generative stage and/or the duration of flowering of the wild-type plant or control plant. In accordance with some embodiments of the invention, the onset of flowering (i.e., the appearance of the first open flower) of a transgenic plant containing an FT transgene and / or an FT suppression element proposed herein can be or occur at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10 , at least 11, at least 12, at least 13, at least 14, at least 15, at least 20, at least 25, at least 30, at least 35, at least 40, or at least 45 days earlier than the wild-type plant or control plant, but may also be no more than (i.e., no more than) 3, 4, 5 , 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 25, 30, 35, 40, 45, 50, 55, or 60 days earlier than the start of flowering of the wild-type plant or by control plant.

[134] In accordance with some embodiments of the invention, the number of flowers, seeds, pods, pods, fruits, nuts, beans, or tubers in a transgenic plant containing an FT transgene and/or an FT suppression element provided herein may be at least at least 1%, at least 2%, at least 3%, at least 4%, at least 5%, at least 6%, at least 7%, at least 8%, at least 9%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 60%, at least 70%, at least 75%, at least 80 %, at least 90%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 250% , at least 300%, according to me at least 350% or at least 400% more than the wild-type plant or control plant. In accordance with some embodiments of the invention, the number of flowers, seeds, pods, pods, fruits, nuts, or beans on the main stem of a transgenic plant containing an FT transgene and/or an FT suppression element provided herein may be at least 1 %, at least 2%, at least 3%, at least 4%, at least 5%, at least 6%, at least 7%, at least 8% at least 9%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 60%, at least 70%, at least 75%, at least 80%, at least 90%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 250%, at least measure by 300%, by m at least 350% or at least 400% more than wild-type plant or control plant. In accordance with some embodiments of the invention, the number of flowers, seeds, pods, pods, fruits, nuts, or beans per node on the main stem of a transgenic plant containing an FT transgene and/or an FT suppression element provided herein may be at least 1%, at least 2%, at least 3%, at least 4%, at least 5%, at least 6%, at least 7%, at least 8%, at least 9%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35 %, at least 40%, at least 45%, at least 50%, at least 60%, at least 70%, at least 75%, at least 80% at least 90%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 250%, at least 30 0%, at least 350% or at least 400% more than the wild-type plant or control plant.

[135] Each of these characteristic numbers or numerical values per plant, main stem or branch, such as the number of flowers, pods, seeds, pods, fruits, nuts or beans per plant, main stem or branch, can be calculated as the average of two or more plants or determined for a single plant, and the numbers or numbers of flowers, pods, seeds, pods, fruits, nuts or beans per node can be calculated as an average of one or more plants. Thus, it is possible to calculate the percentage change between two plants, between a plant and the average of two or more plants, or between two averages (with each detrimental value taken from two or more plants).

[136] In accordance with some embodiments of the invention, the number of flowers, pods, seeds, pods, fruits, nuts, beans, or tubers per node of a transgenic plant containing an FT transgene and/or an FT suppression element provided herein may, on average, be at least 1%, at least 2%, at least 3%, at least 4%, at least 5%, at least 6%, at least 7%, at least 8%, at least 9%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 60%, at least 70%, at least 75%, at least at least 80%, at least 90%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least by 250% at least e. 300%, at least 350%, or at least 400% more than the wild-type plant or control plant. In accordance with some embodiments of the invention, a transgenic plant containing an FT transgene and/or an FT suppression element provided herein may have an average of at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9 or at least 10 flowers, pods, pods, seeds, pods, fruits, nuts, beans or tubers per node. In accordance with some embodiments of the invention, a transgenic plant containing an FT transgene and/or an FT suppression element provided herein may have an average of 2-10, 2-9, 2-8, 2-7, 2-6, 2 -5, 2-4, 2-3, 3-10, 3-9, 3-8, 3-7, 3-6, 3-5, 4-10, 4-9, 4-8, 4-7 , 4-6 or 4-5, or about 2, about 3, about 4, about 5, about 6, about 7, about 8, about 9 or about 10 flowers, pods, pods, fruits, nuts, beans or tubers to the node. In accordance with some embodiments of the invention, a transgenic plant containing an FT transgene and/or an FT suppression element provided herein may have an average of at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9 or at least 10 more flowers, pods, seeds, pods, fruits, nuts, beans or tubers per node compared to a non-transgenic wild-type plant or control plant.

[137] In accordance with some embodiments of the invention, the average number of pods, pods, seeds, fruits, nuts, beans, or tubers and/or the average number of flowers, pods, pods, seeds, fruits, nuts, beans, or tubers per node of the transgenic plants containing the FT transgene and/or the FT suppression element provided herein (or on the main stem of such a transgenic plant) can be 1%-400%, 1%-350%, 1%-300%, 1%- 250%, 1%-200%, 1%-150%, 1%-100%, 1%-75%, 1%-50%, 1%-25%, 5%-400%, 5%-350% , 5%-300%, 5%-250%, 5%-200%, 5%-150%, 5%-100%, 5%-95%, 5%-90%, 5%-85%, 5 %-80%, 5%-75%, 5%-70%, 5%-65%, 5%-60%, 5%-55%, 5%-50%, 5%-45%, 5%- 40%, 5%-35%, 5%-30%, 5%-25%, 5%-20%, 5%-15%, 5%-10%, 10%-400%, 10%-350% , 10%-300%, 10%-250%,10%-200%, 10%-150%, 10%-100%, 10%-90%, 10%-80%, 10%-70%, 10 %-60%, 10%-50%, 10%-40%, 10%-30%, 10%-20%, 25%-400%, 25%-350%, 25%-300%, 25%- 250%, 25%-200%, 25%-150%, 25%-100%, 25%-75%, 25%-50%, 50%-400%, 50%-350%, 50%-300% , 50%-250% , 50%-200%, 50%-150%, 50%-100%, 50%-75%, 75%-400%, 75%-350%, 75%-300%, 75%-250%, 75 %-200%, 75%-150%, 75%-100%, 100%-400%, 100%-350%, 100%-300%, 100%-250%, 100%-200% or 100%- 150% more than wild-type plant or control plant. In accordance with some embodiments of the invention, the duration of the generative stage of a transgenic plant containing an FT transgene and/or an FT suppression element proposed herein can be 1%-400%, 1%-350%, 1%-300%, 1 %-250%, 1%-200%, 1%-150%, 1%-100%, 1%-75%, 1%-50%, 1%-25%, 5%-400%, 5%- 350%, 5%-300%, 5%-250%, 5%-200%, 5%-150%, 5%-100%, 5%-95%, 5%-90%, 5%-85% , 5%-80%, 5%-75%, 5%-70%, 5%-65%, 5%-60%, 5%-55%, 5%-50%, 5%-45%, 5 %-40%, 5%-35%, 5%-30%, 5%-25%, 5%-20%, 5%-15%, 5%-10%, 10%-400%, 10%- 350%, 10%-300%, 10%-250%, 10%-200%, 10%-150%, 10%-100%, 10%-90%, 10%-80%, 10%-70% , 10%-60%, 10%-50%, 10%-40%, 10%-30%, 10%-20%, 25%-400%, 25%-350%, 25%-300%, 25 %-250%, 25%-200%, 25%-150%, 25%-100%, 25%-75%, 25%-50%, 50%-400%, 50%-350%, 50%- 300%, 50%-250%, 50%-200%, 50%-150%, 50%-100%, 50%-75%, 75%-400%, 75%-350%, 75%-300% , 75%-250%, 75%-200%, 75%-150%, 75%-100%, 100%-400%, 100%-350%, 100%-300%, 100%-250%, 100 %-200% or 100%-150% more, in terms of number of days, than growth wild type or control plant. In accordance with some embodiments of the invention, the onset of flowering of a transgenic plant containing an FT transgene and/or an FT suppression element provided herein may be 1%-400%, 1%-350%, 1%-300%, 1%. -250%, 1%-200%, 1%-150%, 1%-100%, 1%-75%, 1%-50%, 1%-25%, 5%-400%, 5%-350 %, 5%-300%, 5%-250%, 5%-200%, 5%-150%, 5%-100%, 5%-95%, 5%-90%, 5%-85%, 5%-80%, 5%-75%, 5%-70%, 5%-65%, 5%-60%, 5%-55%, 5%-50%, 5%-45%, 5% -40%, 5%-35%, 5%-30%, 5%-25%, 5%-20%, 5%-15%, 5%-10%, 10%-400%, 10%-350 %, 10%-300%, 10%-250%, 10%-200%, 10%-150%, 10%-100%, 10%-90%, 10%-80%, 10%-70%, 10%-60%, 10%-50%, 10%-40%, 10%-30%, 10%-20%, 25%-400%, 25%-350%, 25%-300%, 25% -250%, 25%-200%, 25%-150%, 25%-100%, 25%-75%, 25%-50%, 50%-400%, 50%-350%, 50%-300 %, 50%-250%, 50%-200%, 50%-150%, 50%-100%, 50%-75%, 75%-400%, 75%-350%, 75%-300%, 75%-250%, 75%-200%, 75%-150%, 75%-100%, 100%-400%, 100%-350%, 100%-300%, 100%-250%, 100% -200% or 100%-150% earlier, in terms of number of days, than the wild-type plant or control plant.

[138] In accordance with embodiments of the present invention, the transgenic plants provided herein may have a combination of two or more of the traits or phenotypes described herein, for example, two or more traits from an increased number of beans, pods, pods, seeds, fruits , nuts or tubers per node, increased number of pods, pods, pods, seeds, fruits, nuts or tubers per main stem, increased duration of the generative stage, earlier onset of flowering, minimum plant height such as at least 900 millimeters or more (i.e., greater than or equal to 0.9 meters) in the case of soybean, and/or reduced branching compared to the wild-type plant or control plant. In accordance with some embodiments of the invention, transgenic plants may, on average, have an increased number of pods, pods, pods, seeds, fruits, or nuts per node, or an earlier onset of flowering compared to a wild-type or control plant. Such transgenic plants may additionally have an increased number of pods, pods, pods, seeds, fruits, nuts, or tubers per stem and/or an increased duration of the generative stage compared to a wild-type or control plant.

[139] For example, the transgenic plant provided herein may have at least 1%, at least 2%, at least 5%, at least 10%, at least 15%, at least at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 60%, at least 70%, at least 75%, at least 80%, at least 90%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 250%, at least 300%, at least 350% or at least 400% more flowers, beans, seeds , pods, pods, fruits, nuts or tubers per node and the beginning of flowering, which occurs at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least at least 20, at least 25, at least 30, at least 35, at least 40, or at least 45 days earlier than the wild-type or control plant. The transgenic plant provided herein can have an average of at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, or at least 10 more flowers, pods, seeds, pods, pods, fruits, nuts, or tubers per node, and an onset of flowering that occurs at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 20, at least 25, at least 30, at least 35, at least 40, or at least 45 days earlier than the wild-type plant or control plant. Such transgenic plants may further have a generative stage and/or flowering duration that is at least 1, at least 2, at least 3, at least 4, at least 5, at least 6 , at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 20, at least 25, at least 30, at least 35, at least 40, or at least 45 days longer than the duration of the generative stage and/or flowering of a wild-type plant or control plant, and/or an increased number of flowers, pods, seeds, pods, pods, fruits or nuts on the main stem of the transgenic plant, which is at least 1%, at least 2%, at least at least 3%, at least 4%, at least 5%, at least 6%, at least 7%, at least 8%, at least 9%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30 %, at least 35%, at least 40%, at least 45%, at least 50%, at least 60%, at least 70%, at least 75% at least 80%, at least 90%, at least 100%, at least 125%, at least 150%, at least 175%, or at least 200%, at least 250%, at least 300%, at least 350%, or at least 400% more than the wild-type plant or control plant.

[140] In accordance with embodiments of the present invention, the transgenic plants provided herein may have an altered plant architecture with minimal plant height and reduced branching, which may be accompanied by a slight decrease in the number of nodes per plant. Transgenic plants expressing only the FT transgene can have a pronounced dwarf phenotype due to early termination, which implies a small plant height along with a reduction in branching and the number of nodes per plant. By expressing the FT transgene with a microRNA sensor or a second suppressor element targeting the FT transgene, these pronounced early termination phenotypes can be attenuated to produce more normal plant height while maintaining an increased number of pods per node. In accordance with these embodiments of the invention, transgenic plants may have (i) a minimum plant height that is reduced by no more than 5%, 10%, 15%, 20%, 25%, 30%, 35%, or 40% compared to wild-type plant or control plant (i.e., the difference in plant height between the transgenic plant and the wild-type plant or control plant does not exceed one or more of these percentages), (ii) total number of branches reduced by at least 50% at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, by at least 90% or at least 95% compared to the wild-type plant or control plant, and/or (iii) a minimum number of nodes per plant (and/or main stem) that is reduced by no more than 5 %, 10%, 15%, 20%, 25%, 30%, 35%, or 40% compared to wild-type plant or control plant (i.e., difference in number number of nodes per plant (and/or main stem) between the transgenic plant and the wild-type plant or control plant does not exceed one or more of these percentages). In the case of soybean, the transgenic plant provided herein may have a minimum height that is at least 700 millimeters (at least), at least 750 at least at least 800 at least at least 850 at least at least 900 at least at least 950 at least at least 1000 at least at least 1050 at least at least 1100 at least at least 1150 at least at least 1200 at least at least 1250 at least, at least 1300 at least, at least 1350 at least or at least 1400 at least; a total number of nodes per plant that is at least 100, at least 125, at least 150, at least 175, at least 200, at least 225, at least 250, at least 275, at least at least 300, at least 325, at least 350, at least 375, or at least 400 nodes; and/or a total number of branches that is reduced by at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, or at least 95% compared to the wild-type plant or control plant. All of the above numerical, percentage, and difference values for a plant further include values calculated from the average of multiple plants of the same type.

[141] In accordance with another broad aspect of the present invention, methods are provided for planting transgenic plants of the present invention at normal or high density in a field. In accordance with some embodiments of the invention, crop yield per acre (or land area) can be increased by planting the transgenic plants of the invention at higher field densities. As described herein, transgenic plants according to the invention expressing the florigen FT protein during vegetative stage(s) of development and/or with suppression of the FT transgene may exhibit an increased number of pods and/or seeds per node (in particularly on the main stem), but may also have altered plant architecture with reduced branching and fewer nodes per branch. Therefore, it has been suggested that the transgenic plants of this invention can be planted at higher densities to increase yield per acre in the field. For row crops, higher density can be achieved by planting more seeds/plants per row and/or by reducing row spacing. In accordance with some embodiments of the invention, a transgenic crop plant according to this invention can be planted at a density in the field (number of plants per land/field area) that is at least 5%, 10%, 15%, 20%, 25%, 50 %, 75%, 100%, 125%, 150%, 175%, 200%, 225%, or 250% higher than or greater than the normal planting density for such a crop in accordance with standard agricultural practice.

[142] In the case of soybean, typical planting density is in the range of about 100,000 to 150,000 seeds per acre, and typical row spacing is in the range of about 26 to about 40 inches, for example, row spacing is 30 inches or 36 inches. Within a given row, they can typically plant about 6-8 soybean seeds per foot. In contrast, a high density planting for soybeans may include a range of approximately 150,000 to 250,000 seeds per acre, and row spacing may range from about 10 inches or less to about 25 inches, for example, row spacing is 10 inches. , 15 inches or 20 inches. When planting at high density, approximately 9-12 soybean seeds per foot can be sown within each row, possibly in combination with smaller row spacing. At the same time, a high density of crops can be provided by a narrow distance between rows without increasing the sowing density within each row.

[143] In the case of cotton, typical planting density is in the range of about 28,000 to 45,000 seeds per acre, and typical row spacing is in the range of about 38 to about 40 inches, for example, row spacing is 38 inches or 40 inches. Within a given row, they can typically plant about 2-3 cottonseeds per foot. In contrast, high density planting for cotton can include a range of about 48,000 to 60,000 seeds per acre, and row spacing can range from about 30 inches or less to about 36 inches. When planting at high density, approximately 3-5 cotton seeds per foot can be sown within each row, possibly in combination with less row spacing. At the same time, a high crop density in the case of cotton can be provided by a narrow distance between rows without increasing the sowing density within each row.

[144] In the case of canola, typical planting density ranges from about 360,000 to 550,000 seeds per acre, and typical row spacing (between cultivator tines) ranges from about 6 inches to about 16 inches. Within a given row, they can typically plant about 8-12 canola seeds per foot. In contrast, high density planting for canola can include a range of about 450,000 to 680,000 seeds per acre, and row spacing can range from about 5 inches or less to about 10 inches. When planting at high density, approximately 10-16 canola seeds per foot can be planted within each row, possibly in combination with less row spacing. At the same time, a high crop density in the case of canola can be provided by a narrow distance between the rows without increasing the planting density within each row.

[145] The following are non-limiting exemplary embodiments of the present invention:

1. A recombinant DNA construct comprising a first expression cassette and a second expression cassette, wherein the first expression cassette contains a polynucleotide sequence encoding the florigenic FT protein operably linked to a first promoter suitable for expression in plants, and the second expression cassette contains a DNA sequence suitable for transcription , encoding an RNA molecule containing a targeting sequence that is at least 80% complementary to at least 15 consecutive nucleotides of the polynucleotide sequence of the first expression cassette, and wherein a DNA sequence suitable for transcription is operably linked to a second promoter suitable for expression in plants.

2. The recombinant DNA construct of Embodiment 1, wherein the targeting sequence of the RNA molecule is about 15 to about 27 nucleotides in length.

3. Recombinant DNA construct according to embodiment 1 or 2, characterized in that the targeting sequence of the RNA molecule is 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, or 27 nucleotides long .

4. Recombinant DNA construct according to any one of embodiments of the invention 1-3, characterized in that the targeting sequence of the RNA molecule is at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% complementary to at least 15 consecutive nucleotides of the polynucleotide sequence of the first expression cassette.

5. A recombinant DNA construct according to any of the embodiments of the invention 1-4, characterized in that the targeting sequence of the RNA molecule is at least 80% complementary to at least 15 consecutive nucleotides of the mRNA transcript encoded by the polynucleotide sequence of the first expression cassette.

6. Recombinant DNA construct according to any one of embodiments 1-5, characterized in that the targeting sequence of the RNA molecule is at least 80% complementary to at least 15 consecutive nucleotides of the exon or coding sequence of the mRNA transcript.

7. Recombinant DNA construct according to any one of embodiments 1-5, characterized in that the targeting sequence of the RNA molecule is at least 80% complementary to at least 15 consecutive nucleotides of the non-coding sequence of the mRNA transcript.

8. Recombinant DNA construct according to any one of the embodiments of the invention 1-7, characterized in that the RNA molecule encoded by a DNA sequence suitable for transcription is a microRNA or miRNA precursor.

9. Recombinant DNA construct according to any one of embodiments of the invention 1-8, characterized in that the DNA sequence suitable for transcription contains a sequence that is at least 80%, at least 85%, at least 90% , at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% complementary to SEQ ID NO: 68 or 69.

10. Recombinant DNA construct according to any one of embodiments of the invention 1-9, characterized in that the DNA sequence suitable for transcription contains a sequence that is at least 80%, at least 85%, at least 90% , at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% identical to SEQ ID NO: 65.

11. Recombinant DNA construct according to any one of embodiments of the invention 1-10, characterized in that the polynucleotide sequence of the first expression cassette contains a sequence that is at least 80%, at least 85%, at least 90% , at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% identical to SEQ ID NO: 69.

12. Recombinant DNA construct according to any of the embodiments of the invention 1-11, characterized in that the FT florigen protein contains an amino acid sequence having at least 60%, at least 65%, at least 70%, at least 75 %, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity with a sequence selected from the group consisting of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14 , 16, 18, 20, 22, 24, 26, 28 and 30, or a functional fragment thereof.

13. Recombinant DNA construct according to embodiment 12, characterized in that the florigen FT protein additionally contains one or more of the following amino acids: tyrosine or another uncharged polar or non-polar residue at the amino acid position of the florigen FT protein corresponding to amino acid position 85 in SEQ ID NO: 14; leucine or other non-polar residue at the amino acid position of the FT florigenic protein corresponding to amino acid position 128 in SEQ ID NO: 14; and tryptophan or other large non-polar residue at the amino acid position of the FT florigenic protein corresponding to amino acid position 138 of SEQ ID NO: 14.

14. Recombinant DNA construct according to embodiment 12, characterized in that the FT florigen protein does not contain one or more of the following amino acids: histidine at the amino acid position corresponding to lysine or arginine at the amino acid position corresponding to position 85 in SEQ ID NO: 14 ; lysine or arginine at the amino acid position corresponding to position 128 in SEQ ID NO: 14; and serine, aspartic acid, glutamic acid, lysine or arginine at the amino acid position corresponding to position 138 in SEQ ID NO: 14.

15. Recombinant DNA construct according to embodiment 12, characterized in that the FT florigen protein contains an amino acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18 , 20, 22, 24, 26, 28 and 30, or a functional fragment thereof.

16. Recombinant DNA construct according to any of embodiments of the invention 1-15, characterized in that the polynucleotide sequence has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96% , at least 97%, at least 98%, or at least 99% identity with a sequence selected from the group consisting of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27 and 29.

17. A recombinant DNA construct according to any one of embodiments 1-16, wherein the first promoter suitable for expression in plants is a vegetative stage promoter.

18. A recombinant DNA construct according to any one of embodiments 1-17, wherein the first promoter suitable for expression in plants is a meristem-preferred or meristem-specific promoter.

19. A recombinant DNA construct according to any of the embodiments of the invention 1-18, characterized in that the first promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45 or 48, or a functional part thereof.

20. Recombinant DNA construct according to embodiment 19, characterized in that the first promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5% or 100% identical to SEQ ID NO: 31, or its functional part.

21. Recombinant DNA construct according to embodiment 19, characterized in that the first promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NO: 32 or SEQ ID NO :48.

22. Recombinant DNA construct according to embodiment 19, characterized in that the first promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5% or 100% identical to SEQ ID NO: 44, or its functional part.

23. A recombinant DNA construct according to any one of the embodiments of the invention 1-22, characterized in that the first promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63 or 64, or a functional part thereof.

24. Recombinant DNA construct according to embodiment 23, characterized in that the first promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80% at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NO: 49, or its functional part.

25. Recombinant DNA construct according to any one of embodiments of the invention 1-24, characterized in that the second promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63 or 64, or a functional part thereof.

26. A recombinant DNA construct according to any one of embodiments of the invention 1-25, characterized in that the second promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NO: 49 , or its functional part.

27. Recombinant DNA construct according to any one of embodiments of the invention 1-26, characterized in that the second promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% identical to a polynucleotide sequence selected from group consisting of SEQ ID NO: 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93 or 94, or a functional part thereof.

28. A recombinant DNA construct according to any one of the embodiments of the invention 1-27, characterized in that the first promoter suitable for expression in plants is a vegetative stage promoter, and the second promoter suitable for expression in plants is a late vegetative stage promoter and/or a generative stage promoter. stages.

29. Recombinant DNA construct according to embodiment 28, characterized in that the first promoter suitable for expression in plants is an early vegetative stage promoter.

30. The recombinant DNA construct of embodiment 28, wherein the second promoter suitable for expression in plants is the preferred generative stage promoter.

31. A recombinant DNA construct according to any one of the embodiments of the invention 1-30, characterized in that the first promoter suitable for expression in plants initiates detectable expression of the polynucleotide sequence encoding the florigenic FT protein at an earlier stage of development than that at which the second promoter suitable for expression in plants initiates detectable expression of a DNA sequence suitable for transcription.

32. A DNA molecule or vector containing a recombinant DNA construct according to any one of embodiments of the invention 1-31.

33. A plasmid vector for Agrobacterium -mediated transformation containing a recombinant DNA construct according to any one of the embodiments of the invention 1-31.

34. Donor template molecule for site-mediated integration, containing a recombinant DNA construct according to any of embodiments of the invention 1-31.

35. A transgenic plant comprising an insertion of a recombinant DNA construct according to any one of embodiments 1-31 in the genome of at least one cell of the transgenic plant.

36. The transgenic plant of embodiment 35, wherein the transgenic plant is homozygous for the insertion of the recombinant DNA construct.

37. The transgenic plant according to embodiment 35, wherein the transgenic plant is hemizygous for the insertion of the recombinant DNA construct.

38. A transgenic plant according to any one of the embodiments of the invention 35-37, characterized in that the transgenic plant is a short day plant.

39. A transgenic plant according to any one of the embodiments of the invention 35-38, characterized in that the transgenic plant is a dicotyledonous plant.

40. A transgenic plant according to any one of embodiments 35-39, wherein the transgenic plant is a leguminous plant.

41. A transgenic plant according to any one of embodiments 35-40, wherein the transgenic plant is a soybean.

42. The transgenic plant of embodiment 41, wherein the transgenic soybean plant produces more pods per node than a control plant that does not contain the recombinant DNA construct.

43. The transgenic plant of any one of embodiments 35-42, wherein the transgenic plant produces more flowers per node than a control plant that does not contain the recombinant DNA construct.

44. A transgenic plant or part thereof according to any one of embodiments 35-43, wherein the transgenic plant produces more seeds, pods, pods, fruits, nuts, or beans per node of the transgenic plant than a control plant that does not contain recombinant DNA - design.

45. A transgenic plant or part thereof according to any one of the embodiments of the invention 35-44, characterized in that the transgenic plant blooms earlier than a control plant that does not contain a recombinant DNA construct.

46. A transgenic plant or part thereof according to any one of embodiments 35-45, wherein the transgenic plant has more flower clusters per node than a control plant that does not contain the recombinant DNA construct.

47. A part of a transgenic plant containing an insertion of a recombinant DNA construct according to any one of the embodiments of the invention 1-31 in the genome of at least one cell of the transgenic plant part.

48. A transgenic plant part according to any of embodiments 44-47, characterized in that the transgenic plant part is one of the following: seed, fruit, leaf, cotyledon, hypocotyl, meristem, embryo, endosperm, root, shoot, stem, bean, flower, inflorescence, pedicel, stalk, pistil, spiracle, receptacle, petal, sepal, pollen, stamen, filament, ovary, ovule, pericarp, phloem, or vascular tissue.

49. Recombinant DNA construct containing a polynucleotide sequence encoding the florigenic FT protein operably linked to a promoter suitable for expression in plants, wherein the polynucleotide sequence contains a sequence that encodes a target site in the mRNA transcript encoded by the polynucleotide sequence, and at the same time the target site The mRNA transcript is at least 80% complementary to the endogenous RNA molecule.

50. Recombinant DNA construct according to embodiment 49, characterized in that the target site of the mRNA transcript is at least 17 nucleotides long.

51. Recombinant DNA construct according to embodiment 49 or 50, characterized in that the target site of the mRNA transcript is 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, or 27 nucleotides long.

52. Recombinant DNA construct according to any of the embodiments of the invention 49-51, characterized in that the target site of the mRNA transcript is at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5% or but 100% complementary to the endogenous RNA molecule.

53. Recombinant DNA construct according to any of the embodiments of the invention 49-52, characterized in that the target site is located in the non-coding sequence of the mRNA transcript.

54. Recombinant DNA construct according to any of the embodiments of the invention 49-53, characterized in that the target site of the mRNA transcript is at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% complementary to SEQ ID NOs: 95, 96, 97, 103, 104, or 105.

55. Recombinant DNA construct according to any of the embodiments of the invention 49-54, characterized in that the target site of the mRNA transcript is at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% complementary to SEQ ID NO: 95 or 103.

56. The recombinant DNA construct according to any of the embodiments of the invention 49-55, characterized in that the polynucleotide sequence encoding the florigenic FT protein contains a sequence that is at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% identical to SEQ ID NOs: 98, 99 , 100, 101, 106, 107, 108, 109 or 110.

57. A recombinant DNA construct according to any of the embodiments of the invention 49-54 or 56, characterized in that the target site of the mRNA transcript is at least 80%, at least 85%, at least 90%, according to at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% identical to SEQ ID NO: 99 or 107.

58. Recombinant DNA construct according to any of the embodiments of the invention 49-57, characterized in that the FT florigen protein contains an amino acid sequence having at least 60%, at least 65%, at least 70%, at least 75 %, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity with a sequence selected from the group consisting of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14 , 16, 18, 20, 22, 24, 26, 28 and 30, or a functional fragment thereof.

59. Recombinant DNA construct according to any of the embodiments of the invention 49-58, characterized in that the florigenic FT protein additionally contains one or more of the following amino acids: tyrosine or other uncharged polar or non-polar residue at the amino acid position of the florigenic FT protein corresponding to the amino acid position 85 in SEQ ID NO: 14; leucine or other non-polar residue at the amino acid position of the FT florigenic protein corresponding to amino acid position 128 in SEQ ID NO: 14; and tryptophan or other large non-polar residue at the amino acid position of the FT florigenic protein corresponding to amino acid position 138 of SEQ ID NO: 14.

60. Recombinant DNA construct according to any of the embodiments of the invention 49-58, characterized in that the FT florigen protein does not contain one or more of the following amino acids: histidine at the amino acid position corresponding to lysine or arginine at the amino acid position corresponding to position 85 in SEQ ID NO: 14; lysine or arginine at the amino acid position corresponding to position 128 in SEQ ID NO: 14; and serine, aspartic acid, glutamic acid, lysine or arginine at the amino acid position corresponding to position 138 in SEQ ID NO: 14.

61. Recombinant DNA construct according to any of the embodiments of the invention 49-61, characterized in that the florigenic FT protein contains an amino acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14 , 16, 18, 20, 22, 24, 26, 28 and 30, or a functional fragment thereof.

62. Recombinant DNA construct according to any of the embodiments of the invention 49-62, characterized in that the polynucleotide sequence has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96% , at least 97%, at least 98%, or at least 99% identity with a sequence selected from the group consisting of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27 and 29.

63. Recombinant DNA construct according to any one of the embodiments of the invention 49-62, characterized in that the promoter suitable for expression in plants is a vegetative stage promoter.

64. Recombinant DNA construct according to any one of the embodiments of the invention 49-63, characterized in that the promoter suitable for expression in plants is a meristem-preferred or meristem-specific promoter.

65. A recombinant DNA construct according to any of the embodiments of the invention 49-64, characterized in that the promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95 %, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5% or 100% identical with a polynucleotide sequence selected from the group , consisting of SEQ ID NO: 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45 or 48, or a functional part thereof.

66. A recombinant DNA construct according to any of the embodiments of the invention 49-65, characterized in that the promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95 %, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NO: 31, or its functional part.

67. A recombinant DNA construct according to any of the embodiments of the invention 49-65, characterized in that the promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95 %, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NO: 32 or SEQ ID NO: 48.

68. A recombinant DNA construct according to any of the embodiments of the invention 49-65, characterized in that the promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95 %, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NO: 44, or its functional part.

69. A recombinant DNA construct according to any of the embodiments of the invention 49-68, characterized in that the promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95 %, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5% or 100% identical with a polynucleotide sequence selected from the group , consisting of SEQ ID NO: 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63 or 64, or a functional part thereof.

70. A DNA molecule or vector containing a recombinant DNA construct according to any of the embodiments of the invention 49-69.

71. Plasmid vector for Agrobacterium -mediated transformation containing a recombinant DNA construct according to any of the embodiments of the invention 49-69.

72. Donor template molecule for site-mediated integration, containing a recombinant DNA construct according to any of the embodiments of the invention 49-69.

73. A transgenic plant comprising an insertion of a recombinant DNA construct according to any one of embodiments 49-69 in the genome of at least one cell of the transgenic plant.

74. The transgenic plant according to embodiment 73, wherein the transgenic plant is homozygous for the insertion of the recombinant DNA construct.

75. The transgenic plant according to embodiment 73, wherein the transgenic plant is hemizygous for the insertion of the recombinant DNA construct.

76. A transgenic plant according to any one of the embodiments of the invention 73-75, characterized in that the transgenic plant is a short day plant.

77. A transgenic plant according to any one of the embodiments of the invention 73-76, characterized in that the transgenic plant is a dicotyledonous plant.

78. A transgenic plant according to any one of embodiments 73-77, wherein the transgenic plant is a leguminous plant.

79. A transgenic plant according to any one of the embodiments of the invention 73-78, characterized in that the transgenic plant is a soybean.

80. The transgenic plant of any one of embodiments 73-79, wherein the transgenic soybean plant produces more beans per node than a control plant that does not contain the recombinant DNA construct.

81. The transgenic plant of any one of embodiments 73-80, wherein the transgenic plant produces more flowers per node than a control plant that does not contain the recombinant DNA construct.

82. A transgenic plant or part thereof according to any one of embodiments 73-81, wherein the transgenic plant produces more pods, pods, fruits, nuts, or beans per node of the transgenic plant than a control plant that does not contain the recombinant DNA construct .

83. A transgenic plant or part thereof according to any one of the embodiments of the invention 73-82, characterized in that the transgenic plant blooms earlier than a control plant that does not contain a recombinant DNA construct.

84. The transgenic plant, or part thereof, of any one of embodiments 73-83, wherein the transgenic plant has more flower clusters per node than a control plant that does not contain the recombinant DNA construct.

85. A part of a transgenic plant containing an insertion of a recombinant DNA construct according to any one of the embodiments of the invention 49-69 in the genome of at least one cell of the transgenic plant part.

86. A transgenic plant part according to any one of the embodiments of the invention 82-85, characterized in that the transgenic plant part is one of the following: seed, fruit, leaf, cotyledon, hypocotyl, meristem, embryo, endosperm, root, shoot, stem, bean, flower, inflorescence, pedicel, stalk, pistil, spiracle, receptacle, petal, sepal, pollen, stamen, filament, ovary, ovule, pericarp, phloem, or vascular tissue.

87. Recombinant DNA construct containing a DNA sequence suitable for transcription, encoding an RNA molecule containing a targeting sequence that is at least 80% complementary to at least 15 consecutive nucleotides of a polynucleotide sequence encoding a florigen FT protein, moreover, a DNA sequence suitable for transcription is operably linked to a promoter suitable for expression in plants.

88. The recombinant DNA construct of embodiment 87, wherein the targeting sequence of the RNA molecule is about 15 to about 27 nucleotides in length.

89. Recombinant DNA construct according to embodiment 87 or 88, characterized in that the targeting sequence of the RNA molecule is at least 85%, at least 90%, at least 95%, at least 96% , at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% complementary to at least 15 consecutive nucleotides of the polynucleotide sequence encoding the florigenic FT protein.

90. Recombinant DNA construct according to any of the embodiments of the invention 87-89, characterized in that the targeting sequence of the RNA molecule is at least 80% complementary to at least 15 consecutive nucleotides of the mRNA transcript encoded by the polynucleotide sequence encoding the florigen FT protein .

91. A recombinant DNA construct according to any one of the embodiments of the invention 87-90, characterized in that the targeting sequence of the RNA molecule is at least 80% complementary to at least 15 consecutive nucleotides of the exon or coding sequence of the mRNA transcript.

92. A recombinant DNA construct according to any one of the embodiments of the invention 87-91, characterized in that the targeting sequence of the RNA molecule is at least 80% complementary to at least 15 consecutive nucleotides of the non-coding sequence of the mRNA transcript.

93. A recombinant DNA construct according to any of the embodiments of the invention 87-92, characterized in that the RNA molecule encoded by a DNA sequence suitable for transcription is a microRNA or miRNA precursor.

94. Recombinant DNA construct according to any of the embodiments of the invention 87-93, characterized in that the florigenic FT protein encoded by the polynucleotide sequence contains an amino acid sequence having at least 60%, at least 65%, at least 70% at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity with a sequence selected from the group consisting of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 and 30, or a functional fragment thereof.

95. Recombinant DNA construct according to any of the embodiments of the invention 87-94, characterized in that the florigenic FT protein additionally contains one or more of the following amino acids: tyrosine or another uncharged polar or non-polar residue at the amino acid position of the florigenic FT protein corresponding to the amino acid position 85 in SEQ ID NO: 14; leucine or other non-polar residue at the amino acid position of the FT florigenic protein corresponding to amino acid position 128 in SEQ ID NO: 14; and tryptophan or other large non-polar residue at the amino acid position of the FT florigenic protein corresponding to amino acid position 138 of SEQ ID NO: 14.

96. Recombinant DNA construct according to any of the embodiments of the invention 87-94, characterized in that the FT florigen protein does not contain one or more of the following amino acids: histidine at the amino acid position corresponding to lysine or arginine at the amino acid position corresponding to position 85 in SEQ ID NO: 14; lysine or arginine at the amino acid position corresponding to position 128 in SEQ ID NO: 14; and serine, aspartic acid, glutamic acid, lysine or arginine at the amino acid position corresponding to position 138 in SEQ ID NO: 14.

97. Recombinant DNA construct according to any of the embodiments of the invention 87-96, characterized in that the FT florigen protein contains an amino acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14 , 16, 18, 20, 22, 24, 26, 28 and 30, or a functional fragment thereof.

98. Recombinant DNA construct according to any of the embodiments of the invention 87-97, characterized in that the polynucleotide sequence encoding the florigenic FT protein has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95% , at least 96%, at least 97%, at least 98%, or at least 99% identity with a sequence selected from the group consisting of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27 and 29.

99. A recombinant DNA construct according to any of the embodiments of the invention 87-98, characterized in that the promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95 %, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5% or 100% identical with a polynucleotide sequence selected from the group , consisting of SEQ ID NO: 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63 or 64, or a functional part thereof.

100. A recombinant DNA construct according to any of the embodiments of the invention 87-99, characterized in that the promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95 %, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NO: 49, or its functional part.

101. Recombinant DNA construct according to any of the embodiments of the invention 87-100, characterized in that the promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95 %, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5% or 100% identical with a polynucleotide sequence selected from the group , consisting of SEQ ID NO: 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91 , 92, 93 or 94, or a functional part thereof.

102. Recombinant DNA construct according to any of the embodiments of the invention 87-101, characterized in that the promoter suitable for expression in plants is a vegetative stage promoter.

103. Recombinant DNA construct according to any one of the embodiments of the invention 87-102, characterized in that the promoter suitable for expression in plants is a late vegetative stage promoter and/or a generative stage promoter.

104. Recombinant DNA construct according to any of the embodiments of the invention 87-103, characterized in that the promoter suitable for expression in plants is heterologous with respect to the DNA sequence suitable for transcription.

105. A DNA molecule or vector containing a recombinant DNA construct according to any of the embodiments of the invention 87-104.

106. A transgenic plant comprising an insertion of a recombinant DNA construct according to any one of the embodiments of the invention 87-104 in the genome of at least one cell of the transgenic plant.

107. The transgenic plant of embodiment 106, wherein the transgenic plant is a short day plant.

108. The transgenic plant of embodiment 106 or 107, wherein the transgenic plant is a dicotyledonous plant.

109. A transgenic plant according to any one of embodiments 106-108, wherein the transgenic plant is a leguminous plant.

110. A transgenic plant according to any one of the embodiments of the invention 106-109, characterized in that the transgenic plant is a soybean.

111. A part of a transgenic plant containing an insertion of a recombinant DNA construct according to any of the embodiments of the invention 87-103 in the genome of at least one cell of the transgenic plant part.

112. A transgenic plant part according to embodiment 111, characterized in that the transgenic plant part is one of the following: seed, fruit, leaf, cotyledon, hypocotyl, meristem, embryo, endosperm, root, shoot, stem, bean, flower, inflorescence, pedicel, stalk, pistil, spiracle, receptacle, petal, sepal, pollen, stamen, filament, ovary, ovule, pericarp, phloem, or vascular tissue.

113. A method for producing a transgenic plant, including

(a) transforming at least one explant cell with a recombinant DNA construct according to any one of embodiments 1-31, 49-69, or 87-104; and

(b) regenerating or growing the transgenic plant from the transformed explant.

114. The method of embodiment 113, further comprising:

(c) selecting a transgenic plant having one or more of the following traits or phenotypes: earlier flowering, longer generative or flowering period, increased number of flowers per node, increased number of flower clusters per node, increased number of pods, pods, pods , fruits or nuts per node and an increased number of seeds per node compared to a control plant not containing the recombinant DNA construct.

115. The method of embodiment 113 or 114, wherein the transformation step (a) is carried out by Agrobacterium -mediated transformation or microparticle bombardment of the explant.

116. The method according to any of the embodiments of the invention 113-115, characterized in that the step of transformation (a) includes site-directed integration of the recombinant DNA construct.

117. A method for planting a transgenic crop plant, including:

planting a transgenic crop plant at an increased density in a field, wherein the transgenic crop plant contains an insert of a recombinant DNA construct according to any one of embodiments 1-31, 49-69, or 87-104.

118. The method of embodiment 117 wherein the transgenic crop plant is a soybean and about 150,000 to 250,000 seeds of the transgenic soybean plant are planted per acre.

119. The method of embodiment 117 wherein the transgenic crop is cotton and about 48,000 to 60,000 seeds of the transgenic cotton plant are planted per acre.

120. The method of embodiment 117 wherein the transgenic crop plant is canola and about 450,000 to 680,000 seeds of the transgenic canola plant are planted per acre.

121. The transgenic plant according to any of the embodiments of the invention 35-46, 73-84 or 106-110, characterized in that the transgenic plant has at least 1%, at least 2%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 60%, at least 70%, at least 75%, at least 80%, at least 90%, at least at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 250%, at least 300%, at least 350% or at least 400% more seeds, pods, pods, pods, fruits, nuts or tubers than the non-transgenic control plant.

122. The transgenic plant according to any of the embodiments of the invention 35-46, 73-84 or 106-110, characterized in that the transgenic plant has an average of at least 5%, at least 10%, at least 15 %, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50% at least 60%, at least 70%, at least 75%, at least 80%, at least 90%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 250%, at least 300%, at least 350% or at least 400% more seeds , beans, pods, pods, fruits, nuts or tubers per node than the non-transgenic control plant.

123. The transgenic plant according to any of the embodiments of the invention 35-46, 73-84 or 106-110, characterized in that the transgenic plant has an average of at least 2, at least 3, at least 4, at least 5 , at least 6, at least 7, at least 8, at least 9 or at least 10 beans, pods, pods, fruits, nuts or tubers per node.

124. The transgenic plant according to any of the embodiments of the invention 35-46, 73-84 or 106-110, characterized in that the transgenic plant has an average of 2-10, 2-9, 2-8, 2-7, 2-6 , 2-5, 2-4, 3-10, 3-9, 3-8, 3-7, 3-6, 3-5, 4-10, 4-9, 4-8, 4-7, 4 -6 or 4-5 beans, pods, pods, fruits, nuts or tubers per node.

125. The transgenic plant according to any of the embodiments of the invention 35-46, 73-84 or 106-110, characterized in that the transgenic plant has an average of at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, or at least 10 more pods, pods, fruits, nuts, or beans per node compared to a non-transgenic control plant.

126. A transgenic plant according to any one of embodiments of the invention 35-46, 73-84 or 106-110, characterized in that the transgenic plant blooms at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12 , at least 13, at least 14, at least 15, at least 20, at least 25, at least 30, at least 35, at least 40, or at least 45 days earlier than the non-transgenic control plant.

127. The transgenic plant of any one of embodiments 121, 122, 123, 124, 125, or 126, wherein the transgenic plant is a soybean plant and the transgenic plant has, on average, more beans per node than the non-transgenic control plant.

128. A transgenic plant containing a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, wherein the expression of the florigenic FT protein is suppressed in the late vegetative and/or generative tissue.

129. A transgenic plant according to embodiment 128, characterized in that the expression of the florigenic FT protein is suppressed by a small RNA molecule.

130. Recombinant DNA construct containing a polynucleotide sequence encoding florigen FT protein and functionally linked to a vegetative stage promoter, and at least one DNA sequence suitable for transcription, encoding an RNA molecule containing a targeting sequence that is complementary to at least part of the polynucleotide sequences.

131. A transgenic plant containing an insert of a recombinant DNA construct according to embodiment 130 in the genome of at least one cell of the transgenic plant.

132. A transgenic plant containing a recombinant polynucleotide sequence encoding the florigenic FT protein operably linked to a vegetative stage promoter, wherein the expression of the polynucleotide sequence is spatially and temporally limited by a small RNA molecule.

133. A recombinant DNA construct containing an expression cassette, wherein the expression cassette contains a polynucleotide sequence encoding the florigenic FT protein operably linked to a promoter suitable for expression in plants, while the promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93 %, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% identical to the polynucleotide sequence, selected from the group consisting of SEQ ID NO: 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63 or 64, or a functional part thereof.

134. Recombinant DNA construct according to embodiment 130 or 133, characterized in that the FT florigen protein contains an amino acid sequence having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least at least 96%, at least 97%, at least 98%, or at least 99% identity with a sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16 , 18, 20, 22, 24, 26, 28 and 30, or a functional fragment thereof.

135. A DNA molecule or vector containing a recombinant DNA construct according to embodiment 130 or 133.

136. A plasmid vector for Agrobacterium -mediated transformation containing a recombinant DNA construct according to embodiment 130 or 133.

137. Donor template molecule for site-mediated integration containing a recombinant DNA construct according to embodiment 130 or 133.

138. A transgenic plant containing an insertion of a recombinant DNA construct according to embodiment 130 or 133 in the genome of at least one cell of the transgenic plant.

139. A transgenic plant according to any one of embodiments 131, 132, or 138, wherein the transgenic plant is homozygous for the insertion of the recombinant DNA construct.

140. A transgenic plant according to any one of embodiments 131, 132, 138, or 139, wherein the transgenic plant is hemizygous for the insertion of the recombinant DNA construct.

141. The transgenic plant of any one of embodiments 131, 132, or 138-140, wherein the transgenic plant is a short day plant.

142. A transgenic plant according to any one of embodiments 131, 132, or 138-141, wherein the transgenic plant is a dicotyledonous plant.

143. A transgenic plant according to any one of embodiments 131, 132, or 138-142, wherein the transgenic plant is a leguminous plant.

144. The transgenic plant of any one of embodiments 131, 132, or 138-143, wherein the transgenic plant is soybean.

145. The transgenic plant of any one of embodiments 35-46, 73-84, 106-110, 131, 132, or 138-144, wherein the transgenic plant produces more seeds, pods, pods, fruits, nuts, or beans per node of the transgenic plant than a control plant that does not contain the recombinant DNA construct.

146. A transgenic plant according to any one of embodiments 35-46, 73-84, 106-110, 131, 132, or 138-145, characterized in that the transgenic plant blooms earlier than a control plant that does not contain the recombinant DNA construct.

147. The transgenic plant of any one of embodiments 35-46, 73-84, 106-110, 131, 132, or 138-146, wherein the transgenic plant is a soybean plant that has a height of at least about 700 millimeters, at stage R8.

148. The transgenic plant of any one of embodiments 35-46, 73-84, 106-110, 131, 132, or 138-147, wherein the transgenic plant is a soybean plant that has at least 100 nodes per plant at stage R8.

149. Part of a transgenic plant containing an insert of a recombinant DNA construct according to embodiment 130 or 133 in the genome of at least one cell of the transgenic plant part.

EXAMPLES

Example 1 Short Day Induction Conditions for Soybeans and Identification of Flowering Locus T (FT) Genes by Transcription Profiling.

[146] This example was previously described in co-pending US Patent Application No. 15/131987 and International Application No. PCT/US2016/028130, which are incorporated herein by reference in their entirety. Methods for photoperiodic light processing (i.e.short day conditions) are described in US Pat. No. 8,935,880 and US Patent Application Publication No. 2014/0259905, which are incorporated herein by reference in their entirety. As further described therein, early induction under short day conditions produced soybean plants having altered generative traits, including an increased number of pods/seeds per plant. Transcription profiling experiments were performed using expression microarrays to determine the presence of upregulation for a particular transcript in these light-induced plants in order to identify genes that may be responsible for mediating short day induction phenotypes. In these experiments, transcripts were analyzed for soybean leaf and flower top tissues harvested from plants 1, 3 and 5 days after they were placed under inductive conditions of short daylight hours (short day), in comparison with plant tissues that were not placed under inductive conditions. (a long day)

[147] As illustrated in FIG. 2, increased transcript accumulation was observed for a specific Flowering Locus T gene, Gm.FT2a (SEQ ID NO: 1), in leaves harvested 3 and 5 days after application of early short day induction (ESD) conditions, compared to samples obtained from (i) flower top tissues of the same plants subjected to short day induction, (ii) leaf and flower top tissues of soybean plants that were placed under long day conditions instead. These data support the conclusion that Gm.FT2a expression is induced in leaf tissues of plants subjected to rICD conditions, which was not observed in plants grown under long day conditions. Also, Gm.FT2a expression was not observed in the flower tops of rICD-treated plants, consistent with a pattern of FT protein expression that is induced in peripheral leaf tissues in response to inductive photoperiodic conditions and then migrates to its site of action in the meristems to induce flowering. However, additional experiments using a more sensitive RNA sequencing analysis for transcripts showed some induction of Gm.FT2a in shoot tips and axillary buds in response to rICD conditions (data not shown).

Example 2 Characterization of expression profiles of the pAt.Erecta promoter in soy.

[148] To achieve the desired traits or phenotypes through transgenic approaches, regulation of the temporal and spatial profiles of ectopic FT gene expression may be required. Physiological experiments with soybean to determine the expression of Gm.FT2a in vegetative tissues after short-day induction conditions (see Fig. 2) showed that the achievement of positive yield traits may be based on earlier expression of FT during the vegetative stage. On the other hand, despite the fact that FT transcripts are not detected in the vegetative apices, the FT protein has been shown to travel a long distance from the leaves to the apical tissue, where it initiates the transition from the vegetative to the generative stage. See, for example , Lifschitz, E. et al., (2006), supra ; and Corbeiser, L. et al., ʺFT Protein Movement Contributes to Long-Distance Signaling in Floral Induction of Arabidopsisʺ , Science 316 : 1030-1033 (2007). Thus, in light of our own observations, we proposed using a vegetative stage promoter that is active in the meristem to regulate ectopic FT expression in the plant. By expressing the morphogenetic FT signal directly in the meristem at the required developmental stage, many endogenous pathways and regulatory responses can be bypassed or avoided ( eg , regulation of FT transduction in leaves and long-range FT signal translocation). Previous experiments with short day induction conditions (described in Example 1 above) revealed upregulation of the Gm. Erecta gene in soybean plant meristems. The pErecta promoter (SEQ ID NO: 31) from Arabidopsis has been shown to be weakly expressed in the plant meristem during the vegetative stages of development. Accordingly, the pAt. Erecta promoter was chosen for the first FT expression experiments.

[149] Additional experiments were performed to further explore the expression profiles of pAt. Erecta fused to the GUS reporter gene in vegetative and floral meristematic tissues. Analysis of GUS expression profiles during soybean seedling development showed that the pAt.Erecta promoter exhibits a temporal and spatial expression profile, preferably in meristematic tissues during the vegetative stage of development. Fig. 3A-3O and 4A-4O and FIG. 5A-5F and 6A-6F include two sets of images to illustrate the GUS staining profile. On FIG. 3A-3O and 5A-5F are black and white images of dyed fabrics, and FIGS. 4A-4O and 6A-6F, respectively, are black and white images corresponding to FIGS. 3A-3O and 5A-5F, respectively, but with color filtering to show the profile and intensity of GUS blue staining. Thus, the GUS staining expression profile can be assessed from these black and white images by comparing the corresponding images in FIG. 3A-3O and 4A-4O or FIG. 5A-5F and 6A-6F. As illustrated in FIG. 3A-3O and 4A-4O, GUS staining was detected in immature soybean unifolia and petioles (FIGS. 4A and 4B) three days after seeding/germination. The expression of pAt.Erecta::GUS was also well detected in areas of trifoliate primordia, shoot apical meristem (SAM) and axillary meristem at this early vegetative stage (Fig. 4C). GUS activity was not detected in fully expanded single and trifoliate leaves ten days after germination or seeding (FIGS. 4D and 4E). However, GUS activity was detected in the proximal part of the immature, unfolded, but fully developed trifoliate plate and on the adaxial side of the petiole (Fig. 4F). Detailed observation of the developing apical tissue showed that strong expression was maintained in the developing immature leaf primordia, axillary meristems, and shoot apical meristems (Fig. 4G-I).

[150] At the early generative stage, pAt.Erecta promoter activity was not detected in mature leaf blades and was reduced in the developing leaf primordia. The GUS signal was not detected at the intermediate vegetative stage in the tips in the shoot apical meristem (SAM) or in the axillary meristem (AM) after inflorescences began to form in these tissues (Fig. 4J-4L). At all later stages no additional meristematic activity could be detected in the apices or axils of the flower primordia. However, GUS expression continued on the adaxial side of the petioles and in the proximal part of the immature leaf blade (Fig. 4M and 4N), but not in the fully unfolded leaf blade (Fig. 4O). GUS expression profiles with the pAt.Erecta promoter were also analyzed at later stages of R1 development (35-40 days after germination). Similar to earlier stages of development, expression was not detected in mature leaves or stems. However, strong promoter activity was detected in inflorescence stems (FIGS. 5A and 6A; see arrow) and pedicels (FIGS. 5B and 6B; see arrow). In both tissues, expression was detected in vascular and parenchymal cells (FIGS. 5C and 6C). At this stage, expression was also detected in filaments (FIGS. 5D and 6D; see arrows) and in unpollinated ovules (FIGS. 5E, 5F, 6E and 6F; see arrows in 6F).

[151] Previously, the pAt.Erecta promoter has been studied in Arabidopsis . Interestingly, the expression profiles of pAt.Erecta in Arabidopsis were comparable to those observed in soybean during the vegetative stage, but not during the late generative stages. In contrast, the expression profile of pAt.Erecta in soybean is reduced in early generative tissues, but increases again in some later generative organs and tissues, including inflorescence stems and pedicels. See, for example , Chen, MK et al., FEBS Letters 588 : 3912-17 (2014); Yokoyama, R et al.; Shpak, ED et al., Science 309 : 290-293 (2005); and Yokoyama, R et al., Plant J 15 (3): 301-310 (1998), the entire contents of which are incorporated herein by reference. Thus, the pAt.Erecta promoter provides a new expression profile in soy.

Example 3 Expression of the Flowering Locus T gene, Gm.FT2a under the control of pAt. The Erecta promoter changes flowering time and number of pods per node in soybean.

[152] Transgenic soybean plants were obtained by transforming soybean explants with a recombinant DNA molecule ( i.e., a T-DNA-based transformation vector) containing the pAt.Erecta promoter operably linked to the Gm.FT2a gene through Agrobacterium -mediated transformation to create four pAt.Erecta::Gm.FT2a events, which were left for further study. The effect of FT2a overexpression was immediately apparent in R ₀ plants, which were characterized by very early flowering and termination with reduced seed yield ( eg only about 8 seeds/plant). These Gm.FT2a transgenic plants were also short in height and had very few, if any, branches. Segregating R ₁ plants and their progeny were subsequently grown in a greenhouse under long day conditions for initial study and characterization. By growing these plants under long day conditions, the pronounced dwarf phenotypes observed for Gm.FT2a -R ₀ transgenic plants were improved. In these experiments, both homozygous and hemizygous plants grown in a greenhouse under 16-hour daylight hours ( i.e., 16/8-hour day/night photoperiods) flowered much earlier than wild-type null segregants. The Gm.FT2a transgenic plants flowered about 19-22 days after planting or seeding. (see, for example , Figs. 9A-9C). Under these growth conditions, transgenic soybean plants expressing Gm.FT2a additionally had an increased number of beans per node on the main stem compared to the wild-type control (see, for example , Figures 10 and 11, discussed further below).

[153] Plants containing one of the pAt.Erecta::Gm.FT2a transgenic events (event 1) grown under controlled environmental conditions were further analyzed by scanning electron microscopy (SEM). Analysis of the shoot apical meristem (SAM) of these transgenic plants (harvested 7 days after planting) revealed an early transition of AMP into inflorescence meristem (MS) and floral meristem (FM) (Fig. 7). In contrast, wild-type soybean AMP did not differentiate into MS at this growth stage. Similarly, visualization of the axillary meristem of FT2a transformants (harvested 9 days after planting) showed the development of dormant inflorescence meristems (dMS) (or lateral primordial racemes) in MC and FM (Fig. 8), leading to earlier developing flower branches (races) in nodes of these transgenic plants. Examination of additional phenotypic characteristics revealed early flowering at stage V1 in Gm.FT2a expressing soybean plants, which occurred long before the transition to flowering in null segregating plants (FIGS. 9A-9C). These data, combined with the pAt.Erecta ::GUS expression profile described above, indicate that early flowering, and in particular inflorescence and floral meristem formation, was induced by ectopic expression of Gm.FT2a during the vegetative stage in leaf primordia and shoot apical and axillary meristems. seedling meristem. It is believed that the formation of more inflorescence meristems and floral meristems additionally causes earlier appearance and growth of secondary and tertiary racemes, resulting in more productive flowers and pods formed at the node.

[154] Gm.FT2a -transgenic soybean plants not only showed earlier flowering and produced more beans per node on the main stem (compared to segregating null plants), but the effects of Gm.FT2a ectopic expression in transgenic plants also appeared to be dose-dependent. . Although both homozygous and hemizygous plants had shorter height and less branching, plants homozygous for the Gm.FT2a transgene were more affected than hemizygous plants, possibly because homozygous plants contain two copies of the transgene ( i.e., more dose) as opposed to only one copy ( i.e., lower dose) in hemizygous plants. Under long day growth conditions, homozygous plants died earlier and had a lower overall height with fewer nodes and branches on the main stem compared to plants hemizygous for the transgene (Fig. 10). Unlike homozygous plants, which exhibited a large number of suboptimal dwarf phenotypes, including very few (if any) number of branches on the main stem, hemizygous plants had an intermediate phenotype in terms of vegetative growth, plant height, and the number of nodes present on the main stem, compared with wild-type and homozygous plants. Under 16-hour long day conditions, hemizygous plants had more normal plant height with some degree of branching and longer flowering time compared to homozygous plants (FIG. 10). Hemizygous plants also flowered within 40-64 days of R1 initiation, while homozygous plants only flowered for 16-24 days due to early termination.

[155] Additional experiments were performed with plants transformed with the Gm.FT2a construct (3 events) under long day conditions (16 hours) and controlled environmental conditions to further explore dose-response characteristics for hemizygous and homozygous plants. The difference in the number of nodes and pods on the main stem and branches, as well as the average number of pods per node and average height per plant are shown in Table 1 for three homozygous events (homo event 2, homo event 3, homo event 4) and three hemizygous events (hemi-event 2, hemi-event 3, hemi-event 4). These events are different from Event 1 above.

Table 1. Event rate data for homozygous and hemizygous Gm.FT2a transgenic plants.

Zygosity-Event # Avg. # HS nodes per plant Avg. # VT nodes
per plant Avg. # HS beans
per plant Avg. # of beans W
per plant Avg. Beans per knot Avg. Height (inches) per plant Homo event 2 11.8 6.9 46 9 2.9 17.5 Homo event 3 12.3 6.5 66.4 9 four 21 Homo event 4 12.5 6.8 49.6 9.1 3 19.5 Hemi Event 2 25.3 12.4 183.5 47.3 6.1 37.5 Hemi Event 3 23.9 13.2 200.3 28.8 6.1 40 Hemi Event 4 25.4 15.3 186.8 58 6 41.5

[156] As shown in Table 2, hemizygous plants had a correspondingly higher number of nodes on the main stem (HS) and branches (BT) and greater plant height than homozygous plants. Thus, hemizygous plants are generally less affected than homozygous plants and more closely resemble wild-type plants. Hemizygous plants also had more pods per node and more pods per main stem and branches compared to homozygous plants. Therefore, hemizygous plants in general had a more normal plant architecture with a higher number of pods per node (and per plant), presumably due to a lower dose of the Gm.FT2a transgene. The relative dose level of Gm.FT2a based on transgene zygosity was further confirmed by additional experiments showing that Gm.FT2a transcript levels were higher in tissues from homozygous plants than in tissues from hemizygous plants (data not shown).

[157] Early flowering induction in these Gm.FT2a transgenic plants was associated with a higher number of pods (and seeds) per main stem node in both hemizygous and homozygous plants. Homozygous and hemizygous plants containing the Gm.FT2a transgene had a higher number of pods/seeds per node on the main stem of the plant compared to wild-type segregants (Fig. 11). It was also found that the distribution of beans on the main stem is different for Gm.FT2a transgenic plants and wild-type null plants. Both homozygous and hemizygous plants grown under long day conditions were found to have more pods at least at the lower nodes of the main stem and more pods per node on average compared to wild-type null plants (data not shown). Plants hemizygous for the Gm.FT2a transgene contained the highest number of pods per node along the entire length of the main stem. However, these effects depended on specific growth conditions, including day length, etc. In general, experiments with soybean under longer day conditions showed a trend towards greater difference between transgenic and null plants.

[158] Dose-dependent effects of transgene expression of Gm.FT2a were also observed in field experiments. In field experiments, soybean plants hemizygous for the two Gm.FT2a events (events 1 and 2, above) averaged about 2.68 pods per node on the main stem, and plants homozygous for these events averaged about 1.40 beans per node, while null segregating plants had about 1.63 beans per node. However, in early field trials, plants hemizygous for the transgenic Gm.FT2a (event 2) were found to have an average of about 3.21 pods per node compared to about 3.05 pods per node on average in homozygous plants and about 2.19 beans per node in null segregating plants. In another small-plot experiment conducted at a different field location, plants hemizygous for the Gm.FT2a transgene (event 1) were found to have an average of about 2.17 pods per node compared to an average of about 2.05 pods per node. in plants homozygous for the Gm.FT2a transgene (event 2) and about 1.30 beans per node in null segregating plants. Thus, the number of beans per node in plants containing the Gm.FT2a transgene may depend on a variety of factors, including the dose of the FT transgene, environmental and field conditions, and possibly differences in agricultural practices. However, like Gm.FT2a transgenic plants grown in a greenhouse, homozygous and hemizygous Gm.FT2a transgenic plants grown in the field often had fewer main stem nodes, lower overall height and/or reduced branching in transgenic plants. In fact, wild-type plants tend to have more branching and a higher total number of nodes per plant than hemizygous and homozygous Gm.FT2a plants .

[159] Additional physiological data were obtained from homozygous Gm.FT2a transgenic plants and control wild-type (WT) plants grown in a greenhouse under 14-hour long day conditions (see Table 2). These data represent average measurements made of six Gm.FT2a transgenic plants for each event or eight wild type plants.

[160] To obtain the phenotypic characteristics of these plants, the following matrices were collected: Days to Flowering on R1 (DOFR1); days to R7 (DOR7); duration of the generative stage on days R1 to R7 (PDR1R7); number of branches per plant (BRPP); total number of fertile nodes on branches (FNBR); total number of fertile nodes per plant (FNLP); total number of fertile nodes on the main stem (FNST); number of nodes on branches (NDBR); number of nodes on the main stem (NDMS); number of nodes/plant (NDPL); percentage of fertile nodes on branches (PFNB); percentage of total number of fertile nodes (PFNN); percentage of fertile nodes on the main stem (PFNS); number of pods per plant (PDPP); number of beans per main stem (PODMS); number of beans per branch (PODBR); average number of beans/node; number of seeds per plant per R8 (SDPPR8); and weight of 1000 seeds (SW1000). Each of these measurements was taken at the time of collection, unless otherwise indicated.

Table 2. Construct level phenotypic data for transgenic Gm.FT2a homozygous plants and DT plants.

DT pAt.Erecta::Gm.FT2a DOFR1 33.5 21.3 DOR7 106.9 92.9 PDR1R7 76.5 71.6 BRPP 20.1 one FNBR 190.6 2 FNLP 214.6 fifteen FNST 24.0 14.3 NDBR 211.4 3 NDMS 33.4 15.3 NDPL 244.9 16.3 PDPP 575.8 61.2 PFNB 90.4 75 PFNN 87.8 92.0 PFNS 71.4 92.9 PODBR 487.3 3 PODMS 88.4 60.2 Bobs/Knot 2.4 3.8 SDPPR8 1319.6 122.1 SW1000 (grams) 146 122.5

[161] Consistent with the above observations, homozygous Gm.FT2a transgenic plants experienced earlier flowering induction than DT plants (DOFR1 about 21 days after planting instead of about 33-34 days for wild-type plants). These measurements further showed that the number of branches (and other branching-related measurements such as the number of nodes or beans on branches) was greatly reduced. Since the transgenic plants were shorter and had very little branching, the total number of nodes or pods per plant was also greatly reduced. However, the number of pods per node on the main stem was increased in transgenic plants ( eg averaging about 3.8 pods/node) compared to wild-type null plants ( eg approx 2.4 pods/node).

[162] Without wishing to be bound by theory, it is believed that the higher pods per node observed for transgenic soybean plants expressing FT2a in the meristem during the vegetative stages of development may be at least in part due to the synchronization of early flowering with early emergence of secondary and /or tertiary racemes and/or better use of resources with more bean-bearing flowers per node. Early expression of FT in the meristem (see e.g. Figs. 3 and 4) can cause early emergence of dormant inflorescence meristems producing more racemes per plant node so that more racemes produce mature flowers and fully developed pods at each node. However, subsequent expression of FT in the generative tissue (see, for example , Fig. 5 or 6) can abort the floral development of later developing flowers at each node, leading to more efficient resource allocation in earlier developing racemes, flowers, and pods. In wild-type soybean plants, a much lower percentage of secondary and tertiary racemes produced flowers and fully developed pods compared to primary racemes, and later developing primary raceme flowers generally do not produce mature flowers and/or full-sized pods before dropping. Thus, it has been suggested that a higher number of pods per node may be formed in plants expressing FT proteins in the vegetative meristem by synchronizing early flower development with the early appearance of lateral racemes at one or more plant nodes. With at least the pAt . Erecta promoter driving FT expression, later developing flowers (which might otherwise not produce fully developed or full-sized pods) can also be terminated by FT expression at a later generative stage to direct resources to earlier developing flowers.

Example 4 Expression of the Flowering Locus T Gene, Gm.FT2a , Driven by Alternative Vegetative Stage Promoters in Soybean.

[163] Based on the phenotypes observed in the previous example 3, two promoters were proposed to drive the expression of the Gm.FT2a transgene, which are considered leaf-preferred vegetative stage promoters: pAt.BLS (SEQ ID NO: 46) and pAt.ALMT6 ( SEQ ID NO: 47). As used herein, "leaf-preferred" refers to a promoter that preferentially initiates transcription of its associated gene in leaf tissues over other plant tissues. Since FT is thought to function as a mobile florigen, early expression of FT during the vegetative stages in peripheral tissues such as leaves with a leaf-preferred or leaf-specific promoter can result in phenotypes similar to meristem-preferred pAt expression. Erecta::Gm.FT2a . Additionally, it has been suggested that expression of FT with a vegetative leaf promoter may also attenuate the flowering induction signal and therefore mitigate the early termination phenotypes seen with homozygous expression of FT in the meristem and increase plant height and branching.

[164] In these experiments, transformation vectors for pAt.ALMT6 :: Gm.FT2a and pAt.BLS :: Gm.FT2a were constructed and used to transform a soybean line via Agrobacterium -mediated transformation. It was shown that expression with the pAt.BLS promoter starts in leaf primordia number 5 (p5) and is expressed in primary leaf veins only before the transition to flowering, while the pAt.ALMT6 promoter is also a vegetative leaf promoter with expression at later stages of development compared to with pAt.BLS . See, for example , Efroni et al., "A Protracted and Dynamic Maturation Schedule Underlies Arabidopsis Leaf Development," The Plant Cell 20 (9): 2293-2306 (2008); and Shani et al., "Stage-Specific Regulation of Solanum lycopersicum Leaf Maturation by Class 1 KNOTTED1-LIKE HOMEOBOX Proteins," The Plant Cell 21 (10): 3078-3092 (2009). Transgenic soybean plants were generated for each of these vector constructs and studied for phenotypes in growth chambers under 14 hour photoperiod conditions compared to wild type plants. Six transgenic events (5 plants per event) were tested for each of the pAt.BLS promoter constructs, and seven transgenic events (5 plants per event) were tested for the pAt.ALMT6 promoter. For each of these constructs, controls were obtained from five wild-type plants.

[165] To obtain the phenotypic characteristics of these transgenic plants, the following matrices were assembled (Tables 3 and 4). Individual measurements are similar to those defined above, and phenotypic characterization was performed on plants homozygous for the transgene.

Table 3. Construct level phenotypic data for pAt.ALMT6::Gm.FT2a plants and DT plants.

DT pAt.ALMT6::Gm.FT2a DOFR1 35.2 38.8 DOR7 84.7 88.8 PDR1R7 49.5 50.0 BRPP 7.7 8.9 FNBR 57.8 73.3 FNLP 69.7 85.0 FNST 12.0 11.7 NDBR 78.9 96.0 NDMS 21.3 22.5 NDPL 100.2 118.5 PDPP 120.2 141.1 PFNB 73.2 76.8 PFNN 71.7 72.1 PFNS 57.9 51.7 PODBR 91.8 118.1 PODMS 28.3 22.9 Bobs/Knot 1.4 1.2

Table 4. Construct level phenotypic data for pAt.BLS::Gm.FT2a plants and DT plants.

DT pAt.BLS::Gm.FT2a DOFR1 31.3 35.2 DOR7 78.1 82.6 PDR1R7 46.9 47.5 BRPP 7.5 8.8 FNBR 65.7 81.2 FNLP 80.5 94.0 FNST 14.9 12.7 NDBR 72.2 95.6 NDMS 21.9 22.3 NDPL 94.0 117.9 PDPP 137.0 148.1 PFNB 92.3 85.3 PFNN 87.4 80.1 PFNS 68.1 57.3 PODBR 100.9 123.4 PODMS 36.1 24.8 Bobs/Knot 1.7 1.3

[166] Transgenic plants expressing Gm.FT2a under the control of alternative promoters pAt.ALMT6 and pAt.BLS were phenotypically more similar to wild-type (WT) plants than transgenic pAT.Erecta :: Gm.FT2a plants . Plants transformed with constructs pAt.ALMT6::Gm.FT2a and pAt.BLS::Gm.FT2a had flowering times and vegetative growth characteristics similar to wild-type controls, possibly with a slightly increased number of nodes per branch compared to plants wild type (tables 3 and 4). These data can be interpreted as showing that the timing and location of transgenic FT expression is important in obtaining generative and yield-related traits or phenotypes that differ from wild-type plants. Mere expression of the FT transgene during earlier vegetative stages of development ( eg in leaf tissues) may not be sufficient to change generative and yield-related plant phenotypes ( eg number of pods per node). Thus, according to embodiments of the present invention, a promoter operably linked to a florigenic FT transgene may preferably be a meristem-specific or meristem-preferred promoter in addition to directing expression during the vegetative stages of plant development. However, when expression profiles for the above two leaf-preferred promoters were examined in soybean plants, GUS staining with the pAt.BLS promoter was not observed in developing leaves, and the pAt.ALMT6 promoter did not provide detectable GUS expression in leaves until the late vegetative stages with much more high expression during the early generative stages. Thus, the possibility remains that expression of FT transgenes in peripheral tissues (leaves) during early vegetative stages using different tissue-specific promoters may be sufficient, in some cases, to induce early flowering and/or other generative and yield-related traits or phenotypes, which may also depend on the particular plant species being studied.

Example 5 Identification of protein domains of FT homologues using Pfam analysis.

[167] Gm.FT2a orthologs were identified by sequence analysis and literature review, and several examples of these FT homologues are listed in Table 5 along with Gm.FT2a . These include other soybean FT genes as well as several FT genomes from other plant species. The amino acid sequences of these FT proteins were analyzed to identify any Pfam protein domains using the HMMER software and the Pfam database (version 27.0). These FT protein sequences (SEQ ID NOs: 2, 4, 6, 8, 10 and 12) were found to have the same Pfam domain defined as a phosphatidylethanolamine binding domain (PEBP) protein having the Pfam domain name "PBP_N" and Pfam access number PF01161. The location of the PBP_N domains in each of these FT protein sequences is also shown in Table 5. The location of the PBP_N domain in other FT proteins can be determined by sequence alignment. Therefore, it is contemplated that any DNA sequence encoding at least a FT protein containing a PBP_N domain can be used in the recombinant DNA molecule of the present invention, provided that the corresponding FT protein has florigenic activity when ectopically expressed in the plant meristem.

Table 5. Location of the PBP_N (Pfam) domain in FT protein sequences.

PROTEIN
SEQID NO. Gene name Domain location 2 Gm.FT2a 28-162 four Gm.FT5a 26-157 6 Gm.FT2b 28-162 eight Zm.ZCN8 26-154 ten Nt.FT-like 25-159 12 Le.SFT 29-161

Example 6 Promoter driven expression of FT homologuespAt.Erecta in soy.

[168] Additional transformation vectors were constructed containing other FT homologues (Table 6) under the control of the pAt . Erecta promoter and used to transform soybean via Agrobacterium -mediated transformation. The transgenic plants generated by these events were studied for their phenotypes in a greenhouse with 14-14.5 hour natural photoperiods. Six events were examined for each construct (6 plants per event). Six plants were also examined and averaged over wild-type control (WT) plants. Different sets of experiments (AE) were performed as shown in Table 6 with separate wild-type controls.

Table 6 List of designs for someGm.FT2a and their homologues with their protein sequences.

Design Description Gene name PROTEIN SEQ ID NO. Study group pAt.Erecta::Gm.FT2a Gm.FT2a 2 A pAt.Erecta::Gm.FT2b Gm.FT2b 6 C pAt.Erecta::Gm.FT5a Gm.FT5a four E pAt.Erecta::Zm.ZCN8 Zm.ZCN8 eight B pAt.Erecta::Nt.FT-like Nt.FT-like ten B pAt.Erecta::Le.SFT Le.SFT 12 D

[169] The following matrices were assembled to obtain the phenotypic characteristics of plants transformed with each of the constructs listed in Table 6 to express FT homologues with the pAt.Erecta promoter in addition to the data generated for the Gm.FT2a construct as described above. Separate measurements are similar to those defined above, and the production of phenotypic character transformants was performed for plants homozygous for the transgene.

[170] Phenotypic data were obtained for plants expressing the Zm.ZCN8 and Nt.FT-like transgenes under the control of the pAt.Erecta promoter (see tables 7 and 8). The characteristic values for each event in tables 7 and 8 are averaged over all the studied plants containing the event. There is also a column with averaging event values for each feature.

Table 7. Phenotypic construct level data and events for Zm.ZCN8 plants and DT plants.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 33.5 28.6 29 29.2 27.5 27 30.7 28 DOR7 106.9 93.5 97.3 89.2 88.2 93.5 100.3 92.8 PDR1R7 76.5 64.1 69.8 60 59 59.5 71.7 64.8 BRPP 20.1 3.2 2.8 1.3 1.5 1.3 9.5 3 FNBR 190.6 26.9 32 eight 7 2.3 95.3 17 FNLP 214.6 54.9 67.5 28 40.5 20.3 132.5 40.5 FNST 24.0 28.3 35.5 22 33.5 eighteen 37.3 23.5 NDBR 211.4 30.2 32.5 9 7.5 3.5 110.8 17.8 NDMS 33.4 30.5 36.3 24 34.3 twenty 44.8 24 NDPL 244.9 60.3 68.8 30.8 41.8 23.5 155.5 41.8 PDPP 575.8 317.5 498.3 144.8 319 76.3 658 208.8 PFNB 90.4 87.2 98.6 90.3 85.4 64.6 91.1 93.2 PFNN 87.8 93.1 98.1 92.3 97.0 86.5 88.5 96.4 PFNS 71.4 93.2 97.9 92.5 97.9 90.6 82.1 98.1 PODBR 487.3 105.4 162 19 18.5 3.3 384.5 45.3 PODMS 88.4 212.9 336.3 130.5 300.5 73 273.5 163.5 Bobs/Knot 2.4 5.5 7.2 4.6 7.7 3.2 4.9 5.2 SDPPR8 1319.6 564.7 961 200.5 562 136.8 1166.3 361.5 SW1000
(grams) 146 108.9 102.9 127.4 105.3 82.9 116.6 117.9

Table 8 Phenotypic construct level data and events for plantsNt.FT-like and plants DT.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 33.5 31.5 39.3 27.7 25.3 29 37.2 30.7 DOR7 106.9 93.9 115.8 90.7 80.7 83.7 102.2 90.2 PDR1R7 76.5 62.3 76.4 63 55.3 54.7 65 59.5 BRPP 20.1 9.8 twenty 8.3 2.3 5.3 17 6 FNBR 190.6 108.7 190.5 95.3 eleven 54.3 223 78.3 FNLP 214.6 131.4 212.3 118 29.5 77.8 248 103 FNST 24.0 23.2 21.8 22.8 21.3 23.5 25 24.8 NDBR 211.4 128.7 281.8 97 eleven 54.5 247.7 80.5 NDMS 33.4 28.9 33.8 27 23.3 24.8 35.7 28.8 NDPL 244.9 157.1 315.5 124 31.5 79.3 283.3 109.3 PDPP 575.8 462.1 638 511.3 150.8 296 745 431.5 PFNB 90.4 92.5 68.0 98.6 100 99.7 91.3 97.2 PFNN 87.8 89.6 67.6 95.4 93.3 98.3 88.4 94.2 PFNS 71.4 81.9 64.7 83.3 91.3 95.2 70.6 86.2 PODBR 487.3 326.3 529 342.3 22.7 147 633.3 283.5 PODMS 88.4 136.7 109 169 133.8 149 111.7 148 Bobs/Knot 2.4 3.6 2.0 4.3 4.9 3.8 2.7 4.0 SDPPR8 1319.6 928.7 1359.8 965.5 382.7 591.5 1714.3 558.7 SW1000 (grams) 146 149.0 143.7 121.2 133.8 179.3 142.2 174.0

[171] Transgenic soybean plants expressing the Zm.ZCN8 and Nt.FT-like proteins bloomed earlier than wild-type control plants and had an increased number of beans per node (similar to plants expressing the Gm.FT2a transgene). In fact, soybean plants expressing Zm.ZCN8 and Nt.FT-like had several phenotypes similar to Gm.FT2a- transgenic plants, including fewer days to flowering (DOFR1), fewer branches (BRPP), fewer nodes per plant (NDPL), fewer nodes per branch (NDBR), fewer pods per plant (PDPP) and fewer pods per branch (PODBR), along with an increase in the number of pods per node and a decrease in the number of seeds per plant (Tables 7 and 8) compared to wild-type controls. However, several negative phenotypes observed in Gm.FT2a homozygous plants were less pronounced in Zm.ZCN8 and Nt.FT-like transgenic plants expressing. In general, plants expressing the Zm.ZCN8 transgene had shorter height and less branching, but more pods per node on the main stem. Similarly, plants expressing the Nt.FT-like transgene had lower height, less branching, increased number of pods per node on the main stem compared to wild-type control plants.

[172] Two Zm.ZCN8 transgenic events and four Nt.FT-like events listed above were also field tested at two different locations. Phenotypic data were obtained for plants expressing the Zm.ZCN8 and Nt.FT-like transgenes under the control of the pAt.Erecta promoter (Tables 9 and 10). In the case of field measurements, the traits were similar to those listed above for the greenhouse data tables, except that DOR8 and PDR1R8 represent the number of days before R8 and the duration of the generative stage between R1 and R8. In addition, all other traits were determined at harvest (ie, R8 step) instead of R7 step. Events 1 and 2 in Table 9 correspond to Events 2 and 3 in Table 7, and Events 1-4 in Table 10 correspond to Events 1-4 in Table 8, respectively. With the exception of the number of days to flowering on R1 (DOFR1) and the duration of the generative stage in days from R1 to R8 (PDR1R8), all phenotypic measurements are based on data collected at two locations. Similar to greenhouse observations, transgenic soybean plants expressing Zm.ZCN8 and Nt.FT-like proteins also flowered earlier than wild-type controls in the field. The Zm.ZCN8 transgenic plants had an increased number of pods per node, while the Nt.FT-like plants did not show a clear increase in the number of pods per node in field trials.

Table 9 Phenotypic data from field trials for Zm.ZCN8 plants and DT plants.

DT Average Event 1 Event 2 DOFR1* 42.4 27.9 28.0 27.7 DOR8 110.7 95.0 92.0 98.0 PDR1R8* 65.7 67.1 63.5 70.7 BRPPR8 2.6 0.1 0.2 0.0 NDBRR8 9.7 0.3 0.5 0.1 NDMSR8 18.3 13.6 12.5 14.7 NDPLR8 28.0 13.9 13.0 14.8 PDPPR8 44.2 35.1 30.1 40.0 TPBRR8 9.5 0.3 0.5 0.1 PODMSR8 34.7 34.7 29.5 39.9 Bobs/Knot 1.6 2.5 2.3 2.6 SDPPR8 99.9 67.6 54.7 80.5 SW1000 (oz) 5.1 4.1 3.8 4.3

(* - data for one location)

Table 10 Phenotypic data from field trials for plantsNt.FT-likeand plants DT.

DT Average Event 1 Event 2 Event 3 Event 4 DOFR1* 42.4 38.0 42.5 26.8 26.8 25.8 DOR8 110.7 93.3 111.3 88.2 86.6 87.1 PDR1R8* 65.7 63.1 66.8 62.2 60.3 63.0 BRPPR8 2.6 0.7 2.4 0.1 0.2 0.1 NDBRR8 9.7 2.7 9.2 0.5 0.8 0.3 NDMSR8 18.3 11.5 18.3 9.9 7.6 10.1 NDPLR8 28.0 14.2 27.5 10.4 8.5 10.4 PDPPR8 44.2 23.5 43.0 18.9 11.6 20.3 TPBRR8 9.5 2.6 8.4 0.5 0.8 0.5 PODMSR8 34.7 20.9 34.6 18.5 10.8 19.8 Bobs/Knot 1.6 1.6 1.6 1.8 1.4 1.7 SDPPR8 99.9 49.9 98.6 36.3 25.0 39.7 SW1000 (oz) 5.1 4.5 5.1 4.4 4.6 4.0

(* - data for one location)

[173] Additional phenotypic data were obtained for plants expressing the Gm.FT2b transgene under the control of the pAt.Erecta promoter (Table 11).

Table 11. Phenotypic construct level data and events for Gm.FT2b plants and DT plants.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 43.7 34.6 41.2 34.3 22.7 33.2 37.2 39.3 DOR7 105.9 100.4 100.5 100.3 99.8 100.3 98.7 102.8 PDR1R7 62.2 65.8 59.3 66 77.2 67.2 61.5 63.5 BRPP 13.4 4.7 7 5 1.7 3.3 3.7 7.7 FNBR 103.8 32.4 52 29.7 12 30.7 21.7 48.7 FNLP 125.0 46.6 68.7 41.3 24.3 45 37.3 63 FNST 21.2 14.2 16.7 11.7 12.3 14.3 15.7 14.3 NDBR 108.4 34.2 54 30.3 12.7 33.7 24.7 fifty NDMS 30.2 18.0 18.3 15.3 fifteen 19 19.3 21 NDPL 138.7 52.2 72.3 45.7 27.7 52.7 44 71 PDPP 387.4 143.0 167 140 96 145.7 108.7 200.7 PFNB 95.5 94.6 96.4 97.7 96.8 91.0 87.7 97.7 PFNN 90.1 89.1 94.9 90.3 88.0 86.0 86.1 89.4 PFNS 69.7 79.2 91.5 74.5 82.5 77.0 81.1 68.7 PODBR 284.9 90.2 109.3 96 43 94.7 55.3 143 PODMS 102.5 52.7 57.7 44 53 51 53.3 57.7 Bobs/Knot 2.8 2.7 2.3 3.1 3.5 2.8 2.5 2.8 SDPPR8 1159.3 322.3 411.3 292.3 195.3 346.7 245 443.3 SW1000
(grams) 174.0 154.0 170.4 156.7 154.1 155 130.2 157.8

[174] Transgenic soybean plants expressing the Gm.FT2b transgene flowered earlier and had less branching than wild-type control plants. Gm.FT2b-expressing soybean plants were characterized by reduced number of days to flowering (DOFR1), reduced number of branches (BRPP), fewer nodes per plant (NDPL), fewer nodes per branch (NDBR), reduced number of pods per plant (PDPP) and fewer pods per branch (PODBR) (Table 9). However, the Gm.FT2b transgenic plants did not show an increase in the number of pods per node. In general, plants expressing the Gm.FT2b transgene had shorter height and less branching compared to wild-type control plants. Transgenic soybean plants expressing four different events of the Gm.FT2b transgene were also tested in field experiments. Phenotypic data were obtained for plants expressing the Gm.FT2b transgene under the control of the pAt.Erecta promoter (Table 12). Events 1-4 in Table 11 correspond to Events 3, 2, 1, and 4 in Table 12, respectively. Similar to greenhouse observations, Gm.FT2b -expressing soybean plants showed a reduced number of days to bloom (DOFR1) in the field. Other phenotypic measurements also showed similar traits to those observed in the greenhouse compared to wild-type controls.

Table 12 Phenotypic data from field trials for Gm.FT2b plants and DT plants.

DT Average Event 1 Event 2 Event 3 Event 4 DOFR1 41.9 37.3 38.3 38.3 36.3 36.2 DOR8 115.4 109.2 111.1 110.6 110.6 104.6 PDR1R8 73.5 71.8 75.0 72.1 74.1 66.1 SDPPR8 188.5 95.5 99.4 81.1 117.7 83.6 SW1000 (grams) 153.4 137.3 144.0 129.6 134.4 141.5

[175] Additional phenotypic data were obtained for plants expressing the Le.SFT transgene under the control of the pAt.Erecta promoter (Table 13).

Table 13. Phenotypic construct level data and events for Le.SFT plants and DT plants.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 42.9 41.4 thirty 44.4 30.7 28 60.2 55 DOR7 108.6 103.8 90.7 106.2 99 91.5 116.5 119 PDR1R7 65.5 64.0 60.2 71 67 65 56.4 64.2 BRPP n/a n/a n/a n/a n/a n/a n/a n/a FNBR 131.5 37.1 2.7 125.2 4.7 one 47.7 41.3 FNLP 156.8 50.7 15.7 142.6 18.1 18.3 56.7 52.7 FNST 25.3 13.7 13 17.7 13.7 17.3 9 11.3 NDBR 140.2 38.9 3 129.4 5.4 one 51 43.7 NDMS 32.4 17.9 16 25.8 16.3 21 12.3 16.3 NDPL 172.4 56.7 19 154.7 21.2 22 63.3 60 PDPP 473.3 201.3 53.7 432.3 69.8 85.7 279.3 287 PFNB 94.1 94.0 100 96.9 83.3 100 92.9 90.7 PFNN 90.4 86.4 82.5 92.3 83.7 83.5 88.8 87.6 PFNS 77.1 76.5 81.2 69.5 84.0 82.8 72.7 68.6 PODBR 366.2 141.8 3.7 361.5 15.5 1.3 238.7 230.7 PODMS 114 60.3 fifty 73.6 57.1 84.3 40.7 56.3 Bobov/
knot 2.7 3.6 2.8 2.8 3.3 3.9 4.4 4.8 SDPPR8 1247.4 476.0 136.7 1036 148.5 183 655 697 SW1000
(grams) 167.7 153.2 170.0 182.8 157.5 148.5 131.5 128.8

[176] In general, soybean plants expressing the transgeneLe.SFT, had lower height with less branching and an average increased number of pods per node compared to wild-type controls (Table 13). However, these effects varied and depended on the event. For example, events 1, 3 and 4 showed early flowering (DOFR1), while other events were neutral or actually delayed flowering. In addition, some of the transgenic eventsLe.SFTshowed an increase in the number of beans per node on average to varying degrees, while a couple of events were neutral in terms of the number of beans per node. Interestingly, two events (events 5 and 6) had the highest number of pods per node on average, despite delayed flowering.

[177] Additional phenotypic data were obtained for plants expressing the Gm.FT5a transgene driven by the pAt.Erecta promoter (Table 14).

Table 14. Phenotypic construct level data and events for Gm.FT5a plants and DT plants.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 DOFR1 48.2 29.9 32.2 29 28.6 29.2 30.5 DOR7 110 92.5 96.6 90.4 91 92.8 91.8 PDR1R7 61.8 62.7 64.4 61.4 62.4 63.6 61.3 BRPP 12.4 2.5 7 1.7 one 1.3 1.7 FNBR 105.6 7.3 20.3 4.7 3 four 4.3 FNLP 126.5 24.5 41.7 twenty 18.7 19.3 22.7 FNST 20.9 17.2 21.3 15.3 15.7 15.3 18.3 NDBR 108.6 7.5 21 5 3 four 4.3 NDMS 29 17.7 22 15.7 16.3 16 18.7 NDPL 137.6 25.2 43 20.7 19.3 twenty 23 PDPP 304.3 131.9 214.7 111 100.3 104.3 129.3 PFNB 97.2 98.0 97.3 93.3 100 100 100 PFNN 98.1 97.0 95.9 90 99.1 100 100 PFNS 72.1 97.0 97.1 98.1 96.1 95.8 97.9 PODBR 233.4 16.5 60.5 eight four 6 four PODMS 75.1 108.6 159 98.5 95 92.5 98 Bobs/Knot 2.2 5.2 5.0 5.4 5.2 5.2 5.6 SDPPR8 778.8 271.7 516 232.7 175.3 182.3 252 SW1000
(grams) 151.6 126.0 143.7 122.4 122.2 121.8 116.8

[178] Transgenic soybean plants expressing the transgeneGm.FT5a, flowered significantly earlier than wild type controls and had an increased number of pods per node (similar to plants expressing the transgeneGm.FT2a). In fact, soybean plants expressingGm.FT5a, had several phenotypes (similar toGm.FT2a-transgenic plants), including fewer days to flowering (DOFR1), fewer branches (BRPP), fewer nodes per plant (NDPL), fewer nodes per branch (NDBR), fewer pods per plant (PDPP) and fewer pods per branch (PODBR), along with an increase in the number of pods per node and a decrease in the number of seeds per plant (Table 14). In general, plants expressing the transgeneGm.FT5a, had lower height and less branching, but more pods per node (particularly on the main stem) compared to wild-type controls.

[179] While not wishing to be bound by theory, these data support a model of the effect of FT overexpression in a dose-dependent manner with the degree or measure of associated phenotypes ( e.g. , early flowering, increase in pods per node, and change in plant architecture) as a function of (i) level and time FT expression, (ii) tissue specificity of FT expression, and (iii) relative activity and target specificity of the particular FT protein expressed. For example, expression of FT protein orthologues from other plant species in soybean may indicate a weaker effect compared to overexpression of endogenous FT protein (Gm.FT2a) in soybean, which may be due to non-native FT protein homologues having lower activity in soybean. . However, the expression of some native FT proteins may not result in significant phenotypic effects if they have a different specialized role in their native state or context. Different FT proteins can also act on different tissue targets and receptors and therefore have different effects on different plant architecture and flowering traits and phenotypes.

[180] Regardless of the level of activity of a particular FT homologue, changes in plant generativity and architecture-related phenotypes correlate with the timing and location of FT expression. Expression of FT transgenes during the vegetative stage may be necessary to induce early flowering and/or increase the number of floral meristems, flowers, pods, etc. per plant node. In fact, expression of FT in meristematic tissues during vegetative stages with an appropriate dose of the FT transgene has been shown to induce generative changes in plants that result in an increase in the number of flowers, pods and/or seeds per node. In contrast, expression of the Gm.FT2a transgene driven by leaf-preferred promoters resulted in very small, if present, phenotypic changes compared to wild-type plants. These data indicate that both timing and tissue specificity (or tissue preference) of FT expression are important factors that influence generative and/or yield-related phenotypic changes in transgenic plants.

[181] The data presented suggest that different FT proteins may have different levels of activity and/or different tissue specificity despite being expressed with the same pErecta promoter. Although several constructs expressing Gm.FT2a , Zm.ZCN8 , Nt.FT- like, and Gm.FT5a caused early flowering and termination in addition to increasing the number of pods per node, other constructs expressing Gm.FT2b and Le.SFT had other correlative effects. flowering effects. Expression of Gm.FT2b did not cause early flowering and termination of plants, but did not show a significant increase in the number of pods per node. On the other hand, Le.SFT expression showed an increase in the number of pods per node and early termination despite delayed flowering. Interestingly, the increase in the number of pods per node in FT transgenic plants does not correlate with prolongation of the generative stage (PDR1R7) and does not always accompany early flowering (DOFR1), as noted above. These data suggest that generative changes in response to expression of FT proteins in the meristem during the vegetative stage may occur through one or more independent mechanisms or pathways. The increase in the number of pods per node in FT transgenic plants may depend on the number of inflorescence meristems and floral meristems induced from vegetative meristems at each node, which may occur regardless of flowering time and/or duration of the generative stage. However, as noted above, the duration of the generative stage may not necessarily correlate with the duration of flowering.

Example 7 Identification of Additional Vegetative Stage Meristematic Promoters.

[182] After observing the phenotypic effects with Gm.FT2a expression under the control of the meristem-preferred vegetative stage promoter pAt.Erecta , it is contemplated that other meristem-preferred (meristem-specific) vegetative stage promoters can be used to drive the expression of FT proteins to initiate associated the generativity or yield of traits or phenotypes in plants, such as increased number of pods per node (and/or per plant or main stem). Using the studied expression profile of the pAt.Erecta promoter (see Example 2), other meristem-preferred (meristem-specific) vegetative stage promoters from soybean, potato and Arabidopsis were identified. Two bioinformatic approaches were used to identify candidate genes from other dicotyledonous species, including, for example, Arabidopsis, soybean, Medicago , potato, and tomato, having similar expression profiles to pAt . Erecta: BAR Espressolog and Expression Angler. See, for example , BAR expressolog identification: expression profile similarity ranking of homologous genes in plant species,ʺ Plant J 71 (6): 1038-50 (2012); and Toufighi, K et al., "The Botany Array Resource: e-Northerns, Expression Angling, and promoter analyses," Plant J 43 (1): 153-163 (2005). Therefore, promoter sequences from these genes have been proposed for use in the expression of FT transgenes in accordance with embodiments of the present invention.

[183] Examples of gene promoters identified in this assay include the following: four soybean receptor-like kinase (RLK) genes including Glyma10g38730 (SEQ ID NO: 33), Glyma09g27950 (SEQ ID NO: 34), Glyma06g05900 (SEQ ID NO: 35) and Glyma17g34380 (SEQ ID NO: 36). Additional examples include receptor-like kinase (RLK) gene promoters from potato, PGSC0003DMP400032802 (SEQ ID NO: 37) and PGSC0003DMP400054040 (SEQ ID NO: 38). It is possible that these RLK genes may be structurally and/or functionally related to the Erecta and Erecta-like genes from Arabidopsis and other species, as they are also RLK genes. Other meristem-preferred vegetative stage promoters from Arabidopsis genes include the following: At.MYB17 ( At.LMI2 ; At3g61250) (SEQ ID NO: 41), Kinesin-like gene (At5g55520) (SEQ ID NO: 42), AP2/B3- like genes including At.REM17 (SEQ ID NO: 43) or At.REM19 and erecta -like 1 and 2 genes, At.Erl1 (SEQ ID NO: 44) and At.Erl2 (SEQ ID NO: 45). Each of these promoters and similar functional sequences can be operably linked to the FT gene to initiate ectopic expression of FT genes in one or more plant meristems at least during the vegetative stage(s) of development.

[184] For the At.MYB17 ( At.LMI2 ) gene, see Pastore, JL et al., ʺLATE MERISTEM IDENTITY 2 acts together with LEAFY to activate APETALA1 ,ʺ Development 138 : 3189-3198 (2011), the full contents of which are included in this document by reference. For the Kinesin-like gene, see Fleury, D et al., ʺThe Arabidopsis thaliana Homolog of Yeast BRE1 Has a Function in Cell Cycle Regulation during Early Leaf and Root Growth,ʺ Plant Cell , 19 (2): 417-432 (2007) , the entire content of which is incorporated herein by reference. For Arabidopsis REM17 and REM19 genes, see Mantegazza, O et al., ʺAnalysis of the Arabidopsis REM gene family predicts functions during flower development,ʺ Ann Bot 114 (7): 1507-1515 (2014), the full contents of which are incorporated herein through a link. For more on the At.Erl2 gene, see ʺSpecial Issue: Receptor-like Kinases,ʺ JIPB 55 (12): 1181-1286 (2013), and in particular Shpak, E., ʺDiverse Roles of ERECTA Family Genes in Plant Development, ʺ JIPB 55 (12): 1251-1263 (2013), the entire contents of which are incorporated herein by reference.

Example 8 Expression of the Flowering Locus T gene, Gm.FT2a , under the control of the pAt.Erl1 promoter changes the flowering time and the number of pods per node in soybeans.

[185] A transformation vector containing Gm.FT2a under the control of the meristem-preferred vegetative stage promoter pAt.Erl1 (SEQ ID NO: 44) was constructed and used to transform soybean by Agrobacterium -mediated transformation. The transgenic plants generated by these events were studied for their phenotypes in a greenhouse with 14-14.5 hour natural photoperiods. For each pAt.Erl1::Gm.FT2a construct , six events (6 plants per event) were examined in the greenhouse. Six plants were also examined and averaged over wild-type control (WT) plants. Four events in the field were also examined with 12 parallel field sections per event and compared with DT controls in the same field. To obtain the phenotypic characteristics of these plants, the following matrices were collected and expressed as the mean of each event (as well as wild-type plants) grown in the greenhouse (see Table 15) and in the field (see Table 16). There is also a column with averaging over all events per attribute.

Table 15 Phenotypic data for greenhouse grown pAt.Erl1:Gm.FT2a and DT plants.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 46.1 32.6 40.0 32.3 34.0 29.3 28.2 31.8 DOR7 115.1 99.0 109.7 99.0 99.0 93.0 91.7 101.7 PDR1R7 69.0 66.4 69.7 66.7 65.0 63.7 63.5 69.8 BRPP 23.5 7.4 16.0 6.0 9.7 1.3 4.3 7.3 NDBR 277.6 80.8 215.7 51.7 139.3 3.3 18.0 56.7 NDMS 29.8 32.3 30.7 33.7 32.7 30.7 32.7 33.3 NDPL 307.4 113.0 246.3 85.3 172.0 34.0 50.7 90.0 PDPP 605.8 346.4 447.3 349.7 493.7 240.7 194.7 352.3 PODBR 503.1 173.7 332.3 164.7 323.3 8.3 42.7 171.0 PODMS 103.0 172.7 115.0 185.0 170.3 232.3 152.0 181.3 Bobov/
node 1.9 4.0 2.1 4.1 2.9 7.1 3.8 4.0 SDPPR8 1290.0 747.5 1129.0 603.5 881.0 577.0 432.3 862.3 SW1000
(grams) 157.6 157.3 187.7 142.6 173.8 144.3 144.9 150.3

Table 16. Phenotypic data for pAt.Erl1:Gm.FT2a plants and DT plants grown in the field.

DT Average Event 1 Event 2 Event 4 Event 6 DOFR1 37.0 27.4 32.3 26.7 25.3 25.5 DOR8 114.9 110.6 113.8 110.4 107.9 110.5 PDR1R8 77.9 83.2 81.4 83.8 82.7 85.0 BRPPR8 2.1 0.8 1.5 1.2 0.3 0.3 NDBRR8 8.0 3.2 5.6 5.2 1.0 0.9 NDMSR8 19.8 19.8 19.5 19.1 19.5 21.1 NDPLR8 27.8 23.0 25.1 24.4 20.5 22.0 PDPPR8 56.1 57.8 57.4 60.6 53.1 60.2 PDMSR8 47.9 53.8 50.9 53.0 52.2 59.2 TPBR8 8.3 4.0 6.6 7.4 0.9 1.0 Bobs/Knot 2.0 2.5 2.3 2.6 2.6 2.7 SDARR8 344.3 349.9 374.1 338.4 317.8 369.2 SW1000
(oz) 5.4 5.4 5.4 5.3 5.3 5.5

[186] Transgenic soybean plants expressing the pAt.Erl1::Gm.FT2a construct flowered earlier than wild-type controls and had an increased number of beans per node (similar to plants expressing the Gm.FT2a transgene driven by the pAt.Erecta promoter). In fact, soybean plants expressing pAt.Erl1::Gm.FT2a had several phenotypes similar to pAt.Erecta::Gm.FT2a transgenic plants, including fewer days to flowering (DOFR1), fewer days to R7 ( DOR7), fewer branches per plant (BRPP), fewer nodes per plant (NDPL), fewer pods per plant (PDPP), along with an increase in pods per node (Table 15) compared to wild type controls. However, several phenotypes observed in pAt.Erecta::Gm.FT2a plants , such as number of pods per main stem (PODMS), number of pods per branch (PODBR), and 1000 seed weight (SW1000), were less pronounced in expressing pAt.Erl1 ::Gm.FT2a transgenic plants. Early results in the second year of field trials also showed early flowering, indicating similar generative traits. As a consequence, measurements for these traits can be abbreviated differently, e.g. PDPP or PODPP for pods per plant, PDMS or PODMS for pods per main stem, PDBR or PODBR for pods per branch, TPBR for total pods per plant. branches, etc.

[187] The expression profile for the Arabidopsis Erecta-like 1 ( pAt.Erl1 ) promoter in soybean, as measured by GUS staining, is more limited than the soybean pAt.Erecta expression profile described above. pAt.Erl1 controls GUS expression in vegetative axillary meristems and early floral meristems derived from axillary tissue. However, GUS staining is not observed in the shoot apical meristem at any stage at which it can be distinguished from other meristematic tissues of the developing plant. Expression of the GUS reporter under the control of the pAt.Erl1 promoter is not observed in leaf tissue, stem, or roots at any stage (data not shown). Considering that FT expression under the control of the pAt.Erecta or pAt.Erl1 promoter induced early flowering and an increase in the number of pods per node, vegetative expression of the FT transgene in or near the plant meristem as a whole may be sufficient to induce these generative and yield-related phenotypes or signs.

Example 9 Expression of miRNA targeting Gm.FT2a for suppression

[188] It has been suggested that reducing the expression level of the Gm.FT2a transgene by controlling the vegetative stage promoter in generative or floral tissues through RNA suppression may prolong the generative stage in soybean plants. To investigate whether suppression of endogenous Gm.FT2a affects the generative or morphological phenotypes of soybean plants, a transformation vector was constructed containing a DNA sequence suitable for transcription encoding a microRNA molecule (microRNA-FT2a; SEQ ID NO: 67) that targets the expression of endogenous Gm .FT2a under the control of a late vegetative and/or generative stage promoter, the pAt.AP1 promoter (SEQ ID NO: 49) or the pSl.MADS-RIN promoter (SEQ ID NO: 72), and was used to transform soybean plants by Agrobacterium -mediated transformation . Transgenic plants created by these events were studied in a greenhouse under conditions of 14-14.5-hour daylight photoperiod. Six plants per event were examined and compared on average with wild-type (WT) control plants. Averaged phenotypic data obtained from pAP1::miRNA-FT plants and wild-type plants in the greenhouse are shown in Table 17. Table 18 shows the phenotypic observations for pMADS-RIN::miRNA-FT plants and wild-type plants in the greenhouse and are expressed as averaged over each event and averaged over events and wild-type plants.

Table 17. Phenotypic data for pAP1::miRNA-FT2a plants and greenhouse grown DT plants.

DT pAP1::miRNA-FT DOFR1 40 37 DOR7 89 89 PDR1R7 49 52

[189] Under greenhouse conditions, the timing and onset of flowering in plants expressing the pAP1::miRNA-FT2a construct was approximately the same as in DT control plants. Transgenic plants expressing the pAP1::miRNA-FT2a or pMADS-RIN::miRNA-FT2a construct were also grown in the field. Six plants containing four different events of the pAP1::miRNA-FT2a construct were examined and compared on average with wild type (WT) control plants. In the case of field experiments, measurements were taken at collection (R8 step) unless otherwise noted. SDARR8 represents the number of seeds per area (ie, per square foot) at stage R8. Averaged phenotypic data from field grown plants are shown in Table 18 for the pAP1::miRNA-FT2a construct and in Table 20 for the pMADS-RIN::miRNA-FT2a construct .

Table 18. Phenotypic data for field grown pAP1::miRNA-FT2a plants and DT plants.

DT Average Event 1 Event 2 Event 3 Event 4 DOFR1 42.4 44.7 46.7 41.0 43.7 47.2 DOR8 110.7 114.5 115.0 111.6 114.4 116.8 PDR1R8 65.7 67.0 64.5 67.7 68.8 66.8 BRPPR8 2.6 2.7 2.7 2.7 2.7 2.7 NDBRR8 9.7 11.0 10.9 10.7 10.5 11.9 NDMSR8 18.3 19.3 19.5 18.4 19.3 19.9 NDPLR8 28.0 30.3 30.4 29.2 29.7 31.8 PDPPR8 44.2 39.7 37.2 43.2 40.5 37.8 PODMSR8 34.7 31.7 30.4 33.5 32.5 30.5 TPBR8 9.5 8.0 6.8 9.8 8.0 7.3 Bobs/Knot 1.6 1.4 1.3 1.5 1.4 1.2 SDARR8 282.7 251.6 247.1 259.0 264.8 235.5 SW1000
(oz) 5.1 5.2 5.2 5.1 5.2 5.2

[190] In the field, soybean plants transformed with the pAP1::miRNA-FT2a construct again had, on average, approximately the same flowering and generative traits as WT control plants, although the pAP1::miRNA-FT2a transgenic plants might have slightly less beans (PDPPR8, PDMSR8, TPBR8, bean/node) compared to DT control plants at the R8 stage.

Example 10 Modification of the Gm.FT2a expression of artificial miRNA driven by the pAP1 promoter alters generative phenotypes under greenhouse conditions.

[191] To show that suppression of Gm.FT2a in late vegetative and/or generative tissues after initial ectopic expression of transgenic Gm.FT2a can prolong the generative stage in soybean plants and/or counteract early termination, a transformation vector was constructed containing Gm.FT2a under control of the meristem-preferred vegetative stage promoter pAt.Erecta (SEQ ID NO: 31) with the Apx or Lhcb2 terminator region (T-Apx and T-Lhcb2), and a DNA molecule suitable for transcription encoding an artificial microRNA (SEQ ID NO: 67) targeted to Gm.FT2a for suppression and under the control of the pAt.AP1 promoter (SEQ ID NO: 49) with a GAPDH terminator region (each construct designated pAt.Erecta::Gm.FT2a | pAP1::miRNA-FT2a ) and used for transformation soybeans through Agrobacterium -mediated transformation. The transgenic plants generated by these events were studied in a greenhouse with a 14-14.5 hour photoperiod. For each pAt.Erecta::Gm.FT2a | pAP1::miRNA-FT2a examined six events (6 plants per event) and averaged the data. The average data for each construct was also averaged across events. Six wild-type (WT) control plants were also examined and averaged as controls. Tables 19 and 20 show the phenotypic data obtained for six events, as well as the average values for plants pAt.Erecta::Gm.FT2a | pAP1::miRNA-FT2a and DT with two different terminator regions (T-Apx and T-Lhcb2).

Table 19. Phenotypic data for plants pAt.Erecta::Gm.FT2a::T-Apx | pAP1::miRNA-FT2a::T-GAPDH and DT plants in greenhouse conditions.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 33.5 28.5 -- 30.0 28.0 28.0 28.0 28.3 DOR7 106.9 99.7 106.8 98.8 98.3 91.3 102.7 100.3 PDR1R7 76.5 70.1 -- 68.8 70.3 65.0 74.2 72.0 BRPP 20.1 13.0 20.3 16.7 13.0 6.7 9.7 12.0 FNBR 190.6 160.2 231.5 192.7 155.3 89.7 147.0 145.0 FNPL 214.6 191.5 263.8 226.7 184.3 122.0 176.0 176.3 FNST 24.0 31.3 32.3 34.0 29.0 32.3 29.0 31.3 NDBR 211.4 177.1 268.8 210.0 170.0 90.0 168.3 155.3 NDMS 33.4 36.3 38.0 37.3 36.3 34.0 35.7 36.7 NDPL 244.9 213.4 306.8 247.3 206.3 124.0 204.0 192.0 PDPP 575.8 647.5 642.8 732.0 645.8 569.7 653.7 641.0 PFNB 90.4 92.7 87.4 92.1 91.6 99.7 91.3 93.9 PFNN 87.8 91.3 86.9 92.0 89.7 98.4 89.0 91.9 PFNS 71.4 86.0 84.9 91.7 80.4 95.3 78.8 84.7 PODBR 487.3 486.2 519.8 567.3 497.3 368.3 493.3 471.3 PODMS 88.4 161.3 123.0 164.7 148.5 201.3 160.3 169.7 Bobov/
knot 2.4 3.3 2.2 3.0 3.2 4.7 3.6 3.4 SDPP8 1319.6 1197.2 1508.0 1163.0 1184.8 750.8 1170.0 1406.8 SW1000
(grams) 146.0 158.8 149.2 169.9 144.3 172.6 163.5 153.2

Table 20. Phenotypic data for pAt.Erecta::Gm.FT2a::T-Lhcb2 | pAP1::miRNA-FT2a::T-GAPDH and DT plants in greenhouse conditions.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 33.5 28.2 28 -- 28 28 29.2 28 DOR7 106.9 99.5 98.3 95.8 101 99.8 99.5 102.7 PDR1R7 76.5 71.7 68.6 -- 73 71.8 70.3 74.7 BRPP 20.1 9 5.8 8.5 8.5 6.3 fifteen 10.3 FNBR 190.6 97.8 79.8 45.5 92 84.8 149.8 135.3 FNPL 214.6 130.5 112.3 80 126.5 117 184.3 162.8 FNST 24 32.6 32.5 34.5 34.5 32.3 34.5 27.5 NDBR 211.4 106.2 84.5 45.5 97.5 91 164 154.8 NDMS 33.4 36.9 36.5 37 37.8 35 39.3 35.8 NDPL 244.9 143.1 121 82.5 135.3 126 203.3 190.5 PDPP 575.8 558.5 543.8 454 566.8 534.8 633.5 618 PFNB 90.4 93.7 94.6 100 94.7 94.5 91.4 86.8 PFNN 87.8 92.3 94.1 96.7 93.6 93.5 90.7 85 PFNS 71.4 88.5 88.2 94.1 91.3 92.1 87.7 77.4 PODBR 487.3 329.7 309.5 157 344 304.8 446 417 PODMS 88.4 228.8 234.3 297 222.8 230 187.5 201 Bobs/Knot 2.4 4.3 4.9 5.9 4.2 4.7 3.1 3.2 SDPP8 1319.6 778.9 728.5 644.3 832 964.8 699.5 804.5 SW1000
(grams) 146 155.5 158.1 182 158.7 128.4 169.8 135.8

[192] In this greenhouse experiment, transgenic soybean plants containing pAt.Erecta::Gm.FT2a | pAP1::miRNA-FT2a were characterized by an earlier onset of flowering (DOFR1) and an increased number of pods per node (and per main stem) compared to DT control plants (pods/node, PODMS). Transgenic plants with combined construct pAt.Erecta::Gm.FT2a | pAP1::miRNA-FT2a had higher plant height and increased branching, as well as increased number of nodes per plant (and per stem) and increased number of pods per plant (and per stem) compared to soybean plants with the pAt.Erecta transgene alone. ::Gm.FT2a without miRNA suppressor cassette (see, for example, Table 2). On FIG. 12 shows complete images of plants homozygous for pAt.Erecta::Gm.FT2a or pAt.Erecta::Gm.FT2a/pAP1::miRNA-FT2a demonstrating that additional suppression of the Gm.FT2a transgene is effective in mitigating early termination phenotypes. observed with a single pAt.Erecta::Gm.FT2a transgene , including low plant height and reduced branching.

[193] These data indicate that suppression of Gm.FT2a in late vegetative and/or generative tissues following an earlier ectopic dose of the Gm.FT2a transgene is effective in initiating early flowering and maintaining an increased number of pods per node, while attenuating early termination phenotypes. observed in plants containing one Gm.FT2a transgene. Interestingly, an increased number of pods per node in these plants was observed without an obvious increase in the duration of the generative stage.

Example 11 Modification of Gm.FT2a expression of artificial microRNA driven by the pAP1 promoter alters generative phenotypes in the field.

[194] Three of the four transformation events (depending on the year) of the pAt.Erecta::Gm.FT2a::T-Apx | pAP1::miRNA-FT2a::T-GAPDH described in Example 10 were grown and field tested for two consecutive years. For each of the events and control DT, phenotypic data were obtained and averaged (events are numbered for correspondence). Tables 21 and 22 show the average phenotypic data obtained for these plants, as well as the average of the three events.

[195] Four transformation events of pAt.Erecta::Gm.FT2a::T-Lhbc2 | pAP1::miRNA-FT::T-GAPDH described in Example 10 were grown and field tested for two consecutive years. For each event and DT control, phenotypic data were obtained and averaged over a number of plants. Tables 23 and 24 show the average phenotypic data obtained for these plants, as well as the average of the four events.

Table 21. Phenotypic data for plants pAt.Erecta::Gm.FT2a::T-Apx | pAP1::miRNA-FT::T-GAPDH and DT plants grown in the field (year 1).

DT Average Event 2 Event 3 Event 4 DOFR1 42.4 32.5 35.7 31.2 30.7 DOR8 110.7 112.5 115.1 113.3 109.2 PDR1R8 65.7 78.2 76.6 80.2 77.7 BRPPR8 2.6 0.9 1.1 1.0 0.5 NDBRR8 9.7 2.6 3.7 2.9 1.1 NDMSR8 18.3 19.7 20.1 19.8 19.2 NDPLR8 28.0 22.3 23.8 22.8 20.2 PDPPR8 44.2 45.8 43.6 45.6 48.1 PDMSR8 34.7 43.7 40.8 43.4 47.0 TPBR8 9.5 2.0 2.8 2.2 1.1 Bobs/Knot 1.6 2.1 1.8 2.0 2.4 SDARR8 282.7 268.6 259.1 288.7 257.9 SW1000
(oz) 5.1 4.8 4.9 4.7 4.7

Table 22. Phenotypic data for plants pAt.Erecta::Gm.FT2a::T-Apx | pAP1::miRNA-FT::T-GAPDH and DT plants grown in the field (year 2).

DT Average Event 2 Event 3 Event 4 Event 6 DOFR1 37.0 30.6 32.6 32.0 28.3 29.3 DOR8 114.9 117.6 120.7 118.9 116.0 114.8 PDR1R8 77.9 87.0 88.1 86.9 87.7 85.5 BRPPR8 2.1 0.9 1.2 2.0 0.2 0.3 NDBRR8 8.0 4.7 7.5 8.1 1.5 1.5 NDMSR8 19.8 20.8 21.4 20.4 20.1 21.1 NDPLR8 27.8 25.4 28.9 28.6 21.6 22.6 PDPPR8 56.1 64.2 65.5 66.0 63.2 62.2 PDMSR8 47.9 58.3 55.3 56.3 61.1 60.5 TPBR8 8.3 5.9 10.2 9.7 2.0 1.6 Bobs/Knot 2.0 2.6 2.3 2.2 3.0 2.8 SDARR8 344.3 365.1 359.5 380.9 340.6 379.5 SW1000
(oz) 5.4 5.4 5.4 5.6 5.4 5.4

Table 23. Phenotypic data for plants pAt.Erecta::Gm.FT2a::T- Lhbc2 | pAP1::miRNA-FT::T-GAPDH and DT plants grown in the field (year 1).

DT Average Event 1 Event 2 Event 3 Event 4 DOFR1 42.4 31.8 31.2 30.8 33.0 32.3 DOR8 110.7 110.2 110.6 109.5 111.3 109.2 PDR1R8 65.7 76.2 77.3 77.7 76.3 73.5 BRPPR8 2.6 0.6 0.4 0.4 0.6 1.0 NDBRR8 9.7 1.0 0.4 0.3 1.8 1.5 NDMSR8 18.3 19.6 19.9 19.6 19.4 19.6 NDPLR8 28.0 20.7 20.2 20.0 21.2 21.2 PDPPR8 44.2 49.5 49.0 49.8 48.8 50.5 PODMSR8 34.7 48.4 48.5 49.2 46.9 48.8 TPBR8 9.5 1.2 0.5 0.6 1.9 1.7 Bobov/
knot 1.6 2.4 2.4 2.5 2.4 2.3 SDARR8 282.7 287.0 285.6 288.6 272.7 300.9 SW1000
(oz) 5.1 4.9 5.0 4.9 4.9 4.6

Table 24. Phenotypic data for plants pAt.Erecta::Gm.FT2a::T- Lhbc2 | pAP1::miRNA-FT::T-GAPDH and DT plants grown in the field (year 2).

DT Average Event 1 Event 2 Event 3 Event 4 DOFR1 37.0 28.7 28.4 28.5 28.6 29.3 DOR8 114.9 114.6 114.7 113.7 115.0 114.9 PDR1R8 77.9 85.9 86.3 85.2 86.4 85.6 BRPPR8 2.1 0.7 0.3 0.3 0.6 1.3 NDBRR8 8.0 1.8 0.8 1.2 1.4 3.8 NDMSR8 19.8 21.7 21.9 21.2 22.0 21.5 NDPLR8 27.8 23.5 22.9 22.5 23.4 25.2 PDPPR8 56.1 63.6 60.6 68.3 59.4 66.0 PDMSR8 47.9 60.9 58.8 65.5 58.0 61.2 TPBR8 8.3 2.1 1.5 1.3 1.4 4.3 Bobs/Knot 2.0 2.7 2.6 3.1 2.5 2.7 SDARR8 344.3 358.9 316.1 355.2 365.5 398.6 SW1000
(oz) 5.4 5.5 5.6 5.6 5.5 5.2

[196] Soybean plants containing any of the constructs pAt.Erecta::Gm.FT2a | pAP1::miRNA-FT2a (with any terminator) flowered earlier (DOFR1) and had a slightly extended generative stage (in days) between R1 and R8 stages (PDR1R8) and an increased number of pods per node (and per plant) at the R8 stage (PDPPR8 , bean/node) when grown in the field compared to control DT plants. However, soybean plants containing pAt.Erecta::Gm.FT2a | pAP1::miRNA-FT2a::T-GAPDH were also characterized by reduced branching (BRPPR8) compared to DT control plants. Early results for the third year of field trials of plants with pAt.Erecta::Gm.FT2a | pAP1::miRNA-FT2a::T-GAPDH also showed early flowering, indicating similar generative traits.

[197] These field results show that both pAt.Erecta::Gm.FT2a | pAP1::miRNA-FT2a::T-GAPDH with different terminators are able to increase yield traits and alleviate early termination phenotypes in the field (see e.g. Example 3 above), which is consistent with the greenhouse data shown in Example 10 above. These data further support the model that suppression of the Gm.FT2a transgene helps to reduce or mitigate early termination and plant architecture phenotypes, including reduction in plant height, branching, and number of nodes per plant (or main stem), compared with expression of the Gm.FT2a transgene alone. .

Example 12 Modification of Gm.FT2a expression of artificial miRNA driven by the pSl.MADS5 promoter alters generative phenotypes under greenhouse conditions.

[198] In addition to the pAP1 promoter, other generative stage promoters were used to express a miRNA molecule targeted at the Gm.FT2a transgene for suppression. In one set of experiments, a transformation vector was constructed containing Gm.FT2a under the control of the pAt.Erecta promoter and a transcriptionable DNA sequence encoding an artificial miRNA that targets Gm. FT2a was suppressed and driven by the Solanum lycopersicum MADS5 promoter ( pSl.MADS5; SEQ ID NO: 71) and was used to transform soybeans by Agrobacterium -mediated transformation. The transgenic plants generated by these events were studied for their phenotypes in a greenhouse with a 14-14.5 hour photoperiod. For the construction pAt.Erecta::Gm.FT2a::T-Lhcb2 | pSl.MADS5::miRNA-FT2a::T-GAPDH was examined for six events (6 plants per event). Six plants were also examined and averaged over wild-type control (WT) plants.

[199] Table 25 shows the average phenotypic data for each event and DT plant and the average value for each trait across all events studied. ("--" means no data received)

Table 25. Phenotypic data for plants pAt.Erecta::Gm.FT2a::T-Lhcb2 | pSl.MADS5::miRNA-FT::T-GAPDH and DT plants under greenhouse conditions.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 33.5 28.0 28.0 -- -- -- -- 28.0 DOR7 106.9 87.1 83.3 86.7 89.7 87.5 88.3 87.2 PDR1R7 76.5 58.5 56.0 -- -- -- -- 61.0 BRPP 20.1 2.1 1.0 1.5 -- 4.0 2.0 2.0 FNBR 190.6 7.9 3.0 5.0 -- 16.5 7.7 7.3 FNPL 214.6 29.6 11.0 33.5 31.5 41.8 24.3 35.5 FNST 24.0 25.8 10.3 31.0 31.5 33.5 18.5 30.0 NDBR 211.4 8.4 3.0 6.0 -- 17.0 8.0 8.0 NDMS 33.4 26.6 10.8 31.5 32.3 33.8 19.8 31.5 NDPL 244.9 30.6 11.5 34.5 32.3 42.3 25.8 37.5 PDPP 575.8 252.4 34.8 244.0 265.3 253.3 157.8 559.5 PFNB 90.4 94.6 100.0 87.5 -- 98.1 95.2 92.1 PFNN 87.8 96.6 95.8 97.5 97.8 99.0 94.9 94.9 PFNS 71.4 96.8 95.8 98.5 97.8 99.2 94.5 95.1 PODBR 487.3 12.0 3.0 5.5 -- 33.0 9.7 8.7 PODMS 88.4 246.8 34.0 241.3 265.3 236.8 150.5 553.0 Bobs/Knot 2.4 7.6 3.1 7.1 8.2 6.3 6.4 14.6 SDPP8 1319.6 335.3 38.0 321.3 359.3 527.8 305.8 460.0 SW1000
(grams) 146.0 178.6 180.8 167.2 173.0 183.3 182.3 185.0

[200] Similar to previous examples, restriction of ectopic expression of Gm.FT2a with artificial miRNA expressed in generative tissues resulted in soybean plants that flowered earlier (DOFR1) than control DT plants. However, transgenic soybean plants containing the construct pAt.Erecta::Gm.FT2a::T-Lhcb2 | pSl.MADS5::miRNA-FT2a::T-GAPDH, had fewer pods per plant (PDPP), fewer nodes per plant (NDPL), reduced number of branches (BRPP), fewer seeds per plant at the R8 stage (SDPP8) and less duration of the generative stage between R1 and R7 stages (PDR1R7) compared to control DT plants, although these transgenic plants had a higher number of pods per node (and per main stem) compared to control DT plants (pods/node, PODMS) and phenotypes with improved plant architecture compared to the expression of one pAt.Erecta::Gm.FT2a (see, for example, table 2).

[201] These data further support the model that suppression of the Gm.FT2a transgene helps to reduce or alleviate early termination phenotypes with a single Gm.FT2a transgene, but miRNA-FT2a expression with the pMADS5 promoter may not be as efficient as with the pAP1 promoter, in regarding the mitigation of early termination phenotypes.

Example 13 Modification of Gm.FT2a expression of artificial microRNA driven by the pSl.MADS5 promoter alters generative phenotypes in the field.

[202] Transgenic plants containing three or four transformation events of the construct pAt.Erecta::Gm.FT2a | pSl.MADS5::miRNA-FT2a described in Example 12 were grown and field tested for two consecutive years. For each event and DT control, data were obtained for six plants (events are numbered for consistency). Tables 26 and 27 show the average phenotypic data obtained for each event and TD control and the average value for each trait across all events.

Table 26. Phenotypic data for plants pAt.Erecta::Gm.FT2a::T-Lhcb2 | pSl.MADS5::miRNA-FT2a::T-GAPDH and DT plants grown in the field (year 1).

DT Average Event 2 Event 3 Event 4 DOFR1 42.4 27.6 27.7 27.5 27.7 DOR8 110.7 100.8 100.3 101.8 100.2 PDR1R8 65.7 73.1 73.2 74.0 72.0 BRPPR8 2.6 0.1 0.1 0.0 0.1 NDBRR8 9.7 0.2 0.2 0.1 0.3 NDMSR8 18.3 15.2 14.5 15.7 15.3 NDPLR8 28.0 15.4 14.7 15.8 15.6 PDPPR8 44.2 43.2 41.7 45.4 42.5 PDMSR8 34.7 43.0 41.4 45.3 42.2 TPBR8 9.5 0.2 0.3 0.0 0.3 Bobs/Knot 1.6 2.9 2.9 2.9 2.8 SDARR8 282.7 243.7 233.8 264.4 233.0 SW1000
(oz) 5.1 4.6 4.4 4.7 4.7

Table 27. Phenotypic data for pAt.Erecta::Gm.FT2a::T-Lhcb2 | pSlLe.MADS5::miRNA-FT2a::T-GAPDH and DT plants grown in the field (year 2).

DT Average Event 2 Event 3 Event 4 Event 5 DOFR1 37.0 24.5 24.3 24.7 24.3 24.8 DOR8 114.9 106.7 106.3 106.8 106.2 107.7 PDR1R8 77.9 82.2 82.1 82.1 81.8 82.9 BRPPR8 2.1 0.3 0.3 0.2 0.5 0.2 NDBRR8 8.0 1.1 0.8 0.8 2.0 0.7 NDMSR8 19.8 15.2 15.4 15.3 15.4 14.7 NDPLR8 27.8 16.2 16.2 16.1 17.3 15.1 PDPPR8 56.1 45.7 46.4 48.9 50.1 37.4 PDMSR8 47.9 44.0 45.2 47.6 46.3 37.0 TPBR8 8.3 1.8 1.2 1.4 3.8 1.0 Bobs/Knot 2.0 2.8 2.9 3.0 2.9 2.5 SDARR8 344.3 254.9 280.4 236.5 267.9 235.0 SW1000
(oz) 5.4 5.3 5.1 5.5 5.4 5.0

[203] Soybean plants containing the construct pAt.Erecta::Gm.FT2a | pSl.MADS5::miRNA-FT2a , flowered earlier (DOFR1) and had a slightly extended generative stage (in days) between R1 and R8 stages (PDR1R8) and an increased number of pods per node at the R8 stage (pods/node) in the field according to compared with control plants DT. However, soybean plants containing pAt.Erecta::Gm.FT2a | pSl.MADS5::miRNA-FT2a were also characterized by reduced branching (BRPPR8) compared to control DT plants.

Example 14 Modification of Gm.FT2a expression of artificial microRNA driven by the pSl.NOD promoter alters generative phenotypes under greenhouse conditions.

[204] The third promoter ( pSl.NOD ), which regulates the expression of microRNA-FT2a, was also investigated in greenhouse conditions. A transformation vector was constructed containing Gm.FT2a under the control of the pAt.Erecta promoter (SEQ ID NO: 31) and a DNA sequence suitable for transcription encoding miRNA-FT2a (SEQ ID NO: 67) under the control of the Solanum lycopersicum NOD promoter ( pSl.NOD; SEQ ID NO: 70) and used to transform soybean by Agrobacterium -mediated transformation. Transgenic plants containing six events of this construct pAt.Erecta::Gm.FT2a | pSl.NOD::miRNA-FT2a (six plants per event) were grown in a greenhouse with a 14-14.5 hour photoperiod. Data were obtained for six plants for each event and wild type control (WT). Table 28 shows the average phenotypic data obtained for each event and control DT and the average value for each trait across all events.

Table 28. Phenotypic data for greenhouse grown pAt.Erecta::Gm.FT2a | pSl.NOD :: miRNA-FT2a and plant DT.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 40.0 20.4 20.3 20.4 20.8 20.7 20.0 20.2 DOR7 107.3 90.9 87.2 86.0 96.8 86.0 86.0 99.6 PDR1R7 67.3 70.5 66.8 65.6 76.0 65.4 66.0 79.4 BRPP 17.9 3.3 -- 2.0 7.7 1.5 2.0 -- FNBR 182.3 34.3 -- 19.0 106.0 4.5 7.5 -- FNPL 205.9 44.2 20.0 27.7 132.3 20.8 21.0 19.0 FNST 23.6 20.2 20.0 21.3 26.3 18.5 16.0 19.0 NDBR 231.0 37.3 -- 20.0 114.0 5.5 9.5 -- NDMS 34.4 22.6 21.3 22.3 31.0 20.3 18.7 20.7 NDPL 265.4 48.5 21.3 29.0 145.0 23.0 25.0 20.7 PDPP 703.0 228.3 133.3 188.0 563.7 108.0 139.7 142.3 PFNB 78.9 85.6 -- 95.0 93.3 75.0 78.9 -- PFNN 77.5 90.8 94.3 96.1 91.3 90.3 84.5 91.8 PFNS 69.8 89.7 94.3 95.2 84.8 91.1 85.8 91.8 PODBR 584.8 126.5 -- 100.0 390.3 5.0 10.5 -- PODMS 115.1 141.7 133.3 154.7 173.3 105.5 132.7 142.3 Bobs/Knot 2.7 5.6 6.3 6.5 3.9 4.8 5.9 6.8 SDPP8 1413.0 437.0 307.5 409.7 973.0 243.5 282.7 276.3 SW1000
(grams) 126.2 113.4 120.5 117.5 83.6 137.5 118.4 109.8

[205] Soybean plants containing the construct pAt.Erecta::Gm.FT2a | pSl.NOD::miRNA-FT2a , flowered earlier (DOFR1) than DT control plants and had a shorter generative stage (PDR1R7), fewer branches per plant (BRPP), fewer nodes per plant (NDPL), and fewer pods per plant ( PDPP) in greenhouse compared to DT control plants. However, these transgenic plants had higher numbers of pods per node (and per main stem) compared to DT (pods/node, PODMS) plants and phenotypes with improved plant architecture compared to expression of pAt.Erecta::Gm.FT2a alone (see e.g. table 2).

[206] These data further support the model that suppression of the Gm.FT2a transgene helps to reduce or alleviate early termination phenotypes, but miRNA-FT2a expression under the control of the pSl.NOD promoter may not be as efficient as in the case of the pAP1 promoter in terms of alleviating early termination phenotypes, although these transgenic plants had a higher number of pods per node (and per main stem) than control DT plants (pods/node, PODMS).

Example 15 Modification of Gm.FT2a ectopic expression with a target site for heterologous miR172 alters generative phenotypes under greenhouse conditions.

[207] As an alternative approach for suppression of Gm.FT2a , constructs were created containing the Gm.FT2a transgene and an engineered target or binding site (or sensor) of microRNA, which is recognized by endogenous microRNA in soy, which served to attenuate and/or correct ectopic expression of the transgene Gm.FT2a . Transformation vectors were constructed containing the Gm.FT2a transgene driven by the pAt.Erecta promoter (SEQ ID NO: 31) with an Apx or Lhcb2 terminator region (T-Apx and T-Lhcb2), and additionally containing the sequence (SEQ ID NO: 98), miR172 microRNA target site (SEQ ID NO: 99) in the transgenic Gm.FT2a mRNA and used to transform soybean by Agrobacterium -mediated transformation. The transgenic plants generated by these events were studied for their phenotypes in a greenhouse with a 14-14.5 hour photoperiod. For each of the two pAt.Erecta::Gm.FT2a constructs with the miR172 binding site, six events were examined (6 plants per event). Six plants were also examined and averaged over wild-type control (WT) plants. Tables 29 and 30 show the average phenotypic data obtained for each event and control DT for each of the two constructs and the average value for each trait across all events studied.

Table 29. Phenotypic data for pAt.Erecta::Gm.FT2a::T-Apx / miR172 target site and DT plants.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 33.5 28.0 -- 28.0 -- -- -- -- DOR7 106.9 88.3 91.3 89.7 86.3 89.0 88.2 85.3 PDR1R7 76.5 64.5 -- 64.5 -- -- -- -- BRPP 20.1 3.9 1.5 4.0 -- 11.5 1.5 1.0 FNBR 190.6 21.6 4.0 21.3 -- 75.0 3.3 4.5 FNPL 214.6 37.0 39.0 50.3 26.5 64.3 16.5 25.5 FNST 24.0 26.8 37.0 34.3 26.5 26.8 13.3 23.3 NDBR 211.4 26.8 4.0 25.3 -- 96.5 3.5 4.5 NDMS 33.4 28.8 38.0 38.0 26.8 32.0 14.3 23.8 NDPL 244.9 41.3 40.0 57.0 26.8 80.3 17.8 26.0 PDPP 575.8 227.9 294.0 331.5 201.8 297.0 68.0 175.3 PFNB 90.4 93.1 100.0 83.7 -- 88.3 93.8 100.0 PFNN 87.8 95.0 97.6 91.5 99.1 90.7 93.5 97.9 PFNS 71.4 93.4 97.4 90.9 99.1 82.2 93.4 97.7 PODBR 487.3 60.8 4.5 82.0 -- 206.0 5.0 6.5 PODMS 88.4 198.8 291.8 270.0 201.8 194.0 63.0 172.0 Bobov/
knot 2.4 6.4 7.4 6.7 7.5 6.1 3.9 6.8 SDPP8 1319.6 330.0 443.8 460.0 368.5 409.3 47.8 250.8 SW1000
(grams) 146.0 169.5 166.8 176.3 177.7 171.8 152.7 172.1

Table 30 Phenotypic data for pAt.Erecta::Gm.FT2a::T-Lhcb2 / miR172 target site and DT plants.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 40.3 27.2 28.0 25.0 25.0 33.3 26.3 25.3 DOR7 116.5 99.9 96.6 88.0 97.0 123.0 102.4 92.7 PDR1R7 76.3 73.0 70.8 63.0 70.8 89.3 76.6 67.3 BRPP 17.5 5.3 3.0 1.3 1.5 13.5 6.3 6.3 FNBR 162.5 48.6 33.0 3.8 4.5 153.5 46.3 50.3 FNPL 185.8 65.7 69.0 24.3 32.0 128.3 70.3 70.3 FNST 23.3 27.8 36.0 20.5 27.5 26.0 24.0 32.5 NDBR 249.5 55.1 37.0 5.5 7.0 173.5 50.8 56.7 NDMS 33.5 31.9 38.3 21.8 28.5 36.7 31.8 34.3 NDPL 283.0 74.9 75.3 27.3 35.5 152.3 82.5 76.8 PDPP 628.5 317.3 407.0 132.0 198.5 433.3 338.3 394.8 PFNB 65.2 81.9 85.3 87.5 67.8 87.7 77.5 85.5 PFNN 65.7 85.3 93.8 91.6 90.2 69.2 73.9 93.2 PFNS 69.0 86.4 94.5 94.7 96.4 66.4 71.3 95.1 PODBR 504.8 158.8 131.7 8.0 8.5 478.5 150.5 175.3 PODMS 123.8 192.4 275.3 124.0 190.0 114.3 187.8 263.3 Bobs/Knot 2.2 4.8 6.3 5.1 5.6 2.2 3.6 6.2 SDPP8 1261.3 559.5 712.0 118.8 655.7 593.3 603.5 673.5 SW1000
(grams) 135.0 128.8 105.2 144.5 122.6 120.2 175.9 104.6

[208] Soybean plants containing either of the two constructspAt.Erecta::Gm.FT2a/miR172 target site, flowered earlier (DOFR1) but had reduced branching (BRPP) and fewer pods and nodes per plant (PDPP, NDPL) compared to DT control plants. However, these transgenic plants had a higher number of pods per node (and per main stem) compared to DT (bean/node, PODMS) control plants and phenotypes with improved plant architecture compared to expressing onepAt.Erecta::Gm.FT2a(see table 2).

[209] These data support the hypothesis that suppression of the Gm.FT2a transgene with a target site for endogenous miR172 is effective in reducing or alleviating the early termination phenotypes observed when the Gm.FT2a transgene is expressed, but suppressing the Gm.FT2a transgene with a target site for engineered miR172 may not be as efficient (at least in some cases) as artificial miRNA expression to suppress Gm.FT2a using a late vegetative and/or generative stage promoter such as the pAP1 promoter to mitigate early termination phenotypes, although Gm.FT2a transgenic plants containing the miR172 target site had more pods per node (and per main stem) than control DT (bean/node, PODMS) plants with phenotypes with improved plant architecture compared to Gm.FT2a alone expression . On FIG. 13 shows complete images of plants homozygous for pAt.Erecta::Gm.FT2a or pAt.Erecta::Gm.FT2a/ miR172 targeting site demonstrating that additional suppression by the miR172 targeting site is effective in mitigating the early termination phenotypes observed with one pAt.Erecta::Gm.FT2a transgene , including small plant height and reduced branching.

Example 16 Modification of Gm.FT2a ectopic expression with miR172 target site alters generative phenotypes in the field.

[210] Transgenic plants containing the four transformation events of the pAt.Erecta::Gm.FT2a::T-Apx construct/miR172 target site described in Example 15 were grown and field tested. Events 1-4 in Table 29 correspond to Events 1-4 in Table 31, respectively. Data were obtained for six plants for each DT event and control. Table 31 shows the average phenotypic data obtained for each event and control DT and the average value for each trait across all events.

Table 31. Phenotypic data for field grown pAt.Erecta::Gm.FT2a::T-Apx /miR172 target site and DT plants.

DT Average Event 1 Event 2 Event 3 Event 4 DOFR1 42.4 26.8 26.5 27 27.3 26.3 DOR8 110.7 91.9 93.7 92.5 91.3 90.1 PDR1R8 65.7 66.1 68.5 66.4 65.2 64.2 BRPPR8 2.6 0.1 0.2 0.1 0.1 0.1 NDBRR8 9.7 0.3 0.5 0.2 0.3 0.3 NDMSR8 18.3 10.8 12.5 11.2 10.7 8.9 NDPLR8 28.0 11.1 13 11.3 11.0 9.1 PDPPR8 44.2 23.2 30.1 25.0 21.7 15.8 PDMSR8 34.7 22.7 29.4 24.7 21.3 15.3 TPBR8 9.5 0.5 0.7 0.2 0.4 0.5 Bobs/Knot 1.6 2.1 2.4 2.1 2.0 1.7 SDARR8 282.7 130.3 148.7 155.3 129.3 88.1 SW1000 (oz) 5.1 4.6 4.7 4.5 4.6 4.6

[211] Similar to greenhouse data, soybean plants with construct pAt.Erecta::Gm.FT2a::T-Apx / miR172 target site flowered earlier (DOFR1) and had more pods per node (and per main stem) at the R8 stage ( PDPPR8, pod/node) in the field compared to DT control plants. However, these pAt.Erecta::Gm.FT2a::T-Apx /miR172 construct soybean plants also had reduced branching (BRPPR8) and reduced number of pods and nodes per plant (PDPPR8, NDPLR8) compared to DT control plants.

Example 17 Modification of Gm.FT2a ectopic expression with a truncated pAt.Erecta promoter and a heterologous miR172 target site altered generative phenotypes under greenhouse conditions.

[212] The truncated pAt.Erecta promoter was used to drive attenuated expression of the Gm.FT2a transgene and fused to the miR172 miRNA target site to possibly further attenuate and/or correct ectopic expression of the Gm.FT2a transgene. Transformation vectors were constructed containing the Gm.FT2a transgene driven by the truncated pAt.Erecta promoter (SEQ ID NO: 32) with the Lhcb2 terminator region (T-Lhcb2), and additionally containing the sequence (SEQ ID NO: 98) encoding the miR172 target site ( SEQ ID NO: 99) and used to transform soybean plants via Agrobacterium -mediated transformation. The transgenic plants generated by these events were studied for their phenotypes in a greenhouse with a 14-14.5 hour photoperiod. For the pAt.Erecta_truncated::Gm.FT2a construct with the miR172 target site, six events were examined (6 plants per event). Six plants were also examined and averaged over wild-type control (WT) plants. Table 32 shows the average phenotypic data obtained for each event and control of TD and the average value for each trait for all events studied.

Table 32. Phenotypic data for plants pErecta_truncated::Gm.FT2a::T-Lhbc2 /miR172 target site and DT plants.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 40.3 33.4 32.5 25.0 34.7 35.7 38.7 33.8 DOR7 116.5 107.1 105.5 92.2 109.4 107.7 116.8 111.3 PDR1R7 76.3 74.1 73.0 67.0 75.0 72.0 80.0 77.6 BRPP 17.5 14.2 15.3 2.0 14.8 15.8 20.8 16.8 FNBR 162.5 148.3 171.3 5.7 180.5 156.8 177.3 198.5 FNPL 185.8 175.0 201.3 36.5 207.0 180.0 203.0 222.3 FNST 23.3 26.9 30.0 32.3 26.5 23.3 25.8 23.8 NDBR 249.5 211.9 220.8 5.7 256.0 258.0 281.8 249.5 NDMS 33.5 38.2 39.0 33.3 36.0 35.0 48.3 37.8 NDPL 283.0 249.9 259.8 37.5 292.0 293.0 330.0 287.3 PDPP 628.5 635.0 752.5 324.8 727.8 615.0 676.3 713.8 PFNB 65.2 75.7 77.8 100.0 70.1 62.3 63.0 81.0 PFNN 65.7 74.8 77.8 97.5 70.5 62.8 61.5 78.5 PFNS 69.0 72.1 78.0 97.0 74.3 67.2 53.0 63.2 PODBR 504.8 469.6 577.3 12.7 581.5 508.0 531.5 606.8 PODMS 123.8 165.9 175.3 315.3 146.3 107.0 144.8 107.0 Bobs/Knot 2.2 3.5 2.9 8.7 2.5 2.1 2.1 2.6 SDPP8 1261.3 1144.0 1550.3 691.5 1053.8 984.0 1107.3 1477.3 SW1000
(grams) 135.0 114.1 126.5 116.6 96.0 98.0 115.3 131.9

[213] Soybean plants containing the pAt.Erecta_truncated::Gm.FT2a/ miR172 target site flowered earlier (DOFR1) but had slightly reduced branching (BRPP) and fewer nodes per plant (NDPL) compared to DT control plants . However, these transgenic plants had higher numbers of pods per node (and per main stem) compared to DT (pods/node, PODMS) control plants and phenotypes with improved plant architecture compared to expression of pAt.Erecta::Gm.FT2a alone (see , for example, table 2).

[214] These data support the hypothesis that suppression of the Gm.FT2a transgene by a target site for endogenous miR172 is effective in reducing or alleviating the early termination observed when the Gm.FT2a transgene is expressed, and/or that expression of the Gm.FT2a transgene with a truncated promoter pAt.Erecta can further alleviate early termination phenotypes for single Gm.FT2a expression.

Example 18 Modification of Gm.FT2a ectopic expression with the truncated pAt.Erecta promoter and miR172 target site alters generative phenotypes in the field.

[215] Transgenic plants for the three events of the construct pAt.Erecta_truncated::Gm.FT2a::T-Lhbc2 / miR172 target site described in Example 18 were grown and field tested. Events 1, 2, and 5 in Table 32 correspond to Events 1-3 in Table 33, respectively. Data were obtained for six plants for each DT event and control. Table 33 shows the average phenotypic data for each event and control of TD and the average value for each trait across all events.

Table 33. Phenotypic data for pErecta_truncated::Gm.FT2a::T-Lhbc2 / miR172 target site and DT plants in the field.

DT Average Event 1 Event 2 Event 3 DOFR1 42.4 30.4 29.8 27.7 33.8 DOR8 110.7 105.3 108.1 99.8 108.0 PDR1R8 65.7 74.3 77.0 73.8 72.2 BRPPR8 2.6 0.7 1.0 0.0 1.1 NDBRR8 9.7 1.6 1.5 0.2 3.0 NDMSR8 18.3 17.3 17.8 14.8 19.2 NDPLR8 28.0 18.8 19.3 15.0 22.2 PDPPR8 44.2 43.9 45.5 41.4 44.7 PDMSR8 34.7 42.4 43.9 41.1 42.1 TPBR8 9.5 1.6 1.6 0.4 2.7 Bobs/Knot 1.6 2.4 2.4 2.7 2.1 SDARR8 282.7 263.4 278.2 242.7 269.2 SW1000
(oz) 5.1 4.9 5.0 4.5 5.1

[216] Similar to greenhouse data, soybean plants with construct pAt.Erecta_truncated::Gm.FT2a / miR172 target site flowered earlier (DOFR1) and had more pods per node (and per main stem) at the R8 stage (PDMSR8, pods/node ) in the field compared to control DT plants. However, these soybean plants with construct pAt.Erecta_truncated::Gm.FT2a /miR172 target site also had reduced branching (BRPPR8) and reduced number of pods and nodes per plant (NDPLR8) compared to DT control plants.

Example 19 Modification of ectopic expression of Gm.FT2a and native expression of Gm.FT2a , Gm.FT5a and Gm.FT5b in soybean with artificial miRNA alters generative phenotypes.

[217] A transformation vector was constructed and used to transform soybean plants by Agrobacterium -mediated transformation, the vector containing the Gm.FT2a transgene as described above under the control of the meristem-preferred vegetative stage promoter pAt.Erecta (SEQ ID NO: 31) with terminator region Lhcb2 (T-Lhcb2), and a DNA sequence suitable for transcription encoding an artificial miRNA (SEQ ID NO: 102) that targets not only Gm.FT2a (ectopic and endogenous), but also endogenous Gm.FT5a and Gm.FT5b for suppression, while the DNA sequence suitable for transcription, encoding microRNA, is under the control of the pAt.AP1 promoter (SEQ ID NO: 49) with the GAPDH terminator region. Transgenic plants created by these events were studied in a greenhouse under conditions of 14-14.5-hour daylight photoperiod. Data were obtained for six plants for each DT event and control. Table 34 shows the average phenotypic data obtained for each event and control DT and the average value for each trait across all events.

Table 34. Phenotypic data for plants pAt.Erecta::Gm.FT2a::T-Lhbc2 | pAP1::miRNA-FT2a/FT5a/FT5b::T-GAPDH and greenhouse grown DT plants.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 39.3 32.2 34.0 32.0 33.0 30.3 31.7 32.3 DOR7 107.0 98.0 104.2 99.3 97.8 93.7 94.5 98.5 PDR1R7 67.7 65.8 70.2 67.3 64.8 63.3 62.8 66.2 BRPPR8 16.8 9.9 13.8 12.7 12.2 1.8 9.0 9.7 NDBRR8 225.8 60.9 64.3 72.3 93.8 9.2 55.3 70.7 NDMSR8 29.7 29.7 34.8 33.8 33.5 15.8 33.3 27.2 NDPLR8 255.5 90.7 99.2 106.2 127.3 25.0 88.7 97.8 PDMSR8 74.8 177.2 212.8 222.2 161.8 92.3 218.8 155.2 PDNDBRR8 1.8 2.6 3.2 3.4 2.6 1.8 2.6 2.2 PDNDMSR8 2.3 6.0 6.2 6.6 5.2 5.4 6.6 5.7 Bobs/Knot 1.9 4.2 4.3 4.5 3.6 4.5 4.5 4.0 PDPPR8 482.7 349.0 411.3 470.8 418.2 111.8 346.3 335.5 PHTR8 1016.3 955.5 1133.5 1156.3 1032.5 475.5 1049.3 885.8 SDPDR8 2.1 1.9 1.9 1.8 1.9 1.6 2.3 1.9 SNUM 1012.5 694.9 798.3 825.3 814.2 220.3 789.0 722.3 TPBR8 407.8 171.8 198.5 248.7 256.3 19.5 127.5 180.3 SW1000
(grams) 141.2 135.4 144.3 150.8 141.5 107.1 149.5 119.4

[218] Table 34 shows measurements of some additional traits, including PDNDBRR8 (beans per node on branches) and PDNDMSR8 (beans per node on main stem) at stage R8, PHTR8 (plant height at stage R8 in millimeters), SDPDR8 ( number of seeds per bean at R8) and SNUM (number of seeds per plant). Soybean plants expressing the pAt.Erecta::Gm.FT2a cassette with combined suppression of Gm.FT2a , Gm.FT5a and Gm.FT5b flowered earlier (DOFR1) and had a significantly increased number of pods per node (and an increased number of pods per main stem) but reduced branching compared to DT control plants.

Example 20 Expression of ectopic Gm.FT2a under the control of the pAP1 promoter changes the flowering time and the number of pods per node in soybeans.

[219] A transformation vector containing Gm.FT2a under the control of the pAt.AP1 promoter with an Lhcb2 terminator region was constructed and used to transform soybean by Agrobacterium -mediated transformation. The transgenic plants generated by these events were studied in a greenhouse with a 14-14.5 hour photoperiod. Data were obtained for six plants for each DT event and control. Table 35 shows the average phenotypic data obtained for each event and control DT and the average value for each trait across all events.

Table 35 Phenotypic data for greenhouse grown pAt.AP1::Gm.FT2a::T-Lhbc2 and DT plants.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 38.3 27.4 26.7 26.7 29.3 28.2 27.0 26.5 DOR7 106.7 94.0 91.2 92.8 96.7 93.7 94.2 95.7 PDR1R7 68.3 66.6 64.5 66.2 67.3 65.5 67.2 69.2 BRPPR8 26.2 8.5 3.3 6.3 17.2 10.0 6.7 7.7 NDBRR8 352.5 90.5 14.7 75.2 175.3 106.8 91.2 79.7 NDMSR8 31.5 31.8 28.8 34.5 34.3 31.7 27.5 33.8 NDPLR8 384.0 122.2 43.5 109.7 209.7 138.5 118.4 113.5 PDMSR8 69.3 165.8 122.5 212.3 120.7 182.0 158.7 198.3 PDNDBRR8 1.7 3.2 1.7 4.2 2.5 4.3 2.7 4.1 PDNDMSR8 2.1 5.2 4.2 6.1 3.5 6.3 5.2 5.8 Bobs/Knot 1.7 3.9 3.4 4.9 2.6 4.8 3.1 4.6 PDPPR8 645.2 433.9 148.8 513.5 544.5 485.7 394.2 517.0 PHTR8 1197.0 1153.4 1065.3 1280.5 1240.8 1177.5 961.1 1195.0 SDPDR8 2.4 2.4 2.3 2.4 2.5 2.4 2.5 2.4 SNUM 1522.7 1059.9 349.7 1221.7 1363.7 1172.8 985.3 1266.3 TPBR8 575.8 268.4 26.3 301.2 423.8 303.7 236.8 318.7 SW1000
(grams) 196.7 180.3 213.4 170.0 181.6 171.9 174.0 171.0

[220] Soybean plants expressing the pAt.AP1::Gm.FT2a::T-Lhbc2 cassette flowered earlier (DOFR1) and had a significantly increased number of beans per node (and an increased number of beans on the main stem), but reduced branching compared to with control plants DT. Early field studies with this construct also demonstrated early flowering, thus indicating that plants grown in the field will exhibit similar generative traits.

Example 21 Expression of ectopic Gm.FT2a under the control of the pSl.Nod promoter in soy.

[221] A transformation vector containing Gm.FT2a driven by the pSl.Nod promoter with an Lhcb2 terminator region was constructed and used to transform soybean by Agrobacterium -mediated transformation. The transgenic plants generated by these events were studied in a greenhouse with a 14-14.5 hour photoperiod. Data were obtained for six plants for each DT event and control. Table 36 shows the average phenotypic data obtained for each event and control DT and the average value for each trait across all events.

Table 36 Phenotypic data for pSl.Nod::Gm.FT2a::T-Lhbc2 plants and greenhouse DT plants.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 38.3 38.1 37.5 37.0 38.3 38.8 39.5 37.7 DOR7 107.8 106.4 105.2 105.0 105.5 109.2 104.7 108.8 BRPPR8 25.3 25.3 29.3 23.8 25.2 23.8 24.3 25.3 NDBRR8 312.7 337.9 373.5 315.2 336.5 333.0 317.0 352.3 NDMSR8 38.2 36.4 34.7 37.2 37.2 39.3 36.3 33.8 NDPLR8 350.8 373.3 401.8 352.3 373.7 372.3 353.3 386.2 PDMSR8 89.5 91.3 89.3 96.7 100.0 97.8 93.2 70.5 PDNDBRR8 2.0 1.8 1.8 1.8 1.8 1.9 1.8 1.7 PDNDMSR8 2.3 2.3 1.7 2.6 2.7 2.5 2.6 2.0 Bobs/Knot 2.0 1.9 2.0 1.9 1.8 1.9 1.8 1.7 PDPPR8 700.8 677.5 750.8 664.3 681.5 676.7 640.8 650.7 PDR1R7 69.5 68.3 67.7 68.0 67.2 70.3 65.2 71.2 PHTR8 1324.2 1256.6 1136.6 1220.8 1315.2 1321.7 1295.3 1249.8 SDPDR8 2.5 2.4 2.1 2.4 2.4 2.4 2.5 2.5 SNUM 1713.3 1591.3 1517.7 1584.5 1642.7 1600.8 1588.7 1613.7 TPBR8 611.3 586.2 661.5 567.7 581.5 578.8 547.7 580.2 SW1000 (grams) 187.6 188.0 197.5 187.2 189.4 185.2 187.6 181.3

[222] In these experiments, no significant changes were observed between pSl.Nod::Gm.FT2a::T-Lhbc2 plants and DT plants. Early field results with this construct further show no change at the onset of flowering.

Example 22 Modification of Gm.FT2a driven by the pAt.Erl1 promoter with an artificial microRNA driven by the pSl.MADS-RIN promoter in soy.

[223] To test the hypothesis that suppression of Gm.FT2a in late vegetative and/or generative tissues following initial ectopic expression of transgenic Gm.FT2a may prolong the generative stage in soybean plants, a transformation vector was constructed containing Gm.FT2a under the control of a meristem-preferred promoter. vegetative stage pAt.Erl1 (SEQ ID NO: 44) with a GAPDH terminator region (T-GAPDH), and a transcriptionable DNA sequence encoding an artificial miRNA (SEQ ID NO: 67) targeting Gm.FT2a for suppression and control pSl.MADS-RIN promoter (SEQ ID NO: 72) with a T-Apx terminator region and used to transform soybean by Agrobacterium -mediated transformation. The transgenic plants generated by these events were studied in a greenhouse with a 14-14.5 hour photoperiod. Data were obtained for six plants for each DT event and control. Table 37 shows the average phenotypic data obtained for each event and control DT and the average value for each trait across all events.

Table 37. Phenotypic data for pAt.Erl1::Gm.FT2a::T-GAPDH | pSl.MADS-RIN::miRNA-FT2a:: T-Apx and greenhouse grown DT plants.

DT Average Event 1 Event 2 Event 3 Event 4 Event 5 Event 6 DOFR1 39.3 33.8 33.0 34.0 35.7 35.0 33.3 31.5 DOR7 107.5 99.9 98.7 98.5 99.0 105.8 101.0 96.5 PDR1R7 68.2 66.2 65.7 64.6 63.3 70.8 67.7 65.0 BRPPR8 16.5 14.4 13.3 14.2 13.2 17.8 16.0 12.0 NDBRR8 180.2 131.8 100.3 128.8 124.5 187.0 141.2 108.8 NDMSR8 31.7 32.2 31.0 32.2 28.8 36.3 31.7 33.0 NDPLR8 211.8 163.9 131.3 161.0 153.3 223.3 172.8 141.8 PDMSR8 100.7 127.3 135.7 136.0 106.0 112.3 116.7 157.0 PDNDBRR8 2.2 2.3 2.6 2.3 2.2 1.9 2.3 2.6 PDNDMSR8 3.2 4.0 4.4 4.3 3.7 3.1 3.8 4.8 Bobs/Knot 2.3 2.7 3.4 2.7 2.5 2.1 2.6 3.1 PDPPR8 480.0 423.9 420.2 419.2 388.2 449.5 429.0 437.5 PHTR8 1122.5 1118.6 1081.3 1152.5 1114.2 1185.5 1063.3 1114.9 SDPDR8 2.2 2.3 2.3 2.3 2.3 2.3 2.4 2.4 SNUM 1060.8 983.4 980.5 954.7 894.7 1023.3 1003.7 1043.4 TPBR8 379.3 296.6 284.5 283.2 282.2 337.2 312.3 280.5 SW1000 (grams) 152.2 154.1 163.6 151.6 159.8 148.4 153.7 147.3

[224] Soybean plants expressing pAt.Erl1::Gm.FT2a::T-GAPDH cassettes | pSl.MADS-RIN::miRNA-FT2a , flowered slightly earlier (DOFR1) and had a slightly increased number of pods per node (and a slightly increased number of pods per main stem) and a modest reduction in branching compared to DT control plants. Early field results with this construct also showed moderate early flowering, indicating that plants grown in the field will show similar moderate changes in generative traits.

Example 23 Promoter Modification of Gm.FT2a Ectopic ExpressionpAt.Erl1and miR156 sensor changes the flowering time.

[225] A transformation vector was constructed containing Gm.FT2a driven by the meristem-preferred vegetative stage promoter pAt.Erl1 (SEQ ID NO: 44) with the miR156 target site (SEQ ID NO: 106) and the GAPDH terminator region (T-GAPDH) and used to transform soybean by Agrobacterium -mediated transformation. The transgenic plants created by these events were tested in the field. Data were obtained for six plants for each DT event and control. Early field results with this construct demonstrated early flowering, indicating that plants grown in the greenhouse or in the field are likely to experience moderate changes in generative traits, including an increased number of pods per node.

Example 24 Modification of Gm.FT2a ectopic expression with a truncated pAt.Erecta promoter alters flowering time.

[226] A transformation vector containing Gm.FT2a driven by a shorter truncated pAt.Erecta promoter (SEQ ID NO: 48) with an Lhcb2 (T-Lhcb2) terminator region was constructed and used to transform soybean via Agrobacterium-mediated transformation. The transgenic plants created by these events were tested in the field. Data were obtained for six plants for each DT event and control. Early field results with this construct showed a slightly earlier onset of flowering, indicating that plants grown in the greenhouse or in the field are likely to show slight to moderate changes in generative traits, including a slightly increased number of pods per node. Thus, the most attenuated generative phenotypes and milder effects of early termination can be provided by this more minimal truncated pAt.Erecta promoter (compared to examples 3 and 17).

[227] After a detailed description of the present invention, the possibility of modifications, variations, and equivalent implementations will become apparent without departing from the spirit and scope of the present invention as described herein and in the appended claims. In addition, it should be understood that all examples in this description are given as non-limiting examples.

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SEQUENCE LIST

<110> MONSANTO TECHNOLOGY LLC

BROWER-TOLAND, Brent

Dai, Shunhong

GABBERT, Karen

GOLDSHMIDT, Alexander

HOWELL, Miya

MCDILL, Brad

OVADYA, Dan

SAVIDGE, Beth

SHARMA, Vijay

<120> COMPOSITIONS AND METHODS FOR FLOWER CHANGE

AND PLANT ARCHITECTURE TO IMPROVE YIELD POTENTIAL

<130> P34461WO00

<150> US 62/410355

<151> 2016-10-19

<150> US 62/411408

<151> 2016-10-21

<160> 110

<170> PatentIn version 3.5

<210> 1

<211> 531

<212> DNA

<213> Glycine max

<400> 1

atgcctagtg gaagtaggga tcctctcgtt gttgggggag taattgggga tgtattggat 60

ccttttgaat attctattcc tatgaggggtt acctacaata acagagatgt cagcaatgga 120

tgtgaattca aaccctcaca agttgtcaac caaccaaggg taaatatcgg tggtgatgac 180

ctcaggaact tctatacttt gattgcggtt gatcccgatg cacctagccc aagtgacccc 240

aatttgagag aatacctcca ttggttggtg actgatatcc cagcaacaac aggggctagt 300

ttcggccatg aggttgtaac atatgaaagt ccaagaccaa tgatggggat tcatcgtttg 360

gtgtttgtgt tatttcgtca actgggtagg gagaccgtgt atgcaccagg atggcgccag 420

aatttcaaca ctaaagaatt tgctgaactt tacaaccttg gattgccagt tgctgctgtc 480

tatttcaaca ttcagaggga atctggttct ggtggaagga ggttatacta a 531

<210> 2

<211> 176

<212> Protein

<213> Glycine max

<400> 2

Met Pro Ser Gly Ser Arg Asp Pro Leu Val Val Gly Gly Val Ile Gly

1 5 10 15

Asp Val Leu Asp Pro Phe Glu Tyr Ser Ile Pro Met Arg Val Thr Tyr

20 25 30

Asn Asn Arg Asp Val Ser Asn Gly Cys Glu Phe Lys Pro Ser Gln Val

35 40 45

Val Asn Gln Pro Arg Val Asn Ile Gly Gly Asp Asp Leu Arg Asn Phe

50 55 60

Tyr Thr Leu Ile Ala Val Asp Pro Asp Ala Pro Ser Pro Ser Asp Pro

65 70 75 80

Asn Leu Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Ala Thr

85 90 95

Thr Gly Ala Ser Phe Gly His Glu Val Val Thr Tyr Glu Ser Pro Arg

100 105 110

Pro Met Met Gly Ile His Arg Leu Val Phe Val Leu Phe Arg Gln Leu

115 120 125

Gly Arg Glu Thr Val Tyr Ala Pro Gly Trp Arg Gln Asn Phe Asn Thr

130 135 140

Lys Glu Phe Ala Glu Leu Tyr Asn Leu Gly Leu Pro Val Ala Ala Val

145 150 155 160

Tyr Phe Asn Ile Gln Arg Glu Ser Gly Ser Gly Gly Arg Arg Leu Tyr

165 170 175

<210> 3

<211> 519

<212> DNA

<213> Glycine max

<400> 3

atggcacggg agaaccctct tgttattggt ggtgtgattg gggatgttct caaccctttt 60

acaagctccg tttctttgac tgtttcaatc aataataggg cgattagcaa tggcttggaa 120

ctcaggccct ctcaagttgt taatcgccct agggttactg ttggtggtga agacctaagg 180

accttctaca ctctggttat ggtggatgca gatgcaccta gccctagcaa ccctgtcttg 240

agggaatacc ttcactggat ggtgacagat attccagcta ccacaaatgc aagctttggg 300

agagaggttg tgttttatga gagcccgaac ccttcagtag ggattcatcg aatcgtgttc 360

gtattgttcc agcaattggg cagagacact gtcatcaccc cagaatggcg ccataatttc 420

aattccagaa actttgctga aattaataac cttgcacctg ttgcagcagc ttatgccaac 480

tgccaaagag agcgtggttg cggtggaagg agatattaa 519

<210> 4

<211> 172

<212> Protein

<213> Glycine max

<400> 4

Met Ala Arg Glu Asn Pro Leu Val Ile Gly Gly Val Ile Gly Asp Val

1 5 10 15

Leu Asn Pro Phe Thr Ser Ser Val Ser Leu Thr Val Ser Ile Asn Asn

20 25 30

Arg Ala Ile Ser Asn Gly Leu Glu Leu Arg Pro Ser Gln Val Val Asn

35 40 45

Arg Pro Arg Val Thr Val Gly Gly Glu Asp Leu Arg Thr Phe Tyr Thr

50 55 60

Leu Val Met Val Asp Ala Asp Ala Pro Ser Pro Ser Asn Pro Val Leu

65 70 75 80

Arg Glu Tyr Leu His Trp Met Val Thr Asp Ile Pro Ala Thr Thr Asn

85 90 95

Ala Ser Phe Gly Arg Glu Val Val Phe Tyr Glu Ser Pro Asn Pro Ser

100 105 110

Val Gly Ile His Arg Ile Val Phe Val Leu Phe Gln Gln Leu Gly Arg

115 120 125

Asp Thr Val Ile Thr Pro Glu Trp Arg His Asn Phe Asn Ser Arg Asn

130 135 140

Phe Ala Glu Ile Asn Asn Leu Ala Pro Val Ala Ala Ala Tyr Ala Asn

145 150 155 160

Cys Gln Arg Glu Arg Gly Cys Gly Gly Arg Arg Tyr

165 170

<210> 5

<211> 531

<212> DNA

<213> Glycine max

<400> 5

atgcctcgtg gaagtaggga ccctctagtt gttgggcgtg tgattgggga tgtattggac 60

ccttttgaat gttctattcc tatgagggtc acctacaata acaaagatgt cagcaatgga 120

tgtgaattca aaccctcaca agttgtcaac caaccaagaa taaatatcgg tggtgatgat 180

ttcaggaact tctacacttt gatcgcggtt gatcctgatg cacctagccc aagtgatccc 240

aatttcagag aatacctcca ttggttagta actgacattc cagcaacaac ggggcctact 300

ttcggtcatg aggttgtaac atatgaaaat ccacgaccca tgatggggat ccatcgtata 360

gtctttgtgt tatttcgtca acagggtaga gagacagtgt atgcaccagg atggcgccaa 420

aatttcatta ctagagaatt tgctgaactt tacaatcttg gattgccagt tgctgctgtc 480

a 531

<210> 6

<211> 176

<212> Protein

<213> Glycine max

<400> 6

Met Pro Arg Gly Ser Arg Asp Pro Leu Val Val Gly Arg Val Ile Gly

1 5 10 15

Asp Val Leu Asp Pro Phe Glu Cys Ser Ile Pro Met Arg Val Thr Tyr

20 25 30

Asn Asn Lys Asp Val Ser Asn Gly Cys Glu Phe Lys Pro Ser Gln Val

35 40 45

Val Asn Gln Pro Arg Ile Asn Ile Gly Gly Asp Asp Phe Arg Asn Phe

50 55 60

Tyr Thr Leu Ile Ala Val Asp Pro Asp Ala Pro Ser Pro Ser Asp Pro

65 70 75 80

Asn Phe Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Ala Thr

85 90 95

Thr Gly Pro Thr Phe Gly His Glu Val Val Thr Tyr Glu Asn Pro Arg

100 105 110

Pro Met Met Gly Ile His Arg Ile Val Phe Val Leu Phe Arg Gln Gln

115 120 125

Gly Arg Glu Thr Val Tyr Ala Pro Gly Trp Arg Gln Asn Phe Ile Thr

130 135 140

Arg Glu Phe Ala Glu Leu Tyr Asn Leu Gly Leu Pro Val Ala Ala Val

145 150 155 160

Tyr Phe Asn Ile Gln Arg Glu Ser Gly Cys Gly Gly Arg Arg Leu Cys

165 170 175

<210> 7

<211> 528

<212> DNA

<213> Zea mays

<400> 7

atgtcagcaa ccgatcattt ggttatggct cgtgtcatac aggatgtatt ggatcccttt 60

acaccaacca ttcactaag aataacgtac aacaataggc tacttctgcc aagtgctgag 120

ctaaagccat ccgcggttgt aagtaaacca cgagtcgata tcggtggcag tgacatgagg 180

gctttctaca ccctggtact gattgacccg gatgccccaa gtccaagcca tccatcacta 240

agggagtact tgcactggat ggtgacagat attccagaaa caactagtgt caactttggc 300

caagagctaa tattttatga gaggccggac ccaagatctg gcatccacag gctggtattt 360

gtgctgttcc gtcaacttgg cagggggaca gtttttgcac cagaaatgcg ccacaacttc 420

aactgcagaa gctttgcacg gcaatatcac ctcagcattg ccaccgctac acatttcaac 480

tgtcaaaggg aaggtggatc cggcggaaga aggtttaggg aaagtag 528

<210> 8

<211> 175

<212> Protein

<213> Zea mays

<400> 8

Met Ser Ala Thr Asp His Leu Val Met Ala Arg Val Ile Gln Asp Val

1 5 10 15

Leu Asp Pro Phe Thr Pro Thr Ile Pro Leu Arg Ile Thr Tyr Asn Asn

20 25 30

Arg Leu Leu Leu Pro Ser Ala Glu Leu Lys Pro Ser Ala Val Val Ser

35 40 45

Lys Pro Arg Val Asp Ile Gly Ser Asp Met Arg Ala Phe Tyr Thr

50 55 60

Leu Val Leu Ile Asp Pro Asp Ala Pro Ser Pro Ser His Pro Ser Leu

65 70 75 80

Arg Glu Tyr Leu His Trp Met Val Thr Asp Ile Pro Glu Thr Thr Ser

85 90 95

Val Asn Phe Gly Gln Glu Leu Ile Phe Tyr Glu Arg Pro Asp Pro Arg

100 105 110

Ser Gly Ile His Arg Leu Val Phe Val Leu Phe Arg Gln Leu Gly Arg

115 120 125

Gly Thr Val Phe Ala Pro Glu Met Arg His Asn Phe Asn Cys Arg Ser

130 135 140

Phe Ala Arg Gln Tyr His Leu Ser Ile Ala Thr Ala Thr His Phe Asn

145 150 155 160

Cys Gln Arg Glu Gly Gly Ser Gly Gly Arg Arg Phe Arg Glu Glu

165 170 175

<210> 9

<211> 525

<212> DNA

<213> Nicotiana tabacum

<400> 9

atgccaagaa tagatccttt gatagttggt cgtgtggtag gagatgtttt agatccattc 60

acaaggtctg ttgatcttag agtggtttac aataataggg aagtcaacaa tgcatgtggc 120

ttgaaacctt ctcaaattgt tacgcaacct agggttcaaa ttggagggga tgatcttcgc 180

aacttttaca ctctggttat ggtggatcct gatgctccaa gcccaagcaa ccctaacctg 240

agggagtatc tacactggct ggtcacagat atcccagcaa ctacagatac aagctttgga 300

aatgaagtta tatgctacga gaatccacaa ccatcattgg gaattcatcg ctttgttttc 360

gtgttgtttc gacaattggg tcgcgaaact gtgtatgcac caggttggcg tcagaatttc 420

agcacaagag actttgcaga agtttacaat cttggtttgc ccgtttctgc tgtttacttc 480

aattgccata gggagagtgg tactggtggc cgccgcgcat attaa 525

<210> 10

<211> 174

<212> Protein

<213> Nicotiana tabacum

<400> 10

Met Pro Arg Ile Asp Pro Leu Ile Val Gly Arg Val Val Gly Asp Val

1 5 10 15

Leu Asp Pro Phe Thr Arg Ser Val Asp Leu Arg Val Val Tyr Asn Asn

20 25 30

Arg Glu Val Asn Asn Ala Cys Gly Leu Lys Pro Ser Gln Ile Val Thr

35 40 45

Gln Pro Arg Val Gln Ile Gly Gly Asp Asp Leu Arg Asn Phe Tyr Thr

50 55 60

Leu Val Met Val Asp Pro Asp Ala Pro Ser Pro Ser Asn Pro Asn Leu

65 70 75 80

Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Ala Thr Thr Asp

85 90 95

Thr Ser Phe Gly Asn Glu Val Ile Cys Tyr Glu Asn Pro Gln Pro Ser

100 105 110

Leu Gly Ile His Arg Phe Val Phe Val Leu Phe Arg Gln Leu Gly Arg

115 120 125

Glu Thr Val Tyr Ala Pro Gly Trp Arg Gln Asn Phe Ser Thr Arg Asp

130 135 140

Phe Ala Glu Val Tyr Asn Leu Gly Leu Pro Val Ser Ala Val Tyr Phe

145 150 155 160

Asn Cys His Arg Glu Ser Gly Thr Gly Gly Arg Arg Ala Tyr

165 170

<210> 11

<211> 534

<212> DNA

<213> Solanum lycopersicum

<400> 11

atgcctagag aacgtgatcc tcttgttgtt ggtcgtgtgg taggggatgt attggaccct 60

ttcacaagaa ctattggcct aagagttata tatagagata gagaagttaa taatggatgc 120

gagcttaggc cttcccaagt tattaaccag ccaagggttg aagttggagg agatgaccta 180

cgtacctttt tcactttggt tatggtggac cctgatgctc caagtccgag tgatccaaat 240

ctgagagaat accttcactg gttggtcacc gatattccag ctaccacagg ttcaagtttt 300

360

tttgtattat tcagacaatt aggtcggcaa acagtgtatg ctccaggatg gcgtcagaat 420

ttcaacacaa gagattttgc agaactttat aatcttggtt tacctgttgc tgctgtctat 480

tttaattgtc aaagagagag tggcagtggt ggacgtagaa gatctgctga ttga 534

<210> 12

<211> 177

<212> Protein

<213> Solanum lycopersicum

<400> 12

Met Pro Arg Glu Arg Asp Pro Leu Val Val Gly Arg Val Val Gly Asp

1 5 10 15

Val Leu Asp Pro Phe Thr Arg Thr Ile Gly Leu Arg Val Ile Tyr Arg

20 25 30

Asp Arg Glu Val Asn Asn Gly Cys Glu Leu Arg Pro Ser Gln Val Ile

35 40 45

Asn Gln Pro Arg Val Glu Val Gly Gly Asp Asp Leu Arg Thr Phe Phe

50 55 60

Thr Leu Val Met Val Asp Pro Asp Ala Pro Ser Pro Ser Asp Pro Asn

65 70 75 80

Leu Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Ala Thr Thr

85 90 95

Gly Ser Ser Phe Gly Gln Glu Ile Val Ser Tyr Glu Ser Pro Arg Pro

100 105 110

Ser Met Gly Ile His Arg Phe Val Phe Val Leu Phe Arg Gln Leu Gly

115 120 125

Arg Gln Thr Val Tyr Ala Pro Gly Trp Arg Gln Asn Phe Asn Thr Arg

130 135 140

Asp Phe Ala Glu Leu Tyr Asn Leu Gly Leu Pro Val Ala Ala Val Tyr

145 150 155 160

Phe Asn Cys Gln Arg Glu Ser Gly Ser Gly Gly Arg Arg Arg Ser Ala

165 170 175

asp

<210> 13

<211> 528

<212> DNA

<213> Arabidopsis thaliana

<400> 13

atgtctataa atataagaga ccctcttata gtaagcagag ttgttggaga cgttcttgat 60

ccgtttaata gatcaatcac tctaaaggtt acttatggcc aaagagaggt gactaatggc 120

ttggatctaa ggccttctca ggttcaaaac aagccaagag ttgagattgg tggagaagac 180

ctcaggaact tctatacttt ggttatggtg gatccagatg ttccaagtcc tagcaaccct 240

cacctccgag aatatctcca ttggttggtg actgatatcc ctgctacaac tggaacaacc 300

360

gtgtttatat tgtttcgaca gcttggcagg caaacagtgt atgcaccagg gtggcgccag 420

aacttcaaca ctcgcgagtt tgctgagatc tacaatctcg gccttcccgt ggccgcagtt 480

ttctacaatt gtcagaggga gagtggctgc ggaggaagaa gactttag 528

<210> 14

<211> 175

<212> Protein

<213> Arabidopsis thaliana

<400> 14

Met Ser Ile Asn Ile Arg Asp Pro Leu Ile Val Ser Arg Val Val Gly

1 5 10 15

Asp Val Leu Asp Pro Phe Asn Arg Ser Ile Thr Leu Lys Val Thr Tyr

20 25 30

Gly Gln Arg Glu Val Thr Asn Gly Leu Asp Leu Arg Pro Ser Gln Val

35 40 45

Gln Asn Lys Pro Arg Val Glu Ile Gly Gly Glu Asp Leu Arg Asn Phe

50 55 60

Tyr Thr Leu Val Met Val Asp Pro Asp Val Pro Ser Pro Ser Asn Pro

65 70 75 80

His Leu Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Ala Thr

85 90 95

Thr Gly Thr Thr Phe Gly Asn Glu Ile Val Cys Tyr Glu Asn Pro Ser

100 105 110

Pro Thr Ala Gly Ile His Arg Val Val Phe Ile Leu Phe Arg Gln Leu

115 120 125

Gly Arg Gln Thr Val Tyr Ala Pro Gly Trp Arg Gln Asn Phe Asn Thr

130 135 140

Arg Glu Phe Ala Glu Ile Tyr Asn Leu Gly Leu Pro Val Ala Ala Val

145 150 155 160

Phe Tyr Asn Cys Gln Arg Glu Ser Gly Cys Gly Gly Arg Arg Leu

165 170 175

<210> 15

<211> 528

<212> DNA

<213> Arabidopsis thaliana

<400> 15

atgtctttaa gtcgtagaga tcctcttgtg gtcggcagtg ttgttggaga tgttcttgat 60

cctttcacga ggttggtctc tcttaaggtc acttatggcc atagagaggt tactaatggc 120

ttggatctaa ggccttctca agttctgaac aaaccaatag tggagattgg aggagacgac 180

ttcagaaatt tctacacctt ggttatggtg gatccagatg tgccgagtcc aagcaaccct 240

caccaacgag aatatctcca ctggttggtg actgatatac ctgccaccac tggaaatgcc 300

tttggcaatg aggtggtgtg ctacgagagt ccacgtcccc cctcgggaat tcatcgtatt 360

gtgttggtat tgttccggca actcggaaga caaacggttt atgcaccggg gtggcgccaa 420

cagttcaaca ctcgtgagtt tgctgagatc tacaatcttg gtcttcctgt ggctgcctct 480

tacttcaact gccagaggga gaatggctgt gggggaagaa gaacgtag 528

<210> 16

<211> 175

<212> Protein

<213> Arabidopsis thaliana

<400> 16

Met Ser Leu Ser Arg Arg Asp Pro Leu Val Val Gly Ser Val Val Gly

1 5 10 15

Asp Val Leu Asp Pro Phe Thr Arg Leu Val Ser Leu Lys Val Thr Tyr

20 25 30

Gly His Arg Glu Val Thr Asn Gly Leu Asp Leu Arg Pro Ser Gln Val

35 40 45

Leu Asn Lys Pro Ile Val Glu Ile Gly Gly Asp Asp Phe Arg Asn Phe

50 55 60

Tyr Thr Leu Val Met Val Asp Pro Asp Val Pro Ser Pro Ser Asn Pro

65 70 75 80

His Gln Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Ala Thr

85 90 95

Thr Gly Asn Ala Phe Gly Asn Glu Val Val Cys Tyr Glu Ser Pro Arg

100 105 110

Pro Pro Ser Gly Ile His Arg Ile Val Leu Val Leu Phe Arg Gln Leu

115 120 125

Gly Arg Gln Thr Val Tyr Ala Pro Gly Trp Arg Gln Gln Phe Asn Thr

130 135 140

Arg Glu Phe Ala Glu Ile Tyr Asn Leu Gly Leu Pro Val Ala Ala Ser

145 150 155 160

Tyr Phe Asn Cys Gln Arg Glu Asn Gly Cys Gly Gly Arg Arg Thr

165 170 175

<210> 17

<211> 540

<212> DNA

<213> Oryza sativa

<400> 17

atggccggaa gtggcaggga cagggaccct cttgtggttg gtagggttgt gggtgatgtg 60

ctggacgcgt tcgtccggag caccaacctc aaggtcacct atggctccaa gaccgtgtcc 120

aatggctgcg agctcaagcc gtccatggtc acccaccagc ctagggtcga ggtcggcggc 180

aatgacatga ggacattcta cacccttgtg atggtagacc cagatgcacc aagcccaagt 240

gaccctaacc ttagggagta tctacattgg ttggtcactg atattcctgg tactactgca 300

gcgtcatttg ggcaagaggt gatgtgctac gagagcccaa ggccaaccat ggggatccac 360

cggctggtgt tcgtgctgtt ccagcagctg gggcgtcaga cagtgtacgc gcccgggtgg 420

cgtcagaact tcaacaccaa ggacttcgcc gagctctaca acctcggctc gccggtcgcc 480

gccgtctact tcaactgcca gcgcgaggca ggctccggcg gcaggagggt ctacccctag 540

<210> 18

<211> 179

<212> Protein

<213> Oryza sativa

<400> 18

Met Ala Gly Ser Gly Arg Asp Arg Asp Pro Leu Val Val Gly Arg Val

1 5 10 15

Val Gly Asp Val Leu Asp Ala Phe Val Arg Ser Thr Asn Leu Lys Val

20 25 30

Thr Tyr Gly Ser Lys Thr Val Ser Asn Gly Cys Glu Leu Lys Pro Ser

35 40 45

Met Val Thr His Gln Pro Arg Val Glu Val Gly Gly Asn Asp Met Arg

50 55 60

Thr Phe Tyr Thr Leu Val Met Val Asp Pro Asp Ala Pro Ser Pro Ser

65 70 75 80

Asp Pro Asn Leu Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro

85 90 95

Gly Thr Thr Ala Ala Ser Phe Gly Gln Glu Val Met Cys Tyr Glu Ser

100 105 110

Pro Arg Pro Thr Met Gly Ile His Arg Leu Val Phe Val Leu Phe Gln

115 120 125

Gln Leu Gly Arg Gln Thr Val Tyr Ala Pro Gly Trp Arg Gln Asn Phe

130 135 140

Asn Thr Lys Asp Phe Ala Glu Leu Tyr Asn Leu Gly Ser Pro Val Ala

145 150 155 160

Ala Val Tyr Phe Asn Cys Gln Arg Glu Ala Gly Ser Gly Gly Arg Arg

165 170 175

Val Tyr Pro

<210> 19

<211> 525

<212> DNA

<213> Populus trichocarpa

<400> 19

atgtcaaggg acagagatcc tctgagcgtt ggccgtgtta taggggacgt gctggacccc 60

ttcacaaagt ctatctccct cagggtcact tacagctcca gagaggtcaa caatggttgc 120

gagctcaagc ccctctcaggt tgccaaccag cctagggttg atattggcgg ggaagatcta 180

aggaccttct acactctggt tatggtggac cctgatgcac ccagcccaag tgaccccagc 240

ctaagagaat atttgcattg gttggtgact gatattccag caacaactgg ggcaagcttt 300

360

ttcgtcttgt ttcggcaact gggcaggcaa actgtgtatg cccctggggtg gcgccagaac 420

ttcaacacca gagactttgc tgaggtctac aatcttggat cgccagtggc tgctgtttat 480

ttcaactgcc aggggagag tggctctggt ggtaggaggc gataa 525

<210> 20

<211> 174

<212> Protein

<213> Populus trichocarpa

<400> 20

Met Ser Arg Asp Arg Asp Pro Leu Ser Val Gly Arg Val Ile Gly Asp

1 5 10 15

Val Leu Asp Pro Phe Thr Lys Ser Ile Ser Leu Arg Val Thr Tyr Ser

20 25 30

Ser Arg Glu Val Asn Asn Gly Cys Glu Leu Lys Pro Ser Gln Val Ala

35 40 45

Asn Gln Pro Arg Val Asp Ile Gly Gly Glu Asp Leu Arg Thr Phe Tyr

50 55 60

Thr Leu Val Met Val Asp Pro Asp Ala Pro Ser Pro Ser Asp Pro Ser

65 70 75 80

Leu Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Ala Thr Thr

85 90 95

Gly Ala Ser Phe Gly His Glu Thr Val Cys Tyr Glu Ser Pro Arg Pro

100 105 110

Thr Met Gly Ile His Arg Phe Val Phe Val Leu Phe Arg Gln Leu Gly

115 120 125

Arg Gln Thr Val Tyr Ala Pro Gly Trp Arg Gln Asn Phe Asn Thr Arg

130 135 140

Asp Phe Ala Glu Val Tyr Asn Leu Gly Ser Pro Val Ala Ala Val Tyr

145 150 155 160

Phe Asn Cys Gln Arg Glu Ser Gly Ser Gly Gly Arg Arg Arg

165 170

<210> 21

<211> 519

<212> DNA

<213> Glycine max

<400> 21

atggcacggg agaaccctct tgttattggg ggtgtgattg gggatgttct caatcctttt 60

acaatctccg tttcttttac tatttcaatc aataataggg cgattagcaa tggcttggaa 120

ctgaggccct ctcaagttgt taatcgccct agagtcactg ttggtggtga agacctaagg 180

accttctaca cactggttat ggtggatgca gatgcaccta gccctagcaa ccctgtcttg 240

agggaatacc ttcactggat ggtgacagat attccagcta ccacaaatgc aagctttggg 300

agagaggttg tgttttatga gagcccgaac ccttcagcag ggattcatcg acttgtgttc 360

atattattcc agcaactggg cagagacact gtcatcaccc cagaatggcg ccataatttc 420

aattccagaa actttgctga aattaataac cttgcacctg ttgcagcagc ttatgccaac 480

tgccaaagag agcgtggttg cggtggaagg agatattaa 519

<210> 22

<211> 172

<212> Protein

<213> Glycine max

<400> 22

Met Ala Arg Glu Asn Pro Leu Val Ile Gly Gly Val Ile Gly Asp Val

1 5 10 15

Leu Asn Pro Phe Thr Ile Ser Val Ser Phe Thr Ile Ser Ile Asn Asn

20 25 30

Arg Ala Ile Ser Asn Gly Leu Glu Leu Arg Pro Ser Gln Val Val Asn

35 40 45

Arg Pro Arg Val Thr Val Gly Gly Glu Asp Leu Arg Thr Phe Tyr Thr

50 55 60

Leu Val Met Val Asp Ala Asp Ala Pro Ser Pro Ser Asn Pro Val Leu

65 70 75 80

Arg Glu Tyr Leu His Trp Met Val Thr Asp Ile Pro Ala Thr Thr Asn

85 90 95

Ala Ser Phe Gly Arg Glu Val Val Phe Tyr Glu Ser Pro Asn Pro Ser

100 105 110

Ala Gly Ile His Arg Leu Val Phe Ile Leu Phe Gln Gln Leu Gly Arg

115 120 125

Asp Thr Val Ile Thr Pro Glu Trp Arg His Asn Phe Asn Ser Arg Asn

130 135 140

Phe Ala Glu Ile Asn Asn Leu Ala Pro Val Ala Ala Ala Tyr Ala Asn

145 150 155 160

Cys Gln Arg Glu Arg Gly Cys Gly Gly Arg Arg Tyr

165 170

<210> 23

<211> 525

<212> DNA

<213> Gossypium hirsutum

<400> 23

atgcctagag acagagatcc tttggttgtt ggtagggtta taggagatgt gttggaccct 60

tttacaaggt ctatttcact tagggtcact tatgctacta gggatgttag caatggtgtt 120

gagcttaagc catctcaagt tgttaaccaa ccaagggttg atattggtgg ggatgatctg 180

aggaccttct acaccttggt tatggtggat cctgatgctc caagtccaag tgacccaaac 240

ctcagggaat acttgcactg gttggttact gatattccag ccacaactgg tgcaagcttt 300

ggtcaagagg tggtctgcta tgagagccca cgaccaacgg tcggtatcca tcgttttgtg 360

ttcgtgctgt tccggcaact tggaaggcaa acggtgtacg ctccagggtg gcgccaaaac 420

ttcaacacta gggactttgc tgagctttac aacctcgggt tgccggtggc tgctgtttac 480

tttaactgcc agaggggagag tggatccggt ggccgtagga catga 525

<210> 24

<211> 174

<212> Protein

<213> Gossypium hirsutum

<400> 24

Met Pro Arg Asp Arg Asp Pro Leu Val Val Gly Arg Val Ile Gly Asp

1 5 10 15

Val Leu Asp Pro Phe Thr Arg Ser Ile Ser Leu Arg Val Thr Tyr Ala

20 25 30

Thr Arg Asp Val Ser Asn Gly Val Glu Leu Lys Pro Ser Gln Val Val

35 40 45

Asn Gln Pro Arg Val Asp Ile Gly Gly Asp Asp Leu Arg Thr Phe Tyr

50 55 60

Thr Leu Val Met Val Asp Pro Asp Ala Pro Ser Pro Ser Asp Pro Asn

65 70 75 80

Leu Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Ala Thr Thr

85 90 95

Gly Ala Ser Phe Gly Gln Glu Val Val Cys Tyr Glu Ser Pro Arg Pro

100 105 110

Thr Val Gly Ile His Arg Phe Val Phe Val Leu Phe Arg Gln Leu Gly

115 120 125

Arg Gln Thr Val Tyr Ala Pro Gly Trp Arg Gln Asn Phe Asn Thr Arg

130 135 140

Asp Phe Ala Glu Leu Tyr Asn Leu Gly Leu Pro Val Ala Ala Val Tyr

145 150 155 160

Phe Asn Cys Gln Arg Glu Ser Gly Ser Gly Gly Arg Arg Thr

165 170

<210> 25

<211> 528

<212> DNA

<213> Brassica napus

<400> 25

atgtctttaa gtaatagaga tcctcttgtg gtagggagag ttgtaggaga cgttcttgaa 60

tgtttcacaa gatcaatcga tctaagggtt acttatggcc aaagagaggt gacaaatggg 120

ttggatctaa ggccttctca agttctcaac aagccaagag ttgagattgg tggagaagac 180

ctaaggaact tctatacttt ggttatggtg gatccagatg ttccaagtcc tagcaatcct 240

cacctccgag aatatcttca ctggttggtg actgatatcc cagcgacaac tggaacaaac 300

tttggcaatg agattgtgtc ttacgagagt ccaaggccca actcgggtat tcatcgtatc 360

gtgctcgtat tgttccgaca gctcggtagg caaacagtgt atgaaccagg atggcgccaa 420

caattcaaca ctcgtgagtt tgcttcccta tacaatctcg gccttcccgt ggctgcggtt 480

ttctacaatt gtcagaggga gagtggctgc ggaggacgaa gaagttag 528

<210> 26

<211> 175

<212> Protein

<213> Brassica napus

<400> 26

Met Ser Leu Ser Asn Arg Asp Pro Leu Val Val Gly Arg Val Val Gly

1 5 10 15

Asp Val Leu Glu Cys Phe Thr Arg Ser Ile Asp Leu Arg Val Thr Tyr

20 25 30

Gly Gln Arg Glu Val Thr Asn Gly Leu Asp Leu Arg Pro Ser Gln Val

35 40 45

Leu Asn Lys Pro Arg Val Glu Ile Gly Gly Glu Asp Leu Arg Asn Phe

50 55 60

Tyr Thr Leu Val Met Val Asp Pro Asp Val Pro Ser Pro Ser Asn Pro

65 70 75 80

His Leu Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Ala Thr

85 90 95

Thr Gly Thr Asn Phe Gly Asn Glu Ile Val Ser Tyr Glu Ser Pro Arg

100 105 110

Pro Asn Ser Gly Ile His Arg Ile Val Leu Val Leu Phe Arg Gln Leu

115 120 125

Gly Arg Gln Thr Val Tyr Glu Pro Gly Trp Arg Gln Gln Phe Asn Thr

130 135 140

Arg Glu Phe Ala Ser Leu Tyr Asn Leu Gly Leu Pro Val Ala Ala Val

145 150 155 160

Phe Tyr Asn Cys Gln Arg Glu Ser Gly Cys Gly Gly Arg Arg Ser

165 170 175

<210> 27

<211> 543

<212> DNA

<213> Triticum aestivum

<400> 27

atgtcggcag cggatccatt ggttgtggct catgttttac aagatgtgct tgatccattt 60

acatcaactg ttccgctcag gatagcctac aacaataggc tagttctggc aggtgctgag 120

ctaagaccat ctgcaattgt aagcaagcca cgagttgata tcggtggcag tgacatgaga 180

gtcctctata ccctgatatt ggtggatcca gacgccccaa gcccaagtca cccatcacta 240

agggagtact tgcactggat ggtgtccgac atccctggaa caactagtgg cagcttcggc 300

caagagcttg tagtttatga aagaccagaa cccagatctg gtattcaccg gatggtattt 360

gtgctgttcc agcaactagg caggggaaca gtttttgcac cagatgtgcg acacaatttc 420

agctgcagaa actttgcacg gcagtaccac ctcaacattg tggctgcctc atatttcaac 480

tgtcaaaggg aaggtggatc tggcggaaga aggtttaggc cagaaagttc tcaaggggag 540

tag 543

<210> 28

<211> 180

<212> Protein

<213> Triticum aestivum

<400> 28

Met Ser Ala Ala Asp Pro Leu Val Val Ala His Val Leu Gln Asp Val

1 5 10 15

Leu Asp Pro Phe Thr Ser Thr Val Pro Leu Arg Ile Ala Tyr Asn Asn

20 25 30

Arg Leu Val Leu Ala Gly Ala Glu Leu Arg Pro Ser Ala Ile Val Ser

35 40 45

Lys Pro Arg Val Asp Ile Gly Gly Ser Asp Met Arg Val Leu Tyr Thr

50 55 60

Leu Ile Leu Val Asp Pro Asp Ala Pro Ser Pro Ser His Pro Ser Leu

65 70 75 80

Arg Glu Tyr Leu His Trp Met Val Ser Asp Ile Pro Gly Thr Thr Ser

85 90 95

Gly Ser Phe Gly Gln Glu Leu Val Val Tyr Glu Arg Pro Glu Pro Arg

100 105 110

Ser Gly Ile His Arg Met Val Phe Val Leu Phe Gln Gln Leu Gly Arg

115 120 125

Gly Thr Val Phe Ala Pro Asp Val Arg His Asn Phe Ser Cys Arg Asn

130 135 140

Phe Ala Arg Gln Tyr His Leu Asn Ile Val Ala Ala Ser Tyr Phe Asn

145 150 155 160

Cys Gln Arg Glu Gly Gly Ser Gly Gly Arg Arg Phe Arg Pro Glu Ser

165 170 175

Ser Gln Gly Glu

180

<210> 29

<211> 531

<212> DNA

<213> Pisum sativum

<400> 29

atggcaggta gtagcaggaa tcctctcgct gttggtcgtg taattggtga tgtgatagac 60

ccctttgaaa attcggttcc tctccgagtc acctatggta gtagagatgt gaataatggt 120

tgtgagctta aaccctctca cgttggaaat caacccagag tgaatgttgg tggaaacgat 180

ctcaggaaca tttatactct agttcttgtg gacccagatt cacctagccc aagcaaccct 240

acttttaggg agtaccttca ttggttggtg actgatattc cagctactac tgaggtcagt 300

ttcggtaacg aaattgtgag ctatgaaagg ccacgaccca cctcagggat ccatcgtttc 360

gtgtttatac tattccgtca gcagtgtaga caaagggttt acgctccagg atggcgacag 420

aatttcaata caagagaatt tgctgaactt tacaatcttg gatcaccagt tgctgctgtt 480

ttcttcaact gtcaaaggga gagtggctct ggtggaagaa catttagata a 531

<210> 30

<211> 176

<212> Protein

<213> Pisum sativum

<400> 30

Met Ala Gly Ser Ser Arg Asn Pro Leu Ala Val Gly Arg Val Ile Gly

1 5 10 15

Asp Val Ile Asp Pro Phe Glu Asn Ser Val Pro Leu Arg Val Thr Tyr

20 25 30

Gly Ser Arg Asp Val Asn Asn Gly Cys Glu Leu Lys Pro Ser His Val

35 40 45

Gly Asn Gln Pro Arg Val Asn Val Gly Gly Asn Asp Leu Arg Asn Ile

50 55 60

Tyr Thr Leu Val Leu Val Asp Pro Asp Ser Pro Ser Pro Ser Asn Pro

65 70 75 80

Thr Phe Arg Glu Tyr Leu His Trp Leu Val Thr Asp Ile Pro Ala Thr

85 90 95

Thr Glu Val Ser Phe Gly Asn Glu Ile Val Ser Tyr Glu Arg Pro Arg

100 105 110

Pro Thr Ser Gly Ile His Arg Phe Val Phe Ile Leu Phe Arg Gln Gln

115 120 125

Cys Arg Gln Arg Val Tyr Ala Pro Gly Trp Arg Gln Asn Phe Asn Thr

130 135 140

Arg Glu Phe Ala Glu Leu Tyr Asn Leu Gly Ser Pro Val Ala Ala Val

145 150 155 160

Phe Phe Asn Cys Gln Arg Glu Ser Gly Ser Gly Gly Arg Thr Phe Arg

165 170 175

<210> 31

<211> 901

<212> DNA

<213> Arabidopsis thaliana

<400> 31

aaaccgaccg gagccaacca aaccggttaa catcctaaaa ccaatcatat tttattaagt 60

tttgtgttga tgctaaacca aaaatcattg gcatgcatat ttctaaattt agtaataaac 120

aaaaacactt agaaatcaca cgttcactat actaaaaaac gttgacaaaa acacaacaac 180

tatactaata attaaagaag agaaaactga accaaacttt ttgtaaactc ctgaatttaa 240

attagtaatt gaagtaagaa gatgaagaag aacatgttaa gcaaacaaaa aaattacact 300

aaaatcatat aaaaatacat aattacaaaa gtacccataa gatggattta ttgatatggg 360

tcatctgtga aacaagccac agagagacaa agactcgtaa gtattgggca acgaaagcga 420

cctcctttat tcaccactgc cattaacatg ttcttcttct ccttcttctt ctacatttta 480

tgaccgtttt acccttcaag agagagaaac aaaatcactc cctctcactc actctatctc 540

tctcttctgc aaagcttcag aactctggca gagagataaa agatgatggg gtttttaact 600

ttatcctccc caaataattc ttcttccctt catctctctc tcttacacaa caggtcccta 660

catttgtaca atctcctctc tttaaagact ctctctcttt ctctctccat ctctatctta 720

ctctgtattt ctgtcgtctg agcactcaat gaaaccactg taaatttccg ccagaatttg 780

atgtgatgga acgataaaaa tcattttttc tcggttaaag taaaaaaaca aaaacaaatt 840

tctgtagaaa tcataataaa agaaagaaaa aaaatctaat gtcggtacat aatacggttc 900

t 901

<210> 32

<211> 507

<212> DNA

<213> Arabidopsis thaliana

<400> 32

aaaccgaccg gagccaacca aaccggttaa catcctaaaa ccaatcatat tttattaagt 60

tttgtgttga tgctaaacca aaaatcattg gcatgcatat ttctaaattt agtaataaac 120

aaaaacactt agaaatcaca cgttcactat actaaaaaac gttgacaaaa acacaacaac 180

tatactaata attaaagaag agaaaactga accaaacttt ttgtaaactc ctgaatttaa 240

attagtaatt gcacaacagg tccctacatt tgtacaatct cctctcttta aagactctct 300

ctctttctct ctccatctct atcttactct gtatttctgt cgtctgagca ctcaatgaaa 360

ccactgtaaa tttccgccag aatttgatgt gatggaacga taaaaatcat ttttctcgg 420

ttaaagtaaa aaaacaaaaa caaatttctg tagaaatcat aataaaagaa agaaaaaaaa 480

tctaatgtcg gtacataata cggttct 507

<210> 33

<211> 2000

<212> DNA

<213> Glycine max

<400> 33

catgagcaag taagtaaaca ttttatctct gttacactcc aaacacatac actaacttaa 60

gcagagtcct ttttcggcta ttcactccat caccaaagaa tgagctcatc cataaaaaca 120

tatacatgtc aacatgtcga aagatgtaga acctggtaac aataataaag aatgataatt 180

tttttatttt taaaacgaag cactaaaata gatttaatta tatttatcat ataaaattac 240

caattaatca tatatctagt gaaatgatca cttcaagtat aaaaaaagat tttatacttt 300

tcgtctttct gtatttagaa atattagcaa agtcttggat aaaaaagtgg tcgtagcctg 360

tccctgtagt ttccttgtta gtcttgatga acaagaagtt ctcagttctc cccaccctat 420

tctgattcgg tttacatgga agtagtaagt aaccatacac cattatagaa ataatacgat 480

aaccacacgc catgtcttac ctcatgcgtt tactgaagtt gttttcttct tttatttttc 540

tttgatggag ttatggtatt aatattcaat attagattgg aacttgcaga tcaacttcaa 600

gaggcactct ttgataagga taccccaggc attgcttttt gacataacgc caaaaccctc 660

ctaaaaaacc cttcattttc tatctcttag ttccatttta tgcaatgaaa taaaacttct 720

caaatagtgt caaagcccga aaattgccta ccatatattt atcacgattc atgaagggta 780

tcttaattcc tttttttttt ttttcctgaa atgtttttttt tgaaggaatt ttcttgaaat 840

gttttaatgc cttttttttt actcaagaaa tgtcttaatg cttgtttact tacataaagt 900

aatatcgttt gtcttttttt acgcaatatt atattctagt actctgtctc tccaatctta 960

ttattttttaa aatttttctt ccttcctatc ctattatgca caaaaaggtg taattttaac 1020

1080

1140

aatatgatta attattttac tttcatctta acttagcaaa tactttctga tcagtgcctt 1200

atctcgcaca atccacaaac attatctcgc acaagccaca aacacgcgct tcatgatcca 1260

aaattgtacg agcgacgctg tccatgtctc ctagaacgcg cgtagtaaga aataagtgtc 1320

cctttgattt catgttgcat agttaatttt tagtttaaga ttaattttag atagttttcc 1380

atatttttaa ttttatatta aagataaagt caaaattaat gtttagaatt aattgagttt 1440

aagtcatttt aggtacctttt tggataaaga aatttaaatt aaattttaat tcaaaattaa 1500

1560

cacattcaaa gctccactaa aaattgtctt tgattaatag ccgtggatga gatttgattt 1620

attagtgaga aaagacaaag aggtttaagc gcacgcgaag agaggcgcgt aagtaaatag 1680

gagaaacttt agctgtcaaa tatgctggga aacggcgagt acgaatgacg gcggctacca 1740

cccttatatt acagtgacag tctcactctc acctatctag cctaacgtcg cttcaccgcc 1800

gtttcccatt cttattctct ctcttcataa cactcttcct atttacagtt cacgccaaat 1860

gcctgcactc tttctctact attaccaagc attggccaca ccaacaccaa cgaataacct 1920

ttgttattgt aactaataac cactgcattt ttcccatact cgttgatctc ttccactaag 1980

tgctgtggtt ggtgaccgca 2000

<210> 34

<211> 2479

<212> DNA

<213> Glycine max

<400> 34

ttggattgtg tagaagaaaa aaattgaaaa aaaatatgac atccatgtta atttttgaaa 60

ttttcttata ataatttttcc gtcttcaacc aaaccagact cctattaagt atagtaaata 120

ctattatatt tttgtggact tcttaataaa tagtagtaat attttgaaaa gcttttttat 180

ttttacgaaa gtcataataa attataaaaca aacttataaa aaatattaaa tcttatagta 240

300 ctacttttat ataataataa taataataat aataataata ataataataa

tcaaaattat taatattcct cttgaagttc cgtttcatat tctgtaaaaa aaagttgcgt 360

ttgatattaa aatataacag tactaaaaaa acaacataaa aaagaaaatc atgtttgatg 420

aaagaaaata caaatatatt ttcataaaga gaacttcaca attactcgca atgctgtgtg 480

aaatagggat ataaccttta tccaagacac gttcccatca ttgaagtata attaaatctt 540

ttacggttaa ttataatgaa atcattttgg atttgctttt gcctattatc acttttcaca 600

660

tctgcttaga ttttttggca tagttaatgg attttagcct ttttctttct tattaatttt 720

tttctttcat aagcatagtc ccggtaaaat tctcactttc agttgatact ttacctcctc 780

cgaaaagttt cccatattag agactcaatg gcgtataaaa tcatcttaaa catttactta 840

taatgaatgg aaataaaatc taaaaagtta gctactaatt ctttcacggc cattacgaag 900

actttgctta aaaatggaaa aaaagcaaaa tataaaagag tgtacattgt ctatttttat 960

1020

1080

gttcgatcct ctaataaaac taacatttta ataaattaat

tttgagtttt ggaagtgtaa tgagtcgagt aattttattt gatggttgtt tggttcactt 1200

atctatgttg atcaagtaat cgatcaattt atctccatga ataatgatga tttttaagaa 1260

tatttaacat ttgaccatca attccttaaa tcatgtaatt atttttgtca accatgcaac 1320

1380

ttattaaaaa aaacagagat gatgaactcg tgataaagaa tcacctaaca cattactgat 1440

actctctata aatatcacat gacaacctta aacaaatacg cacaattcat atatcaatat 1500

ccattacttt gtcatattct aatttgagtg taaaagtctt tattattaca gtcttttaag 1560

ttggtttaga gcaatttgag ttaatatctt tatacaaaaa taacttaatt ttttaatatt 1620

1680

tattatcttc atttttattt ataaaattta attacctaat tcatcaatat taaaaaaata 1740

aattaattta attaatatca ataaatttat cctaaactta taaacattat cattaatgct 1800

1860

attaaattaa tgtaagaaat aatacaaatt gaatattgta taaaggaaca aacataattt 1920

tgttttgtat tagaccataa gtaatactcc atattagatt atatatataa cttttatttt 1980

2040

2100

aggagagagg gagagagaga gaatgtaggc gcgtgtggca cagttatgag ttaagactta 2160

ggagaagtac acattggcat aggcattgtt attggattat gtgtagagtc cgatagacta 2220

gaatgacggc tactagttac tactctctct cttcataaac acaccattta tgtttttccc 2280

ttcccttcac gccaaacgcc tgcactctac actctactct ctcgtgctct gtgactactg 2340

tcactctctc ataaaccaaa catgccctta atccattttc catagtagtt agtgttgtta 2400

ctcatctctt ccatcttcaa tctctcttct ttccttattg ttgctcacca aggtggggtt 2460

ttttgtacgt gtggtggca 2479

<210> 35

<211> 2000

<212> DNA

<213> Glycine max

<400> 35

cattaaataa ttctaaaaaa gatataaatt tttgtataca aatctatatt taagaaactt 60

ttaatctaga tgtcgatttt aaaaaatatt atttaattaa aaaatattag atggtgtaat 120

180

catccttagt ccctaatatt ttcaattctc attttgttac cagacttgta ccgaacaaaa 240

acaaaatatc taatcatagt tttcattcaa caaaaatgat ttttaactca atttgaaaca 300

360

acattctacc atcattatta ttgtttctac taccatcatt attaatgtta ctactatcac 420

480

gttaatatat ttatttttgt tctaaaaaat tattattttt tcatatcttt cactattttt 540

gttattattt tagcaagttt gattattttt tattttaaat atttttatgt gtcacttttt 600

atatcacatt atttaacagt gtgaatcgat aaaaaatata ataattatct ttaatttgta 660

agaatttttt caaaattaaa actgatttta gttcttgaaa aatgtaaaac taaaaatgaa 720

aaccacctaa cggggcctta gtaattagga catggtctcc ctggttaccc acgggatttt 780

ttcacatcaa agaagacctg gtattttcat tttcatgaga tttttgcata tcgaacaagg 840

cattaagaca ggggttgtca ttgtcgtgat agtataattt acatggtcga agtgatagaa 900

actttaacca tcatttacct tgtaccttac tataacacaa aatactacga tttccaaacca 960

ctagatcgcg cgcttatgtt ttcagacaca ttattcttct tcattcataa ataaatttgc 1020

agctagtata tgataattgt accaatttat gtaagttttt tacaaaggac attcttatct 1080

1140

attaattaaa attaaaagag gatataaaaa atatgctgct aacatcttga acatttccgc 1200

aatcaataat ttctcaatct atctgaatat ttttgcaact gtatacaaaa atctcagaac 1260

agaaaattat tgattaaact ggaagaattt aataacattt gattcacgtt tgtttagtga 1320

ttaaaaaatc ataacattac actatctaac aaatgcagca tccataacta ccaaacatta 1380

aacaagagaa acagacaaag tccaataatc acagagacac gcagtgacaa agaaaagaaa 1440

gagggaacgg taaagagaaa ggtgtctctg tcatctcaaa tagattgcca taactccctc 1500

cttctctctc acaagctctt gcagagtgaa agcgaccact ttccgatctc aattaaaagt 1560

atggcataat ttgcaatggc ggaactgaac gaataataat aagagatacc atagttaaga 1620

1680

ctatggtgca gtgttgcagg ttgtagcaga agctctgcca aatagtgtta actttattcg 1740

1800

gtaattcagt aatgttgttg cattatagag agagcgtggt ctatgtgcca gggtgatgtg 1860

atgtcatttc actaccttca aagccagaaa aatgcaacag aaaaagcttt catcccatca 1920

catcatttga accatgaatc atgaactagt tttctaaact aaaactataa caacaccttc 1980

ggttgttgtt gttgttggct 2000

<210> 36

<211> 2000

<212> DNA

<213> Glycine max

<400> 36

aacatttaag atcttaaaga tgccaagagc ttcatatgaa aatgtacaaa agagatttta 60

aaggcaatat caatgctgtg acgccatatt aaaataaaag ggatggtttc tcctgtatat 120

tgagcaattt gtattactta tatacacaaa atctaaattg attcttaaca aatatgtaaa 180

gaaattaata atatgatcaa gttacctgaa gaagctaaaa taaaatagaa aattaagtaa 240

aagaaatgag gagtagaata taagataaca tcaaaaaatt atttcagcat attttaagaa 300

catcaaattt acctttcatc aaaattaatc ttaaaagact aaaacattta attaagttta 360

420

ctattgcctc aaaatttgca ctaaacaaga gaccctagag atttcgttag aacaataata 480

gacacggtat taaataatta aattaatacg aggatgcata actaccaaac aaatgcgata 540

aataaatgag acgacgagag agcacaacgc gggaatgaga taattaagaa aaaaaatcta 600

ataaattagg aaaaaaaaga cataatatca taagcttgaa tccaatgtac aaagagaggt 660

tggcaataaa gagaaagaga aaagacgtcc ctgtcacctc aaatggattg cattactcat 720

tgaaaaggac attattactt ccgacttttt atattaactt actaattata aaatatataa 780

aaaaatactt caaagatgca tatattttat ttttattacat aattacataa cagaataata 840

taaaataatg taactacaca ttaaaacatt aaaatagtga ttggagtagt ggtataagag 900

gacgttgaat tcacgcggaa gagaaggata tatttcatgt ttaatttgtt gtcatgccta 960

1020

1080

taacataaaa gttcatatta aactttttcc tacatacttg gatcaaaatag tcacgtattg 1140

caggtaaaaa ataatagtgt agcttataga aatcgtagaa ataagtctat aaaccagaag 1200

1260

atgacccatt tccccataaa ctctttgcag ataatgcaat ggcaaaacca cacagaaagt 1320

gacccctggg aatcaaaagt taaaaccaat ggcacagcat agcacagtgt acagtgttta 1380

tttactatat agcaaaacac tcactggcat aacactttag ggagagagag agtgaaaaca 1440

agtgtaaaaa gagagaaagt taggagggggg atagagtg tgtgtgtgtg cagagtttgc 1500

aggcttgtag cagaaatggt ggcagatggt tttaacttta tgtgtgaaat aattttcttc 1560

1620

ttcttgtgtt tagggagtgt gatgttttgt ggcagaagaa cgatgtgatt ggacacagcc 1680

aaagctgtgg acttgttctg ttactacttt gtaattgtaa tcacataaaa ggctagaggg 1740

tatgaagagt gcacagaaaa atactagtac tagtttcaaa caaaactcac cttactacta 1800

cccttccatc tcaagccata gttgagttga gtggtgcaca gtgtcactat acataccact 1860

aacacccttt tttggttctt gttctgtggc tccttgtgct ttgagcaaga gctttttgag 1920

aaagagcttg gtggtggtgg ttgttgttga gtggtttcat ggttaggctg ttgttaagtt 1980

gaagttcatc agttgcagct 2000

<210> 37

<211> 1399

<212> DNA

<213> Solanum tuberosum

<400> 37

aacgaaaaat ttagaaacta ttagtgatcc aaatgttcgt gattacctgc aacgagaaca 60

acaacgaata cttgaaaaaa gaaatcgaca atcacaacca caaccataat cgcaacaatt 120

ctcagaatca tatcctaatt ttttttcgaa tagtgctaaa tttgaaaacg acctaccgaa 180

tttctaaatt attgttgtga tcaattaatt attatgttat gtattgtatt ttatcttgta 240

tttaaattat tatgttatgt attgtattgt tatcttgtat ttaaattatc atatcatgta 300

360

attttattat tcacgaaaat tagaaaaaaa gttaaaatac tattaatttg aaattaaaat 420

agtatatatt aaataatatt tttaaaaata ttatattata tttaaaaaga attatgaata 480

ttagatattt aattaatgga attatatgta aaataatatg ttaattagaa agtaatagaa 540

aaataataaa ataatgaaaa agtagaaata gagagtgtga atagtagaat ttggagaact 600

660

ctctatttta ctctcaaaat atagagaatg gagagaaaaa tagaggtgga ttggagatgg 720

tcttagtgac atttttgatt ccgccaatgc tcagttggcg tagtcgctgt caaacttgag 780

aaaggattac ccctttaggc ttgcacagac agtgacttat gatgaaatga agccagagaa 840

ggcactctgt tataacactt aaatgaaaat acatgtgtat ggactagcaa taaaaggggc 900

actagtaatt ttagtaattg aaaagcaagt gtatagagag agataatgag agagaaagag 960

taagtacact actactgcta ctatcccata tagctgtaat gttgcaggtc tgatttttgc 1020

agttgcagac ccccttcttg gcacaagctc ttttaacttt tatcttctca aataattctc 1080

tctctctctc tctctctttt ttctcttttt acattgtgag gaaactgaat acccattgta 1140

tgtattagtg tgaggcctat ctgccacaag gatgtgatgg aacactatgc ttcctctgct 1200

aaaaccccac aaccccaaaa ctctttttca cttcacattt aatcacaatt cctcagtgaa 1260

attattctgt tgctctctct aatttcaatt tcaatgtcgg taagtccaag aactggtttt 1320

1380

gagtctcaga gaaactacc 1399

<210> 38

<211> 2000

<212> DNA

<213> Solanum tuberosum

<400> 38

accttata agttacaatt tagttatgta tataagttaa aattaaatta aaagacattt 60

cgaaataata tgattatacc atttcgaaat taattagaga gagaaataag atctcgcaaa 120

attaagtgtc ttcttgaaat taagaaccat tttttaggaga taattatgta ttttttcatt 180

tttaatttga cacgtatgca tatccactat tttgttttat tccaaagtga cccctacttc 240

ttttggtaat ttctttgagt attttaaact ctagtccccc tttctcaagc aaaaaggctc 300

actcgcgcac gcgcgaagag acattgtgac gcgctggatg gaaaatccag aagcgtaact 360

gtcaaaaaat agaacaactt tgggaaacgg ggtgacggcc gctgccacca cttttttcat 420

ttccaaacac tcattaacta acgtcgtttc accgccgttt actgcttaat gagtatgaat 480

tacactctaa tagtctattt ttacttattt ttaatgtgtt tatcaaatta tatttttaaa 540

600

660

cttgctaaat taaacaattg aaactatatt tatattaata aattgaactg acaaaaataa 720

ataaaggaac tatatatttt ctcaattata tctttttact aaaatattat ttttctaata 780

ctagttaaac ttttaaaaaa catctaataa agaaaaagaa tttgttcaat tatactttag 840

aagcttttat tattattatt attattagta gtagtagtag tagtaataaa ttagattaaa 900

ttaaagagag aagtattcaa aactcccaaa actattgtat tagttttatt tcagaactat 960

1020

1080

tttactagac tctttattaa gaacttcatg ttctttcaag agtttatgag cacttgctat 1140

gtcatgttac agatcaagaa tatctacaga gtgtatctaa atttagtact agtaaagtag 1200

aaaatgtatt acttatctct caaacaatag gtattcatta tactattttg agatgtccaa 1260

caattttttt tcactttatg aaatcaatga ataatttaac acttagttcc taattcccag 1320

1380

1440

1500

tgtcactaaa tgaaaatact tcatctgttt caatttatgt gatagttttc atttttcaaa 1560

agtcagacaa ttatatattt ataaattaag taaaaaatat tataagtcac actaattaac 1620

aattcgaaat attcggtacg gaggaactaa cacttatgtt tttagaccat attagtcttt 1680

tctctctatt tattatataa tattgagagg agagtgcaac caccatggca actttctctg 1740

tcttcataaa acgcagctga cattaaaaac acagacacac acttcgcatt tcatatccct 1800

ctcactacac gccaaatgcc tgctcttcct atttctcttc ttcttctttt tcttcttctc 1860

tctcattcac ataacacaca ttcttgtact aactctgcat cataaactct accccacttt 1920

cttcttcttc tccggtcata ttgctctgaa actccactta ttgctctctc ccggcattta 1980

tttttagtttt ctcagaaata 2000

<210> 39

<211> 471

<212> DNA

<213> Arabidopsis thaliana

<400> 39

taataagaga cgaaaaaaaa ataactaact gatcattacc atccataaat aaatagttgc 60

tgccataaac caaacacatt gtgcttatca aaaagaagaa atttgtactt aatgaaacat 120

tcattattag caaagtgtaa aaccaaagaa aaacaaactt

aaagtgaaga agagtaaaga aaaagagaga ctgagatgag gctgagagcc tgagtctgcg 240

ggtggagaga gagagaaaga aagcctcttt acacgtgatt tttaaaagag accaaaaccc 300

360

acatcacaaa atcaatcttt ttctcttctt cttcgtcttc atcatcatca tcatcatctt 420

cctctttctc ttcctactga gatattttct ccacattgag aggaaagcta t 471

<210> 40

<211> 1231

<212> DNA

<213> Arabidopsis thaliana

<400> 40

aggataaatt tcatctatta agatatcagt caattataat gtgttacgtg attcgataaa 60

120

ttttggcatt ttgccaaaac acataaagat ggttagtgat gagacgagac gagtcatgcg 180

ctacttttaa aacaaaatga aaaacatcat taagctaaca aaccaaacac acttgttttg 240

ataacatgtt ctagggaact agttatgcca aatctaatcc gcataagaag actaagtcac 300

360

gagaagagac tcttttgatc aacacaatca aacatctaca aagaaaatta tctcacatag 420

ctacttctta atctaattttt ttcattaatc cattttattt taaatgtgaa gaatcgcatc 480

tagatgtgac ctctcatgat aaaaaattaa accattgtaa aaaaaatgtt gtgtaaaact 540

aaatataata aattattaaa aaaatacaaa ttcaatccac taggttaaaa actcctatgt 600

agaacatttt tttatattaa aatgtaaata catgaatctt atttttcgaa aaactaaaga 660

catctttttt ttatatatta attaccaaaa caaaataaga cgacaaaaat attctttgat 720

atagtaaaag aaaactagaa aactagaaaa caataaatta ccaaaacaat ctagaaaaca 780

ataaatccta ctttgcatta ctttattata aaatcccgaa atgaatctat aaatgtagaa 840

aatattatac aaaagttgta agagatttta atatacataa ttacatatat atacaagtaa 900

atacaccgta tatacatacg aaggagtaaa cagtattatt tggtatatag ttacgtctct 960

atatacgaag ggttcaaact tcaaagtaat aatttaatca acaatgtgta catattgata 1020

agtagtagta tatatgtaaa ggtctcacgt ctctataata aagtatgact cgtcacgtga 1080

ccctcctcttc ttcgcagaga cagagatagg atgagacaga aagaaaccaa caaaaccaaa 1140

ccccaaaacc caagaaaaag agaaaaacac tctcttctct tctctctctc tctttctatt 1200

taagagactt cactgtctct ctcagtcttt t 1231

<210> 41

<211> 1548

<212> DNA

<213> Arabidopsis thaliana

<400> 41

ttcacgtgtt

tggagatctc tccctagttg atgaatagat atgatgaatt taattctttc atgaaataaa 120

aatatatgaa acatatgtag cagaaaaaga agcatatcta tgaaacaaca aacattcaaa 180

aaaaaaagga aaacggaaaa ttattaatat gaaaactacg gctttgactt gtagctgact 240

acatttacga catatatata taaatggacc ccactgagtg tctgcaaggt ctttacacaa 300

360

ctacatttta ttggattgtt tcgtgactct gtaatctttt ttataagaaa acaagtaata 420

gtgaaattga agtaaatagc tcagcacaga aacttcgaca aaaataactc acagattaga 480

aaagaaaata tatgcataaa tagccatggt tcatttatga acaattttatt cgttttttta 540

gtttataatt tcattaaaac atgtttgtca catcacattt catgtccttc ggctcctact 600

acaacaacaa gtcactgtca tctccattac ttccacttct gctcctttct ttattaactt 660

gttcaaaaac aattctaaga taaataacaa taaatgttgg tctctcttta ttatttcccg 720

gctaaagaag gaggatgtct cgtattatcc gccatcaatg ctcttttgtt tcctgtttct 780

tgcaatttga atccctgaga atcctagccc acttatttac tactttgcct tagctgtttt 840

cgacatcaaa attttggtca tatgactcat atcaatcttc aaatttgata aaatatgttc 900

ccaattcaca aaaacaaaaa agttttcgaa agctcaaaaa cctttaccat ttcaatagta 960

gataggattc ttttagattt gcatttcacg aaaagagaag aaaaaaaatc gaaaaatatt 1020

tgcaatcatg attttttgtt tctgaaggag acctgtagtt gctgtcatga acattaaata 1080

caaatctaat aaatgttgta cgaattttgc gtgtaataaa tggtcagggc cggctcgaag 1140

ctcgctgatc gtcctttttt cgtgtctcta tagcaacaca caatcgtatt tatttcaaac 1200

tttttttact ttgtttccca tccatcaaat ataagtataa aaatgtaaag aatcatcata 1260

tatagatcgt aaattcattg cttcctttgg ctttttattt catctagacg acgttaaaac 1320

cagaccagac caaatacatt tatcattttt cccttttttc taaaattctc tctttgattc 1380

ctatcttctt ctctttattt tcactttgtg ctttctctgt ctctcctatt atgagtctaa 1440

aagtctacta gctgttcaat agttttgtct ttctgtgttt cttcttcttc aaaaccgaaa 1500

gaaattcaaa aagagtcttt cgctgcttgt tagtggggtg aggaacaa 1548

<210> 42

<211> 440

<212> DNA

<213> Arabidopsis thaliana

<400> 42

aaacccgaac ccgaaccaaa cccgaaccaa aatcttaaat tacccgaatg ggtcttaaat 60

ttctaaatcc gaaaaacccg aacccaaaat acccaacccg aatctgaccc gaatatccga 120

180

attacggtga gaattaagcc catttacgtt acggaaataa aacacccatt taaaaaagcc 240

caacacgtga agcccatttc cgagtgcgtc ccacatttac tccaacggtc gaatcgactc 300

aaacattcaa aatacaaaaa cgctatcttt atcgtcttcc tctgtctctc tctcacaaca 360

cataacgttc aaatcctctc tctctctatc tcgtctctta tctctagatc taaaaatctc 420

ttctttcctc aatctctgtt 440

<210> 43

<211> 317

<212> DNA

<213> Arabidopsis thaliana

<400> 43

acagacattt acttatacgg ttattgaggt tgaactggac cggagtagca ataaattatc 60

ggttcagttt gggagatcaa accgtttaaa agaaaataat ttgaaatggc cacgcagaat 120

180

agcgtcaagg taaggtactc tctcttcata caacatttta gctttacttt ttctctttac 240

tcttctctct ctctttctct ttctctttct ctttcactcg ttctctctca ctcactctct 300

tcacacacag atccaag 317

<210> 44

<211> 3912

<212> DNA

<213> Arabidopsis thaliana

<400> 44

acaccaataa aaatacacag caataaaatc gctacgtata tatatatata atatgtatta 60

tctattacaa gatagtaata gagtatagca agttgtatca tctaacaaac tatgcgaata 120

aaatttgaac attgtgacat gtagatgtag tgtaatttag ctaagtgctt atcatcagta 180

acatagaccg acttaacttt ttacgaaaaa aaaaaagtaa catagaccga aaaaatgcat 240

atcgtaaatt taatggaaaa cacaatttac gataagtaaa aaacaaaaag aaattacgat 300

360

gtatgtaaat gcggtgatta atgtgatcat tagagcgtgt gtgttaaacg cggcggtttt 420

agtggagatt gatcagctga taacactctt accgggacga atctaattcc atattcatgg 480

cttgttaaaa cctaagacat acgcaatctc taatttgcta gtatagttag ttctatatta 540

tttttcgact aataatgtaa acatatgatt attaagtcgc aaaaagagtg cttaacaacc 600

660

taccaagaat agtggcgaag cactataaga gcagagaaaa gaagctcaat aatgtactaa 720

aagttgtaga tttttacagc ttaaatacac caaaattaat agaaaagttg gtaatttttt 780

aattcatggc tactgattta gattttagaa aacaatagta gtatcattgt cacatcttaa 840

acacacaata ggtatgtttt aaatcaaagg ccgtagttaa tttgtcaaaa atgtatgcat 900

ttggtatttg gatgtctccg aaaggatgga tatatggact tgttagataa tttcatacct 960

cagtatcaat agtcatggag cccaaattgc tcaaaaacat atttttaatt ccaagacttt 1020

gatgaagacg taataatgag tccaatgggc catcagatac aatgttcgga atttaacggg 1080

tttgttagtt ataagtattg ggcttgacct atctggttca atgatatgta ggaacaaccc 1140

aatttgcaaa gctttattaa aagactcttt agttgtcgtc aaggtttaac ttgtagtagt 1200

tggtaagaaa ttctacgtga aataggcaac attacaaaaa caaaaatcaa ttcgaaatca 1260

tacaaaacga aaccaagtag taaccaacta cactattatg acattaatga ttagacattc 1320

ccaaatcata caagttcctg tcatgaagga aacaatggtc cgtatttgca aacgattaca 1380

aaaattcaaa ccaaaaatga aaaaacgagt taaattattt ggtttataaa aatagtaatg 1440

tcaacagaag actagattgg gaaacctgaa gcgaacagag cttttaaaaa cgagtttgaa 1500

cggctgggat catttggtac aatacccacc gtaagtttgt ttaccctagg gatgcaagcc 1560

aaaggcccaa atcagttact acttactgct acaaccatcg tctcagcttt ttgtctcagc 1620

tttttactaa tgaagcatac aatttcttgg gcatgtcaca tctcgacacg tgtccactat 1680

tctcttctct tattggctac tcgttcgtag gcttctgtta atagatgatc tctctataac 1740

tctaacagtc ttttctttct ctttatttcg ttttggtatt ttaagtttca aattgaaaat 1800

aataggagga aaagtctagt tttaaatatt gtttttttac aagtgaacgt gaaccaattt 1860

acctcttttt ttttatatat cctatcggct aatctggtta gtatcggtag aaatgcaccg 1920

aggtgctaca gagattaatg ctagggatag tcagaccgct tgtatttctg actatcaagt 1980

2040

tttttttttt ttttgtaaca aatgtgattt tgttttcaag gaaaatagaa cttacgtttg 2100

ggaatttcac ccttcactaa agcttccttc tgccattaga ccacaaaggc ttgggcaatt 2160

taccatttttt gtaaaagtag aaaacaaaat gcctaaaatg ttcatacttc attacatcaa 2220

2280

taataagtat aaactctgat tattaggtaa ataaattact taaaacgatt 2340

actcaactga caaaaccgta gttgaataat aaggttacta tgaataccga ttgaatattg 2400

caaagccgga attgaaaaat atataacaga tcaaatgttc aagtgtggtc ataattctca 2460

cataggtcat atagctgaac ccatgcatct atttactagt ctatagaaag tactagagac 2520

2580

ctccaacatt tattagaacg aataaaacca atatgatgat gattagttat tggtaaacat 2640

ataaacgttg agtaaacttc aaaatagatt gaagtactat taagacttgc attttttccc 2700

cttgggttat attcttgaat cgtttcgaag tattttaact ttcaagaata gaaggttcct 2760

caactataaa caattacatt aatcaaaacc atttctatgt aaacaacata atttttgtat 2820

2880

acaacaaaaa ggactctgga gacctaaaga tccaaaacta tgcaaaataa agatacggtc 2940

ggaccaattt aatctaacaa aaccaaatcc ttatactaaa ctatttaccg atacatttcc 3000

3060

3120

aactatggta tatataaaca tgactctcat ttgtatttat cgcaaggtac aattccacca 3180

atttttttcg tcccactcat acagctttaa ttgtgaaatc aatccataaa aaaccaacat 3240

gtgacatggt ctctataact ataactataa gatagtaaaa aattcacatc aacataaaag 3300

3360

aaccggtcca accggtgtcc ccaatcacta tcaaagcatt aactaacttt cacaaggaaa 3420

agcatagttc agtttctcta catcgcttcc catcctctta accctgttta ctcgaatcat 3480

ccaccgttgg atcaaacacg cgctacaaat ctagcgcgtg accgaggtttt ttacacagtg 3540

gaatattacc atgcattgga aagcggcgtc tacaacaaac ggcgggtcat gtcaccgtca 3600

aaatcaacct ttcttaattc ctaacgccgt tacttatctc cgtttactaa aaatgttaat 3660

gcgtgtgaga gtgaagatca tatactaatt agaagtggct aatgttttaa cgtgacatta 3720

3780

taattgcata catatatact ctcacactct gacagatttg tcgtggtctt agtattctct 3840

ttcatggcta gttatatagg gctctagtac attatctctc tctccccatt tctctgtctc 3900

tctcttcttt aa 3912

<210> 45

<211> 2000

<212> DNA

<213> Arabidopsis thaliana

<400> 45

atctttatgg tcaccgagtc tactgatata attttactgt cgcagtttgt ttccactact 60

120

agattagttt aattagttta gccagtataa tgttttagta aagtattaaa cggcattttt 180

cgttgggaga attatgttat ggtataatct actaatacat acttttacac atatatcaaa 240

aagtttgacc atagtaggta gtacaacata gaagaatcaa gatcggaacc agcaaggaag 300

aagatacggt cggtccatat taagctaaga ggaccaacgt aactcgatat atatttttct 360

gttctctacg ttaccaccat ataaatttta atattgaaaa aatcatcttt tggcattgtg 420

tttgatgtcg gattcggaat atggaaagag gagagatatg agattttggc acaaaggaag 480

ctgccaaagc attagggcaa ccgagtagta acgagatcaa acatcgtttc aatcggacgg 540

tcggggtttg accaatattt ctcggatatc ttttggaccc tacgttctga cttgaacttg 600

660

gttagcttca aaacaaatac taatattcat taactatgga tcggcatagt tttcatgtaa 720

tcagctgagc gtttatcata ttgattgaag ctaacatgta aaattctcat gatcacattg 780

acttttgcct acaaatttta aaagagtata caaataattg cttaatgaag atagcttcca 840

tagagaaaga gtaacagctt tatacgggagg catagcttta gacacgatct ctgctcttgt 900

gttttttgtt taacactgaa tccacagtga aattactgct cattttttttt catttttat 960

tacatttttt tttttacttt tttatttatt acaatctaca gttctaccaa cttattcaac 1020

ctagtggtac catatcgacc ccaaaattaa tcaatctaat tacaaggtag aaatagaaag 1080

1140

taaaaaatat tgaaagctga agaaaaattt tcaaatcgat aaaaggatag tactaaacca 1200

atccggtttg tggcatcttt ttcacccaat cactatcaaa gcattaacta aaaatcacaa 1260

ggaagagcat aatttgattc tctacatcgc agtccacgat aggatttctc tatccaccgt 1320

tggatcaaat ttaataatga tgcacgcgcg cgtcaacggg attttaccca gcaaaggaat 1380

gtcttttcac cggactctta aaagacgttc ttcttttttc acctttgcat tggaaaacgg 1440

cgtttcttct tagaaccgtc gccgtcaaat catacggcct aataaatctc cgtttaacgc 1500

cgttacttta ccgttaagta ctaaaaaaac aaaaaaaaat catttcgatc actgtctcat 1560

taagatgatc ggagatgttt tagcaggggtt taacaagtga tgatagtaat gtatgtatat 1620

atgttactga cattattttg tcgttgtcta ataggagggt actaaagttt ctctctctca 1680

tggcgtcgga gctcagcctc tagtaatgta gactgtcctc tctttctctc tctcttcttt 1740

aaacatctct gctctgtttt ccttccagtt cacgctaatc tcctgtgtcg gtcccctctc 1800

1860

1920

aaagaggtct ttaaccaaaa aaggttgtta cttgttttct gggtttcgtg gtgttactct 1980

tgaggaagaa gaagaagaag 2000

<210> 46

<211> 1470

<212> DNA

<213> Arabidopsis thaliana

<400> 46

ctacctaata taactagcta gggatttcta ctcttgtttt cataatcgat ctacggacat 60

ttctcggaac gtggtcaaga ttcatgagtc ttctgttttt tatgtctctg ttcaatttgg 120

tttagagatt agtatgctta tttgtttatt tcatatatgg ttatgagagg agaggctaat 180

ggcatatact ctgatgtttg tgatggctgc taatatcgtt gaggagttat tcacgttgtt 240

tcatgcgcaa aaatcaacag aaaaaattct gattatgagc caactctgtg aacccttata 300

gtgcgcccag aggtttgcga ggcaaaatcc cgatgaacca gaaggaattt tagatctcta 360

tcaacaataa ctatgatgga gctcgtttaa attcatcaca gcgacaacat cattaggctg 420

cccaacgtct atgtctcctg gaggtgatgg tacttgatct ctcaaccaat tttcttgaaa 480

atatcatgcc ttgtgagcgc tttcatattg cgcctaaaat acccaatacg caatgaacct 540

acttccaaag gcatagaaaa aaaactgata atgataatga gatttgtcac tatacttatc 600

660

tgctatataa cttttcttta tcagtctatt gttatatgac ttatatatat ctcaagagat 720

aaggccaata aatcttcttc ttaattatat ctgaagactc aaaacatatt ttgagtttaa 780

taaaataaat aacgtccaaa tgctacatac aaacggacca aattcatgga ggtataaatt 840

taaattattt tttgttccaa agtgtatgca gtgatttatt gatgaatgcg atagagcggc 900

gaaagagaat aatcgtcacc tagaagacaa attgatcggc cgtacatata tacataaata 960

caaacctgcc acttcacatg tcacccacct ttaagcaccc ccttcacata catactttct 1020

ataacaaaaa tatcagcttc tagttcatat ttatgttaca ataactcgag tgaatcatac 1080

taaaaaaatg taatgctttc tctaaatagg agataaaatg caccctccga cctaactaaa 1140

gattccttat tttagctatt taagacatat tgcacatgta tagagataca taaacacata 1200

1260

caacacaagc gaccaaagcg atgcgtttcc caatgataag gttacgacat acttacacga 1320

ctctctctat tgtctcgtct ctttctttcc tcatccctct cctttgtctc ctttcactct 1380

atttttcact tttcagaata cttttacgta aaaatcatgg acatgtcatt gtctccaccc 1440

tactatactc tttttttgtt ctttttgttt 1470

<210> 47

<211> 1793

<212> DNA

<213> Arabidopsis thaliana

<400> 47

ttggttgtct ggcatcatca ttttgtaccg ttttctcccaa agtaagaaac ggtacaatct 60

tctcttatat agatttcata ccccaaaacc ctaaattcat tagggttttc aaaaaaaaaa 120

180

tttcagttt tctacttaac caatattaaa ctaaagtcga attgagatga gtggtagcaa 240

accacagttt aattaagaag ttaattatag gccacatgat tgagcaagcc ttttgtttt 300

gtaacacatc ttatcagctg cttaaaattt tggctgcctc ccattggcca actggtctaa 360

acatcattgc attggcattc tcataatcaa tcaatctaat gagaaacttt gaatatttat 420

480

aaaacacttt aaaaaacaaa aaccaaaaac tcttaaacta taaactcatg aacacttagt 540

gatgaggtct gaaagggtgt aaccaccacc tgttgtcaat aggtgacaac ttcttcttgg 600

gaacattcgg gaaagtgaag gcttaggtga cggttgtctt aaaagtctct tttagttaat 660

tcatcgtatt ttcgatgggc attacgtttg atgcataaag gcccatatgg gctatacatg 720

780

actcatggag atctactctc cacgatattt tctgtaaact tttctttttg ttgattagat 840

aaatagaaaa ttgtgtagag cgaaactttt aatgaattaa aatgcggaag cgattaaagc 900

atgaatagat aaattggaca aggattaaa cgagggatca tctagttttt acactgatca 960

ctagtcatct gcttgcagaa gaagtatatc attaatcaag caaaacgagg gcataaattt 1020

cttacaaata acttttacag taggttaatg attttttaat aacttgtcca tttcacatgc 1080

atgtgtatct ttgtactata catgctaagt gtttcattaa tcaagataaa cgtgtctacg 1140

aataacttaa aacagtacaa cttccctaaa aaattcatta aatgaaaggt ttttatagat 1200

tatacattgc acggtacggt tcggttacca ttcgaagtct aaaaagagaa tgacggttct 1260

gataatgctt ttaatcgctt ttgtattgta aatcattaaa acagtaagcc ggataccgaa 1320

ttactaatca gacccaaaaa agaatctata ggaaaaatat caactgaaga gcgggtaggc 1380

ttgaccttga aaggaagaat ggtgagcgag cggtggatag atatgtaata aattgtaacg 1440

ctttcaaaat gtcaaagtca caagtcacat tactcacgag ccaacactaa ccatgcaact 1500

1560

cataattggg tccaccact cacaggtcca cacataagga accgaaaaag gtaaaattca 1620

aaaacttaca aagtttttta gagatgatgt ggtgaagtat tgcattaatg gaataatggg 1680

aaaagaaagt aattgcaacg tacgtataga ttaatccatt gacacaaatg aaaagtttct 1740

ttctatttaa tgtacacaac aaaggttctc ttcagagtaa tttaggggaa aaa 1793

<210> 48

<211> 257

<212> DNA

<213> Arabidopsis thaliana

<400> 48

acacaacagg tccctacatt tgtacaatct cctctcttta aagactctct ctctttctct 60

ctccatctct atcttactct gtatttctgt cgtctgagca ctcaatgaaa ccactgtaaa 120

tttccgccag aatttgatgt gatggaacga taaaaatcat tttttctcgg ttaaagtaaa 180

aaaacaaaaa caaatttctg tagaaatcat aataaaagaa agaaaaaaaa tctaatgtcg 240

gtacataata cggttct 257

<210> 49

<211> 1787

<212> DNA

<213> Arabidopsis thaliana

<400> 49

aagggatatt taatgggaag aaaagaaggg tggagatgta acaaaggcga agataatgga 60

120

gttaagtttc tacttttcct tttgttgctt actacttttg ctcatgatct ccatatacat 180

atcatacata tatatagtat actatcttta gactgatttc tctatacact atcttttaac 240

ttatgtatcg tttcaaaact caggacgtac atgtttaaat ttggttatat aaccacgacc 300

atttcaagta tatatgtcat accataccag atttaatata acttctatga agaaaataca 360

taaagttgga ttaaaatgca agtgacatct ttttagcata ggttcatttg gcatagaaga 420

aatatataac taaaaatgaa ctttaactta aatagatttt actatattac aattttttct 480

ttttacatgg tctaatttat ttttctaaaa ttagtataat tgttgttttg atgaaacaat 540

aataccgtaa gcaatagttg ctaaaagatg tccaaatatt tataaattac aaagtaaatc 600

660

gaaatttttt aacaagaaaa atcaattagt cctcaaacct gagatattta aagtaatcaa 720

ctaaaacagg aacacttgac taacaaagaa atttgaaacg tggtccaact ttcacttaat 780

tatattgttt tctctaaggc ttatgcaata tatgccttaa gcaaatgccg aatctgtttt 840

tttttttttt gttattggat attgactgaa aataaggggt tttttcacac ttgaagatct 900

caaaagagaa aactattaca acggaaattc attgtaaaag aagtgattaa gcaaattgag 960

1020

1080

1140

tcatctacaa cttaataaga acacacaaca ttgaacaaat ctttgacaaa atactatttt 1200

tgggtttgaa attttgaata cttacaatta ttcttctcga tcttcctctc tttccttaaa 1260

tcctgcgtac aaatccgtcg acgcaataca ttacacagtt gtcaattggt tctcagctct 1320

accaaaaaca tctattgcca aaagaaaggt ctatttgtac ttcactgtta cagctgagaa 1380

1440

agtgtaaaat agggtaatag agaaatgtta ataaaaggaa attaaaaata gatattttgg 1500

ttggttcaga ttttgtttcg tagatctaca gggaaatctc cgccgtcaat gcaaagcgaa 1560

ggtgacactt ggggaaggac cagtggtccg tacaatgtta cttacccatt tctcttcacg 1620

1680

tcatggaccc gacattagta cgagatatac caatgagaag tcgacacgca aatcctaaag 1740

aaaccactgt ggtttttgca aacaagagaa accagcttta gcttttc 1787

<210> 50

<211> 1731

<212> DNA

<213> Arabidopsis thaliana

<400> 50

atttctctta aaataactaa attatatgaa gaaaaaacaa gagagataac gacgatcttg 60

atctaattag gaaagttttc taatgatggt aatgtgaaag agtgcttctt tcactcttta 120

tttaaagaag agatattttg gaaatagcgg tttgaaagaa agctataact tggtattgggg 180

gaaggaaata aaccagagtt ggtttctagg atgtgttgtc gacttctcat tggtctgatt 240

agacttccat tgcctaatgt cgagtctatg ttttctcgta tataattttc aataagtgga 300

acattgataaaaaaaataac aataattgat tatcgacgtc

agctagacaa gtgaaatatg cacagaccac agacttcaga gcttccccaa gtgtcgcatt 420

cgatttgcat tgacggcgga tatttcttaa ataattttct ttcattttct aactatatta 480

aaaatgtaat aatgtaacca agtagttata 540

gaggttttaa ttgctcggcg gtaagaacag tgaagtacat atagatgttt cttggtagag 600

ttgaacttca attgacaact gtgtattttg ttgagtcaat

agatacagca gaagaaaatt aattttgaag tttgggttaa ttaaggaaat atccaaaata 720

caaattacca taagtttttt ttgtactact aagcaaatta tatactcaac ttttcttttt 780

gctaaaattt gtctttgatg gaattctagg gaataaatta agagaactac gtattataga 840

ttaatacacc atgatcactt taagcagacc aaatgataat aacttattga acacgattta 900

ttcatgtagt acgtacatag atccaaagat cggaataaga tacgagagaa aatggttacc 960

ccatttattt atgtggagat aagaagcaag caactaaagc aattaaaagt accaaagaag 1020

aacatttaga gcaaactaaa agaatctaaa gctcgtgccc ttggcgacac tatccgctga 1080

1140

tttagcatat atgtttgaat cgttgccgct ttcaattttt ttttttttttg agtatattgt 1200

tttgagtgtt gtatatccgt caacatatta cacatctata tttttcaact ttttggatga 1260

atttactaag ttggcataaa ttatcaaaaa acaaattggt aaatgatctg ttattaaatt 1320

1380

attatatggt gcatgcaata tctgttaact aagatgctat ttgaatgtta atttcaatgt 1440

gtcatccaat ccaatcgagt ttttttttct ttttaaaaaa acaacaaaat tcatggtcac 1500

1560

catttttacc ttatttatct cccgaagaaa ttggatcgat caatgaagga aagaaatcaa 1620

atggtcttat gatcaaagca acattgagaa attcaatcta caaaaagcag acacatctcg 1680

acgtaagaaa gaagaaatta tcatagtgat caaaagtacg taaaatcgga a 1731

<210> 51

<211> 2001

<212> DNA

<213> Arabidopsis thaliana

<400> 51

atagaaaaaa gtggttatcc aagctacaca aactttttag acttatttct ctcattcttc 60

tagagatttt taatgaaatt tgtatatact tgaaatgcat ataaaataaa taagaaaaca 120

atatctactt aatgtcctgt tcaagaatta ttaagaaatt tcactacgtt gttaagtcca 180

ttttactcca ccttctccac tggagttaga ctctactaag tcgcgttatc ctgcagagta 240

aagattgact atgcattgga ccttgtcgat gtgaaaatgt taaaaagata attattatga 300

360

taccattaac caatctattc cgtgatattt aatgtttaaa cccttcttca attctattta 420

acctacattc taaacaaggt gtcctcattt tcaaaatcct aaactcatct tcatctatat 480

ttctattttc tgccaaaatc ataccacaaa aaacatatac gaaaaatgac cacggaaatt 540

gtcttttatt gtttatttcc aaagtcaacc aagttgactg ttcttttat aatgattttt 600

ttttaaaaaa ttatatattg agctaagaaa atattaagtg taattttcaa aaagagaaaa 660

tatatttatt ataaaaatct atagtggtgg atcaaaattc tggtttaaat tattgtatca 720

attttgaggt tcaaatttta ggatggaaga gaaacgtaga atgcacttaa agttctgtcg 780

atttttgtta gtacataatc cctcatataa ccatacatat acttattgca ataaccatac 840

gttaagaata gggaccaact tcatcggtgc ttctgttaga catttcaaga atgatatatc 900

acattcatgt atctccgtat atgtattaag accaatgact aaactcagcc attgtagata 960

tagttcaggt aaaactgtca attgtggatc agtatgttga aaattgttaa ctatggttcg 1020

cttgtaacag aatataaata taataataat atataatact aatagtaata tatataataa 1080

aaaaagtatt aaaatctcat 1140

ttaaaacaac tctccaagtt ggtagtccat tgatctacaa gacagctcca cctgaaccac 1200

atccacaatg taaaatttaa ccgtacacat aaaccatgat cataagatgc aacatggtaa 1260

1320

1380

tatttagtaa gtacatattt gttatgaaag tacaaaaata acattgtaat aggtcaagct 1440

ctttgtggtg gtttacattg taataggtca agctctttat tattatttta ctatatgtat 1500

cttcttatcc attcttatat tttatatcat atatggtcaa aagaaccaat cgtatcacag 1560

cagcatttgt taatagaact ttgatcttgg taattacagg ttctatattg aaatcgcagg 1620

agccaaacca gcaacttggg ttaattatta acttcattca ccaataataa taattgatcg 1680

1740

tgtatcattt gatactttct ctttttccct ccatatgcct tttgtgtgta taaatatatg 1800

tatatgaata agttcacaaa aatagaaaat taaatcaaat cttcgcgtaa tttctcatca 1860

aactccactt ttcttctttt taagagaatc tagggtttct tctactactt cttctttctt 1920

ctttaggtgg tcaaatttcc aagttcttct tttagctctc tcttttgttt ccaagaaacc 1980

tgagaagaat ttttgtagat a 2001

<210> 52

<211> 1814

<212> DNA

<213> Arabidopsis thaliana

<400> 52

tctacagtga cgtaagtgat gtggtataaa tacggtatgt gacgtgaata catagacgat 60

gatatttgtg gaaaaaaaag aaagaaaaaa aaaacacaaa agctacatac gagtggaaga 120

agagaccaga gtggagataa agttttaagg gctgtttggg aaataaacca tttgatgtgt 180

tgtcgagtcc tcattggcta ctttttactg tgcttgtaac tcgttcgtta gttttgtgcc 240

300

gaaatcagac caaagaactc aatagatatt aaaggagaat aatctttaac tccttaatac 360

tttctacagt aacactataa cgactatata tagggtttaa atattctttc aaaatgcaaa 420

ttgtattttg gttttttaat ttttcttttt gcatggagac aagttttttt ctggttaaat 480

gcacggagaa attttaattt tatttaaggt gactttctgt ttttccttta aacagggaga 540

aacattgtgc ttacaatttc 600

taataagtac acgaaagaat ttgacccaat tgtaataaaa ccttaggaaa aatagtaatt 660

ttataattgc aaacacaaaa ataacacgga acattttaca atattttatc ctgctaaatt 720

ggaaggaaaa aaactaaaat gatgtccttt tcttcaattt caagttaagt atcgatcttc 780

tctttgctct ggaaattgat gaacctacaa agtggaagct aggctgaaat ttagctgctg 840

aaccaacaaa actcttaaca tcaaatgaag catgttctat agatcgtggc gttgatcttt 900

ttggaccaac caatatgcaa aatgcatata ttgttgtaaa aaaaatgcct atattccata 960

acttattcac ttgatatact ataatttttc ttttgaccgg gaaaaagtgg cctatttatg 1020

ggaagagaat ctcaaacgta tgatactctc aatgtaggaa aagtctctag actaccattt 1080

tcttttactc ctttagaccc attggatttg ggtagatgga tttattaaat acaatgtaag 1140

gagtttttaa atgaaatata aagatacttt aatgattaac tggtaactgg taaattggta 1200

atgtactact attgtagtca atgtaagaaa atagttacga aaataaaacc ttttctgtca 1260

attaataact cactactata atttttgtag gattaacaca tcacactaag ctattttatg 1320

actaaataag taaatataat ttcttccgaa ggttcaactt tttgagtggt tattgaatag 1380

ggaacgtgtt ccgataagaa gttaacaaaa caacattcat gtcggtccca tgactattat 1440

atgtctgact ctgtgtgata tcgatatata cataacatat attggtctga aaagtggaaa 1500

atctatttta agatttggta actgatcaat gaattgataa ctgatagtct gataccaaac 1560

ataaactagt tttgtacact aacaaaataa gaatgaaata tcaaattttg taagaattgg 1620

taactgatat aatgaatcat cgtaactgat tattttcttt taccttccga cgtaaatttg 1680

tttatgttgc tcttaattat gttcgtacct taattgatca taaacacata aattttagtg 1740

1800

atttcttctc tcca 1814

<210> 53

<211> 2001

<212> DNA

<213> Glycine max

<400> 53

tttcgtgtgt gtgaacaaaa taaatgatct gcacatgtat catggttata gtaacaagtc 60

ccagctccca agggtagtag ctgcttaaag acacacggaa accctcgagg ataatggtgg 120

aggagagtg ttgaaatttc aaccatctca ctttttttctt ctaccttatt ctttagccga 180

aaattaagtt ctaactttaa aatacagatt tgcttcagaa gttgattttt taggtttttt 240

tttttctgga agaccaatat taatttgtga tagttagtta aaaaaattat attatgtttt 300

gtgatagtta gttgaaaaaa ttatattgtg ttttgctact aatttacttt caaaatataa 360

atatccaaat atatatataa atcttatcac ttgaaaatag atggtgggta tatggactca 420

480

gaaaattcgc ataactatat ccaagttttt cttagttcgg tccattaagc tagcacatct 540

agtagtcttt atccatggac atattattaa tttgttatgt taaaattttg atttttaatc 600

tagtggtata ttttagtatg atagatttta acttaaaaaa tgaagtcaat tttttattac 660

taattttata catttttcac ttttaaaaaa tatttatttc ataagaaata tttttaaatt 720

actatttggg tccatggatc aatttattta tctgagtttt tatgggctag atacaaatct 780

tggaaaaaag ccaatttatt tcatggattg gcttatttga tccgtctaaa atgcaagctt 840

cacaagacaa accttaaaca taccatacca tccttttatc tatctctact tcaaaatcaa 900

tttttgcaac attcactcaa tggtgcactt aattaacaaa ccctcatcca tatacacata 960

tttagagatc aacaatatga ttatctatca aaaatacaca aaatcagtgt gtgtttgggt 1020

1080

ttgattttga agtaatatta tttatgtttg gatgttttat taaaaaatta agttatgaat 1140

1200

ttctgtatcc aaagtcaatt ttaaattttt ctttgatgtg aaactaaaca tgtaaaaatg 1260

tatttaaatt aaaattaatt ttagacttat aattaattct ctgtggtcaa tccaaataca 1320

cactcacact tagaatgtat gaaatttcaa tttttaactt tctcatctat gagctgttcc 1380

tattcctcct tcccctccta tgccctcact agggagccag ccagccatat tccaaaagcc 1440

cttattatca cacatgggtc cctccatagt caaaataaaa ataatatcat gatcactgtt 1500

tggccataaa gagctatacg acacacatgg acacagtagt acactgccca accaatcaca 1560

cgtcgacagc acaccgttcc caaactactt cacctttccc aaaccagaaa ccaaaaccac 1620

ttgtcatcaa acccctgcc agatagtttt tctccatttc aatattttac ttcacctttt 1680

gaggctttgt gggtactaca aaacataaca caattgaact cactgtgctt tcccatgaca 1740

cacatctata cttgtccaga agaagaaaga tccatgaact aacattccca cacgctcgct 1800

1860

ttccttgttt ggggtttgca ttttcctgg ttgaaaaagg gaagaacttg aactaattgg 1920

1980

aagaaagaaa gaaacgaaga t 2001

<210> 54

<211> 2001

<212> DNA

<213> Glycine max

<400> 54

tcactagggt agtagttgct taaagacaca cggaaacatt taaggataat tgtggagaga 60

gagtgttgaa atttcaatca tctctctctc taccacggta tacttcaata aaaaaaatta 120

180

tttatgttaa tacctcaaac atacatttag aatcccacaa aattagtttg gtatcatgtt 240

tggattttca aaagttaatt ttgtaacgta cgtttgggat aaacctaggt ttgggtttgg 300

atcagaccaa caaatttgta gcttttaaag tgaaattact ttttatttgt atatttagga 360

tagaaacatg catgaagtaa aaatactttt gattagtata tatccaaaca aatatcaaca 420

cttttgaaca aaaagggtaa tcatgttcta ttaatatttt ttttttttgt gataaaagaag 480

tagctgaagc ttcttatttg acaattaaat aattcttctc ctatcggatt ggtccatttt 540

taaagtaaaa tgatacctca aattgtgact atatacacaa gccgtgaatc aaatcttaga 600

660

ctcatttttt acaacatact tttgattcaa acttatgttc gcaaacttta tacaaatatg 720

cacttagtgt gtttggatat ttcctagaat gaattttgag tgccaaatca aggtggaaat 780

attgcaaagg ttaaagtaaa tatttgttac tttgaccttt tcaagttacc atgttattga 840

taaaaggtat ttatcgattt ttattaatga ttaatctttg tcaaaaaatt tagttagtta 900

960

caccttcatt aaaaggatta agtctgtatt actcagattg ttactttgcc tgctttaagt 1020

1080

catttaaaat taattggctt 1140

ataactttta gcaagaaaaa atatttaaac acagaccaca ttatctcaaa ataaatttta 1200

attaaaatta ttttattcaa aaatcaattt ttatcaatat tcacccaaac acacaaactt 1260

1320

ccatacacaa aatcataacg taagaaattt caattttcaa ccatctcatg tgatctatgc 1380

1440

gctcttaaca cacatgggtc cctccatagt cataataaaa ataatatcat catcactgtg 1500

tggccataaa gagctacaag acacacatgg acacagtagt acactgccca accaatcaca 1560

cgtcgacagc acaacgatcc caaactactt cacctttccc aaaccagaaa ccaaaaccac 1620

ttgtcaccaa accccagtcc cagatagttt tctcaccatt tccgtatttt acttcacctt 1680

tagtgggtag tggttacatg atatcagcac actttcactc actgtgcttt cccgtgacac 1740

gcatctatac ttgtccagaa gaagtaagat ccatgaacta acattcccac acgctcgctt 1800

1860

tttgggaatt gggtttggtt tggtttttct tggttgtaaa aggtaaagaa gaactaattg 1920

gttaagttac tatatatcta gctagggtat ataatattat ataattattg ttattaaagg 1980

aaacaaagaa agaactaaga t 2001

<210> 55

<211> 2001

<212> DNA

<213> Glycine max

<400> 55

acaaaagatc acaaaattga acaataatga caatacctcg cattgaagta gttcaataca 60

tattctgagt atgactcgcg agtacatgta tctcatgttt cagtttttaa ccacagctgg 120

atgccatgga cacttttaat tctattcttt taaatgagat acctggccta tcaattttaa 180

gggtaaatta aaactcagaa atcaacatac taaaaaattg catgcatgtc cgcaaaagaa 240

aatgtttaag gcagtatata ttcactagtg cacaaagttt tgaccttaaa tattgaaata 300

gtgaccatac gagtgtaatg tttcagatat atataatgct aattatggac taattaatta 360

aaatagaggga ttgctttcaa taggaaaata gtagtgtaat ggaaaccggga aagtgaacta 420

gaaagaaatg ttaaaaatgg tctaggggaaa aagttaagga gaaaatggat tatacataat 480

gtcaaaaatt ttataccgct gattataatt tatatattat gtaggaaaaa tttattaaat 540

600

tcaatttata aatattaaac ttaaaagata aacctaaaaa atactatttt tctttatcat 660

aatattagta taaaataatt ctactaaaaa aaataattaa tatttataaa aatacatata 720

780

aactttggat tacttaatta aaaaatgatt cctaaaaaag accatttgaa tattatttgg 840

tggacgaagg gatggaccag agttcaatac tcgtgggttt tggcggcgtc aatgagagga 900

tgcacgtaat gttcgggtcc gtacggcatt gccactgttt aaaccatcgg acacttgaaa 960

gctcaaagca gtgactgtga tgagcagctc gcaatagaga atccaaaatt cccaaaagca 1020

1080

caacatggta actgaccacc caatccgggc tcgacagcgc aacgttagca aaaccgccct 1140

ggttttttcc caaaccagaa accactttat cgccaaaccc caatgtgatt agagtgttgc 1200

taggtgcacc tactgttatt gctggtgcac ccaaccttct ttgataatga taaaattatc 1260

cgtcttcttt ttccctttac ggatcaagtt gatccgcaag ttgattttta agacttacag 1320

atcaagttaa tcggtaagtc ttttatggat caacttgatc cgtaagtctt aaaaattaat 1380

ttaccaatca acttgatccg taagaagctt gcagatcaag ttgatccata aaagacttaa 1440

aaatcaattt gcggatcaac ttgatccgta agaagcttac gaatcaagtt gatccgtaaa 1500

agacttacgg atcaacttga tccgtaagtc ttaacaatct acttgcggat caacttgatc 1560

cgtaagaaac ttgcagatca tctatatcaa ctgtagatca actaaaacat atgcggatcg 1620

actcattaca tatgtggacc aacctgaatg agctaggtgc acaaaaatat ttttaaaaat 1680

acatagggat atttttgtct tttcatgtta agtgttaggt gcccaacaac aataatgctg 1740

ggtgcactta gtaacaccca tgtgattagg gatatataac aacatagcat actcacacta 1800

ctactcactg cggttttttt ctcctccaga agaagaaaga tccttaaatc aacattctct 1860

ctccctccct tggttataat agcaagcttt tcaagcatat ttccggggtt tgttcccttt 1920

tccttgttta gggttttctt tggaaaaaaa aggtaattag gtataataat ataattaaag 1980

caaaaaaaaaaaaaataaca t 2001

<210> 56

<211> 1162

<212> DNA

<213> Glycine max

<400> 56

agcgcaccgg gaaccaaaac gcgtgtacgc gtaccacgta tcccaattcc ataagaaatt 60

ctaaaaaccc acacgaacca ttttgggcag gaacaaaatc ccccagacga cgacacctca 120

gaaagttagg ggcattttgg tactttctgc atgtgttaga gccttgtttg tataaaggat 180

atagatattt gtctaaggtg tatattgtaa acaaaatatg actttcctat tcttacatgg 240

tcttttgtct tataaaagat gatagtgacc gagttgataa ttgttcacaa taataatatg 300

tgtaaagtga tagtcagtta acaagtgtcg actacccgtc gattccgtac aagatagtaa 360

tcatacgagt gtaaagtttc ctatagagag agagacatga atactaaggg ggattattta 420

attaaaatag aggattgttt taaataggta aataataata ataataataa taataataat 480

aataataata ataataataa taataagcaa aggagaaaga gatgttaaaa atggtggaag 540

gaaaaagata accctaaaaa ggaacatttg aatatttggt ggacgaaggg atggaccaga 600

gttcaatact cgtgggtcgt ggcgccgtca acgtaaggat gcacgtaatg ttctggttca 660

tatacgacat tgacactgtt taaaccatca gacacttgaa agcaaagcag tgagtgtgac 720

tgtgagagag cagcaataga gaatccaaaa tcccgaaaat caaaaacaaa acccaaaaag 780

caaaagagat atgattgtgt ggctgttagt taccacgaca acaacatggt aactgacaag 840

ccaatcaggg atcgacagcg caacgttaag aaaactaccc cggtttttcg taaaccagaa 900

accacttgtc gccaaaccc aatgtgatta gtgatatata ataacatagc atactcacac 960

taccactcac tgcggttttt ctcctccaga agaagaaaga tccgtaaatt aacattctct 1020

ccctcccttc gctataatag caagcttttc aagcatattt ctggggtttg gtcccttttc 1080

cttgtttggg gttttcttgg gaaaaaagaa gtaattaggt ataataatag tataactaaa 1140

gcaaaaaaaa agacagtaat at 1162

<210> 57

<211> 2001

<212> DNA

<213> Solanum lycopersicum

<400> 57

atctttcaaa gagacggatt tagagcgagt tctaatctaa taaaaaatta ttcaataaca 60

ccatacttga aattaataac gtttacaatt actttttttt gaaacgtagg ggcatgtgaa 120

attaaaatta aagcgtaaat gaaaattcac aatgtctaaa ttcggaacta acagatgcta 180

tgcaggagag caacaaaaga agaaacccta attgctaaat ggaaaaaagt gcaaggaaaa 240

atcaagtttc tatatgtgtc ttttaagtca

cggggataat gcacaaatac ctctcaactt atgcccgaaa tctcaaagac acacttatac 360

tatactaagg tcctattatt atttttgtta ataatttttt atcccttttc tgcttacgtg 420

gcactaaatt gtgggaccaa cgttgattgg ctttttttttc caagctagtg tcacgtaggc 480

cgaaaagggg cagaaaatta ttaataaaaa taagtttagg ggtaatagga ctttagtata 540

atataaatat gtctctggga tttcgggcat aagttgaggg atacttgtgc actatatgcg 600

660

tttagtgaat gtttgatttg ttgcattaaa aataatatgt atggcataat ttttaagaat 720

aatttatttc ttcgattttc ttcgtaaatg tgaaagtgat aatataaaaa tagcttaaga 780

ggaaaatttt gtttaactat ttttccagtt atctcatcat taattcatta ttactcgact 840

caagacaata ataactcaaa attatagggt cctttggtgc gagggatcaa aaataacgat 900

aatgtgataa aaaaaaaaat cttgtttagt tgtcatgttt agaataactt atccaccatt 960

tacattatag tgatgagata tgttattcgt aaataactac cttcaaaata acctatcctg 1020

taataactta ttcccaacga tagtaataaa aatttatgtt acgattattt ttctttatcc 1080

atcactggac taaacgtgtt ggaagaactt taatttagag ttggtgggct aactgtcgtt 1140

attgggaaac ctgtatgcat attgaaaaag taaaaaaaag acaaagagtg ttgtttgtag 1200

gtacaattgg tgtcgaatag taattggaga gtactgctgt gaagtgactc tagttgttta 1260

actgtttctt cactatgaac cacacgatga caaacgtagt acaatcgaag ccaaaaatag 1320

1380

gtatattagt actagcagtg agactgtttc gtttttgatc aaaaatttcg cgctcgagtt 1440

taatataggt tgtatcaatg gtgaagctat ctcacccttg atcagaaatt tcgagttttg 1500

1560

aaatttaatt gggggctcta atacgaaatt ccgcattggg tggaaaagga aaaagaaaaa 1620

gctatgatca atctactctc cactgttgca gtttaccctg ctatgttgta ctaaattgta 1680

ttcttgcatg aatctcaaag gataatattt gattttcttt taattataag tatttaaaat 1740

tatatccaaa aaaaaatata gcttatttgt gattccgaga tctgtgagta gaaaatccta 1800

aaaactattt cgagccaatt tttgagaact atgaaaatat ttaaagtaaa aaattatacc 1860

ttaattcaaa 1920

aattgatgac caagcactag attggtcata tagttttgag attataactt cttaattatt 1980

gggtattgtt aaactttcaa c 2001

<210> 58

<211> 2001

<212> DNA

<213> Solanum lycopersicum

<400> 58

60

gatctcgtcc cccgtccccc cccctcaccc ccgcctcctt ctttcttctt tttcggaaca 120

ccctcaccta tttcttcatt tcaaattatt cttacttatt cttaaaagtg atgaataaga 180

aaaaaatgaa aaacccaaca acttgttaca tatcgaaggg tttgttgatt atgatgaact 240

tgaaacattt tttaataaaa aaatttgtaa tttcttcatc tcaaattagt tttttaattt 300

360

ttagagtttg attttatttt tataatcttg gattaattca taattttctt ttctgttttt 420

atctttctaa aaacgtataa ataaaaataa ataaaaaaga attaaaaaaa gtcaaaattt 480

ttaattttca atgtggcact gacgtgacac taatgtgaca ataaaatgac gcgtgtgtaa 540

tacacttgct actgtgagac tggtattgta gtattagggt tgaaaaaaat cactttaaag 600

atgttaaggg ataatttaaa cgctagtgta gtttaagtgc taaagtaagt tttgcagcca 660

agttcgagag aacttttatg tattttctct ttaaatatat actcttctca ctgacgatta 720

tcgatgtaat gatctgactg ttctttaatt ttcttcaaag ttttatttgg attagtgatt 780

aaatggctaa gacttaattg tcaaagtaac tccctaacca tgatatttct tttattttct 840

ttttaaaatt gatctcttta ttggcttatt tctcctattt tatttcacct ctttcttccc 900

tatttcctct aagacgcaat aaaaagagtg ctttgaaaga aataaaagaa attgtatgct 960

taaaaaagta ttaggaagag aactattccc aatatttatg gacaagatag tatattgcta 1020

agatggcgga atttcaataa tttttaatga aatatataat gtattatga actaaattag 1080

1140

tcactagttc aagtaaaaat ttagagaaaa tggaaagaaa ataaacgaag aagagccaaa 1200

tctaattagg catatgctac aatggaaccg cattaacaaa caaaaatatt taaaactact 1260

cctaaaataa aggaggaaaa catatattat aaatcacaaa aaatattagt atcacaaaaa 1320

1380

tttaaattag ccaagtgaaa tatatacctt taattataat tttattttta ttaaaaaaac 1440

gatgtcatat atgaaatgta gagagtacta catagtgcag aagtactgaa tgtcctagga 1500

ccttagcata taaggagtat atactaaaaa taagaatgac gtaaaacacc atcaatgcaa 1560

ctattatacg aataaaaact caataagtat ggcccaacca ttacctgcca acccgttgaa 1620

atctcgatta atttaaattt acgtacaaaa taaataaaaa tttcaaacta aaactatcta 1680

atcacatttt gtctggtggt atgtagcata attttgtggc tcttaaccac cagatactag 1740

cagtgtgtac gttttatcca atcacaaatc gacaacgcaa caccccttaa tgcttaaacc 1800

actcttgtat caggttatta ttattccctt tcactcaatc aatcaaaaat caacaaataa 1860

aacctccccc cccaaccctc aatcatgctt cttcttccat atagtctctc aattagtcca 1920

aacaaacctc tctatctatg ctaggtagct tatatgattt aatagttcga ttaatttaaa 1980

tacgcattaa agatagagga t 2001

<210> 59

<211> 1812

<212> DNA

<213> Solanum lycopersicum

<400> 59

tttcgtcaaa agtgttgtca aatttctatt ggttcacaat actcttcaac tcatttaact 60

tttactcacc gtttcaaaaa caattaactc tagtctgact tgattgacta gacacggagt 120

180

ttgagatagt aataattaac aaaaacggac tcactctctc ccttttctca ttattactcg 240

gccgcaaact cttaaaaata cacgccttaa ttaatactct tgcctaatag agtattcatt 300

tagcacattt ttaagaaaat tgtaaattac taaggatatg attttttata taatggacaa 360

ttgttattaa aaagacgaag atatttttta aaacttttat tcgaaacaaa tatgagaaaa 420

ttggaaaaaa aattcagaaa atgttttttt cataccaaac acactcctaa gtttaagtag 480

ggaaaatcgt ctgatatacc tctcaacttt gtcatttagg gttgatatac cccttgttat 540

gaaagtggct catatatacc cctacttgta aacaaatggc tcacatatac cctttttctc 600

660

ataatcccat atgaataaat ttaatcctcg tcaaacatat ttttttttac tttattttgt 720

ttcaataact aatttataat tattattttg atgataaaat ttatttatgt ttcactaata 780

ttcttgtaaa acttattgta gatgatcaaa ttttttcttc gaatccgaaa ttaaattaca 840

atacacatac agaaaaatag tttaattttt tattctttaa actaagaaat gaaagaaaaa 900

aacaaaataa gataagaaat tcaaataatt ataataaaag aagtcaaaaa ataatttatg 960

1020

taattttttt ttaaaaatta gaagtaataa atataaattt 1080

1140

gtataacggt aagggcatag atgagccact tttataacga ggggtatata agctccaaat 1200

gtatttagca tatcatttaa 1260

1320

tttccttcat actaaacaca cttctaatct aagtatttag catatcattt aatagaataa 1380

attaaataaa tactttagat gaatattaat tggtatcttg atttagatag ataattggga 1440

1500

1560

atcaaaatta atctaactgt acgaaagatc atcaaaatat taattagtat catccaaaaa 1620

1680

tttgaataaa agaaaaagac tacgtattta atatctcata tgtaatatat aatgaataat 1740

1800

gactaatatg gc 1812

<210> 60

<211> 1880

<212> DNA

<213> Solanum lycopersicum

<400> 60

atctgaaata acttttgttg ggcaaaatat cccagatgtg gaagagagag aatatagtgg 60

tttttaaaaa accagagtgg tttcgtcaaa tgtgttgtcg atttttcatt ggacagagaa 120

gatggattat ttgttatgtg tctcaataaa acccacaagt ttttttttgt acagtagaca 180

atagccgtgc gcatcttgta gccaggtgat gcgaaacaat ggaataacgc ccaccatctt 240

tttgaaattt cggtgaaaat agtagcttcg tcacgaatta atatttgata aatattatta 300

tacattcata atcactgctt cttttaaata aaatattttt ttcgttttat atcaattgat 360

tttttttata tgtttattga aaaaaaatta taagtaagaa gatgatattg ctaattccac 420

acatattact tttagtatga tgataaacta atataaaact gagggagtag attatttgtt 480

ataataagat agagatactg ttaaaaaata tgatatagta aatgaagttg gtataataaa 540

aaatattaaa aggaaatgct tcactaatga ggccttatat agcacgattt ttttttatga 600

tagattatat atattatagt tagggaatta catggctttg agacatattg gcctctacat 660

agtttcgaag cctattggct tccacgtatt ttttatatat tagttattac ggctacctta 720

tcaaaacatg ctaatttaat acaaattatt cttaaaacga gcctagtaga tataactctc 780

tgttggtcat cttttacaag cgccatagta tgctcggagg ggtagatagt atagtaattt 840

gggaggacga tgttagcgtg gtacctagtc ttgacaaaaa ctctgcttca tggtatatat 900

aacttaagac tgatgtttttt atataattgg tatagtttaa tttatgattc cattacgttt 960

1020

gctattaaat atatttttta aaataaaaca aaagaaatta ataaggaagt atgtcttttt 1080

tattcggaca tgatgattca taaactggga taaagattta taatagattg tagggtaaat 1140

cttatattta ggtatttcat ttttcttataa tttttatcag ctgtatataa gtttcaatgg 1200

tttaatttat tgtagtaata ggttattgtt ggtgcttctg ttgaataaat aacaaaatca 1260

agcattatgg gtatatagtt gtttataatt tctttgatac gatggtacaa aattatctta 1320

1380

1440

tcaattattt ataaaaattt taaattctta attaatttaa ttcataatta ataaagataa 1500

aataataaat tactatatca actatcattt tttaataata tattattaa aaattgaata 1560

aatatttaaa aataaaaaaa tttatattta attgataata tatcaatcaa acgcaatata 1620

aatttaattc gcaaaaatca aagcgtaaag ttaaccaatt cattggctgg gtggatagca 1680

1740

attgtgtggt ccaactggat attcactctc ttatcccttt cgttttcctc ttttagtatt 1800

1860

gtaataatta 1880

<210> 61

<211> 2001

<212> DNA

<213> Zea mays

<400> 61

cccgccgagc aagtcaatcg ccccatcatg cggacttgct cggcaaatgg gctagagaga 60

ggtttatggg cctcgccttg ggtaccctgt tcccggtacc cgacaatgac cttcctcgga 120

180

ggctatctct agaacgttgc ctattcttat acccatattc aaactttatt gataaaatgt 240

tggacaggtc tggtgccctt ggaacaagtg ttgtttccat tctccagagt ggactacttc 300

ttgcgctgat ttgtttggtg agttaccgaa ggagatttag gggaagaaaa acagggcact 360

tataagtgat atattgttta tctcagatgt attgatcact tctctggtat tggtgcaatg 420

tattggggta ccgatcactg agtaatcacg caatgtattg gggtactaaa tcctctctgg 480

tatcgattat tcatgcaatg tattgacgat tttaataagt gaatcgccaa tgtatatgat 540

atttccactg gcggtgtact taatacagcc gccagtgtat atacatcatt tccactgacg 600

gttcagttaa gtgaaccgca agtgtatatg acatttccac tggcggtgta ctttatagaa 660

ccgccagtgt atatacatcc tttacactga cggttcagtt aagtgaacca ccagtgtata 720

tgatatttcc actggcggtg tacttaatac agctgccagt gtatatacat cctttatact 780

gacggttcag ttaagtgaac cgacagtgta tatgatattt ccactgccgg tgtacttaat 840

acagctgcca gtgtatatac atcctttata ctgacggttc agttaagtga accgccagta 900

tatattatat ttccactggc ggtttactta ataaaaccgc aagtgtaaat acatcattta 960

1020

agacgggccc gtctgttttt ttcactggcg tgctgtaact gaaaccgcca ttataaattt 1080

ctacgtgccg ccaccttaga gctcttttct actagtgtta acttcttttc ttgtagacca 1140

tttggaaaac aggaaacaac gcggtactgt attcaacaac agatggttgt ccacacctat 1200

1260

ccgtttcttt gttttttttc ttctgttgag ctccaacttt atgaaacgtc gtaagctggt 1320

aattatgaaa tgtaaggatt tggagagaga aaaaaaacgg gagggaaaac catgcatgct 1380

gctgacgcga cggccggacg cagacgcaac aatgcccccg gtgcggcgtt gtcgagcagc 1440

1500

atttatttat ttatttttct tctctccctc cctccctcag atttgttttc gttttcatta 1560

1620

tatatatatt cttttattaa ggtccagtaa taagcagcac agaagcgcag gtgcagcagc 1680

agcagcgtca gcgcccgagg cgcgcacgag agaaacagag gctgacgagg tggggcccgt 1740

gcgggccttg accaatcgga gttcgacaac agcctggcca cccacaaaca cacactcctt 1800

cgcctcgcgc cggccgtcgt cgcctccctc caccgaacga tccctcctcc tcctcctcct 1860

ccctcctcctc gcatcccacc ccaccccacc ttctccttaa agctacctgc ctacccggcg 1920

gttgccgccg ccgcaatcga tcgaccggaa gagaaagagc agctagctag ctagcagatc 1980

ggagcacggc aacaaggcga t 2001

<210> 62

<211> 1610

<212> DNA

<213> Zea mays

<400> 62

60

agtccattga tgcaataatt gacatataac agttgtacac attatcttgc taaaaccggga 120

180

gcaatatcca gattctcaag atctcttggg aaccaaacgc cgccaaaaat cggttccaca 240

300

tactctactc caggaatgac taggacaaca tagatagata gtgaagggaa aaatagtggc 360

tgcccttgcc aacatctttt ggttatgata gcaagtgtaa taaggtgatg tagatggact 420

acatgagacg ccacgtcaga tttaaaaatg tggaacaaag agaagtggag aaaaaaattg 480

ggttgctcgc tagtagccaa ctgagagtgg gttggacacg gactccaaag actccctgtt 540

agagagagag aggggttgac caaatattaa atatacaacc ggtatcctcg aagagtttatt 600

atacaaatga ttgtctatac gtgacatgac aacatcacaa accaactata gactacatat 660

tactctatct atactaaaat ataattcatt ttaacgctaa ttacatattt attaattaac 720

ttatgaatgt attttgtatg tatatctaca ttcattatct tctattcgaa tgtagataga 780

aaaagagcaa tagaactata ttttgggaca gaagaagtat taattagaga gtatttggtt 840

tctaaagact tagtccctca attttattct tattcagtcc ctaaattgac aaatacaaaa 900

actgaaatta agttttagat tttatatttg acaattttat actataataa aatagagaga 960

ttaaaaatta atacctagaa atcaaatata ctcttaaact tcacttcgtg ccagaggcta 1020

gctgcgacta gctgcgagat tggaatggat ccggtttcga ccgtacgagg aaggacgctg 1080

1140

ggcatcggct aatgggagtt cgacaacggc agcctcggaa cccatcccgg tttcgcgata 1200

ccccctcact gctccagtgc tccctcctcc cctcccctcc gatcaagtcg gggcaacgcg 1260

catcactcgc tttaaatccg cacctcccgg ccggtcccct tatcacctca ccttctcctt 1320

tgagtcctct ctctccgccg ccgcagctag ctgtgacgtt atgctctcgc cggcgccata 1380

gcgccagcgc ctaccgtcta caactatcca gccttaggct tacctatccc gtcaatcaag 1440

cctctcgtaa ggaacaagga aggtagctag ctagttctat agctgctgtc gtcgtcgtca 1500

tcggcggcgg cggcgcctgt tcttagagga taaggttgtc ctagcggaga gggagctagc 1560

1610

<210> 63

<211> 2001

<212> DNA

<213> Zea mays

<400> 63

cccccctcta ggcgactatc aaggatccct atccaaacgg ccctaagtgc gagacgggta 60

tggactagag gtcggtgccg aggaggtagc agggaggtgc gagattctgt tacagagatc 120

gcggggtgct cgggccacac aacggcagcg cgaggcgttt ggcatggtcc gccaaactcg 180

cgaagacgac gaccgggagg cgtggggccc actgcgcagc gtgatagttt ggtcgcgcag 240

cgggtgcggt gagcggctga cgagagggtc ccgcccgcca gcgagccggg gtgtgcgatg 300

ggccagcgag cgcgagaacg tagttgggcc gcgcgaagcg aggagttggc tcgaaattgg 360

gccgaataga gtggaattgg cccatgaagc cttttttattc ttttcttttt atattttctg 420

ttttctgttt tcttttcttt ttattttttt caattcccaa tttgaacttc aaaatatgag 480

tatgaaacta gtacccaatt tgaagttcaa gtatgacttt gaatttgtac tcaaattaga 540

600

tttatccctt tctttctttt tttcctcaaa tactcttctc attatcattt ttatttaatt 660

catattatta ttgattttaa tgcacaaaca aaaaatccaa tatgatgcaa tggtttattt 720

gtgtcttatt aaggatctac ttttttttac atgagtggtc acatgtaatg ataactagag 780

gtacacatac atatataaag gaaacaattt ctccttttat tcttccttac aaagtgggta 840

ttacaatcct ttcatttgca ctttcttcga tgcattgatt gttgattatc ccatgtttct 900

atacctctta gaggttggac attgctcctt tatataaaaa cacacctaga acgagtgacc 960

ttagagttgt tctccatctt tggatgctga ataagttgca tgttagaagc tcattagccg 1020

1080

ttgattgtca cagtttgtgt cccatgtaat tctaactcta gccatggact tttttcttcg 1140

gtctattagc ttgagtgcta tgttaggttg ctaccaagac aaaatacact cacttgtgag 1200

1260

tttggggatt gagtgaaaaa tgagctattt tcctactcaa tcttcgagaa ttatagaggg 1320

aatttaagtt tttaaactag ccattgtcta aaaaatgtca tcttcttccc cgtgtctaac 1380

tcggccatgt cgctcgtgct aggggaggtt cgactgttgt aacacccaaa atttctattt 1440

tgagaatcaa ttaaatttct ttaaaaatat ttgaattaat tcttttatgt gtattatact 1500

gctaaaaata tcttcttctt ctcggccgta caagtaggtc gctttccaat atatattctc 1560

gttttacaat attttcaacg cattttttcc ttttattaag gtccaagtaa taagtagtag 1620

taccggtggg gaaagagaaa gagagagaag cgcggccgca gcgagcagcg tcgtcagcgg 1680

ttggggcgcg gacgagagtg agaggctgac gaggtgggcc cgtgcgagcc ttgaccaatc 1740

ggagttcgac agcagcctgc ccccaaaccc acgcacgctc atcacacaga cacacacact 1800

ctctgtgtct ctcgcgtcgc gcccggccgt cgcctcccct ccctccctgc accgaccgat 1860

ccctcccctg aacccctcct cacatcctac cacctcttta aagctaccca gctacctgcc 1920

tgcctcgcct caacctcgcc ggcggccgca gccgcataga ccggaagaga aaggaagcag 1980

agatcgggagg caggcgaaga t 2001

<210> 64

<211> 1589

<212> DNA

<213> Zea mays

<400> 64

ggtttcgctc gtcccgctgt cgtgttttga ttggacgcgg gggttttgga gcggcgtgga 60

ggcagctaga ccgtcggatc atgacgggga tcgttattgc gggccagaag gatgggccta 120

gcgaggccgg cgttcgagtg atgcgctcgt cagggcgcgc ggggcaggga ttggtcgagc 180

ctggcgtggg ccggaagggt gcggttcagt ggtgagatat ggttttttcc attttgcttg 240

ttccagttcc agtccagcct tccagggtcg gtcgggcaca agggagcgag atggcaagtc 300

360

gtctgtgagg ctattatcaa taaaagtttt atgggagttt catgcacatt taatggtatg 420

480

aattagtttg atgtggatga aatcatgtca acccgttttc aaagtcttgg aaactatgtg 540

aaatgtccat tgatagtggt ttgtttaatc ttaccatatt ttactaacat ctattggtta 600

660

tattatttca tttttagttt cataacactt ttgatgatgt ggcattgttg gaaagagcga 720

tatgaaatcc ctattgtgaa cagcctgagt gtcctcccct gtttcttttt ccctcggaga 780

aactgtgatt gagggttttg aataacaagg aattttagcc cactcaatac cgtttaattc 840

tctttgtcca aacaaatcat tattgtagat tattcattat tattaggaaa gattcaggtt 900

accctccata cctattaggc tttatttggga tactttgtat agtgttttat atattataat 960

ttgtggcttc tgtacagtgt tatatcatca ctagatgtgt ggtttctgta ttgtcaccaa 1020

ataacatggg ggtcatgtgt ggtttttgta gaataatttt atagtacgat gttgtcatga 1080

1140

tgtcatgaat gtacaatgtt atagaaatac aagaagtgta gccttgcata tttgtataaa 1200

ttgtttgggg gaaagaatct atgcttcaaa aaatgtggcc ttgtagcttg cacaaagtac 1260

atgttgtaca atgctacaga agaaacatgt cttgccgctc agccaaaagg accgaccgtg 1320

agaatcagag cactgcccct gagtctaagt aaacagaaaa agaggacaaa atgagttatt 1380

acaatacaga gaggcaaaga ttcttggaac tatagaccac aaactttagg gtcgtcaacc 1440

ccaccaccca cccgtgcccc tcgcacgcgg gcccgcctcc ttgcgcacta ccaccggacc 1500

atggtccctt ttaaatactt caccgccacc cactccccct ttttgcgatg acctcattgt 1560

catctttccg gtaaactagg cacctcttt 1589

<210> 65

<211> 21

<212> DNA

<213> Glycine max

<400> 65

tgtaggtaac cctcatagga c 21

<210> 66

<211> 21

<212> RNA

<213> Glycine max

<400> 66

uguagguaac ccucauagga c 21

<210> 67

<211> 328

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence

<400> 67

ggcagagccg tgcccgtctc atcccctgcc cgtgcaagca gctaggtagg acgatttgag 60

cgtggtgtta ggccgaaccg ctgaaggaag attgctccac tgttgactgc attgtaggta 120

accctcatag gacaaatgta ttgcttatat tcagcaatat aatgttcgtc ctatgatttt 180

tacctacaat atagtcgata gtggaagaac ggtaacatat gtggtttgca gcaggtgagc 240

aggatgggtg tggatgattg aatatctctg ttcagtgttt tcatcatctg actgaacact 300

gaatcagctt gctgacgtta gaggttag 328

<210> 68

<211> 21

<212> RNA

<213> Glycine max

<400> 68

guccuaugag gguuaccuac a 21

<210> 69

<211> 21

<212> DNA

<213> Glycine max

<400> 69

gtcctatgag ggttacctac a 21

<210> 70

<211> 3058

<212> DNA

<213> Solanum lycopersicum

<400> 70

aatcataaac acaaatataa gaaaaaattt tgtgatagat taataattaa cgtaaaactc 60

atcaatttac aaaaatctct tagaataatc agtgaacgat tcatataatc gaccctaact 120

gctcctaatt ggtgtgttat cattgttata agctaaaagt agttatgatg aactatacat 180

tatgtggtaa acaaattact tcaaaccata atatattgtg gggcttgatg ggattttgtc 240

aatttcatca aagaattttg ttttgggcca ctttgacatt atgcatccaa aaaggtccaa 300

tagataacta gcccacttaa taagttcatt tactcctctt gctgattttg gactaaacag 360

aagagccaga aatcacagac ttatccattt tgtggtccag actgtgtcac cacctttgag 420

aaaaggtaac aacgacgaca tattcagtga agttgcacga gtagagtttt aaaaggagag 480

cgtgtacatt gtcttacgaa aaaaatttgt gttacatagc gcgtcatttg aatgagacct 540

aatgaggtac aaattcaaaa taatcagact ctaatataga aaacatgata aatacttgat 600

660

tatcacataa actgaaacgt cggaatgaat aaaaaatgtt gttatacta ggtttcttgt 720

gactaataat ctaaagctcc tttgaagaag taattcctag aaattaggtt tacctaccac 780

tactttttttc ttttttttttg acacaagtga aggtataagt tgaaacaatg accaattact 840

catgggaagt tgtttattag cccacaggtc tcattggcca cttatatttt agcatagtta 900

cttcgagaga tcaatcaaaa aacttacaaa aatatttatt cagtagagtc gatagtaaat 960

atttatcatt caccatcgta tgatatacat ttgatatata ttttaggtgt tgattttctc 1020

atacatacaa gttgtataat ataacataca cttcagatac atatagtttg aagtcaaaat 1080

ttatatgcaa aatatgaggt atgttaacac gtgatctctc cataaagcta aataatttat 1140

gtatatattt tttaaatata catataatat tatattctaa tttgttttgc ccttttttag 1200

tggtacaatt atatttttaa aattttaaat tcgtctctag ctaacttgca catatagttt 1260

gaagtcacat acacttggta ctctcttcac ctacattcaa cttctgcttt gaaaacatac 1320

atacatagac ataatatatt cgtataattt taataaaaaa atatcgtata cacgcaaata 1380

tattatttta tttttaaaga aaatgaaaat ccaaaatatg acaatcccaa gtttgtggcc 1440

aggtgttaaa cacttgtgga ctaaacatat atatttattt tttcaattta tccatagggc 1500

acacacttag aaaaaatggg catttcccca tagttttggc ctaaagttcc taaaaaccac 1560

tggcttcttc tgcattctcc aaaactgtgg aaccaaaaga cttataagta ctgtttcttg 1620

1680

attaaaatta tgtatcgtct tttaaatcat ataattatat tgaaagttga aattaaaaaa 1740

cttactaaat ataaataaaa atgcttaaat taaaacagaa ggagtaatat ctaaaatgag 1800

ttatatatat atatataaaa gctttattta atacgaatat tttattcata tagtgtgagc 1860

tcataaaatt tattcataat ttgagaatag ttaatgactt atttttcgat ataatattgt 1920

tattctcttc tcaaaagatt tactagtaat gcgaggactg ttaatgacgt attctttaag 1980

2040

agatattcga aatggtaagt atcaaatata tattttgttg cataaagttt ttttctaaac 2100

taaagtgcag agaactttat acactatagg agtaatattt actacttata taagtagtac 2160

aatattgagt aatatttact gtttattaat tattatatta tattaatata ctccctatcc 2220

aagaataact cgactttacc aataaaaagt cactgtaatt tctctttgct cctgagttaa 2280

atatttttat tcggttattc tcgtagccga ttaaagttat atagatatta agaaaatata 2340

tttaggcttt ttatattata tttaaaagtt aattatgttt attttagtta tttatcaaat 2400

attatcttat accctgcgta taaactaaag taaatatcac attaaattta cgtatagtaa 2460

aagtaatgtg attattaatt atataaattt tattaaatat gtgaataatt tagctcctga 2520

tctactatca aacgaattat tatagtatta gtgacggagc catcatattt ttaagggttt 2580

2640

atatatacaa taaatgtcaa accccattca atcattttaa gtatttattt cttcaaatta 2700

tcgaactctt ttatcgaaaa ttttgaatcg accgctatat actaatattt tatttaaaac 2760

cacagattta tcattcatga accgacaaat gtatttaaat ttactgacca tataacagaa 2820

2880

accaaaaaaa atataaaaat aatattactc acgtactgag aattaaataa taaataaata 2940

aataaagtga taaacaaaaa caagtgactg gaacattgga aattatccaa aaccatcaaa 3000

3058

<210> 71

<211> 2245

<212> DNA

<213> Solanum lycopersicum

<400> 71

tcttgtcaat atgatctcaa ataacaaata attctatgac aatatgtatt aaccatttta 60

120

180

ttaaataact ttaaagtaac tttcaattaa tattagtttg aattaaaaaa aataatgaat 240

tagtcatgag atttgcgtac tcttactttt gaagagtgtg ttatcactct tataataggt 300

aaaagggaaa agggtctgat atacccctca actttgtcat ttggagctga tatacccctc 360

gttataaaag tggctcatat atgcccttac cgctatataa acggctcaca tatacccctg 420

480

atttatattt attacttcta attttttaaa aaaaattatt taggggtata tatgattctt 540

aaattatttt ttgacttctt 600

660

taaagaataa aaaattaaac tatttttttg tgtgtattgt aatttaattt cgtattcgaa 720

gaaaaaattt ggtcatctat aataagtttt acaagaatat tagtgaaaca taaataaatt 780

tgattatcaa aataataatt ataaattaat cattgaaaca aaaaaagtca aaaaaatatg 840

tttgacgagg attaaattac tcatatggga ttatattttt tagaaaaatt attatttttc 900

atttccgtta gaggaaaatg gtatatgtga gtcatttgtt tacaagtagg ggtatatatg 960

agtcactttc ataacaaggg gtatatcagc tctaaatgac aaagttgagg ggtatatcag 1020

acccttttcc ctatgtaaaa tctaaaatta agtttgaact cacataaaat tatataggcc 1080

gtaacctact taacccattg tcatcactaa ttatatagag atcttgattt gatatattaa 1140

aagtaaatgc atcacatatt ttttttaaaa attatttatt tgtaaaaata aggtcgacaa 1200

atactgtgaa atcaatgtga aaaaggtttt gatcgttgag tgtatcttta actatggtga 1260

1320

aataagatat ataactaata tccatatatt atattacata tataaattaa aaatatataa 1380

cgaacaaaaa tactacaaaa ttaatggaat acactctatg aatccagcat acacaaatag 1440

agcctctacg taagccagtt gttatctaat ctgtttagtc actatgacat cagataagat 1500

atctgtttag ggtacccgtt tacacatcag cttttaaggt ttcctactta ccaaacactt 1560

ctccttcacc aaattgtttc tccaatctca ttcaaacaaa cacacaacta ggtggttcga 1620

acaaactcaa taattttttt ttaaaaaaaa tttatattta tactaaaaat taaataaata 1680

tacatgtaca tttgtttctt aactcataac aaaattaatt aacgactcag tgggatcgtg 1740

aaaatgtttt ttacggctaa agttatgaat ttaaaccata gtatccacaa aaagttaagt 1800

ttccattttt tttcccaaag aaaatttaaa ataacaaact cttaatttca aaatatggtg 1860

cacaaaatat aaggggaaag ggttaaattg agataatccg gtgtcatttt aaagcgaaca 1920

1980

2040 aacaaaagtg tgtaacgtga caatgtgata taatacgaaa taccctagga

acataaaatt cactataaaa ggacaatgtc ataattaatt tttgtaaata aaatattcac 2100

2160

2220

taaaaagatt gaaacaaaga 2245

<210> 72

<211> 2234

<212> DNA

<213> Solanum lycopersicum

<400> 72

tatgatcttt ttcaatgaag tgattcagtt caatggaact caaattttat atgtggtagg 60

120

agaattgaat acataaaagg tagatctggt acataaacta 180

ttgtgcttat gtagaatttg gggaagaaac gtcaaggaat ataatgtaaa gtatagtaga 240

caatttattt tatcgtatac atattaataa ttatttttcac gattcgaata tatataaccg 300

atagatcaca caataataaa tattagtgtt gctcatcgaa aactccgatg cactaatgtt 360

tgccactaat tcttaagata gataacaaac acatctaaac attattaatt aagtgtatat 420

atacaacata ttttaaactt attctataac tggatttcaa tttaaaaaaa ataatgatgt 480

gtcatgtccc aaagttagtt gcactctaaa aaaagttaaa aggtttttaa ccaaaaataa 540

600

ttttaaacaa gttttacacc ataatgtagc aatccatcct gttagtgata ttgtctgctt 660

taaatctagg aatgtacgtc tttaaaatgc gtcattagtg ggtaagacat gcttacttaa 720

aacacgtcat taatgaataa gatttgttta cttatatact caacatctct catatatttt 780

actgatgtga aattagttat cttaaaccgg aatgtcagta cacttcattt gtatcttttt 840

ttatgagc cattatcatt tacatgtaaa agtgcacctt aaagctggtt aagcttataa 900

actataaatt gttcattttt tctcgtttaa taatcaatat ctacttaaca aggcctgttt 960

aatagatgat aatagtttaa gtagaaaaat gaaattgtaa cttttttacg acttttaaca 1020

tttcaactat cagttagtaa tatgctcatc cattacatat tttaaagaga acaaagaacc 1080

attaaaaggt taaaaactta ttataaagtt aaatattttt tcagtatata tgaaaggacc 1140

ttacaagtta caactaaatc ttttgaaaga aaagtatcgg tcactactaa gttttccaag 1200

1260

1320

aatatacgga caaaaaaaaa taagaaaatt tatcaacgta tacataagaa aagttgcata 1380

cttccaaata gacatgatac ataaacatga tctttaactt gacgtcagtt ggcaactata 1440

tgtgcacaag taggcactta aacttgtata agattgaaca attgacacat tcatcctaca 1500

ggcaccctac atgaaaattt tgtgtcctgc gtggcgtcct acgtgtatca tgtcatgcat 1560

gacatgtgtg gctacttgtt caattttata caagagtaag tgcctacttg tgcgcatcca 1620

aagttgaggg tcatagttac cgactgacgt caagttaaga gtcatgttta tgtattatgc 1680

cctccaggta acatagattt gaagaagcat ggaatgcatg tagatcttac ttctcgtgaa 1740

aatggttttc aaatacgaat agattagtct cggctcaagt aaaacatttt aaaagtaagt 1800

acttaagaca aaataataca ataataaaaa agttatgata atattaaata ataaaaacta 1860

tagcaaaata taatatatta tcgaagcaaa cataaatgtc taattcaagc ctcgataaat 1920

gaaaaaaata atctaatttg aacaccgcaa ctttctttta aatgggccct ccacgacact 1980

tcatgatata tagtaaaaca aaaaagtctt tattctcttt tcttcttgac tagggaacca 2040

ttagatttta aagacattaa atctattacc cttaccctaa gaataagaag atgtaaagta 2100

gaagagaaaa caaccaaaac catatatata catatatata attacattat attgtcttat 2160

2220

tttcttcata caat 2234

<210> 73

<211> 1302

<212> DNA

<213> Arabidopsis thaliana

<400> 73

actatatacc ctccaattat tgtgtaccaa agaccctcat aggatttatt caaatattgc 60

aaaattgaaa gaaaaaatgt taattaccct atccatgctt agatattttc aaacctaaaa 120

atttggtgca tttttgtgtt tatacataat tagtaaagga gagaaattag agttccactc 180

240

agagaaaaat gtgattctgc caagaaagtt tgatgctttt ttacttagtt catcgaatta 300

tagagattaa taattgaaaa tcctacaaaa aaccctaaca tttaaaacca tttttattta 360

atacgttgag agagacagag atattaaaat cgtatgattc gtaagagtgt gatgttgaaa 420

atgagagatt aataatggaa ggtggagatg gcgcgtgtgc gatgagtgat tgcaacccta 480

aacagaaaat agtagggcaa tgaatgagtt tggacacgtg tcgacatctc gatggtaggg 540

600

ttacttataa aatcgagttc agggaatcat gtgacctaac cttaactagg tgaagcctcg 660

tacgattttc ccaacttacg gaactaccct tgtgattggt taaaaatgta aagtaaaaca 720

ctactcgttt gtgatgtttt catgatatct aaaactctta ctgctagttt tgataaatct 780

tactgctaga tttgcttttc cttttccaat ctatagtact gctaagatta tttttgttag 840

tgaaattaac agaaatgtct tttgtattag tctactttat aaaccaccat tttgatgtgg 900

ccaaaaaaaa aaccatgttg ataaattcag tttggtaaga gagtctgatg agacacaaaa 960

aaaattatgg gaaagaaaga aaacataaat ggataaaata aacagagatt tgaatataag 1020

atatagttta ttttggttat cttagggttc gtccgatgtt agagatcatg tctgaccgaa 1080

1140

ggacacaaag gtatgattca attagaaaaa ctaaacatag cattattatt ctgtttggtt 1200

atatttaaac ccattagctt cttttctctt ttgatttgca cattgaatcg cttattaatc 1260

tcacttggtc atggtctcat agaagcattt taagcaaatt tg 1302

<210> 74

<211> 2001

<212> DNA

<213> Arabidopsis thaliana

<400> 74

aataaaaaac tactagatta cagctcttga ttggttgata acttgatatc atatatcgtg 60

tttgtagttg aagatgtaaa gatttatcat cttgtatttg aattttgcac aatatataat 120

gtgatgattc tatgtttgtt tgtaaactta tgtgcaaatg ggcgataact aaacgttcgt 180

240

atatatgaaa cgattcattg attttaagta tttaatttat tttttaagat tttagttgtc 300

ttaacctcgt acgaagaaga aggatatata ctatttaact cgtggagatg atgaggttcg 360

gatatttttc cttaagacca caagacactg acacgccgac tgatcgataa aaaaagttaa 420

ggcctacaat acaaaagagt agcagattcc cttacacgtg tttcatttat gatctgttaa 480

ttaactccat tgagcttcaa tatctcatta gccgaaaatt aacttgacgt tttccattga 540

actgtcgaaa aaatttgtag aaaaagtgaa atgtccccta tttaacttcc aagtttaaaa 600

cctaaaatta attagttacc gttttgatga ataacgttga aagaatcgtc aacatatcaa 660

atgatgcaga tacaacaagg gacaaaaaca tcaaaaaatt gattaaaaat aatcattcag 720

atattagcaa ttaaactgta agtaaatgat tgcatatata tatatgaatg aatacatata 780

tatgtatcaa actgtaagta aataatacat acatatatat atatatatat aggaaaatat 840

ctcatttttc aatcattttc agaggtaaac atttttaaag acaatttttac ctaacccaac 900

atcttactat atttaaccag ctctgcgaga gcttcaaaac aatgatgaat tttctgcccc 960

ttttcattga ccaaataaaa gttcacaaat ttgtaaaacc aatcagcatt aatttcgcac 1020

gtagtttact tcgtccgatt ttagatcaag tagacatttt aaccaaacat attgaatcac 1080

ggttcaatca agatacatgg ttgcgagtat aatatatcaa tccttcaccg cattagaaaa 1140

ccaaattttc tatttaattt agcgtgttct tttaatgaac gcagcgtcag cgacaacaaa 1200

gaagaaaaac agatctgcgg cgagaaaaaa tacattgatt agagaagaac tgccgcaaaa 1260

gatgtacgat gatgaagatt gacgtgacgc tgcttaattt cagcgattca tttgatgcaa 1320

ttgcaattca tcggctatca tcggcgactc atccgaaatt gttatttttca agctttaatg 1380

tcgttttact tacctactac ttctcatgac acgtcgtttc agtccgctat tgccgctggc 1440

gcagcatcaa ataaaagaac acgctaggat agcttgaaaa gggtacaata actagttata 1500

1560

1620

taaaaggaat tttcaatggt ccgactgtta caagtagatt tccattttta ggaatctagc 1680

tataacgatg aagacagagt tatcattttg ttccatcaaa tatatgacga tatttatatt 1740

taatttcttc aacaacaaat atctcatttg ttggtgccac tttcacatac ttaggacgac 1800

taataattaa ttaaaaagga tttatgtaat aacgcaccat attaacctgt aacaagaaga 1860

1920

ccttttatct attcttgaaa aaaagttacc aattcttgag aagaagaaga aatcagaatc 1980

aagagaagga gagagaaaga t 2001

<210> 75

<211> 1597

<212> DNA

<213> Arabidopsis thaliana

<400> 75

60

gtaaaaaaca ttctcacacc ttctctccca agtgcgtgcc acgcagacac cattatttgc 120

180 catgagtagt tagttgctag ttgctactaa ttaagaaaaa

aacgttgtct agttataa tccatgtcca tatacatcta gatatatgac ttagatacca 240

atgtttcgac tcttttaggt ctacacgtac gttgcccaaa cttcatattt gagacatctt 300

tatttatttg tcaacagaaa tattgaaaca tgcatggatc ttgatattct aaaggtgcct 360

gaattaaaat agaacgataa cgttttaaaa cctaattccc aatttgtagt ttatatattt 420

cttgtttgat ccatattatt acaagcctat atatagtgta tataaaactc aattaagctt 480

caaaaagtca gaaaactcat caatagctca agttcacta attaatgccc gaatttaaag 540

ttcatctcta tagtttaaaa tttgttcgta gctagatttt gcatcttgga gtggttagtt 600

atgtttatta gacacttgga atgcttttga atgtacaatg tccggaaaaa aaaactaata 660

ctgaaaaggg gatttgtcca agtggtggta gataggagga ggcgcgtgag attggggaaa 720

cattggtttc ttctgcaatg aatgaaacga aagcagctat catcattttc tttttcttac 780

ttcacaagcc gcagaaaagc ctctcctacc ttaaaatgga catgtacgtg tcgcttttct 840

attgcttcct atctattcga caacattgaa gttttgttct attactttcg tattcaattt 900

ttaatttttg tttgagaata ccaattttgt tttggatcta aactcgttct aatgtaaaaa 960

ataataatca gccgcagtat atcaatatgt tacattgtat ctggacgtgg gatatctgac 1020

1080

1140

1200

1260

1320

ggtttaactt aattggttcg ttgattattc tttataaaag tgaaagtaga tttaaatttg 1380

ttttccaatc ttctatgttt ggccattgtt tgcaagagtt ttgggaatta aagtcttttt 1440

1500

tttattctca tttctaatta ttgttgacca aaaaaaaaaa aaactgtttg gttttgaggt 1560

ttaattactt attgtatatg tacactctca atcataa 1597

<210> 76

<211> 1552

<212> DNA

<213> Arabidopsis thaliana

<400> 76

tttactttca ttccccgct ctctttaagc acgtgtgata cagtcaccaa ataattggct 60

120

aattttttatt tcgtattact tactttagag ttttcattat agggttatta tttaatttga 180

acatctatgt gtttctcctc aggtagaatt catgtttgtg tatatatggg tgtctactat 240

ttatggaata tttatacaaa acatctaaac atatcattgc ttgaaaattc tatgtttata 300

tcataatagc aaattgttta tgtttagtta tatataacac aacaatagta ctctaagaaa 360

aacagcatca tgcacgtttg atggttgact agtaactaga cacacgaggc acaaaggtta 420

tcagttgatt aacatgtgta tgtatatacg gtctttcact tatcacatta caaacttgaa 480

agtaaaattt gtttagcgat attaagaaag tttatatata gagacctcgt actagctgga 540

aatgtatatc taaaatgaaa gtacactccc tcttacatac agtttaatat attttcaaca 600

taaaacatat gtacatagta ctagcttatt ttttaaccaa aacttttgggg tttgatagtt 660

taacaactcg gtcaagaatc tcatattttc ttacttcaaa taattgccaa tatattctag 720

ttctttttgt tttttaaatta tacctggtaa ttttctaacg agagtctcat aattgcaagt 780

tgtagtagtc ttgcagctgg atatgaagcg cgtgaattaa aactaaataa tacttacaat 840

aaatgaaagc aacgtatcat atcattttct agttgttttc agaagccgca gaaaagcatc 900

ttctacctta aaatgggttt ccatgtgtcg atgccttatt ggatcatact ttcttacttt 960

ttaattcagt ttaaaataaa ttgaaaaatg agataaacat ttaagtggtt caattatcat 1020

1080

ataatatttg tatgcctaaa tcaagttatc catgtttgaa ggtgtttgca gtgaggtata 1140

tatgtttttg tctaataaag attacagtta tgattatgta acataaatta gataatattt 1200

acagctcgcg atacactaca attgaaacgt ggttaattag tgaaactgaa atttgaagaa 1260

gaggatgaaa tatgaatgat attcgttaaa atgatctctt agattgtata gtcattgtcc 1320

cccattgcca aaagatcaac cacttacata aagtaaaacg catctttagt taaaagctaa 1380

ccaaaagagt ggaacatatt tatttttgga ttgcatttgc gaatttcatc aatatatggt 1440

tttgttcaat tgggaaattt aaataactcc actaaaatgt acaaagtaat tttttttcga 1500

caaagaatct atgaagctaa aaaatctacc cataagtcac ttttagcaga aa 1552

<210> 77

<211> 2001

<212> DNA

<213> Arabidopsis thaliana

<400> 77

catcaatcta tatgttgcat gttgtaaggt cacaacttgg aaccataaaa gaatggtgga 60

ggatagcaag tgcgtagaag ataaggatca ttttacatga taatctggat aagacatgcg 120

catatacatc aagtttccat caacaagatt tatagtaccc aaaagaaaga aagaaaaaaa 180

ctaatattcc atcgatctat agaagttgaa caaaataact aaggtaccaa gtgaaatatg 240

agtcagcaag caaagttaaa aaaaaagttg tgtgagacat atcacatgag taaattaaca 300

360

caaattaacc tgacaagaag catcctaaaa aattaacctg tcaaacaatt accaaccttt 420

aaagtgaaaa aaaaagttga aaacactaag gctttgagag cttaaatgtt cataaacaat 480

ttttccttttt cttgccaaga tcaatctgca atttttgtgt caagcatgta cgagttattg 540

tttaaataa atctagagtc gttatgtgag ttctactgta ttttatgaaa atcgattaac 600

tagtaaaata catcagtctt cttaagaaga gaacctaaac acgaaaccct ctaataaatg 660

ctagattgca tattcttaca catctatatt agtgtcaaaa acatgattgg actgatttta 720

tagtattgaa caagaacgtg actaaatttt cagaaattgg aaagaaaaca aaaacatgat 780

atatttcaat atctaaattt caccatatat atataaaaag acatagtgca tgtgagacgt 840

aaaaaccttt gtaaataaat tttctcttta aatagtaaat agtaccatat cagattaacc 900

atttcagagt gacataattt gtttgtaacc gtatagatcc gttttatgca ttaaaacgat 960

cgtgaagtag ctgtcatacg aaagaacttg tcggcaagag aagcaaagca taagcagaaa 1020

1080

acattataag tgttatctac ataatctaaa cgtgtcaata cctgaaaaaa caacaaaact 1140

ttatttgcac ttctttcatt tcatgaacaa tacataacca gatattagat aggacttgga 1200

cttgtgaata tgctctattt aaggcatctt tataggttat atttagaatc tttttcttat 1260

ttcttttttg agcatattat taaactatgg actagatctg atgaaagaaa gcaatctatt 1320

1380

agttgaagca ttttgtttgg actatagaga gagagaaaaa aagatcttaa ctctccttag 1440

aaaagtacta ggaatcaatt tttattttct gcaaaaacaa aagtgtaaaa gaaaattaat 1500

1560

1620

aagtttggaa gcatttattt ggccaagaat ggcttagcat ctctgcaaaa ccaacacaaa 1680

gctatattct cttaaaaatc agttcaacaa aagaagaacc tgaacacagt tggcccacca 1740

ccaccactac cacaatctcc tcctccacca ccaccaccta gtcaccgggaa accaattcat 1800

gccctagatt aagaaccaga atccttttga gacaagatct atatatacac tctacacatt 1860

tacttataca ctttatctag atatagatgt gtgtgtgtat aaaggttctt tttcattact 1920

tgttggttta gtgataaaaa ggaggagagt tttcagttgg gttttggttt cttggtgaga 1980

gaagagagaa aaagagagga t 2001

<210> 78

<211> 1558

<212> DNA

<213> Glycine max

<400> 78

60

catcacatca ctacttatat ctattttttt tctttttatt ctaatctttc tcgtaattaa 120

180

atttttatca gcaaaataaa ttttattaaa cttgggtaaa ggggtgccac aacttatgcc 240

catagtatca tgtcattacc ttcagtccta gtaccagatt ttcatatatt ctctaaacat 300

360

ggagacattc ttcgtatcat aaaacaattt cctttattat cttcttaaac tctgttcttt 420

ttttttaaaa aaaaaaatct gttaaataac tatttttagg agatgaatag taattgaatt 480

acatataatt ataagaaaat gattttacaa ataattacct tgagaacaat gattaagaaa 540

tcgataaata aaaaattagg taagatgcag tagaaacatt aattaaatgt caatatatat 600

atatactaat ttcttaacaa ttgatcaatc tcaataatac tgtcaaatgt gcatatcaat 660

tggcattaga tggttttagt ttagatagtg atattgagtt ttttttttta tgcaagtgat 720

attgagtctg tacatatggc ctctaaaaat aacactcaat gattaacaaa attgtatttt 780

actggtgaaa tggaagtatt attattaaat ataatttcag ggtattaaat taagaaaatt 840

gttgaattaa gtgactccgt tttatgtcat aaagcactaa taatacatac attaattttt 900

aatgaataaa ttttacgtct ctagtttaat attaattatg tactaatagt gttaggattg 960

ctatcaagga gagtctaaat atatcattta aggaaataaa agtacaaagt ttttctggta 1020

1080

taagtgattt aaaatcttta atatggtgta tttaatattt attaactact atccttaatt 1140

agcaggacac tacttaacaa gaccgtaata gttcaaaatt acgtcataga aagtttaaag 1200

aataatggga gaaatcataa attatgttag gattgatgaa aatatgattg attagaacga 1260

agcgaaataa aatttcaagt taatcatcat ttggattttt ttaaaatgca atagaatgat 1320

aaataatgaa atgaatttca tctcatttca tccaacccta ctcataaaat tatagtatat 1380

attacttttt acttgtattc aatcgcaatt ctattcttttt taaattaaaa actcagacat 1440

aattatcatt tctacgttgc attttttaca aacacgtcta cctacttaat tactcttgca 1500

gctagcttgt tacttttaac ttataagagt atcaacggaa catgattgct taaatgga 1558

<210> 79

<211> 1545

<212> DNA

<213> Glycine max

<400> 79

60

taatttatat ttgttttcta tcacatcact ggctttatct atttattttt cttttttatt 120

ctcatcttta tctaaattaa aatgtaaaat tttatgtatg agtaccatta tcaaattttg 180

aaagcaaact aaaattattttttttatcggc aaaataaaat tattttttta tcggcaaaat 240

aaaattttatt aaacttgggt ataggggtat cgcaacctat acacatagta taatgtcagt 300

gccttcagtc ctactaccaa attttattct attctataaa catattttca ttgtcgattc 360

aatctcttta attttatcaa attttcaatt gagttttatc ttcctctcat ccacattttt 420

ttgctggggt taaagagact aaattaatgt tattaatttg aagtataaaa attaactaat 480

tcgttaaatt aattcataag aggaaagtgg gattgcattg gagggtagca agaaagccat 540

tgaaagaaaa cgcagcagcg atcatttgta taaagtataa actacttcct acgtactata 600

tgtatgaagt gccattctaa catttttttt tcttctaaat tttatattgt gttatagtat 660

720

atgaaggttt aaactttgtt gttgacatta tacatagaaa aatacaagaa aaaaattatt 780

atcatcttaa actctgttct ttaaaaaaaa aacagtagtg ttaaataact gtttttaaga 840

gatgaatagt aattacaaat aattataaga aaattatttt actaatcatt actttgagaa 900

caatgattaa gaaatcaata aataaaaaat ataaagaaat aactatttaa cttgcgtttc 960

aatttttttt aagattacaa tattatagtc cgtgaataca cattttcctt ttgtgaataa 1020

aggaagtaaa cattggtagg gaaccaagaa gtctaaagca gtttgcttgt ttgccttaaa 1080

tttttcttta gctcgaaggc atgtgaaatg aatatttcat tgtgagtaat tatgagattt 1140

tcttccaatt aaggcaccaa agatgtaaaa aaatgaaata gtttattttt gtttaatcag 1200

1260

tagttttcat atgaattggc gttgaaagtt aaatcttcag atttaattca cgcattatta 1320

ttattattat tataagaatt ggattagata aaagaatggt taaattttatt attattttgt 1380

taggcttata cctttacata taatattatt attaatttgc agtaggaaac aatattctat 1440

aataagaatg attattaaaa tatgaaataa gaacttttca aaatctgaaa ttatgcagcc 1500

acgctgggcg agtagtatct cgctgagcaa gcagtgcgca ttaca 1545

<210> 80

<211> 2001

<212> DNA

<213> Glycine max

<400> 80

ttaaatttaa attgacgtgc tgaactgaca tttcgatttc aaattgacca caagaaaaag 60

agaacgcgta aacattattc atttatttta tttgttcttt atttatttct tctaaagtac 120

180

atggttgaag tgatgttaag attttagttg tgggatgatt tgttcttgaa taaaaaatac 240

ttaatgttca ttaagtatct tccataaaaa tatctttttc tcctcttgat ttagttattt 300

attaatatta ttgtgtaagt gtttataagt ttgtgattta tcgtaacttt agaatgaatc 360

aaagttctat gatcctaaaa atgtttacac ctagacctag ttgttaaaaa ataaactttc 420

attaagattc cttgtttact tttaaaaatc gacttttaaa ttttttacac atatttttat 480

gtcatttatt ttaatatcat gtaatacaca ttattaatcc caaattatac tctgtaatta 540

attgtaaaaa atatacaata ttatacatct acaattttgt tcgtagggta ttatatgttt 600

660

cccagcttat ttccattttt tctaattttt gtctctcggt aatttaattc agtcctctta 720

ttttataaaa tcctataatg ttagttccca agccacaata cgttgatcgt tcatgtgtca 780

tatctttatt ggatgatgac cgacacatat tatgttttta ttggtcaata aaaataacaa 840

tgtatttcat cttttatgca gttgacattc aatcacaaca taacatatga cagaatcatc 900

atctaataag aatgtgacat ctaacagtca acatcatttg ttaacggtca atatctaaat 960

gtgacattag aagactaata ttgcacgatt ttacaagata ggaggattaa attcgtgaat 1020

taaattaatg ggggaccaaa tttataattt tccctataaa aatattatata atatcaatca 1080

atttttaaaa aatgtttcaa atatatctcc taaattactt attattataa aaattaaaat 1140

tctcaccata taataatcta taactacatg agaatataaa attttatatt aaatccaaaa 1200

atatcggaaa gaataggggt agttgggtaa atgcatcatg taacatacga gctacctacg 1260

actatgaggg ttcacctcgt ttaaggttag ttcctgaatc cgaaacagtg agctgaaaat 1320

1380

tcatcgttag tcatcaggga ccctatttag ggcttctccc atttttcttg gaaatttcca 1440

ttaccaaccc ctccctcctc aatctcctta tgttccttaa tttggtcctt ctttctccac 1500

cgtctgattc ttcctacttt tcaatcaacc gcgttcccac cacagtagta tccacattac 1560

cctgaatttt aatttgagca gtgtttactt ggcccggtaa tcaaagcaca ggacacaggt 1620

gatttatgaa gcaaattaag ctaagagaaa aagacagcta gagagagaga aagagatatt 1680

tgattttgga gttagcatcc attaccattc ccatttgacc tgataaggat gcttttaatt 1740

tgctgggatt cttaataaca aggtagaata caattaataa tactcgtaca tacctcttag 1800

tatcacactt tagactttac accatataaa atacatacat gttacagtat atgttatata 1860

1920

1980 aattttgtga gtggatatta

aggagatgg gaaggggaag a 2001

<210> 81

<211> 2001

<212> DNA

<213> Glycine max

<400> 81

gagataaatg tggttttgta cctagtcaac ttccgcgtat gatatgagac acgattacat 60

taagcgtgac atgtacgtat tggaaatgta tgtgtccaag aaaataagga aatataacct 120

caaaaatttg taagagaatg gatttcccat atttgttcaa aagctaagct tacagctttg 180

ccgtgctatt ctgctattgc acagctatgt atatctatat ccaaatgcga tatatagcaa 240

aatgaaataa aatttagcaa gattaactta agtataataa catgtttttt tacttttata 300

360

420

agaagatcca taattacatg agaagagtat ttttcactct caagaaaatg aagagacgga 480

cttattaaat agataaataa tttttaatat atctatgttg aatgaacgaa tttagaaaca 540

600

agaagctatt ttctaaacaa tcttttttac aaacaatttt ttctaataat ctcttgtagt 660

taagaaaata gttccacatc ctagcgtagg gaaaaggtag ctaagtaggt aggtgctaga 720

aattgtaacc ttcaagatca catcttattt ttcattgtac tgatgagtga tgacaacgtg 780

agtttcaatt ccaaagataa caactaatat tagggatgtt gacaattata tataaatata 840

tatagattca attcatatct acgaaaatta cttatgatat atagcaaggt aattattatt 900

attaataata tcaagagtat gaagccggat aaatatgctt tttttaaaac cggaggatac 960

tatagtaacc ttcatatata gatctacccc attgccaccc taattaataa tgtgctggaa 1020

aatatcttac tagtcacttt caaatttcaa ccaaaaatgt aaatatacat tatttctcct 1080

attgattgtt ttcacatcaa gtactaccag ctttccattt aattaccact tactggcaat 1140

gaaaaaaatc tcatctgtta cttttccaat aattctgcta ttttaacaag tgaagaacaa 1200

1260

ataaagtgac acgtgtggaa tccattttaa ggtatgtgac gacgttatga tactcttgct 1320

gtgcctctct gtagacagta gaaaatgaca cagattttca ctgcaaacag aaaattatag 1380

1440

aaataatatc atttttgtaa gtaatagtat ctatctatat gacagctgtc actacgcgcc 1500

agttttctcc cactggagca acgagtgttg agtgtctcac acgcgcctat gttgcgtgtg 1560

ggggaggtga ttgtcttcca aaaatacgtt aacatggaaa atcatgggaa agagaaagtt 1620

acatgaaact agaaagaact ccaaccctaa gctgcactcc atttttttct ggtggagatg 1680

gaaaatcctg aggataaaaa ggaaacttag aaaagagagt acatatacaa tacaagacaa 1740

cttcacacac acaacacttt cctctctctc tctctctctc tctagggttc cgagtttgaa 1800

gttgaaagtg ttgaactatc tctagctagc ttttcacaaa tttggttgca cttgcaactg 1860

aggcaggaaa gaggggcacg agaggaggaa gaggaaagag aagaaaaacc aagaaaagtg 1920

gagaaaggga taggtgttgt acagaaatag aaattaaaat tgagtgaaaa ttaaagagaa 1980

gaaggagtga tagggagata t 2001

<210> 82

<211> 1643

<212> DNA

<213> Glycine max

<400> 82

taaaaatata tataacatgg ttttaaataa taaaacatta aaataaaata ataacgtatc 60

aaataaaatc ataaaaatat actaataaat aagtcaactt aatattattt attatatatt 120

180

240

300

ttttaatgaa atcgtaaata attttataac tttgatttaa agtaaaaaaa aattacgact 360

ttaaaatctt aaaaaaaata aaatttaaac tgaagttgta aataatttta cgacttcaat 420

taaaaaaaaa taaaaagtca tatcagatgt tacgactttt gagttagaag tcataacatc 480

tgttataact tatcccattt tggataattt ttcaaaatat acaccttatt ggaaaattgg 540

gtaaaatttt acccctaatt gataaaaaag tcaacgatat aatttcccat ttagattcca 600

accattaaca gatttttaat tgatgaatag tataaattttt tatagttgct ccttataaat 660

taataacttg aaaaaataat gatatttaat ggaatctgat taaaattttg aaaaacaaaa 720

tataagaact ctgtcaacag cttttaaatt agagatttat cttaaaactc aataaaatta 780

taatatatgg atcgatctaa attagtaaat tctgaatagt actaatgtaa ccttaatact 840

attattatcc atgcctatct gaaagactaa attcccgttt tgcatgctag aaaatgaaag 900

ccagtgataa agtgacacgt gggggtccag tttaaggtat ctgacgacgt tcttgcagtg 960

ctctactaac gtgaacacag tggaaaaaaa tgacagaatt ttcttaatat catccacagt 1020

aataggttat 1080

aatgagcgtg tttcatacgc gctataagtg agaacgggaa aatatttttt gccaaaaata 1140

ccttaaccca gtaaagcatg ataaaaagaa acttgtatga aacaggaaac tcggaccaac 1200

cctagcaaga tgcactccat ttaatggtgg agattggaac aaagagaaaa atagaaaaag 1260

1320

ttgttgttga tgatgtttgc tttgttctct ttctctctcc tcaaattttt tcctctctct 1380

ctagggttcc aagagtgtct tgaactactc cagaaccctc tcttgttttt cacaaatttg 1440

ggtttagtag agtactgacc cagaaaagaa ggttcaacag gtacctaaga gaggggatag 1500

1560

gggaaaatat tttgaataag gaagatataa ggcaatatat aaaaatatta gtatttgata 1620

ataattaggt gtagaaatat tat 1643

<210> 83

<211> 2001

<212> DNA

<213> Glycine max

<400> 83

agctgtttca cacaaactat taattactgg ccaggttaat tactcaagaa gtagtaatac 60

tatcagtatt gatattaggt tatattttcc tattacaagt ttatattgtc ctgtacaact 120

ttgtagtttt actaagtttc taaactaagt aaataattac aatataaaaa aatctttaca 180

ttactccgtc tatcctataa caattattgt cttagaccat tttttataca aataaaaaaa 240

ataaaaaagt ttaattactt atttacttct tatacttttt aaatttatct tttttagttt 300

360

cctgcagtta actttttttt aactaacaaa gttaaaaaaa ttaacgagat aaataccttt 420

taggaattaa aatataaatt tgaaggatta aaaaatttat ttattaaatt ttagagacta 480

aaaaattcat ttattaatta taagaactaa aaggaataag tttaaaaatt ataagaatta 540

600

taatattata taattattaa tttattttta gattttgtag ttgatcgtta atatagccaa 660

cgttcctgct gcaagaagct accccggata ttccgtcccc atctaccaaa acaatcggaa 720

atgtttcaga ttttatagtt gatctcttaa tatataagag atataggtgg aaaattcatt 780

aatgttatat taaaaactaa aattacaatt atattaggat aaaaatattt tcttataacg 840

900

aattatttta aaatcattta taacagattt ttaattaatc cgtagtataa attttttatg 960

ctatttgtgt atcgaaatta aactatgtta aattactcct tataatttaa taacttgata 1020

1080

actctgtcaa ctgcttttaa attagatatt tatcttaaaa ctcaataaaa ttatatgtaa 1140

tggatagatc taaattctaa atattaatgt aaccttaata ctattattat ccatgcctat 1200

ctgaaagact aaatttccgt tttgcatgct agaaaatgga agccagtgat aatctgacac 1260

gtggggtcca gtttaaggta tctgacgacg ttcttgcagt gctctactta cgtgaacaca 1320

1380

1440

acgctctata agtgcgattg ggtaaatatt tgtttgccaa aaatacctta accgagtaaa 1500

gcatgataaa aaaaaacttg tatgaaacag gaaactcaga ccaaccctag taagatgcac 1560

1620

ctgaggataa aaaagccaag ttagataaca ggagagatga acacaactgt gttgtagttg 1680

ttgttgttga tgtttgcttt gttctctttc tctctctctc tagggtttca agagtgtttt 1740

gaactactcc agaaccctct cttgtttttc acaaatttgg gttcagtaga gtactgaccc 1800

agaaaagaag attcaacagg tacctaagag aaggaaagag ggggatagaa agaaagaaaa 1860

agaaatagaa actgcacgta taaaagaaga aagaaagaat caaagaaaat aggaaaaaag 1920

ggcaaatatt ttgtttgaat aagatataag gcaatagtaa tattagtatt gttgataata 1980

attaggtaga aaaaatatta t 2001

<210> 84

<211> 2001

<212> DNA

<213> Solanum lycopersicum

<400> 84

60 aataagatga aagttatttt tacgctaaat aagatgaaag

taagatgaat attttatttt aaagctaaat aagatgaaag tttaagttaa atgagatgaa 120

catttaaatt ttaatatata cgacataatc atggcaatac acacaaaaaa aatatttaag 180

ctaaataaga ggtaattttt atttgtaaga atgaataact aaatcttgta caaaaaaaaa 240

aaactaaata aggtgagggt atttttgtaa acaaccaaca acttattctc aaaaaaaata 300

aataataaat attaatttaa atatgacaaa ccaaacaaac aactaatccc tatacaacat 360

attccagcat aactagtatt caaatcaaac aacccctcta tgaaatccat aagacaagaa 420

ataaaacaaa attgcaaaac aatcaataga ttaaggtcta ataagacata agtagataag 480

tgaatcgttg aagaagacat tgaattttga tatagtacaa ctccatacct ttttatggtt 540

attcatgtta atcttagggt attcgatata actttacaag taaaatctgt aaaagatata 600

cattattctt atctttattt agaaagatta tttttgattg actctcaaat caaaaatagt 660

gtaatcgagt atgatttttc aaaaaaacaa aaaaacatca atataacaat atgagcgaaa 720

taaactaagt aataggcatt gtaaaaaagg aagaaaaaga tgcttatgac ttatctccgt 780

tactgaattg gttttcctct tctccctaat aagcaaagag ccaaagacta atattgaaat 840

gagttatttc ctcatatgat attcgacatt aatcctttca tcatcaatta gttttttcga 900

atgaagttag actcaaaatc ccttgtgaaa ctttcaaaaa attaggttta tgagttgagt 960

tggtttcaga atgctaaaaa taaaatgcat aatacatata ttgtatccta aatttggctt 1020

caaattttaa ctttgacctc taactttcat aatgcacaaa caaacacttt gactatctaa 1080

ctttcaaata aataaacaca tgagtcttac atgacaaaaa tatacatacg acaccacgta 1140

ggacgaaaaa tgacatgtaa gacatgtgtg tctatttgtt caattttata caagtttaac 1200

tgtttaagtgtctacttgtg cacacccaaa gtggaagggc

1320

agctaaataa atttttgtta gtaagttttc catttctata aatttacagt gaagaagtga 1380

gttatttgct tacgcatgat atgtacgtaa tatccactaa tacaccgacg cgtgtccagc 1440

1500

ccaacaaact aaccaagcgc gtgtatccca gacaacgaat agttgttgtg cgtgtactcc 1560

acgcgcatca acagaagcgt aagactagtc cctataatgc attggctaaa tacatgaaac 1620

1680

acctaagcaa atcctaaagt taaaacaatg aatccctaaa gaagatagga agaaatgcca 1740

tagataaaaa caacccatgt tcactttttc tctctctaaa cattgaaatt caaccaaaac 1800

1860

agggtttgca tttctttcac aattttggtt gtttcagtag gagagaaaag aggatctaag 1920

agttagccaa gagaagaaat tagtgagaaa ataaagtaga aaaagatcat cagaggaagg 1980

agggatgggt aggaagag t 2001

<210> 85

<211> 1817

<212> DNA

<213> Solanum lycopersicum

<400> 85

ttacaccccc actaaaaaac ccaaaagtta aaacaaagag catctaacta ttaatatcac 60

gttagaaacg tgcgatcatg tcatttaaaa gtttatatcg atagagatag tacatttttg 120

ttatttaatt atagggggaa aaaaacaaat atatctttga attatcatta ataatatgca 180

gatatcttcc gtcatacttt tgggacgata ttggtgtccc caccgtccaa aaactagagc 240

atatatatcc tttactctaa taagactgaa cagagacacg tgacacaatc atattcattg 300

atcatatatt caatatgaaa atgacaagtc ggtggataag

gttagtttaa agggtatata tgttctattt ctgggtggca gggcaccaat atgtcaaaag 420

tacgacaaaa acgatacttg ggggttatat ttgtcttttt 480

tcccttaatg gtatcctcaa caattgtcaa gctaataaag cgctacttct aattattcaa 540

atgatcgagt tattttatgt atgttaatat tgcaagcgac ttaatatctc caaacattgt 600

tcgaactcag cacgtacaat tatcaaacca aagagcaaag taggtctcga caaaacgaag 660

agtatcataa tacctcgttc gttattcttt cctaaaatat aatttttatg ttgaaagtaa 720

aaaaaattag cataaaaatt aatctattac gactgacgcg ttgttcaaag tcgagagaat 780

aatttaaagt ggtaggaaat caaaattgat ttgggtattt

ggcttttttt gttttaataa ttcactagga atatattctc ttttttcaaga taaattaggt 900

tttgcaatgc tattatatat aaatagaaac attactagca aattttttga gtgttagaag 960

1020

aagattatta aactgtgaaa taaaaggatt gtgttggctt attggacggt ttttaattaa 1080

tgaaggaata ttggacggtt gctgattgat gaagacagaa aataatttga acgaaaatga 1140

1200

1260

tagcatccta tggatcacac ctaacaaatg tacataactt 1320

cttctattct gatttttatg tctcattttt ctttttagtt attaaaaaaa atatattcag 1380

ttttatttta tttttaaaat tctcatttca cactttgtaa ttattaatta tttttttaa 1440

ttcattatca aattatatcc tataaagtag aactagtgta aaaacaaatt atctttattt 1500

ttaatcgagt caactcgtcc ttcataccca aaatgtatcc cacactcact ctattaacat 1560

acagtgagta gtacatgata agtttgttaa ataaatttta taccatcagt acatgcaatt 1620

ttatgtcaat aaattacaaa tatagatatt tgtgtagtgc acccttcttt tatagtaata 1680

gatttttaga gttagctagt aagtgtataa tatcccttga tttaataaca attattcact 1740

atattatatt gagatgttta tcaatatttt tttactttat aaaattaata aataatttta 1800

tattaagttt ttaattt 1817

<210> 86

<211> 2001

<212> DNA

<213> Solanum lycopersicum

<400> 86

ttcaagaatt tatgttctta catatttatc tatctatcta tctatctatc tatctatcta 60

tctatctatc tatatatata tatatatata tatatgaagt ctcccactta tcggtaagtg 120

taatttcttt tatttttttt aatctattaa caaatttttta aatttttccc tgagccataa 180

caattgtata atttaaatat gttctttttc ttacgaaatg tcataacaat tattctcttc 240

tttattttta ttttaatatt gattaaagaa ataataattt attattttct caataattca 300

tttattagaa attcgtgagt tatgtgataa tgatgaataa tgaaatttat gtcaaaatat 360

420

aataaaggat tatttgcaag ttaataagaa aaagacagct cgtaagggaa gcatcgacac 480

gttggtaatt aaacactggc gattgtacta ttacgttgat gttcgtgtcc aaaaggtaat 540

acgatgtatc aatgtgaaac attaatttac ccaaaaccac aaaaaggacc tacacttatt 600

acatataggg taactccatg tacacctata tatatacata taataataaa ataaataata 660

tacattcata aataattgca tatatatgta gagacacact ttttaaggtg tataaaaata 720

taacttttta gagttcagtt ctcaggtgaa atttattagt atctgctcta tcattattaa 780

taggtctatg tttgagaact atcaccaagt tgattgggct attttagaag ggtagaggtt 840

tgacacaaaa gttaatttag ataatatttc taattgatga atataagaaa tatgttttta 900

ttaatcgtac agatacttat agttttactt aattttgcta ttagattgat ttgtacaatt 960

cgtcttataa gtataaaatt taaaatttgt ataattttat ttttgattat ataaatcaag 1020

1080

ttgtcttgtt tgtatactga gaaacgaaat atacaaacaa atttctgaaa aaattctgac 1140

1200

aagcagacat aaatatgcat accaacataa atatacaaac ctcaatctta atagcaaata 1260

1320

tatttgctat tagtaagatc atgaggggga ggcagatgtg agttatgagg attcactagc 1380

tttgactaaa ttgttaaata gttaataagt atacgtaaca aatttgtgta cttagtaggc 1440

1500

aaaaaatcat ctcttaaaat ttgtaaaaga attcatattt gacgtgaaaa gattattacg 1560

tcatatcagg gaattaaaga ataaccagtt taaattatat tattgtttat ttattgacta 1620

gcggatattt atttaattat attctcagta ttattttata tgacatcatt tttttagttg 1680

atctcaaaaa aatgttacct tacttcacac aaatattcaa gatttatatt aaactacaag 1740

tttccaaaaa ataaaaataa ttcttaaaaa taaaaaatgt gtcacataaa atgaaacata 1800

tatatttaaa catttatg tgacttatca atttgagttg ggttatatga aagtgacaaa 1860

cgattagata gggcataaca gtgcttctct ctctttccat taaatttggt agtccacata 1920

cagagagagt agaaaaagtg tgttaaccta gagtggaata atagtgtttt tcttgttaga 1980

gacaagaaaa gagagaaaaa t 2001

<210> 87

<211> 1882

<212> DNA

<213> Solanum lycopersicum

<400> 87

gttatggttt tggttcttttt ttctgtcctt ttgttcttag ggtaataaat aaatagcttt 60

gttgggccat ggggtttcct aatattacaa cattgtatct atttttctta tatctgacgt 120

ataaggtata ataatttcag gataaatttt atcgtgtgtt tagttgaaag cttaggtagt

tattcttaga ttatttctcc taccccattt atattttaat aacggaatat gttatctcat 240

atgtatagca aataaaataa taacttattt tgaagtaatt aattaattct gaaataattt 300

360

ggtaattctg caaactgctt ttgttatttc ttctgcctgt gtcccttgtc tctctataat 420

taatatataa gtaaaatgtg ttttgatgtt tagggccaaa aaaagaagac aagtcaatac 480

tattaaccat tattattttt ggtttctcac caactaacct tttaatatca agtttttgcc 540

cctattttgg ggtggtattt aatttgtatc aaattacaaa ataaataaaa ggagacataa 600

660

agaattttga agttagattg atttggaatc aaaattaaaa ttttttttaa ataggtaatt 720

agattataaa aaataatatt tttattggaa aacaacataa tttgttgaaa actattaaat 780

attatgaaaa ttttaattat atatttttat acaatttttt aaatgagtaa atcacgattc 840

gtaaataaag tatcagaaac gtttgtaatt acattttatt aaaaagtttc caattctcat 900

tattttataa actactaaaa ataaaagttt ttcccacatt tccattttaa tggaaattgt 960

aagacataat attaaaggtt gtaggtattt gtatccctta ctgggattgg ggagtgtttg 1020

aaaagacctt tatatggaga tacattatta gccccttata ttggaaatat catatctata 1080

aaattcaaaa gttaattcat gacgaaaaaa aatatctaaa ttatattaaa gagatcacat 1140

attttttcta gctatgactg gacatatagt gcgtggataa ttttagtatg aagatctaac 1200

atcaaaaagc aagaatggga tgaatcgtgt attaatatca tgtaaggagc gaactcataa 1260

gaaaaaaatt gttcagatca tattaaaata tataccaatt cttaaaccat cgagacggag 1320

acttttcaca aattacatac atactagaca ttataaatat ctttttacat catcaatata 1380

tagtataggt aagtatatct tactacacta tcaatttaaa ttcatcaata attactgaac 1440

1500

1560

1620

gggacatacc acatgtttct gttacataag ttggatattt tgatattaga tacctaagaa 1680

tgtgtcatca tgttactcca tacatatcaa acaatatttc acttcaattt gaaatattta 1740

aaatcaagat ttgtaatcac ataaactaaa atacgttaac gtaattaatc aatcttgatt 1800

1860

gaattgatat tcaaatacct aa 1882

<210> 88

<211> 1574

<212> DNA

<213> Solanum lycopersicum

<400> 88

aatgaaaaag aaaaaataaa gcaatagatc ccatcttatt taaaaaggga aatagtacaa 60

120

aatttatgtg gggttagtgc gatgtattta tttcaaaaat aataatgtgg gtgaaaatagt 180

gatctttcta ttaaattaga tattttatgt atacattttt tgtcgaatta atataatatt 240

ttctcattaa acttacatgt tccaaaagga tcgaatgatg tacttggttg aattgcgaag 300

taattttttt tagagaaaca acgatcaatt ctcacttaat ttttttttta tattcacgtt 360

ctaaaaatcc taaatctaag aaaaaaatgg gagtaatttg gaacttaaat ctaagacaag 420

tcttaattat gtcttgttta gctaattaaa ctatcaaaat aaaaatattt tttatttaaa 480

atagtatcca aaaaagaatt acttttggag agtcaatttt tgaatacctt gttaatttga 540

taaatatttg tatttatatt ataacaataa tttatacttg aacaaagttt tgaaaatact 600

aaaatattaa aacccatttt ttttcttaaaa 660

gtttggccaa aacacttcaa ttttcctttaa aagttggcca tactaactac atttaatttg 720

tacaaatcaa aattatttta taactcatat cattggcgga acttgacgtg agttgtctaa 780

aataaacaaa gtttaataaa agtgtataaa ttaaaattac tgttaacttt agaaaaatac 840

caatgagttt gacctttttt acaagttgaa caatacaaac tttaatcaat attattaaaa 900

tatattttca tttttattgat ataagaaatt actacaattt ataatacttt tcctataatt 960

tcaaattttt aaattttctg aaatattaaa ccaatctaat ctaattcaat ttaatcaaat 1020

taattcatat aaagcaaaat taaataggaa aatattccat atcaaagtga gtagtttctc 1080

1140

1200

tattatttta caccataaat ttcaaatctt ttcatttttt cttcttaaat attatactaa 1260

atcaaataat atcatataaa ataaaacata aaaaaatata tattttgaga aattaaaaag 1320

1380

agaataggtg tggaaaaagg tgtaaataat aaataaacaa tttaacaagt actatgaaaa 1440

1500

ttgattggtt gaaaggacaa catagtatgg tactcatttt tgtatggtgg atatataatg 1560

tctatcacag attt 1574

<210> 89

<211> 2001

<212> DNA

<213> Zea mays

<400> 89

agtctcttgt tgggctggac gcctccatca aggcgcgagg tcaagggcac agttaaccgt 60

tgtgctaact ggtggagatc aattccaaca cactggacta gtgctgggac taacttaaaa 120

ataggagcct aaagcacggt ccaacaagaa ataaaatagg tcgggctacc acgactcgaa 180

ggtgggtcta gacctgacct caagttacgg cctgttgggc ccagcacggc ccacacatat 240

gggtcggttt gggcctacac ggccccaatg aagcctatat tatttaattt ctttagtttc 300

gtaaatttat aaactttata ttgttgtgat atttggactt tatgcggtca aatgatgcta 360

gcattgttta atattgtggt tgcaatattt ggattttacg aggtttgaat atataggacg 420

agcttggacc ggcacgattc aacaaaagca cggcttgctt tagagtagaa ctgaccattg 480

tttctacttt tcaggccta aaagtttttt tttatcttct tagcccgatc ccagcactag 540

actggactgg actgagacct tgtactttga agtaagatgc atgagttcgt tatgcggatt 600

cgcaaggcgc gcgtacagta cagctcggca cccaacagct agtagtacgc acgttccgtt 660

aatccgctgg atggatggat cgatcggaga cggacagggg cggacgcgcg gcaaacgtac 720

ggtgcagtta ttgtcgtccg ccggatcgat caatcgaccg gcgcggcgga cggatcgaac 780

agtgcccgaa catgcacgtc cgtcccctac gcctgcggcg tgcagcatgc gcgcggatcg 840

tagtccccgg tcgatcggat gcgcgggccg gaccccggcg ctgccgaccg ggaggcggga 900

gacttggttc gtttcgcacc tgctgccttg tctcgcgctc gcgcggcgcg tcaggggtgg 960

ttggtcgcgt acttgcgttg gctgctgcct gggtggctct ccgcctctcc tggccacggc 1020

gagactgatg cgcgcgctgg cccagctttg gtcgctgttg cgagctggtc tggacagcga 1080

cccggcccgg ccggccggcc ggccgccgag accgaaagga agcaacgtac aaccagcagg 1140

1200

acggaatgga ggacacgccg ggcaggtcgc tgtgcgcctg tgcctgcgtg cgcgatcgcg 1260

agtggccagt caccagcagg ccggccatta aaggagagca cgtgacggcg cgccagtcgc 1320

ttccttcgct tcgcttgctc gggcgccggc ggggaccacc agggtaaaag ccgagcgcgc 1380

aggacgcgac ggcgacggcg acggcgacgg acgggacggg tcccatgagc ccatcaccac 1440

gagcggcgtg gacgtggagg tggatggaat gaccgatcga ccgatcgatc gcgagtgatg 1500

actgatgagt gtggcgtgac tccgatccct gatccctccc catccctagc tttccggcaa 1560

cgcgctaccg ggccgggggc ctagggtttc cccccctacg gatgctttgc cggaaacggc 1620

aacctgacgc cgaggcgcgc gcaccacccc tgcgcccacc ggctccttcc ctgcgccgcg 1680

ctgatgataa ctcagtccct gcacaggccc cggccccggc cccagcccca ccaccgctac 1740

tccactaggc cctggttgct agccagctcg cttgcttgct tcgattccta tcctagcccc 1800

cgtgccatcg ctttcctctc gttatttagc cctccgttcc cgaccctcat cctccgctcc 1860

agacttccag catctccgct ccggctgcgc tctgccttgc tttcctgcta cctgctctag 1920

cgcgagcgag agaggtacgg cggccgatct ggcggcgcag gcggagggct cggccggggc 1980

cggcaagtcg gcgccgaaca t 2001

<210> 90

<211> 1032

<212> DNA

<213> Zea mays

<400> 90

acatgaggag tgggttgatt ccactaaaat tgatggaatg aacttattat gcatcaactc 60

ataaagcata gagtgattcc acaaaccaaa cacaccatta gtttattgac caacctcagt 120

tctttgatat ataaaagttg tttgatccgt gtgcaacaaa tagtttttca ggaatttgggg 180

acgtacccca tctcttaaat gtctagactg attggacata ttaatctaat tttagtagct 240

aagtattagc tctcaaacac ctcctgggaa tgctgcccta aaaatggaat atcggtacgt 300

cggatcggag aaaggttggg gtatcgtggt ccaaagcatg cgaaagcaac ggcgcaggggc 360

tggaaacctg agaccaggtg aggtgcacga cgacatgcat atggtttgggg ttaggcctag 420

gagctggttc tctctccatg catggtcagc tcgccgcttt tgccgccttc gtgtatggct 480

tgccccatgc catgcatcgc gccgtgtaca cttgtggcgt aggggcgggg ccgccgcatc 540

ggagcgcccc cgtttcggca cggtcctccc agttttaggg taaacccagc tagggtagtg 600

ggggtaactg gccagcgcca ctccaaatct accctccttc aatttaaagc tgagaaatac 660

tgtagtatat actagtag agcgagagaa gggagatgtg gatagatggg tggatgggga 720

cgcgtgaaaa agatgcgaga gagaagagac gaccggacag gcagccacac acagtaacag 780

tagtgaaccc tgcccctttt cccggtctct ccactgatat tccgctcctg tccctgtcct 840

ccccggacgg agctaataga gccggctctg ctcatcatta tatcgtcgcg cacaaatgcg 900

aagcctagca gcacttgtgt ccggcggcct tgtgttcgtg ggggatgtgt ggcatattag 960

ctagctgata agcggccggc cgaagagcaa gcgcagtgag aagaagaagc tcggatcgga 1020

ggaggtcggc at 1032

<210> 91

<211> 2000

<212> DNA

<213> Zea mays

<400> 91

tgtctcgatc tctcctctcc tctcctctcc tcccgttggt gtgactgtag tagatccttt 60

120

gcctaggatc cgtaacgggg aggaaagaga aaaaaaacta aggatgatta tggataccgt 180

gtaataactg ctaactacag ttagcccatc tcagcggact ctctgcccta tattgtatgt 240

cactttctat tataaactac actatacaac ctatgatgta aaataatgtt ttgcacgttc 300

atatataaat cagtcgaaga aagggtgcct cactacaggg aatggtttct attggacacc 360

ttagcattca atcagtcatg tcccccccccc ccccaaaaaa aaaatgcacc catccagtcg 420

atttttgtca tatttgaatt cggtggtgct ccatgcacgc gtacctgctt tgaccaattt 480

atacgatcaa tatataactt acgttcttac ggttcttaga ctttatgaga ctttgcaagt 540

atgtttggat acaaatcaca ctaatgtgca tctttgtaaa ctaaattctt ttgattaaat 600

ttgtaatttt aaggtttaac ctgtttttgt tgtgtagacg acgttaggca ccgatcgtcg 660

cttcgctata tatctttgtt gtagacgacg ttagactcct agattaaata agcgaaaacc 720

gatcgtcgct tcgctatctt tgtttatttg tttgtggctg ctctacgctg aagagcccac 780

aggccacagc cccacacgac acgttaggca cccccaccca ccatccgcgc ataatataag 840

ctactgcaaa atatatgccg gcggagcccg agcgagcttt gtacttgctc cgccgtggcc 900

tggctccagg atgctttgga tttcgtgcgg cgccgtacgt ccaggcaaac agacaagtgg 960

agctgcatgt cctaaaagcc cggcaatcaa acacgctcta gcagcagcat ggatcacaga 1020

tatcagtcat ggggtggcgc tggcgcgggt gggtggccag gtggaggtgg gtgcatgtcg 1080

tcgtcgtcgt cccatacaga aattggctca cgtatgtata cgctgcgtac aggcagtagt 1140

acacaattac tagcaccaat gcaatccaac ggatggatct tcgcacaccc gccacccggt 1200

taaattaagc tactcctacc tctcccagtc tcccttggcc tgcctctata tttttggggca 1260

gcctccacca gccgggcgga tggggttgga tcgtcgtatc tgaggcggcg tggtcgtcca 1320

aggcgaaagc aacggcgcag ggctgggacc ctagtaggtg catgaggtcg tgcatggcgc 1380

gcgagatgca tggtttgggt taggcctagg aggttctctc tccatggcat gggtagctcg 1440

cgccgcttgg ctgccgttct cgtgtatgcg catgcaccag gcatttgcac cgcgccgtgt 1500

atatttctgg cgtgggggcc ggcgccgcat tggagctgca gccccgtttc ggcacggaca 1560

cgggaccct cccgttaggg taagcccggg gcagtgggta actgcccagc gccactactc 1620

1680

atgtagacgc gtgaaaaaga tgcgcgagac cggcagcgtg tgctacaggg gcagccaggc 1740

acacacacac acagtgaccc tgcccctttt cccggccgtc tcgctctcca ccgatattcc 1800

gctcctcctg tccagtcctc ctcccccgag ccggctcatt atatcgtccg tcgcgcagca 1860

caacgcaagt ttgctagcgg ccggatcagc agccacaaaa cgaggagagc aaccacgctg 1920

cacacagaga cgcccgtgtg tgagatatag agcaagctcg atcgaaggaa ggagggaagc 1980

tagagatcgt acgtcgccat 2000

<210> 92

<211> 2001

<212> DNA

<213> Zea mays

<400> 92

gtcgaaacgc cgacaatttg ttacacggtt accactgcac agcgagtacg acaacaagcc 60

taagatagtc cacttcttcg cattcctata cttctcactg tgatctacgg cactacgaca 120

ctatttcgag gcaaaaacag agggaagatg cctaaccaaa attctaatga agaattacac 180

cgtgaatgat ggcggcggtg gacgacggtg gcaatggcaa aaccctaacc ctgcatcatt 240

ttcccttcac ctttctattt tttcccgtg catagcatgg cagtccctcg acaatagaaa 300

cacatgtaag gtttcagtgg atatatccca atcgttgtac ttaagcagca ttcataaggt 360

gatttagttt ttttccctaa atcttgcaac gtgcataact tcgcgattga aactttaatt 420

aatacatgta atcagtcgaa tggaatgcac tggtttttag gcatgtatat gacctcaaat 480

catccacgca aggaatggat aaaatgacaa atcttcattg tcttcttaaa ttcaggcgat 540

cttcgtaaaa acagttgatt catataaaaa tagaaaatat atttggagaa attttctgca 600

tgtccttgta attttgctta gtaccatatt tatcatcgta aatgttataa tcccttctat 660

ataccactct gatacaattt cgtacctttt taattatgcc aacgcaagtg ggcgagtgat 720

atagtaggga ctagaatatc tgtgatgata aataatgtac tagacaaaat tacagtagca 780

tacatgaaat tgtcccttat atttgccatg cattttttgc tattacatct tagcataata 840

atcttggaag cacatcgcat gagttatcga ttcaagttag atatatagat gcatagtaga 900

caacatttag ttacgcaacg gcatctggtt ccaagaggag ctttgcaaga gatcaattga 960

1020

gataaaaaaa tggagatgat catcgtcctt tcagaatcgc taacagatat ggaagtgatg 1080

1140

ccggatccct aagcagaaaa cggatcgtat agcggtctcc tttctggaaa aagcgacgca 1200

aaacggatcg atgacctctc tcaccatgtc aacccgtgct gaggagctcc gtcctgtctg 1260

cacgcactat ggcaatggcg accggcctct tccgagctgc gaatcataac ggcatgtctg 1320

caagctcgat cgctgcaagc atgccttccc gttggaagga tcgagctgct cgatggagga 1380

aagaggaaag cagcgcgccg cgcatggcta tagcagttgg caggtgaggg cctctagcgg 1440

caacggggggc ctttgcccaa aatcacggca agccgaggtt ccacctggaa aaatccactc 1500

cgttgctctc tcccttttct ctctctctag catcgacctg tcgaatcctc actggtctat 1560

tcagttcggc aacgaggaga gagatagaca gagagacgga cgggaggctg acacatggcc 1620

ggatattggg gtgcggaatt gaatttggtt aatgcaaaag gtggcgtgtg aggacggacc 1680

gacggaggca atcacgagac gggaggggtt ggccttttgc ctctagggtt cggggtcgcc 1740

caccacctta ccggaaatgg caatgcggga cgcgccccaa tcactcacca caccacccct 1800

ttcttctcgc cctttttaacc caatcgtctc tccaccaccc aactcctcct gcctgtgctc 1860

tcctcctttt tctccactcc gcttttgctt ggttccatcg ggccggagag gagaagctag 1920

ctaactagca gcagctctgt tggtgtgtgt gtgtgtggag ggtagctgca gctggggcta 1980

gctggaaagg tcgggagcca t 2001

<210> 93

<211> 668

<212> DNA

<213> Zea mays

<400> 93

atcgaagata gggttgctcg aaacaaaaaa aaaatctgca gccgcagact gtatgcccta 60

cgctattttg ccgtaggaga gttggaacct ttgatgtagc gtttgtacta ctcggggtac 120

gctatccact atttggttga ggtatggtgg gaaagaaatg ataaaatata tggttgttgg 180

tgtagagttg atatatgaaa taaatatgac tgcagatgat tattgtgata tatggtaaat 240

acttttgatg ataaaaataa aatatttttt tagagtagta cggatagcct tatcgaccta 300

tgtacctggc agcgctgtga tccatcagct agcgaccaat cactcgccca ccacggcatc 360

gccaggagct gttgcttttt gcgagctgca tggtggggggc ctccatcgaa ggcgagggga 420

cggggcagag cgccctgatc gacgcgtgat aaccgattga tgtgccccca gttcgcccgt 480

accagcgcgc gccccctcgg cctcgtcctg cgtccgcctg cgtgctggtg cggcgcgctc 540

gcgctggcgg acgtccagac cgcaataatc tcgcggcctc gcgcgaggag gaccgaggac 600

aggaggtgcg atgcgatgca ataaaggttg ccgtacgcga gtgagtcggga cagagagaga 660

gagagaga 668

<210> 94

<211> 718

<212> DNA

<213> Zea mays

<400> 94

ttatttggtg cttcaccgcc ggagccaggc tagctataca ctttgtagtt gtttgtaggg 60

gtatatagga gcagtatcta cagtagcagc actcttcacc ggtccagcta gtaaatgcgg 120

tatctgtcgt ctgtcgagct aacctatcat atcagcgcca tgcgccccgg ccggtcgtaa 180

cctagctagg agtagctagc taggactagc tagctaggag cctaagcgcc cagcccagtg 240

ggtggtagcc taccaagggt gaggaagagg agctgagcta ggaccgcgag gcgagcgaga 300

tccaatctgc accccacgaa cgaaaagatt cttccccgcc ggcgtcgctc tctcatccgt 360

cgacgtctcg ctccttcctt cctctctctc tctctctctc tctctctctc tctctctctc 420

tttctctctc tcagccagca gcaagcatat agcccagcac caccacatgc ccaagggccc 480

tccctctccc tcgtcgtcgg gccaattgag tgtgagagct cgaaaaaacc caggggatcg 540

gccggaccag cagcgagcga gcgcgagctc tgcgtgcgtg tgtgtgtctg cgcgctagat 600

tagatcacct ccatcgtcaa taattgcagg cagatccata tagtctgctg gtggcgagac 660

aaagcaacgg atcgtcgtcg atccggagag cggaaagcgc gcagatcgcg gcggccat 718

<210> 95

<211> 21

<212> RNA

<213> Glycine max

<400> 95

agaaucuuga ugaugcugca u 21

<210> 96

<211> 21

<212> RNA

<213> Glycine max

<400> 96

ggaaucuuga ugaugcugca g 21

<210> 97

<211> 21

<212> RNA

<213> Glycine max

<400> 97

ugaaucuuga ugaugcugca u 21

<210> 98

<211> 21

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence

<400> 98

atgcagcatc atcaagattc t 21

<210> 99

<211> 21

<212> RNA

<213> Glycine max

<400> 99

augcagcauc aucaagauuc u 21

<210> 100

<211> 21

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence

<400> 100

ctgcagcatc atcaagattc c 21

<210> 101

<211> 21

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence

<400> 101

atgcagcatc atcaagattc a 21

<210> 102

<211> 328

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence

<400> 102

ggcagagccg tgcccgtctc atcccctgcc cgtgcaagca gctaggtagg acgatttgag 60

cgtggtgtta ggccgaaccg ctgaaggaag attgctccac tgttgactgc atttgggcta 120

ggtgcatcgg gataaatgta ttgcttatat tcagcaatat aatgttcatc ccgatatgcc 180

tagcccaaat atagtcgata gtggaagaac ggtaacatat gtggtttgca gcaggtgagc 240

aggatgggtg tggatgattg aatatctctg ttcagtgttt tcatcatctg actgaacact 300

gaatcagctt gctgacgtta gaggttag 328

<210> 103

<211> 21

<212> RNA

<213> Glycine max

<400> 103

ugacagaaga gagugagcac a 21

<210> 104

<211> 21

<212> RNA

<213> Glycine max

<400> 104

uugacagaag auagagagca c 21

<210> 105

<211> 21

<212> RNA

<213> Glycine max

<400> 105

ugacagaaga gagugagcac u 21

<210> 106

<211> 21

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence

<400> 106

gtgctcactc tcttctgtca a 21

<210> 107

<211> 21

<212> RNA

<213> Glycine max

<400> 107

gugcucacuc ucuucuguca a 21

<210> 108

<211> 21

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence

<400> 108

tgtgctcact ctcttctgtc a 21

<210> 109

<211> 21

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence

<400> 109

gtgctctctc tcttctgtca a 21

<210> 110

<211> 21

<212> DNA

<213> Artificial sequence

<220>

<223> Artificial sequence

<400> 110

agtgctcact ctcttctgtc a 21

<---

Claims (29)

1. A recombinant DNA construct for modulating floral development in a plant, comprising a first expression cassette and a second expression cassette, wherein the first expression cassette contains a polynucleotide sequence encoding the florigenic FT protein containing an amino acid sequence having at least 90% sequence identity with the sequence, selected from the group consisting of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 and 30, or a functional fragment thereof, operably linked to the first promoter suitable for expression in plants, and the second expression cassette contains a DNA sequence suitable for transcription encoding an RNA molecule containing a targeting sequence that is at least 80% complementary to at least 19 consecutive nucleotides of the polynucleotide sequence of the first expression cassette, and when this specified DNA sequence, one for transcription is operably linked to a second promoter suitable for expression in plants. 2. Recombinant DNA construct according to claim 1, characterized in that the targeting sequence of the RNA molecule is at least 85% complementary to at least 19 consecutive nucleotides of the polynucleotide sequence of the first expression cassette or mRNA encoded by the polynucleotide sequence of the first expression cassette. 3. Recombinant DNA construct according to claim 1, characterized in that the targeting sequence of the RNA molecule is at least 90% complementary to at least 19 consecutive nucleotides of the polynucleotide sequence of the first expression cassette or mRNA encoded by the polynucleotide sequence of the first expression cassette. 4. Recombinant DNA construct according to claim 1, characterized in that the targeting sequence of the RNA molecule is at least 80% complementary to at least 21 consecutive nucleotides of the polynucleotide sequence of the first expression cassette or mRNA encoded by the polynucleotide sequence of the first expression cassette. 5. Recombinant DNA construct according to claim 1, characterized in that said DNA sequence suitable for transcription contains a sequence that is at least 80% complementary to SEQ ID NO: 65, 68 or 69. 6. Recombinant DNA construct according to claim 1, characterized in that the florigenic FT protein additionally contains one or more of the following amino acids: tyrosine or another uncharged polar or non-polar residue at the amino acid position of the florigenic FT protein corresponding to amino acid position 85 in SEQ ID NO : fourteen; leucine or other non-polar residue at the amino acid position of the FT florigenic protein corresponding to amino acid position 128 in SEQ ID NO: 14; and tryptophan or other large non-polar residue at the amino acid position of the FT florigenic protein corresponding to amino acid position 138 of SEQ ID NO: 14. 7. Recombinant DNA construct according to claim 1, characterized in that the florigenic FT protein does not contain one or more of the following amino acids: histidine at the amino acid position corresponding to lysine or arginine at the amino acid position corresponding to position 85 in SEQ ID NO: 14; lysine or arginine at the amino acid position corresponding to position 128 in SEQ ID NO: 14; and serine, aspartic acid, glutamic acid, lysine or arginine at the amino acid position corresponding to position 138 in SEQ ID NO: 14. 8. Recombinant DNA construct according to claim 1, characterized in that said polynucleotide sequence is at least 60% identical to a sequence selected from the group consisting of SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27 and 29. 9. Recombinant DNA construct according to claim 1, characterized in that said first promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO : 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57 , 58, 59, 60, 61, 62, 63 and 64, or a functional part thereof. 10. Recombinant DNA construct according to claim 1, characterized in that the second promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, or a functional part thereof. 11. Recombinant DNA construct according to claim 1, characterized in that the second promoter suitable for expression in plants contains a polynucleotide sequence that is at least 70% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93 and 94, or its functional part. 12. Recombinant DNA construct according to claim 1, characterized in that the first promoter suitable for expression in plants is a vegetative stage promoter, and the second promoter suitable for expression in plants is a late vegetative stage promoter and/or a generative stage promoter . 13. A recombinant DNA construct according to claim 12, characterized in that the first promoter suitable for expression in plants is an early vegetative stage promoter. 14. A recombinant DNA construct according to claim 12, wherein the second promoter suitable for expression in plants is the preferred generative stage promoter. 15. Recombinant DNA construct according to claim 1, characterized in that the first promoter suitable for expression in plants initiates detectable expression of the polynucleotide sequence encoding the florigenic FT protein at an earlier stage of development than that at which the second promoter suitable for expression in plants, initiates detectable expression of said transcribed DNA sequence. 16. Transgenic plant for self-modulation of floral development, containing the insertion of the recombinant DNA construct according to claim 1 in the genome of at least one cell of the transgenic plant. 17. A transgenic plant according to claim 16, characterized in that said transgenic plant is a soybean. 18. A transgenic plant according to claim 17, wherein said transgenic soybean plant produces more pods per node than a control plant that does not contain the recombinant DNA construct. 19. A transgenic plant according to claim 16, characterized in that said transgenic plant produces more flowers per node than a control plant that does not contain said recombinant DNA construct. 20. A transgenic plant according to claim 16, wherein said transgenic plant produces more seeds, pods, pods, fruits, nuts, or beans per node of the transgenic plant than a control plant lacking said recombinant DNA construct. 21. A transgenic plant according to claim 16, characterized in that said transgenic plant blooms earlier than a control plant that does not contain said recombinant DNA construct. 22. A transgenic plant according to claim 16, characterized in that said transgenic plant has more flower clusters per node than a control plant that does not contain said recombinant DNA construct. 23. Part of a transgenic plant containing an insert of a recombinant DNA construct according to claim 1 for self-modulation of floral development in the genome of at least one cell of the transgenic plant part. 24. A transgenic plant part according to claim 23, characterized in that the transgenic plant part is a part of a transgenic soybean plant. 25. The transgenic plant part of claim 24, wherein the transgenic plant part is a soybean plant part that produces more pods per node than a control plant that does not contain the recombinant DNA construct. 26. A transgenic plant part according to claim 23, wherein the transgenic plant part is a part of a plant that produces more flowers per node than a control plant that does not contain said recombinant DNA construct. 27. A transgenic plant part according to claim 23, wherein the transgenic plant part is a plant part that produces more seeds, pods, pods, fruits, nuts, or beans per node of the transgenic plant than a control plant that does not contain said recombinant DNA construct. 28. A transgenic plant part according to claim 23, characterized in that the transgenic plant part is a part of a plant that blooms earlier than a control plant that does not contain said recombinant DNA construct. 29. A transgenic plant part according to claim 23, wherein the transgenic plant part is a part of a plant that has more flower clusters per node than a control plant that does not contain said recombinant DNA construct.

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