RU2712916C1 - Method of anesthesia in balb/c nude mice in surgical interventions - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to experimental oncology, and is intended for anesthesia in Balb/c Nude mice in surgical interventions. Method of anesthesia in Balb/c Nude mice in surgical interventions involves xylazine premedication intramuscularly and anesthesia with the help of "Zoletil 100", which is administered intramuscularly. A preparation of xylazine with concentration of 20 mg/ml is introduced in dose of 1.5 ml/kg. In 15 minutes after pre-medication preparation "Zoletil 100" is introduced in the form of aqueous solution with concentration of 22.57 mg/ml in dose of 67.5 mg/kg of body weight of Balb/c Nude mice.

EFFECT: use of the invention provides humane treatment of Balb/c Nude mice in surgical interventions due to optimal depth of anesthesia and minimal toxic effect on the body of mice.

1 cl, 1 ex

Description

The invention relates to medicine, namely to experimental oncology, and relates to a method for anesthesia in Balb / c Nude mice during surgical interventions.

Animal experiments, controlled by the Rules of Good Laboratory Practice and the paragraphs of the Helsinki Declaration, are an important component of a biomedical study, without which it is impossible to understand the nature and pathophysiology of many diseases and develop appropriate treatment strategies (see Use of Laboratory Animals in Biomedical and Behavioral Research // National Academy of Sciences. - P. 1-2). When using small rodents (mice and rats) in an experiment, adequate anesthesia and the use of analgesics to relieve pain during animal recovery are necessary to minimize the pain shock that causes animal death during surgery (see Andersen ML, Winter LMF Animal models in biological and biomedical research - experimental and ethical concerns // An. Acad. Bras. Scienc. - 2017). The choice of anesthesia is determined by key factors: breed, age, gender, weight, type and duration of surgical procedures (see J.G. Vandenbergh Use of House Mice in Biomedical Research // ILAR journal. - 2000.). Failure to comply with these points leads to serious consequences, causing ulceration on the mucous membranes, expansion of the veins of the lower third of the esophagus, spleen, intestines, kidneys, blood clots in large vessels of the abdominal cavity (see A.V. Smirnova, L.D. Lagutina, I. E. Trubitsyna, OS Vasnev, OB Yanova Features of anesthesia in rats during abdominal operations // Experimental and clinical gastroenterology. - 2012. - No. 5. - P. 62-65). Improving the dosage of drugs used in anesthesia, and the choice in favor of combined anesthesia will reduce the risk of painful death of animals and minimize postoperative complications.

A known method of anesthesia in experimental animals using drugs based on Tribromeyanol (RE Meyer, RE Fish A review of tribromoethanol anesthesia for production of genetically engineered mice and rats // Lab Animal. - 2005. - No. 34. - P. 47-52 ) This method provides muscle relaxation and adequate anesthesia for 15-30 minutes. The disadvantage of this method is the occurrence of foci of necrotization of muscle tissue. In addition, an overdose of the drug causes peritonitis and excruciating death of rodents, which contradicts the international rules for surgical interventions in animals.

A known method of anesthesia of mice with diethyl ether using an impregnated cotton swab by inhalation to ensure long-term anesthesia (K. Otto, A. Thaden The Laboratory Mouse // Chapter 5.4 - Anaesthesia, Analgesia and Euthanasia. - 2012. - P. 739-759) . The main disadvantage of this method is the high toxicity and carcinogenicity of diethyl ether for humans.

A known method of injection anesthesia, involving oral, intravenous and intramuscular administration of barbiturates (Thiopental, Pentobarbital) (S. Gargiulo Mice Anesthesia, Analgesia, and Care, Part I: Anesthetic Considerations in Preclinical Research // ILAR journal. - 2012). This method provides the depth of anesthesia necessary for long operations, since the drugs used are derivatives of γ-aminobutyric acid, which inhibit the effects of norepinephrine and glutamate. However, when using this method, numerous liver tissue damage occurs due to high hepatotoxicity, leading to suffering in laboratory animals and subsequent death. Another drawback is the inaccessibility of Thiopental and Pentobarbital due to the limited circulation of barbiturate preparations worldwide.

A known method of anesthesia in rats by premedication with Xylazine (Interchemie werken "De Adelaar" BV, Netherlands) at a dose of 0.05 ml / kg of body weight intramuscularly and the introduction of the drug "Zoetil 50" (Virbac, France) at a dose of 0.5 mg / kg body weight 10 minutes after premedication (Pat. No. 2466753 of the Russian Federation. The method of anesthesia in rats with a multi-stage experiment when modeling endometriosis. / Adamyan L.V. Publ. 06/03/2011). The described method provides muscle relaxation, sedation and dissociative anesthesia, since Xylazine hydrochloride is an antagonist of central alpha-2-adrenergic receptors, and Tiletamine hydrochloride is a competitive antagonist that inhibits the connection of the N-methyl-D-aspartate receptor, which provides signal transmission between the brain and the neuron between the neurons and the spin , with glutamate (D.V. Kadomtsev, E.A. Pasechnikova, V.G. Golubev Zoetil-xylazine anesthesia in experiments in rats // International Journal of Applied and Basic Research. - 2015). The disadvantage of this method is the occurrence of postoperative seizures and increased salivation due to numerous injuries of the cortex of the cingulate gyrus and retrosplenal cortex in rats, leading to painful death of animals.

The technical result of the invention is the development of a method for anesthesia in Balb / c Nude mice during surgical interventions, providing an optimal depth of anesthesia and minimal toxic effect on the body of mice.

The technical result of the invention is achieved by the fact that anesthesia is performed in Balb / c Nude mice during surgical interventions, including premedication with xylazine intramuscularly, anesthesia with the drug Zoletil 100, which is administered intramuscularly, characterized in that the xylazine preparation has a concentration of 20 mg / ml administered at a dose of 1.5 ml / kg, 15 minutes after premedication, the drug “Zoetil 100” was administered as an aqueous solution at a concentration of 22.57 mg / ml at a dose of 67.5 mg / kg of body weight of Balb / c Nude mice.

The method is as follows.

Premedication is carried out using xylazine at a concentration of 20 mg / ml, which is administered intramuscularly at a dose of 1.5 ml / kg. Fifteen minutes after premedication, Zoetil 100 is administered intramuscularly as an aqueous solution at a concentration of 22.57 mg / ml at a dose of 67.5 mg / kg body weight of Balb / c Nude mice.

In this way, 210 mice of the Balb / c Nude line were anesthetized.

Example.

A male Balb / c Nude mouse weighing 22 g was injected intramuscularly with xylazine (Xyla preparation) at a dose of 0.03 ml. After 15 minutes, the drug “Zoletil 100” was administered intramuscularly in the form of an aqueous solution with a concentration of 22.57 mg / ml at a dose of 0.07 ml (1.485 mg of the dry preparation “Zoletil 100”).

The technical and economic effectiveness of this method lies in the fact that it allows for the humane treatment of Balb / c Nude mice during surgical interventions due to the use of anesthesia based on two drugs: xylazine and Zoleta 100.

Claims (1)

A method of anesthesia in Balb / c Nude mice during surgical interventions, including premedication with xylazine intramuscularly, anesthesia with the drug “Zoletil 100”, which is administered intramuscularly, characterized in that the drug xylazine concentration of 20 mg / ml is administered at a dose of 1.5 ml / kg, 15 minutes after premedication, the drug “Zoetil 100” is administered in the form of an aqueous solution with a concentration of 22.57 mg / ml at a dose of 67.5 mg / kg of body weight of Balb / c Nude mice.