RU2709766C1 - Device for production of sperm of reindeers in field conditions - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: invention refers to veterinary obstetrics, particularly to a device for sperm collection in reindeers in field conditions. Device is characterized by that it consists of a thermo-vessel with a foamed polysterol cover, a sterile test tube for sperm collection and a funnel made of neutral non-toxic semen material. Vessel is made in the form of thermal moker with volume of 250 ml, filled with 2/3 water with temperature of 36–38°C, and a sterile test tube for collecting semen is inserted into an opening in the thermo-vessel cover.

EFFECT: use of the invention enables preserving the biological usefulness of sperm when produced in field conditions.

1 cl, 1 tbl, 2 dwg

Description

The invention relates to reindeer herding. It can be used in the field of conservation of Arctic genetic resources and artificial insemination of reindeer. The device is designed to produce full reindeer sperm (Rangifer tarandus) using electro-ejaculation.

Electro-ejaculation is recommended for collecting sperm from untamed or bred animals with insufficient domestication. When working with an electric ejaculator, the operator enters the ejaculator into the rectum to a depth of 8-10 cm and delivers current with short pulses (Mkrtchyan M.E., 1971, Mkrtchyan M.E., Deryazhentsev V.I., 1973). After an erection and ejaculation, the sperm enters a sterile vessel, which enters the laboratory for further research. It takes time to get sperm and deliver it to the laboratory, during which the ejaculate is in the open air, which negatively affects the quality of the sample. Reindeer sperm is collected only during the rutting season, which occurs in the cold months (late September - early November). Low temperature has a negative effect on the quality of the ejaculate. Therefore, there was a need to develop a device to preserve the biological usefulness of reindeer sperm at the time of receipt.

The proposed device is designed to obtain full sperm in the field (in the absence of warm pens). The described device (Fig. 1) consists of a thermal vessel (1) (thermocup) with a volume of 250 ml with a foam polystyrene cap (2). Water with a temperature in the range of 36-38 ° C is poured into the thermo vessel (1). A sterile tube (3) for collecting ejaculate together with a funnel (4) from a neutral (non-toxic to semen) material is inserted into the prepared hole in the lid. A sufficient volume of warm water allows you to keep the temperature of the seed in a test tube avoiding cold shock, and a foam cover fixes the funnel and avoids heat loss. The thermocup handle makes it easier for the operator to work with the animal.

Principle of operation. The animal is safely fixed before electro-ejaculation. Thermal vessel (1) is filled 2/3 with water 36-38 ° C and tightly closed with a lid (2) of expanded polystyrene foam. A sterile tube (3) for collecting ejaculate together with a funnel (4) from a neutral (non-toxic to semen) material is inserted into the prepared hole in the lid. The operator enters the ejaculator (5) into the rectum to a depth of 8-10 cm and delivers 1 current with short pulses. During electro-ejaculation, the operator, holding the device by the handle, brings the funnel (4) under the erect penis (Fig. 2). The ejaculate is injected through a funnel (4) into a test tube (3) heated to the required temperature. If necessary, the tube is closed with a screw cap and quickly changed by the operator to obtain a different fraction of sperm.

To verify the efficiency of use of the device, tests were conducted. Table 1 shows the results. The main function of the sperm is the delivery of nuclear (genetic) material, concentrated in its head, to the egg. This function is provided due to the ability of sperm to translate. The lack of mobility or a significant violation of the nature of the movement of sperm shows the unsuitability of sperm for artificial insemination. The presence of sperm movement is not an indicator to assess the fertilizing ability of sperm. Often, sperm with a sufficiently high mobility do not have a fertilizing ability. Motility and speed are indicators that characterize the state of the kinetic apparatus of sperm. However, as is known, sperm penetration into the ovum is associated with an acrosomal reaction and therefore depends on the state of the acrosomal sperm apparatus. It is clearly seen that, in contrast to obtaining sperm without a device in a regular sperm receiver, the use of the device allows you to maintain high quality sperm. Compared to using a conventional sperm receiver, the overall mobility of the cells has not changed, and the progressive motility has increased - which indicates a decrease in cold shock. Also, the number of cells without damage increased, and the number of injuries in the tail, as well as the number of injuries by acros, decreased. It is seen that the object of the invention has an advantage over a conventional sperm receiver.

Using this device allows you to not only get full sperm, but also save it until laboratory testing without loss of quality.

Sources of information

1. Mrktchyan M.E. Copyright certificate N 385562 "Methods of obtaining seed from untamed animals." M., 1971.

2. Mkrtchyan M.E., Deryazhentsev V.I. On the issue of taking semen from choirs by electro-ejaculation method. Collection of scientific works of the Murmansk reindeer herding experimental station, 1973. - Vol. 2. - S. 31-33.

3. Goncharov, V.V. Improving the technology of rutting and calving of reindeer Evenki breed in the taiga zone. Abstract. dis. for a job. scientist Art. Cand. S.-kh. Sciences, - 2003, Novosibirsk

4. Goncharov V.V. Nikitkina E.V. On the issue of artificial insemination and cryopreservation of reindeer sperm // Genetics and animal breeding. - 2016. - No. 2. - S. 3-7.

Claims (1)

A device for collecting sperm from reindeer in the field, characterized in that it consists of a thermal vessel with a foam polystyrene cap, a sterile sperm collection tube and funnel made of neutral material non-toxic to sperm, the vessel made in the form of a thermomug with a volume of 250 ml, filled at 2/3 with water at a temperature of 36-38 ° C, and a sterile sperm collection tube is inserted into the hole in the lid of the thermos vessel.

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