RU2697840C1 - Method of producing nanocapsules of reishi dry extract (ganoderma lucichum karst.) - Google Patents

Abstract

FIELD: nanotechnologies.

SUBSTANCE: invention relates to nanotechnology, in particular to a method of producing nanocapsules, and describes a method of producing nanocapsules of dry extract of Reishi in a shell of sodium alginate. Method is characterized by that dry extract of Reishi is added to a suspension of sodium alginate in methanol in presence of 0.01 g of glycerol ester with one or two molecules of food fatty acids and one or two molecules of citric acid as a surfactant while stirring 900 rev/min, then ethyl acetate is poured, the obtained suspension of nanocapsules is filtered and dried at room temperature, wherein the weight ratio of core to shell is 1:1, 1:2 or 1:3.

EFFECT: method ensures the nanocapsules producing process simplification and acceleration, reduction in losses during the nanocapsules production, and can be used in the pharmaceutical and food industries.

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Description

The invention relates to the field of nanotechnology, medicine, pharmacology, cosmetic and food industries.

Previously known methods for producing microcapsules.

In US Pat. 2173140 IPC A61K 009/50, A61K 009/127 Russian Federation published September 10, 2001. A method for producing silicon organolipid microcapsules using a rotary-cavitation installation with high shear forces and powerful sonar acoustic and ultrasonic dispersion ranges is proposed.

The disadvantage of this method is the use of special equipment - a rotary cavitation unit, which has an ultrasonic effect, which affects the formation of microcapsules and can cause adverse reactions due to the fact that ultrasound destructively affects polymers of a protein nature, therefore, the proposed method is applicable when work with polymers of synthetic origin

In US Pat. 2359662 IPC A61K 009/56, A61J 003/07, B01J 013/02, A23L 001/00 published 06/27/2009 The Russian Federation proposed a method for producing microcapsules of sodium chloride using spray cooling in a Niro spray cooling tower under the following conditions: inlet air temperature 10 ° C, outlet air temperature 28 ° C, the rotation speed of the spray drum 10,000 rpm. The microcapsules of the invention have improved stability and provide controlled and / or prolonged release of the active ingredient.

The disadvantages of the proposed method are the duration of the process and the use of special equipment, a set of certain conditions (air temperature at the inlet 10 ° C, air temperature at the outlet 28 ° C, rotation speed of the spray drum 10,000 rpm).

The closest method is the method proposed in US Pat. 2134967 IPC A01N 53/00, A01N 25/28 published on 08.27.1999 Russian Federation (1999). A solution of a mixture of natural lipids and a pyrethroid insecticide in a weight ratio of 2-4: 1 in an organic solvent is dispersed in water, which simplifies the microencapsulation method.

The disadvantage of this method is dispersion in an aqueous medium, which makes the proposed method inapplicable for producing microcapsules of water-soluble preparations in water-soluble polymers.

The technical task is to simplify and accelerate the process of obtaining nanocapsules, reduce losses in obtaining nanocapsules (increase in yield by mass).

The solution of the technical problem is achieved by the method of producing nanocapsules, characterized in that sodium alginate is used as the shell of the nanocapsules, and a dry reishi extract is used as the core when nanocapsules are prepared by the non-solvent precipitation method using ethyl acetate as a precipitant.

A distinctive feature of the proposed method is the preparation of nanocapsules by non-solvent precipitation using ethyl acetate as a precipitant, as well as the use of sodium alginate as a particle shell and dry reishi extract as a core.

The result of the proposed method are obtaining nanocapsules of dry extract of Reishi.

EXAMPLE 1 Obtaining nanocapsules of dry extract of Reishi, the ratio of the core: shell 1: 3

1.5 g of a dry extract of Reishi is added to a suspension of 4.5 g of sodium alginate in methanol in the presence of 0.01 g of the preparation E472c (glycerol ester with one or two molecules of food fatty acids and one or two molecules of citric acid, with citric acid, as tribasic, it can be esterified with other glycerides and as an acid with other fatty acids. Free acid groups can be neutralized with sodium) as a surfactant with stirring at 900 rpm. Then poured 8 ml of ethyl acetate. The resulting suspension is filtered and dried at room temperature.

Received 6 g of nanocapsule powder. The yield was 100%.

EXAMPLE 2 Obtaining nanocapsules of dry extract of Reishi, the ratio of the core: shell 1: 1

2 g of dry extract of rosemary is added to a suspension of 2 g of sodium alginate in methanol in the presence of 0.01 g of the preparation E472c as a surfactant with stirring at 900 rpm. Then poured 8 ml of ethyl acetate. The resulting suspension is filtered and dried at room temperature.

Received 4 g of nanocapsule powder. The yield was 100%.

Example 3 Preparation of Nanocapsules of Reishi Dry Extract, Core: Shell Ratio 1: 2

2 g of dry extract of rosemary is added to a suspension of 4 g of sodium alginate in methanol in the presence of 0.01 g of the preparation E472c as a surfactant with stirring at 900 rpm. Then poured 8 ml of ethyl acetate. The resulting suspension is filtered off and dried at room temperature.

Received 6 g of nanocapsule powder. The yield was 100%.

Claims (1)

A method of producing nanocapsules of a dry extract of Reishi, characterized in that the dry extract of Reishi is added to a suspension of sodium alginate in methanol in the presence of 0.01 g of glycerol ester with one or two molecules of food fatty acids and one or two molecules of citric acid as a surface-active substances with stirring 900 rpm, then poured ethyl acetate, the resulting suspension of nanocapsules is filtered off and dried at room temperature, while the mass ratio of the core: shell is 1: 1, 1: 2 or 1: 3.