RU2693410C1 - Composition with a supramolecular structure of a colloidal mixture of complex compounds of nanostructure particles of silver or hydrosol of silver cations in an aqueous or in an aqueous organic solution, having antimicrobial and antitoxic action (versions), and a method for production thereof - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: group of inventions relates to the field of medicine and is intended for production and application in the food, perfume and pharmaceutical industry of the composition possessing antimicrobial and antitoxic action. Composition with supramolecular structure of colloidal mixture of complex compounds of nanostructured silver particles includes active metals in form of binary mixture of colloidal solution of nanostructured silver particles with particle size of 2–100 nm and colloidal solution of silver cations; silver cation restorer selected from citric acid and ascorbic acid; stabilizer of binary mixture of colloidal solution of nanostructured silver particles and silver cations in aqueous dispersion in form of polyvinyl alcohol; distilled water or deionized water. Colloidal solution of silver cations contains a hydrosol of silver cations in an aqueous or aqueous organic solution. Composition includes complex compounds of an emulsifier in form of sodium dioctyl sulphosuccinate surfactants, a dispersing agent in form of a heterocyclic compound of polyvinylpyrrolidone, a thickener in form of glycerol, complexing biologically active substances in the form of mycelium "OVODORIN" mycolic extract and a carrier of a colloidal mixture of propylene glycol complex compounds. Components are used in claimed amounts. Method of producing said composition is also provided.

EFFECT: use of the group of inventions enables to obtain a composition with a supramolecular structure synthesized based on complex compounds of nanostructured particles of silver and/or hydrosol of silver cations with formation of stabilized synthesized structures, wherein the composition has antimicrobial and antitoxic action.

2 cl, 6 tbl, 7 ex

Description

The invention relates to the field of obtaining compositions of complex compounds of nanostructured silver particles and hydrosol of silver cations with sizes of structural formations of the order of nanometers and less in the presence of complexing polymeric and biological substances prepared during the formation of a solution containing, inter alia, supramolecular structures with antimicrobial and antitoxic effects. The resulting compositions can be used in the food and perfumery and pharmaceutical industries for the production of dietary supplements, cosmetics, medical products, medicines, protective and disinfectants in the form of solutions, gels or films with specific properties.

In general, the composition of complex compounds of nanostructured particles of silver and silver hydrosol cations is a supramolecular complex formed as a result of the association of particles of the active substance and auxiliary substances held together by intermolecular forces.

It is known that supramolecular systems are polymolecular structures formed as a result of the association of particles of small size, such as molecules, oligomers, clusters, held together by intermolecular forces [RF patents 2423384, No.2562113; Jean-Marie Len. Supramolecular chemistry. Concepts and perspectives. Science, Novosibirsk, 1998. - 334 p.].

The use of supramolecular structures for the development of compositions containing nanostructured silver particles or silver cations synthesized from them in the presence of complexing polymeric and biological substances prepared in the process of solution formation opens up new prospects for obtaining a variety of innovative tools and materials widely used in food, cosmetic and medical industries for the production on their basis of new safe, antiviral, antimicrobial and anti-toxic effects. The drugs that can replace a wide range of a number of chemically synthesized antibiotics in the shortest possible time.

For example, a new class of supramolecular structures synthesized on the basis of L-cysteine and silver nitrate has already been opened [RF Patents 2423384, No. 2562113]. These structures are constructed from intersecting chains of nanoscale, positively charged fractal clusters [Smirnov B.М. Physics of fractal clusters. M .: Nauka, 1991, - 136 pp.] And represent a combination of fragments of a three-dimensional grid, distributed in an aqueous or aqueous-organic medium [RF Patent №2423384]. It has been established [RF Patent No. 2562113] that these structures are able to effectively interact with negatively charged groups present on the cell surface of the microorganism, which gives the L-cysteine-silver solution the properties of a cationic antiseptic.

It is known [T. Franklin, J. Snow. Biochemistry of antimicrobial action; Translation from ang. M.A. Panov. M .: Mir, 1984. - 238 p. with il.], that cationic antiseptics include chemical compounds of different nature, having in their structure strongly basic functional groups, among which free or substituted amino groups, imino group, guanidine group are important. The main groups of these antiseptics in a weakly acidic environment acquire a positive charge and therefore they interact with the negatively charged cytoplasmic membranes of bacteria, disrupting their functioning [Patent No. 2562113]. Well known [T. Franklin, J. Snow. Biochemistry of antimicrobial action; Translation from ang. M.A. Panov. M .: Mir, 1984. - 238 p. with il.] cationic antiseptic - chlorhexidine, having in its composition two strongly basic diganidine groups.

The analysis of known publications [Muzzarell R., Biagini G., Pugnaloni A., Reconstruction of parodontal tissue with chitosan // Biomaterials, 1989, v. 10, No. 9, p. 598-603; Patents of the Russian Federation 2423384, No. 2562113] showed that available information sources describe compositions with the supramolecular structure of a colloidal mixture with various compounds, namely low-molecular substances, clusters of nitrates or silver citrates, non-ionic polymers, negatively charged polyelectrolytes.

However, in the development of known compositions, the bioactive and complexing properties of chitosan alone are related to silver nitrates or silver citrates, which, as is well known [RF patents №2601737 dated 09.06.2015, №2609176 dated 09.06.2015], have a number of disadvantages , limiting the effectiveness and scope of their application.

In addition, a composition containing chitosan (0.1%), ascorbic acid (0.165%) and sodium ascorbate used in dental surgery for healing defects of the paradontal tissue is known [Muzzarell R., Biagini G., Pugnaloni A., Reconstruction of parodontal tissue with chitosan // Biomaterials, 1989, v. 10, No. 9, p. 598-603.]. Antimicrobial compositions based on low molecular weight chitosan have been developed, including thickeners such as polyvinyl alcohol, glycerin, polyethylene glycol, polyvinylpyrrolidone [- Access mode: http://www.findpatent.ru/patent/256/2562113.html © FindPatent.ru - patent search, 2012-2016]. On the property of chitosan to form polyelectrolyte complexes, its application in medicine and biotechnology for the development of bioconstructive materials, membrane films, sorbents is based [M.A. Krajukhina, N.A. Samoilova, I.Ya. Yamkov, Advances of Chemistry, 77, 854 (2008)], as well as antimicrobial compositions, for example, with an antibiotic of a polypeptide type produced by a bacterium of the genus Streptococcus, nisinum [Kozlov A.V. Development of an antimicrobial composition based on nisin and chitosan for use in the technology of natural semi-finished meat, smoked meats and ham. Thesis - M .; 2010. - 206 s. with il.]. Also known are antimicrobial compositions based on microgranules of chitosan gel and cluster particles of silver of small sizes [Vasilenko SK, Burmistrov VA, Simonova OG, Belyaev MD. The power of the sea and silver. Microgranulated chitosan Argo VASNA gel, Vector-Invest Research and Production Center, Novosibirsk, 2003].

The analysis of the chemical composition of a known composition with the supramolecular structure of a colloidal mixture of complex compounds of nanoscale particles of silver or silver ions in aqueous or in aqueous-organic solution and methods for their preparation [RF patents 2423384, No. 2562113] showed that in these and other known technical solutions For the formation of supramolecular structures from among silver-containing chemicals, the known silver salts in the form of silver nitrate or citrate are used.

Despite the fact that such preparations created by this type, for example, based on colloidal nitrate or silver citrate, have been widely used in medicine as external antiseptics for more than 100 years, they are limited in their use to date due to a number of inherent for there are disadvantages [Patents of the Russian Federation No. 2601737 of 06/09/2015, No. 2609176 of 06/09/2015]. For this reason, throughout this time, the development of compositions and methods for their preparation continues. For example, stabilizers are introduced into the known colloidal silver preparations (collargol and protargol) obtained by a chemical method to increase the biological activity. Protein polymers casein and gelatin serve as a stabilizer of highly dispersed silver particles [Mashkovsky MD Medicines. In 2 t. Tom. 2. 14th ed., Revised., Rev. and add. - M .: New Wave, 2002. 608 p.].

However, along with a wide range of positive results obtained from the use of this kind of drugs, which are based on both nanostructured particles of metals in aqueous and organic dispersions and metal ions in aqueous dispersions, widely published in Russia and abroad, the technology for their preparation remains still not perfect for producing pure, impurity-free stabilized nanostructured particles of silver or silver cations, currently being successfully used in various fields of science and technology, of the inherent drawbacks of a number of final product, reduce the efficiency or limited their use as targeted agents while possessing antimicrobial and antitoxic effect.

We have found that both when obtaining colloidal solutions of nanostructured metal particles in organic and aqueous dispersions by all the above-mentioned physical and chemical methods, and in obtaining colloidal solutions of silver ions from salts or silver crystals in the presence of reducing agents, 100% preparation or nanostructured silver particles, or silver ions in pure, non-admixed colloidal solutions due to technological improvement of the existing known technologist th [RF Patent №2601737 of 09.06.2015 city, №2609176 from 09.06.2015 g].

As a rule, in the production of colloidal solutions of nanostructured silver particles one way or another of the known methods, at the end of the technological process, a mixture of nanostructured metal particles with a part of unrestored nanostructured metal particles as the remaining part of metal salts, atoms with zero valence or metal ions, impurities chloride, nitrate and sulfate ions [RF patents №2601737 from 06.06.2015, №2609176 from 09.06.2015].

Similarly, in the production of metal ions in the presence of organic, or carboxylic, or other acids by known methods, it is also difficult to achieve pure monodisperse colloidal solutions of only metal ions, without admixtures of nanostructured metal particles with a size of 2 nm or more, nanostructured particles of metal oxides and nanostructured hydroxide particles metals, as well as impurities chloride, nitrate and sulfate ions. The interaction of these impurities with the formed nanostructured particles or active metal ions leads further to the low stability of the produced colloidal solutions of nanostructured metal particles in organic or aqueous dispersions, or colloidal solutions of active metal ions in the aqueous dispersion. This disadvantage is explained by the reasons for the formation in solutions of metal ions with impurities of low active insoluble inorganic and organic toxic compounds [RF patents №2601737 of 06.06.2015, №2609176 of 09.06.2015].

Physicochemical processes occurring in such colloidal solutions lead to unstable, time-varying concentrations of active metals in colloidal solutions, their shape and nano-dimension, the formation of insoluble inorganic and organic toxic compounds, which simultaneously leads to a decrease in bactericidal activity and an increase in the toxicity of the drugs used.

The currently developed methods for stabilizing colloidal solutions to reduce or eliminate the above drawbacks are also not perfect and do not remove the current problem of improving the quality of obtaining a binary colloidal mixture of nanostructured silver particles and silver ions in the stabilizer.

To substantiate the essence of the proposed technical solution, identify the totality of distinctive features and prove the compliance of the proposed technical solution with the novelty criterion of the invention, except for the technical solutions discussed above, technical solutions adopted by the authors for analogues and prototypes with an indication of their shortcomings are discussed below.

As a result of the analysis of the state of question regarding the development of compositions with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or aqueous-organic solution, which has an antimicrobial and anti-toxic effect, and nanostructured particles can be widely used today. metals and their derivatives in non-polar solvents and aqueous dispersions [Pomogailo AF Polymermobilized nanoscale and cluster particles. - Successes of chemistry, 1997, t. 66, No. 8, - p. 750; Revina A.A., Egorova E.M. Radiation-chemical nanostructured technology for the synthesis of stable metal and bimetallic clusters. Abstracts of the international conference "Advanced technologies on the threshold of the XXI century", ICAT 98, M., 1998, part II, - p. 411; Pilenie M., et al. Nanostructured particles in colloidal systems. - Langmuir, 1997, vol. 13, - p. 3266; Sergeev G.B. Nanochemistry: study guide / G. B. Sergeev. - 3rd ed. - M .: KDU, 2009. - 336 pp., Il .; Rambidi N.G. Physical and chemical bases of nanotechnology / N.G. Rambidi, A.V. Berezkin. - M .: FIZMAT LIT, 2008. - 456 p .; Suzdalev I.P. Nanotechnology: physical chemistry of nanoclusters, nanostructures and nanomaterials / I.P. Suzdalev. - M .: Kom. Book, 2006. - 597 p.].

It is known [Electronic Resource / Universal antibacterial agent for disinfection based on silver and copper: a superconcentrate SumerSil ^® // - Access mode: http://organic-silver.com/, or organic-silver.com/index/...kopiya], that nanostructured metallic particles are obtained by physical and chemical methods, incl. photochemical, radiation-chemical, electrochemical, biochemical methods Particles of a substance in the range of nanometer size 2-100 nm change their chemical, physical and biological properties, the parameters of which have important practical importance.

Currently, in connection with the development of the antibiotic-resistance of microbes, there is again an increasing interest in the development and creation of various kinds of colloidal silver preparations using silver particles, inclusive, in the form of colloidal solutions of silver ions, copper, gold and other active metals or their binary compounds, for example, silver and copper ions. [Electronic Resource / Universal antibacterial agent for disinfection based on silver and copper: superconcentrate SumerSil ^® // - Access Mode: http://organic-silver.com/, or organic-silver.com/index/...kopiya].

The use of characteristic features of substances with particle sizes less than 0.1 nanometers creates additional, completely new possibilities for creating technological methods and tools related to improving the development of compositions with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or silver cations. a solution with antimicrobial and anti-toxic effects and methods for their preparation, with the aim of their further use in materials chemistry, physics, biology, medicine and many other areas of science and technology.

Taking into account the experience of research conducted in this direction, in our opinion, one of the main areas of modern nanotechnology is the synthesis of nanostructured metallic particles, which is based on the recovery of metal ions to atoms with the subsequent aggregation of atoms and ions to form nanostructured metal particles, without the known disadvantages of these structures, manifestable nanostructured formations obtained on the basis of nitrates or silver citrates [RF patents №2394668 of 12.19.2008, №2601737 of 09.06.2015, №2609176 from June 9, 2015].

The beginning of the above mentioned methods served as a biochemical method for producing nanostructured metal particles developed and published in 1989 [Robinson B. et al. Synthesis and release of microparticles in the reverse micelle system: In the collection “Structure and reactivity in reverse micelles”, edited by Pilaney M. Tokyo , 1989, p. 198].

However, despite the effectiveness of this method of obtaining nanostructured metal particles, it has a number of disadvantages, which primarily include:

- low stability of nanostructured metal particles obtained using many of the developed methods;

- the complexity of the technological production and practical application of nanostructured metal particles;

- relatively low speed of formation of nanostructured metal particles, requiring a significant consumption of reducing agent, which increases the cost of manufacturing nanostructured metal particles;

- An excess of reducing agent and various reaction products may be present in the reaction system.

Among the known methods for producing nanostructured metal particles as promising methods for producing nanostructured metal particles described in [Patents of the Russian Federation: No. 2147487, 7 V22, F 9/24, publ. 2000; No. 2202400, 7 B01D 39/00, B01J 20/20, publ. 2003; No. 2341291 A61L 2/16 (2006.01), A01N 65/00 (2006.01), A61K 33/38 (2006.01), A61K 31/79 (2006.01), publ. December 20, 2008; No. 2394668 of December 19, 2008, publ. July 20, 2010], taken as analogues in the process of composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or silver cations in an aqueous or aqueous-organic solution. The number of such methods for producing nanostructured silver particles is primarily attributed to:

a) A method for producing nanostructured metallic particles by reducing metal ions in the reverse micellar dispersion of a reducing agent based on a solution of a surfactant in a non-polar solvent [41. Patent of the Russian Federation No. 2147487, 7 V22, F 9/24, publ. 2000.];

b) A method for producing an aqueous dispersion of nanostructured metal particles obtained from their reverse micellar solution [RF Patent No. 2202400, 7 B01D 39/00, B01J 20/20, publ. 2003.].

c) A bactericidal solution and method for preparing it [Patent RU 2341291 A61L 2/16 (2006.01), A01N 65/00 (2006.01), A61K 33/38 (2006.01), A61K 31/79 (2006.01), publ. December 20, 2008].

Known inventions are related to the production of dispersions of nanostructured metal particles in water with bactericidal properties used in various fields of medicine, pharmacology, industry and ecology.

At the same time, we found that when implementing the above-mentioned known methods for producing nanostructured metal particles by reducing metal ions in the reverse-molecular dispersion of a reducing agent in volumes greater than 0.1 l, the process of preparing a micellar solution of reducing agent at room temperature (20-25 ° C) is technologically sufficient time-consuming and requires large energy costs for its dissolution in a non-polar solvent, accompanied by constant mixing of the mixture and takes as Awilo from 3 to 5 days, which leads to high cost material and energy costs, despite the fact that eventually leads to the production of nanostructured metal particles with a relatively small concentration of a non-polar solvent (from 2⋅10 ^-4 g ion / l to 3⋅10 ^-3 g-ion / l).

When implementing the known methods of obtaining a water dispersion of nanostructured metal particles [RF Patent No. 2147487, 7 V22, F 9/24, publ. 2000; RF patent №2202400, 7 B01D 39/00, B01J 20/20, publ. 2003; Patent RU 2341291 A61L 2/16 (2006.01), A01N 65/00 (2006.01), A61K 33/38 (2006.01), A61K 31/79 (2006.01), publ. 20.12.2008.], Obtained from their reverse micellar solution after separating the mixture of aqueous dispersion from non-polar solvent in a mixture volume of more than 1 l, further purification of the aqueous dispersion of nanostructured metal particles from an excess of non-polar solvent and surfactant by known methods is time-consuming process and takes As a rule, 3-5 days, which economically leads to large energy and time costs, low productivity of the finished product and increases the level of fire and explosion pasnosti production.

Obtaining a water dispersion of nanostructured metal particles by centrifuging a two-phase system of reverse-cellral solution of nanostructured metal particles and water and separating the lower aqueous phase containing nanostructured metal particles is unacceptable in practice with even small (more than 1 l) volumes of obtaining an aqueous solution of nanostructured metal particles.

From among the known methods of obtaining nanostructured metal particles analyzed by us, the most effective way, which lacks the above listed disadvantages for the indicated essential features, is the Method of obtaining nanostructured metal particles developed in the Russian Federation in 2008 [Patent for invention No. 2394668 dated December 19, 2008 obtain nanostructured metallic particles. Published 07/20/2010, Byul. No.20.], The technical solution of which was taken as the basis for the development of two new methods of obtaining: the composition of a binary colloidal mixture of nanostructured silver particles and silver ions in a stabilizer and a monodisperse colloidal solution of silver ions with antimicrobial and anti-toxic effects and the way they are produced.

The result of the new technologies that have been developed is that the newly created environmentally friendly and safe technologies for producing nanostructured particles of silver and silver cations, patented in the Russian Federation, include:

- patent for invention №2601757 from 09.06.2015. Composition of a binary colloidal mixture of nanostructured particles of silver and silver ions in a stabilizer, which has an antimicrobial and antitoxic effect (versions) and a method for its preparation. Published on 10.11.2016, Bull. No. 31;

- patent for invention No. 2609176 dated June 09, 2015. Monodisperse colloidal solution of silver ions having antimicrobial and anti-toxic effects (versions) and a method for producing them. Published 30.01.2017, Bull. №1.

These technical solutions were taken as prototypes and formed the basis for the development of the claimed invention "Composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured particles of silver or hydrosol of silver cations in an aqueous or aqueous-organic solution, possessing antimicrobial and antitoxic action (options) and method receive it. "

"The composition of a binary colloidal mixture of nanostructured particles of silver and silver ions in a stabilizer, which has an antimicrobial and anti-toxic effect (versions) and a method for producing it" [invention patent No. 2601757 dated 09.06.2015. Publ. 11/10/2016, Bull. No. 31] relates to methods for producing nanostructured particles of silver and silver ions with sizes of structural formations of the order of nanometers and less in the presence of polymer stabilizers with antimicrobial and anti-toxic effects and used in various fields of science, medicine, pharmacology, industry, agriculture and ecology.

The technical result of this invention is to obtain environmentally safe stable binary colloidal mixtures of nanostructured particles of silver and silver ions in the presence of stabilizers with a given shape, size and concentration of a mixture of nanostructured particles and silver ions in organic and aqueous dispersions with both antimicrobial and anti-toxic effects.

The composition contains silver, a stabilizer and a solvent, while silver contains a binary mixture of a colloidal solution of nanostructured silver particles with a particle size of 2-100 nm and silver ions (cations), and contains a non-polar solvent from the group of saturated hydrocarbons, distilled water, or deionized water, as a stabilizer contains a solution of a hydro-containing polymer, or an acrylic polymer or copolymer in a non-polar solvent, or an aqueous solution of an acrylic polymer, or copolymer, or a binary mixture of a hydroxyl-containing polymer and an aqueous solution of an acrylic polymer, or a copolymer, and additionally contains a solution of a water-repellent based on organosilicon compounds in a non-polar solvent or in an aqueous dispersion.

The proposed method of obtaining a binary colloidal mixture of nanostructured silver particles and silver ions in the stabilizer consists in the recovery of silver ions in the reverse micelle system present in the solution during the synthesis of silver nanostructured particles in an aqueous dispersion, as a result of which the antimicrobial and anti-toxic activity is increased compared with the known compositions.

"Monodisperse colloidal aqueous solution of silver ions with antimicrobial and anti-toxic effects (options) and methods for their preparation" [patent for invention No. 2609176 dated 06/09/2015. Publ. January 30, 2017, Byul. No. 1.] refers to the field of nanotechnology and nanochemistry, and more specifically to metal citrates, which have both antimicrobial and anti-toxic effects, and is currently used in the perfumery, food industry, medicine, agriculture, biology and other various fields of science industry and ecology.

The technical result of the present invention is to obtain stable monodisperse colloidal aqueous solutions of silver cations when they are obtained from nanostructured silver particles in the presence of citric acid with a given concentration in aqueous solutions that have both antimicrobial and antitoxic effects.

A monodisperse colloidal aqueous solution of silver cations, including active metals in the form of citrates, a reducing agent of silver ions in an aqueous dispersion, water distilled or deionized, as the active metal contains a monodisperse colloidal solution of silver cations in an aqueous dispersion, the reducing agent is selected from the group of organic acids, in which additionally introduced stabilizer cations of silver and water-repellent. As a stabilizer of silver cations, it contains an aqueous solution of a hydro-containing polymer, or an aqueous solution of an acrylic polymer or copolymer, or a binary mixture of a hydroxyl-containing polymer and an aqueous solution of an acrylic polymer, or copolymer, and additionally contains an aqueous solution of a water-repellent based on organosilicon compounds based on organosilicon compounds in an aqueous dispersion.

The proposed method of producing monodisperse colloidal aqueous sereba cations comprises the step of obtaining a polydispersed colloidal mixture nanostructured silver particles in the aqueous dispersion and the step of direct interaction between the obtained nanostructured silver particle solution in water with a reducing silver cations while forming a monodispersed colloidal aqueous silver cation solution having antimicrobial and antitoxic action from toxic impurities and microbiological contaminants.

The analysis of the well-known technical solutions patented in the Russian Federation, adopted as prototypes, showed that despite their high efficiency of antimicrobial and anti-toxic properties, the absence of toxic impurities in the formulations, high stability and safety of their application in various fields of science and technology, these So far, technical solutions have not been applied to create the claimed “Composition with the supramolecular structure of a colloidal mixture of complex nanostructures silver or hydrosol particles of silver cations in an aqueous or aqueous-organic solution with antimicrobial and anti-toxic effects and methods for its preparation "for a number of inherent limitations, primarily due to:

- high stability of synthesized micelles of nanostructured silver particles in water dispersion, associated primarily with the developed biochemical technology of their production in water dispersion at given ratios of the constituent components and temperature-time regime [RF Patent №2601757 of 09.06.2015] and as a result, there is no practical possibility of obtaining pure silver cations in aqueous solutions without the presence of residues of nanostructured silver particles in them, which significantly limits their safe areas of applications in biology and medicine;

- the complexity and duration of the cycles of the technological process, synthesized controlled recovery of nanostructured silver particles into silver cations in aqueous or aqueous-organic dispersions "[RF Patent №2609176 dated 06.06.2015], which significantly increases the labor costs for their production and reduces economic feasibility their further application in practice.

Thus, the current lack of technologies for obtaining highly purified compositions of a binary colloidal mixture of nanostructured particles of silver and silver ions in a stabilizer, which have a high selective ability to translate them into a monodisperse colloidal aqueous solution of silver cations, which have antimicrobial and anti-toxic effects, which can later be used for development and creation of a new “Composition with the supramolecular structure of a colloidal mixture of complex nanostructured compounds silver particles or hydrosol of silver cations in an aqueous or aqueous-organic solution with antimicrobial and antitoxic effects and methods for its preparation ”without manifesting the above mentioned disadvantages inherent in all analyzed known technical solutions is an actual scientific and practical task, and therefore the need to develop and the creation of modern bionanotechnology of their production.

To eliminate the above disadvantages in the process of developing a new generation of colloidal formulations based on monodisperse colloidal solutions of silver cations, we paid serious attention, first of all, to the targeted physical and chemical design of nanostructured particles and strictly controlled recovery of the silver cations from them with given concentration, physical parameters and biological properties.

In the process of research and the results we found that improving the quality of obtaining nanostructured silver particles with further transformation to obtain from them aqueous solutions of the Hydrosol silver cations could be achieved by improving the previously developed technologies for producing nanostructured silver particles and silver cations [RF patents №2601757 from 09.06.2015, the Russian Federation №2609176 from 09.06.2015,] for clarifying the ingredients of the used initial components and technological percentages included in the composition ssov their preparation with the specified parameters of size distribution and their concentration in aqueous solutions in the presence of a reducing agent and its ecological purity.

First of all, we achieved such results by improving the quality of production of nanostructured silver particles and physico-chemical control of the silver cation recovery process to obtain highly purified aqueous solutions of silver cations hydrosol with given controlled parameters of silver cations being restored, their concentration and stability in aqueous solutions.

The task to be solved by the claimed invention is to obtain a new class of compositions with supramolecular structure synthesized based on complex compounds of nanostructured particles of silver and / or hydrosol of silver cations and association of small particles, such as molecules, oligomers, clusters of complex chemical and biological substances. distributed in an aqueous or aqueous-organic medium and held together by intermolecular forces to form stabilized synthesized with structures with antimicrobial and anti-toxic effects.

The technical result of the present invention is that a new composition has been obtained with a supramolecular structure synthesized on the basis of complex compounds of nanostructured silver particles and / or a silver cation hydrosol combined with the association of branched and rarefied network molecular structures of complex low molecular weight and high molecular chemical and biological substances distributed in an aqueous or aqueous-organic medium and held together by intermolecular forces to form iem stabilized synthesized structures possessing antibacterial and antitoxic effect. At the same time, the spectrum of antimicrobial and antitoxic action and the effectiveness of the whole range of compositions are maintained for all variants of the total content of the complexing components of the composition.

The above problem is solved by the fact that in a composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or aqueous-organic solution, which has an antimicrobial and anti-toxic effect, including active metals in the form of a binary mixture of a colloidal solution of silver nanostructural particles with a particle size of 2-100 nm and silver ions in an aqueous dispersion, or a colloidal solution of silver cations, a silver cation reducing agent selected from Dips of organic food acids, including citric acid, or ascorbic acid, a stabilizer of a binary mixture of a colloidal solution of nanostructured particles of silver and silver ions or silver cations in an aqueous dispersion in the form of an aqueous solution of a hydroxyl-containing polymer, including either polyvinyl alcohol, or chitin, or chitosan, or cellulose or amylose, or 2-hydroxyethyl methacrylate, or a binary mixture of a hydroxyl-containing polymer and an aqueous solution of an acrylic polymer or copolymer in the form of a secondary acrylic dispersion, distilled or deionized water. At the same time, as a colloidal solution of silver cations, the composition contains a silver cation hydrosol in an aqueous or aqueous-organic solution, which additionally introduced complex emulsifier compounds in the form of surfactant sodium dioctyl sulfosuccinate, which performs the additional function of a stabilizer, dispersing agent involved in the reduction of silver in the form of polyvinylpyrrolidone heterocyclic compound, which performs the additional function of a stabilizer, a thickening agent in the form of glycerin, a complexion constituents of biologically-active substances in the form of oyster mushroom mycelium extract "OVODORIN" colloidal carrier and a mixture of complex compounds propylene glycol.

Wherein:

First, the composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or aqueous-organic solution, which has an antimicrobial and anti-toxic effect, contains silver as a silver colloidal mixture of an aqueous dispersion of nanostructured silver particles and silver cations. emulsifier in the form of surfactant sodium dioctyl sulfosuccinate and as a stabilizer contains sodium carboxymethylcellulose sodium salt and water in the following ratio and mass components,%:

- nanostructured silver particles  - 0.00399-0.0118; - silver cations - 0.0008-0.0024; - emulsifier - 0.015-0.035; - stabilizer - 0.25-0.5; - water, the rest - up to 100.

Secondly, the composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured particles of silver or hydrosol of silver cations in an aqueous or aqueous-organic solution, which has an antimicrobial and anti-toxic effect, as silver contains the hydrosol of silver cations in an aqueous dispersion in a mixture of silver cation reducing agent in in the form of citric acid, or ascorbic acid and an emulsifier in the form of a surfactant, sodium dioctyl sulfosuccinate, which functions as a stabilizer, and water when followed present component ratio% by weight:

- silver cations - 0,00046-0,00092; - silver cation reducing agent - 0.15-0.2; - emulsifier, it is a stabilizer - 0.015-0.035; - water, the rest - up to 100.

Thirdly, the composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or aqueous-organic solution, which has an antimicrobial and anti-toxic effect, which as silver contains a hydrosol of silver cations in an aqueous dispersion in a mixture of silver cation reducing agent in the form of citric acid, or ascorbic acid and emulsifier in the form of surfactant sodium dioctyl sulfosuccinate, dispersing agent in the form of a heterocyclic compound p olivinylpyrrolidone, which is involved in the reduction of silver and simultaneously performs the secondary function of a stabilizer, and water in the following ratio of components,%, mass:

- silver cations - 0,00046-0,00092; - silver cation reducing agent - 0.15-0.2; - emulsifier - 0.015-0.035; - dispersing agent, it is also a stabilizer - 0.5-1.0; - water, the rest - up to 100.

Fourth, the composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured particles of silver or hydrosol of silver cations in an aqueous or aqueous-organic solution, which has an antimicrobial and anti-toxic effect, as silver contains the hydrosol of silver cations in an aqueous dispersion in a mixture of silver cation reducing agent in in the form of citric acid, or ascorbic acid and emulsifier in the form of surfactant sodium dioctyl sulfosuccinate, as a stabilizer contains 0.1-0.3% (mass) of an aqueous solution and a hydroxyl-containing polymer polyvinyl alcohol and water in the following ratio of components,%, mass:

- silver cations - 0,00046-0,00092; - silver cation reducing agent - 0.15-0.2; - emulsifier - 0.015-0.035; - stabilizer - 0.002-0.004; - water, the rest - up to 100.

Fifth, a composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or aqueous-organic solution, which has an antimicrobial and anti-toxic effect, as silver contains a hydrosol of silver cations in an aqueous dispersion in a mixture of silver cation reducing agent in in the form of citric acid, or ascorbic acid and emulsifier in the form of surfactant sodium dioctyl sulfosuccinate, as a stabilizer contains a binary mixture of 0.1-0.3% (mass) hydr ksilsoderzhaschego polyvinyl alcohol polymer and 0.1-0.3% (wt), or an aqueous solution of acrylic polymers, water-based varnish, or a copolymer at a ratio of 1: 1 wt%, and water in the following ratio% by weight:

- silver cations - 0,00046-0,00092; - silver cation reducing agent - 0.15-0.2; - emulsifier - 0.015-0.035; - stabilizer - 0.002-0.004; - water, the rest - up to 100.

Sixth, the composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or aqueous-organic solution, which has an antimicrobial and anti-toxic effect, as silver contains a hydrosol of silver cations in an aqueous dispersion in a mixture of silver cation reducing agent in in the form of citric acid, or ascorbic acid and emulsifier in the form of surfactant sodium dioctylsulfosuccinate, as a stabilizer contains 0.1-0.3% (mass) of an aqueous solution of g droksilsoderzhaschego polymer polyvinyl alcohol as a colloidal carrier comprises a mixture of complex compounds propilengikol as complexing biologically active agents comprises oyster "OVODORIN" mycelium extract, as a thickening agent comprises glycerin and water in the following ratio% by weight:

- silver cations - 0.00092-0.00139; - silver cation reducing agent - 0.2-0.25; - emulsifier - 0.015-0.035; - stabilizer - 0.002-0.004; - carrier of colloidal mixture complex compounds - 10.0-55.0; - complexing  biologically active substances - 0.5-1.5; - thickener - 0.25-30.0; - water, the rest - up to 100.

Seventh, in the method of obtaining a composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or aqueous-organic solution, including the stage of obtaining a polydisperse colloidal mixture of nanostructured silver particles in an aqueous dispersion at a selected concentration of an aqueous solution of ammonium salt silver and the concentration of surface-active substances, varying from 5.0 to 8.0, depending on the degree of hydration, and direct interaction obtained of a nanostructured silver particles in water with a reducing agent of silver cations with the formation of an aqueous colloidal solution of silver cations, to which is added a stabilizer in the form of an aqueous solution of a hydroxyl-containing polymer, or a binary mixture of a hydroxyl-containing polymer and an aqueous solution of an acrylic polymer or copolymer in the form of a secondary acrylic dispersion, then the mixture is stirred for 20-30 minutes until the formation of the reaction dispersion medium and cooled to a temperature of 20-25 ° C; cations of colloidal silver solution is produced in three stages.

At the same time, in the first stage, an inverse micellar solution of nanostructured silver particles with a concentration of nanostructured silver particles of 0.0399-0.0508% (mass) based on surfactant sodium bis-2-ethylhexyl sulfosuccinate in a non-polar solvent is obtained by reducing silver ions in the system reverse micelles preparation comprising obratnomitsellyarnoy reducing agent dispersion from the group of flavonoids having a molar concentration of from 6,6⋅10 ^-4 to 3,3⋅10 ^-3 M in a nonpolar solvent from the group of saturated hydrocarbons: n-hexane whether n-heptane, or n-octane, or n-decane, or cyclohexane, or isooctane, sodium bis-2-ethylhexyl sulfosuccinate surfactant surfactant with a molar concentration of 0.09 to 0.1 M and the introduction of silver ions into it in the form of an aqueous solution of the salt of the metal Ag, with a molar concentration of 3.7⋅10 ^-2 to 5.0 М10 ^-2 M, while the preparation of the reverse-molecular-size dispersion of the reducing agent of nanostructured silver particles is carried out with stirring of the reducing agent and the surfactant in a non-polar solvent at a temperature of 60-80 ° C with reverse refrigeration nobody for 0.5-1.0 hours, followed by cooling the prepared mixture to a temperature of 20-25 ° C and filtration, the introduction of silver ions into it is carried out in the form of an aqueous solution of an ammonium silver salt.

At the second stage, the initial concentrate of the aqueous dispersion of nanostructured silver particles with a concentration of nanostructured silver particles of 0.0399-0.0508% (mass) is obtained, for which the reverse molecular solution of nanostructured silver particles based on the bis-2- surfactant obtained in the first stage ethylhexyl sulfosuccinate sodium in a non-polar solvent is injected with distilled or dionized water, stirred, settled and the primary aqueous mixture of nanostructured silver particles is separated from the non-polar solvent; Primary aqueous mixture of nanostructured silver particles with the presence of an excess of non-polar solvent and surfactant is heated to a temperature of 50-60 ° C for 1-2 hours until complete removal of an excess of non-polar solvent, then cooled to a temperature of 20-25 ° C and maintained at this temperature for 24 hours until complete separation of the surfactant from the aqueous primary mixture.

In the third stage, the hydrosol of silver cations in an aqueous solution is obtained in 1 cycle, for which purpose the working solution is prepared of an aqueous dispersion of nanostructured silver particles in concentrations of 0.5-1.0% by weight, or 1.0-1.5% by weight, of the original the concentration of a colloidal aqueous solution of nanostructured silver particles is 0.0399-0.0508% (mass) and is heated to a temperature of 80-90 ° C, then in a prepared working solution of an aqueous dispersion is injected, respectively, or 0.15-0.2% by mass, or 0.2-0.25% of the mass, the reducing agent of silver cations from the group of organic food acids and sew the resulting binary mixture with a mixer at a mixing speed of 80-100 rpm for, respectively, 7.0-10.0 hours, or 10.0-15.0 hours, until opalescence appears in it, after which the reaction mixture is cooled to 20-25 ° C, maintained at this temperature for 1-2 hours until the disappearance of opalescence and the formation of a hydrosol of silver cations in an aqueous dispersion with a concentration of silver cations, respectively, or 0.00046-0,00092% of mass, or 0.00092-0, 00139% of the mass. The duration of the mixing cycle of the reaction mixture in a given temperature-time interval of 80-90 ° C depends on the ratio of the concentrations of working solutions of the aqueous dispersion of nanostructured silver particles and the reducing agent of silver cations in the water dispersion and for a given ratio of concentrations of mass% of nanostructured silver particles and the reducing agent of silver cations , respectively, is (%) mass:

0.5-1.0: 0.15-0.2: 7.0-10.0 hour;

1.0-1.5: 0.2-0.25: 10.0-15.0 hours.

After that, the prepared aqueous solution of silver cations hydrosol with a given silver concentration is transferred to a separate stirred reactor with a mixing speed of 80-100 rpm, the aqueous solution of silver cations hydrosol is reheated to a temperature of 45-50 ° C and additionally sequentially injected and / or dispersing agent involved in the reduction of silver in the form of a heterocyclic compound polyvinylpyrrolidone, or / and thickener in the form of glycerin, or / and complexing biologically active substances in the form of ec Oyster mushroom OVODORIN tract, or / and the carrier of the colloidal mixture of propylene glycol complex compounds, and / or distilled or deionized water, then the mixture is stirred for 0.5-1.0 hours until a homogeneous reaction dispersion medium is formed, cooled to a temperature of 20- 25 ° C and get one or another form of composition with the supramolecular structure of a colloidal mixture of complex silver compounds in the stabilizer, which has antimicrobial and antitoxic effects.

Eighth, in the method of obtaining a composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or aqueous-organic solution, to obtain a composition with the supramolecular structure of a colloidal mixture of complex compounds of silver nanostructured particles in a stabilizer, the resulting aqueous solution binary mixtures of nanostructured particles of silver and silver ions in the emulsifier in the form of surfactant sodium dioctyl sulfosuccinate with a silver concentration of 0.5-1, 0% of the mass obtained from the initial silver concentration of 0.0508% (mass) is reheated to 45-50 ° C, and during the process of mixing the mixture, the sodium salt and water are successively added to the stabilizer carboxymethylcellulose, then the mixture is stirred with a speed of mixing -100 rpm to form a composition with the supramolecular structure of a colloidal mixture of an aqueous solution of nanostructured silver particles in a stabilizer and cooled to a temperature of 20-25 ° C in the following mass ratio,%:

- nanostructured silver particles - 0.00399-0.0118; - silver ions - 0.0008-0.0024; - emulsifier - 0.015-0.035; - stabilizer - 0.25-0.5; - water, the rest - up to 100.

Ninth, in the method of obtaining a composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured particles of silver or a hydrosol of silver cations in an aqueous or aqueous-organic solution, to obtain a composition with the supramolecular structure of a colloidal mixture of complex compounds of a hydrosol of silver cations in a stabilizer, the resulting aqueous solution hydrosol of silver cations in a mixture of a reducing agent of silver cations in the form of citric acid, or ascorbic acid and an emulsifier in the form of a surfactant sodium d ioctyl sulfosuccinate, which also performs the secondary function of a stabilizer, is reheated to a temperature of 45-50 ° C and water is sequentially added to the mixture during mixing, then the mixture is mixed with a mixing speed of 80-100 rpm to form a composition with the supramolecular structure of a colloidal mixture of complex Hydrosol compounds of silver cations in the stabilizer and cooled to a temperature of 20-25 ° C in the following ratio of mass components,%:

- silver cations - 0,00046-0,00092; - silver cation reducing agent - 0.15-0.2; - emulsifier, it is a stabilizer - 0.015-0.035; - water, the rest - up to 100.0.

Tenth, in the method of obtaining a composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured particles of silver or a hydrosol of silver cations in an aqueous or aqueous-organic solution, to obtain a composition with the supramolecular structure of a colloidal mixture of complex compounds of a hydrosol of silver cations in a stabilizer, the resulting aqueous solution hydrosol of silver cations in a mixture of a reducing agent of silver cations in the form of citric acid, or ascorbic acid and an emulsifier in the form of a surfactant sodium d Ioctyl sulfosuccinate is reheated to 45-50 ° C and during the mixing process the dispersing agent in the form of a heterocyclic compound polyvinylpyrrolidone, which is also involved in the reduction of silver and also the secondary function of the stabilizer, is simultaneously added to it, and then the mixture is mixed with a mixing rate of 80- 100 rpm to form a composition with the supramolecular structure of a colloidal mixture of complex hydrosol compounds of silver cations in a stabilizer and cooled to t temperature 20-25 ° C in the following ratio of mass components,%:

- silver cations - 0,00046-0,00092; - silver cation reducing agent - 0.15-0.2; - emulsifier - 0.015-0.035; - dispersing agent, it is also a stabilizer - 0.5-1.0; - water, the rest - up to 100.

Eleventh, in the method of obtaining a composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or in an aqueous-organic solution, to obtain a composition with the supramolecular structure of a colloidal mixture of complex compounds of a hydrosol of silver cations in a stabilizer, the resulting aqueous solution hydrosol of silver cations in a mixture of a reducing agent of silver cations in the form of citric acid, or ascorbic acid and an emulsifier in the form of a surfactant nat Dioctyl sulfosuccinate is reheated to 50-60 ° C and during the mixing process the stabilizer is sequentially added to it in the form of 0.1-0.3% (mass) of an aqueous solution of a hydroxyl-containing polymer polyvinyl alcohol and water, then the mixture is mixed with a stirring speed of 80 -100 rpm to a composition with the supramolecular structure of a colloidal mixture of complex hydrosol compounds of silver cations in a stabilizer and cooled to a temperature of 20-25 ° C in the following mass ratio,%:

- silver cations - 0,00046-0,00092; - silver cation reducing agent - 0.15-0.2; - emulsifier - 0.015-0.035; - stabilizer - 0.002-0.004; - water, the rest - up to 100.0.

Twelfth, in the method of producing a composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or aqueous-organic solution, to obtain a composition with the supramolecular structure of a colloidal mixture of complex compounds of a hydrosol of silver cations in a stabilizer, the resulting aqueous solution silver cations in a mixture of reducing cations of silver in the form of citric acid, or ascorbic acid and an emulsifier in the form of a surfactant sodium dioct sulfosuccinate is reheated to 50-60 ° C and in the process of mixing the mixture, sequentially add to it a stabilizer in the form of a binary mixture of 0.1-0.3% (mass) of a hydroxyl-containing polymer polyvinyl alcohol and 0.1-0.3% (mass) aqueous solution of acrylic polymers, water-based varnish, or copolymers at a ratio of 1: 1 mass,% and water, then the mixture is mixed with a mixing speed of 80-100 rpm to a composition with the supramolecular structure of a colloidal mixture of silver hydrosol complex compounds in a stabilizer and cool to a temperature of 20-25 ° C in the following ratio of mass components,%:

- silver cations - 0,00046-0,00092; - silver cation reducing agent - 0.15-0.2; - emulsifier - 0.015-0.035; - stabilizer - 0.002-0.004; - water, the rest - up to 100.0.

Thirteenth, in the method of producing a composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or aqueous-organic solution, to obtain a composition with the supramolecular structure of a colloidal mixture of complex compounds of a hydrosol of silver cations in a stabilizer, the resulting aqueous solution hydrosol of silver cations in a mixture of a reducing agent of silver cations in the form of citric acid, or ascorbic acid and an emulsifier in the form of a surfactant nat Dioctyl sulfosuccinate is reheated to 45-50 ° C and, while mixing the mixture, the stabilizer is sequentially added to it in the form of 0.1-0.3% (mass) of an aqueous solution of a hydroxyl-containing polymer polyvinyl alcohol, the carrier of a colloidal mixture of complex compounds of propylene glycol, complexing biological compounds active substances oyster mushroom extract oyster mushroom OVODORIN, thickener glycerin and water, then the mixture is mixed with a mixing speed of 80-100 rpm until a composition is formed with a supramolecular collage structure an ideal mixture of complex compounds of the hydrosol cations of silver in the stabilizer and cooled to a temperature of 20-25 ° C in the following ratio of mass components,%:

- silver cations - 0.00092-0.00139; - silver cation reducing agent - 0.2-0.25; - emulsifier - 0.015-0.035; - stabilizer - 0.002-0.004;

- carrier of colloidal mixture

complex compounds - 10.0-55.0;

- complexing biologically active

thing - 0.5-1.5; - thickener - 0.25-30.0; - water, the rest - up to 100.

The minimum and maximum values of the composition of the composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or hydrosol of silver cations in an aqueous or in aqueous-organic solution of chemical ingredients, expressed in% of their mass, are based primarily on their practical importance of the manifested properties of the declared variants of a binary mixture of nanostructured particles of silver and silver ions, and / or a hydrogel of silver cations, combining with the association of branched and diluted network molecular structures of complexing chemical and biological substances distributed in an aqueous or aqueous-organic medium.

The options presented in the description of the application are selected on the basis of the physical and chemical state of chemical and biological ingredients established on the basis of theoretical and experimental studies that are part of the composition with the supramolecular structure of a colloidal mixture of complex compounds of silver nanostructures or hydrosols of silver cations in water or in water -organic solution, resulting in a change in density, viscosity and state of aggregation of the colloidal p itself The solution is based on their specified properties.

According to the results of the research, we found that the complexing chemical and biological substances introduced into the composition, when combined with nano-sized silver particles or silver cations, do not “mask”, i.e. do not reduce the biological effectiveness of the proposed variants of the composition relative to their antimicrobial and antitoxic action.

These substances include:

- stabilizer in the form of complex compounds of the emulsifier surfactant sodium dioctyl sulfosuccinate;

- a reducing agent of silver cations selected from the group of organic food acids, including either citric acid or ascorbic acid;

- stabilizer in the form of an aqueous solution of a hydroxyl-containing polymer, comprising either polyvinyl alcohol, or chitin, or chitosan, or cellulose, or amylose, or 2-hydroxyethyl methacrylate, or a binary mixture of a hydroxyl-containing polymer and an aqueous solution of an acrylic polymer or copolymer in the form of a secondary acrylic dispersion;

- dispersing agent in the form of a heterocyclic compound of polyvinylpyrrolidone, which performs the additional function of a stabilizer;

- thickener in the form of glycerin;

- complexing biologically active substances in the form of an extract of mycelium of oyster mushroom "OVODORIN";

- carrier of a colloidal mixture of complex compounds propylene glycol.

Thus, in the implementation of the proposed method of production of the claimed variants, at the end of the technological process in a shortened time interval, a composition with the supramolecular structure of chemical and biological substances in an aqueous or aqueous-organic solution is obtained.

Introduced into the composition of the proposed variants of the composition of a binary colloidal mixture of nanostructured particles of silver and silver cations, or hydrzole of silver cations in combination with the association of branched and rarefied network molecular structures of complexing chemical and biological substances distributed in an aqueous or aqueous-organic medium with a given controlled concentration and entering the composition of the ingredients of these substances does not include toxic nanoparticles of metal oxides and hydroxide nanoparticles s metals, as well as impurities chloride, nitrate and sulfate ions.

The absence of the obtained colloidal solution of the proposed variants of the composition with the supramolecular structure of chemical and biological substances of the above impurities provides a high controlled stability and environmental safety of colloidal solutions of the formed compositions for a long time, thereby ensuring their sufficient antimicrobial activity and antitoxic action.

A new method has been developed for obtaining a composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or aqueous-organic solution, which includes a new process algorithm for producing an aqueous solution of a silver cation hydrosol.

In the present method, in contrast to the known method (see RF patent №2609176 from 09.06.2015) to obtain a composition with the supramolecular structure of a colloidal mixture of complex silver compounds, including a stage of obtaining a polydisperse colloidal mixture of nanostructured silver particles in an aqueous dispersion and direct interaction of the resulting solution nanostructured silver particles in water with a reducing agent of silver cations, obtaining at the 3 stage an aqueous solution of the hydrosol of silver cations with the formation of its working solutions, is produced in 1 qi l for, respectively, 7.0-10.0 hours, or 10.0-15.0 hours, until the appearance of opalescence in it, depending on the ratios of the selected concentrations of the reducing agent of silver cations from the group of organic food acids (or 0.5- 1.0% by weight, or 1.0-1.5% by weight) of the selected initial concentration of a colloidal aqueous solution of nanostructured silver particles 0.0399-0.0508% (mass).

After obtaining the Hydrosol of silver cations with a given concentration of them to obtain a composition with the supramolecular structure of a colloidal mixture of complex silver compounds, this solution additionally introduces or / and a dispersing agent involved in silver reduction in the form of a heterocyclic compound polyvinylpyrrolidone, or / and a thickener in the form of glycerin, or / and complexing biologically active substances in the form of an extract of mycelium of oyster mushroom "OVODORIN", or / and wear v colloidal mixture of complex compounds propilengikol, or / and water are distilled or deionized to obtain the or a variant of the inventive composition with supramolecular structure colloidal mixture of complex compounds nanostructured silver particles or hydrosol of silver cations in an aqueous or aqueous organic solution having antibacterial and antitoxic effect.

As a result, the inherent in the known compositions of compositions with the supramolecular structure of a colloidal mixture of complex silver compounds with chemical and biological substances and technologies for their preparation were eliminated existing drawbacks that reduce the effectiveness or limit their targeted use as agents that simultaneously have antimicrobial action and a mechanism for the transformation of chemical substances with toxic properties in the transformation of safe organic (inorganic) substances.

When analyzing the known technical solutions, the authors did not find a similar composition with the supramolecular structure of a colloidal mixture of complex silver compounds with chemical and biological substances and methods for their preparation, which have antimicrobial and antitoxic effects, with a set of essential features specified in the proposed technical solution, which proves the conformity of the proposed technical solutions to the criteria of the invention of "novelty."

In the studied technical solutions, which are included in the level of technology, the authors did not reveal the effect of the transformations, prescribed by the proposed technical solution, characterized by the essential features that are distinctive from the prototype, on the achievement of this technical result, which proves the compliance of the proposed technical solution with the inventive step criterion of the invention.

In practice, the method of obtaining a composition (its variants) with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or aqueous-organic solution is carried out in 3 stages:

Stage 1 The method of obtaining the composition of a binary colloidal mixture of nanostructured particles of silver and silver ions includes the following technological operations:

1. Obtaining a concentrate of nanostructured metal particles by reducing metal ions in the reverse-emulsion dispersion of a reducing agent with a concentration of 0.0399-0.0508% (mass), which corresponds to 3.7-4.7 * 10 ^-3 g ion / l, including following operations:

- the dissolution of the surfactant in a non-polar solvent with a temperature of 20-25 ° C;

- preparation of the inversely micellar dispersion of flavonoid, which includes mixing the solution of surfactant, non-polar solvent and flavonoid at a temperature of from 60 ° C to 80 ° C under reflux for 0.5-1 hour;

- preparation of an aqueous solution of the ammonium salt of the metal at a temperature of 20-25 ° C;

- mixing the cooled inversely micellar dispersion of flavonoid with an aqueous solution of the ammonium metal salt at a temperature of 20-25 ° C until a homogeneous micellar solution is obtained.

2. Obtaining concentrate aqueous dispersion of nanostructured metal particles from their reverse micellar solution with their concentration of 0.0399-0.0508% (mass), including the following operations:

- add distilled water in a micellar solution in a ratio by volume of 1: 1.0-1: 1.5;

- the resulting mixture is vigorously stirred at a temperature of 20-25 ° C for 5-10 minutes;

- the mixed mixture is settled for 1-3 hours before the visualized clear boundary of the separation of the organic phase of the primary aqueous mixture of nanostructured silver particles with the presence of an excess of non-polar solvent and surfactant in it at a temperature of 20-25 ° C;

- the primary aqueous mixture is separated from the organic phase;

- the separated primary aqueous mixture is heated to a temperature of 50-60 ° C for 1-2 hours until complete removal of the excess non-polar solvent from the mixture;

- the heated primary mixture is cooled to a temperature of 20-25 ° C and maintained at this temperature for 24 hours until complete separation of the surfactant surplus from the primary aqueous mixture containing nanostructured silver particles;

- the aqueous mixture is separated from the surfactant surplus until the finished product is obtained — an aqueous dispersion of nanostructured silver particles with a concentration of 0.0399-0.0508% (mass).

3. Obtaining an aqueous solution of nanostructured silver particles with a concentration of 0.5%, 1.0%, 1.5% (mass) of nanostructured silver particles from their initial concentration of 0.0399-0.0508% (mass).

Stage 2 The method of obtaining silver cations Hydrosol with the formation of its working solutions (variants) includes the following technological operations:

In accordance with the claimed invention, the necessary option to obtain the Hydrosol of silver cations in an aqueous dispersion with the desired concentration of silver (respectively, or 0.00046%, or 0.00092%, or 0.00139% of the mass) is carried out in 1 cycle, for which:

- prepare a working solution of an aqueous dispersion of nanostructured silver particles with a concentration of either 0.5%, or 1.0%, or 1.5% by weight, of the initial concentration of a colloidal aqueous solution of nanostructured silver particles of 0.0508% (mass) and heat it to temperature 80-90 ° C;

- in the prepared working solution of nanostructured silver particles with a silver concentration of either 0.5% or 1.0%, respectively, 0.15% or 0.2% by weight of a reducing agent of silver cations from the group of organic food acids are injected, and in the prepared working a solution of nanostructured silver particles with a silver concentration of either 1.0% or 1.5% of the mass is injected, respectively, or 0.2% or 0.25% of the mass of the reducing agent of silver cations from the group of organic food acids and the resulting binary mixture is stirred with a stirrer at a stirring speed 80-100 rpm for Respectively, or 7.0-10.0 hours, or 10.0-15.0 hours, until the appearance of opalescence in it, after which the reaction mixture is cooled to 20-25 ° C, kept at this temperature for 1-2 hours to the disappearance of opalescence and the formation of a hydrogel of silver cations in an aqueous dispersion with a concentration of silver cations, respectively, or 0.00046%, or 0.00092%, 0.00139% of the mass;

- prepared versions of the Hydrosol of silver cations with a concentration of silver cations, respectively, or 0.00046%, or 0.00092%, or 0.00139% of masses are used to produce the claimed composition variants with the supramolecular structure of a colloidal mixture of complex compounds of the hydrosol complex of silver cations in water or water. -organic solution.

Stage 3 Methods of obtaining the claimed variants of the composition with the supramolecular structure of a colloidal mixture of complex compounds of the hydrosol of silver cations in an aqueous or aqueous-organic solution include the following process steps:

To obtain the claimed variants of the composition with the supramolecular structure of a colloidal mixture of complex hydrosol compounds of silver cations in an aqueous or aqueous-organic solution prepared with a given (respectively, either 0.00046%, or 0.00092%), or 0.00139% by weight, cation concentration silver, an aqueous solution of silver cation hydrogel is transferred to a separate stirred reactor with a mixing speed of 80-100 rpm, the selected variant of the silver cation solution is reheated to a temperature of 45-50 ° C and additionally However, a polyvinylpyrrolidone, or / and a thickener in the form of glycerin, or / and complexing biologically active substances in the form of an oyster mushroom extract OVODORIN, or / and a carrier of a colloid mixture of complex, is introduced either a dispersing agent that participates in the reduction of silver as a heterocyclic compound. compounds of propylene glycol, or / and distilled or deionized water, then the mixture is stirred for 0.5-1.0 hours until a homogeneous reaction dispersion medium is formed, cooled to a temperature of 20-25 ° С and I get some form supramolecular structure with the composition of the colloidal mixture of complex compounds of nanostructured particles and silver cations, or hydrosol of silver cations in the stabilizer having antibacterial and antitoxic effect. Wherein:

1. In the first stage, to obtain the claimed variants of the composition of a binary colloidal mixture of nanostructured particles of silver and silver ions, the following raw materials were used:

- silver nitrate (AgNO ₃ ) according to GOST 1277;

- reference isooctane according to GOST 12433;

- sodium dioctyl sulfosuccinate (C ₂₀ H ₃₇ Na O ₇ S, CAS 577-11-7) (aerosol OT or AOT);

- quercetin (C ₁₅ H ₁₀ O _72. H ₂ O, pentahydroxyflavone, CAS 6151-25-3);

- water ammonia “OFS” according to GOST 24147;

- distilled water, GOST 6709-72.

With that:

The concentration of the metal salt varied from 3.7⋅10 ^-2 to 5.0⋅10 ^-2 M The ratio of molar concentrations of water and surfactant in the reverse-cellral dispersion of the metal salt (degree of hydration, W = [H2O] / [AOT] selected in the range of 5 to 8.

The molar concentration of the substance from the group of flavonoids in the reverse micellar dispersion of the reducing agent was chosen in the range from 6.6⋅10 ^-4 to 3.3⋅10 ^-3 M.

2. In the second stage, to obtain the proposed variants of an aqueous solution of the hydrosol of silver cations with the formation of its working solutions (variants), the following raw materials were used:

- silver concentrate in water dispersion "Neosilver concentrate-Bio" for the production of biologically active food additives with a silver concentration of 12.0 mg / l., TU 9185-008-17572054-15, EAC. Eurasian Economic Union Declaration of Conformity N RU D- RU. AG47.V.13085 from 01.06.2017;

- citric acid monohydrate food, GOST 908-2004;

- distilled water, GOST 6709-72.

3. At the third stage, to obtain the claimed composition options with the supramolecular structure of a colloidal mixture of silver hydrosol complex compounds in an aqueous or aqueous-organic solution, the following types of raw materials were used:

- concentrate of silver cations in the aqueous dispersion "AKVIVON ^® " with a concentration of colloidal silver of 8.5-12.5 mg / l, TU 20.42.15-038-87552538-16, AC Eurasian Economic Union Declaration of Conformity NRU D-RU. AB05.V.14961 of February 18, 2016;

- concentrate of silver cations in the water-organic dispersion "PROMETEY", TU 20.42.15-040-87552538-17 with a concentration of silver cations C = 7.5-9.0 mg / l, EAC Customs Union Declaration of Conformity TC N RU D -RU AG47.V.13238 from 13.06.2017;

- “Ovodorin oyster mushroom extract“ OVODORIN ^® ”according to TU 9317-032-87552538-15, Declaration of Conformity TC N RU D-RU. AB05.V.01738 dated 10/28/2015;

- Food supplement E1201 "Polyvinylpyrrolidone" Mark "Co. Vidone® K30". Boai NKY Pharmaceuticals Ltd Manufacturer China, Certiflcat of Analysis, Report No: RE05S01-201508002-0;

- Food supplement E466 “Carboxymethylcellulose sodium salt, CAS 9004-32-4, Manufacturer:“ Foodchem international Corporation ”Specification of batch №20140428 from 08.28-14-14;

- Food supplement "Glycerol" (E 422) (European Pharmacology, CAS-No. 56-81-5, EG 200-289-5). Manufacturer Germany, certificate of analysis AZ-14-184, Certificate of State Registration No. BY.70.06.01.009E.00374607.14 of July 07, 2014;

- Food additive "Propylene glycol (Propyleneglycol) USP / EP" (EU Directive 2000/63 / EC with amendments 96/77 / EC (food additives) USP, Eur., JP) PRS-CODEX, manufacturer Germany, specification, Certificate Registration No. RU.77.99.26.009.E.005872.03.11 of 03/16/11;

- Food supplement E460 “Microcrystalline cellulose FLOCEL 102 (MCC 102), CAS 9004-34-6, Declaration of Conformity TC N RU D-DE.АЯ46.B.75650 from 02.26.2015;

- polyvinyl alcohol grade 1599, CAS number: 9002-89-5, country of origin: Germany, dated March 18, 2011;

- water-based lacquer for furniture and walls in accordance with TU 2316-028-48797870-2015, SGR No. RU.77.01.34.008.E.000891.04.16 of 04/21/20 16;

- acrylic varnish VD-AK-1113;

- water dispersion of acrylic copolymer Novopol-002A, Novopol-002B;

- purified water FS.2.020.15.

With that:

1. Concentrate silver cations in aqueous dispersion "AKVIVON ^®» with the concentration of colloidal silver 8.5-12.5 mg / l 20.42.15-038-87552538-16 TU, AU Eurasian economic union declaration of compliance D N RU-RU . АБ05.В.14961 from 02.18.2016 in its composition will contain the following initial components:

- colloidal silver according to TU 20.42.15-038-87552538-16, EAC Customs Union Declaration of Conformity TC N RU D-АБ05. B.14961 dated 02/18/2016;

- surfactant sodium dioctyl sulfosuccinate (C ₂₀ H ₃₇ NaO ₇ S) CAS 577-11-7, non-citric acid monohydrate food GOST 908-2004;

- distilled water, GOST 6709-72.

2. Concentrate of silver cations in the water-organic dispersion "PROMETEY", TU 20.42.15-040-87552538-17 with a concentration of silver cations C = 7.5-9.0 mg / l, EAC Customs Union Declaration of Conformity TC N RU D-RU AG47.V.13238 of June 13, 2017 will contain the following basic components:

- Concentrate of silver cations in the aqueous dispersion "AKVIVON ^® " with a concentration of colloidal silver of 8.5-12.5 mg / l, TU 20.42.15-038-87552538-16, AC Eurasian Economic Union Declaration of Conformity N RU D-RU. AB05.V.14961 of February 18, 2016;

- Food additive E1520 “Propylene glycol (Propyleneglycol) USP / EP” according to the manufacturer’s specification. State Registration Certificate No. RU 77.99.26.009.E.005872.03.11 of 03/18/2011

3. The developed technology of industrial multistage cultivation of the mycelium of the fungus Pleurotus ostreatus (Fr.) Kumm (strain 1137, VKPM F 819) [RF patent for invention №2487930 dated 19.06.2012. Bul No. 20 of 07/20/2013]] allows you to adjust the composition of biologically active substances in the produced oyster mushroom mycelium extract [Gerasimenya V.P. Innovative biotechnology of industrial cultivation of fungi Pleurotus ostreatus (Fr.) Kumm, used in pharmaceutical practice for the creation of medical products: monograph / V.P. Gerasimenya, S.V. Zakharov, V.M. Brusnikin, M.A. Klykov, L.P. Semasheva; by ed. V.P. Gerasimeni, V.Yu. Polyakova. - M .: Institute of Chemical Physics. N.N. Semenova RAS, LLC Inbiopharm, 2013. - 212 p.].

As a result of the research [VP Gerasimenya. Extracts of basidiomycetes and their multifunctional medico-biological activity: monograph / V.P. Gerasimenya, K.Z. Gumargaliyeva, S.V. Zakharov, T.I. Milevich, A.V. Trezvova; by ed. V.P. Gerasimeni, V.Yu. Polyakova. - M .: Institute of Chemical Physics. N.N. Semenova, Ltd. "Inbiofarm", 2014. -. 128], it was found that the obtained "extract of oyster mushroom mycelium" OVODORIN ^® »TU 9317-032-87552538-15, declaration of compliance TC D N RU-RU. АБ05.В.01738 from 10.28.2015 in its composition will contain the following initial components in the natural ratio of the masses:

- carbohydrates (glucose, galactose, mannose, arabinose, xylose, galactose, glucosamine);

- amino acids (asparagine, series, threonine, glutamine, proline, glycine, alanine, valine, leucine, lysine, histidine, arginine, cysteine, methionine, tyrosine, phenylalanine);

- fatty acids (C10-C22);

- organic acids (butyric, lactic, acetic, malic, oxalic);

- vitamins (B1, B2, B6, PP, D, E, C),

- metals and trace elements (sodium, potassium, calcium, magnesium, phosphorus, sulfur, iron, zinc, manganese, selenium, copper, aluminum, boron, barium, silicon, lithium, etc.);

- water up to 30%.

Below are examples of the implementation of the invention for the proposed method for producing a composition (its variants) with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or aqueous-organic solution

Example 1. Preparation of 1000.0 ml of a binary colloidal mixture concentrate of nanostructured particles of silver and ions in an aqueous dispersion with a silver concentration of 0.0399% (mass).

In 960 ml of isooctane dissolve 40 g of sodium dioctyl sulfosuccinate at 20-25 ° C and add 300-400 mg of quercetin. The resulting mixture is heated in a flask under reflux to 60-70 ° C and stirred for 05-1 hours, after which the heated mixture is cooled to 20-25 ° C and filtered.

9.0 ml of an aqueous solution of the ammoniacal silver salt are added to the previously obtained quartzcetin micellar solution. The mixture is stirred at 20-25 ° C for 1-3 minutes to obtain a homogeneous dark micellar solution of nanostructured silver particles with a concentration of 0.0399% (mass).

To 1000 ml of micellar solution of nanostructured particles of silver, obtained according to example 1, add 1000-1500 ml of distilled water and vigorously mix at a temperature of 20-25 ° C for 5-10 minutes. Next, the mixed mixture is settled for 1-3 hours until the visualized clear boundary between the organic phase of the primary aqueous mixture of nanostructured silver particles and the presence of an excess of isooctane and dioctyl sodium at a temperature of 20-25 ° C, then the primary aqueous mixture of silver nanostructured particles is separated from isooctane.

The separated primary aqueous mixture of nanostructured silver particles is heated to a temperature of 50 ° -60 ° C for 1 hour until complete removal of the excess isooctane from the mixture. After that, the heated aqueous mixture of nanostructured silver particles is cooled to a temperature of 20-25 ° C and maintained at this temperature for 24 hours to obtain the finished product — an aqueous dispersion of nanostructured silver particles with a concentration of 0.0399% (mass).

After receiving the finished product with a concentration of 0.0399% (mass), or 0.5%, or 1.0%, or 1.5% (mass), an aqueous solution of a binary colloidal mixture of nanostructured silver particles and ions from the initial concentration of nanostructured silver particles 0.0399% (mass) prepared by diluting it with distilled water at a temperature of 20-25 ° C.

Example 2. Obtaining 1000.0 ml of a concentrate of a binary colloidal mixture of nanostructured particles of silver and ions in an aqueous dispersion of silver with a silver concentration of 0.0508% (mass).

Dissolution of dioctyl sodium sulfosuccinate in a non-polar solvent and the preparation of the reverse-molecular dispersion of flavonoid at a temperature of 20-25 ° C is performed as in Example 1.

Next, the resulting mixture is heated in a flask under reflux to a temperature of 60-80 ° C and stirred for 05-1 hour, after which the heated mixture is cooled to 20-25 ° C and filtered.

12.0 ml of an aqueous solution of ammoniacal silver salt are added to the previously obtained quartzcetin micellar solution. The mixture is stirred at 20-25 ° C for 1-3 minutes to obtain a homogeneous dark micellar solution of nanostructured silver particles with a concentration of 0.0508% (mass).

The introduction of distilled water into a micellar solution of nanostructured silver particles, their mixing, settling and separation of the primary aqueous mixture of nanostructured silver particles from isooctane is carried out as in Example 1.

Next, the separated primary aqueous mixture of nanostructured silver particles is heated to a temperature of 50-60 ° C at 60 ° C with stirring for 1 hour until complete removal of the excess isooctane from the mixture.

Cooling the heated primary mixture with its exposure for 24 hours and separating excess sodium dioctyl sulfosuccinate from the aqueous mixture to obtain the finished product — an aqueous dispersion of nanostructured metal particles is produced as in Example 1 to obtain the finished product — an aqueous dispersion of silver nanostructured particles with a concentration of 0.0508% ( masses).

After receiving the finished product with a concentration of 0.0508% (mass), prepare an aqueous solution of a binary colloidal mixture of nanostructured particles of silver and ions with a silver concentration of either 0.5% or 1.0% or 1.5% (mass) of the original the concentration of nanostructured silver particles of 0.0508% (mass) is prepared by diluting it with distilled water at a temperature of 20-25 ° C.

Example 3. Obtaining 1000.0 ml of a composition with the supramolecular structure of a colloidal mixture of complex compounds of the hydrosol of silver cations in an aqueous solution with a silver concentration of 0.00092%, (5.64 mg / l) of mass:

In accordance with the claimed formula p. 10 of the invention, the selected option to obtain a composition with the supramolecular structure of a colloidal mixture of complex compounds of the hydrosol of silver cations in an aqueous solution with a selected silver concentration of 0.00092%, is as follows:

- prepare 1000 ml of an aqueous solution of nanostructured silver particles with a concentration of 1.0%, mass, from the initial concentration of 0.0508% (mass) of a binary colloidal mixture of nanostructured particles of silver and silver ions as in Example 2 and heated to a temperature of 80-90 ° C ;

- in the prepared working solution of nanostructured silver particles with a silver concentration of 1.0%, mass, from the initial concentration of 0.0508% of the mass of the binary colloidal mixture of nanostructured particles of silver and silver ions, 0.2% citric monohydrate acid is added to the food and the resulting mixture is stirred with stirring speeds of 80-100 rpm for 10.0-15.0 hours until the appearance of opalescence in it, after which the reaction mixture is cooled to 20-25 ° C, kept at this temperature for 1-2 hours until it disappears opalescence and formation of hydrosol to thiones silver in aqueous solution with a concentration of silver cations 0.00092 wt% (5.64 mg / l).

Example 4. Obtaining 1000.0 ml of a composition with the supramolecular structure of a colloidal mixture of complex compounds of the hydrosol of silver cations in an aqueous solution, with a silver concentration of 0.00046%, (2.96 mg / l) mass:

In accordance with the claimed formula p. 11 of the invention, the selected option to obtain a composition with the supramolecular structure of a colloidal mixture of complex compounds of the hydrosol of silver cations in an aqueous solution with a silver concentration of 0.00046%, is as follows:

- prepare 1000 ml of an aqueous solution of nanostructured silver particles with a concentration of 0.5%, mass, from the initial concentration of 0.0508% (mass) of a binary colloidal mixture of nanostructured particles of silver and silver ions as in Example 2 and heated to 80-90 ° C ;

- in the prepared working solution of a binary colloidal mixture of nanostructured particles of silver and silver ions with a silver concentration of 0.5%, (mass) of the initial concentration of 0.0508% (mass) of a binary colloid mixture of nanostructured particles of silver and silver ions enter 0.15% acid citric monohydrate food and mix the resulting mixture with a stirrer at a mixing speed of 80-100 rpm for 7.0-10.0 hours, until opalescence appears in it, after which the reaction mixture is cooled to 20-25 ° C, kept at this temperature within 1-2 hours before disappearing Nia hydrosol formation and opalescence of silver cations in the aqueous dispersion with the concentration of silver cations 0.00046% (2.96 mg / l) wt.

Next, the resulting aqueous solution of the silver cation hydrosol is reheated to 45-50 ° C, and during the mixing process, 0.5% of the dispersing agent in the form of a heterocyclic compound of polyvinylpyrrolidone, which participates in the reduction of silver and also has the secondary function of a stabilizer, is subsequently added to it, then the mixture is mixed at a mixing rate of 80-100 rpm until a composition is formed with the supramolecular structure of a colloidal mixture of complex compounds of the hydrosol complex of silver cations in st abilizer with a concentration of silver cations of 0.00046% in it, (2.96 mg / l) of masses and cooled to a temperature of 20-25 ° C.

Example 5. Obtaining 1000.0 ml of a composition with the supramolecular structure of a colloidal mixture of complex compounds of the hydrosol of silver cations in an aqueous solution with a silver concentration of 0.00046%, (2.96 mg / l) mass:

In accordance with the claimed formula p. 12 of the invention, the selected option to obtain a composition with the supramolecular structure of a colloidal mixture of complex compounds of the hydrosol of silver cations in an aqueous solution with a silver concentration of 0.00046%, is as follows:

- prepare 1000 ml of an aqueous solution of nanostructured silver particles with a concentration of 0.5%, mass, from the initial concentration of 0.0508% (mass) of a binary colloidal mixture of nanostructured silver particles as in Example 2 and heated to 80-90 ° C;

- in the prepared working solution of nanostructured silver particles with a silver concentration of 0.5%, mass, from the initial concentration of 0.0508% (mass) of a binary colloidal mixture of nanostructured silver particles, 0.15% citric monohydrate acid is added to the food and the resulting mixture is stirred with a stirrer at a speed mixing 80-100 rpm for 7.0-10.0 hours until the appearance of opalescence in it, after which the reaction mixture is cooled to 20-25 ° C, kept at this temperature for 1-2 hours until the disappearance of opalescence and the formation of silver cations in water ispersii with silver cations concentration 0.00046% (2.96 mg / l) wt. Next, the resulting monodisperse colloidal solution of silver cations is reheated to 50-60 ° C and in the process of mixing the mixture, the stabilizer is sequentially added to it in the form of a 0.2% (mass) aqueous solution of a hydroxyl-containing polymer polyvinyl alcohol and water. The mixture is then mixed at a mixing rate of 80-100 rpm until a composition is formed with the supramolecular structure of a colloidal mixture of complex compounds of the hydrosol of silver cations in a stabilizer with a concentration of silver cations of 0.00046% (2.96 mg / l) mass and cooled to a temperature of 20-25 ° C.

Example 6. Obtaining 1000.0 ml of a composition with the supramolecular structure of a colloidal mixture of complex compounds of silver cations in an aqueous solution with the formation of its working solution with a silver concentration of 0.00092% mass, (5.64 mg / l) mass:

In accordance with the claimed formula p. 13 of the invention, the selected option to obtain a composition with the supramolecular structure of a colloidal mixture of complex compounds of the hydrosol of silver cations in an aqueous solution with a silver concentration of 0.00092%, is as follows:

- prepare 1000 ml of an aqueous solution of nanostructured silver particles with a concentration of 1.0%, mass, from the initial concentration of 0.0508% (mass) of a binary colloidal mixture of nanostructured silver particles as in Example 2 and heated to a temperature of 80-90 ° C;

0.2% citric monohydrate acid is added to the prepared working solution of nanostructured silver particles with a silver concentration of 1.0% by weight, starting from a concentration of 0.0508%) (mass) of a binary colloidal mixture of nanostructured silver particles, and the resulting mixture is stirred with a stirrer mixing 80-100 rpm for 10.0-15.0 hours, until the appearance of opalescence in it, after which the reaction mixture is cooled to 20-25 ° C, kept at this temperature for 1-2 hours until the disappearance of opalescence and formation of hydrosol silver cations in aqueous solution with a silver cation concentration of 0.00092%), (5.64 mg / l) mass.

Next, the resulting aqueous solution of the silver cations Hydrosol is reheated to 50-60 ° C and in the process of mixing the mixture, the stabilizer is sequentially added to it in the form of a binary mixture of 0.1% (mass) hydroxyl-containing polymer polyvinyl alcohol and 0.1% (mass) of an aqueous solution acrylic polymer, in the form of a water-based lacquer for furniture and walls, or acrylic VD-AK-1113 lacquer, or a copolymer in the form of a water dispersion of an acrylic copolymer Novopol-002A or Novopol-002B at a ratio of 1: 1 wt.%, and water.

The mixture is then mixed at a mixing rate of 80-100 rpm until a composition is formed with the supramolecular structure of a colloidal mixture of complex compounds of the hydrosol of silver cations in a stabilizer with a concentration of silver cations of 0.00092%, (5.64 mg / l) and cooled to a temperature of 20-25 ° C.

Example 7. Obtaining 1000.0 ml of a composition with the supramolecular structure of a colloidal mixture of complex hydrosol compounds of silver cations in an aqueous-organic solution with a silver concentration of 0.00139% by weight, (8.52 mg / l) of masses.

In accordance with the claimed formula p. 14 of the invention, the selected option to obtain a composition with the supramolecular structure of a colloidal mixture of complex compounds of the hydrosol of silver cations in an aqueous-organic solution with a silver concentration of 0.00139%, is as follows:

- prepare 1000 ml of an aqueous solution of nanostructured silver particles with a concentration of 1.5%, mass, from the initial concentration of 0.0508% (mass) of a binary colloidal mixture of nanostructured silver particles as in Example 2 and heated to 80-90 ° C;

- in the prepared working solution of nanostructured silver particles with a silver concentration of 1.5%, mass, from the initial concentration of 0.0508% (mass) of a binary colloidal mixture of nanostructured particles of silver and silver ions, 0.2% citric monohydrate food grade is added and the resulting mixture is mixed with a stirrer at a stirring speed of 80-100 rpm for 10.0-15.0 hours, until opalescence appears in it, after which the reaction mixture is cooled to 20-25 ° C, kept at this temperature for 1-2 hours to the disappearance of opalescence and the formation of hydrosol to silver ions in aqueous solution with a concentration of silver cations of 0.00139% by weight, (8.52 mg / l) of masses.

Next, the resulting aqueous solution of the hydrosol of silver cations is reheated to 45-50 ° C. While mixing the mixture, 0.02% of the stabilizer mass is added in the form of 0.1-0.3% (mass) of an aqueous solution of a hydroxyl-containing polymer polyvinyl alcohol, 10 , 0% of the mass of the carrier of the colloidal mixture of complex compounds of propylene glycol, 15.0% of the mass of the complexing biologically active substances of the oyster mushroom extract OVODORIN, 10.0% of the masses of a thickener glycerin and the rest, up to 100.0% of water.

The mixture is then mixed at a stirring speed of 80–100 rpm until a composition is formed with the supramolecular structure of a colloidal mixture of complex compounds of the hydrosol of silver cations in a stabilizer with a concentration of silver cations of 0.00139% by mass, and (8.52 mg / l) mass and cooled to a temperature of 20-25 ° C.

Control over the formation of nanostructured silver particles and silver ions in the binary mixture, as well as the hydrosol of silver cations and the assessment of their stability, was carried out spectrophotometrically based on changes in the main characteristics of the optical absorption spectra (for nanostructured silver particles) the position of the absorption band maximum and the optical density at the absorption band maximum. in the region of 400–425 nm, and for stabilized silver cations in an aqueous solution of a hydrosol of silver cations - the position of the band maximum is absorbed I and the optical density at the maximum absorption bands in the region 465-490 nm.).

The presence of nanostructured silver particles in a binary colloidal mixture, as well as the presence of silver cations in an aqueous solution of a hydrosol, was determined by two methods:

- methods of spectral analysis, respectively in the region of 400-425 nm and 465-490 nm, using a two-beam spectrophotometer "HELIOS ALPHA" (UK) [TU 9185-025-87552538-12 "Concentrate of colloidal silver in aqueous dispersion". - M .: LLC Inbiopharm, 2012. - 16 p .; Pyatnitsky II, Sukhan V.V. Analytical chemistry of silver / I.I. Pyatnitsky, V.V. Suhan. - M .: Science, 1975. - 264 p.];

- by the method of atomic emission spectrometry with inductively coupled plasma (AES-ICP), using an ICAP-6500 Duo (England) plasma device, ITEVA software [O. Dementeva, M. Filippenko, T. Andreeva and others. Grew. Nanotechnology 2012. T 7 No. 9-10. - p. 54-63; Sedukh E.M., Dementeva O.V., Rudoy V.M., et aii // Book of abstrakts ESAS 2014, 16-21 March. Prague, Czech Republic, - P. 231].

For effective use of the developed variants of the claimed composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or aqueous-organic solution, the physical parameters of an aqueous solution of a silver cation hydrosol were studied during the studies.

As a result of the study of the total silver content in the composition of the aqueous solution of the silver cations Hydrosol, it was found that in the prepared variants with a silver cation concentration of 0.5%, 1.0%, 1.5% (mass), the total amount of silver is respectively 0.00046% , or 0.00092%, or 0.00139% of the mass (respectively, or 2.96 mg / l, or 5.64 mg / l, or 8.52 mg / l) of an aqueous solution, which does not contradict the results of the qualitative control of the presence silver cations in similar cases by the method of controlling the assessment of the reliability of the content in the composition of the aqueous p Silver Crescent Hydrosol solution using a HELIOS ALPHA (UK) dual-beam spectrophotometer with a microprocessor control system confirms the reliability of their production using the inventive options for producing a composition (or variants) with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or cations of silver in aqueous or in aqueous-organic solution.

Thus, as a result of the research conducted on the development and creation of a composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or in an aqueous-organic solution with antimicrobial and anti-toxic effects, we obtained documentary evidence describing the control process on the nanoscale level (spectral characteristics, etc.) of nanotechnological redistribution (nanostructures formed in an aqueous solution of a binary mixture x silver particles and silver ions in the process of reduction of silver cations in water dispersion) in the process of their production.

To confirm the biological effectiveness of the claimed composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or silver cations in an aqueous or aqueous-organic solution and the method of its preparation, we conducted laboratory tests of samples of aqueous solutions of the developed composition options (see examples 1-7) at the level of in vitro with the determination of their antimicrobial and anti-toxic activity.

The study of the antimicrobial activity of the developed variants of the composition at the in vitro level was carried out on the prepared samples:

- for 1.5% concentration of a binary mixture of nanostructured particles of silver and silver ions in an aqueous dispersion, obtained from the initial concentration of nanostructured particles of silver 0.0508% of the mass (variant No. 1);

- for three concentrations of an aqueous solution of a silver cation Hydrosol with a silver concentration of 0.5% 1.0% 1.5%, obtained respectively from 0.5%, 1.0% and 1.5% by weight of an aqueous solution of nanostructured silver particles (versions №1, 2, 3)

by diffusion in agar (wells) in accordance with [GF XII (Diffusion method in agar OFS 42-0068-07) and Workshop on microbiology, edited by A.I. Netrusova. - M .: Publishing center "Academy", 2005. - 606 p.].

The method is based on the ability of antibiotic substances to diffuse into agar media and form zones in which test organisms do not grow. The size of the zone of no growth indicates the degree of activity of this antibiotic substance in relation to the test organism.

Antimicrobial activity of the studied samples of drugs tested on test cultures:

- Gram-positive - Micrococcus flavus and spore Bacillus mycoides;

- Gram-negative - Escherichia coli 113/3;

- microscopic fungi - Aspergillus niger and Penicillium chrysogenum (collection of the Department of Microbiology, Lomonosov Moscow State University).

Bacteria were grown on MPA, micromycetes were grown on Saburo medium of the following composition (g / l): 40.0; peptone - 10.0; agar - 20.0; chloramphenicol - 2.5%.

As prototypes of the composition were tested manufactured versions of the samples with the content in their composition:

but). Binary mixture of nanostructured particles of silver and silver cations in an aqueous dispersion with a silver concentration of 1.5% by weight of their initial concentration of 0.0508% (mass), in accordance with Example No. 2 described in the description of the invention:

- option number 1 (sample number 1), C = 1.5%;

b). An aqueous solution of the Hydrosol of silver cations with a silver concentration of 0.00046%, 0.00092%, 0.00139% of the mass obtained from a binary mixture of nanostructured particles of silver and silver ions in an aqueous dispersion with a silver concentration of 0.5%, 1.0%, 1.5% by weight, in accordance with the examples given in the description of the invention No. 3-6:

- option number 2 (sample number 2), C = 0.00092% (mass);

- option number 3 (sample number 3), C = 0.00046% (mass);

- option number 4 (sample number 4), C = 0.00139% (mass).

The manufacture of the holes in the agar produced as follows. Test cultures that were previously grown on shoals in agar media were washed from the shoals with saline to obtain a bacterial suspension with a cell count of 1 × 10 ⁹ (OD = 0.9 light filter No. 6, cuvette 10) or according to an industry standard turbidity sample (turbidity TO) , developed by the GOSNI standardization and control of medical biological products to them. L.A. Tarasevich, Moscow.

The suspension was added to MPA (for bacterial cultures) or Saburo medium (for micromycetes) and poured into Petri dishes with a layer of 20 ml.

A sterile metal drill (pretreated with 70% alcohol and flamed in the flame of the burner) in a frozen agar medium with a test culture made holes with a diameter of 7 mm.

Methods and results of tests to determine the antimicrobial activity of the developed versions of the samples of the claimed composition

In the wells of agar with the test culture, prepared by the method described above, 900 μl of each of the studied samples was added. To study the spectrum of the antimicrobial action, 6 variants of the prepared samples in replicates of at least 3 were used. Sterile distilled water (volume 900 μl), also inoculated into the well, was used as a control. After daily incubation at temperatures optimal for the growth of cultures (37 ° C for bacteria Micrococcus flavus and Bacillus mycoides, 42 ° C for E. coli, and for fungi Aspergillus niger and Penicillium chrysogenum - 28 ° C) and for the next 6 days measurements were made of the sizes of diameters of the growth inhibition of the test cultures relative to the wells with the test samples.

The antimicrobial effect of the samples was compared with the activity of antibiotic preparations. Were used indicator paper discs, impregnated with antibiotics. For gram-positive bacteria (Micrococcus flavus and Bacillus mycoides), disks with ristomycin (25 µg), an antibiotic belonging to the group of polycyclic glycopeptides, are used to suppress the synthesis of nucleic acids. Ristomycin is widely used to treat infections caused by gram-positive bacteria. With respect to gram-negative (Escherichia coli 113/3) discs with levomycetin (30 µg) were used, which belong to the family of aromatic antibiotics. Levomitsetin (chloramphenicol) inhibits protein synthesis, has a wide range of bactericidal action, but does not inhibit the growth and development of micromycetes. This antibiotic is widely used in medical practice. For micromycetes (Aspergillus niger and Penicillium chrysogenum), disks with nystatin (15 μg) belonging to the family of polyene antibiotics that have fungicidal activity were used.

According to the test results, the growth inhibition values of Penicillium chrysogenum, Bacillus mycoides, Escherichia coli 113/3, Aspergillus niger and Penicillium chrysogenum on an agar medium of the studied mixtures of the nanostructured particles of silver and silver cations and samples of the aqueous solution of hydrosol of silver cations were found within 1-6 days compared with the control of antibiotics Ristomycin, Levomycetin and Nystatin. The test results are presented in tables 1-5.

As a result of testing the developed variants of samples of composition No. 1-4 according to the above-mentioned method of determining the action spectrum of the studied variants of the samples, it was found that practically all tested 4 variants of the samples significantly exceed all the control values of the studied antibiotics in their antibacterial and fungicidal activity by the values of the antibacterial and fungicidal activity throughout the 6 days of observation.

It should be noted that the advantage of the activity of the tested samples over the activity of the known antibiotics is objectively confirmed by the inhibition values of the test cultures on the agar medium from the 1st to the 6th day of observation, inclusive.

So, it was found that in the first 3 days, all concentrations (from 0.5% to 1.5%) of the test samples tested inhibit the growth of all tested test organisms at the level of inhibition of known antibiotics used. Starting from 4 days before the end of the test (6 days), all 6 tested prototypes on the inhibition activity of test cultures do not reduce the level of the effectiveness of the concentrations of preparations established in the first 3 days, while the antibiotics used are Nystatin for inhibiting Penicillium chrysogenum, chloramphenicol for inhibiting E.coli113 / 3 and Ristomycin for inhibiting Micrococcus flavus completely lose their antimicrobial activity, as a result of which the secondary growth of test cultures occurs on the control Petri dishes with these variants.

Thus, the experimentally established facts of the antibacterial and fungicidal activity of all the tested samples, prepared variants of the preparations in accordance with the examples given in the description and studied under laboratory conditions on test cultures in an agar medium certainly confirm the technical result of the claimed composition with the supramolecular structure of the colloidal mixture of complex compounds nanostructured particles of silver or hydrosol of silver cations in an aqueous or aqueous organic solution ore, which has antimicrobial and anti-toxic effects.

The method and results of tests to determine the antitoxic effect of the developed versions of the samples of the claimed composition. The effectiveness of the antitoxic action (catalytic properties) of the claimed composition with the supramolecular structure of the colloidal mixture of complex compounds of nanostructured silver particles or hydrosol of silver cations in aqueous or in aqueous-organic solution was studied using the examples of manufactured 4 variants of air filter cartridges used in the cleaning simulation process and air volatile carbohydrates (test air pollutants: acetone and carbon dioxide vapors) dissolved in the air of an insulated sealed box according to the developed method on a special laboratory test-measuring complex created [patent for the invention of the Russian Federation No. 2400286 of March 13, 2009, publ. 09/27/2010 Bull. №27. Filtering material for the purification of liquid and gaseous substances and a method for producing it] when filtering air pollutants with a porous carrier (cartridge) of an air filter with an active catalyst applied to its surface in the form of manufactured versions of the claimed composition with a given silver concentration.

The fabrication of prototypes of filtering material (cartridges) with stabilized or nanostructured silver particles or silver cations applied on the surface of the filtering material was carried out as follows.

In the experimental development of variants of the filter material used:

- as a porous base - synthetic non-woven hydrophobic roll material [GOST R 51251-99, CENEN 779-1993; CENEN 1822-1998] brand FM-3X, h = 15 mm, cleaning class G3, fiber type polyester;

- as an active catalyst:

- 1.5% aqueous solution of a binary colloidal mixture of nanostructured particles of silver and silver ions from the initial concentration of nanostructured particles of silver 0.0508% (mass) obtained in example 2;

- 1.0 aqueous solution of the hydrosol of silver cations obtained in example 3, from the initial concentration of nanostructured silver particles of 1.0% (mass);

- 1.5% aqueous solution of hydrosol of silver cations, obtained according to example 5, from the initial concentration of nanostructured silver particles of 1.5% (mass);

- 1.5% aqueous solution of silver cations Hydrosol from the initial concentration of nanostructured silver particles 1.5% (mass), stabilized with 0.2% aqueous solutions of PVA grade 11/2, 16/1, highest grade or first grade, GOST 10779-78 in the amount of 2.0% by weight of the binary mixture obtained in example 6.

Modification of the filtering material with nanostructured silver particles or a hydrosol of silver cations was performed as follows.

Variants of examples of obtaining a filtering material: Option 1. In accordance with Example 2 described in the description of the application, 1 liter of an aqueous solution of silver nanoparticles with a concentration of 1.5% of an aqueous solution of a binary colloidal mixture of nanostructured silver particles and silver ions from an initial solution of silver concentration 0 is obtained, 0508% (mass). To apply a binary mixture of nanostructured silver particles and silver ions (catalyst) on a porous carrier of filter material, place a plate made of a synthetic non-woven fabric of the brand FM-3X, cleaning class G3, h = 15 mm, 330 × 330 mm in size at a temperature of 20-25 ° С in the prepared aqueous solution and maintained at this temperature for 1 hour. Next, the plate is dried at 40-50 ° C for 3-4 hours. Option 1 of a cartridge coated with a catalyst on its surface is ready for use.

Option 2. In accordance with Example No. 3 described in the description of the application, 1 liter of the composition is prepared with a content of 0.00092% of an aqueous solution of a silver cation hydrosol from 1.0% (mass) of an aqueous solution of a binary mixture of nanostructured silver particles and silver ions. In the prepared solution is placed a plate of non-woven material brand FM-3X, cleaning class G3, h = 15 mm, size 330 × 330 mm and impregnated porous base material for 5-10 minutes at a temperature of plus 20-25 ° C, then dried at temperature plus 60-70 ° C for 3-4 hours to establish their constant weight. Variants 2 of the cartridge, with a catalyst deposited on their surface, are ready for use.

Option 3. In accordance with Example 5 described in the description of the application, 1 l of a composition is prepared with a content of 0.00046% (mass) of an aqueous solution of a silver cation Hydrosol from 0.5% (mass) of an aqueous solution of a binary mixture of nanostructured silver particles and silver ions stabilized 0.2% aqueous solutions of PVA brand 11/2, 16/1, highest grade or first grade, GOST 10779-78 in the amount of 2.0% by weight of the mixture of the stabilizer. In the prepared solution is placed a plate of non-woven material brand FM-3X, cleaning class G3, h = 15 mm, size 330 × 330 mm and impregnated porous base material for 5-10 minutes at a temperature of plus 20-25 ° C, then dried at temperature plus 60-70 ° C for 3-4 hours to establish their constant weight. Option 3 of the cartridge coated with a catalyst on its surface is ready for use.

Option 4. In accordance with Example 6 described in the description of the application, 1 l of a composition is prepared with a content of 0.00139% (mass) of an aqueous solution of a hydrosol of silver cations from 1.5% (mass) of an aqueous solution of a binary mixture of nanostructured particles of silver and silver ions, stabilized with a binary mixture of 0.2% aqueous solutions of PVA grade 11/2, 16/1, highest grade or first grade, GOST 10779-78 and acrylic VD-AK-1113 varnish in the amount of 2.0% by weight of the binary mixture. Next, the technological operations are carried out analogously to example 2 or 3.

Filtration efficiency or filtering ability of the made filtering material options for air purification from test air pollutants was performed using air purification from a mixture of extra acetone and carbon dioxide on an example of a special laboratory-measuring complex.

The complex consists of the following parts:

- hermetic box of 200 l, made of stainless steel;

- a computer that performs registration and display of sensor signals in real time.

An electric heater for evaporation of a liquid test substance is placed in the box.

In the side wall of the box there are unions for inputting gaseous test pollutants into the box and connecting an external gas sensor.

Stationary gas analyzers of the IGS-98 series, produced by the FSUE SPE DELTA, are used to measure the concentration of volatile carbohydrates in the process of testing. Gas sensors required for testing are connected to the data recording system of the control and measuring complex.

To record the concentration of carbon dioxide, an external optical infrared gas sensor IGS-016-00 was used, which has a built-in microprocessor for recirculation of air from the box. The range of measurement of the relative concentration of carbon dioxide is 0-1%. Sensor measurement accuracy ± 0.1%.

To record the concentration of acetone vapors, a gas thermocatalytic hydrocarbon sensor was used.

Pre-thermocatalytic hydrocarbon sensor was calibrated in the test box on the pairs of residual acetone (mV / mg). Sensor measurement accuracy ± 10%.

All gas analyzers are certified in the Russian Federation and passed the state calibration.

Outside the box, there is a power supply and gas sensor signal processing and an electronic signal processing and computer communication unit.

As an experimental sample of an air purifier, a modified commercially available housing with a fan from the Aero Barrier photocatalytic air cleaner, model AP270FC, was used.

In the modified Aero Barrier air cleaner case, successively made versions of the filtering material (see options 1-4) were installed as an air cartridge undergoing testing. An air cleaner with the cartridge installed was placed in the box.

The air cleaner turned on, the mode of operation of its fan was set. AIR AIR EXCHANGE (0 ~ 150 m ³ / h), after which the box was closed. Included all the rest of the equipment.

After some time (~ 10 min.) A binary mixture of test pollutants: carbon dioxide and OFS acetone was injected into the box through the air inlet.

In the course of the experiment, the destruction of pollutants occurs under normal atmospheric conditions while blowing the gas mixture through a nanocatalytic porous membrane (cartridge) with an air filter fan.

During ~ 1.5 ... 2.5 hours, the sensor readings are recorded and automatically recorded into the computer’s memory from the beginning of the filter on the drop in the concentration of the test pollutant in the box over time. Registration and recording of sensor readings is performed within a specified time with a measurement accuracy of 1 s. In this case, a decrease in the concentration level of the test pollutant in the process of deep destruction of the pollutant into the simplest components of the molecule is recorded: (C, H ₂ , O ₂ ).

The rate of destruction of volatile hydrocarbons is represented by a change in the relative concentration over time:% / hour, and is converted into absolute values, mg / hour.

Thus, when testing the manufactured filtering material options for air purification, the efficiency of each variant of the manufactured cartridge for the destruction of test contaminants over time was obtained.

The results of the evaluation of the effectiveness of air purification by an air cleaner from test pollutants, in particular a mixture of carbon dioxide and extra-acetone vapors, by test cartridges made using catalysts based on the claimed composition are shown in Table 6.

As a result of the laboratory tests of the made variants of the filtering material for air purification, the following was established.

During operation of the air cleaner with all applicable options 1-4 of filter material (cartridge), for a given time of air filtration in a sealed box, there is a significant decrease in air concentrations of test pollutants of carbon dioxide and TOC acetone vapors from the concentration originally created in the box.

During the operation of the experimental samples of the air cleaner there is a significant decrease in the concentration of hydrocarbons in acetone from the concentration initially created in the chamber at a rate of destruction of 11.75-21.8 mg / hour, and there is also a significant destruction of carbon dioxide at speeds of 208.7-293.7 mg / hour, with initial concentrations of ~ 0.25% CO ₂ ..

At the same time, the destruction of each pollutant occurs according to a scheme identical to the destruction of a mono pollutant, that is, the efficiency of the filtering material does not depend much on the amount of pollutants in the air without the influence of the process of destruction of one substance on another.

The tests have shown high efficiency of the catalytic properties of the cartridge versions made.

Based on the above own research results and comparing them with the known results of the study of the antimicrobial and antitoxic activity of compositions based on nanostructured silver particles and silver cations in aqueous dispersions, it can be concluded that the claimed composition, possessing both catalytic and disinfectant (bactericidal, fungicidal and virucidal) actions, creates a real barrier to prevent contamination of surfaces of objects and their surrounding air from toxic eskih contaminants and microbiological contaminants, thereby protecting the body against pathogens chemically and biologically hazardous contaminants.

Thus, the invention ensures the achievement of a technical result.

The present invention can be used to obtain a composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous or aqueous-organic solution, which has an antimicrobial and anti-toxic effect used in the perfumery, food industry and medicine for the production of dietary supplements food, cosmetics, medical products, medicines, protective and disinfectants in the form of solutions, gels and whether films with antimicrobial and anti-toxic effects.

Table 1 - The values of inhibition of the growth of Micrococcus flavus on an agar medium with the studied samples of the composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or hydrosol of silver cations in an aqueous solution (variants 1-4, corresponding to examples 2-6), for 1-6 days depending on the concentration in silver samples, equal respectively to 0.00046% of the mass (2.96 μg / ml), 0.00092% of the mass (5.64 μg / ml), 0.00139% of the mass (8.52 μg / ml ) and control of antibiotic Ristomycin

Table 2 - Values of growth inhibition of Bacillus mycoides on agar medium with test samples of the composition with supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or hydrosol of silver cations in an aqueous solution (options 1-4, corresponding to examples 2-6), within 1-6 days depending on the concentration in silver samples, equal respectively to 0.00046% of the mass (2.96 μg / ml), 0.00092% of the mass (5.64 μg / ml), 0.00139% of the mass (8.52 μg / ml ) and control of antibiotic Ristomycin

Table 3 - Values of growth inhibition of Escherichia coli 113/3 on agar medium with test samples of the composition with supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or hydrosol of silver cations in aqueous solution (options 1-4, corresponding to examples 2-6), for 1 -6 days, depending on the concentration in the samples of silver, equal respectively to 0.00046% of the mass (2.96 μg / ml), 0.00092% of the mass (5.64 μg / ml), 0.00139% of the mass (8.52 mkg / ml) and control of an antibiotic Levomitsetin

Table 4 - Values of inhibiting the growth of Aspergillus niger on agar medium with test samples of the composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or hydrosol of silver cations in an aqueous solution (options 1-4, corresponding to examples 2-6), within 1-6 days depending on the concentration in silver samples, equal respectively to 0.00046% of the mass (2.96 μg / ml), 0.00092% of the mass (5.64 μg / ml), 0.00139% of the mass (8.52 μg / ml ) and the control of antibiotic Nystatin

Table 5 - Table 10 - Growth inhibition values of Penicillium chrysogenum on an agar medium with test samples of a composition with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or hydrosol of silver cations in an aqueous solution (variants 1-4, corresponding to examples 2-6), for 1 -6 days, depending on the concentration in the samples of silver, equal respectively to 0.00046% of the mass (2.96 μg / ml), 0.00092% of the mass (5.64 μg / ml), 0.00139% of the mass (8.52 µg / ml) and antibiotic control Nystatin

Table 6 - The results of evaluating the efficiency of air purification with an air cleaner from test contaminants (toxic impurities) by the test cartridges made using a catalyst based on the developed composition options with the supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles or a hydrosol of silver cations in an aqueous solution with antimicrobial and antitoxic action

Claims (6)

1. Composition with supramolecular structure of a colloidal mixture of complex compounds of nanostructured silver particles, which has an antimicrobial and anti-toxic effect, including active metals in the form of a binary mixture of a colloidal solution of nanostructured silver particles with a particle size of 2-100 nm and a colloidal solution of silver cations, reducing silver cations, selected from citric acid and ascorbic acid, the stabilizer of the binary mixture of a colloidal solution of nanostructured particles of silver and silver cations in aqueous distilled in the form of polyvinyl alcohol, distilled or deionized water, characterized in that as a colloidal solution of silver cations, the composition contains a silver cation hydrosol in an aqueous or aqueous-organic solution, which additionally introduced complex compounds of the emulsifier in the form of a surfactant, sodium dioctyl sulfosuccinate, dispersant in the form of a heterocyclic compound of polyvinylpyrrolidone, a thickener in the form of glycerin, complexing biologically active substances in the form of extra ota mycelium oyster mushroom "Ovodorin" and the carrier of a colloidal mixture of complex compounds propylene glycol in the following ratio (wt. %): nanostructured silver particles 0.0399-0.0508 silver cations 0,00092-0,00139 silver cation reducing agent 0.2-0.25 emulsifier 0,015-0,035 dispersing agent 0.002-0.004 carrier of a colloidal mixture of complex compounds 10.0-55.0 complexing biologically active substances 0.5-1.5 thickener 0.25-30.0 water, the rest up to 100 2. A method of obtaining a composition according to claim 1, comprising a stage of obtaining a polydisperse colloidal mixture of nanostructured silver particles in an aqueous dispersion at a selected concentration of an aqueous solution of an ammonia silver salt and a concentration of surfactant varying from 5.0 to 8.0, depending on the degree of hydration, and the direct interaction of the resulting solution of nanostructured silver particles in water with a reducing agent of silver cations with the formation of an aqueous colloidal solution of silver cations, to which is added a stabilizer of an aqueous solution of a hydroxyl polymer, and then the mixture was stirred for 20-30 minutes to form a reaction dispersion medium and cooled to a temperature of 20-25 ^° C, and preparing an aqueous colloidal solution of silver cations is carried out in 3 stages to give the first stage reverse micellar solution of nanostructured silver particles with a concentration of nanostructured silver particles of 0.0399-0.0508% (wt.) based on the surfactant bis-2-ethylhexyl sodium sulfosuccinate in a non-polar solvent, by reducing silver ions in the reverse micelle system, which includes preparation of the inverse micellar dispersion of a reducing agent from the group of flavonoids with a molar concentration of from 6.6 × 10 ⁻⁴ to 3.3 × 10 ⁻³ M in a non-polar solvent from the group of saturated hydrocarbons: n-hexane, or n-heptane, or n-octane, or n-decane, or cyclohexane, or isooctane, surfactant of sodium bis-2-ethylhexyl sulfosuccinate with a molar concentration of from 0.09 to 0.1 M and the introduction of silver ions into it as an aqueous solution of Ag metal salt with a molar concentration ntratsiey from 3,7 × 10 ^-2 to 5,0 × 10 ^-2 M, wherein the reducing agent dispersion preparation obratnomitsellyarnoy nanostructured silver particles are under stirring reductant and a surfactant in a nonpolar solvent at a temperature of 60-80 ^° C to reflux within 0.5-1.0 h followed by cooling the prepared mixture to a temperature of 20-25 ^° C and filtering, the introduction of silver ions into it is carried out in the form of an aqueous solution of an ammoniacal silver salt, in the second stage the initial concentrate of the aqueous dispersion of nanostructures is obtained silver particles with a concentration of nanostructured silver particles of 0.0399-0.0508 (wt. %), for which purpose, obtained in the first stage reverse micelle solution of nanostructured silver particles based on surfactant sodium bis-2-ethylhexyl sulfosuccinate in a non-polar solvent is injected with distilled or dionized water, agitated, defend and separate the primary aqueous mixture of nanostructured silver particles from non-polar the solvent, the separated primary aqueous mixture of nanostructured silver particles with the presence of an excess of non-polar solvent and surfactant in it is heated to a temperature of 50-60 ^° C for 1-2 hours until complete removal of excess non-polar solvent, and then cooled to 20-25 ^° C and kept at this temperature for 24 hours until complete removal of excess surfactant from the aqueous mixture of primary , characterized in that in the third stage, the hydrosol of silver cations in an aqueous or aqueous-organic solution is obtained in 1 cycle, for which purpose a working solution is prepared of an aqueous dispersion of nanostructured silver particles in concentrations of 0.5-1.0 wt. %, or 1.0 to 1.5 wt. % of the initial concentration of the aqueous colloidal solution of nanostructured silver particles is 0.0399-0.0508 (wt.%) and is heated to a temperature of 80-90 ^° C, then the prepared dispersion of the aqueous dispersion is injected respectively or 0.15-0.2 wt. %, or 0.2-0.25 wt. % reducing agent of silver cations from the group of organic food acids and mix the resulting binary mixture with a stirrer at a mixing rate of 80-100 rpm for respectively 7.0-10.0 h, or 10.0-15.0 h until it appears in it opalescence, after which the reaction mixture is cooled to 20-25 ^° C, maintained at this temperature for 1-2 hours until the disappearance of opalescence and the formation of a silver cation hydrosol in an aqueous dispersion with a concentration of silver cations, respectively, or 0.00046-0,00092% wt ., or 0.00092-0.00139% wt., with the duration of The mixing of the reaction mixture in a given temperature-time interval of 80-90 ^° C depends on the ratio of the concentrations of working solutions of the aqueous dispersion of nanostructured silver particles and the reducing agent of silver cations in the aqueous dispersion and for a given ratio of the concentrations of silver cations, respectively (wt. %): 0.5-1.0: 0.15-0.2-7.0-10.0 h; 1.0-1.5: 0.2-0.25-10.0-15.0 h, then the prepared aqueous solution of silver cations hydrosol with a given silver concentration is transferred to a separate stirred reactor with a mixing rate of 80-100 rpm, the aqueous solution of silver cations hydrosol is reheated to a temperature of 45-50 ^° C and the dispersing agent is additionally sequentially introduced polyvinylpyrrolidone as a heterocyclic compound, a thickening agent in the form of glycerol, complexing biologically active substances in the form of an oyster mushroom extract “OVODORIN”, colloidal carrier The mixture of complex compounds of propylene glycol and distilled or deionized water, then the mixture is stirred for 0.5-1.0 hours until a homogeneous reaction dispersion medium is formed, cooled to a temperature of 20-25 ^° C to obtain a composition with the supramolecular structure of a colloidal mixture of silver complex compounds .