RU2634964C1 - Method for reducing risk of development of uterine postnatal inflammatory diseases in cows - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: method includes the injection of biologically active substances with antioxidant effect to cows. Cows 1 are given fumaric, ascorbic, succinic and citric acids in a mixture taken in a certain ratio once a day during 65 days before calving and 10 days after calving.

EFFECT: use of the invention allows to optimize indicators of the level of endogenous intoxication, metabolic processes, which leads to a decrease in the level of obstetric diseases.

13 tbl, 4 ex

Description

The invention relates to veterinary medicine, relates to a method of reducing the risk of developing postpartum inflammatory diseases of the uterus in cows and can be used for therapeutic and prophylactic protection of cows from mass diseases of the reproductive organs and to obtain a physiologically healthy offspring.

Pathologies of the reproductive organs are a local manifestation of polysystemic metabolic disorders and arise against the backdrop of insufficiency of the placental system, disintegration of metabolic processes and a decrease in the protective and adaptive capabilities of the body associated with termination of pregnancy, childbirth, the onset of lactation, structural and functional transformations in the reproductive apparatus and exposure to stress factors biotic and abiotic nature [1, 2]. The metabolic status and response to the pathological factor is determined by the degree of endogenous intoxication, in which there is a pronounced change in the immune status, manifested by immunosuppression [3]. According to the results of studies that do not contradict the literature, the risk criteria for the development of postpartum inflammatory diseases of the uterus in cows are the following changes in immunobiochemical homeostasis 2-2.5 months before delivery: an increase in the level of leukocytes and lymphocytes, the absolute number of lymphocytes, T and B lymphocytes, the content of IgA, lysozyme activity of blood serum, the absorption capacity of neutrophils (PS and PI), a decrease in the content of IgG and IgM, bactericidal activity of blood serum [4]. It has been established that the development of postpartum inflammatory processes of the uterus in cows is accompanied by an increase in the index of endogenous intoxication by more than 20.0% in comparison with clinically healthy animals [5].

Known means and methods for the prevention and treatment of obstetric pathology in cows with an efficiency of 65-90% or more. Almost all treatment and prevention regimens include antibiotics and chemotherapeutic drugs, specific biologically active substances (hormones, prostaglandins, etc.). However, antibiotics, sulfonamides, analgesics, hormones, synthesized vitamins, desensitizing and anti-inflammatory drugs, in addition to the therapeutic effect, also have a negative effect on the body. For example, the high anti-inflammatory activity of glucocorticoids is combined with frequent and often severe side effects. In addition, in some cases severe allergic reactions, hemorrhagic syndromes, hyperglycemia, etc. occur. Many antibacterial drugs are strong immunosuppressants, which worsens the course of the disease and lengthens the recovery period of animals. In addition, the means of etiotropic, symptomatic and nonspecific pathogenetic therapy do not reduce the effect of the toxic factor, which is due to the additional accumulation of microbial toxins as a result of the death of microorganisms.

Known methods of preventing the development of postpartum diseases in cows by normalizing metabolic processes with the use of vitamins, macro- and microelements [6], whose effect only indirectly affects the immune system - the main link in the mechanisms of the body's response to the action of an infectious agent that causes the development of the inflammatory process.

As the closest analogues, the authors consider methods for correcting the immunobiochemical status of cows in the prenatal and postpartum periods using agents that provide antioxidant protection of the body, contribute to the normalization of metabolism and increase resistance by reducing and preventing the accumulation of toxic lipid peroxidation products, for example, a drug containing amber and ascorbic acid [7].

The purpose of the invention is a method of reducing the risk of developing postpartum inflammatory diseases of the uterus in cows using biologically active substances of antioxidant activity - natural metabolites, which provides an effect on the main factors provoking postpartum obstetric pathologies - normalization of hormonal-metabolic, antioxidant and immune homeostasis.

The goal is achieved by reducing the risk of developing uterine inflammation in cows using fumaric, ascorbic, succinic and citric acids in a mixture in a dose and according to a scheme that supports homeostasis and increases adaptive potential by reducing the level of endogenous intoxication.

Succinic and citric acids are important factors in the regulation of the physiological state. They are universal intermediate metabolites in the tricarboxylic acid cycle, which are formed during the interconversion of carbohydrates, proteins and fats in plant and animal cells. The conversion of acids in the body is associated with the production of energy necessary for life support. Succinic and citric acids have antidote properties, cleansing the body of heavy metals, radionuclides, salts of nitric and nitrous acids. In addition, succinic acid has lactoprotective and antiviral effects. Citric acid stimulates the formation in the intestines of chelated forms of calcium, zinc, iron, etc., which are much more easily absorbed by the body. It is reliably known that citric acid is a synergist of succinic acid, enhancing the biological effect of the latter.

Ascorbic acid (vitamin C) plays an important role in redox processes, reactions of carbohydrate and protein metabolism, reduces cholesterol and calcium levels in the blood, reduces the deposition of lipids and calcium in organs, activates the enzymes of the stomach, intestines, has antihemorrhagic effect, prevents the development of infectious beginning in the body, stimulates the exocrine pancreatic function, erythropoiesis and the system of mononuclear phagocytes, provides anti-inflammatory effect of ionized of calcium, activates phagocytosis.

Fumaric acid is a natural metabolite of living organisms, provides emergency ATP synthesis, thereby counteracting stress at the cellular level. It is resistant to oxidation, temperature fluctuations and compatibility with other biologically active substances. The accelerated formation of ATP with the participation of fumaric acid contributes to the conservation of glucose for the synthesis of glycogen in the liver and ascorbic acid in the body. The accumulation of reserve nutrients and ascorbic acid in the liver prolongs the period of its active functioning and ensures the cleansing of the liver of metabolic products.

The method is as follows.

Dry cows 1 time per day for 65 days before and 10 days after calving, fumaric, ascorbic, succinic and citric acids are orally administered in a mixture based on the dose, mg / kg of live weight - fumaric acid - 2.0; ascorbic acid - 3.0; succinic acid - 4.0; citric acid - 1.0.

The essence of the method is illustrated by examples.

Example 1. By the principle of analogues, two groups of dead cows were formed 65-68 days before calving: experimental (n = 21) and control (n = 22). The cows of the experimental group once a day for 65 days before and 10 days after calving were orally administered fumaric, ascorbic, succinic and citric acids based on the dose, mg / kg body weight - fumaric acid - 2.0; ascorbic acid - 3.0; succinic acid - 4.0; citric acid - 1.0. Cows in the control group of drugs were not used. The level of endogenous intoxication of the body and the parameters of immunobiochemical homeostasis were determined by comparing the values of the experimental and control groups, the results of laboratory tests of blood of cows 65-68 (before using the drug), 30-35 days before calving and 10-14 days after calving according to the following indicators: phagocytic neutrophil activity (FAN) (determined in whole blood according to BC Gostev), phagocytic index (PI) (determined by the average number of phagocytized microbes with one active leukocyte), phagocytic number (PS) (determined by dividing the number of phagocytosed bacteria for the total number of counted leukocytes (100), low and medium molecular weight substances (VNSSM) on red blood cells and blood plasma (determined by M.Ya. Malakhova), citric acid (determined by D.P. Ufimkin and D. N. Kovalenko), erythrocyte sorption capacity (SSE) (determined by A.A. Togaibaev et al.), Β-ketones (beta-hydroxybutyrate) (determined using a glucometer and FreeStyle Optium test strips, manufactured by Abbott Diabetes Care Ltd. ), the coefficient of calculation of endogenous intoxication (KREI) (determined by the formula KREI = ∑VNSMMplazma / ∑В SMMerythrocytes), endogenous load coefficient (CEH) (according to the formula KEN = (VNSMMplasma + VNSMMerythrocytes) × KREI), total blood serum protein (OBS), protein fractions, total cholesterol, cholesterol in low density lipoproteins (HDL), cholesterol in low density lipoproteins (LDL), density (LDL), cholesterol in very low density lipoproteins (VLDL), total triglycerides, immunoglobulins A, M, G (determined by the method of biophysical acoustics on the analyzer AKBA-01 "BIOM"), creatinine (determined by the Yaffe method "at the end point" with deproteinization with application ready-made kits of Vital Development Corporation OJSC), urea (determined using Erba-Lachema sro ready-made kits), zinc (determined by the colorimetric method without deproteinization using ready-made kits of Vital Development Corporation OJSC), copper (determined by reaction with batocuproin with using ready-made kits PLIVA-Lachema Diagnostika), inorganic phosphorus (determined by the molybdate uv method using ready-made kits of Vital Development Corporation), bactericidal activity of blood serum (ALS) (determined by O.V. Smirnova and T.A. Kuzmina), lysozyme activity of blood serum (LAS) (determined by V.G. Dorofeychuk). The course of the prenatal, birth and postpartum periods was evaluated according to the results of clinical and gynecological examinations. The results are presented in tables 1, 2, 3, 4, 5, 6, 7, 8.

As can be seen from table 1, 30-35 days before calving, the content of BINF in the blood plasma increased in the experimental group by 10.9% (p> 0.05). There was no statistically significant difference in the values of this indicator between the experimental and control groups in the first and second blood tests. For 10-14 days after calving, the level of VNSMM in the blood plasma of the cows of the experimental group decreased relative to the dry period by 7.2% (p> 0.05), while in the control it remained almost at the same level. Moreover, in cows of the control group, there was a statistically significant increase in the level of VNSMM in plasma in the dry and postpartum periods compared with the start.

Indicators of VNSMM on erythrocytes in cows of the experimental group at the start, in the dry and postpartum periods were not significantly changed. In the control group, the studied parameter for 65-68 and 30-35 days before birth was at approximately the same level, and after calving, the animals showed an increase of 12.1% (p≤0.05). In the postpartum period, the content of VHFMS on erythrocytes in the cows of the experimental group was reduced relative to the control by 8.8% (p≤0.01), which indicated a decrease in the endogenous toxic load on the body of the cows due to the redistribution of endogenous toxins in the blood between plasma and uniform elements as a result of an increase in the ability of red blood cells to sorb under the influence of endotoxins.

30-35 days prior to calving, CREI and CEN increased compared to the start-up period in animals of both groups. Moreover, a more dynamic increase was observed in cows of the control group. In the postpartum period, a decrease in these indicators in experimental and control animals was noted. For 10-14 days after calving, a statistically significant difference in the values of CES at the intergroup level was not established. At the same time, the CREC in animals of the experimental group exceeded that in the control by 5.6% (p≤0.05).

As can be seen from table 2, the content of creatinine in the blood serum of experimental cows in all studies was increased relative to the physiological norm by 26.9-85.0%. After calving, there was a decrease in the creatinine content in cows of both the experimental and control groups by 27.0 (p≤0.05) and 21.7% (p≤0.01), respectively, relative to the previous study, resulting in a difference in the values of the considered indicator by 10-14 days after birth at the intergroup level was 7.5% (p> 0.05). It is known that creatinine is a product of normal metabolism, but in abnormally high concentrations it can cause endointoxication [8], therefore, a more dynamic decrease in its content in animals of the experimental group confirmed the effectiveness of fumaric, ascorbic, succinic and citric acids to optimize one of the indicators of intoxication.

The urea content of cows in the experimental groups at the start, dry period and the postpartum period was reduced relative to the physiological norm by 27.3-57.6%, which indicated a lack of protein in the diet and impaired urea function of the liver. 30-35 days before the birth, a decrease in the level of urea in the blood serum of cows of the experimental and control groups was noted by 36.4 (p≤0.05) and 33.3% (p≤0.01), respectively, and after calving, it increased concentration by 64.3 (p≤0.05) and 18.8% (p> 0.05), respectively, but its indicators remained below standard values. In animals of the experimental group 10-14 days after calving, the urea content exceeded that in the control by 21.1% (p> 0.05).

The sorption capacity of erythrocytes in cows of the experimental group did not undergo significant changes in the dynamics. In animals of the control group after calving, an increase in the test parameter by 4.1 (p> 0.05) and 4.5% (p≤0.05) was noted compared with the periods of start and dead wood. On 10-14 days after the birth, the sorption capacity of erythrocytes in the cows of the experimental group was reduced relative to the control by 5.1% (p≤0.05). The tendency to increase the sorption capacity of red blood cells in animals of the control group was explained by the presence of inflammatory processes in the animal organism and (or) metabolic disturbance.

As can be seen from table 3, the content of β-ketones in the cows of the experimental and control groups for 60-65 and 30-35 days before calving, on average, corresponded to normal values. During the start-up period (60-65 days before calving), the studied indicator in the experimental group was lower than in the control group by 18.3% (p> 0.05). 30-35 days before calving, a decrease in β-hydroxybutyrate was observed in animals of the experimental group by 12.2% (p> 0.05), and in cows of the control group, an increase of 5.0% (p> 0.05). A month before calving, the level of β-ketones in animals of the control group was lower than in the control by 31.7% (p> 0.05). 10-15 days before calving, the level of β-hydroxybutyrate in experimental cows exceeded normal values by 46.7-51.7% and did not have statistically significant differences at the intergroup level. On the 10th day of the postpartum period, a decrease in the content of β-ketones in whole blood was observed in animals of the experimental group by 29.5% (p> 0.05), with an increase in the test parameter in the control by 11.0% (p> 0.05) by compared with the antenatal period. After calving, the concentration of β-hydroxybutyrate in experimental cows decreased relative to control animals by 38.6% (p≤0.05) and on average corresponded to the physiological norm, which indicated optimization of the level of beta-ketones in the blood and, consequently, a decrease in the level of endogenous intoxication .

As can be seen from table 4, more preferred is the dynamics of the content of cholesterol in LDL and VLDL in cows of the experimental group. Thus, the level of total cholesterol in animals of the experimental groups at the start, dry period and postpartum periods corresponded to the physiological norm. The cholesterol content in the cows of the experimental group 30-35 days before birth decreased relative to the start-up period by 21.6% (p> 0.05), which is due to the increased hydroxylation processes for the synthesis of hormones. After calving, there was a further decrease in the test indicator in the cows of the experimental group by 6.9% (p> 0.05). In cows of the control group, the level of cholesterol in the dynamics did not undergo significant fluctuations. For 10-14 days after birth, the cholesterol content in animals of the experimental group was reduced relative to the values of the control group by 15.6% (p> 0.05). The cholesterol content in high-density lipoproteins in animals of the experimental group did not undergo statistically significant changes during the study periods, and in the control for 30-35 days, it decreased by 7.1% (p≤0.05). In this case, statistically significant differences between the groups in the start, dead and postpartum periods have not been established. The concentration of cholesterol in low-density lipoproteins at the start, in the dry and postpartum periods in the cows of the control group did not change and amounted to 1.4 mM / L. In the experimental group, a decrease in this indicator in the dry and postpartum periods was recorded by 36.8 (p≤0.05) and 47.4% (p≤0.05), respectively, compared with the launch. For 10-14 days after calving, the LDL cholesterol content in the cows of the experimental group was reduced relative to that in the control by 28.6% (p> 0.05). In the start-up and dry periods, the cholesterol content in very low-density lipoproteins in the cows of the experimental group was identical and was at the level of 0.4 mM / L, and in the postpartum period, it decreased by 25.0% (p> 0.05). In animals of the control group, 30-35 days before calving, a decrease in the test parameter by 25.0% (p≤0.01) relative to the start period was noted, and after calving, its increase by 25.0% (p≤0.01) by compared with the dry period. In the postpartum period, the concentration of cholesterol in lipoproteins of very low density in the cows of the experimental group was lower than the control by 40.0% (p≤0.01).

The content of total triglycerides in animals of the control group in the dry period decreased relative to the start by 20.0% (p≤0.05), and in the experimental group remained unchanged. 30-35 days before calving, there was no statistically significant difference in the lipid content at the intergroup level. After calving, animals in the experimental group experienced a decrease in their level by 22.2% (p> 0.05), and in the control group, an increase of 37.5% (p≤0.01) relative to the prenatal period. By 10-14 days after birth, the level of total triglycerides in animals of the experimental group decreased by 36.4% compared to control cows (p≤0.01). A decrease in the content of total lipids and cholesterol in the early postpartum period indicated an increase in the intensity of their hydroxylation processes as a result of a sharp increase in the synthesis of estrogens, corticosteroids and other biologically active substances necessary for the normal course of involutional processes after calving

As can be seen from table 5, 30-35 days before calving in cows of the experimental group, FAN exceeded the values of control animals by 15.6% (p≤0.001). After calving, there was an increase in the test indicator in the experimental group by 9.6% (p≤0.05) with a slight change in the control group relative to the dry period. On 10-14 days after giving birth, FAN in animals of the experimental group exceeded that in the control by 24.3% (p≤0.001). PI increased in the experimental group by 4.3 (p> 0.05) and 4.2% (p> 0.05) in the dry and postpartum periods, respectively. In the control group of cows, the FI indicators in the dry and postpartum periods remained practically unchanged and were statistically significantly reduced relative to the start. PHI in animals of the experimental group 30-35 days before and 10-14 days after calving exceeded the cows of the control group by 14.3 (p≤0.001) and 22.0% (p≤0.001), respectively. 30-35 days before the birth, the PS index in the cows of the experimental group increased by 8.6% (p≤0.05) compared to the start-up period, and decreased by 17.1% (p≤0.05) in the control group. In the dry period, the PS in animals of the experimental group exceeded the values in the control by 31.0% (p≤0.01). In the postpartum period, a further increase in PS was observed in animals of the experimental group, while in the control group it remained at the same level. Cows of the experimental group were 10–14 days after giving birth to cows of the experimental group higher than that in the control by 53.6% (p≤0.001).

As can be seen from table 6, 30-35 days before calving, the IgG content increased in the cows of the experimental group by 5.4% (p> 0.05) with a decrease in the control by 13.3% (p> 0.05) relative to the period launch. There were no statistically significant differences in the concentration of IgG in the dry period at the intergroup level. In the postpartum period, the reverse dynamics of the studied parameter was observed: its decrease in the cows of the experimental group by 6.1% (p> 0.05) and an increase in the control by 14.1% (p> 0.05). In the postpartum period, there were no statistically significant differences in IgG content in cows of the experimental and control groups.

The IgA content in animals of the experimental group in the dry and postpartum periods was identical and exceeded the values during the start-up period by 4.0% (p> 0.05). 30-35 days before the birth, the cows of the control group showed a decrease in IgA by 17.9% (p≤0.05), and for 10-14 days there was an increase in the studied parameter by 13.0% (p> 0.05) - to the same level with the experimental group.

30-35 days before and 10-14 days after calving, the IgM content in the cows of the experimental group was identical and was at the level of 1.5 mg / ml, which is 15.4% (p> 0.05) higher than in launch period. In the cows of the control group, in the dry period there was a decrease in IgM level by 25.0% (p≤0.05) compared with the previous study, and after calving - an increase of 16.7% (p> 0.05). On 10-14 days after calving, a statistically significant difference in the values of the studied indicator between the experimental and control groups was not established. The total value of the three studied classes of immunoglobulins during the launch period in experimental cows was reduced relative to control animals by 7.7%. In the dry period, a decrease of the studied parameter in the control group by 15.5% and an increase in the experimental group by 6.1% were recorded. 30-35 days before the birth, the sum of the immunoglobulins G, A, M in the cows of the experimental group exceeded the control value by 15.8%. In the postpartum period, the reverse dynamics was observed, namely: an increase in the values of the studied parameter in the control by 14.2% and a decrease in the experimental group by 4.3%.

As can be seen from table 7, the level of obstetric morbidity in experimental cows was 44.1% lower than in the control group. At the same time, the retention rate of the placenta decreased by 22.5% (p≤0.05), the incidence of endometritis by 31.2% (p≤0.01), ovarian pathology - by 22.4% compared with the control. In the cows of the experimental group, ovarian cysts were not noted, a decrease in the incidence of hypofunction by 17.8% (p> 0.05) in comparison with control animals.

As can be seen from table 8, in the experimental group there was a statistically significant reduction in the timing of involution of the reproductive apparatus after childbirth and the duration of infertility by 5.3 and 20.1 days, respectively. In the experimental group, 90.5% of animals were inseminated, which is 4.1% (p> 0.05) more than in the control group. In the control group, there was a decrease in cow fertility by 10.6% (p> 0.05) with a decrease in the effectiveness of the first insemination by 31.7% (p> 0.05). The cow fertilization index (the rate of insemination per fertilization) in the experimental group was lower than the control group by 15.0% (p> 0.05).

The results of the studies confirmed that when implementing the claimed combination of essential features, a technical result is achieved - reducing the risk of developing inflammatory diseases of the uterus in cows. Thus, the level of obstetric diseases decreased by 44.1% (retention of the placenta by 22.5%, endometritis by 31.2%), while the prophylactic effectiveness of ovarian dysfunctions increased by 21.7% (p> 0.05) compared with control. The effectiveness of the claimed method is ensured by correction of the state of immunobiochemical homeostasis and optimization of the level of endogenous intoxication. So, in cows of the experimental group, the phagocytic number increased by 9.6%, the phagocytic activity of neutrophils and the phagocytic index were maintained at an optimal level, the level of cholesterol in low-density lipoproteins in the dry period decreased by 36.8% (p≤0.05) , the optimal values of VNSMM (RBC), sorption ability of erythrocytes, β-hydroxybutyrate, cholesterol in very low density lipoproteins, total triglycerides, phagocytic index and phagocytic number after calving were preserved.

Example 2. According to the analogue principle, three groups of dead cows were formed 65-68 days before calving: experimental (n = 33) and two control (n = 27 and n = 30). Cows of the experimental and first control groups were injected intramuscularly with a 0.5% sodium selenite solution intramuscularly once 20-25 days before calving, tetravit was injected intramuscularly once every 10 days 20 and 25 days before calving, and cows of the experimental group 1 once a day for 65 days before and 10 days after calving, fumaric, ascorbic, succinic and citric acids were orally administered per dose, mg / kg body weight - fumaric acid - 2.0; ascorbic acid - 3.0; succinic acid - 4.0; citric acid - 1.0 Cows of the second control group of drugs were not used. On 10-14 days after calving of the experimental animals, the level of VNSMM on erythrocytes and in blood plasma was determined. The animals were clinically observed, noted the nature of the course of the prenatal, birth and postpartum periods, performed clinical and gynecological examinations of cows, took into account the number of diseased animals, the timing of involution of reproductive organs, hunting and insemination. The results are presented in table 9.

As can be seen from table 9, the introduction of organic acids into the prevention scheme in accordance with the claimed method provided a significant increase in the main indicators. So, after calving, 9.1% of cows fell ill in the experimental group, and 37.0% in the first control group. The decrease in the incidence of cows in the experimental group had a positive effect on their reproductive ability. So, the terms of genital involution were 9.9 days shorter than in cows from the control group, and the period from calving to fruitful insemination was 19 days shorter. Indicators of endogenous intoxication (10-14 days after calving): experimental group - VNSMM (RBC), units 32.5 ± 1.6; VNSSMM (pl.), Unit 13.2 ± 0.5; control group - VNSSMM (RBC), units 37.6 ± 0.7: VNSMM (pl.), Unit 14.0 ± 0.4.

Example 3. According to the principle of analogue pairs, two groups of deep-skinned cows were formed 60 days before the expected calving, who were in the same conditions of keeping and feeding (experimental and control 25 animals each). Table 10 presents the scheme of experience.

On 10-14 days after calving of the experimental animals, the level of VNSMM on erythrocytes and in blood plasma was determined. The animals were observed, noted the nature of the course of the prenatal, childbirth and the postpartum periods, performed clinical and gynecological examinations of cows, took into account the number of diseased animals, the timing of involution of reproductive organs, hunting and insemination. The results are presented in table 11.

Indicators of endogenous intoxication (10-14 days after calving): experimental group - VNSMM (RBC), units 30.7 ± 1.7; VNSSMM (pl.), Unit 13.3 ± 0.6; control group - VNSSMM (RBC), units 36.6 ± 2.2; VNSSMM (pl.), Unit 14.0 ± 0.4.

Example 4. According to the principle of analogue pairs, two groups of deep-skinned cows were formed 60 days before the expected calving, who were in the same conditions of keeping and feeding. Table 12 presents the scheme of experience.

On 10-14 days after calving of the experimental animals, the level of VNSMM on erythrocytes and in blood plasma was determined. The animals were observed, noted the nature of the course of the prenatal, childbirth and the postpartum periods, performed clinical and gynecological examinations of cows, took into account the number of diseased animals, the timing of involution of reproductive organs, hunting and insemination. The results are presented in table 13.

Indicators of endogenous intoxication (10-14 days after calving): experimental group - VNSMM (RBC), units 36.2 ± 2.2; VNSSMM (pl.), Unit 14.1 ± 1.3; control group - VNSSMM (RBC), units 40.7 ± 3.2; VNSSMM (pl.), Unit 14.6 ± 1.0.

The obtained data confirmed the feasibility of the method and the achievement of a technical result — a reduction in the risk of developing postpartum inflammatory uterine diseases in cows using fumaric, ascorbic, succinic, and citric acids in a mixture in a dose and according to a scheme that ensures the maintenance of homeostasis and increases the adaptive potential by reducing the level of endogenous intoxication. The optimization of indicators of the level of endogenous intoxication, integral markers of the state of metabolic processes and immunological reactivity in the dry and postpartum periods when using the proposed method provided a statistically significant decrease in the level of obstetric diseases.

Information sources

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Claims (1)

A method of reducing the risk of developing postpartum inflammatory diseases of the uterus in cows, comprising administering biologically active substances of antioxidant action, characterized in that fumaric, ascorbic, succinic and citric acids are orally administered to cows once a day for 65 days before and 10 days after calving. based on the dose, mg / kg body weight - fumaric acid - 2.0; ascorbic acid - 3.0; succinic acid - 4.0; citric acid - 1.0.