RU2617300C1 - Method of influencing ratio of sexes in offspring of laboratory rats - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to biology, namely to method of influencing ratio of sexes in offspring of laboratory rats. Method includes keeping animals of different sexes separately for 12 days. 2.0% water solution of sodium hydrocarbonate in dose 5 ml/kg of weight is introduced to animals perorally with 12 hour interval, after which male and female animals are mated and the course is continued for 5 days, after which females are separated from males, and offspring is expected.

EFFECT: application of the invention will make it possible to increase birthrate of male individuals.

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Description

The invention relates to biology and can be used to change the sex ratio in the offspring of laboratory animals.

A known method of regulating the birth of predominantly female rabbits, based on the selective damage of the semispecific sperm (C y chromosome) by the immune serum to the H-Y antigen, after 8-fold immunization of female rabbits with antigen prepared from the spleen of a male rabbit. In this case, 66% of females and 34% of males receive progeny in the offspring (Erokhin A.S., Zhiltsova L.S. Sperm release from dead sperm and microorganisms and isolation of a fraction of sex-determining sperm / Selection and genetic methods for increasing the productivity of agricultural animals: Sat scientific research / Gosagroprom of the USSR, VNPO on livestock breeding, Golovn selection - genetic center - Moscow: 1987, p. 93).

The disadvantages of this method are: the need to attract large material, technical, labor and financial resources. The method is not sufficiently developed, which complicates its application in practice.

The closest in purpose and technical essence to the claimed method is a method of shifting the sex ratio in a population of laboratory mice (Patent No. 1337018 from 09/15/1987) by exposing male mice to an electric field with a voltage of 45-50 kV / m for 5-7 hours every day within 12-15 days. Then females are planted with males and exposed to all animals with an electric field in the same mode until offspring are received from them.

The disadvantages of this method is the complexity of its reproduction and high cost (requires special equipment). Moreover, the use of voltage of 45-50 kV / m has a negative impact on the health of both the experimenter and animals. In experiments, males are more often used, which excludes the hormonal effect associated with the estrous cycle in females.

As a result, there is a need for more males for laboratory research.

The purpose of the invention is the provision of increasing the birth rate of males.

The essence of the invention lies in the fact that animals are orally administered with an aqueous 2.0% solution of sodium bicarbonate, obtained by dissolving 2 g of NaHCO3 in 100 ml of distilled water. The introduction of the solution is carried out every 12 hours at the rate of 5.0 ml / kg weight for 12 days before mating and 5 days after the joint content of males and females.

This method leads to the birth of 75% of males, i.e. 25% more than in control.

Practical application is as follows.

To obtain a solution, 2 g of sodium bicarbonate NaHCO3 is dissolved in 100 ml of distilled water with a temperature of 50-60 ° C, then cooled to room temperature. To Wistar rats weighing 200-250 g body weight every 12 hours, a solution is administered orally at the rate of 5.0 ml / kg body weight for 12 days. All this time, males and females are kept separately. After 12 days, the females are planted in the males and the sodium bicarbonate solution is continued to be administered for 5 days every 12 hours. After 5 days, the animals are divided by sex until the offspring.

Example

To eliminate the influence of possible external factors, experiments were carried out in winter and summer in the same feeding and keeping conditions. There were no significant differences depending on the time of year. Groups of animals were formed by sex of 10 females and 10 males. The control group also consisted of 10 males and 10 females and was in the same conditions of feeding and maintenance as the experimental one. After a 12-day course of oral administration of a solution of sodium bicarbonate at a dose of 5.0 ml / kg with an interval of 12 hours in the experimental group and distilled water in the control group at a similar dose, the females were transferred to males and continued to inject the solution without changing the dose for another 5 days. After this period, the females were transferred from males to individual cells. The resulting litter was the ratio of males 75% and females 25% in the experimental group and 48-52% in the control group, respectively.

The sex ratio in the offspring has changed in comparison with the control group by 25%, based on this, we can conclude about the effect of sodium bicarbonate on the sex ratio in the offspring of laboratory animals.

Claims (1)

A method of influencing the sex ratio in the offspring of laboratory rats, including heterosexual keeping of animals for 12 days, characterized in that the animals are orally administered with a 2.0% aqueous solution of sodium hydrogen carbonate at a dose of 5 ml / kg of body weight, then males and the females mate and continue the course for 5 days, after which the females are separated from the males and the offspring are expected.