RU2617235C1 - Detection method of african swine fever infectious agent in the remote nidus of infection until its natural incidental presentation (versions) - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: to detect the infectious agent ASF in the nidus of infection until its natural incidental presentation define the coordinates of dominant height of the ground surface natural elevation with respect to the sea level. Then provide intravital sampling of domestic pigs and wild boars blood, as well as the pathological material from fallen domestic pigs and wild boars, sampling from pig products, and sampling of water and soil in the potentially troublesome area, followed by the diagnosis of the samples taken. Initially determine the coordinates of at least one dominant height of the natural elevation on the potentially troublesome area. The dominant height is chosen with the excess of 3 meters relatively to the other heights in the investigated area. The area radius of the investigated adjacent territory is chosen within 20 km. The water and soil sampling is carried out within the radius of 100 meters around the border, adjacent to the dominant height of the territory. The samples diagnosis is carried out using PCR hemoadsorption or immunofluorescence.

EFFECT: invention provides early detection of african swine fever virus and allows to prevent its further spread.

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Description

The present invention relates to veterinary medicine and can be used to prevent the spread of African swine fever, both among domestic livestock of pigs and among wild boars.

It is known that the occurrence of epizootics among animals can lead to significant economic damage and cause severe shock to the state and society. Moreover, the development of methods for the rapid detection and elimination of foci of infection before the natural incidental manifestation of the virus is particularly relevant in relation to the specific natural geological and geographical conditions of threatened territories.

African swine fever (ASF) is a highly contagious viral disease. Mortality, as a rule, is 100%. The causative agent of the disease (infectious agent) is a DNA-containing virus. Sources of the virus are domestic pigs and feral pigs with African plague. The virus can be transmitted with the products of slaughter of sick pigs, infected feed, water, not neutralized food and slaughter waste, as well as contaminated bedding. Carriers can be blood-sucking insects, ticks, as well as maintenance personnel. There is no vaccine against ASF. Control measures are based on the detection of infection (virus and / or specific antibodies) using laboratory methods, the destruction of all pigs in the ASF area, disinfection and quarantine, the establishment of the 1st (5-20 km) and 2nd (up to 200 km) threatened zones (official site of the Rosselkhoznadzor, fsvps.ru).

Currently, the situation in the Russian Federation for ASF is endemic (illustrating Fig. 1, Fig. 2). In total, more than 685 cases of diseases among domestic pigs and wild boars were registered in the Russian Federation [1]. More than 30 billion rubles have already been spent on the fight against ASF in Russia.

The appearance of ASF outside the endemic zone leads to high mortality (up to 100%) of pigs in the primary foci and the formation of natural foci [2]. Skidding and spreading of ASF can occur with domestic pigs, especially on private farms, with wild boars, as well as on highways with pig products, food waste and mechanically when moving the population and goods. In this case, the disease spreads at a high speed due to foci of infection, which are formed by linearly radiative movement of the infection from the primary focus along transport streams and / or ways of moving wild boars. The spread of the virus from the resulting focus, as a rule, occurs in a diffuse way and depends largely on the population density of domestic pigs and / or wild boars. Foci of infection can appear many thousands of kilometers from the primary focus, for example, due to the movement of infection using air, land or water transport in places of concentration and rotation of people, transport, cargo.

Until now, there was no reliable advance way to determine the most probable place of occurrence of the source of infection in relation to specific natural geological and geographical conditions of threatened territories. This is largely due to the fact that the registration of such foci, as a rule, is incidental in nature, when the disease has already clearly manifested itself and its diffuse spread has begun. Identification of the foci of infection can also be carried out by total monitoring of the epizootic situation among the stock of animals susceptible to ASF disease or of the moved pig production, which is not efficient enough and requires significant costs.

The existing Instruction [3] and the Rules [4, 5] do not provide for proactive technical measures for the early detection of an infectious agent.

In the event of an epizootic focus of ASF infection, measures are taken to eliminate the infection and prevent its further spread [3, 4, 5]. At the same time, to declare the territory of the economy, settlement or region unsafe according to ASF, establish quarantine in them and organize the necessary measures for the prevention and elimination of the disease, the boundaries of the epizootic focus and the boundaries of the first and second threatened zones are determined.

In this case, the first threatened zone is considered to be the territory directly adjacent to the epizootic focus of ASF to a depth of 5 to 20 km from its borders, taking into account economic, commercial and other ties between settlements, farms and the epizootic focus. The second threatened zone is considered to be the territory surrounding the first threatened zone, with a depth of 100-150 km from the epizootic focus. Further, a set of technical measures are taken at quarantined sites and territories to eliminate the ASF focus, aimed at detecting and destroying a pathogenic agent, and preventing its spread. At the same time, the indicated technical measures are carried out within the boundaries of the most-at-risk areas, established only when an epizootic focus of ASF associated with the appearance of a pathogenic agent appears.

To date, there has been no reliable advance determination of local areas of greatest risk arising from foci of infection. It is known, for example [6], that risk analysis includes hazard identification, risk assessment, risk management and risk communication. However, existing risk assessment techniques, including spatial-geographical analysis, using the Moran autocorrelation method [7], do not allow reliable identification of risk zones in terms of foci of infection and the spread of infection and technical measures to detect and localize the infection well in advance of the natural manifestation of infection .

Therefore, the largely known [3, 4, 5] actions to prevent the spread of the ASF virus are based mainly on the implementation of appropriate technical measures through management measures based on mutual agreement on the implementation of the relevant rules of conduct by those responsible for their observance. At the same time, the effectiveness of technical measures aimed at detecting and destroying the ASF virus and preventing its spread in modern conditions of rapidly developing economic relations is insufficient. In connection with the foregoing, technical measures carried out in order to detect the ASF virus must be separated from the economic relations and transferred to the technical sequence of actions on tangible objects aimed at detecting an infectious agent in the foci of infection of African swine fever before its natural incident manifestation, which will ensure localization of the focus of infection in a short time. To carry out a specific list of technical measures to prevent the spread of ASF, it is necessary to identify and use a permanently existing and independent of economic relations permanent physical attractor that determines the spread of ASF virus by the linear beam method with the formation of foci of infection. A permanent physical attractor is the terrain, which has a geotectonic basis and predetermines the behavior of biological objects on the surface of the earth for many millennia. Typically, the area of greatest risk coincides with the place of concentration and rotation of people, vehicles and animals, including those susceptible to disease.

As a result of studies [8] conducted in 2008, the characteristics of the spread of especially dangerous diseases in the Belgorod Region, it was found that pathogenic agents, like other biological objects (people, wild animals), tend to concentrate on territories associated with tectonic uplifts with dominant relative to sea level natural heights of the earth's surface. Thus, the greatest probability of the occurrence of infectious diseases can be in places of concentration and rotation of the population, domestic and wild animals, confined to the highest heights of tectonic uplifts and the natural heights of the earth's surface.

The peculiarity of the behavior of biological objects during their life cycles, including when moving around the area, was also confirmed as a result of numerous observations over 18 years of the behavior of wild ungulates, including wild boar. In this case, wild animals in the vast majority of cases when moving around the area passed through the dominant heights or remained for a day near the dominant heights, or arranged their den near the dominant heights. Thus, their life cycles were directly related to the heights dominating in a certain territory. It was found that in the territories confined to tectonic uplifts dominating relative to the sea level and the natural heights of the earth’s surface, the most probable energetically determined movement, concentration and rotation of people and domestic animals in limited territories took place (the “bottleneck effect” arose). Moreover, this is the desire of biological objects to visit territories adjacent to the heights that dominate in a certain territory, that is, a kind of tunneling (inversion of the “tunneling effect” known in quantum physics) is manifested as large (10-30 thousand km2 or more), and small (1-5 km sq.) territories, for example, individual hunting farms. In fact, moving along watersheds reaching geographic heights is more energy-efficient in terms of energy conservation than walking across them. Therefore, the occurrence of ASF as the result of the formation of foci of infection in new territories confined to the dominant heights of the earth’s surface by a person using a linear beam path is most probable, which is associated with traffic flowing, as a rule, along the watersheds, and possible concentration of the population or crossing its pathways , first of all, from infected (threatened) territories.

For example, according to a 2008 study (8), the most likely primary threatened territories for ASF penetration into the Belgorod region were the Prokhorov-Ivnyansky and Biryuchansky sections (zones 1, 2 and 3, respectively, in Fig. 3), confined to the dominant heights of tectonic uplifts . And already in 2013 in with. Livenka of the Krasnogvardesky (Biryuchansky) district of the Belgorod region (zone 2 of Fig. 3) for the first time in the Belgorod region arose a source of ASF infection. Thus, the forecast was justified. In 2014, also in the Gubkinsky district of the Belgorod region, a source of ASF infection appeared in zone 3 of FIG. 3, confined to an altitude of 274.5 meters above the ocean level that dominates the entire territory of the region. In addition, in 2011, adjacent to zone 1 of FIG. In the territory of the Kursk region, a source of ASF infection also arose. A good example confirming the revealed pattern of ASF virus concentration in territories adjacent to the dominant heights is the formation of the Unified Noozoareal “West of the Russian Federation” during 2007 ... 2015 [1] (Fig. 1).

From FIG. 1 it can be seen that the single nosoareal “West of the Russian Federation” includes the “Southern” endemic zone and the “Northern” endemic zone. At the same time, the Caucasus Mountains were the attractors contributing to the formation of the “Southern” endemic zone, and the Valdai Upland for the “Northern” endemic zone, where in the territory confined to it in the Tver region in 2011 the first foci of ASF infection appeared (Fig. 2, BUT). Further, as a result of the diffuse spread of the virus, the disease literally within three years covered a significant area, and a large “Northern” endemic zone of FIG. 2, B. Wild animals, including wild boars, also moving along watercourses, including riverbeds, fall on hills and then move along other watercourses originating on these hills.

Biological objects tend to move around the territory with minimal energy consumption. This is manifested not only as a result of the choice of the path of movement, but also when the biological object is located in the places of greatest information and biological safety, due to the predominant energy state, due to being at a dominant height. When located in such territories, the predominant energy state can be explained by the action of ordinary physical laws. For example, for wild animals, when finding them at dominant heights, the advantage is manifested in that; that animals receive the greatest amount of communicative information (smells, sounds, visibility, etc.), allowing them to ensure the safe (minimum energy expenditure) implementation of their life cycles, thereby protecting their genetic information with minimal energy expenditure. Thus, biological objects when moving, mainly choose the path to these territories, due to the lowest energy costs. Moreover, during ground movement, usually, the specified path passes along the ridges, caused, for example, by anticlises, through a series of uplifts or heights dominating in a certain territory. Thus, the part of the potentially threatened territory where the outbreak is most likely to occur can be determined in advance by identifying the infectious agent in advance of its natural occurrence.

The objective of the invention is to provide a method for the early detection of an infectious agent in a site of infection of ASF before its natural incident manifestation, taking into account the terrain of specific areas in which there are or may be animals susceptible to the disease (domestic pig and / or wild boars), which allows the most rapid preventive measures, including localizing the source of infection and preventing the spread of infection.

The problem is solved by identifying a potentially threatened territory, determining the dominant height in this territory, taking blood samples from domestic pigs and wild boars, material from dead animals, samples from pig products, water and soil in a potentially threatened area, followed by diagnosis of the samples taken, at the same time, sampling is carried out on a site of a potentially threatened territory adjacent to the height that dominates in this territory, which is determined before the indicated action.

The essence of the proposed invention is the linking of the preventive detection of ASFV virus to the landscape of the territory of movement of biological objects, based on the physical laws of the movement of these objects and on the use of the properties of biological objects to concentrate under the action of a geotectonic attractor, which is the topography. A method for identifying an infectious agent in the infection site prior to its natural incident occurrence is to first determine the coordinates of the dominant height of the natural elevation of the earth’s surface relative to the ocean level in a potentially threatened area, then conduct in vivo sampling of blood from domestic pigs and wild boars, as well as material from fallen domestic pigs, wild boars, sampling from pig products, water and soil samples, with subsequent diagnosis of the samples taken, while The above-mentioned actions to detect an infectious agent are primarily carried out on parts of a potentially threatened territory adjacent to the height dominating in this territory. The dominant altitude is chosen with an excess of more than 3 meters relative to other altitudes located on a potentially threatened territory. The coordinates of the dominant altitude are determined using known means, for example, using cartographic material having elevation coordinates or using space observation and / or positioning tools. A part of the potentially threatened territory adjacent to the dominant height is chosen within a radius of 20 kilometers from the dominant height, while water and soil sampling is carried out along the borders of the part of the territory adjacent to the dominant height in places of natural watercourses and in a radius of up to 100 meters around suffusion depressions nature, located on land plots within this territory, and sampling from pig products is carried out along the borders of the territory adjacent to the dominant height at the points of intersection of their transport routes.

The technical result from the use of the proposed method is the early detection of an infectious agent in the site of infection of African swine fever (ASF) to its natural incident occurrence, which allows preventive measures to be taken in advance, including the destruction of the ASF virus in it, and thereby prevent the spread of ASF.

The invention is illustrated graphic materials.

In FIG. 1 schematically depicts the epizootic situation of African swine fever in edemic zones on the territory of the Russian Federation (2007-2015) and the formation of a single nosoareal [1];

in FIG. 2 shows a pattern of the development of an epizootic situation in the Russian Federation and the formation of epizootic zones for the periods 2007-2011, A and 2007-2014, B [1];

in FIG. 3 depicts schematically the epizootic situation of anthrax in the territory of the Belgorod region for the period 1936-1985; The concentration of foci of domestic anthrax diseases of animals and adjacent cattle burial grounds for the period from 1936 to 1985, where 1 and 2, respectively, Prokhorov-Ivnyansky and Biryuchansky foci of disease; 3 - the territory adjacent to the highest altitude (274.5 meters above sea level) Gubkinsky district of the Belgorod region;

in FIG. 4 shows a diagram of the implementation of the claimed method according to one of the preferred options, where in the depicted potentially threatened area 4 square dots and three-digit numbers are indicated in meters of the height of the earth's surface above sea level;

in FIG. 5 shows a diagram of the implementation of the claimed method according to another of the preferred options, where in the depicted potentially threatened area 11 square dots and three-digit numbers are indicated in meters of the height of the earth's surface above sea level, and the dominant heights are indicated by dark triangles.

There is one of the preferred options, a method for detecting an infectious agent by African swine fever in the foci of infection before its natural incidence, which includes: intravital blood sampling from domestic pigs, wild boars, material from dead domestic pigs, wild boars, from pig products, and also water and soil sampling in potentially endangered areas and diagnostics of samples taken. In accordance with the proposed variant of this method, the coordinates of the dominant height of the natural elevation of the earth’s surface relative to the sea level are first determined on the potentially threatened territory, and the above-mentioned actions to detect the infectious agent are primarily carried out on the part of the potentially threatened territory adjacent to the height dominating in this territory. According to the execution of the method, the dominant altitude is determined with an excess of more than 3 meters relative to other altitudes located on a potentially threatened territory, and the coordinates of the dominant altitude are determined using cartographic material having altitude coordinates or using space observation and / or positioning tools. At the same time, a part of the potentially threatened territory for its investigation adjacent to the dominant altitude is chosen within a radius of 20 kilometers from the dominant altitude.

It is also possible to carry out a variant of the proposed method, in which water, soil samples are taken along the border of the territory adjacent to the dominant height, in places of natural watercourses and in a radius of up to 100 meters around depressions of nature located on plains within this territory, and sampling from Pig breeding products are carried out along the border of the territory adjacent to the dominant height, at the points where it crosses by transport routes.

There is also an option according to which intravital blood sampling from domestic pigs, wild boars, material from dead domestic pigs, wild boars, pig products, as well as water and soil in a potentially threatened area for subsequent diagnosis, is carried out primarily on parts of potentially threatened territory adjacent to dominant heights located on a potentially threatened territory. In accordance with the proposed variant of this method, the coordinates of the dominant heights of the natural elevations of the earth’s surface relative to the sea level are first determined on the potentially threatened territory, and the above-mentioned actions to detect the infectious agent are primarily carried out on parts of the potentially threatened territory adjacent to the selected dominant heights located on the potentially threatened territory. According to another embodiment of the proposed method, the coordinates of the dominant heights are determined using cartographic material having the coordinates of the heights, or by means of space observation and / or positioning.

The proposed variants of a method for detecting an infectious agent of African swine fever in the source of infection before its natural incident manifestation include only technical actions with material objects aimed at detecting a pathogenic agent in a specific area of a potentially threatened territory, depending on a geotectonic attractor. These actions are not based on economic relations, and their result is aimed at achieving a specific technical result, which consists in the early detection of an ASF infectious agent before its natural incident manifestation and in advance of preventive measures, including the destruction of the ASF virus in it, and thereby preventing the spread of ASF.

Examples of specific use of the invention

The following are examples of the method.

In these examples, the diagrams (Figs. 4 and 5) of the respective versions of the variants of the method under consideration are shown. At the same time, the diagrams depict a model potentially threatened territory and information about administrative entities (regions, districts, settlements), business entities, and geographical names of territories are not deliberately reflected. This is due to the fact that the execution of this method is a technical sequence of actions on material objects, united by a single technical concept aimed at detecting an infectious agent of African swine fever before its natural incident manifestation, which can be used in territories of any geographical latitude and longitude.

Example 1

Consider an embodiment of a method for detecting an infectious agent of African swine fever. foci of infection prior to its natural incident occurrence in a potentially threatened area using the circuit depicted in FIG. 4. For example, we choose a potentially threatened territory 4 with an area of 10 thousand square meters. km, which is characteristic of a particular geographical area or part thereof, located, for example, in a subject of the federation.

Moreover, for the implementation of the considered variant of the method on a potentially threatened territory 4, we determine the coordinates of the dominant height of the natural elevation of the earth's surface relative to the sea level, for example, using cartographic material with designations and elevation coordinates.

In this case, the minimum height of the site is 169 meters, and the maximum is 256 meters. In this example, the heights of the earth's surface are indicated, indicated by three-digit numbers, which is typical, for example, for the Central Russian Upland.

However, it should be understood that for the implementation of the method it is important to have a height difference with a dominant height, and not the absolute value of the latter. Moreover, the greater the elevation difference, the more effective the action of the geotectonic attractor.

In this case, a height of 256 meters is dominant over the entire potentially threatened territory 4, and its geographical coordinate, for example, is 51 ° 03 '51.08ʺ s. w. and 36 ° 72 '93.52ʺ East The closest height to it is 220 meters, which is 30 meters less than the dominant height. Next, we determine the area 5 of the potentially threatened territory 4, adjacent to the dominant height of 256 in a radius, for example, about R = 10 km, on which, most likely, a source of ASF infection can occur. Further, using well-known methods, we primarily conduct intravital blood sampling from domestic pigs and wild boars, selection of material from dead domestic pigs and wild boars located in Section 5 of the potentially threatened territory 4. We take water and soil samples along the boundaries of Section 5 potentially threatened territory 4, adjacent to the dominant height of 256 meters, at the intersection of 9 natural watercourses 10 and in a radius of up to 100 meters around depressions of a suffusion nature 7, located on the plains within the area and 5 territories. We take samples from pig breeding along the borders of the territory adjacent to the dominant height of 256 meters, at the intersection of 8 by transport routes 6. Then we diagnose all the samples using known methods, for example, PCR (polymerase chain reaction), hemi-adsorption reactions, reactions direct immunofluorescence.

If the ASF virus is detected in any of the selected samples, measures are taken to eliminate the infection and prevent its further spread in accordance with the current Instructions [3].

Example 2

Consider an embodiment of a method for detecting an infectious agent of African swine fever in a foci of infection prior to its natural incident occurrence in potentially threatened area 11 using the circuit depicted in FIG. 5.

Consider a potentially threatened area of 11 with an area of 22 thousand square meters. km, which is typical for a particular geographical area or subject of the federation.

To implement the considered method variant on a potentially threatened territory 11, first of all, we determine the coordinates of the dominant heights of natural elevations of the earth's surface relative to the sea level, for example, using cartographic material with designations and elevation coordinates.

In FIG. 5, the dominant heights of 226 and 256 meters are indicated by dark triangles. At the same time, the minimum height of the investigated potentially threatened territory 11 is 175 meters. The dominant heights of 256 and 226 meters differ from each other by only 24 meters and are located at a considerable distance from one another. Potentially threatened territory 11 has a large area of 22 thousand square meters. km, while the coordinates are determined for both dominant heights.

For example, a height of 256 has a coordinate of 51 ° 03 '51.08 ʺ s. w. and 36 ° 72 '93.52ʺ East and a height of 226 meters has a coordinate of 50 ° 49 '58.49ʺ s. w. and 38 ° 28 '37.01ʺ East Next, we determine the parts 12 and 13 of the potentially endangered territory 11, adjacent, respectively, to the dominant heights of 256 and 226 meters in a radius, for example, R1 = 15 km and R2 = 15 km, where the most likely source of ASF infection can occur. Next, using well-known methods, we primarily conduct intravital blood sampling from domestic pigs and wild boars, material from dead domestic pigs and wild boars located in parts 12 and 13 of the potentially threatened territory I. We take water and soil samples along the boundaries of parts 12 and 13 potentially threatened territories 11 adjacent to the respective dominant heights of 256 and 226 meters, in places of 14 natural watercourses 16, and also in a radius of up to 100 meters around a decrease in the suffusion nature 18, located on the plac ah inside the territory 12. We take samples from pig breeding products along the borders of sections 11 and 12 of the territories adjacent to the dominant heights of 256 and 226 meters, respectively, at the places of their intersection by transport routes 17. Then we diagnose all the selected samples using known methods, for example , PCR (polymerase chain reaction), hemadsorption reactions, direct immunofluorescence reactions.

If the ASF virus is detected in any of the selected samples, measures are taken to eliminate the infection and prevent its further spread in accordance with the current Instructions [3].

Thus, the proposed method for detecting an infectious agent of African swine fever in the foci of infection before its natural incident manifestation allows one to detect in advance an infectious agent (ASF virus) in the foci of infection of African swine fever (ASF) before its natural incidence in potentially threatened areas, which allows to carry out preventive measures in advance, including with the destruction of the ASF virus in it, and thereby prevent the spread of ASF and application nogo economic damage.

Thus, the problem facing the invention is solved.

The use of this method is especially effective when there is a wild boar population on a potentially threatened territory, since its movement in the natural environment of the habitat is difficult to control.

In addition, it should be noted that the present invention can be used to determine in the foci of infection on potentially threatened territories other infectious agents of diseases dangerous for both animals and humans, for example, highly pathogenic avian influenza, anthrax and other especially dangerous diseases.

Sources used

1. Forecast for African swine fever in the Russian Federation for 2015 / O.N. Petrova, F.I. Korennoy, S.A. Dudnikov, N.S. Bardina, E, e, Tapenko, A.K. Karaulov [et al.]; Federal Vet Service and phytosan. supervision; IAC Office of Veterinary Supervision. - Vladimir: FSBI ARRIAH, 2015. - 34 p. http://www.fsvps.ru/fsvps/iac.

2. African swine fever: Epizootic polymorphism and control. Naturally historical background. Part 1. / V.V. Makarov, V.A. Rough / http://www.fsvps.ru/fsvps/asf/publications/, the official website of the Rosselkhoznadzor.

3. Instruction on measures for the prevention and elimination of African swine fever, approved by the Main Veterinary Directorate of the Ministry of Agriculture of the USSR of November 21, 1980.

4. Veterinary and sanitary rules for the fight against African swine fever, approved. Decree of the Ministry of Agriculture and Food of the Republic of Belarus dated December 22, 2009 No. 84.

5. Draft updated Regulation on the control of African swine fever / www.mcx.ru.

6. OIE Terrestrial Animal Health Code, as amended in 2006.

7. Forecast for African swine fever in the Russian Federation for 2014 / S.A. Dudnikov, O.N. Petrova, A.K. Karaulov [et al.]; Federal Vet Service and phytosan. supervision; FSBI "ARRIAH; IAC Office of Veterinary Supervision - Vladimir, 2014 .-- 42 p.

8. Moskvitin S.A., Sorokin. V.N., Novichenko V.V., Moskvitina U.S. Features of the spread of especially dangerous diseases on the territory of the Belgorod region (by the example of anthrax) // Vestnik Ohotovovedeniya, 2012, Volume 9, No. 2, p. 253-258.

Claims (9)

1. A method for detecting an infectious agent of African swine fever in a foci of infection prior to its natural incident occurrence, characterized in that they first determine the coordinates of at least the dominant height of the natural elevation of the earth’s surface relative to the ocean level in a potentially threatened area, then perform intravital blood sampling from domestic pigs and wild boars, as well as material from fallen domestic pigs and wild boars, sampling from pig products, water and soil samples from subsequent diagnosis of the selected samples, while the sampling is, first of all, carried out on the part of the potentially threatened territory adjacent to the height dominating in this territory. 2. The method according to claim 1, characterized in that the dominant height is selected with an excess of more than 3 meters relative to other heights located on a potentially threatened territory. 3. The method according to p. 1, characterized in that the coordinates of the dominant height is determined using cartographic material having the coordinates of the heights. 4. The method according to p. 1, characterized in that the coordinates of the dominant height is determined using space observation and / or positioning. 5. The method according to p. 1, characterized in that a part of the potentially endangered territory adjacent to the dominant altitude is chosen within a radius of 20 kilometers from the dominant altitude, 6. The method according to p. 1, characterized in that the sampling of water and soil is carried out along the border of the territory adjacent to the dominant height, in places of natural watercourses and in a radius of up to 100 meters around depressions of nature located on plains within this territory, and sampling of pig products is carried out along the border of the territory adjacent to the dominant height, at the points where it crosses by transport routes. 7. The method according to p. 1, characterized in that the coordinates of two or more dominant heights of the natural elevation of the earth's surface relative to the sea level are first determined on the potentially threatened territory, while the above-mentioned actions to detect an infectious agent are primarily carried out on parts of the potentially threatened territories adjacent to selected dominant heights located on a potentially threatened territory. 8. The method according to claim 7, characterized in that the dominant heights are chosen along the tectonic uplift line in the form of an anticlise along the line of successive increase in the dominant heights in the direction of the highest height. 9. The method according to p. 1, characterized in that the diagnosis of the selected samples is carried out using the methods of polymerase chain reaction, the reaction of hemadsorption, the reaction of direct immunofluorescence.

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