RU2615154C1 - Method to increase average and maximum lifespan and fertility of mammals - Google Patents

Abstract

FIELD: biotechnology.

SUBSTANCE: use of boundary water - Avoda preparation - is described as part of the method to increase lifespan and fertility of mammals. The invention can be used to increase survival, the average and maximum lifespan of mammals, including humans, and to improve their fertility, as well as to increase females safety and reduce their puerperium.

EFFECT: improved quality and length of human life, intensified private and industrial farming.

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Description

The invention relates to biochemistry. In particular, the invention relates to the use of border water - the Avoda preparation, in a method for increasing life expectancy and increasing mammalian fertility. The invention can be used to increase the survival rate, average and maximum life expectancy of mammals, including humans, and increase their fertility (fecundity), as well as to increase the safety of females and reduce their postpartum period. The invention can be used in biology, medicine and veterinary medicine and can be used to improve the quality and longevity of people, as well as to intensify private and industrial animal husbandry.

The use of various substances to increase the life or fertility of animals is known in the art. In particular, from RU 2501225 there is known a means for increasing the life span of Drosophila melanogaster containing an active substance taken at a concentration of 20 mg / l, characterized in that ammonium pyrrolidine dithiocarbamate (PDTC) is a low molecular weight thiol compound. From RU 2269357, a method is known for increasing the lifespan of humans and animals and slowing the onset of disease states associated with increasing age, characterized in that an agent that inactivates extracellular blood DNA is introduced into the systemic circulation. For example, the enzyme DNase. From RU2169575 there is known a preparation for slowing down the aging of the body, containing garlic and cinnamon, characterized in that it additionally contains long turmeric, damask blackberry, mirobalan, medicinal ginger, cardamom, cubeb pepper, long pepper, sugar. From RU 2010102484 a method of using royal jelly for increasing fertility is known, which consists in oral administration of royal jelly, characterized in that royal jelly is administered in an amount of 250-300 mg under the tongue 1-3 times a day 30-60 minutes after eating for 15 -25 days 2-3 times a year.

However, in the prior art there are no documents disclosing agents or preparations that would have both the effect of lengthening the life span and the effect of enhancing fertility in animals and, in particular, mammals.

At the present stage of technological development, there is no doubt the need for new available means to extend life and increase fertility in mammals.

The subject of the present invention is a method for increasing the average and maximum life expectancy and fertility of mammals. This problem is solved through the daily use of Avoda for oral administration in a selected dose, calculated on the basis of the weight of the mammal, about 1-2.5 μl per 1 g of weight, while the duration of administration can be from one month to several years. The maximum duration of administration may be unlimited, since the drug does not have any toxicity and is not addictive. As for the amount of Avoda administered, studies have shown that administration of Avoda in an amount less than about 1 μl per 1 g of weight does not have a significant effect on the increase in life expectancy and fertility in mammals. And the introduction of Avoda in an amount greater than 2.5 μl per 1 g of weight does not make sense, since a reliable reliable positive effect, achieved even with this amount, does not have a pronounced improvement with increasing dose.

Avoda is known from publications 1-3 (References).

Publication 1 theoretically substantiates the mechanism of the positive biological effect of border water (Aqua) on a living organism, including humans

Publication 2 discloses the effect of Avoda on serum toxicity and proliferative activity and cell line viability. It is revealed that proliferation and viability during cultivation with the addition of Avoda practically does not change in normal cells, while in cancer cells inhibition of proliferative activity is noted. Morphological study of cells revealed minor changes in normal lines and complete degeneration in 3 of 6 lines of oncogenic cells.

Publication 3 discloses the use of Avoda for the treatment of urogenital diseases, acute respiratory viral infections and neoplasms.

Example 1

Mammals (mice) of the C57Biack / 6 line were divided into 3 groups of 10 animals per group. The control group was kept under the usual conditions for vivarium, i.e. in a separate cage with a trellised lid, into which a drinking bowl is inserted and a standard granular feed is poured. Animals of the two experimental groups were kept in identical conditions with the control group. The difference was only in the following: animals of the 1st experimental group, starting from the age of 6 months, received 25 μl of border water daily (2.5 days a week) orally. And the animals of the 2nd experimental group, starting from the age of 6 months, received 25 μl of border water orally during the entire observation period.

Animals of the control group and experimental groups were weighed monthly (Fig. 1). The average weight of the animals during the experiment in the experimental groups treated with Avoda - “Border Water” was higher than in the control. What speaks of more comfortable living conditions for animals.

Example 2

Observations of animals revealed the increased survival of mice of the experimental groups (Fig. 2). The average life expectancy of animals, which in the control group was 3.0 years, while in the 1st experimental group, where the animals received border water for 2.5 months, it was 3.5, and in the 2nd experimental group , where animals received border water for almost 4.5 years, the average life expectancy of animals increased to 3.8 years (Fig. 3).

Finally, significant differences in the magnitude of maximum life expectancy should be noted. So, if in the control group it was 3 years and 10 months, then in the 2nd experimental group the maximum life expectancy of animals was 4 years and 6 months.

Example 3

As is known, the ability of female C57Black / 6 mice to reproduce begins at their 3-4 months of age and usually lasts up to 1.5 years. In our studies, we observed the appearance of offspring in the late life of mice. So in the control group in females, only once at the age of 2 years and 6 months was a pregnancy recorded that did not end with the appearance of offspring.

At the same time, in females in the experimental groups aged 2 years 4 months to 3 years 8 months, the number of cases where not only there were several pregnant females, but all 100% of the females were pregnant at the same time, was observed in the first experimental group 6 times, and in the second experimental group 7 times.

These data directly indicate a sharp increase in the fertility of mice under the influence of border water, which is directly confirmed by the number of offspring born. So from 20.10.14 to 08.28.15 no mouse was born in the control group, 28 mice were born in the first group, and 77 in the second group (Fig. 4).

This also directly indicates that in elderly animals between the ages of 2 years and 10 months to 3 years and 8 months, when in the control the females were no longer giving birth, in the experimental groups the females retained the reproductive functions.

Thus, the introduction of border water to mice for 2.5 months or for 4 years allowed, in comparison with the control, to increase survival, average and maximum life expectancy, as well as dramatically increase animal fertility, which was expressed in an increase in pregnancy cycles, the number of newborns and the birth of offspring up to almost 4 years of age.

Based on the data obtained, it is possible to state a certain influence of Avoda on the indicators of animal life. The already gradual, but still noticeable increase in age of the average weight of mice in both experimental groups, compared with the control, gave reason to assume a certain positive effect of Avoda on the general physiological state of animals. In the future, this assumption was significantly confirmed both by an increase in pregnancy cycles in the late stages of females, and by an unexpected sharp increase in animal fertility at this age under the influence of Avod, as evidenced by fertility indicators in the 1st experimental group and especially in the 2nd . In this case, one cannot pay attention to a certain increase in the survival rate of females in both experimental groups compared with the safety of females in the control group. The latter, in all probability, can be explained by their faster rehabilitation after the birth of offspring.

Finally, the last argument directly supporting the positive influence of Avoda on changes in the life indicators of mice should be recognized as a pronounced - by more than 31-35% - increase in its average duration, especially noticeable with prolonged use of Avoda, as well as indicators of maximum life expectancy, increasing under the influence of Avod.

Literature

1. Postnov S.E. The role of water in the boundary layer in a living organism. Biophysical model. // Biomedical radio engineering. 2008. - No. 12. - S. 52-56.

2. Postnov S.E., Mezentseva M.V., Podchernyaeva R.Ya., Dagnlybaeva G.A., Surgucheva I.M., Grinkevich O.M., Lopatina O.A., Baklanova O.V. New approaches in biomedical technology based on water of the boundary layer // Biomedical radio engineering. 2009. - No. 1. - S. 3-15.

3. Zuev V.A., Postnov S.E., Podchernyaeva R.Ya., Mezentseva M.V., Scherbenko V.E. Unusual properties of the water of the boundary layer // Bulletin of the Russian Academy of Natural Sciences. 2009. - T. 9. - No. 3. - S. 12-15.

Claims (1)

A method of increasing the average and maximum life expectancy and fertility of mammals, characterized in that Avoda is orally injected into the body of a mammal daily for at least 1 month in a volume of about 1-2.5 μl per 1 g of weight, either in pure form or in the form of drinking water prepared for use 25-45 minutes before administration by injection of Avoda into it.

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