RU2613473C1 - Method for extracted tooth canal visualising - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: extracted tooth is processed and stored in 3% solution of sodium hypochlorite followed by exposure to 9% nitric acid solution for two days. Then - in 100% alcohol for one day. After alcohol exposure, the tooth is exposed in the glycerine solution for two hours.

EFFECT: method allows to increase the degree and duration of teeth transparency preservation, which makes it possible to carry out a visual demonstration of the three-dimensional anatomy of the root canal.

3 dwg

Description

The invention relates to medicine, namely to dentistry, and can be used to visualize the root canal of a removed tooth when studying the anatomy of root canals, assessing the quality of root canal filling, assessing the quality of materials for root canal filling.

A known method of visualizing the root canal of a removed tooth, in which the extracted tooth is stored in a 10% formaldehyde solution, is placed in a 4.2% sodium hypochlorite solution to remove organic tissues, then for 20 minutes in an ultrasonic cleaner, in a 5% HNO ₃ solution for 3 days, while changing the solution every 8 hours, periodically mixing. Wash samples with running water for 4 hours. Then use alcohol solutions: 60% ethyl alcohol - for 8 hours, 80% alcohol - for 4 hours, 96.6% ethyl alcohol - for 2 hours. For 2 hours, placed in xylene and then stored in methyl ester of salicylic acid (methyl salicylate) [Sergio Rosler. Transparent teeth: a great visual aid. Dental Tribune. No. 2, volume 10. - April 2011. - p. 22-23].

The method has disadvantages. The total time to achieve the result is at least 4 days. The implementation of the method is time-consuming: the need to change the HNO ₃ solution every 8 hours, periodically mix it, rinse the samples with water for 4 hours, make 3 types of alcohol concentration, change them every 8-4-2 hours. The need for the constant presence of a researcher. The use of ultrasound requires high laboratory equipment. After achieving transparency during storage in a solution of methyl salicylate, the tooth gradually becomes cloudy [RU 2486601, publ. 06/27/2013. Bull. No. 18, p. 3].

A known method of visualizing the root canal of a removed tooth, including exposing the tooth sequentially in solutions of nitric acid, alcohol and xylene, in which the extracted tooth is placed and stored in a 3% sodium hypochlorite solution, is then exposed in a 9% nitric acid solution for two days, in 100 % alcohol - for one day and in a solution of xylene - for three hours [RU 2373582, publ. November 20, 2009].

The method has disadvantages: teeth subjected to xylene treatment quickly lose transparency in air and gradually become cloudy when stored in solution. In addition, xylene (xylene) is a chemical substance belonging to hazard class 3:

- xylen vapors at high concentrations cause narcotic effects, adversely affect the nervous system, cause irritation of the skin (dermatitis) and the mucous membrane of the eyes;

- bad effect on the kidneys and excretory system as a whole;

- affects the respiratory tract and blood-forming organs;

- is combustible, explosive;

- rooms in which work is carried out with xylene must be provided with supply and exhaust ventilation, and equipment - with local suction.

A known method of visualizing the root canal of a removed tooth, in which the extracted teeth are processed and stored in a 3% sodium hypochlorite solution. To visualize the root canal, the extracted tooth is placed in a 9% solution of nitric acid for two days, and then in 100% alcohol for one day. After exposure to 100% alcohol, the tooth is placed in a methyl salicylate solution for two hours. A transparent tooth is stored in a solution of methyl salicylate [RU 2486601, publ. 06/27/2013. Bull. No. 18]. This method is accepted by the authors as a prototype.

However, teeth enlightened in this way quickly lost their transparency after they were removed from the methyl salicylate solution.

The aim of the proposed method is to improve the quality of enlightenment of the extracted teeth to study the morphology of the root canal system and to evaluate the materials and methods of root canal filling.

This goal is achieved by the fact that in the known method of visualizing the root canal of a extracted tooth, including processing and storing the tooth in a 3% sodium hypochlorite solution, subsequent exposure in a 9% nitric acid solution for two days and in 100% alcohol for one day, according to the invention, after exposure to 100% alcohol, the tooth is exposed in a solution of glycerol for two hours.

Glycerin has three hydrophilic groups that are responsible for its solubility in water and its hygroscopic nature [Pagliaro M., Rossi M. "The Future of Glycerol: New Uses of a Versatile Raw Material". 2008, p. 2].

Glycerin draws water from the environment, used in medical and pharmaceutical preparations, mainly as a means of improving smoothness, providing lubrication, and as a humectant, that is, in the form of a hygroscopic substance, helps control the activity of water in the composition of the main substance [Pagliaro M, Rossi M. "The Future of Glycerol: New Uses of a Versatile Raw Material." 2008, p. 7, 8].

The effect of transparency of the teeth is stored for a long time in a solution of glycerol and is not lost in air. The studies showed that the extracted teeth treated by the proposed method remain transparent as in a solution in air, and after 1 day also retain transparency (Fig. 3), while the degree of transparency visually increases compared to the prototype.

Thus, the use of methyl salicylate causes a loss of tooth transparency in the air.

The advantage of the proposed method over the prototype is illustrated by figures 1-3.

In FIG. 1 - image of transparent teeth with visualized root canals after extracting them from solutions: according to the proposed method (a) and prototype (b), where 1 is the root canals.

In FIG. 2 - image of transparent teeth with visualized root canals 30 minutes after extracting them from solutions: according to the proposed method (a) and prototype (b), where 1 is the root canals.

In FIG. 3 - image of transparent teeth with visualized root canals 24 hours after extracting them from solutions: according to the proposed method (a) and prototype (b), where 1 is the root canals. The method is as follows.

The extracted teeth are treated and stored in a 3% sodium hypochlorite solution. To visualize the root canal, the extracted tooth is placed in a 9% nitric acid solution for two days, then in 100% alcohol for one day. After exposure to 100% alcohol, the tooth is placed in a glycerol solution for two hours. A transparent tooth is stored in a solution of glycerol.

The proposed method processed 50 teeth. All treated teeth remained transparent in air for 1 day.

The proposed method allows to obtain transparent tooth samples using glycerol, a safe substance, due to which the teeth become transparent (the degree of transparency is visually higher than when using methyl salicylate) and retain this property both in solution and for a long time in air, allowing researchers to conduct a visual demonstration three-dimensional anatomy of the root canals, as well as analyze new instrument systems on transparent teeth under the conditions of three-dimensional anatomy of tooth canals.

Claims (1)

A method for visualizing the root canal of a extracted tooth, including processing and storing a tooth in a 3% sodium hypochlorite solution, subsequent exposure in a 9% nitric acid solution for two days and in 100% alcohol for one day, characterized in that after exposure in alcohol, the tooth is exposed in a glycerol solution for two hours.

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