RU2600889C1 - Method for selection of young cattle at growth rate - Google Patents
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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Abstract
Description
Предлагаемое изобретение относится к селекции и генетике крупного рогатого скота и предназначено для раннего отбора животных с целью повышения скорости роста в популяциях скота черно-пестрой породы.The present invention relates to breeding and genetics of cattle and is intended for early selection of animals in order to increase the growth rate in populations of black-motley cattle.
Скорость роста является одним из важнейших экономических показателей, влияющим на выход мяса, формирование конституции, экстерьера и интерьера у сельскохозяйственных животных, и используется в качестве селекционного признака. Выявлено влияние SNP гена лептина на индексы длины и ширины туловища, массы и высоты тела, а также связан с содержанием жировой ткани в сердце, печени, почках, селезенке, легких и мышцах у Luxi породы крупного рогатого скота. Установлено так же, что гены фактора роста Septin-7 (СВС10) и Atrogin-1 влияют на развитие мышечной массы и признаки роста коров. В частности, SNP гена Septin-7 влиял на динамику роста у животных трех японских пород скота и серой швейцарской породы (Tong В. et al., 2015). Длина туловища скота породы Nanyang связана с 4 SNPs гена Atrogin-1. Имеется корреляция SNPs генов бычьего инсулиноподобного фактора роста (IGF-I) и миогенного фактора (MYF5) с показателями роста. Показано, что коровы с генотипом АВ имели большую массу тела в возрасте 90 дней, чем животные, с гомозиготным генотипом ВВ. При этом SNP гена MYF5 оказывал влияние на массу животных в возрасте 12 мес и величину среднесуточного прироста (Chung Е.R., Kim W.T., 2005).The growth rate is one of the most important economic indicators affecting the yield of meat, the formation of the constitution, exterior and interior of farm animals, and is used as a selection trait. The effect of the leptin SNP gene on the indices of body length and width, body weight and height was revealed, and is also associated with the content of adipose tissue in the heart, liver, kidneys, spleen, lungs and muscles in Luxi cattle. It was also established that the genes of the growth factor Septin-7 (CBC10) and Atrogin-1 affect the development of muscle mass and signs of growth of cows. In particular, the Septin-7 SNP gene influenced the growth dynamics in animals of three Japanese cattle and Swiss gray breeds (Tong B. et al., 2015). The body length of the cattle of the Nanyang breed is associated with 4 SNPs of the Atrogin-1 gene. There is a correlation of SNPs of bovine insulin-like growth factor (IGF-I) and myogenic factor (MYF5) genes with growth rates. It was shown that cows with the AB genotype had a larger body mass at the age of 90 days than animals with the homozygous BB genotype. At the same time, the SNP of the MYF5 gene influenced the weight of animals at the age of 12 months and the value of the average daily gain (Chung E.R., Kim W.T., 2005).
Предлагаемый способ основан на использовании однонуклеотидного полиморфизма гена фактора некроза опухолей (Tumor Necrosis Factor, TNF). Однонуклеотидный и другие типы полиморфизма широко применяются как молекулярно-генетический маркер наследственных аномалий, продуктивности, устойчивости к болезням (Zheltikov A.I. et al., 1996). Заявляемый способ отбора крупного рогатого скота черно-пестрой породы по показателям скорости роста включает выделение ДНК из крови с дальнейшим генотипированием коров с помощью полимеразной цепной реакции и отбор телят от гомозиготных А/А и гетерозиготных A/G матерей по гену TNF-α -824A/G.The proposed method is based on the use of single nucleotide polymorphism of the gene for tumor necrosis factor (Tumor Necrosis Factor, TNF). Single nucleotide and other types of polymorphism are widely used as a molecular genetic marker of hereditary abnormalities, productivity, and disease resistance (Zheltikov A.I. et al., 1996). The inventive method for the selection of cattle of black-motley breed in terms of growth rate includes the extraction of DNA from the blood with further genotyping of cows using the polymerase chain reaction and the selection of calves from homozygous A / A and heterozygous A / G mothers for the TNF-α -824A / G.
Пример выполненияExecution example
Для исследований отбирали пробы венозной крови лактирующих коров черно-пестрой породы в возрасте 1-5 лактаций, разводимых в хозяйстве СПК «Кирзинский» в Новосибирской области. Эти животные исследованы по SNPs TNF-α -824A/G и TNFR1 -1703С/Т. Были проанализированы показатели живой массы в различные периоды онтогенеза у 100 телят, полученных от генотипированных коров.Samples of venous blood of lactating cows of black-motley breed at the age of 1-5 lactations, bred at the farm of the SEC "Kirzinsky" in the Novosibirsk region were taken for research. These animals were tested for SNPs TNF-α-824A / G and TNFR1 -1703C / T. The indicators of live weight were analyzed at different periods of ontogenesis in 100 calves obtained from genotyped cows.
Изучение однонуклеотидного полиморфизма проводилось в лаборатории Института цитологии и генетики СО РАН. ДНК из венозной крови выделяли стандартным методом фенольно-протеолитической экстракции. Фрагмент гена TNF-α крупного рогатого скота исследовали с применением метода ПЦР-ПДРФ с использованием прямого праймера 5′-CCGAGAAATGGGACAACCT-3′ и обратного праймера 5′-GCCATGTATCCCCAAAGAAT-3′. Анализ проводили на амплификаторе «Терцик» (ДНК-технологии, Россия), в течение 35 циклов при температуре отжига 60°С. Реакция проходила в ПЦР SE буфере G. Продукт ПЦР оценивали вертикальным электрофорезом в 4% ПААГ, окрашенным бромистым этидием. В продукт амплификации добавляли эндонуклеазу рестрикции EcoICRi, а затем оценивали в 4% ПААГ, окрашенном бромистым этидием. Определение однонуклеотидного полиморфизма TNFR1 -1703С/Т проводилось методом постановки аллель-специфической ПЦР в SE буфере G с использованием праймеров 5′-1872-GGCTGCCAGATCGTGCCTGC-3′-общий, по нижней цепи 5′-1686-TCCGAGCCCCGCCTTCTGT-3′- для дикого типа, по верхней цепи и 5′-1686-TCCGAGCCCCGCCTTCTAC-3′- для мутантного типа. Аллель-специфическую ПЦР проводили в течение 35 циклов при температуре отжига 60°С. Продукт ПЦР оценивали вертикальным электрофорезом в 4% ПААГ, окрашенном бромистым этидием. Для статистической обработки использовался процессор редактора Gnumeric.The study of single nucleotide polymorphism was carried out in the laboratory of the Institute of Cytology and Genetics SB RAS. DNA from venous blood was isolated using a standard phenolic proteolytic extraction method. The bovine TNF-α gene fragment was examined using PCR-RFLP using the 5′-CCGAGAAATGGGACAACCT-3 ′ forward primer and the 5′-GCCATGTATCCCCAAAGAAT-3 ′ reverse primer. The analysis was performed on a Tertsik thermocycler (DNA technology, Russia) for 35 cycles at an annealing temperature of 60 ° С. The reaction was carried out in PCR SE buffer G. The PCR product was evaluated by vertical electrophoresis in 4% SDS page stained with ethidium bromide. EcoICRi restriction endonuclease was added to the amplification product, and then evaluated in 4% SDS page stained with ethidium bromide. The determination of the TNFR1 -1703C / T single nucleotide polymorphism was carried out by the method of setting allele-specific PCR in SE buffer G using primers 5′-1872-GGCTGCCAGATCGTGCCTGC-3′-generic, 5′-1686-TCCGAGCCCCGGCCTTCTGT-3 for dic 3 , on the upper chain and 5′-1686-TCCGAGCCCCGCCTTCTAC-3′- for the mutant type. Allele-specific PCR was performed for 35 cycles at an annealing temperature of 60 ° C. The PCR product was evaluated by vertical electrophoresis in 4% SDS page stained with ethidium bromide. For statistical processing, the processor of the Gnumeric editor was used.
В таблицах 1 и 2 представлены результаты анализа массы тела телят в возрасте 18 мес, полученных от коров с различными генотипами по SNPs TNF-α -824A/G и TNFR1 -1703С/Т.Tables 1 and 2 present the results of body mass analysis of calves at the age of 18 months obtained from cows with different genotypes for SNPs TNF-α-824A / G and TNFR1 -1703C / T.
Установлены значительные различия по живой массе молодняка в зависимости от генотипов матерей по SNP TNF-α -824A/G. Животные, полученные от гомозиготных (А/А) и гетерозиготных (A/G) коров, имели живую массу на 10,9% выше, чем потомство от гомозигот (G/G) (Р<0,05). Не выявлено различий по живой массе телят в зависимости от генотипов матерей по SNP TNFR1 -1703С/Т.Significant differences were found in the live weight of young animals, depending on the mother genotypes according to SNF TNF-α-824A / G. Animals obtained from homozygous (A / A) and heterozygous (A / G) cows had a live weight of 10.9% higher than offspring from homozygous (G / G) (P <0.05). There were no differences in live weight of calves depending on mother genotypes for SNP TNFR1 -1703C / T.
Таким образом, в изученном стаде SNP TNF-α -824A/G связан со скоростью роста молодняка, что позволяет использовать его в качестве маркера при отборе ремонтных телок и племенного молодняка. В дальнейшем селекционную стратегию необходимо корректировать с уточнением уровня продуктивности и условий среды. У животных других пород использование однонуклеотидного полиморфизма TNF-α -824A/G в качестве молекулярно-генетического маркера возможно только после предварительного тестирования популяции животных. При отборе телок следует отдавать предпочтение гомозиготным (А/А) и гетерозиготным (A/G) матерям при прочих равных условиях. Предлагаемый способ позволяет проводить ранний отбор животных с целью повышения скорости роста в популяциях скота черно-пестрой породы.Thus, in the studied herd SNP TNF-α-824A / G is associated with the growth rate of young animals, which allows it to be used as a marker in the selection of repair heifers and breeding young animals. In the future, the breeding strategy must be adjusted to clarify the level of productivity and environmental conditions. In animals of other breeds, the use of the TNF-α-824A / G single nucleotide polymorphism as a molecular genetic marker is possible only after preliminary testing of the animal population. In the selection of heifers, homozygous (A / A) and heterozygous (A / G) mothers should be preferred, ceteris paribus. The proposed method allows for the early selection of animals in order to increase the growth rate in livestock populations of black-motley breed.
Библиографический списокBibliographic list
1. Chung E.R. Association of SNP marker in IGF-I and MYF5 candidate genes with growth traits in Korean cattle / E.R. Chung, W.T. Kim // Asian Austral. J. Anim. Sci. - 2005. - V. 18. - №8. - P. 1061-1065.1. Chung E.R. Association of SNP marker in IGF-I and MYF5 candidate genes with growth traits in Korean cattle / E.R. Chung, W.T. Kim // Asian Austral. J. Anim. Sci. - 2005. - V. 18. - No. 8. - P. 1061-1065.
2. Tong B. Association of the expression levels in the skeletal muscle and a SNP in the CDC 10 gene with grownh-relates traits in Japanese black beef cattle / B. Tong, G.P. Li, S. Sasaki et.al. // Animal genetics. - 2015. - V. 46. - №2. - P. 200-204.2. Tong B. Association of the expression levels in the skeletal muscle and a SNP in the CDC 10 gene with grownh-related traits in Japanese black beef cattle / B. Tong, G.P. Li, S. Sasaki et.al. // Animal genetics. - 2015. - V. 46. - No. 2. - P. 200-204.
3. Yang D. Association of Single SNP and Q-PCR of Leptin gene with grown traits in chinese Luxi cattle / D. Yang // Journal of animal and veterinary advances, 2013. - V. 12. - №6. - P. 791-794.3. Yang D. Association of Single SNP and Q-PCR of Leptin gene with grown traits in chinese Luxi cattle / D. Yang // Journal of animal and veterinary advances, 2013. - V. 12. - No. 6. - P. 791-794.
4. Zheltikov A.I. Immunogenetic structure in a population of Black and White cattle in West Siberia / A.I. Zheltikov, V.G. Marenkov, V.L. Petukhov // XXVth International Conference on Animal Genetics, 1996. - P. 61-62.4. Zheltikov A.I. Immunogenetic structure in a population of Black and White cattle in West Siberia / A.I. Zheltikov, V.G. Marenkov, V.L. Petukhov // XXVth International Conference on Animal Genetics, 1996 .-- P. 61-62.
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| RU2784587C1 (en) * | 2022-09-05 | 2022-11-28 | Федеральное государственное бюджетное учреждение науки Федеральный исследовательский центр "КОМИ научный центр Уральского отделения Российской академии наук" | Method for selecting calves with high live weight gain potential |
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|---|---|---|---|---|
| RU2784587C1 (en) * | 2022-09-05 | 2022-11-28 | Федеральное государственное бюджетное учреждение науки Федеральный исследовательский центр "КОМИ научный центр Уральского отделения Российской академии наук" | Method for selecting calves with high live weight gain potential |
| RU2794793C1 (en) * | 2022-11-04 | 2023-04-25 | Федеральное Государственное бюджетное научное учреждение "Федеральный научный центр биологических систем и агротехнологий российской академии наук" | METHOD FOR SELECTION OF CATTLE INDIVIDUALS WITH HIGH LEVEL OF MILK PRODUCTION, Ca AND Mn BY LPR4 GENE POLYMORPHISM |
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