RU2574023C2 - Bacteriophage-containing room deodorant - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: room deodorant contains a concentrate of selected high-avidity bacteriophages of the species Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pyogenes, Salmonella enterica, Proteus mirabilis, Proteus vulgaris, Klebsiella pneumoniae, Yersinia pseudotuberculosis, Yersinia enterocolitica, Pseudomonas aeruginosa. The concentrate of the above bacteriophages possesses Appelman's lytic activity 10-8 of each bacteriophage. The bacteriophages are grown on a dense medium and purified by ultrafiltration. The above agent contains a perfume compound and isothiozolinone-based preserving agents not reducing bacteriophage activity. Bacteria nutrients are absent.

EFFECT: using the invention provides eliminating unpleasant odours and bacteria of the species Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pyogenes, Salmonella enterica, Echerichia coli, Proteus mirabilis, Proteus vulgaris, Klebsiella pneumoniae, Yersinia pseudotuberculosis, Yersinia enterocolitica, Pseudomonas aeruginosa, as well as prevents bacterial infections caused by the above bacterial species.

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Description

The invention relates to biotechnology, medicine, veterinary medicine, sanitation.

Biological preparations of bacteriophages are filtrates completely lysed by one or another phage of the corresponding broth culture of bacteria. Polyphages are biological products that contain from two or more bacteriophages. As a rule, before mixing, a titer of bacteriophages is established and, depending on the content of viral particles, 1 ml of the phagolysate is mixed. According to some researchers, antagonism between bacteriophages in biologics of the polyphage does not occur (RU 1697421; RU 2410084; RU 1533330). Many studies have noted the complete harmlessness of bacteriophages for the body, administered even in large doses and repeatedly, including the intravenous and intralumbal route of administration. Biological preparations of polyphages used for sanitation of premises should not contain viable bacterial cells. The biopharmaceutical of the polyphage used for these purposes should also not contain aggressive and corrosive substances. The main quality criteria for biopharmaceuticals of polyphages are: the viability of bacteriophages (stability of the phage titer), the breadth of the spectrum of sensitive bacteria. In addition, biopharmaceutical solutions of polyphages should not contain nutrients that may be available to bacteria that are not sensitive to bacteriophages that make up the product. Biological preparations of bacteriophages should not contain endotoxins, which are released during the lysis (destruction) of cells. Also, biopharmaceuticals of polyphages should not contain substances that inhibit or have a negative effect on bacteriophages.

The invention is known "Probiotic drug for veterinary use" (RU 99112455). The invention contains a bacterial mass of bifidobacteria, streptococci and streptococcal bacteriophages. However, for aerosol use, this drug is not acceptable.

The invention is known - a composition for oral administration, containing non-pathogenic microorganisms having the ability to normalize the intestinal microflora (options) (RU 2008131894). The basis of the composition are bacteriophages. However, for aerosol use, this drug is not acceptable.

Known tool - suppositories containing bacteriophages (RU 2000115937). However, for aerosol use, this drug is not acceptable.

A known method of processing bacteriophages of premises for economic purposes (GEU 20071393). However, the disadvantages of this method include the lack of deodorization effect.

The invention is known, an antibacterial selective agent based on bacteriophages (RU 2366436), which may be a prototype. This invention relates to biotechnology, medicine, pharmacy, cosmetology and veterinary medicine and can be used in the manufacture of therapeutic prophylactic and sanitary-hygienic means. The known agent contains bacteriophages that selectively inactivate pathogenic microflora, and components that stimulate the growth of normoflora. Bacteriophages are contained in the agent in an amount of 0.01-0.1 wt.% With a concentration of 10 ^-5 in 1 ml according to Appelman. The disadvantage of the proposed means for aerosol treatment of premises is a narrow range of bacteriophages Staphylococcus spp., Streptococcus spp., Proteus spp., Echerichia spp. and the possible content of endotoxins in connection with the technology of cultivation in liquid media and the lack of control on the endotoxins of these bacteria.

The objective of the invention is the creation of a deodorizing agent for the selective inactivation of a wide range of bacteria of the species Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pyogenes, Salmonella enterica, Proteus mirabilis, Proteus vulgaris, Yersinia pseudotuberculosis in an environment of cultivation, Persudomida pseudoma obtaining high titers of bacteriophages, the absence of endotoxins in the product and nutrients for the reproduction of bacteria, as well as preservatives based on isothiazolinone, which do not reduce the activity of bacteriophages.

As a result of the use of the present invention, it becomes possible to expand the range of deodorizing agents for rooms with highly effective antibacterial action of a selective purpose by combining the action of perfume and selected highly specific bacteriophages Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pyogenes, Salmonella enterica, Proteus mirabilis, Proteusululgaria pereususulgaria vulgaris, Proteus vulgaris, Proteus vulgaris enterocolitica, Pseudomonas aeruginosa.

Aerosol application of the proposed tool provides contact of bacteriophages and sensitive bacteria with subsequent inactivation, improving the quality of human life and preventing the spread of infections of microbial etiology. An example of the composition of the tool is shown in table 1.

The above result is achieved by the fact that the proposed deodorizing agent with selective antibacterial action, based on bacteriophages includes the following.

1. A wide range of selected highly specific bacteriophages grown in solid medium and purified by ultrafiltration with the properties indicated in table 2.

2. The lack of nutrients in the composition eliminates the development of bacteria.

3. The absence of endotoxins in the composition of the product, achieved by the fact that 1 ml of flushing of the phagolysate from a dense medium is diluted more than a million times in the final finished product (product). For example, 1 ml of washout from the dense medium of the bacteriophage Klebsiella pneumoniae with an initial titer of 5 * 10 ¹⁴ , and containing 10 units. endotoxin, diluted in one hundred liters of funds, that is, 100,000 times. The concentration in the composition of the agent at the bacteriophage will be 5 * 10 ⁹ PFU / ml, and the concentration of endotoxin is 0.0001 units. The concentration of the toxin is controlled as part of the spray using kits or liquid chromatography.

4. Preservatives based on isothiozolinone that do not reduce the activity of bacteriophages, but inhibit the growth of bacteria, yeast and molds in concentrations of 0.01-0.1 g / l.

5. Isotonic solution of 0.8% -0.9% sodium chloride, allows you to protect bacteriophages in the composition of the product from such a thing as osmotic shock.

Based on the tests, the bacteriophages of the bacteria Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pyogenes, Salmonella enterica, Proteus mirabilis, Proteus vulgaris, Yersinia pseudotuberculosis, Yersinia enterocolitica, Pseudomonas aeruginosa do not reduce the activity of the deodorizer for 1 year.

When determining the effectiveness of the spray for the premises, the specifics of its application were taken into account. The criterion of effectiveness was considered to be a decrease in the titer of bacteria on the treated surface by 3 or more times. For this purpose, daily cultures of phage-specific bacteria were mixed at a concentration of not less than 10 ⁸ and a sterile surface of 1 m ^{2 was} treated. Then, a flush was carried out with 100 cm ² , treated with a room tool, the exposure was maintained for 24 hours, and a subsequent flush with 100 cm ^{2 of the} surface was performed. The following results were obtained, are shown in table 3.

The deodorizing effect was determined organoleptically with the participation of a group of volunteers, it was found that to achieve this effect, on average, it is necessary to spray 1 ml of the product in a room with a volume of 20 m ³ .

The proposed deodorizing agent for rooms containing selected highly specific bacteriophages has an antibacterial effect and is refreshing due to aromatic fragrances, and the concentration of bacteriophages of the species Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pyogenes, Salmonella enterica pica, Proteusulia pica vulgaris pica, Proteus vulgaris pica, Proteus vulgaris enterica, Proteus vulgaris enterica vulgaris makes 10 ^-8 in 1 ml of means according to Appelman. The complete absence of toxins and the high specificity of bacteriophages make it possible to use this product in any room. Thus, the proposed tool meets the criterion of "novelty."

Claims (1)

A deodorizing agent for rooms containing a bacteriophage concentrate and perfume, characterized in that it contains highly specific selected bacteriophages of the species Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pyogenes, Salmonella enterica, Proteus mirabitofibuda, Proteus vulgaris, Yersiniaocolisseud, Proteus vulgaris pseudophysica possesses lytic activity of each bacteriophage 10-8 according to Appelman, bacteriophages are grown in solid medium and purified by ultrafiltration, also contains preservatives based on isothiosolinone, which do not reduce activity akteriofagov, with pumps for bacterial components are missing.