RU2573450C1 - Method of identifying target genes for diagnostics and therapy of human leucoses - Google Patents

Abstract

FIELD: biotechnology.

SUBSTANCE: essence of the invention is to suppress the activity of the activated oncogene AML-ETO by the method of RNA interference in malignant hematopoietic cells derived from patients with leucosis, using the structure of small hairpin RNA integrated in the lentiviral vector pLSLP-AML-ETO-shRNA, encoded by the nucleotide sequence of double-stranded DNA, which is: 5'-p-gatccgCCTCGAAATCGTACTGAGActtcctgcaa TCTCAGTACGATTTCGAGGtttttg-3' (sense strand), 5'-p-aattcaaaaaCCTCGAAATCGTACT GAGAttgcaggaagTCTCAGTACGATTTCGAGGcg-3' (antisense strand).

EFFECT: inhibition of activity of a particular oncogene by a small hairpin RNA enables to determine the range of changes in the expression of genes responsible for the growth and differentiation of hematopoietic cells, and to detect those of them, the change in the activity of which is directly related to the activation of this oncogene, and to determine the extent to which the impact to such genes - potential targets influences the ability of malignant cells to uncontrolled growth.

2 cl, 7 dwg, 2 tbl, 2 ex

Description

The invention relates to the field of molecular biology and can be used to identify target genes for the diagnosis and treatment of human leukemia.

Leukemia occupies a special place among malignant diseases of humans and animals and is characterized by an increased content of blast cells, which, on the one hand, do not perform the normal functions of mature blood cells, and, on the other hand, due to active proliferation, displace normal hematopoiesis cells.

A significant part of human leukemia is constituted by acute myeloid leukemia (AML). Their occurrence is associated with impaired development of progenitor cells of myeloid, erythroid, megakaryocytic and monocytic cell lines. It is believed that leukemia is of a clonal nature, i.e. malignant blast cells are descendants of one single transformed cell resulting from several, and possibly even one mutations. It is believed that the process of malignant transformation is multi-stage - activation of oncogenes as a result of mutations leads to further activation of a whole cascade of genes and, as a result, to disorganization of strictly coordinated mechanisms responsible for the development of hematopoietic cells and normal blood formation. It is still not known to what extent and which genes are activated as a result of the emergence of already known “key” mutations. All this significantly complicates the diagnosis of leukemia and the choice of effective treatment regimens.

Currently, the main means of treating leukemia are chemotherapy, radiation therapy and bone marrow transplantation. However, the first two methods in themselves are rather painful, far from always effective, and often lead to relapse of the disease, which usually manifests itself in an even more acute form, and the latter is extremely expensive and complicated, which is primarily associated with the search for a donor bone marrow. In addition, bone marrow transplantation also poses a number of health risks to the patient, the main of which include cases of transplant rejection. That is why the search for new effective targets and approaches for the diagnosis and treatment of leukemia is an extremely relevant area of research by scientists around the world.

However, the possibilities of searching for new effective targets are limited by the imperfection of existing methods and their high cost.

The problem is further exacerbated by the fact that it is still not known what other genes are responsible for the growth and differentiation of hematopoietic cells, and to what extent are involved in the process of malignant transformation.

For most types of tumors, only some of the oncogenes involved in carcinogenesis have been identified. This complicates the development and selection of therapeutic approaches, and in particular, therapeutic targets, target genes and their protein products.

In patent RU 2161309 (international publication WO 95/25813) describes the MTS gene, somatic mutations of this gene in the germline, and a method for detecting a predisposition to malignant tumors. The use of identified mutations is also described for the diagnosis of susceptibility to β-different forms of malignant diseases, in particular leukemia, and for the treatment of diseases in which a mutation in the MTS gene occurred, including gene therapy, replacing protein therapy and the use of protein mimetics.

In patent RU 2240125 as a target gene for the treatment of Ph⁺leukemia described oncogen BCR / ABL, and oligonucleotide gene therapy Ph⁺Leukemia by Processing Suspended Ph⁺ cells with antisense oligonucleotides with a length of 16-26 nucleotide units specific for BCR / ABL oncogen mRNA in combination with antisense oligonucleotides for mRNA of other genes that block Ph apoptosis⁺ cells other than the BCR / ABL oncogen.

In patent RU 2286798 A method for identifying chromosomal translocations leading to the development of malignant blood diseases using an oligonucleotide biological microchip by hybridization with an oligonucleotide microchip of a chimeric amplified fluorescently labeled product consisting of gene sequences involved in a particular chromosomal translocation associated with leukemia is described.

Eurasian Patent EA 003637 (international publicationWO 00/05406)new methods for identifying biomolecules of ligands and targets by including random nucleotide sequences in

a framework consisting of a modulator of enzyme activity, transformation of substantially identical cells obtained in this way, and screening of transformed cells to identify cells in which a pre-selected phenotypic trait has been changed.

Eurasian Patent EA 006512 describes methods for determining genes that determine the synthesis of diagnostically important proteins, determining point mutations, deletions, insertions, inversions and translocations in nucleic acids, determining the level of gene expression; determination of homo- and heterozygous states of mutations based on the use of hybridization methods, PCR and its modifications on a microchip.

In the international publication WO 2004/067778 The identification of differentially expressed genes for lymphogranulomatosis, the sequences of these genes and their use as markers for lymphogranulomatosis are described. The gene sequences of the invention are differentially expressed in lymphogranulomatosis.

In the international publication WO 2005/080601 methods of genetic analysis for the classification, diagnosis and prediction of acute myeloid leukemia are described.

In the international publication WO 2010/111712 A method for identifying target RNAs and the use of RNAi for the rational treatment of chemotherapy-resistant leukemia is described. Also presented is a murine model used to determine the effectiveness of shRNA in vivo to reduce the survival of chemotherapy resistant cancer cells.

In the international publication WO 2009/055907 the activity of the non-receptor tyrosine kinase of oncoproteins of the Bcr / Abl fusion gene is described, which are the key factors responsible for the development and progression of Ph + chronic myeloid leukemia and Ph + acute lymphoblastic leukemia. Also described is a peptide inhibitor of Bcr / Abl tyrosine kinase, which induces apoptosis in various transformed cells and inhibits Bcr / Abl kinase and, indirectly, a number of underlying targets (CrkL, STATS, c-Myc).

In the international publication WO 02/42482 functional lentiviral vectors based on the chimeric mouse leukemia virus gene are described and

feline immunodeficiency virus, which are used for gene therapy.

In the international publication WO 2007/050706 describes the use of differential methylation and hybridization to identify new methylation markers and methylation profiles for hematopoietic malignant tumors, leukemia, lymphomas, etc., as well as markers for diagnosis, prognosis and monitoring of treatment.

The use of genome methylation markers to identify new target genes for the diagnosis and treatment of acute myelogenous leukemia is also described in international publication WO 2007/016668 .

Target genes and their expression products, identified on the basis of their expression profile before and after treatment of acute lymphoblastic leukemia, are described in international application WO 03/087315, a study of the gene expression profile for the diagnosis and selection of treatment for leukemia patients is described in WO 03/083140, methods for genotyping cells in acute leukemia ct (llq23) / MLL, and studying the gene expression profile, as well as kits and systems for their implementation, are described in WO 2006/048266, in acute promyelocytic leukemia, in WO 2006/048263.

The identification of a new gene translocation (3p21, 5q33) for myeloid leukemia in humans is described in international publication WO 2007/075933, the identification of the cdc2-dependent kinase gene associated with human leukemia is described in WO 00/12719 ; the identification of the leukocyte-specific Sp 140 gene and its associated protein, and its use in gene therapy for the treatment of malignant diseases, as well as its use as a diagnostic and prognostic marker, are described in WO 98/14569; identification of the MCL-1 gene associated with myeloid leukemia, as well as methods for diagnosis and treatment using the mcl-1 nucleotide and polypeptide sequences, are described in WO 94/29330; an enzymatic RNA molecule that specifically cleaves mRNA encoded by the mdr-1 gene is described in WO 93/23057; the identification of the MTS gene and mutations of this gene, as well as the use in the diagnosis and prognosis, in particular of leukemia, are described in WO 95/25429.

A promising modern approach to studying the functional activity of genes, including activated oncogenes, is RNA interference, based on the suppression of gene expression

at the post-transcriptional level using short siRNA duplexes (small interfering RNAs, small interfering RNAs) 21-23 nucleotides long with prominent 2-3 nucleotides at the 3'-ends. An effective method for introducing interfering RNA into cells is the use of recombinant retro- and lentiviral vectors that direct synthesis of hairpin RNAs (shRNAs) of precursors of small interfering RNAs in transduced cells. siRNAs are formed from shRNAs by the action of Dicer cell protein with ribonuclease activity. Hairpin constructs can be successfully applied to suppress the expression of genes involved in the development of viral infections and some activated oncogenes. It seems possible to apply this approach to suppress the expression of oncogenes detected in acute myeloid leukemia, which is likely to be key factors in the development of AML.

Such approaches and designs of small hairpin RNAs are described, for example, in Chinese Patent Publications CN 102747083,CN 103320444 and in Chinese patent CN 20130925, and in non-patent literature, for example, in BantounasL, Phylactoul L.A., Uney J.B. 2004. RNA interference and the use of small interfering RNA to study gene function in mammalian systems J. Mol. Endocrinol. 33, 545-557, Agrawal N., Dasaradhi P.V., Mohmmed Α., Malhotra P., Bhatnagar R.K., Mukherjee S.K. 2003. RNA interference: biology, mechanism, and applications. Microbiol. Mol. Biol. Rev. 67, 657-685, in an article by Wilhelm A.E., Chumakov SP., Prasolova B.C. 2006 RNA interference: biology and prospects of application in biomedicine and biotechnology. Like biol. 40 (3), 387-403.

However, despite the active identification of new target genes for the diagnosis and treatment of human leukemia and the creation of small hairpin RNA constructs for RNA interference, the problem of finding new effective targets for therapeutic exposure and developing new methods for diagnosing and treating leukemia is still relevant.

The proposed method is based on the suppression of the activity of the activated oncogene AML-ETO by RNA interference in malignant hematopoietic cells obtained from patients with leukemia using the small hairpin RNA construct for insertion into the lentiviral vector at the restriction sites BamHI and EcoRI or Hpal and

Xhol encoded by the nucleotide sequence of double-stranded DNA, which is:

for embedding on BamHI and EcoRI restriction sites

shAEl SLP - semantic

5'-p-

gatccgCCTCGAAATCGTACTGAGActtcctgcaaTCTCAGTACGATTTCGAGGttt ttg-3 '

shAE 1 SLP-antisense 5'-p-

aattcaaaaaCCTCGAAATCGTACTGAGAttgcaggaagTCTCAGTACGATTTCGA GGcg-3 '

or for embedding on the Hpal and Xhol restriction sites

shAE 1 GO-semantic

5'-p-

aacgCCTCGAAATCGTACTGAGActtcctgcaaTCTCAGTACGATTTCGAGGttttt s-3 '

shAE 1 GO-antisense 5'-p-

tcgagaaaaaCCTCGAAATCGTACTGAGAttgcaggaagTCTCAGTACGATTTCGA GGcgtt-3 '

This method allows, by inhibiting the activity of a specific oncogen, to determine the spectrum of changes in the expression of genes responsible for the growth and differentiation of hematopoietic cells, and to find those whose change in activity is directly related to the activation of this oncogen. In addition, this method allows you to determine the extent to which the influence on such potential target genes for the diagnosis and treatment of leukemia affects the ability of malignant cells to uncontrolled growth.

DETAILED DESCRIPTION OF THE INVENTION

The structure of preparations of expression vectors necessary for the construction of preparations of lentiviral particles directing the synthesis of shRNA and suppression of the activated oncogen AMLI-ETOB malignant hematopoietic cells.

The integration of the sequence encoding shRNA in the genome of the host cell can be provided by lentiviral vectors, while long-term suppressing the expression of the target gene. The choice of lentiviral vectors is also determined by the ability of recombinant lentiviruses to infect a wide range of cells, including blood cells. This may allow the use of such vectors to study the effect of suppression of oncogen expression on the restoration of normal differentiation of blood cells, as well as to study genes whose expression will change when the activity of activated AML1-ETO oncogenes is suppressed , which can be promising therapeutic and diagnostic targets in the case of AML.

Such vectors were created on the basis of the pLSLP vector, designed for the transfer and expression of small hairpin RNAs in mammalian cells. The vector allows you to embed the sequence encoding hairpin RNA at the restriction sites BamHI and EcoRI. In this case, the expression of the lentiviral hairpin construct is under the control of the HI polymerase III promoter. In addition, the vector contains a sequence of the puromycin resistance gene, the presence of which allows the selection of cells transduced using this lentiviral construct. The authors of the invention designed the sequences encoding shPHK hairpin structures, the precursors of siPHK:

AML1 -THE-semantic

5'-p-

gatccgCCTCGAAATCGTACTGAGActtcctgcaaTCTCAGTACGATTTCGAGGttt ttg-3 '

AML 1 -ETO-antisense

5'-p-

aattcaaaaaCCTCGAAATCGTACTGAGAttgcaggaagTCTCAGTACGATTTCGA GGcg-3 '

These sequences can be cloned into a lentiviral vector. The basis for such genetic constructs will serve lentiviral vector pLSLP (Figure 1).

The main characteristics of the pLSLP vector are:

The vector is self-inactivating (SIN) due to a deletion in the U3 region of the 3'-terminal LTR.

The vector contains a puromycin resistance gene.

The vector contains the genetic elements of CPPT and WPRE.

Cloning was performed using standard genetic engineering methods. In Figure 2 presents a restriction map of the engineered lentiviral vector pLSLP-AML-ETO-shRNA bearing a hairpin structure specific for the fusion siteAML1-ETO.Similarly, the lentiviral vector pLSLP-SCR-shRNA can be constructed, carrying the sequence encoding shRNA-SCR, not having homology with human and mouse mRNA, used as a negative control.

SCR - semantic

5'-p-

gatccgCAAGTCTCGTATGTAGTGGcttcctgtcaCCACTACATACGAGACTTGtttt tg-3 '

SCR- Antisense 5'-p-

aattcaaaaaCAAGTCTCGTATGTAGTGGtgacaggaagCCACTACATACGAGACT TGcg-3 '

sh-PHK is an expression cassette in the U3 region of 3'dLTR. (The expression cassette is under the control of the RNA polymerase of the Ill-dependent HI promoter.) shRNA expressing the DNA sequence is cloned into the vector at the BamHI and EcoRI restriction sites.

EXAMPLES

EXAMPLE 1

Analysis of suppression of oncogen expression in transplanted hematopoietic cells transduced with a lentiviral vector directing shPHK synthesis, which mediates the suppression of one of the activated oncogenes

To analyze the suppression of the expression of oncogenes in transplanted hematopoietic cells transduced with a lentiviral vector directing shPHK synthesis, which mediates the suppression of activated oncogenes AML1-ETO, whose overexpression is one of the most important factors contributing to the development of leukemia, the RT-PCR method (polymerase valuable reaction, coupled with reverse transcription), a modified method of "nested" RT-PCR and a real-time PCR method (Real-timePCR).

To do this, first defrost laboratory samples of malignant transplantable human cells obtained from a patient with leukemia. For this, ampoules with cell samples are removed from the vessel with liquid nitrogen and placed in a 37 ° C thermostat for 3 minutes, after which the cell suspension is transferred to polypropylene tubes containing 6 ml of RPMI medium and 20% FCS, suspended and centrifuged for 6 min at a speed 800 rpm After that, the supernatant is collected and the pellet is resuspended in 2 ml of RPMI 1640 medium containing 20% fetal serum, 4 mM L-glutamine, streptomycin / penicillin at a concentration of 100 μg / ml and 100 u / ml, respectively, pH 6.8-7 , 0, cultivation is carried out at a temperature of 37 ° C in an atmosphere of 5% CO ₂ . Cell cultivation is carried out in 6 well plates. Three days after thawing, the cells in the well are resuspended, after which 3 ml of fresh RPMI 1640 medium is added, after which they are again resuspended and transferred to a Petri dish with a surface area of 6 cm ² . After another 3-4 days, 5 ml of the cell suspension is transferred to a 10 cm ² Petri dish and 7 ml of fresh RPMI 1640 medium is added. After 4-5 days, the number of cells is counted. To calculate the number of cells using a Neubauer camera. For this, 10 μl of the cell suspension is applied under the coverslip of the chamber. Count the number of cells in four large squares, after which the concentration of cells in the suspension is determined by the formula: C = N * 10000/4, where C is the number of cells in 1 ml of suspension, and N is the number of cells in four squares.

Thus, they build up and prepare for analysis of transplantable human cells that synthesize shPHK constructs (shPHK AML1-ETO, shPHK AML5 '), directing the suppression of AML1-ETO oncogen expression , and one cell sample in which synthesis of the non-specific shSCR construct occurs that does not suppress any of the genes.

Isolation of total RNA from cell cultures in which the synthesis of shRNA - siRNA precursors specific for the AML1-ETO oncogen is carried out and in which the synthesis of the nonspecific shSCR construct occurs is carried out using trisol. For this, about 5 million cells of the cell suspension culture are suspended and transferred to test tubes. Cell tubes are centrifuged for 6 minutes at 1000g. The supernatant is then removed and 1 ml of lxPBS is added, suspended and centrifuged again under the same conditions, after which the supernatant is again removed.

The following reagents are used for isolation:

-Trizol (Invitrogen)

Chloroform (Applied Biosystems)

-Isopropyl alcohol

-75% ethanol (in DEPC water)

-3 Naacetate (pH = 5.5) (Applied Biosystems)

-mQ water

Isolation of total RNA is carried out in the following sequence:

The workplace is treated with RNase inhibitors. Trisol is added to the cells (the required amount is calculated based on the ratio of 1 ml of trizol per 5-10 million cells). Cells are incubated in Trisol for 5 minutes at room temperature. The lysate is transferred to new tubes and chloroform is added at the rate of 0.2 ml per 1 ml of trizole, the tube is tightly closed and shaken by hand for 15 seconds, after which the mixture is incubated for 2-3 minutes at room temperature. Next, the samples are centrifuged for 15 minutes at 12000g in a centrifuge with cooling (4 ° C). As a result of centrifugation, the mixture is separated into a lower red chloroform phenolic phase, a white interphase, and a colorless aqueous phase. RNA is in the upper aqueous phase. The aqueous phase is transferred to a clean tube. Isopropyl alcohol is added at a rate of 0.5 ml per 1 ml of trizole and incubated for 10 minutes at room temperature. Then the samples are centrifuged for 10 minutes at 12000g in a centrifuge with cooling (4 ° C). A light gel-like precipitate is visible at the bottom of the tube. Wash the precipitate with 1 ml of 75% ice ethanol, breaking the precipitate on a vortex. After washing, the samples are centrifuged for 10 minutes at 12000g in a centrifuge with cooling (4 ° C). Washing is carried out twice. After that, the alcohol is removed and the precipitate is dried in air. The precipitate was dissolved in 50 μl mQ (based on 1 μg / μl).

The amount and purity of the isolated RNA is determined using a NanoDrop spectrophotometer, determining the absorbance at 230, 260, 280 nm. The amount of RNA is estimated by the value of A260, because 1 A260 = 40 μg RNA / ml. The purity of the sample is estimated by the ratio of A260 / A280 (should be in the range of 1.8 - 2.0), the degree of contamination with low molecular weight organic compounds - by A260 / A230 (in the range of 1.5 - 2.0). The quality of the isolated RNA is determined by electrophoresis in 1% agarose gel. The presence of two bands corresponding to 28S and 18S ribosomal RNAs, as well as the absence of non-specific bands and a pronounced "Schmer" are assessed.

Next, sodium acetate and ethyl alcohol are added to the RNA solution (1/10 and 2.5 volumes of the RNA solution, respectively). The mixture was vortexed and then incubated for 30 minutes at 20 ° C. The mixture is then centrifuged for 15 min at 10 ° C, 12000 g. The supernatant is removed, 1 ml of chilled 75% ethanol is added to the precipitate. The resulting mixture was vortexed and then centrifuged for 15 min at 10 ° C, 12000 g. The supernatant is removed, the precipitate is dried in air for 10-15 minutes. MQ water is added to the dry cake. The volume of water is calculated based on the initial amount of RNA (μg) and the desired final concentration. In further work, RNA solutions with a concentration of 1 μg / μl are used.

RT-PCR is reverse transcription coupled to a polymerase chain reaction.

For the synthesis of cDNA used total RNA cells (obtained as described above). The isolated RNA is reprecipitated and dissolved in water to a concentration of 1 μg / ml. Mix 1 μl of dissolved RNA, 1 μl of poly-T primers (5 pmol / μl), 4 μl of water treated with DEPC and incubated

the mixture in a water bath at 70 ° C for 5 minutes. Next, cool the mixture on ice for 10 minutes. After a single centrifugation, 4 μl of a five-fold reverse transcriptase buffer (Promega), 2 μl of a dNTP mixture (10 mM, Promega), 1 μl of Rnasine (Promega), 1 μl of MLV reverse transcriptase (Promega) and 6 μl of water treated with DEPC. Vortex is stirred and incubated in a water bath at 42 ° C for 1 hour. Next, the samples are cooled, brought with water mQ, to a final volume of 50 μl. Samples of cDNA stored at -20 ° C.

The analysis of the obtained cDNA by the modified “nested” RT-PCR method is used to identify the total AML-ETO oncogene cDNA obtained from Kasumi-1 cells using the modified nested PCR method with two pairs of primers specific to this gene (“external” for 1- 1st stage, and "internal" - for the 2nd stage). Both stages of nested PCR.

Amplification was carried out under the following conditions, 1 cycle: denaturation 94 ° C for 30 seconds, annealing 30 seconds, synthesis 72 ° C, 1 minute. The annealing temperature was determined for each pair of primers.

To determine the level of gene mRNA expressionAMLl-ETOon the first and second stages, respectively, the optimal number of cycles is 13 and 15 cycles.

Characteristics of the primers used for level analysis: expression of genes of interest are shown in Table 1:

It was shown that in cells of the Kasumi-1 line transduced with lentiviral vectors directing the synthesis of shiRNA precursors of siRNA specific for an activated oncogenAML1-ETOsuppression of the expression of this oncogen, which is key in the malignant degeneration of the hematopoietic tissue and responsible for the differentiation and to some extent the growth of myeloid cells, is suppressed. In Figure 3 Electrophoregram illustrates a significant decrease in the amount of mRNAAML1-ETOin Kasumi-1 cells, in comparison with control cells transduced with lentiviral vectors directing the synthesis of nonspecific shPHK SCR genes.

On the histogram (Figure 4) presents the average values obtained from three independent experiments.

The synthesis of hairpin constructions shPHK-AMLl-ETO or shPHK-AML5 'leads to a decrease in the expression level of AML1-ETO by 3 and 5 times, respectively, compared with the control. As control, Kasumi-1 cells transduced with a lentiviral vector directing the synthesis of shRNA SCR, which has no homology with human and mouse mRNA, were used.

EXAMPLE 2

Analysis of the expression of target genes whose activity is controlled by activated oncogenes

Functional effect exerted by suppressing the expression of activated oncogen AML1-ETO. As an evaluation criterion, the growth rate of Kasumi-1 cells was selected. The growth rate of cells transduced with a lentiviral vector directing specific hairpin constructs specific for the oncogene under study was compared with a control sample of cells transduced with a lentiviral vector directing the synthesis of the non-specific shRNA SCR.

The original Kasumi-1 cells transduced with shPHK SCR, shPHK AML5 'or shPHK-AMLl-ETO are seeded in 96 well plates at concentrations of 4000, 2000, 1000, .500, 250 and 125 cells per well in 100 μl of medium. The experiment is carried out in three repetitions. Cells are grown on

standard RPMI 1640 medium containing 20% fetal serum, 4 mM L-glutamine, streptomycin / penicillin at a concentration of 100 μg / ml and 100 u / ml, respectively, pH 6.8-7.0 at 37 ° C in atmosphere 5 % CO ₂ . Within 12 days after planting, every 2 days, the cells in the wells are resuspended and 5 μl are taken to calculate the number of cells in each well. For 12 days, the amount of medium in each well is monitored at a level of 100 μl.

In Figure 5 a chart illustrating the change in the number of cells in the wells of a 96 well plate over 10 days at an initial planting density of 4000 cells per well. The presented results were obtained from three independent experiments (p <0.05).

It can be seen that the proliferation rate of Kasumi-1 cells in which the expression of endogenous AML1-ETO is suppressed with shPHK-AMLl-ETO is significantly lower than in the original Kasumi-1 cells or with cells in which shPHK-SCR synthesis occurs specific for any of the human or mouse mRNA.

In Figure 6 a histogram is presented illustrating the change in the number of cells in the wells on the tenth day after planting, depending on the density of planting. The presented results were obtained from three independent experiments (p <0.05).

It can be seen that when 4000 cells per well of Kasumi-1 cells, in which AML1-ETO expression is suppressed, are planted, their number is 1.7 times lower than cells in which the level of oncogen expression was not changed. With a planting density of 2000 pcs per well and 1000 pcs per well, these ratios were 4.5 and 14 times. It was shown that at a cell density of Kasumi-1 cells with suppressed AML1-ETO expression below 1000 pcs per well, their growth does not occur at all, while cells with an unchanged oncogen expression level can grow at a relatively rare cell density of 500 pcs. per well and even 250 pcs per well.

These results indicate that the suppression of AML1-ETO oncogen expression in Kasumi-1 cells leads to a significant decrease in their proliferation rate, and also significantly reduces their ability to produce clones and grow at low planting densities relative to the original Kasumi-1 cells with an unchanged expression level oncogene AML1-ETO.

To search for target genes controlled by the activated oncogen AML1-ETO, which may be potential target genes in the development of new approaches for the diagnosis and treatment of AML, the deep sequencing method based on the Illumina platform was used. For this, mRNA is isolated from cells transduced with lentiviral vectors directing shRNA synthesis and cDNA strand synthesis as described above. The analysis of the obtained cDNA samples is carried out using the method of deep sequencing based on the Illumina platform. For each gene, one of the most represented transcripts was selected. To sort the genes by the level of differential expression, the standard deviation of normalized expression was used. Normalization was carried out so that the sum of hits for each sample was equal to this sum in the control sample (cDNA from cells transduced with a lentiviral vector directing the synthesis of shRNA SCR). Genes were sorted by level of differential expression.

In Figure 7 (A, B, C) are examples of histograms of pairwise comparisons of some of the most differentially expressed genes, the expression of which changes as a result of the suppression of the activated oncogen AML1-ETO.

In table 2 A list of all genes whose expression changes when the activated oncogen AML1-ETO is suppressed in the Kasumi-1 cell line is presented. The table shows 5 columns, the first of which contains the identification number of the gene, the second column shows the short name of the gene, the third column shows the number of "reads" for this gene in cells transduced by the lentiviral vector directing the synthesis of shRNA SCR, in the fourth column the number " readings "for a given gene in cells transduced with a lentiviral vector that directs the synthesis of shRNA AML1-ETO, the fifth column shows the ratio of the number of" reads "from the third column to the number of" reads "from the fourth column for yes gene. This ratio illustrates the decrease in the level of expression of the detected gene relative to the control (cells transduced with the lentiviral vector directing the synthesis of shRNA SCR) while suppressing the activated oncogenAML1-ETO.

table 2 ID gene shSCR shAE shSCR / shAE NM 144708 Ankar 28 one 28.0 NM 182689 Efna4 28 one 28.0 NM 153631 Hoxa3 27 one 27.0 NM 004981 KCNJ4 21 one 21.0 NM 000606 C8g 19 one 19.0 NM 001171136 1 ZBTB12 eighteen one 18.0 NM 004943 DMWD 33 16.5 NR 039856 MIR4707 16 one 16,0 NM 003890 FCGBP fifteen one 15.0 NM 025211 Gkap1 fifteen one 15.0 NM 021135 RPS6KA2 fifteen one 15.0 NM 005775 SP6 fifteen one 15.0 NM 001166339 SPDYE5 13 one 13.0 NM 001023561 ZNF774 13 one 13.0 NM 001101426 ISPD 25 12.5 NM 001003674 C18orf1 12 one 12.0 NM 016210 C3orf18 12 one 12.0 NM 033510 DISP2 24 12.0 NR 026771 DKFZP434L187 12 one 12.0 NM 001167857 PPP1R12B 12 one 12.0 NM 130795 Rgs3 24 2 12.0 NM 001636 SLC26A1 24 2 12.0 NM 017575 SMPD3 12 one 12.0 NM 033394 Tap1 24 2 12.0 NM 006297 Yaf2 36 3 12.0 NM 001122962 Sirpb2 22 2 11.0 NM 198216 SNTA1 43 four 10.8 NM 001975 EN02 10 one 10.0 NR 027051 FLJ39582 10 one 10.0 NR 004390 SNORD110 10 one 10.0 NR 000021 SNORD5 10 one 10.0 NM 032786 ZDHHC1 10 one 10.0 NM 001010880 ZNF782 31 3 10.3 NM 001195605 ZNRD1 10 one 10.0 NM 005683 Gpr55 thirty 3 10.0 NM 021184 4 C6orf47 19 2 9.5 NM 001142966 GREB1L 19 2 9.5 NM 198850 PHLDB3 19 2 9.5 NR 037918 PRH1-PRR4 19 2 9.5 NM 001161440 PTPRH 19 2 9.5 NM 017836 SLC44A2 19 2 9.5 NM 138448 ACYP2 28 3 9.3 NM 152240 ZMAT5 46 5 9.2 NM 033031 CCNB3 63 7 9.0 NR 024009 CN5H6.4 9 one 9.0 NM 000106 CYP2D6 eighteen 2 9.0 NM 207305 Foxd4 eighteen 2 9.0 NM 001004056 GRK4 9 one 9.0 NM 172193 KLHDC1 9 one 9.0 NM 203397 MBLAC1 9 one 9.0 NR 039901 MIR4746 9 one 9.0

NR 030368 MIR638 9 one 9.0 NM 024302 MMP28 9 one 9.0 NM 001204295 Muc1 9 one 9.0 NM 020904 PLEKHA4 9 one 9.0 NM 020780 PTCHD2 eighteen 2 9.0 NM 000355 TCTEX1D4 9 one 9.0 NM 003969 UBE2Q2P2 9 one 9.0 NM 001001974 PLEKHA1 34 four 8.5 NR 037428 MIR3655 25 3 8.3 NM 001033082 MYCL1 25 3 8.3 NM 014686 KIAA0355 42 5 8.4 NM 022842 CDCP1 16 2 8.0 NM 032727 INA 33 four 8.3 NR 031576 MIR762 16 2 8.0 NM_007162 TFPI 16 2 8.0 NM 024807 TRIM16L 16 2 8.0 NM 001199161 USP6 33 four 8.3 NM 017715 ZNF319 66 8 8.3 NM 145271 ZNF701 16 2 8.0 NM 024906 SCD5 24 3 8.0 NM 001197129 SYS1-DBNDD2 24 3 8.0 NM 000407 GP1BB 31 four 7.8 NM 173201 ATP2A1 fifteen 2 7.5 NR 027129 C21orf67 fifteen 2 7.5 NM 001098808 C9orf129 thirty four 7.5 NM 001242369 C9orf7 fifteen 2 7.5 NM 015557 Chd5 fifteen 2 7.5 NM 001166346 MDFIC 37 5 7.4 NR 033851 MGC39372 fifteen 2 7.5 NM 020201 NT5M thirty four 7.5 NM 022463 Nxn fifteen 2 7.5 NM 006418 OLFM4 fifteen 2 7.5 NM 017439 Pion fifteen 2 7.5 NM 002702 POU6F1 60 8 7.5 NM 153336 Pstk 22 3 7.3 NM 134428 RFX3 fifteen 2 7.5 NM 014496 RPS6KA6 fifteen 2 7.5 NR 003010 SCARNA12 fifteen 2 7.5 NR 002588 SNORA51 fifteen 2 7.5 NM 016639 TNFSF14 fifteen 2 7.5 NM 001173513 Tymp 22 3 7.3 NM 001102664 EPN2 81 eleven 7.4 NM 033120 NKD2 72 10 7.2 NM 007122 USP18 36 5 7.2 NM 001141936 C4orf48 28 four 7.0 NM 001040113 MYH11 49 7 7.0 NR 028330 C15orf37 21 3 7.0 NM 001039508 Sirpg 21 3 7.0 NM 001080523 ARRDC5 13 2 6.5 NM 152792 ASPRV1 13 2 6.5 NM 001012279 C6orf174 13 2 6.5 NM 017525 CDC42BPG 13 2 6.5 NR 003529 CDKN2B-AS1 13 2 6.5 NM 033641 COL4A6 13 2 6.5

NM 001144962 1 NFKBIL1 13 2 6.5 NM 001017989 OPA3 13 2 6.5 NM 194248 OTOF 13 2 6.5 NM 178518 TMEM107 27 four 6.8 NM 138396 MARCH9 46 7 6.6 NR 027861 ZNF664-FAM101A 33 5 6.6 NR 038 190 3 Ager 19 3 6.3 NM 001145951 TIPARP-AS1 19 3 6.3 NM 001040697 UGT3A2 19 3 6.3 NM 022553 2 VSIG10L 19 3 6.3 NM 014322 OPN3 25 four 6.3 NM 001775 Cd38 82 13 6.3 NM 133463 AMZ1 31 5 6.2 NM 002542 Ogg1 31 5 6.2 NM 181843 NUDT8 one hundred 16 6.3 NM 001164688 RD3 37 6 6.2 NM 207340 ZEB1-AS1 37 6 6.2 NM 016466 ANKRD39 43 7 6.1 NM 004043 1 ASMT 12 2 6.0 NM 005172 ATOH1 12 2 6.0 NM 145178 ATOH7 12 2 6.0 NM 001008223 C1QL4 12 2 6.0 NM 007337 DLEC1 12 2 6.0 NR 002834 DUSP5P 12 2 6.0 NM 023933 FAM173A 24 four 6.0 NM 182759 FAM19A3 12 2 6.0 NM 005803 2 FLOT1 thirty 5 6.0 NM 005309 GPT 24 four 6.0 NM 014234 5 HSD17B8 eighteen 3 6.0 NM 212551 LYSMD1 24 four 6.0 NM 020932 MAGEE1 12 2 6.0 NM 018670 MESP1 12 2 6.0 NM 000253 MTTP 12 2 6.0 NM 024927 PLEKHH3 12 2 6.0 NM 080923 PTPRC 36 6 6.0 NM 032709 Pyroxd2 12 2 6.0 NM 000334 SCN4A 12 2 6.0 NM 006080 SEMA3A 12 2 6.0 NM 003975 SH2D2A eighteen 3 6.0 NM 138356 SHF 12 2 6.0 NM 139157 ST7-AS2 12 2 6.0 NM 003807 TNNI3 12 2 6.0 NM 001142646 Tpsb2 eighteen 3 6.0 NM 001080461 UPP1 12 2 6.0 NM 001128833 ZBTB42 24 four 6.0 NM 021915 ZNF703 12 2 6.0 NM 198540 B3GNT8 106 eighteen 5.9 NM 000054 AVPR2 34 6 5.7 NM 003587 1 DHX16 34 6 5.7 NM 032090 PCDHGA10 34 6 5.7 NM 001172668 ZNF688 34 6 5.7 NR 026811 1 AGSK1 57 10 5.7 NR 026680 MGC57346 28 5 5,6 NM 133475 ANKRD24 22 four 5.5

NM 001855 COL15A1 22 four 5.5 NM 145315 LACE1 22 four 5.5 NR 037421 MIR3648 90 16 5,6 NM 020979 SH2B2 22 four 5.5 NM 032296 Flywch1 94 17 5.5 NM 001887 CRYBB1 16 3 5.3 NM 004409 DMPK 33 6 5.5 NR 033968 GUSBP9 16 3 5.3 NR 036751 HSP90AA6P 16 3 5.3 NM 032452 JPH4 16 3 5.3 NR 028135 KCNMB3 16 3 5.3 NR 039802 MIR4658 16 3 5.3 NM 001083 PDE5A 16 3 5.3 NM 178314 RILPL1 16 3 5.3 NM 000934 SERPINF2 33 6 5.5 NM 024676 SH3D21 33 6 5.5 NM 017844 ANKMY1 60 eleven 5.5 NM 032370 ZNF469 120 22 5.5 NM 001134876 C14orf80 130 24 5,4 NR 002936 TOM1L2 70 13 5,4 NM 181598 ATL1 27 5 5,4 NM 001083537 FAM86B1 27 5 5,4 NM 007085 FSTL1 27 5 5,4 NM 002183 1 IL3RA 27 5 5,4 NM 001105199 TMEM180 54 10 5,4 NM 000099 Cst3 183 34 5,4 NM 080387 CLEC4D 37 7 5.3 NR 024071 PLCD1 37 7 5.3 NM 003794 Sobp 37 7 5.3 NM 032731 TXNRD3 75 fourteen 5,4 NM 001134870 2 KIAA1949 121 23 5.3 NM 002910 RENBP 142 27 5.3 NR 026711 1 ASMTL-AS1 21 four 5.3 NM 130851 BMP4 21 four 5.3 NR 026906 1 C17orf69 10 2 5,0 NM 001374 DNASE1L2 10 2 5,0 NM 017820 EXD3 10 2 5,0 NM 013445 Gad1 21 four 5.3 NM 003516 HIST2H2AA3 10 2 5,0 NM 001242525 2 HLA-DPA1 10 2 5,0 NM 002192 INHBA 10 2 5,0 NM 178863 KCTD13 52 10 5.2 NM 001199659 Letm2 21 four 5.3 NM 002315 LM01 10 2 5,0 NM 001161573 MAFF 21 four 5.3 NM 130385 MRVI1 10 2 5,0 NR 033343 Psca 10 2 5,0 NM 001024938 SLC2A14 10 2 5,0 NM 001082968 TOMM20L 10 2 5,0 NM 001009958 ZNF658B 21 four 5.3 NM 001159293 ZNF75A 10 2 5,0 NM 024876 ADCK4 93 eighteen 5.2 NM 024785 Fam124b 46 9 5.1 NM 032777 Gpr124 108 21 5.1

NM 001098531 RAPGEF3 36 7 5.1 NM 031232 NECAB3 61 12 5.1 NM 130759 GIMAP1 25 5 5,0 NM 018013 Sorbs3 25 5 5,0 NM 080678 UBE2Q2P1 25 5 5,0 NM 005773 ZNF296 51 10 5.1 NM 001010877 3 ZNF321P 66 13 5.1 NM 021630 PDLIM2 96 19 5.1 NM 001136528 SERPINE2 55 eleven 5,0 NM 152783 D2HGDH 115 23 5,0 NM 007053 CD160 fifteen 3 5,0 NM 145019 FAM124A fifteen 3 5,0 NM 024642 Galnt12 fifteen 3 5,0 NM 145910 NEK11 fifteen 3 5,0 NM 016223 Pacsin3 75 fifteen 5,0 NM 001242319 PNMA6D fifteen 3 5,0 NM 030907 RSG1 thirty 6 5,0 NM 001161331 ST20 fifteen 3 5,0 NM 024786 ZDHHC24 148 thirty 4.9 NM 021047 ZNF256 49 10 4.9 NM 020807 ZNF323 49 10 4.9 NM 001170820 IFITM10 34 7 4.9 NM 014055 IFT81 34 7 4.9 NM 024681 KCTD17 34 7 4.9 NM 145232 CTU1 54 eleven 4.9 NM 014694 ADAMTSL2 19 four 4.8 NM 181506 LRRC70 19 four 4.8 NR 026808 NCRNA00321 39 8 4.9 NM 000290 PGAM2 19 four 4.8 NM 003709 KLF7 43 9 4.8 NM 001145784 MEF2BNB 67 fourteen 4.8 NM 014787 DNAJC6 24 5 4.8 NM 139245 PPM1L 24 5 4.8 NM 004159 5 PSMB8 48 10 4.8 NM 001098612 SIGLEC14 24 5 4.8 NM 006470 TRIM22 48 10 4.8 NR 026575 Gk3p 28 6 4.7 NM 005994 TBX3 61 13 4.7 NM 001964 EGR1 33 7 4.7 NM 001101391 Ling03 66 fourteen 4.7 NM 001143821 PLEKHA5 33 7 4.7 NM 182907 PRDM1 33 7 4.7 NM 002904 RDBP 33 7 4.7 NM 020740 ANKFY1 37 8 4.6 NM 001185101 Cd22 42 9 4.7 NM 012446 Ssh1 46 10 4.6 NM 004163 RAB27B 199 43 4.6 NM 001242416 Wdr25 51 eleven 4.6 NM 004029 IRF7 96 21 4.6 NM 001010887 ACER2 22 5 4.4 NM 138430 ADPRHL1 27 6 4,5 NM 001190724 4 ATAT1 9 2 4,5 NM 001141972 ATPBD4 22 5 4.4 NM 174896 C1orf162 9 2 4,5

NR 026784 C6orf164 9 2 4,5 NM012298 Can2 9 2 4,5 NM 000732 Cd3d 13 3 4.3 NM 139158 CDK15 13 3 4.3 NM 153221 Cilp2 9 2 4,5 NM 206833 CTXN1 9 2 4,5 NM 013974 6 Ddah2 eighteen four 4,5 NR 040063 DKFZP564C196 9 2 4,5 NR 026855 DKFZP686I15217 eighteen four 4,5 NM 018163 DNAJC17 13 3 4.3 NM 032281 ELAVL3 9 2 4,5 NM 199280 FAM179A 22 5 4.4 NM 001164379 FAM180B 9 2 4,5 NM 001099338 Fam22a 31 7 4.4 NM 017556 Fblim1 9 2 4,5 NM 145032 FBXL13 13 3 4.3 NR 024101 FLJ35776 9 2 4,5 NM 148899 Foxp2 13 3 4.3 NM 181703 Gja5 9 2 4,5 NM 033177 GPANK1 45 10 4,5 NM 006149 LGALS4 9 2 4,5 NM 001015002 LLGL2 9 2 4,5 NM 005572 LMNA 45 10 4,5 NM 023948 MOSPD3 22 5 4.4 NM 004540 NCAM2 9 2 4,5 NM 201539 NDRG2 9 2 4,5 NM 005386 Nnat 9 2 4,5 NM 006172 NPPA 9 2 4,5 NM 018930 PCDHB10 9 2 4,5 NM 001200053 PMEL 9 2 4,5 NM 014906 PPM1E 9 2 4,5 NM 006663 PPP1R13L 9 2 4,5 NM 004283 RAB3D 22 5 4.4 NM 173587 RCOR2 9 2 4,5 NM 001036 Ryr3 9 2 4,5 NM 001204204 SEC14L2 eighteen four 4,5 NM 004155 SERPINB9 36 8 4,5 NM 003041 SLC7A10 9 2 4,5 NR 004380 SNORD32A 9 2 4,5 NM 201441 Tex22 9 2 4,5 NR 037646 TNFRSF12A eighteen four 4,5 NM 001024843 Tob2p1 58 13 4,5 NM 021138 TRAF3IP2-AS1 22 5 4.4 NM 133462 Ttc16 9 2 4,5 NM 001039783 TYR03P 9 2 4,5 NM 198129 LAMA3 159 36 4.4 NM 001171133 Fam3a 132 thirty 4.4 NM 005072 SLC12A4 57 13 4.4 NM 015177 Dtx4 52 12 4.3 NM 144596 TTYH3 135 31 4.4 NM 001013693 LDLRAD2 178 41 4.3 NM 015916 Calhm2 117 27 4.3 NM 001002837 INPP5J 34 8 4.3 NM 020418 PCBP4 34 8 4.3

NM 016044 FAHD2A 64 fifteen 4.3 NM 004753 DHRS3 94 22 4.3 NM 006820 IFI44L thirty 7 4.3 NM 001135704 ACBD4 25 6 4.2 NR 002710 ALOX12P2 25 6 4.2 NM 014417 Bbc3 25 6 4.2 NM 001178135 HDHD1 25 6 4.2 NM 002207 ITGA9 51 12 4.3 NM 001100425 KIAA0895 51 12 4.3 NR 030407 MIR671 51 12 4.3 NM 020229 PRDM11 25 6 4.2 NM 001199577 GIMAP1-GIMAP5 123 29th 4.2 NM 012458 TIMM8A 160 38 4.2 NM 018132 Cenpq 88 21 4.2 NM 006889 Cd86 21 5 4.2 NM 017803 DUS2L 63 fifteen 4.2 NM 138690 GRIN3B 21 5 4.2 NM 024042 METRN 21 5 4.2 NM 001195628 MLLT10 42 10 4.2 NR 003109 TRO 21 5 4.2 NM 005685 GTF2IRD1 163 39 4.2 NM 006332 IFI30 79 19 4.2 NM 012225 NUBP2 196 47 4.2 NM 021996 GBGT1 58 fourteen 4.1 NM 001135005 DNAJB5 37 9 4.1 NM 021641 ADAM 12 16 four 4.0 NR 027833 APOL3 16 four 4.0 NM 198545 C1orf187 16 four 4.0 NM 014143 CD274 16 four 4.0 NM 001111319 CLDN22 16 four 4.0 NM 152536 Fgd5 16 four 4.0 NR 038275 FLJ31813 16 four 4.0 NM 001517 GTF2H4 16 four 4.0 NR 027386 Gusbp3 16 four 4.0 NM 006896 Hoxa7 16 four 4.0 NM 005931 3 MICB 16 four 4.0 NM 007243 4 Nrm 16 four 4.0 NM 001101401 SBK2 16 four 4.0 NM 001037 SCN1B 16 four 4.0 NM 004858 SLC6A6 49 12 4.1 NM 001199760 ST5 16 four 4.0 NM 198517 TBC1D19 16 four 4.0 NM 015023 Wnk2 49 12 4.1 NM .014699 ZNF653 16 four 4.0 NM 007155 ZSCAN12P1 16 four 4.0 NM 002528 NTHL1 61 fifteen 4.1 NM 199285 PRR19 61 fifteen 4.1 NM 002535 Oas2 45 eleven 4.1 NM 003820 TNIK 73 eighteen 4.1 NM 053050 MRPL53 159 39 4.1 NM 012420 IFIT5 28 7 4.0 NM 018689 KIAA1199 28 7 4.0 NM 001142299 SRCRB4D 28 7 4.0 NM 033093 TRIM71 28 7 4.0

NM 001190810 1 AGAP9 40 10 4.0 NM 004267 CHST2 40 10 4.0 NR 024034 KIAA0664L3 81 twenty 4.1 NM 001204872 NPEPL1 202 fifty 4.0 NM 001017530 PRR5 40 10 4.0 NM 001128635 1 RIMBP3B 40 10 4.0 NM 145159 Jag2 52 13 4.0 NM 018384 GIMAP5 117 29th 4.0 NM 006602 TCN2 64 16 4.0 NM 199165 ADSSL1 12 3 4.0 NM 005582 CD 180 24 6 4.0 NM 206918 DEGS2 12 3 4.0 NM_ 174899 FBX036 12 3 4.0 NM 005897 IPP 24 6 4.0 NR 024061 LOH12CR2 12 3 4.0 NM 002356 MARCKS 24 6 4.0 NR 024322 MSL3P1 36 9 4.0 NM 006212 PFKFB2 84 21 4.0 NM 002632 PGF 12 3 4.0 NM 032882 PNMA6A 12 3 4.0 NM 002800 2 PSMB9 12 3 4.0 NM 144770 RBM11 12 3 4.0 NM 017452 STK19 12 3 4.0 NM 172209 4 TAPBPL 60 fifteen 4.0 NM 006858 TMEM102 108 27 4.0 NM 001145635 TRIM47 12 3 4.0 NM 003443 ZBTB22 24 6 4.0 NM 001170640 CDK20 43 eleven 3.9 NM 007220 CA5B 75 19 3.9 NM 032421 CLIP2 75 19 3.9 NM 032836 Fiz1 75 19 3.9 NM 001146175 ZNF462 31 8 3.9 NM 001042601 Ttc15 177 45 3.9 NM 001014979 C16orf93 51 13 3.9 NM 152320 ZNF649 51 13 3.9 NM 003507 Fzd7 160 41 3.9 NM 001039651 5 C6orf26 19 5 3.8 NM 032961 PCDH10 19 5 3.8 NM 001145199 C12orf75 27 7 3.9 NM 001223 Casp1 27 7 3.9 NM 001128613 NUDT22 61 16 3.8 NM 002648 PIM1 61 16 3.8 NM 003830 SIGLEC5 96 25 3.8 NM 001206665 IGSF8 138 36 3.8 NM 020817 KIAA1407 34 9 3.8 NM 005275 2 GNL1 76 twenty 3.8 NM 007283 MGLL 84 22 3.8 NM 001172624 NE01 126 33 3.8 NM 031909 C1QTNF4 91 24 3.8 NM 003484 Hmga2 49 13 3.8 NM 001102594 Dtx2 79 21 3.8 NM 001031827 1 BOLA2 87 23 3.8 NM 001039182 1 BOLA2B 87 23 3.8 NM 000755 CRAT 132 35 3.8

NM 153697 ANKRD44 fifteen four 3.8 NM 020811 CARNS1 fifteen four 3.8 NM 013246 CLCF1 fifteen four 3.8 NM 001882 CRHBP fifteen four 3.8 NM 005232 EPHA1 90 24 3.8 NM 145059 FUK 60 16 3.8 NM 022041 Gan 67 eighteen 3,7 NM 002048 GAS1 fifteen four 3.8 NR 036632 GOLGA2B 22 6 3,7 NM 001004439 ITGA11 fifteen four 3.8 NM 139248 Liph fifteen four 3.8 NM 001025101 MBP 75 twenty 3.8 NR 026902 MGC16142 fifteen four 3.8 NR 040072 NDRG4 22 6 3,7 NM 012387 Padi4 fifteen four 3.8 NM 001195263 PDZD7 45 12 3.8 NM 001080492 PRSS54 fifteen four 3.8 NM 213613 SLC29A4 fifteen four 3.8 NM 000455 STK32C fifteen four 3.8 NM 001144923 TTC28-AS1 37 10 3,7 NM 198310 TUBB2A 52 fourteen 3,7 NM 001039886 ZNF837 22 6 3,7 NM 001128302 LYRM1 78 21 3,7 NM 001129979 SYNE1 70 19 3,7 NM 001114185 MVK 48 13 3,7 NM 000920 PC 154 42 3,7 NM 001122674 ABCD3 33 9 3,7 NM 001173539 BAN Ρ 33 9 3,7 NM 199248 CACNB1 33 9 3,7 NM 052843 OBSCN 99 27 3,7 NM 194250 ZNF816-ZNF321P 66 eighteen 3,7 NM 032290 ANKRD32 124 34 3.6 NM 014726 TBX2 58 16 3.6 NM 031213 FAM108A1 135 37 3.6 NM 001005855 ATP8B2 25 7 3.6 NM 173550 C9orf93 51 fourteen 3.6 NM 006531 IFT88 25 7 3.6 NM 178496 MB21D2 51 fourteen 3.6 NR 037429 MIR3656 25 7 3.6 NM 031430 Rilp 51 fourteen 3.6 NM 080861 SPTBN4 25 7 3.6 NM 001025616 ARHGAP24 43 12 3.6 NM 058195 CDKN2A 217 60 3.6 NR 033742 FKSG43 43 12 3.6 NM 018485 Gpr77 43 12 3.6 NM 003259 ICAM5 61 17 3.6 NM 006995 BTN2A2 97 27 3.6 NM 004110 Fdxr 97 27 3.6 NM 138420 AHNAK2 126 35 3.6 NM 152326 ANKRD9 36 10 3.6 NM 003568 ANXA9 54 fifteen 3.6 NM 004925 AQP3 eighteen 5 3.6 NM 032266 C2orf16 eighteen 5 3.6 NM 016243 CYB5R1 36 10 3.6

NM 001136265 IFF02 54 fifteen 3.6 NM 014994 MAPKBP1 54 fifteen 3.6 NR 031592 MIR1181 eighteen 5 3.6 NM 207127 Paox eighteen 5 3.6 NM 015149 RGL1 eighteen 5 3.6 NM 001080496 RGP1 54 fifteen 3.6 NR 002312 RPPH1 36 10 3.6 NM 015278 SASH1 72 twenty 3.6 NM 001099435 SPINT1 72 twenty 3.6 NM 003608 Gpr65 118 33 3.6 NM 004269 MED27 82 23 3.6 NM 024108 TREML2 82 23 3.6 NM 001170957 Smo 64 eighteen 3.6 NM 002663 Pld2 46 13 3,5 NM 001242788 BRF1 150 42 3.6 NM_003560 PLA2G6 103 29th 3.6 NM 005481 MED16 349 98 3.6 NM_003916 AP1S2 28 8 3,5 NM 004952 Efna3 28 8 3,5 NM_014872 ZCWPW1 57 16 3.6 NM 033375 MY01C 39 eleven 3,5 NM 001171091 UEVLD 49 fourteen 3,5 NM 000584 IL8 172 49 3,5 NM 000154 Galk1 274 78 3,5 NM 001145128 AKD1 10 3 3.3 NM 001109938 6 C6orf136 10 3 3.3 NM 006566 CD226 10 3 3.3 NR 023386 CROCCP3 10 3 3.3 NR 037860 2 EGFL8 10 3 3.3 NM 025193 HSD3B7 10 3 3.3 NM 003866 INPP4B 31 9 3.4 NR 031598 MIR1229 10 3 3.3 NR 036199 MIR4315-1 10 3 3.3 NR 036271 MIR4315-2 10 3 3.3 NR 030369 MIR639 10 3 3.3 NM 001114132 NBEAL1 52 fifteen 3,5 NM 178140 PDZD2 10 3 3.3 NM 001190728 RALGPS1 10 3 3.3 NR 033396 SLC22A20 10 3 3.3 NM 001199693 SLC4A8 10 3 3.3 NM 032038 SPSB2 42 12 3,5 NM 033278 TRIM39 21 6 3,5 NM 021253 5 TRIM39-RPP21 10 3 3.3 NM 152736 ZNF200 129 37 3,5 NM 001204299 ZNF668 76 22 3,5 NM 020703 AMIG01 66 19 3,5 NM 024816 RABEP2 66 19 3,5 NM 001134492 HS2ST1 121 35 3,5 NM 001170881 Gpr137 45 13 3,5 NM 006929 3 SKIV2L 45 13 3,5 NR 002451 ABCA11P 103 thirty 3.4 NM 004913 C16orf7 103 thirty 3.4 NM 152742 Gpc2 34 10 3.4 NM 004761 3 RGL2 34 10 3.4

NM 000048 ASL 93 27 3.4 NM 001204240 TBC1D16 151 44 3.4 NM 001200001 NOTCH2 327 95 3.4 NM 001042432 CLN3 58 17 3.4 NM 001172780 LRRC34 58 17 3.4 NM 130786 A1BG 24 7 3.4 NM 014885 ANAPC10 48 fourteen 3.4 NM 001723 DST 24 7 3.4 NM 004463 Fgd1 48 fourteen 3.4 NM 022107 4 GPSM3 24 7 3.4 NM 001160166 HRH4 24 7 3.4 NM 000428 LTBP2 24 7 3.4 NR 003189 SYTL3 24 7 3.4 NM 007261 CD300A 123 36 3.4 NM 058180 C21orf58 150 44 3.4 NM 014675 Crocc 112 33 3.4 NM 006519 DYNLT1 75 22 3.4 NM 002926 Rgs12 265 78 3.4 NM 002333 Lrp3 295 87 3.4 NM 152504 C20orf196 78 23 3.4 NM 005246 Fer 78 23 3.4 NM 001173474 1 ASMTL 91 27 3.4 NM 145691 ATPAF2 67 twenty 3.4 NM 001012502 C9orf117 13 four 3.3 NM 024705 DHRS12 27 8 3.4 NR 034098 Eml2 54 16 3.4 NM 001001794 FAM116B 13 four 3.3 NM 032301 Fbxw9 40 12 3.3 NR 015369 FLJ42709 13 four 3.3 NM 207647 FSD1L 40 12 3.3 NM 174942 GAS2L3 13 four 3.3 NM 178231 ICA1L 13 four 3.3 NM 002204 ITGA3 27 8 3.4 NM 153487 MDGA1 13 four 3.3 NM 175058 PLEKHA7 13 four 3.3 NM 181808 Poln 13 four 3.3 NM 181882 PRX 40 12 3.3 NM 032167 RUNDC2A 13 four 3.3 NR 033801 SLFNL1 13 four 3.3 NM 178509 SUN1 67 twenty 3.4 NM 001042463 TMEM92 27 8 3.4 NM 031434 TNFRSF11A 13 four 3.3 NM 015516 TSPAN15 67 twenty 3.4 NM 007191 Wnt11 13 four 3.3 NM 145288 ZNF311 13 four 3.3 NM 001243241 ZNF345 27 8 3.4 NM 020928 ZYG11A 13 four 3.3 NM 001099220 ZNHIT2 124 37 3.4 NM 014806 RUSC2 181 54 3.4 NM 001164115 Cass4 70 21 3.3 NM 173540 FUT11 130 39 3.3 NM 001033045 Gpr155 43 13 3.3 NM 053053 TAF13 43 13 3.3 NM 001720 BMP8B 60 eighteen 3.3

NM 152759 LRRC43 thirty 9 3.3 NM 015016 MAST3 120 36 3.3 NM 024578 OCEL1 60 eighteen 3.3 NR 024525 Fam96b 166 fifty 3.3 NM 033340 Casp7 106 32 3.3 NM 015157 PHLDB1 123 37 3.3 NM 016148 SHANK1 142 43 3.3 NM 001142501 MON1A 129 39 3.3 NM 080622 ABHD16B 33 10 3.3 NM 016584 IL23A 16 5 3.2 NM 001010844 IRAK1BP1 16 5 3.2 NM 001040624 NCALD 33 10 3.3 NM 017699 SIDT1 33 10 3.3 NM 213618 ST6GALNAC2 16 5 3.2 NR 002331 STAC 16 5 3.2 NM 001198526 Tchh 16 5 3.2 NM 052883 TXNRD3NB 16 5 3.2 NM 001164267 3 WDR53 132 40 3.3 NM 001214906 ZNF484 33 10 3.3 NM 006646 WBP11P1 102 31 3.3 NM 016481 C9orf156 85 26 3.3 NM 201446 EGFL7 447 136 3.3 NM 001159391 GUK1 190 58 3.3 NM 146387 MRPL4 279 85 3.3 NM 198475 FAM171A2 88 27 3.3 NM 019121 LRRC68 88 27 3.3 NM 001321 CSRP2 36 eleven 3.3 NM 134324 TBC1D10A 36 eleven 3.3 NM 007108 TCF7L2 72 22 3.3 NM 001145845 ROB01 219 67 3.3 NM 024585 ARMC7 130 40 3.3 NM 006123 IDS 75 23 3.3 NM 172251 MRPL54 189 58 3.3 NR 033488 5 ABHD16A 19 6 3.2 NM 052854 CREB3L1 19 6 3.2 NM 004137 KCNMB1 19 6 3.2 NM 004140 LLGL1 97 thirty 3.2 NM 058169 LOH12CR1 39 12 3.3 NM 002461 MVD 78 24 3.3 NM 019080 Ndfip2 19 6 3.2 NM 001135750 PSMG4 19 6 3.2 NR 024284 Zfhx2 19 6 3.2 NM 005529 Hspg2 844 260 3.2 NM 001003692 ZNF133 one hundred 31 3.2 NM 183241 C9orf142 285 88 3.2 NM 018110 DOK4 187 58 3.2 NM 017679 BCAS3 126 39 3.2 NM 001064 TLCD1 42 13 3.2 NM 175630 DNMT3A 64 twenty 3.2 NM 001002913 PTRH1 64 twenty 3.2 NM 024591 CHMP6 222 69 3.2 NR 027271 C19orf23 22 7 3,1 NM 001080849 DNLZ 48 fifteen 3.2 NM 014449 Gpr162 48 fifteen 3.2

NM 006423 RABAC1 48 fifteen 3.2 NM 020145 SH3GLB2 121 38 3.2 NM 015681 B9D1 25 8 3,1 NM 024731 KLHL36 127 40 3.2 NM 178817 M RAP 25 8 3,1 NM 001144978 MTHFD2L 25 8 3,1 NM 016606 REEP2 25 8 3,1 NM 014442 SIGLEC8 25 8 3,1 NM 001168247 SMAGP 25 8 3,1 NM 005928 Mfge8 181 57 3.2 NM 022489 INF2 312 98 3.2 NM 001031714 INF2 312 98 3.2 NM 001198897 ACY1 105 33 3.2 NR 037505 MIR3940 54 17 3.2 NM 213598 ZNF552 54 17 3.2 NM 053064 GNG2 333 105 3.2 NM 001199580 SUV420H2 139 44 3.2 NM 001166209 Sys1 196 62 3.2 NM 015407 ABHD14A 85 27 3,1 NM 130781 RAB24 85 27 3,1 NM 018383 Wdr46 57 eighteen 3.2 NM 145249 IFI27L1 60 19 3.2 NM 032335 PHF6 91 29th 3,1 NM 001008486 SLC44A1 91 29th 3,1 NM 024832 RIN3 246 78 3.2 NM 001164638 Endov 31 10 3,1 NR 027712 PIP5K1P1 31 10 3,1 NM 018363 RNLS 63 twenty 3.2 NM 003239 Thap2 31 10 3,1 NM 014008 Ccdc22 129 41 3,1 NM 001100418 C19orf60 97 31 3,1 NM 001733 C1R 97 31 3,1 NM 003098 SNX33 66 21 3,1 NM 006427 SIVA1 232 74 3,1 NM 001040134 Palm 166 53 3,1 NM 030945 C1QTNF3 34 eleven 3,1 NM 080750 DPH3P1 34 eleven 3,1 NM 001163213 FGFR3 34 eleven 3,1 NM 153835 Gpr113 34 eleven 3,1 NM 001144925 MX1 34 eleven 3,1 NR 033799 2 NSFP1 34 eleven 3,1 NM 014596 2 Zp3 138 44 3,1 NM 005711 EDIL3 106 34 3,1 NM 033400 Zfp41 147 47 3,1 NM 001159846 MPND 78 25 3,1 NM 012121 CDC42EP4 196 63 3,1 NM 173674 DCBLD1 40 13 3,1 NM 022772 EPS8L2 40 13 3,1 NM 001099280 HEATR7A 40 13 3,1 NM 016619 PLAC8 81 26 3,1 NM 004920 Aatk 124 40 3,1 NM 031454 SELO 168 54 3,1 NM 032626 RBBP6 127 41 3,1 NM 001198559 DNAJC27 87 28 3,1

NM 006233 POLR2I 43 fourteen 3,1 NM 022903 Ccdc71 267 86 3,1 NM 001001662 ZNF787 273 88 3,1 NM 033513 C19orf20 46 fifteen 3,1 NM 203339 CLU 96 31 3,1 NR 003672 SNHG7 618 200 3,1 NM 003965 CCRL2 52 17 3,1 NM 030943 Amn 55 eighteen 3,1 NM 013382 POMT2 55 eighteen 3,1 NM 000101 CYBA 345 112 3,1 NM 016194 GNB5 58 19 3,1 NM 001040126 PQLC2 58 19 3,1 NM 203374 ZNF792 58 19 3,1 NM 001003677 C11orf49 61 twenty 3,1 NM 032775 KLHL22 123 40 3,1 NM 001006643 SLC25A29 255 83 3,1 NM 001102367 C14orf159 258 84 3,1 NM 198988 LENG9 129 42 3,1 NM 005583 Lyl1 1443 470 3,1 NM 138559 BCL11A 132 43 3,1 NM 006848 CCDC85B 531 173 3,1 NM 144589 COMTD1 67 22 3.0 NM 173628 DNAH17 82 27 3.0 NM 005608 PTPRCAP 262 86 3.0 NM 016113 TSHZ3 109 36 3.0 NM 001199694 SLC5A2 115 38 3.0 NM 017723 C9orf167 534 176 3.0 NM 002335 Lrp5 486 161 3.0 NM 006675 Tspo 771 256 3.0 NM 001039705 TRPM2 493 164 3.0 NM 181814 ABHD12B fifteen 5 3.0 NM 080658 ACY3 12 four 3.0 NM 001107 ACYP1 24 8 3.0 NM 025008 ADAMTSL4 33 eleven 3.0 NR 037186 APITD1-CORT 36 12 3.0 NM 021822 APOBEC3G 240 80 3.0 NM 033515 ARHGAP18 48 16 3.0 NM 032131 ARMC2 fifteen 5 3.0 NM 004655 Axin2 eighteen 6 3.0 NR 037658 BLOC1S1-RDH5 111 37 3.0 NR 026566 BMS1P1 12 four 3.0 NR 003611 BMS1P5 12 four 3.0 NM 001145033 C11orf96 12 four 3.0 NM 032829 C12orf34 12 four 3.0 NM 001017923 C14orf28 fifteen 5 3.0 NM 001012984 C16orf86 fifteen 5 3.0 NM 152914 C17orf103 36 12 3.0 NM 024323 C19orf57 thirty 10 3.0 NM 032261 C21orf56 24 8 3.0 NM 001013717 C5orf56 eighteen 6 3.0 NM 001747 Capg 48 16 3.0 NM 004055 Capn5 27 9 3.0 NM 001130726 CCDC149 27 9 3.0 NM 001105564 1 CCHCR1 9 3 3.0

NM 006779 CDC42EP2 12 four 3.0 NM 001819 CHGB 36 12 3.0 NM 052999 CMTM1 12 four 3.0 NM 006314 CNKSR1 fifteen 5 3.0 NM 001171174 CX3CR1 12 four 3.0 NM 019074 Dll4 9 3 3.0 NM 001934 DLX4 12 four 3.0 NM 017833 DNAJC28 9 3 3.0 NR 036554 DPY19L2P3 9 3 3.0 NM 024712 ELM03 27 9 3.0 NM 007046 EMILIN1 fifteen 5 3.0 NM 207582 ERVFRD-1 eighteen 6 3.0 NM_001105517 FAM58BP 9 3 3.0 NM__014808 FARP2 69 23 3.0 NM 033449 Fchsd1 177 59 3.0 NR 033904 FLJ39639 12 four 3.0 NM 001459 FLT3LG fifteen 5 3.0 NM 022158 Fn3k eighteen 6 3.0 NR 034096 FONG 24 8 3.0 NM 005252 Fos 33 eleven 3.0 NM 005438 Fosl1 fifteen 5 3.0 NM 003923 Foxh1 eighteen 6 3.0 NM 001112808 FPGT-TNNI3K 12 four 3.0 NM 139285 GAS2L2 9 3 3.0 NM 021954 Gja3 eighteen 6 3.0 NR 003945 Gvinp1 9 3 3.0 NR 027716 H2AFJ 45 fifteen 3.0 NM 019111 6 Hla-dra 9 3 3.0 NM 178582 Hm13 114 38 3.0 NM 001098213 HRH1 24 8 3.0 NM 003853 IL18RAP fifteen 5 3.0 NM 005101 ISG15 57 19 3.0 NM 002291 Lamb1 39 13 3.0 NM 001003679 Lepr 9 3 3.0 NM 030891 LRRC3 54 eighteen 3.0 NM 002348 Ly9 thirty 10 3.0 NM 181644 Mfs4 eighteen 6 3.0 NR 003682 MGC70870 9 3 3.0 NR 002208 MRPL42P5 9 3 3.0 NM 178170 NEK8 51 17 3.0 NM 002507 NGFR 12 four 3.0 NM 004387 NKX2-5 12 four 3.0 NM 002593 PCOLCE 9 3 3.0 NM 148172 PEMT 12 four 3.0 NM 004813 Pex16 66 22 3.0 NM 001185181 3 PFDN6 27 9 3.0 NM 058237 PPP4R4 12 four 3.0 NR 037861 5 PPT2-EGFL8 eighteen 6 3.0 NM 001160168 PRR5L 72 24 3.0 NM 003978 PSTPIP1 36 12 3.0 NM 152882 PTK7 27 9 3.0 NM 001013658 PTX4 fifteen 5 3.0 NM 007023 RAPGEF4 eighteen 6 3.0 NM 017805 RAS IP 1 12 four 3.0

NM 001204513 RBAK-LOC389458 fifteen 5 3.0 NM 006871 Ripk3 eighteen 6 3.0 NR 003051 RMRP 12 four 3.0 NM 152470 RNF165 12 four 3.0 NR 026982 RPL23AP82 48 16 3.0 NM 005226 S1PR3 39 13 3.0 NR 003023 SCARNA2 12 four 3.0 NM 003020 SCG5 12 four 3.0 NM 020796 SEMA6A fifteen 5 3.0 NR 003032 SNORA40 thirty 10 3.0 NR 002973 SNORA57 eighteen 6 3.0 NM 004710 SYNPO thirty 10 3.0 NM 033542 SYT11 thirty 10 3.0 NM 001199107 TBC1D29 12 four 3.0 NM 080647 TBX21 9 3 3.0 NM 007170 Tex9 9 3 3.0 NM 001097599 TMEM221 36 12 3.0 NM 033227 TRIM26 66 22 3.0 NM 001160389 TRPV1 42 fourteen 3.0 NM 001145338 3 ZBTB26 66 22 3.0 NM 005453 1 ZBTB37 eighteen 6 3.0 NR 024091 ZFYVE28 21 7 3.0 NM 198087 ZNF221 thirty 10 3.0 NM 001039654 ZNF571 21 7 3.0 NM 173658 ZNF665 12 four 3.0 NM 021216 ZNF713 21 7 3.0 NM 181877 ZSCAN21 162 54 3.0 NM 001006642 SLC25A6 2143 718 3.0 NM 020158 EXOSC5 211 71 3.0 NM 014516 CNOT3 193 65 3.0 NM 000932 PLCB3 369 124 3.0 NM 203458 NOTCH2NL 178 60 3.0 NM 001171907 CXorf40A 130 44 3.0 NM 024812 Baalc 124 42 3.0 NM 017797 BTBD2 483 163 3.0 NM 001039842 C17orf90 240 81 3.0 NM 001012759 CTU2 103 35 2.9 NM 001003791 Erlin2 one hundred 34 2.9 NM 176792 MRPL43 one hundred 34 2.9 NM 024618 NLRX1 277 94 2.9 NM 013304 ZDHHC12 159 54 2.9 NM 004421 DVL1 232 79 2.9 NM 033397 ITPRIP 153 52 2.9 NM 022092 CHTF18 223 76 2.9 NM 001014811 ME3 73 25 2.9 NM 004808 NMT2 73 25 2.9 NM 012230 POMZP3 73 25 2.9 NM 025076 Vars 73 25 2.9 NM 033046 RTKN 144 49 2.9 NM 001172669 ZNF672 211 72 2.9 NM 018686 CMAS 205 70 2.9 NM 003839 TNFRSF14 67 23 2.9 NM 001142651 NEURL1B 132 45 2.9 NM 002856 PVRL2 196 67 2.9

NM 003562 SLC25A11 243 83 2.9 NM 006951 Tanc1 120 41 2.9 NM 000201 ICAM1 237 81 2.9 NM 001170538 DZIP1L 58 twenty 2.9 NM 032305 POLR3GL 231 79 2.9 NM 000029 AGT 111 38 2.9 NM 001044264 MSMP 55 19 2.9 NM 001160116 C15orf40 52 eighteen 2.9 NM 006653 FRS3 52 eighteen 2.9 NR 026787 Lmln 52 eighteen 2.9 NM 020855 ZNF497 52 eighteen 2.9 NM 033054 MY01G 1294 445 2.9 NM 001005910 IP6K2 142 49 2.9 NR 039964 MIR4800 46 16 2.9 NM 019020 TBC1D24 183 63 2.9 NM 144998 STX10 133 46 2.9 NM 005516 Hla-e 307 106 2.9 NM 005575 Lnpep 217 75 2.9 NM 152519 C2orf67 127 44 2.9 NM 138790 PLD4 787 272 2.9 NM 004672 MAP3K6 40 fourteen 2.9 NM 016354 SLITRK5 321 111 2.9 NM 024668 ANKHD1 312 108 2.9 NM 001204880 UBE2F 78 27 2.9 NM 001164623 EIF2C2 349 121 2.9 NM 001006622 Wdr34 228 79 2.9 NM 006478 GAS2L1 37 13 2,8 NR 024154 NUDT7 75 26 2.9 NM 145291 ZC3H10 150 52 2.9 NM 001007523 1 F8A2 147 51 2.9 NM 001007524 1 F8A3 147 51 2.9 NM 001110215 CENPM 109 38 2.9 NM 001039548 KLHL35 109 38 2.9 NM 030665 RAM 219 76 2.9 NM 001010919 Fam26f 106 37 2.9 NM 201265 UBR2 247 86 2.9 NM 001039481 ETNK1 34 12 2,8 NM 001988 EVPL 448 156 2.9 NM 003024 ITSN1 34 12 2,8 NM 198439 KBTBD3 34 12 2,8 NM 001142627 SEC61A2 34 12 2,8 NM 001004351 SPDYE6 34 12 2,8 NM 024100 Wdr20 103 36 2.9 NM 002751 MAPK11 273 95 2.9 NM 032341 DDI2 169 59 2.9 NR 039900 MIR4745 one hundred 35 2.9 NM 001172702 SLC38A7 one hundred 35 2.9 NM 012100 DNPEP 232 81 2.9 NM 001143915 Fam76a 66 23 2.9 NM 172208 3 Tapbp 163 57 2.9 NR 039872 MIR4721 97 34 2.9 NM 004424 E4f1 129 45 2.9 NM 001164603 ASXL1 31 eleven 2,8 NM 031300 MXD3 94 33 2,8

NR 026963 Ttc 8 31 eleven 2,8 NM 182587 UNCX 31 eleven 2,8 NM 022362 MMS19 217 76 2.9 NM 020342 SLC39A3 432 151 2.9 NM 032306 ALKBH7 240 84 2.9 NM. 002746 MAPK3 120 42 2.9 NM 024083 ASPSCR1 228 80 2.9 NM 024296 CCDC28B 114 40 2.9 NM 001134759 CRYZ 85 thirty 2,8 NM 001024948 Fnbp1l 85 thirty 2,8 NM_015660 GIMAP2 285 one hundred 2.9 NM 002263 KIFC1 142 fifty 2,8 NM 001146210 SPNS1 85 thirty 2,8 NM 001017964 YIF1B 171 60 2.9 NM 012197 RABGAP1 253 89 2,8 NM 032591 SLC04A1 253 89 2,8 NM 005569 LIMK2 196 69 2,8 NM 007097 CLTB 222 78 2,8 NM 002415 MIF 936 329 2,8 NM 181336 Lemd2 247 87 2,8 NM 001013436 Mpst 355 125 2,8 NM 018477 ACTR10 136 48 2,8 NM 020719 PRR12 298 105 2,8 NM 175871 C19orf39 54 19 2,8 NR 024348 FBXL19-AS1 54 19 2,8 NM 005668 STAP1 108 38 2,8 NM 001174158 Zfp1 162 57 2,8 NM 006454 MXD4 640 226 2,8 NM 014272 ADAMTS7 25 9 2,8 NM 020785 CC2D2A 25 9 2,8 NM 024553 Ccdc132 25 9 2,8 NM 032309 CHCHD5 25 9 2,8 NM 176782 FAM151A 76 27 2,8 NM 022368 Pja1 76 27 2,8 NM 001017369 SC4MOL 51 eighteen 2,8 NM 022968 SERF1A 25 9 2,8 NM 001178087 1 SERF1B 25 9 2,8 NR 000018 SNORD68 25 9 2,8 NM 013351 Tcea2 25 9 2,8 NM 001136053 Tpst2 76 27 2,8 NM 199282 ARHGAP27 226 80 2,8 NM 016073 HDGFRP3 147 52 2,8 NM 006236 POU3F3 73 26 2,8 NM 032271 TRAPPC1 147 52 2,8 NM 022060 ABHD4 1122 397 2,8 NM 001128917 Tor2a 195 69 2,8 NM 152441 Fbxl14 169 60 2,8 NR 027308 MEF2BNB-MEF2B 48 17 2,8 NM 203473 Porcn 70 25 2,8 NM 019848 SLC10A3 163 58 2,8 NM 001199173 MLST8 186 66 2,8 NR 026962 TTC39C 93 33 2,8 NM 005597 Nfic 205 73 2,8 NM 014603 CDR2L 90 32 2,8

NR 002174 CMAHP 22 8 2,8 NM 001114137 EPB49 22 8 2,8 NM 019013 Fam64a 112 40 2,8 NM 012227 1 Gtbp6 45 16 2,8 NM 014181 HSPC159 45 16 2,8 NM 002373 MAPI A 22 8 2,8 NM 004556 Nfkbie 247 88 2,8 NM 001130102 NR1H3 22 8 2,8 NM 001164721 Ptafr 22 8 2,8 NM 021033 RAP2A 22 8 2,8 NM 023074 ZNF655 112 40 2,8 NM 003775 S1PR4 492 175 2,8 NM 003088 FSCN1 685 244 2,8 NM 001203264 IL17RC 87 31 2,8 NM 003793 Ctsf 213 76 2,8 NM 020856 TSNARE1 106 38 2,8 NM 032898 Cep19 42 fifteen 2,8 NR 037909 ARHGAP19-SLIT1 103 37 2,8 NM 001197330 POLE2 61 22 2,8 NM 201400 Fam86a 181 65 2,8 NM 202494 Gipc1 159 57 2,8 NM 022810 SLC25A25 198 71 2,8 NM 001110792 MECP2 276 99 2,8 NM 133450 ANKS3 39 fourteen 2,8 NM 001725 BPI 19 7 2.7 NM 152343 C17orf46 19 7 2.7 NM 178868 CMTM8 78 28 2,8 NM 021958 Hlx 19 7 2.7 NR 027145 LIMS3-LOC440895 19 7 2.7 NM 002332 Lrp1 19 7 2.7 NM 001080401 PPM1N 19 7 2.7 NM 001204103 1 PPT2 19 7 2.7 NM 002931 Ring1 58 21 2,8 NR 002789 SEPHS1P 19 7 2.7 NR 003033 SNORD88B 19 7 2.7 NM 001168316 ULBP2 19 7 2.7 NM 001206802 TPRA1 400 144 2,8 NM 205767 C19orf70 114 41 2,8 NM 018273 TMEM160 75 27 2,8 NM 005452 2 Wdd67 205 74 2,8 NM 130791 Xab2 411 148 2,8 NM 001025108 AFF3 55 twenty 2,8 NM 005740 DNAL4 55 twenty 2,8 NM 002626 Pfkl 1123 405 2,8 NM 001042600 MAP4K1 183 66 2,8 NM 000052 ATP7A 36 13 2,8 NM 175709 CBX7 72 26 2,8 NR 037775 MIA-RAB4B 36 13 2,8 NM 005861 STX16-NPEPL1 465 168 2,8 NM 001207056 DNMT3B 124 45 2,8 NM 001202557 CD44 616 223 2,8 NM 004639 5 BAG6 105 38 2,8 NM 152485 C1orf74 52 19 2.7 NM 001823 Hard currency 525 190 2,8

NR 027063 NCRNA00116 52 19 2.7 NM 205842 NCKAP1 121 44 2,8 NM 017728 TMEM115 328 119 2,8 NM 004765 BCL7C 69 25 2,8 NR 003024 EIF3IP1 171 62 2,8 NM 198900 FMNL3 204 74 2,8 NM 015321 CRTC1 237 86 2,8 NM 001048172 MUTYH 151 55 2.7 NM 206967 C16orf74 16 6 2.7 NM 021267 CERS1 33 12 2,8 NM 138481 Chadl 16 6 2.7 NM 001492 Gdf1 33 12 2,8 NR 003504 Gusbp2 16 6 2.7 NM 005345 HSPA1A 49 eighteen 2.7 NM 032123 KIRREL2 16 6 2.7 NR 037408 MIR3614 16 6 2.7 NR 027775 PMS2P5 33 12 2,8 NM 001198952 RNF103 66 24 2,8 NM 001172109 Senp8 16 6 2.7 NM 018656 SLC38A6 16 6 2.7 NR 034178 Ssc5d 16 6 2.7 NM 000593 4 Tap2 33 12 2,8 NM 018073 TRIM8 264 96 2,8 NM 145294 Wif1 16 6 2.7 NM 001160407 TMED1 327 119 2.7 NM 018140 Cep72 291 106 2.7 NM 004285 H6PD 387 141 2.7 NM 138961 ESAM 126 46 2.7 NR 033322 NSUN5P1 126 46 2.7 NM 001130848 PITPNM1 391 143 2.7 NM 007255 B4GALT7 109 40 2.7 NM 017828 COMMD4 219 80 2.7 NM 015094 Hic2 219 80 2.7 NM 006666 RUVBL2 561 205 2.7 NM 152916 EMR2 46 17 2.7 NM 020704 Fam40b 46 17 2.7 NM 001127396 SUGP1 279 102 2.7 NM 015356 Scrib 478 175 2.7 NM 018340 CPPED1 169 62 2.7 NM 173793 C22orf39 123 45 2.7 NR 037425 MIR3652 123 45 2.7 NM 004204 Pigq 76 28 2.7 NM 020722 KIAA1211 286 105 2.7 NR 037846 1 MSH5-C6orf26 thirty eleven 2.7 NM 001171906 NUDT16 thirty eleven 2.7 NM 007280 OIP5 60 22 2.7 NM 001135610 PRDM2 thirty eleven 2.7 NR 027662 SLC25A14 thirty eleven 2.7 NM 001032292 ZNF187 90 33 2.7 NM 001687 ATP5D 223 82 2.7 NM 001080826 SGK223 133 49 2.7 NM 001171888 FGFR10P2 103 38 2.7 NM 152299 NCAPH2 427 157 2.7 NM 032402 PCDHGC3 73 27 2.7

NM 001005362 DNM2 1083 398 2.7 NM 031215 Cables2 117 43 2.7 NM 203370 C3orf54 394 145 2.7 NM 001077685 AGAP7 43 16 2.7 NM 001007237 IGSF3 87 32 2.7 NM 000442 Pecam1 43 16 2.7 NM 001122764 PPOX 87 32 2.7 NR 002818 RNF126P1 43 16 2.7 NM 016504 MRPL27 285 105 2.7 NM 206901 RTN2 57 21 2.7 NM 001024 RPS21 469 173 2.7 NM 020812 DOCK6 198 73 2.7 NM 012181 FKBP8 1068 394 2.7 NM 182984 TRNAU1AP 84 31 2.7 NM 015603 Ccdc9 97 36 2.7 NM 006339 Hmg20b 400 148 2.7 NR 024332 POMGNT1 165 61 2.7 NM 009590 Aoc2 13 5 2.6 NM 001130414 APBA2 13 5 2.6 NM 004052 Bnip3 67 25 2.7 NM 024650 C11orf80 27 10 2.7 NR 029406 FLJ43681 13 5 2.6 NM 000238 KCNH2 13 5 2.6 NM 001194958 KCNJ18 27 10 2.7 NM 033402 LRRCC1 67 25 2.7 NM 020142 NDUFA4L2 94 35 2.7 NM 022141 PARVG 270 one hundred 2.7 NM 144579 SFXN5 13 5 2.6 NM 013356 SLC16A8 27 10 2.7 NM 003172 SYCE1L 13 5 2.6 NM 001195227 TRPT1 108 40 2.7 NM 003312 Ttc14 67 25 2.7 NM 016030 Ttc23 40 fifteen 2.7 NM 001184801 UBTD1 13 5 2.6 NM 007130 ZNF414 54 twenty 2.7 NM 001039140 C20orf27 429 159 2.7 NM 139355 MATK 402 149 2.7 NM 024037 C1orf135 267 99 2.7 NM 006913 6 Rnf5 91 34 2.7 NM 021222 PRUNE 169 63 2.7 NR 024063 ZSCAN20 78 29th 2.7 NM 152727 CPNE2 363 135 2.7 NM 025256 6 Ehmt2 64 24 2.7 NM 005343 H RAS 129 48 2.7 NM 032477 MRPL41 64 24 2.7 NM 001145441 UBE2D1 129 48 2.7 NM 002401 MARZKZ 682 254 2.7 NM 001085365 MZT2A 268 one hundred 2.7 NM 001001976 ATE1 102 38 2.7 NM 005346 3 HSPA1B 51 19 2.7 NM 024112 C9orf16 177 66 2.7 NM 002028 Fntb 88 33 2.7 NM 013265 C11orf2 892 333 2.7 NM 024872 DOK3 313 117 2.7

NM 020445 ACTR3B 75 28 2.7 NM 006020 ALKBH1 75 28 2.7 NM 001193529 CCT6B 37 fourteen 2.6 NM 001018112 Fanca 75 28 2.7 NM 001199162 USP40 112 42 2.7 NM 000033 ABCD1 174 65 2.7 NM 012200 B3gat3 174 65 2.7 NM 145030 C7orf47 235 88 2.7 NM 014386 PKD2L2 61 23 2.7 NM 001190737 Nfib 85 32 2.7 NM 012454 Ticam1 85 32 2.7 NM 153497 TADA1 133 fifty 2.7 NM 006384 Cib1 301 113 2.7 NM 001190727 C9orf103 72 27 2.7 NM 152361 Eid2b 24 9 2.7 NM 024519 Fam65a 72 27 2.7 NM 001144829 FM05 24 9 2.7 NR 024206 NCRNA00152 120 45 2.7 NM 004822 NTN1 168 63 2.7 NM 181515 MRPL21 250 94 2.7 NM 003149 STARD8 189 71 2.7 NM 022104 PCIF1 364 137 2.7 NM 001242758 5 H LA-A 2173 817 2.7 NM 181718 ASPHD1 58 22 2.6 NM 001083314 CHMP1A 409 154 2.7 NM 020888 KIAA1522 58 22 2.6 NM 001164507 NEB 58 22 2.6 NM 014700 RAB11FIP3 58 22 2.6 NM 001143784 Fes 840 316 2.7 NM 004260 RECQL4 778 293 2.7 NM 004938 DAPK1 382 144 2.7 NM 016274 PLEKH01 379 143 2.7 NM 031209 QTRT1 172 65 2.6 NM 001080821 ZNF808 34 13 2.6 NM 012477 WBSCR16 331 125 2.6 NM 002586 5 PBX2 148 56 2.6 NM 080546 SLC45A3 148 56 2.6 NM 017607 PPP1R12C 490 185 2.6 NM 138471 C11orf84 193 73 2.6 NM 001037339 PDE4B 79 thirty 2.6 NM 001164760 PRKAR1B 159 60 2.7 NM 000958 PTGER4 238 90 2.6 NM 002997 SDC1 79 thirty 2.6 NM 145003 Tspan7 79 thirty 2.6 NM 001136200 C10orf32 124 47 2.6 NM 173852 KRTCAP2 124 47 2.6 NM 175573 ADRM1 1014 383 2.6 NM 173575 STMN3 529 200 2.6 NM 025057 C14orf45 45 17 2.6 NM 021083 Xylb 90 34 2.6 NM 004217 Aurkb 211 80 2.6 NM 133261 Gipc3 55 21 2.6 NR 024116 RASA4P 55 21 2.6 NM 001078650 TMEM141 166 63 2.6

NM 144695 Brox 121 46 2.6 NM 001146696 KDM4C 66 25 2.6 NM 001013663 PTRHD1 66 25 2.6 NM 015670 Senp3 483 183 2.6 NM 198723 Tceb2 208 79 2.6 NM 032189 ATP11A 535 203 2.6 NM 173091 NFATC2 153 58 2.6 NM 004073 PLK3 87 33 2.6 NM 004470 FKBP2 97 37 2.6 NM 017884 PINX1 118 45 2.6 NM 017631 DDX60 129 49 2.6 NM_024105 ALG12 150 57 2.6 NM 052951 DNTTIP1 171 65 2.6 NM 024029 ΖΑΚ 811 309 2.6 NM 001013619 AGPHD1 10 four 2.5 NM 019046 ANKRD16 52 twenty 2.6 NM 001143778 ASAP3 10 four 2.5 NM 174983 C19orf28 199 76 2.6 NM 001146310 C1orf86 21 8 2.6 NR 003545 C5orf44 31 12 2.6 NM 018465 C9orf46 52 twenty 2.6 NM 024111 CHAC1 10 four 2.5 NM 014693 ECE2 136 52 2.6 NM 001145053 Efcab5 10 four 2.5 NM 001137610 FAM86B2 10 four 2.5 NM 022110 FKBPL 10 four 2.5 NR 028038 Gats 63 24 2.6 NM 001142853 Hes6 10 four 2.5 NM 138284 IL17D 21 8 2.6 NM 138433 KLHDC7B 21 8 2.6 NM 006992 LRRC23 10 four 2.5 NM 001012755 MCART6 10 four 2.5 NM 172166 7 Msh5 10 four 2.5 NM 025198 MTERFD3 63 24 2.6 NM 020884 MYH7B 10 four 2.5 NR 024260 NECAP1 126 48 2.6 NM 002514 Nov 10 four 2.5 NM 001145939 PAFAH1B3 199 76 2.6 NM 003498 SNORA4 31 12 2.6 NM 080744 SSBP2 10 four 2.5 NM 000660 TGM1 10 four 2.5 NM 001190919 THY1 21 8 2.6 NM 003250 Tiam2 10 four 2.5 NM 020431 TMEM67 10 four 2.5 NM 001142301 TMEM79 63 24 2.6 NM 177556 TRPC1 31 12 2.6 NM 016653 ZBED1 157 60 2.6 NM 031486 ZNF486 42 16 2.6 NM 016536 ZNF585B 10 four 2.5 NM 022744 C16orf58 364 139 2.6 NM 019103 ZMYM3 270 103 2.6 NM 006441 Mthfs 123 47 2.6 NM 175875 Six5 112 43 2.6 NM 001040450 Fam63b 102 39 2.6

NM 001105205 RUSC1 285 109 2.6 NM 001009991 TAB1 91 35 2.6 NM 001080495 TNS1 91 35 2.6 NM 032809 Fam73b 243 93 2.6 NM 152527 SLC16A14 151 58 2.6 NM 153483 IL17RE 70 27 2.6 NM 144638 TMEM55B 211 81 2.6 NM 020328 ACVR1B 391 150 2.6 NM 152400 C4orf32 130 fifty 2.6 NM 001136108 R3HCC1 370 142 2.6 NR 037192 ABHD14A-ACY1 169 65 2.6 NM 153688 ZFP91-CNTF 586 225 2.6 NM 001008566 TRABD 466 179 2.6 NM 173487 C4orf33 49 19 2.6 NR 026914 MGC16275 49 19 2.6 NM 207351 PRRT3 99 38 2.6 NM 001197181 TUBGCP6 495 190 2.6 NM 145914 ZSWIM4 99 38 2.6 NM 001142298 SRBD1 414 159 2.6 NM 001116 ADCY9 88 34 2.6 NM 000211 ITGB2 442 170 2.6 NM 017825 ADPRHL2 255 98 2.6 NM 025065 RPF1 322 124 2.6 NR 003582 ATP8B5P 78 thirty 2.6 NM 177938 P4HTM 78 thirty 2.6 NM 013299 SAC3D1 117 45 2.6 NM 015000 STOX1 39 fifteen 2.6 NM 004626 WWOX 39 fifteen 2.6 NM 004429 Efnb1 67 26 2.6 NM 032641 SPSB3 135 52 2.6 NM 173467 MCAT 163 63 2.6 NM 001005377 PLAUR 96 37 2.6 NM 016260 IKZF2 153 59 2.6 NM 001206682 ATF7 28 eleven 2.5 NM 001206945 Cspg5 28 eleven 2.5 NM 020340 KIAA1244 28 eleven 2.5 NM 003550 Mad1l1 199 77 2.6 NM 031417 MARK4 85 33 2.6 NM 018328 MBD5 28 eleven 2.5 NM 021959 2 PPP1R11 28 eleven 2.5 NM 017999 RNF31 228 88 2.6 NM 052978 TRMT12 57 22 2.6 NM 138425 C12orf57 321 124 2.6 NM 001145312 Etv3 75 29th 2.6 NM 018727 TRPV2 121 47 2.6 NM 152892 LRWD1 168 65 2.6 NM 015710 GLTSCR2 969 375 2.6 NR 026586 Efcab2 46 eighteen 2.6 NM 207370 Gpr153 46 eighteen 2.6 NM 032701 SYDE2 93 36 2.6 NM 001024683 ZNF69 46 eighteen 2.6 NM 018998 Fbxw5 648 251 2.6 NM 002691 POLD1 619 240 2.6 NM 018316 KLHL26 129 fifty 2.6

NM 021620 PRDM13 64 25 2.6 NM 001040457 RHBDD2 258 one hundred 2.6 NM 203385 RNH1 810 314 2.6 NM_139027 ADAMTS13 82 32 2.6 NM 021922 FANCE one hundred 39 2.6 NM 003338 UBE2M 219 85 2.6 NM 003977 Aip 496 193 2.6 NM 207354 ANKRD13D 252 98 2.6 NM 024827 HDAC11 eighteen 7 2.6 NM 001036645 Hhla3 eighteen 7 2.6 NM 015133 MAPK8IP3 234 91 2.6 NR 030366 MIR636 eighteen 7 2.6 NM 138383 MTSS1L 288 112 2.6 NM 012339 TSPAN9 eighteen 7 2.6 NM 002741 PKN1 2068 805 2.6 NM 001105079 Fbrs 187 73 2.6 NM 032214 SLA2 133 52 2.6 NM 001344 Dad1 759 296 2.6 NM 001144872 CCDC42B 166 65 2.6 NR 026868 ANKRD19P 481 188 2.6 NM 001433 ERN1 105 41 2.6 NM 001760 CCND3 148 58 2.6 NM 033446 Fam125b 148 58 2.6 NM 003926 MBD3 508 199 2.6 NM 007074 COR01A 1731 678 2.6 NM 016162 Ing4 145 57 2.5 NM 002532 NUP88 393 154 2.6 NM 001136540 APOL1 51 twenty 2.6 NM 152854 Cd40 25 10 2.5 NM 138782 Fch02 102 40 2.6 NM 001166271 SPDYA 25 10 2.5 NM 001195082 TFEB 25 10 2.5 NM 153028 ZNF775 51 twenty 2.6 NM 007317 KIF22 558 219 2.5 NM 001004309 ZNF777 295 116 2.5 NM 080669 SLC4A1AP 270 106 2.5 NM 018263 ASXL2 1270 499 2.5 NM 001168303 KLHL13 84 33 2.5 NM 003321 TXNRD2 84 33 2.5 NM 003190 1 TARBP2 226 89 2.5 NM 030587 B4GALT2 259 102 2.5 NM 001136197 Fzr1 249 98 2.5 NM 017566 KLHDC4 124 49 2.5 NM 001243014 ABCB9 33 13 2.5 NM 001099407 C20orf12 66 26 2.5 NM 173589 DNHD1 66 26 2.5 NR 036049 1 MIR1184-1 99 39 2.5 NR 036259 1 MIR1184-2 99 39 2.5 NR 036260 1 MIR1184-3 99 39 2.5 NM 001170944 PNMA6C 33 13 2.5 NM 002831 PTPN6 429 169 2.5 NM 002935 RNASE3 33 13 2.5 NR 003 129 7 RNF5P1 198 78 2.5 NM 001143676 SGK1 33 13 2.5

NM 001040657 Ttc26 33 13 2.5 NM 005706 TSTA3 271 107 2.5 NM 001034023 DMAP1 106 42 2.5 NM 002434 MPG 106 42 2.5 NM 004755 RPS6KA5 106 42 2.5 NM 207121 FAM110A 180 71 2.5 NM 001080530 SNX4 375 148 2.5 NM 003755 Eif3g 676 267 2.5 NM 001130057 EEF1D 2553 1008 2.5 NM 001961 EEF2 36334 14350 2.5 NM 017806 LIME1 40 16 2.5 NM 002198 IRF1 217 86 2.5 NM 001013632 TESK2 88 35 2.5 NM 001487 BLOC1S1 136 54 2.5 NM 001199654 PMF1 273 108 2.5 NM 005189 CBX2 945 374 2.5 NM 022338 C11orf24 144 57 2.5 NM 006613 GRAP 48 19 2.5 NM 025188 TRIM5 48 19 2.5 NM 020226 PRDM8 535 212 2.5 NM 014203 AP2A1 447 177 2.5 NM 017749 AMBRA1 358 142 2.5 NR 024174 MKNK1 159 63 2.5 NM 001202545 Cux1 381 151 2.5 NM 001032281 TGFB1 603 239 2.5 NM 032304 Haghl 55 22 2.5 NM 001945 HBEGF 55 22 2.5 NM 134425 SLC2A11 55 22 2.5 NM 001199859 ANGPT1 252 one hundred 2.5 NM 001008703 C6orf1 63 25 2.5 NM 032928 TMEM159 126 fifty 2.5 NM 001997 FAU 2299 913 2.5 NM 004670 PAPSS2 70 28 2.5 NR 033329 KLHL7 78 31 2.5 NM 001008528 MXRA7 78 31 2.5 NR 033257 3 XRCC1 156 62 2.5 NM 181661 VPS13B 561 223 2.5 NR 027696 FLJ39653 4696 1867 2.5 NM 001199455 4 BRD2 171 68 2.5 NM 032932 RAB11FIP4 171 68 2.5 NM 001161565 TNRC18 1747 695 2.5 NM 015060 AVL9 178 71 2.5 NM 005108 Ydjc 462 184 2.5 NM 194460 RNF126 246 98 2.5 NM 031937 TBC1D10C 130 52 2.5 NM 001012727 AGPAT2 549 219 2.5 NM 033315 RASL10B 190 76 2.5 NM 001003690 MAD2L1BP 198 79 2.5 NM 007165 SF3A2 468 187 2.5 NM 001037328 STK11 588 235 2.5 NM 139057 ADAMTS17 22 9 2,4 NM 006066 AKR1A1 547 219 2.5 NM 000056 BCKDHB 90 36 2.5 NM 153025 C16orf55 fifteen 6 2.5

NM 001007125 C20orf201 fifteen 6 2.5 NM 032340 C6orf125 75 thirty 2.5 NM 020311 Cxcr7 fifteen 6 2.5 NM 001029955 DCAF4L1 fifteen 6 2.5 NM_001345 Dgka 52 21 2.5 NR 002605 DLEU1 37 fifteen 2.5 NM 004417 DUSP1 45 eighteen 2.5 NM 004447 EPS8 22 9 2,4 NM 000161 Gch1 52 21 2.5 NR 027028 GUSBP1 fifteen 6 2.5 NM 001145805 IRGM fifteen 6 2.5 NM_003573 LTBP4 150 60 2.5 NM 014641 5 MDC1 67 27 2.5 NR 036523 MICA 22 9 2,4 NR 039895 MIR4741 22 9 2,4 NM 017677 MTMR8 22 9 2,4 NM 001143988 NBPF6 22 9 2,4 NM 001242865 PRMT2 135 54 2.5 NM 016154 RAB4B 37 fifteen 2.5 NM 013347 RPA4 fifteen 6 2.5 NR 002981 SNORD104 52 21 2.5 NM 025250 Tubb3 thirty 12 2.5 NM 018671 UNC80 fifteen 6 2.5 NM 006295 2 VARS2 fifteen 6 2.5 NM 001007101 ZNF492 thirty 12 2.5 NM 022752 ZNF593 112 45 2.5 NM 015871 ZNF613 fifteen 6 2.5 NM 001033026 C19orf6 874 350 2.5 NM 015965 NDUFA13 574 230 2.5 NM 133374 ZNF641 259 104 2.5 NM 006377 UNC45A 711 285 2.5 NM 033086 Fgd3 207 83 2.5 NM 015649 IRF2BP1 399 160 2.5 NM 023078 Pycrl 162 65 2.5 NR 027954 Tk1 2118 850 2.5 NM 017450 BAIAP2 301 121 2.5 NM 003859 DPMI 226 91 2.5 NM 172218 SPATA13 204 82 2.5 NM 001042461 TRAPPC6A 94 38 2.5 NR 033429 ABTB1 181 73 2.5 NM 018067 MAP7D1 355 143 2.5 NM 016034 MRPS2 529 213 2.5 NM 199227 METAP1D 87 35 2.5 NM 147161 ACOT11 159 64 2.5 NM 001790 CDC25C 79 32 2.5 NM 032779 CCDC142 151 61 2.5 NM 001129819 CYB5R3 439 177 2.5 NM 015104 ATG2A 360 145 2.5 NM 024623 OGFOD2 144 58 2.5 NM 152286 PNPLA7 72 29th 2.5 NM 138352 SAMD1 144 58 2.5 NM 001001410 C16orf42 136 55 2.5 NM 004581 RABGGTA 136 55 2.5 NM 199330 HOMER2 337 136 2.5

NM 002714 PPP1R10 64 26 2.5 NM 018112 TMEM41B 64 26 2.5 NM 181842 2 ZBTB17 193 78 2.5 NM 004689 MTA1 630 254 2.5 NM 024407 NDUFS7 121 49 2.5 NM 001173408 Obsl1 300 121 2.5 NM 138414 Ccdc101 57 23 2.5 NM 004668 MGAM 57 23 2.5 NM 152222 Relt 399 161 2.5 NM 001128847 SMG6 228 92 2.5 NM 153271 SNX8 171 69 2.5 NM 013359 ZNF254 171 69 2.5 NM 001141973 ATP13A2 163 66 2.5 NM 017670 OTUB1 483 195 2.5 NM 001145815 AMDHD2 106 43 2.5 NM 030930 UNK 304 123 2.5 NM 014895 KIAA1009 49 twenty 2.5 NM 015901 NUDT13 49 twenty 2.5 NM 006287 TGFB3 99 40 2.5 NM 001100598 ZNF71 99 40 2.5 NM 024658 IP04 339 137 2.5 NM 057749 CCNE2 232 94 2.5 NM 001206951 SLC16A3 309 125 2.5 NM 001800 CDKN2D 42 17 2.5 NM 145177 DHRSX 42 17 2.5 NM 203434 IER5L 42 17 2.5 NM 016332 Sepx1 84 34 2.5 NM 031219 HDHD3 118 48 2.5 NM 020422 TMEM177 118 48 2.5 NM 001288 6 CLIC1 271 110 2.5 NM 001127365 C7orf46 76 31 2.5 NM 144606 FLCN 76 31 2.5 NR 033354 ZNF550 76 31 2.5 NM 152279 ZNF598 444 180 2.5 NM 013976 Gcdh 283 115 2.5 NR 015380 A1BG-AS1 34 fourteen 2,4 NM 013385 CYTH4 69 28 2.5 NM 002061 Gclm 103 42 2.5 NM 182647 OPRL1 103 42 2.5 NM 016580 PCDH12 34 fourteen 2,4 NM 214710 PRSS57 1242 504 2.5 NM 001031618 SPDYE3 34 fourteen 2,4 NM 001199662 PMF1-BGLAP 130 53 2.5 NM 002162 ICAM3 733 298 2.5 NM 021805 Sigirr 157 64 2.5 NM 014855 KIAA0415 342 139 2.5 NM 001165877 ATP5L2 61 25 2,4 NM 020664 Decr2 123 fifty 2.5 NM 014046 4 MRPS18B 61 25 2,4 NM 003482 Mll2 3522 1432 2.5 NM 004445 EPHB6 204 83 2.5 NM 000992 Rpl29 7063 2874 2.5 NM 173545 Apl 27 eleven 2.5 NM 001003398 BICD1 27 eleven 2.5

NM 001135751 DERL3 27 eleven 2.5 NM 022760 FAM113A 297 121 2.5 NR 003579 FRG1B 135 55 2.5 NM 001190825 MAFIP 27 eleven 2.5 NM 002412 MGMT 108 44 2.5 NM 020654 Senp7 162 66 2.5 NM 016072 GOLT1B 235 96 2,4 NM 001032383 PQBP1 208 85 2,4 NM 006014 LAGE3 127 52 2,4 NM 001136191 Kank2 328 134 2,4 NM 015953 Nosip 174 71 2.5 NM 001319 CSNK1G2 669 273 2.5 NM 004838 Homer3 220 90 2,4 NM 054035 UNC13B 73 thirty 2,4 NM 001029891 PGAM4 139 57 2,4 NM 005892 FMNL1 942 385 2,4 NM 152482 C19orf25 205 84 2,4 NM 000263 NAGLU 283 116 2,4 NM 007209 Rpl35 1309 536 2,4 NM 152431 PIWIL4 85 35 2,4 NM 175872 ZNF804A 190 78 2,4 NR 038257 TRPM4 432 177 2,4 NM 001166664 CD244 163 67 2,4 NM 001773 Cd34 1857 761 2,4 NM 138353 DCAF15 358 147 2,4 NM 001040031 Cd37 495 203 2,4 NM 152426 APOBEC3D 39 16 2,4 NR 026997 C22orf34 78 32 2,4 NM 001168648 CNKSR2 39 16 2,4 NM 016144 COMMD10 39 16 2,4 NM 152511 DUSP18 19 8 2,4 NM 000173 GP1BA 39 16 2,4 NM 001003795 GTF2IRD2B 39 16 2,4 NR 003675 Gusbp5 117 48 2,4 NM 013320 HCFC2 78 32 2,4 NM 018012 KIF26B 19 8 2,4 NR 036154 MIR3187 58 24 2,4 NM 018728 MY05C 19 8 2,4 NM 177533 NUDT14 78 32 2,4 NM 017893 SEMA4G 19 8 2,4 NM 001122752 SERPINI1 19 8 2,4 NM 003114 SPATC1 19 8 2,4 NM 052874 STX8 19 8 2,4 NM 018252 TMEM22 19 8 2,4 NM 001136044 TMX2-CTNND1 97 40 2,4 NM 173555 TYW1B 58 24 2,4 NM 001142864 Fam38a 2185 897 2,4 NM 016129 COPS4 168 69 2,4 NM 080627 KIAA0889 168 69 2,4 NM 207005 USP19 762 313 2,4 NM 031917 ANGPTL6 219 90 2,4 NM 020442 Vasp 328 135 2,4 NM 001184751 ZNF41 109 45 2,4 NM 003310 Tst 180 74 2,4

NM 001022 RPS19 3846 1582 2,4 NM 001862 COX5B 493 203 2,4 NM 001136180 HSBP1L1 70 29th 2,4 NM 175744 RHOC 282 116 2,4 NM 015994 ATP6V1D 121 fifty 2,4 NM_016337 EVL 121 fifty 2,4 NM 001134430 Tox 243 one hundred 2,4 NM 144716 Ccdc12 102 42 2,4 NM 001129885 Cpsf4l 51 21 2,4 NM 001144958 EFCAB4B 102 42 2,4 NM 016093 RPL26L1 51 21 2,4 NR 002450 SNRNP25 133 55 2,4 NM 001039841 ARHGAP11B 82 34 2,4 NM 001105515 ABCC4 196 81 2,4 NM 017857 ST20-MTHFS 114 47 2,4 NM 003036 SKI 861 355 2,4 NM 002168 IDH2 1494 616 2,4 NM 001002796 MCTP1 145 60 2,4 NM 015125 Cic 354 146 2,4 NM 145253 Fam100a 63 26 2,4 NM 001144891 MBIP 63 26 2,4 NM 015178 RHOBTB2 94 39 2,4 NM 020175 DUS3L 363 150 2,4 NM 006455 LEPREL4 256 106 2,4 NM 001127257 SLC39A14 280 116 2,4 NM 024419 PGS1 585 242 2,4 NM 032208 ANTXR1 217 90 2,4 NM 001178 Arntl 43 eighteen 2,4 NR 028324 FLJ45445 43 eighteen 2,4 NM 004127 GPS1 957 396 2,4 NR 024053 1 HCG18 43 eighteen 2,4 NM 001201573 NUBPL 43 eighteen 2,4 NR 034108 TRAIP 174 72 2,4 NM 032799 ZDHHC8 130 54 2,4 NM 003575 Znf3 174 72 2,4 NM 004604 STXBP2 360 149 2,4 NM 001163922 Wbp1 229 95 2,4 NM 001145636 C1orf228 922 382 2,4 NM 177980 CDH26 99 41 2,4 NM 207368 FAM195B 198 82 2,4 NM 001171 ABCC6 55 23 2,4 NM 006869 ADAP1 111 46 2,4 NM 006411 1 AGPAT1 55 23 2,4 NM 024097 C1orf50 55 23 2,4 NM 014648 Dzip3 111 46 2,4 NM 025241 UBXN8 55 23 2,4 NM 001696 ATP6V1E1 658 273 2,4 NM 012290 TMED8 67 28 2,4 NM 001040110 NRF1 159 66 2,4 NM 005694 COX17 91 38 2,4 NM 001160172 NAT1 91 38 2,4 NM 001018 RPS15 1273 529 2,4 NM 025209 Epc1 310 129 2,4 NM 001030001 RPS29 681 283 2,4

NM 002018 Flu 813 338 2,4 NM 006639 CYSLTR1 163 68 2,4 NM 004579 MAP4K2 163 68 2,4 NM 022727 TRMT61A 175 73 2,4 NM 058182 FAM165B 187 78 2,4 NM 006026 H1fx 187 78 2,4 NM 001190991 CNPY2 199 83 2,4 NM 003670 BHLHE40 223 93 2,4 NM 012292 HMHA1 1194 497 2,4 NM 014711 Ccp110 319 133 2,4 NM 194326 RPS19BP1 331 138 2,4 NM 024669 ANKRD55 12 5 2,4 NM 001039083 ARL17B 24 10 2,4 NM 001670 ARVCF 36 fifteen 2,4 NM 006994 BTN3A3 60 25 2,4 NM 001010863 C10orf128 12 5 2,4 NM 018186 C1orf112 60 25 2,4 NM 206921 C6orf204 12 5 2,4 NM 004196 CDKL1 24 10 2,4 NM 182853 CREM 36 fifteen 2,4 NM 001249 ENTPD5 48 twenty 2,4 NM 001142800 Eys 12 5 2,4 NM 001122834 H hat 36 fifteen 2,4 NM 001243195 INPP5F 12 5 2,4 NM 000214 Jag1 12 5 2,4 NM 005560 LAMA5 36 fifteen 2,4 NM 024316 LENG1 72 thirty 2,4 NM 001130090 LRRC48 12 5 2,4 NM 003010 MAP2K4 132 55 2,4 NM 014975 MAST1 12 5 2,4 NM 025176 Ninl 120 fifty 2,4 NM 014323 PATZ1 540 225 2,4 NR 027694 PP2D1 48 twenty 2,4 NM 033215 PPP1R3F 12 5 2,4 NM 024070 PVRIG 12 5 2,4 NM 020436 SALL4 24 10 2,4 NM 017789 SEMA4C 36 fifteen 2,4 NM 001024939 SLC2A9 24 10 2,4 NM 032354 TMEM134 12 5 2,4 NM 018056 TMEM42 120 fifty 2,4 NM 001037330 TRIM23 36 fifteen 2,4 NM 001039111 TRIM9 24 10 2,4 NM 138466 ZNF846 24 10 2,4 NM 002229 Jung 457 191 2,4 NM 001006947 UNC119 136 57 2,4 NM 001013699 H3F3C 795 332 2,4 NM 012398 PIP5K1C 261 109 2,4 NM 001145023 SCRN2 249 104 2,4 NM 001130029 RELL2 237 99 2,4 NM 145647 Wdr7 165 69 2,4 NM 001134433 AZI2 282 118 2,4 NM 001032364 Ggt1 64 27 2,4 NM 174940 TMEM86B 64 27 2,4 NM 002254 KIF3C 246 103 2,4

NM 002841 PTPRG 298 125 2,4 NM 013379 DPP7 948 397 2,4 NM 178495 ITPRIPL1 117 49 2,4 NM 001031723 DNAJB14 169 71 2,4 NM 006742 PSKH1 339 142 2,4 NM 001033551 TOMM40 339 142 2,4 NM 001421 ELF4 823 345 2,4 NM 006012 CLPP 262 110 2,4 NM 002126 Hlf 52 22 2,4 NM 139032 MAPK7 52 22 2,4 NM 052818 N4BP2L1 52 22 2,4 NM 133373 PLCD3 52 22 2,4 NR 027861 1 ZNF660 105 44 2,4 NM 145245 EVI5L 145 61 2,4 NM 016368 ISYNA1 145 61 2,4 NR 036556 ANKRD54 133 56 2,4 NM 004900 APOBEC3B 81 34 2,4 NM 178830 C19orf47 81 34 2,4 NM 001042493 C6orf162 81 34 2,4 NM 006668 CYP46A1 40 17 2,4 NM 020116 Fstl5 40 17 2,4 NM 177417 KLC3 40 17 2,4 NM 144593 Rhebl1 40 17 2,4 NM 001042631 SDHAF1 121 51 2,4 NM 030899 Znf34 40 17 2,4 NM 000177 GSN 2115 888 2,4 NM 001002877 Thra 300 126 2,4 NM 014297 ETHE1 109 46 2,4 NM 001100599 ZNF710 397 167 2,4 NM 004329 BMPR1A 178 75 2,4 NM 033358 Casp8 138 58 2,4 NM 005144 HR 138 58 2,4 NM 017514 PLXNA3 69 29th 2,4 NM 001031628 SMAP1 166 70 2,4 NM 001029882 AHDC1 126 53 2,4 NM 016108 Aig1 126 53 2,4 NM 001001795 C8orf82 211 89 2,4 NR 002595 RPL31P11 354 149 2,4 NR 027995 ANKRD20A9P 57 24 2,4 NM 206895 C2orf82 28 12 2,3 NM 144611 CYB5D2 85 36 2,4 NM 001134945 Ghrl 28 12 2,3 NM 153832 Gpr161 28 12 2,3 NM 001166292 PTCH2 28 12 2,3 NM 170774 RASSF2 57 24 2,4 NM 033070 CECR5 550 232 2,4 NM 000956 PTGER2 130 55 2,4 NR 026660 RPL19P12 946 399 2,4 NM 001201472 COR07 204 86 2,4 NM 006039 MRC2 4384 1849 2,4 NM 018190 Bbs7 73 31 2,4 NM 001130917 Lilra2 808 341 2,4 NM 020461 TXNDC17 73 31 2,4 NM 014171 CRIPT 118 fifty 2,4

NM 006284 TAF5 118 fifty 2,4 NM 005273 GNB2 756 319 2,4 NM 031287 SF3B5 417 176 2,4 NM 207174 ABCG1 45 19 2,4 NM 013375 ABT1 225 95 2,4 NR 024573 Ghdc 135 57 2,4 NM 001142450 SPNS3 45 19 2,4 NR 015370 NCRNA00219 511 216 2,4 NM 030628 INTS5 331 140 2,4 NM 006257 PRKCQ 151 64 2,4 NM 033428 C9orf123 106 45 2,4 NM 001003897 MANBAL 106 45 2,4 NM 005645 TAGAP 213 90 2,4 NM 080626 BRI3BP 274 116 2,4 NM 052834 WDTC1 459 194 2,4 NM 001242476 ZNF362 229 97 2,4 NM 014343 CLDN15 61 26 2,3 NM 001190844 TMEM38B 295 125 2,4 NM 001144013 RGPD3 156 66 2,4 NM 018364 RSBN1 312 132 2,4 NM 001146154 PTGR2 94 40 2,4 NM 022566 MESDC1 205 87 2,4 NM 203352 PDLIM7 111 47 2,4 NM 002801 PSMB10 144 61 2,4 NM 001030 RPS27 144 61 2,4 NM 001001928 PPARA 193 82 2,4 NM 007346 Ogfr 424 180 2,4 NM 020179 C11orf75 33 fourteen 2,4 NM 001195125 C16orf95 16 7 2,3 NM 001145252 CFP 99 42 2,4 NM 001202558 CHURC1-FNTB 198 84 2,4 NM 014780 Cul7 181 77 2,4 NM 018351 Fgd6 16 7 2,3 NM 002203 ITGA2 16 7 2,3 NM 032534 KRBA1 214 91 2,4 NM 024507 KREMEN2 16 7 2,3 NM 006337 MCRS1 330 140 2,4 NM 182980 OSGIN1 16 7 2,3 NM 001243136 Peli3 49 21 2,3 NM 001128602 RASGRP1 33 fourteen 2,4 NR 037612 SEPT5-GP1BB 412 175 2,4 NM 001164771 SLC7A5P2 16 7 2,3 NR 002594 SLC9A7P1 16 7 2,3 NM 020705 TBC1D3P1-DHX40P1 16 7 2,3 NM 006510 3 TRIM3 16 7 2,3 NM 001008485 SLC41A3 235 one hundred 2,4 NM 006640 Sept9 1521 646 2,4 NM 001077624 ZNF865 186 79 2,4 NM 182687 PKMYT1 273 116 2,4 NM 021144 PSIP1 360 153 2,4 NM 001202403 ASB6 327 139 2,4 NM 001110556 Flna 2794 1188 2,4 NM 001093772 Kit 7143 3110 2,3 NM 024050 Dda1 244 104 2,3

NM 001004356 FGFRL1 70 thirty 2,3 NM 000213 ITGB4 70 thirty 2,3 NM 032876 Jub 70 thirty 2,3 NM 024109 METTL22 70 thirty 2,3 NM 001097604 3 XK 759 323 2,3 NM 201453 1 CBWD3 108 46 2,3 NM 006494 Erf 561 239 2,3 NM 002117 2 Hla-c 237 101 2,3 NM 012072 Cd93 91 39 2,3 NR 027634 Fam35b2 91 39 2,3 NM 020924 ZBTB38 91 39 2,3 NM 014729 TP53I3 166 71 2,3 NM 001165 BIRC3 37 16 2,3 NM 024713 C15orf29 37 16 2,3 NM 018948 ERRFI1 37 16 2,3 NM 007270 FKBP9 37 16 2,3 NM 031485 GRWD1 375 160 2,3 NR 024448 GUSBP11 75 32 2,3 NM 015202 KIAA0556 37 16 2,3 NM 016199 LSM7 337 144 2,3 NM 004546 NDUFB2 562 240 2,3 NM 032349 NUDT16L1 225 96 2,3 NM 001080419 USE1 225 96 2,3 NM 018260 ZNF707 112 48 2,3 NM 015374 Surf1 246 105 2,3 NM 030928 CDT1 1192 509 2,3 NM 001166167 NEK6 342 146 2,3 NM 006598 SLC12A7 304 130 2,3 NM 014061 MAGEH1 96 41 2,3 NM 014045 Lrp10 501 214 2,3 NM 032036 IFI27L2 154 66 2,3 NM 021228 SCAF1 463 198 2,3 NM 018667 SNAP47 213 91 2,3 NM 198476 C19orf54 117 fifty 2,3 NM 018079 SRGAP2P2 58 25 2,3 NM 006369 LRRC41 493 211 2,3 NM 004085 Tipin 159 68 2,3 NM 181597 USF1 238 102 2,3 NM 018641 CHST12 180 77 2,3 NM 022730 COPS7B 280 120 2,3 NM 004704 RRP9 264 113 2,3 NM 001021 Rps17 2503 1072 2,3 NM 001199057 1 RPS17L 2503 1072 2,3 NM 001031716 OBFC2A 205 88 2,3 NM 001199119 TRIM41 289 124 2,3 NM 001085461 CTNND1 63 27 2,3 NR 027468 1 FLJ39739 42 eighteen 2,3 NM 001144869 LIPT2 21 9 2,3 NR 024402 MGC23284 21 9 2,3 NR 033882 MLK7-AS1 21 9 2,3 NM 002513 NME3 63 27 2,3 NM 001077497 6 PRR3 21 9 2,3 NR 037945 STX4 63 27 2,3 NM 152493 ZNF408 126 54 2,3

NM 001142577 ZNF784 84 36 2,3 NM 013321 Solh 256 110 2,3 NM 004225 Mfhas1 450 193 2,3 NM 198526 ZNF737 193 83 2,3 NM 002908 Rel 151 65 2,3 NM 022483 C5orf28 328 141 2,3 NM 052813 CARD9 109 47 2,3 NM 001164440 ANKRD33B 333 143 2,3 NM 004807 HS6ST1 312 134 2,3 NM 005148 UNC13D 1003 431 2,3 NM 173680 ZNF780A 67 29th 2,3 NM 178129 1 P2RY8 949 408 2,3 NM 203282 ZNF282 591 254 2,3 NM 004418 DUSP2 114 49 2,3 NM 003655 CBX4 937 403 2,3 NM 000117 EMD 367 158 2,3 NM 016404 TRMT2A 507 218 2,3 NM 001347 DGKQ 186 80 2,3 NM 007207 DUSP10 46 twenty 2,3 NM 002428 MMP15 46 twenty 2,3 NM 145018 C11orf82 165 71 2,3 NM 000714 TSSC1 381 164 2,3 NM 032636 PSRC1 216 93 2,3 NM 213725 RPLP1 3439 1481 2,3 NM 001195215 DENND1B 220 95 2,3 NM 014606 Herc3 220 95 2,3 NM 020761 RPTOR 763 329 2,3 NM 144994 ANKRD23 51 22 2,3 NM 001199055 C16orf5 331 143 2,3 NM 017766 Casz1 76 33 2,3 NM 001195422 Gtpbp3 255 110 2,3 NM 001566 INPP4A 76 33 2,3 NM 175061 Jazf1 25 eleven 2,3 NM 033343 Lhx4 25 eleven 2,3 NM 021177 1 LSM2 25 eleven 2,3 NM 016734 PAX5 51 22 2,3 NM 015324 RRP8 204 88 2,3 NM 006247 PPP5C 361 156 2,3 NM 003091 SNX29 157 68 2,3 NM 020971 SQSTM1 1422 614 2,3 NM 007371 BRD3 796 344 2,3 NM 015263 DMXL2 213 92 2,3 NM 001039132 ICAM4 106 46 2,3 NM 016558 SCAND1 213 92 2,3 NM 001334 Ctso 81 35 2,3 NM 018314 Ugdh 81 35 2,3 NM 001166621 TRAPPC5 298 129 2,3 NM 005197 Foxn3 1911 826 2,3 NM 001204451 Ccpg1 465 201 2,3 NM 005229 ELK1 465 201 2,3 NM 024329 Efhd2 333 144 2,3 NM 021012 KCNJ12 55 24 2,3 NM 030583 MATN2 111 48 2,3 NM 002833 PTPN9 448 194 2,3

NM 020428 SLC46A1 85 37 2,3 NM 001031721 ZNF618 256 111 2,3 NM 006397 RNASEH2A 372 161 2,3 NM 001134368 SLC7A2 201 87 2,3 NM 030813 CLPB 231 one hundred 2,3 NM 021724 NR1D1 115 fifty 2,3 NM 017907 Lamtor1 376 163 2,3 NM 133638 ADAMTS19 thirty 13 2,3 NR 027398 Gfod2 thirty 13 2,3 NM 001040616 LINS 60 26 2,3 NM 001178102 LOX thirty 13 2,3 NM 032348 MXRA8 thirty 13 2,3 NM 153449 SLC30A1 120 52 2,3 NM 007237 SPAG1 thirty 13 2,3 NM 032737 LMNB2 1806 783 2,3 NM 001008395 C7orf59 214 93 2,3 NM 024757 EHMT1 678 294 2,3 NR 033338 C17orf70 438 190 2,3 NM 015949 Get4 283 123 2,3 NM 018216 PANK4 94 41 2,3 NM 001145909 TAOK2 189 82 2,3 NR 027882 ΒΑΧ 159 69 2,3 NM 014315 KLHDC2 159 69 2,3 NM 021825 CCDC90B 193 84 2,3 NM 001137551 LRRFIP1 129 56 2,3 NM 001184746 PAFAH1B2 64 28 2,3 NM 020895 GRAMD1A 357 155 2,3 NM 001014763 ETFB 228 99 2,3 NR 027179 NCRNA00188 654 284 2,3 NM 001024809 Rara 133 58 2,3 NM 021194 SLC38A10 1495 650 2,3 NM 001001852 PIM3 409 178 2,3 NM 001009 Rps5 3457 1503 2,3 NM 001135919 SLC4A2 720 313 2,3 NM 001193329 C9orf3 34 fifteen 2,3 NM 001193466 KIAA1267 379 165 2,3 NR 039918 MIR4761 34 fifteen 2,3 NM 004536 NAIP 34 fifteen 2,3 NM 020144 Papolb 34 fifteen 2,3 NM 138374 ZNF862 172 75 2,3 NM 001172566 MYD88 427 186 2,3 NR 024603 MPDU1 250 109 2,3 NM 018174 MAPI S 432 188 2,3 NM 022047 Def6 579 252 2,3 NM 004563 PCK2 622 271 2,3 NM 001040118 ARAP1 882 384 2,3 NM 004701 CCNB2 441 192 2,3 NM 000734 CD247 73 32 2,3 NM 004448 ERBB2 73 32 2,3 NM 001034845 GALNTL6 73 32 2,3 NM 006545 NPRL2 186 81 2,3 NM 199249 C19orf48 1198 522 2,3 NM 000991 Rpl28 3604 1570 2,3 NM 001008388 CISD2 268 117 2,3

NR 002796 AKR7A2P1 39 17 2,3 NM 001124767 C3orf78 39 17 2,3 NM 133328 DEDD2 78 34 2,3 NR 003260 DNM1P46 39 17 2,3 NM 022664 ECM1 195 85 2,3 NM 031904 FRMD8 390 170 2,3 NM 002269 Kpna5 39 17 2,3 NR 026052 MGC2752 156 68 2,3 NM 198513 PHF20L1 199 87 2,3 NM 001946 DUSP6 646 282 2,3 NM 001130410 ACAA1 243 106 2,3 NM 198402 PTPLB 165 72 2,3 NM 001099456 NPW 291 127 2,3 NM 006268 DPF2 543 237 2,3 NM 005194 CEBPB 304 133 2,3 NM 018390 PLCXD1 130 57 2,3 NM 015241 MICAL3 400 175 2,3 NM 006923 SDF2 270 118 2,3 NM 181050 Axin1 274 120 2,3 NM 022153 C10orf54 91 40 2,3 NM 001193375 NDUFA11 231 101 2,3 NM 001199865 KIF16B 139 61 2,3 NM 001184898 PHF8 187 82 2,3 NM 020421 ADCK1 48 21 2,3 NM 005165 ALDOC 48 21 2,3 NM 207315 CMPK2 96 42 2,3 NM 012401 PLXNB2 96 42 2,3 NM 001242534 MFSD11 244 107 2,3 NR 033344 SLC39A10 297 130 2,3 NM 001013 Rps9 3367 1474 2,3 NM 006739 Mcm5 1455 637 2,3 NM 022719 DGCR14 one hundred 44 2,3 NM 024641 MANEA one hundred 44 2,3 NM 001171946 SUN2 1612 706 2,3 NM 181532 ERAS 253 111 2,3 NM 006404 PROCR 406 178 2,3 NM 000485 APRT 511 224 2,3 NM 001173988 C9orf86 1132 496 2,3 NM 015957 APIP 157 69 2,3 NM 001196 BID 630 276 2,3 NM 001217 CA11 52 23 2,3 NM 001810 Cenpb 1156 507 2,3 NM 014047 C19orf53 333 146 2,3 NM 021210 TRAPPC4 223 98 2,3 NM 020801 ARRDC3 280 123 2,3 NR 003558 Wdr18 337 148 2,3 NM 020914 RNF213 2818 1236 2,3 NM 001242821 Def8 57 25 2,3 NM 020899 ZBTB49 114 fifty 2,3 NM 032789 PARP10 522 229 2,3 NR 028451 BCAT2 175 77 2,3 NM 198401 ANKRD46 118 52 2,3 NM 080655 C9orf30 298 131 2,3 NM 014960 ARSG 61 27 2,3

NR 030770 CCM2 307 135 2,3 NM 173502 PRSS36 61 27 2,3 NM 001100814 TMEM62 123 54 2,3 NM_023072 Zxdc 246 108 2,3 NM 001002247 ANAPC11 576 253 2,3 NM 001199254 SGOL1 66 29th 2,3 NM 001010927 TIMM13 396 174 2,3 NM 001207009 ZNF543 66 29th 2,3 NM_001079804 GAA 1342 590 2,3 NM 001031680 RUNX3 339 149 2,3 NM 001017928 Ccdc58 136 60 2,3 NM 024989 PGAP1 136 60 2,3 NM 032901 C12orf62 343 151 2,3 NM 031280 MRPS15 207 91 2,3 NM 005735 ACTR1B 484 213 2,3 NM 080605 B3GALT6 423 186 2,3 NM 001381 DOK1 1128 496 2,3 NM 020205 OTUD7B 70 31 2,3 NM 001130160 STUB1 366 161 2,3 NM 130468 CHST14 75 33 2,3 NM 001113347 ECE1 234 103 2,3 NM 032522 ZBTB4 313 138 2,3 NM 138384 MTG1 79 35 2,3 NM 015148 PASK 159 70 2,3 NM 012268 PLD3 495 218 2,3 NM 001110354 ZSCAN2 247 109 2,3 NM 020862 LRFN1 88 39 2,3 NM 021991 Jup 1075 474 2,3 NM 001203262 NDUFC2-KCTD14 363 160 2,3 NR 034109 TRAF7 456 201 2,3 NM 015909 NBAS 465 205 2,3 NM 172389 NFATC1 186 82 2,3 NM 001143994 RASSF7 93 41 2,3 NM 080704 TSC22D4 664 293 2,3 NM 001143905 C12orf65 190 84 2,3 NM 001243283 Alcam 106 47 2,3 NM 002972 SBF1 1096 484 2,3 NM 015159 FAM168A 111 49 2,3 NM 001136498 CISD3 337 149 2,3 NM 001145396 ALDH16A1 226 one hundred 2,3 NM 212554 METTL10 115 51 2,3 NM 001003828 PARVB 267 118 2,3 NM 022839 MRPS11 276 122 2,3 NM 024036 LRFN4 147 65 2,3 NM 033257 DGCR6L 165 73 2,3 NM 006532 Ell 330 146 2,3 NR 033339 C4orf42 169 75 2,3 NM 003595 TRAF2 169 75 2,3 NM 033110 A2LD1 178 79 2,3 NM 173082 SHPRH 178 79 2,3 NM 001376 DYNC1H1 2713 1201 2,3 NM 001080542 Fbf1 196 87 2,3 NM 004638 1 PRRC2A 397 176 2,3 NM 002360 MAFK 237 105 2,3

NM 033452 TRIM68 246 109 2,3 NM 144679 C17orf56 250 111 2,3 NM 019037 EXOSC4 268 119 2,3 NM 001130072 EPN1 349 155 2,3 NM 213601 TMEM104 453 201 2,3 NM 182565 Fam100b 942 418 2,3 NM 015594 TBCD 529 235 2,3 NM 021940 SMARCA4 925 411 2,3 NM 001142653 AARSD1 9 four 2,3 NM 032592 ACCS 63 28 2,3 NM 032955 5 Aif1 27 12 2,3 NM 170726 ALDH4A1 49 22 2.2 NM 001164443 ANKRD31 9 four 2,3 NM 019087 ARL15 27 12 2,3 NM 018179 ATF7IP 400 178 2.2 NM 178326 ATG4B 108 48 2,3 NM 001164782 ATXN3 225 one hundred 2,3 NM 001136537 BTBD19 9 four 2,3 NR 015404 C12orf47 175 78 2.2 NM 001031726 C19orf12 112 fifty 2.2 NM 001135580 C19orf71 27 12 2,3 NR 027790 C21orf34 9 four 2,3 NM 182597 C7orf53 13 6 2.2 NR 023390 C9orf130 9 four 2,3 NM 032310 C9orf89 27 12 2,3 NM 001218 CA12 13 6 2.2 NM 024565 CCNJL 49 22 2.2 NM 174892 CD300LB 9 four 2,3 NM 004364 NORTH 220 98 2.2 NM 054113 Cib3 eighteen 8 2,3 NM 001031617 COX19 337 150 2.2 NM 001348 DAPK3 198 88 2,3 NM 198083 DHRS4L2 99 44 2,3 NR 023383 DTX2P1-UPK3BP1-PMS2P11 eighteen 8 2,3 NM 004444 EPHB4 85 38 2.2 NM 005236 ERCC4 67 thirty 2.2 NM 033266 ERN2 13 6 2.2 NM 016046 EXOSC1 45 twenty 2,3 NM 016049 FAM158A 40 eighteen 2.2 NM 207334 Fam43b 9 four 2,3 NR 024254 FAM86FP eighteen 8 2,3 NM 001080472 Fitm2 13 6 2.2 NM 001145777 FKBP5 229 102 2.2 NR 037596 FLJ14186 27 12 2,3 NR 033839 FLJ39051 9 four 2,3 NM 000148 FUT1 22 10 2.2 NM 003505 Fzd1 31 fourteen 2.2 NM 024637 GAL3ST4 9 four 2,3 NM 006143 Gpr19 9 four 2,3 NM 001080423 GRIP2 9 four 2,3 NR 002932 GSTM2P1 63 28 2,3 NM 173497 HECTD2 27 12 2,3 NM 001114380 ITGAL 9 four 2,3

NM 014505 KCNMB4 13 6 2.2 NM 001100915 KCTD19 9 four 2,3 NM 001170331 LANCL3 13 6 2.2 NM__001014989 Lat 9 four 2,3 NM 153486 LDHD 13 6 2.2 NM 012163 LRRC29 9 four 2,3 NM 001146082 MBOAT7 193 86 2.2 NM 015078 Mcf2l2 9 four 2,3 NR 031589 MIR1178 9 four 2,3 NM 001040000 MLLT4 247 110 2.2 NM 019556 MOSPD1 90 40 2,3 NM 030811 MRPS26 306 136 2,3 NM 000265 NCF1 9 four 2,3 NR 040116 NSF 85 38 2.2 NM 007224 NXPH4 9 four 2,3 NM 030758 OSBP2 9 four 2,3 NM 007365 Padi2 139 62 2.2 NM 001037281 PARD6A 54 24 2,3 NM 001111307 PDE4A 130 58 2.2 NM 005391 PDK3 22 10 2.2 NM 152305 POGLUT1 445 198 2.2 NM 000941 Por 139 62 2.2 NM 020440 PTGFRN 9 four 2,3 NM 002830 PTPN4 27 12 2,3 NM 206827 RASL11A 9 four 2,3 NM 001195303 Rgs5 9 four 2,3 NM 173557 RNF152 9 four 2,3 NM 170769 Rnf39 9 four 2,3 NM 015077 Sarm1 63 28 2,3 NM 014654 Sdc3 9 four 2,3 NM 004186 SEMA3F 49 22 2.2 NM 015559 SETBP1 13 6 2.2 NM 001001713 SH3BGR 27 12 2,3 NM 031469 SH3BGRL2 9 four 2,3 NM 001177549 SIGLEC6 31 fourteen 2.2 NM 001242366 SLC46A3 45 twenty 2,3 NM 001128848 SMCR7 45 twenty 2,3 NR 024543 SNN 40 eighteen 2.2 NR 024542 SNORA32 9 four 2,3 NR 003078 SNORD35A 9 four 2,3 NM 005632 SP140 9 four 2,3 NM 001146316 SPTBN1 2479 1102 2.2 NM 003765 STX1B 9 four 2,3 NM 001165030 TMEM53 9 four 2,3 NM 024956 TMEM63C 27 12 2,3 NR 026678 TMEM80 171 76 2,3 NM 145274 TMUB1 175 78 2.2 NM 004412 TRIM16 31 fourteen 2.2 NM 020972 ZGLP1 27 12 2,3 NM 001029976 ZNF165 eighteen 8 2,3 NM 003801 GPAA1 784 349 2.2 NM 001006623 Wdr33 532 237 2.2 NM 006456 ST8SIA4 348 155 2.2 NM 015894 STRA13 348 155 2.2

NM 001242395 Tac01 330 147 2.2 NM 030577 TMEM206 294 131 2.2 NM 207585 IFNAR2 262 117 2.2 NM 022151 MOAP1 249 111 2.2 NM 212535 PRKCB 249 111 2.2 NM 022061 MRPL17 471 210 2.2 NM 021818 SAV1 235 105 2.2 NM 080656 CDKN2AIPNL 231 103 2.2 NM 005631 SMYD2 208 93 2.2 NM 002975 CLEC11A 2970 1325 2.2 NM 030812 ACTL8 535 239 2.2 NM 199054 MKNK2 703 314 2.2 NM 024762 ZNF574 331 148 2.2 NM 001142557 Hmmr 327 146 2.2 NM 000973 RPL8 7986 3566 2.2 NM 014595 NT5C 313 140 2.2 NM 006435 IFITM2 468 209 2.2 NM 030767 AKNA 618 276 2.2 NM 001166139 LCORL 145 65 2.2 NM 030935 TSKU 145 65 2.2 NM 032595 PPP1R9B 1879 840 2.2 NM 139280 ORMDL3 523 234 2.2 NM 015255 UBXN6 364 163 2.2 NM 001130978 Dysf 241 108 2.2 NM 152592 C14orf49 118 53 2.2 NM 001172666 RAB40C 118 53 2.2 NM 138769 Rhot2 237 106 2.2 NM 198088 ZNF253 114 51 2.2 NM 032608 MY018B 219 98 2.2 NM 016373 XAGE1A 109 49 2.2 NM 001097592 3 XAGE1B 109 49 2.2 NM 001097596 3 XAGE1C 109 49 2.2 NM 001097597 3 XAGE1D 109 49 2.2 NM 020411 3 XAGE1E 109 49 2.2 NR 033953 P2RX7 214 96 2.2 NR 003068 SNRNP70 534 239 2.2 NM 001099627 Fam54b 205 92 2.2 NM 014216 ITPK1 511 229 2.2 NM 025029 MZT2B 306 137 2.2 NM 012086 GTF3C3 507 227 2.2 NM 032790 Oram 196 88 2.2 NM 001098614 PUS7L 196 88 2.2 NR 002924 TBKBP1 585 262 2.2 NM 022337 RAB38 96 43 2.2 NM 012088 PGLS 187 84 2.2 NM 001784 Cd97 462 207 2.2 NM 001024382 Hmbs 183 82 2.2 NM 001113755 TYSND1 357 160 2.2 NM 020725 ATXN7L1 265 119 2.2 NR 004847 1 UBL7 169 76 2.2 NM 015254 KIF13B 252 113 2.2 NM 004381 2 ATF6B 82 37 2.2 NM 001163023 PLK1S1 325 146 2.2 NM 004957 FPGS 568 255 2.2

NM 003325 Hira 486 218 2.2 NM 033240 PML 642 288 2.2 NM 000681 ADRA2A 78 35 2.2 NM 198531 ATP9B 156 70 2.2 NM 015192 PLCB1 78 35 2.2 NR 003661 UBE2S 303 136 2.2 NR 026890 RPSAP9 666 299 2.2 NM 006133 DAGLA 147 66 2.2 NM 022117 TSSC4 294 132 2.2 NM 001172659 ZFYVE9 73 33 2.2 NM 001002836 ZNF799 73 33 2.2 NM 005993 TBX1 285 128 2.2 NM 001134431 TP53I13 285 128 2.2 NM 018218 UXS1 211 95 2.2 NR 026844 ANKRD36BP1 69 31 2.2 NM 031910 C1QTNF6 69 31 2.2 NM 014571 Heyl 138 62 2.2 NR 033688 Pef1 207 93 2.2 NM 006747 Sipa1 474 213 2.2 NM 018166 FAM176B 64 29th 2.2 NM 183421 FBX025 64 29th 2.2 NM 005537 Ing1 129 58 2.2 NM 001164811 PET117 253 114 2.2 NM 198264 FAM189B 189 85 2.2 NM 002390 ADAM 11 60 27 2.2 NM 019007 ARMCX6 180 81 2.2 NM 007098 CLTCL1 60 27 2.2 NM 002757 MAP2K5 60 27 2.2 NM 003046 SLC9A7 60 27 2.2 NM 013241 FHOD1 402 181 2.2 NM 030783 PTDSS2 171 77 2.2 NM 130847 AMOTL1 508 229 2.2 NM 013291 Cpsf1 1498 675 2.2 NM 138418 FAM195A 55 25 2.2 NM 023007 Jmjd4 222 one hundred 2.2 NM 182958 KAT8 111 fifty 2.2 NM 003730 RNASET2 495 223 2.2 NM 182526 TMEM39B 162 73 2.2 NM 016567 Bccip 213 96 2.2 NR 037849 INO80B-WBP1 315 142 2.2 NM 001014432 AKT1 727 328 2.2 NM 000465 BARD1 102 46 2.2 NM 001001520 HDGFRP2 306 138 2.2 NM 014714 IFT140 51 23 2.2 NM 198970 AES 705 318 2.2 NM 015292 ESYT1 1494 674 2.2 NM 016219 MAN1B1 747 337 2.2 NM 153690 Fam43a 148 67 2.2 NM 032718 MFSD9 97 44 2.2 NM 001130677 C17orf96 241 109 2.2 NM 001105247 ARMC5 144 65 2.2 NM 001099271 Poc5 144 65 2.2 NM 004422 DVL2 190 86 2.2 NM 001199196 ARMC6 664 300 2.2

NM 000980 RPL18A 1599 722 2.2 NM 144581 C14orf149 93 42 2.2 NR 002786 Cidecp 46 21 2.2 NM 031435 THOC5 232 105 2.2 NM 030576 LIMD2 1251 565 2.2 NM 015106 RAD54L2 316 143 2.2 NM 000487 ARSA 135 61 2.2 NM 001169109 SC02 270 122 2.2 NM 014205 ZNRF2 135 61 2.2 NM 001666 ARHGAP4 1033 467 2.2 NM 012345 NUFIP1 88 40 2.2 NM 001150 ANPEP 792 358 2.2 NM 004091 E2f2 261 118 2.2 NM 014698 TMEM64 130 59 2.2 NM 001145462 Yipf2 345 156 2.2 NM 001037161 ACOT1 214 97 2.2 NM 005137 DGCR2 471 213 2.2 NM 173832 Zfyve1 256 116 2.2 NM 001163323 CCDC120 42 19 2.2 NM 032622 Lnx1 42 19 2.2 NM 001001290 SLC35E3 84 38 2.2 NM 182633 ZNF749 42 19 2.2 NR 026717 5 STK38L 327 148 2.2 NM 001111039 ZNF192 435 197 2.2 NR 037874 ZMAT3 355 161 2.2 NM 079834 SCAMP4 196 89 2.2 NM 015285 Wdr90 117 53 2.2 NR 029417 SHMT2 1935 877 2.2 NM 024546 RNF219 417 189 2.2 NM 032854 COR06 75 34 2.2 NM 002224 ITPR3 37 17 2.2 NM 002736 PRKAR2B 75 34 2.2 NM 001142532 SH2D3C 37 17 2.2 NM 022553 1 Wasf3 262 119 2.2 NM 003902 FUBP1 408 185 2.2 NM 005190 CCNC 258 117 2.2 NM 015229 KIAA0664 699 317 2.2 NM 212533 ABCA2 145 66 2.2 NM 020937 Fanc 145 66 2.2 NM 001011 Rps7 3222 1462 2.2 NM 001146284 TCFL5 178 81 2.2 NM 007104 RPL10A 2448 1111 2.2 NM 001145839 MTFR1 141 64 2.2 NM 198486 RPL7L1 564 256 2.2 NM 005720 ARPC1B 1824 828 2.2 NM 152424 FAM123B 456 207 2.2 NM 006086 TU FM 1575 715 2.2 NM 001025604 ARRDC2 522 237 2.2 NM 004505 VAC 14 174 79 2.2 NM 014602 PIK3R4 409 186 2.2 NM 001142503 STAU1 508 231 2.2 NM 033071 SYNJ2BP 169 77 2.2 NM 080820 DTD1 400 182 2.2 NM 145004 ADAM32 33 fifteen 2.2

NM 138701 C7orf11 264 120 2.2 NM 001145268 FAM185A 33 fifteen 2.2 NM 001177702 IFT27 33 fifteen 2.2 NM 001134774 KLC2 66 thirty 2.2 NM 014067 MACROD1 66 thirty 2.2 NM 018049 PLEKHJ1 330 150 2.2 NM 175886 PRPS1L1 33 fifteen 2.2 NM 001039573 SEC14L1 264 120 2.2 NM 006949 STXBP4 33 fifteen 2.2 NM 001078651 TMEM143 33 fifteen 2.2 NM 001167734 Vnn1 33 fifteen 2.2 NM 003447 ZNF175 132 60 2.2 NM 145201 NAPRT1 259 118 2.2 NM 012455 Psd4 580 264 2.2 NM 001106 ACVR2B 321 146 2.2 NM 033418 METTL18 127 58 2.2 NR 033814 DLST 477 217 2.2 NM 052852 ZNF496 378 172 2.2 NM 152331 ACOT4 61 28 2.2 NM 003887 ASAP2 61 28 2.2 NM 001201335 C2orf3 123 56 2.2 NM 005211 CSF1R 61 28 2.2 NM 001001660 LYRM5 61 28 2.2 NM 005952 MT1X 61 28 2.2 NM 001130082 PLXNB1 61 28 2.2 NM 198155 C21orf33 838 382 2.2 NM 001172681 ZNF646 327 149 2.2 NM 001243130 RPL13 10660 4858 2.2 NM 006912 RIT1 147 67 2.2 NM 013373 Zfat 147 67 2.2 NM 015074 KIF1B 175 80 2.2 NM 032504 UNC93B1 640 292 2.2 NR 027856 CLK1 522 238 2.2 NM 001918 Dbt 318 145 2.2 NM 001130725 PSMB5 460 210 2.2 NM 001552 IGFBP4 1429 652 2.2 NM 017850 C1orf109 199 91 2.2 NM 024821 CCDC134 28 13 2.2 NM 007186 Cep250 741 338 2.2 NM 018217 EDEM2 399 182 2.2 NM 133509 RAD51B 28 13 2.2 NR 002309 RPS26P11 28 13 2.2 NM 001098509 SGSM2 199 91 2.2 NM 001195267 Ssh3 28 13 2.2 NM 001168215 TMEM99 57 26 2.2 NM 001242475 ZNF382 57 26 2.2 NM 198458 ZNF519 28 13 2.2 NM 006218 PIK3CA 195 89 2.2 NM 005934 MLLT1 1141 521 2.2 NM 002695 POLR2E 609 278 2.2 NM 021224 ZNF48 138 63 2.2 NM 024840 ZNF627 385 176 2.2 NM 138346 KIAA2013 766 350 2.2 NM 001190980 YIF1A 219 one hundred 2.2

NM 005586 MDFI 300 137 2.2 NM 002501 Nfix 705 322 2.2 NM 145265 CCDC127 162 74 2.2 NM 005113 GOLGA5 243 111 2.2 NM 001130970 NELF 162 74 2.2 NM 001077493 NFKB2 81 37 2.2 NM 006509 Relb 81 37 2.2 NM 001098 AC02 700 320 2.2 NM 001136158 OTUD5 376 172 2.2 NM 003846 Pex11b 214 98 2.2 NR 024022 SETMAR 133 61 2.2 NM 001040273 UBASH3B 1116 510 2.2 NM 003449 7 TRIM28 2671 1221 2.2 NM 152721 DOK6 52 24 2.2 NM 001040427 Hag h 52 24 2.2 NM 001198989 Nfs1 105 48 2.2 NM 001131026 Pex5 105 48 2.2 NR 037673 SERF2-C150RF63 210 96 2.2 NM 174914 UHRF1BP1L 105 48 2.2 NM 001202473 ZNF845 157 72 2.2 NM 001166237 GSDMD 706 323 2.2 NM 004995 MMP14 778 356 2.2 NM 183075 CYP2U1 205 94 2.2 NM 016025 METTL9 358 164 2.2 NM 001037171 ACOT9 76 35 2.2 NM 001193321 IKBKE 306 140 2.2 NM 000363 TNRC6B 483 221 2.2 NM 001134473 KIAA0182 660 302 2.2 NM 016360 TAF10 277 127 2.2 NM 001193307 SAMD9 378 173 2.2 NM 002883 RANGAP1 1057 484 2.2 NM 007323 Zhx1 201 92 2.2 NM 032184 SYNGR2 1728 791 2.2 NM 024555 Fbxl6 124 57 2.2 NM 153687 IKBIP 124 57 2.2 NM 001242827 Sirt5 124 57 2.2 NM 020197 SNAPC2 124 57 2.2 NM 138463 TLK1 517 237 2.2 NM 024596 Mcph1 220 101 2.2 NM 138804 C2orf65 264 121 2.2 NM 024793 CLUAP1 24 eleven 2.2 NM 019609 CPXM1 48 22 2.2 NM 173537 GTF2IRD2 48 22 2.2 NM 022070 HEATR6 360 165 2.2 NM 001134435 IQCG 24 eleven 2.2 NM 173546 KLHDC8B 72 33 2.2 NM 016027 LACTB2 216 99 2.2 NM 078628 Msl3 72 33 2.2 NM 002135 NR4A1 24 eleven 2.2 NM 013239 PPP2R3B 24 eleven 2.2 NM 022370 ROB03 72 33 2.2 NM 182756 SPDYE2 24 eleven 2.2 NM 001142634 SPDYE2L 24 eleven 2.2 NM 001242783 7 TRIM27 24 eleven 2.2

NM 017890 Vps52 24 eleven 2.2 NM 017984 ZDHHC11 24 eleven 2.2 NM 145238 ZSCAN5A 24 eleven 2.2 NM 001127183 CFLAR 379 174 2.2 NM 001077261 NCOR2 1134 520 2.2 NM 001002878 THOP1 519 238 2.2 NM 017858 Tkt 2287 1049 2.2 NM 001114618 MGAT1 1153 529 2.2 NM 199287 CCDC137 658 302 2.2 NM 138392 SHKBP1 327 150 2.2 NM 006184 NUCB1 630 289 2.2 NM 001083330 ZNF16 115 53 2.2 NM 022156 DUS1L 847 389 2.2 NM 145267 C6orf57 91 42 2.2 NM 001205029 MDM1 91 42 2.2 NM 001024678 LRRC24 159 73 2.2 NM 001100122 SBN02 318 146 2.2 NM 001164234 DDHD2 67 31 2.2 NR 036432 HERC2P3 67 31 2.2 NM 001243247 NPRL3 135 62 2.2 NM 144568 TMEM63A 135 62 2.2 NM 080732 EGLN2 535 246 2.2 NM 001005920 Jmjd8 357 164 2.2 NR 003662 RPSAP58 10575 4858 2.2 NM 001184970 Pacsin2 468 215 2.2 NM 004215 EBAG9 111 51 2.2 NM 024303 ZSWIM6 111 51 2.2 NM 001009939 Sept5 376 173 2.2 NR 038123 PSMA3 198 91 2.2 NM 014298 QPRT 198 91 2.2 NM 030912 TRMT112 570 262 2.2 NM 001105576 ANKRD58 43 twenty 2.2 NM 194441 BTN3A1 87 40 2.2 NM 001206879 CTDSP1 174 80 2.2 NM 019005 MIOS 304 140 2.2 NM 001174085 POLL 87 40 2.2 NM 004881 TPM2 43 twenty 2.2 NM 001199119 4 TRIM45 87 40 2.2 NM 006958 ZNF174 43 twenty 2.2 NM .002419 MAP3K11 628 289 2.2 NM 006114 TOMM5 387 178 2.2 NM 006901 MY09A 256 118 2.2 NM 016538 Sirt 7 276 127 2.2 NM 017457 CYTH2 315 145 2.2 NM 021976 3 Rxrb 63 29th 2.2 NM 001042462 TRDMT1 63 29th 2.2 NM 007113 TCP11L1 145 67 2.2 NM 015318 ARHGEF18 810 373 2.2 NM 020959 AN08 82 38 2.2 NM 005479 FRAT1 82 38 2.2 NM 000359 Thap6 82 38 2.2 NM 018210 CARKD 267 123 2.2 NM 004047 ATP6V0B 553 255 2.2 NM 001122956 Dbnl 922 425 2.2

NM 001103167 ZKSCAN1 1024 472 2.2 NM 024661 CCDC51 306 141 2.2 NM 138443 HAUS1 204 94 2.2 NM 001029989 KIAA0101 588 271 2.2 NR 026702 GLYCTK 160 74 2.2 NM 001165417 SLC25A13 199 92 2.2 NM 052853 ADCK2 238 110 2.2 NM 178557 NAT8L 516 238 2.2 NR 037653 ST3GAL2 258 119 2.2 NR 037791 RAB4B-EGLN2 555 256 2.2 NM 003743 NCOA1 316 146 2.2 NM 004958 MTOR 730 337 2.2 NM 001127397 ERLEC1 433 200 2.2 NM 001122772 AGAP2 570 263 2.2 NM 138795 ARL8A 175 81 2.2 NM 001183 ATP6AP1 858 396 2.2 NM 000397 CYBB 19 9 2.1 NM 201563 FCGR2C 19 9 2.1 NM 020400 LPAR5 19 9 2.1 NM 001159644 MCTP2 58 27 2.1 NM 001145785 MEF2B 19 9 2.1 NM 176796 P2RY6 39 eighteen 2.2 NR 029383 Pan3-as1 19 9 2.1 NM 001127692 PCCA 136 63 2.2 NM 002601 PDE6D 78 36 2.2 NM 014172 PHPT1 234 108 2.2 NM 002690 POLB 39 eighteen 2.2 NM 001199771 RDH5 19 9 2.1 NM 001037442 RUFY3 39 eighteen 2.2 NM 024583 SCRN3 175 81 2.2 NM 001145541 TEAD4 78 36 2.2 NM 025246 TMEM229B 19 9 2.1 NM 017955 CDCA4 385 178 2.2 NM 007311 TSPYL2 327 151 2.2 NM 153717 EVC 268 124 2.2 NM 002013 FKBP3 229 106 2.2 NM 021626 SCPEP1 381 176 2.2 NM 138639 BCL2L12 151 70 2.2 NM 014284 Ncdn 415 192 2.2 NM 022460 HS1BP3 132 61 2.2 NM 002473 MYH9 5848 2703 2.2 NM 001080432 Fto 337 156 2.2 NM 033416 IMP4 225 104 2.2 NM 003089 SNRPB 1345 622 2.2 NM 001032282 KLF10 205 95 2.2 NM 003025 SH3GL1 298 138 2.2 NM 018380 DDX28 259 120 2.2 NM 006059 Lamc3 426 197 2.2 NM 001012329 CTNNBIP1 166 77 2.2 NR 002182 NACAP1 333 154 2.2 NM 022648 TOM1 166 77 2.2 NM 006185 NUMA1 2640 1221 2.2 NM 001137601 ZBTB5 387 179 2.2 NM 006916 RPE 201 93 2.2

NM 003824 Fadd 382 177 2.2 NM 016458 1 FAM203A 127 59 2.2 NM 001136015 ANXA2 181 84 2.2 NM 015531 C2cd3 181 84 2.2 NM 001004720 NCK2 363 168 2.2 NM 022750 PARP12 235 109 2.2

Thus, an analysis was made of the suppression of the expression of oncogenes in transplanted hematopoietic cells transduced with a lentiviral vector that directs the synthesis of shRNA, which mediates the suppression of the activated oncogen AML1-ETO. It was shown that the transduction of Kasumi-1 cells using lentiviral vectors that direct the synthesis of specific shRNA, siRNA precursors complementary to the AML1-ETO oncogen, leads to a tenfold decrease in the expression level of this oncogen.

Using the approach using the deep sequencing method on the Illumina platform, about 2500 thousand genes were identified, the expression of which changes when the expression of the activated oncogen AML1-ETO is suppressed . From the presented list of genes, it seems possible to select genes that can be directly or indirectly involved in the processes associated with the development of acute myeloid leukemia. The study of the function of such genes using the proposed method provides an opportunity to examine the detected genes for their use as target genes for the diagnosis and treatment of AML.

Claims (2)

1. The nucleotide sequence of short hairpin RNA (shRNA) to suppress the expression of activated AML1-ETO oncogen in human leukemia cells, built into the lentiviral vector at the BamHI and EcoRI restriction sites encoded by the double-stranded DNA nucleotide sequence, which is:
shAE1SLP semantic
5′-r-gatccgCCTCGAAATCGTACTGAGActtcctgcaaTCTCAGTACGATTTCGAGGtttttg-3 ′
shAE1SLP-antisense
5′-p-aattcaaaaaCCTCGAAATCGTACTGAGAttgcaggaagTCTCAGTACGATTTCGAGGcg-3 ′. 2. A method for identifying target genes for the diagnosis and treatment of human leukemia, the expression of which is controlled by activated oncogenes, characterized in that in the culture of transplantable malignant cells of the Kasumi-1 line obtained from a patient with leukemia, the expression of activated AML1-ETO oncogen is suppressed by RNA- interference using the nucleotide sequence of short hairpin RNA according to paragraph 1, embedded in the lentiviral vector pLSLP-AML-ETO-shRNA, screen for gene expression profile in leukemia target cells, and genes whose expression profile is changing, are targets for the diagnosis and treatment of human leukemia.

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