RU2568908C1 - Method for producing therapeutic agent possessing antioxidant activity - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: herbal material is ground, extracted three times by alcohol water mixture; extracts are combined, concentrated under vacuum; the extract is cleaned by centrifugation, dried and ground in the certain environment. The total content of phenol compounds in the prepared agent in terms of the equivalent amount of rutin makes not less than 5%, whereas the resveratrol content makes not less than 0.005%.

EFFECT: high antioxidant activity of the therapeutic agent.

2 cl, 2 tbl

Description

The invention relates to pharmaceuticals and medicine, and relates to a method for producing funds from red leaves of cultivated grapes collected in the phase of the end of the vegetation of the plant and having antioxidant activity.

Cultivated grapes (Vitis vinifera L.) family Grapes (Vitaceae) - one of the most ancient cultivated plants of Eurasia, is a large vine, reaching 30-40 m in length. Cultivated grapes grow in temperate and subtropical regions. Currently, it is widely cultivated in the Caucasus, Central Asia, Moldova, Ukraine, and the southern regions of Russia.

Along with widespread use in the food industry, grapes are a promising source of biologically active substances.

A known method of obtaining a dry aqueous extract from the peel of dark grape varieties (Vitis vinifera L.), which is part of the drug "Vita-plant". The method consists in extraction with water by the method of percolation of crushed raw materials (peel of red grape varieties). The resulting liquid extract was dried by spray drying and then sieved. The dry extract is a dark red powder with a specific odor (patent RU 2274469, IPC A61K 36/78, 2004).

Also known is a method of obtaining a dry extract from the ridges of grapes with anti-alcohol action. The method consists in the following: grape ridges, previously dried at 40-50 ° C and crushed to particle sizes equal to 0.1-0.5 cm, extracted with a ratio of water and ethanol in the range 34-46: 6-14, temperature 45 -55 ° C, and the extract obtained is subjected to distillation at a temperature of 45-50 ° C and a residual pressure of 300-400 mm RT. Art. (patent SU 10772309).

The disadvantages of these methods is that water is used as an extractant. Since phenolic compounds of predominantly glycosidic nature go into aqueous extraction, in addition, the concentration of the aqueous extract takes a long time, during which the biologically active substances are exposed to heat. This circumstance can lead to a decrease in the pharmacological activity of the obtained extract.

Closest to the claimed invention for its intended purpose and in the stages of the technological process is a method for producing the main component of a composition suitable for spraying. This method (selected as a prototype) is that the leaves of red grapes are harvested, dried, crushed, cut into pieces and subjected to extraction for 6-10 hours with water or ethanol or a mixture thereof at elevated temperature and, if necessary, concentrated (RU 2482864 C2, IPC A61K 36/87, 2008).

In contrast to the prototype, the claimed invention uses red leaves of the cultivar (Vitis vinifera L.) of the Cabernet variety harvested in the phase of the end of the plant growing season as a raw material. During this period, instead of chlorophyll, which is necessary in the summer for photosynthesis, the plant begins to synthesize anthocyanins, which give the leaves a red color.

In addition, during this growing season, in order to adapt to cold weather, the plant produces increased amounts of resveratrol, which makes red leaves a more valuable source of biologically active substances.

The feasibility of collecting this plant material during the period of complete reddening of the leaves should be taken into account, since in the climatic conditions of our country most vineyards are located in the risky farming zone, and therefore, to improve wintering conditions before shelter, as a rule, red leaves are deliberately cut off, which is practiced in first of all in the Rostov region. At the same time, red leaves are secondary resources of viticulture and their collection does not harm the use of cultivated grapes as raw materials for the food industry and winemaking.

The purpose of the invention is to obtain funds from red leaves of cultivated grapes with antioxidant activity.

The goal is achieved in that the plant material (raw materials) is crushed to a particle size of 1-7 mm, preferably 3-5 mm; extracted three times with 10-70% aqueous-alcoholic mixture, preferably 20-50%; the resulting extract was combined and concentrated in vacuo. The concentrated extract is then purified by centrifugation at 3000-7000 rpm, preferably 5000-7000 rpm. The purified extract is dried and ground.

Get the finished product - dry extract of red leaves of cultivated grapes with the content of the amount of phenolic compounds in terms of rutin of at least 5%, resveratrol of at least 0.005%.

Cultivated grapes are the most common type, widely cultivated in the Russian Federation and successfully used for selection. Currently, about 4 thousand grape varieties are known. In this regard, to substantiate the choice of medicinal raw materials, we conducted a study of red leaves of widely cultivated grape varieties, such as Saperavi, Golubok, Cabernet, Krasnostop Zolotovsky. In the indicated varieties, the content of extractives and the sum of phenolic compounds in terms of rutin and resveratrol were determined (Table 1).

According to the data in Table 1, the largest amount of extractives, phenolic compounds, and resveratrol is found in the red leaves of the Cabernet cultivar.

Along with this, in vitro biotesting was carried out using glutathione reductase and pyruvate kinase reactions of dry extracts obtained from red leaves of cultivated grapes of 4 varieties (L.A. Oganesyan et al. // Drinks. Technologies and Innovations. - 2012. - No. 10 . - S. 63-65).

Based on biotesting, it was found that Cabernet varieties have a higher experimental reaction rate and thus have a more pronounced antioxidant effect (Table 2).

Thus, as a raw material for obtaining funds with antioxidant activity, it is preferable to use red leaves of the Cabernet cultivar.

Claims (2)

1. The method of obtaining funds with antioxidant activity from grape leaves, including grinding plant materials, extraction with a water-alcohol mixture, concentration, characterized in that as the plant materials used are red grape leaves of the Cabernet cultivar, collected at the end of the plant growing season, which are crushed to a particle size of 3-5 mm, extracted three times with a 20-50% aqueous-alcoholic mixture, the extracts are combined and concentrated in vacuo, the concentrated centri by fugging at 5000-7000 rpm, dried and crushed. 2. The method according to p. 1, where the total content in the resulting product of the sum of phenolic compounds in terms of rutin is at least 5% and the content of resveratrol is at least 0.005%.