RU2483310C1 - Method for prediction of high risk of forming chronic otorhinolaryngologic pathology in children of 3-7 years old - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: child's mixed saliva collected in the morning on an empty stomach for 10 minutes after mouthwash with boiled water at room temperature is examined for calcium (Arsenazo III) and magnesium (xylidene blue) by the colorimetric method. Then, the concentrations of calcium (Ca), mmol/l is related to the concentration of magnesium (Mg), mmol/l. If the Ca/Mg ratio is 2.5 and more, the child is referred to a high-risk group of forming a chronic otorhinolaryngological pathology.

EFFECT: prediction of a high risk of forming the chronic otorhinolaryngological pathology in the child of 3-7 years old.

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Description

The invention relates to the field of medicine, namely pediatrics, and can be used to predict an increased risk of chronic otorhinolaryngological pathology in children 3-7 years old.

Recently, there has been a tendency to the early formation of chronic pathology in children. In the period of 3-7 years in children, the formation of adaptive regulatory processes and the specialization of immunity. The relevance of the development of the method is determined by the high frequency of acute respiratory infections in children of this age group and a significant increase in the formation of chronic otorhinolaryngological pathology in them. Often ill children of preschool age account for 40 to 70%. Every third child who is often ill has a chronic otorhinolaryngological pathology. Often children with acute respiratory infections due to their significant prevalence have become not only a medical, but also a social problem (1, 2, 3).

Closest to the technical nature of the claimed method is a "Method for predicting acute morbidity of the nasopharyngeal tract in children" / Zhdanova LA, Poberskaya B.A. // BI N18. -1995. S.209, patent N2038601.

By determining lysozyme in mixed saliva, the incidence of infectious diseases is predicted. Saliva is collected in the morning on an empty stomach after a preliminary rinse of the oral cavity with boiled water. In the collected saliva, lysozyme activity is determined. The determination of lysozyme in mixed saliva is carried out by the nephelometric method with a daily culture of Micrococcus lysodeicticus according to V.G. Dorofeychik (1984).

Differences in the content of the quantitative level of lysozyme in sick and healthy children are significant and make up 34.2 3.7 standard units in the group of sick children, and 11.4 1.43 standard units in sick children. (p <0.001), which allows using this criterion to predict the development of exacerbation of ENT pathology. The child determines the level of lysozyme in arbitrary units and with an increase of more than 20 arbitrary units, an exacerbation of ENT pathology is predicted.

The disadvantages of this method are

1) the method for determining lysozyme requires a bacteriological laboratory, since it is assumed to use a culture of microorganisms;

2) lysozyme indices have a significant range of values, and when screening their quantitative indices are more important when comparing the incidence of children's groups, therefore, for an individual prognosis, it is more important to search for integral indices reflecting the functional state of local immunity in a particular child.

The aim of the present invention is to develop a method for predicting an increased risk of chronic otolaryngological pathology in children 3-7 years old.

The technical result, the achievement of which the creation of this invention is directed, is the determination of calcium (Arsenazo III method) and magnesium (xylidyl blue method) in mixed saliva of a child (collected in the morning on an empty stomach, for 10 minutes after rinsing the mouth with boiled water at room temperature) using the colorimetric method with using diagnostic test systems for biochemical research "BioChemMac" on a biochemical analyzer ABX MICROS 60 (France, 2001), after which the coefficient of the ratio of the concentration of calcium (Ca) in mmol / L to the concentration of magnesium (Mg) in mmol / L.

With a value of the ratio of concentration ratios Ca / Mg = 2.5 and higher, the child is considered to be at increased risk of developing chronic otorhinolaryngological pathology and is referred for consultation with an otorhinolaryngologist.

New previously unknown signs of the claimed method for predicting an increased risk of chronic otolaryngological pathology in children 3-7 years old are

1) predicting an increased risk of the formation of chronic otorhinolaryngological pathology in children 3-7 years old by the coefficient of the ratio of calcium and magnesium concentrations in the mixed saliva of the child,

2) prognostic parameters of the coefficient are established, with a value of = 2.5 and higher, an increased risk of the formation of a chronic otorhinolaryngological pathology in a particular child is predicted.

The essence of the proposed method is illustrated by the following examples.

Example 1. The child is 4 years old, formalized card No. 84.

A child from 1B, 1P, proceeding physiologically. Breastfeeding up to 6 months. I went to DDU from 3.5 years old, until DDU I had acute respiratory infections 2-3 times a year, without complications. 2 weeks after the start of the visit, the child had acute respiratory infections complicated by acute otitis media, and a relapse of acute respiratory infections a month later. The child was referred to an immunologist. The analysis of saliva.

The concentration of calcium in the mixed saliva of the child is 0.34 mmol / l.

The concentration of magnesium in the mixed saliva of the child is 0.11 mmol / l.

The ratio of calcium to magnesium concentration in mixed saliva is 0.34 / 0.11 = 3.1.

A high risk of the formation of chronic otorhinolaryngological pathology was predicted. The child is referred for consultation with an otorhinolaryngologist. The doctor diagnosed with chronic adenoiditis. The forecast came true.

Example 2. A child of 5 years, a formalized card No. 47.

B2, P1. Cesarean section. Breastfeeding up to 2 years. In DDU from 3 years. Until 3 years he was ill 3-4 times a year. From 3 to 6 years he was sick 7 times a year. Directed to the immunologist after the next ARI. The analysis of saliva.

The concentration of calcium in the mixed saliva of the child is 0.61 mmol / L.

The concentration of magnesium in the mixed saliva of the child is 0.4 mmol / l.

The ratio of the concentration of calcium to the concentration of magnesium in mixed saliva = 1.53.

A favorable forecast was predicted. The child was examined by an otorhinolaryngologist. Chronic otorhinolaryngological pathology was not detected. The forecast was confirmed.

Bibliography

1. Kuchma V.R. School Hygiene and Health Guide. M .: Russian Association of Public Health, 2000. - P.18.

2. Albitsky V.Yu., Baranov A.A. Often sick children, clinical and social aspects. Ways of healing. - Saratov, 1986. - 183 p.

3. Samygina G.A. Often sick children: problems of pathogenesis, diagnosis and therapy // Consilium medicum. - Application. "Pediatrics". - 2004. - N2. - C.3.

Claims (1)

A method for predicting an increased risk of formation of chronic otolaryngological pathology in children 3-7 years old, including carrying out a biochemical study of mixed saliva of a child, characterized in that calcium and magnesium are determined in a mixed saliva of a child by the colorimetric method and the ratio of the concentration of calcium (Ca) in mmol / l is calculated to the concentration of magnesium (Mg) in mmol / l; with a value of the ratio of concentration ratios Ca / Mg = 2.5 and higher, the child is considered to be at increased risk for the formation of a chronic otorhinolaryngological pathology in him.