RU2448738C2 - Method of obtaining disposable atraumatic application - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to medicine. Described is method of obtaining disposable atraumatic application, which includes oxidation of medical gauze with obtaining dialdehidecellulose, further immobilisation of medications on it, oxidation being carried out to 6.0+0.33% degree, after that, obtained mixture is grinded until microfibrous form, modified by medicinal components, is obtained, mixed with powder of gel-forming reagent, mixture is intensively mixed with water, then, solution is poured into forms, dried and obtained gel plates are connected with paper layers, made from wood cellulose, by sewing method.

EFFECT: application ensures accelerated purification of wounds from purulent-necrotic masses and reduction of wound-healing terms.

2 cl, 5 ex

Description

The invention relates to medicine, specifically to surgery, and emergency care.

The level of technology.

A known method of obtaining dressings "Napkins Filatov-Ryltsev." Napkins consist of a modified medical gauze with one or two drugs chemically immobilized on it (trypsin, trypsin and lysozyme, trypsin and insulin). The speed of cleansing of purulent-necrotic wounds of various origin for wipes containing two drugs is on average 2.4 days (see, for example, patent RU No. 2142818).

Known material with biological activity, a method for its preparation and a therapeutic agent based on it, which is a modified medical gauze with three drugs co-immobilized on it (trypsin, insulin, lysozyme). The speed of cleansing of purulent-necrotic wounds of various origin with this material is 1.8 days (see, for example, patent RU No. 2357753, analogue).

Known textile material for stopping bleeding and a method for producing it, which is a modified medical gauze with coagulation factors immobilized on it: έ-aminocaproic acid, fibrinogen, thrombin, gelatin and the bacteriolytic enzyme lysozyme. The rate of stopping bleeding with this material is 1 min. In the prototype - 2 minutes (see, for example, patent RU No. 2235539).

Known from the prior art materials are not without drawbacks. Materials containing trypsin and insulin immobilized on modified medical gauze, trypsin and lysozyme, lysozyme, insulin, trypsin, have a fairly high adhesion to the wound surface. The force of separation of such material from the wound surface is 99 g. In some cases, this leads to injury to the wound surface, repeated bleeding and pain. For this reason, some patients, especially children, refuse treatment with such napkins.

The aim of the present invention is to remedy these disadvantages.

SUMMARY OF THE INVENTION

The present invention provides a new material with wound healing and hemostatic properties at the same time.

The first aspect of the present invention is a therapeutic material based on DAN, with drugs immobilized on it, providing accelerated cleansing of wounds from purulent-necrotic masses and reducing the healing time of wounds.

Each drug contributes to speeding up the process of cleansing and healing wounds. Lysozyme is a bacteriolytic enzyme with antimicrobial activity, lysing certain structures of the cell wall, mainly gram-positive microorganisms, which leads to their death. Mexidol is an antioxidant that inhibits free radical reactions, inhibits the processes of peroxidation and thus contributes to the acceleration of wound healing.

The second aspect of the present invention is a material that provides a quick stop bleeding. Gelatinol and έ-aminocaproic acid provide a hemostatic effect due to the formation of a blood clot, activation of blood coagulation and a decrease in the antiplatelet ability of the vascular wall.

A third aspect of the present invention is that these medicinal substances chemically bound to the carrier, dialdehyde cellulose, are in the form of microfibers.

A fourth aspect of the present invention is a dry gel plate based on acrylic compounds, in the structure of which are microfibers of DAC with immobilized medicinal substances. The presence of the gel component not only ensures the presence of drugs in the “depot” material, but also carries a sorption load, having a high absorbency.

A fifth aspect of the present invention is the presence of an absorbent layer in the form of filter paper based on wood pulp. This layer has a dual function - it absorbs the wound and is simultaneously a protective layer.

Thus, the present invention provides a new therapeutic material for healing wounds of various etiologies and stop bleeding, with various types of biological activity. The material is able to shorten the healing time of wounds. Thanks to the gel, the material is easily removed from the damaged surface without injuring it and without causing pain to the patient. The treatment material, which is a gel plate with microparticles of dialdehyde cellulose with sizes from 1 to 25 microns with lysozyme, mexidol, gelatinol, and aminocaproic acid immobilized on them, impregnated with wound, is easily removed from the surface of the cleaned wound. The protective layer made of wood pulp is connected to the treatment layer by sewing. No adhesion. This explains the mechanism of high non-invasiveness of the proposed material.

The presence in the gel plate of microparticles with gelatinol and aminocaproic acid immobilized on it contributes to a quick and effective stop of bleeding.

DETAILED DESCRIPTION OF THE INVENTION

The invention is further described by examples of its implementation.

Example 1

12 l of distilled water are poured into the reactor, 178.0 g of iodic acid are added and stirred until complete dissolution of iodic acid. 3 l of distilled water are poured into the neighboring reactor, 29.4 g of sodium hydroxide are added and stirred until the sodium hydroxide is completely dissolved. The solutions are poured together into the first reactor, gradually pouring the alkali solution to the acid solution, stirred and the pH of the solution is determined on a pH meter. The pH of the prepared solution is adjusted to a value of 5.5 with a solution of iodic acid or sodium hydroxide.

Example 2

3.0 kg of medical gauze was placed in reactor 1 with sodium periodate, closed with a lid and left for 1 h at room temperature in the dark with periodic stirring. 1 h after soaking, the material is passed through squeezing rolls with a pre-installed squeezing module 1.0-1.5, while the squeezing solution is returned to the reactor 1. The squeezed material is again immersed in the solution in the reactor 1, closed with a lid and left at room temperature in darkness for 18 hours

After the oxidation is completed, dialdehyde cellulose is squeezed out, passing through squeezing rolls, washed in a bath "B" at least 8 times with 30 l of distilled water per day. The sodium iodate solution is collected and sent once a quarter for evaporation.

The last portion of the wash water is checked for the presence of iodic acid on a spectrophotometer using distilled water (wavelength - 222 nm) as a control. Dialdehyde cellulose is dried in a ventilated, clean room in air at room temperature.

Example 3

17.1 g of distilled water are poured into the reactor and 171 g of gelatinol (2.14 l of 8% gelatinol solution in isotonic sodium chloride solution), 142.5 g of амин-aminocaproic acid, 17.1 g of lysozyme and 17.1 g of mexidol are dissolved (342 ml of a solution with a concentration of Mexidol 50.0 mg / ml) with periodic stirring.

After 2 hours, the web is squeezed on rolls (spin module 1.5 - 2.0) and dried in air at room temperature. The dried web arrives at section 1 — the manufacture of a microfiber form of a material with medicinal substances that has hemostatic, antioxidant, and bactericidal activity.

Example 4

The dried cloth on a cutting table is cut into small pieces and subjected to grinding in a high-speed rotary mill ZM 200 to a microfiber shape, to particles from 40 to 80 microns in size (75% yield). Get 2.4 kg of microfibre, with medicinal substances, with hemostatic, antioxidant and bactericidal activity.

Example 5

2.0 l of distilled water heated to a temperature of 90 ± 5 ° C is poured into reactor 4, 120.0 g of Temposcrin dry powder are slowly added with continuous stirring. The reaction mass is stirred until the powder is completely dissolved, left at room temperature for 2 hours to stabilize the mass to a homogeneous state and cool to room temperature.

1.0 l of distilled water at room temperature 18-20 ° C is poured into the reactor 5 and 20.0 g of microfibre containing medicinal substances are added with continuous stirring. The reaction mass is stirred for 10-15 minutes until a homogeneous suspension is obtained (without lumps) and added to the reactor 4 with stirring. The resulting mass is continuously stirred for another 20-30 minutes and sent to the formation of gel plates.

The resulting mass of a hydrogel solution containing microfibers with medicinal substances is poured into the form of the required size (I, II or III), kept at room temperature for 30 minutes, dried in a heating cabinet at a temperature of 35-37 ° C and left to stand in air until completely dried (about 24 hours). For example, at a given load, 21 ml of gel mass is poured into 10 × 10 cm molds, and 140 gel plates of ~ 1.0 g weight, 10 × 10 cm in size are obtained.

Filter laboratory paper of the brand "F" is cut into blanks-plates of the appropriate size. The gel plate is connected to several layers (from 1 to 3) of filter paper of the appropriate size by sewing.

The advantage of the invention.

The tool can be used to treat purulent-necrotic wounds of any origin, including long-term non-healing wounds, such as wounds and ulcers against trophic disorders.

The tool can be used to stop both capillary and parenchymal bleeding.

The product does not need additional moisturizing while on the wound due to the presence of a gel layer that swells in the wound and maintains a moist environment.

The terms of wound healing coincide with the analogue. The timing of stopping bleeding coincides with the prototype. This means that both the therapeutic and hemostatic components are not antagonists when used together, therefore, the proposed material has a multifunctional effect, which allows us to recommend it for the treatment of wounds and stop bleeding in emergencies - natural and man-made disasters, accompanied by massive damage to people and in the field.

Universal properties make it possible to use them also in specialized medical institutions - purulent and traumatological departments of city and regional hospitals, at ambulance stations, clinics and at home.

Claims (2)

1. A method of obtaining an application of atraumatic disposable, including the oxidation of medical gauze to obtain dialdehyde cellulose, the subsequent immobilization of drugs on it, characterized in that the oxidation of gauze is carried out to a degree of 6.0 ± 0.33%, the resulting mixture is ground to obtain a microfiber modified form medicinal components, mixed with an aqueous solution of a reagent forming a gel, the mixture is intensively mixed until a homogeneous suspension is obtained, then the solution is poured into molds, dried and obtained nye gel plate sewing method is combined with layers of paper made from wood pulp. 2. The method according to claim 1, characterized in that the modified microfiber cellulose contains the following components, wt.%:
dialdehyde cellulose 88.9 ± 0.3 gelatinol 5.4 ± 0.3 ε-aminocaproic acid 4,5 ± 0,3 lysozyme 0.4 mexidol 0.8