RU2440146C2 - Disinfectant - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to veterinary, in particular to preparations for disinfection of objects of veterinary supervision against disease-causing microbes. Disinfectant includes glutaraldehide, glyoxal, alkylmethylbenzylammonium chloride and water, alkyldimethylethyl-benzylammonium chloride and anionic surface active substance, with the following component ratio, (wt %): glutaraldehide 3.0-4.0; glyoxal 2.5-2.8; alkylmethylbenzylammonium chloride 3.0-4.0; alkyldimethylethylbenzylammonium chloride 4.0-5.0; anionic surface active substance 8.0-9.0; water to 1 l. With application of 2% concentration disinfectant, consumption 400-500 ml/m² and 60-120 minute exposition complete disinfection of objects against pathogenic organisms is achieved, the same effect being obtained with 3-% concentration of preparation and impact during 30-60 minutes.

EFFECT: invention ensures increase of disinfecting effect.

2 tbl, 4 ex

Description

The invention relates to veterinary medicine, in particular to means for disinfecting objects of veterinary surveillance from pathogenic microbes.

A disinfectant with a washing effect is known, including as active ingredients: alkyldimethylbenzylammonium chloride (HOUR) and glutaraldehyde, as well as a nonionic surfactant (see reference: Disinfectants under the brand name "Lumax and Teko", M., 2008 , p.26).

However, the known agent does not have a high disinfectant activity.

The closest analogue (see reference book: Disinfectants under the brand name “Lumax and Teko”, M., 2008, p. 61) is a disinfectant with a detergent effect, it contains alkyl dimethylbenzylammonium chloride, the aldehyde complex: glutaraldehyde, glyoxal, aldehyde, nonionic surfactant, in the following ratio of components in 1 l of water (wt.%):

- glutaraldehyde 4.0 - glyoxal 2,8 - Alkyldimethylbenzylammonium chloride 4.0 nonionic surfactant is present - water rest

The drug is used at 2% and 3% concentration with an exposure of 120 minutes, the flow rate of the working solution is 500 ml / m ² .

However, the known disinfectant has a low disinfecting effect against spore forms.

The technical result is to increase the disinfecting effect, which is achieved by the fact that the disinfectant includes glutaraldehyde, glyoxal, alkyl dimethylbenzylammonium chloride and water, additionally contains alkyl dimethylethylbenzylammonium chloride and anionic surfactant, in the following ratio of components in 1 liter of water (wt.%):

- glutaraldehyde 3.0-4.0 - glyoxal 2.5-2.8 - Alkyldimethylbenzylammonium chloride 3.0-4.0 - Alkyldimethylethylbenzylammonium chloride 4.0-5.0 - anionic surfactant 8.0-9.0 - water up to 1 l

Studies have shown that when a quaternary ammonium compound (QAC) is added to a known disinfectant, an alkyldimethylethylbenzylammonium chloride, a synergy of substances is observed, namely, potentiation, this combination of active substances is more pronounced than the sum of the effects of each component. The addition of an anionic surfactant reduces the surface tension of the solution, which contributes to a better interaction of the solution with the object (wetting and sticking), facilitating the diffusion of active substances into the microbial cell, thus achieving a higher bactericidal effect of the proposed disinfectant, which has activity against gram-positive and gram-negative bacteria, including mycobacterium tuberculosis and spore.

Using the drug in the form of a working solution of 2% concentration, at a flow rate of 400-500 ml / m ² with an exposure of 60-120 minutes, complete disinfection of objects from pathogenic microorganisms is achieved, the same effect is obtained when using the drug in the form of a working solution of 3% concentration with an exposure of 30-60 minutes.

Alkyldimethylethylbenzylammonium chloride belongs to the group of quaternary ammonium compounds possessing a membrane-attacking mechanism of suppression of microorganisms. These drugs destroy the biopolymers that make up the cell membrane. As a result, lysis (destruction) of the microbial cell occurs. Quaternary ammonium compounds - cationic surfactants, antistatic agents with antifungal and antibacterial effects, conditioners. They have good emulsifying and wetting properties, moderate foaming and washing ability.

Surfactants (surfactants) are polar organic compounds, the polarity of surfactants is due to the structure of molecules consisting of two parts with different properties. One part of the molecule is hydrophobic (water-repellent), which promotes the dissolution of surfactants in oil, and the other is hydrophilic, promotes the dissolution of surfactants in water. The various molecular and surfactant structures give washing solutions a complex of properties that determine their washing effect: wetting, emulsifying, dispersing, solubilizing and stabilizing abilities.

The proposed surfactant belongs to the group of anionic, the use of which causes a decrease in the surface tension of the solution, as a result of which the contact area of the active substances with the microbial cell increases.

Example 1. The selection of an effective concentration of alkyldimethylethylbenzylammonium chloride in 1 liter of water 1.0; 3.0; 4.0 and 5.0 wt.% By adding to a disinfectant (composition 1). Then prepared working solutions of 1%, 2% and 3% concentration, to determine more effective.

A working solution of 1% concentration contains in 1 liter of water, wt.%:

glyoxal - 0.028;

glutaraldehyde - 0.04;

alkyldimethylbenzylammonium chloride - 0.04;

alkyldimethylethylbenzylammonium chloride - 0.01; 0.03; 0.04; 0.05.

Working solution of 2% concentration in 1 liter of water, wt.%:

glyoxal - 0.05;

glutaraldehyde - 0.08;

alkyldimethylbenzylammonium chloride - 0.08;

alkyl dimethylethylbenzylammonium chloride - 0.02; 0.06; 0.08; 0.1.

Working solution of 3% concentration in 1 liter of water, wt.%:

glyoxal - 0.084;

glutaraldehyde - 0.12;

alkyldimethylbenzylammonium chloride - 0.12;

alkyl dimethylethylbenzylammonium chloride - 0.03; 0.09; 0.12; 0.15.

The test of the disinfectant was carried out by irrigation with working solutions of various concentrations of wooden and cement test objects contaminated with suspensions of test cultures: St. aureus, B. cereus, B. subtilis, M. bovis, M. avium, M. scrofulaceum, in the amount of 1 ml of the 2-billionth suspension of cells (1 ml per 1 test object), prepared according to the industry standard turbidity (10 units ) After an hour and a half drying of the suspension of test cultures, test solutions of the disinfectant (1 composition) were applied to the test objects at a flow rate of 500 ml / m ² with an exposure of 30, 60, 120 minutes. At the same time, they put control over the viability of test cultures. Then, sterile distilled water was used to prepare swabs from the surface of test objects, the centrifugate was sown on standard media for culturing bacteria, depending on the test cultures. Crops were incubated in an incubator at 37 ° C. Initial counting was carried out after 24 hours, final after 7, 30, 60 days for test cultures of mycobacteria. At the same time, the viability of the test cultures was monitored.

The test results of composition 1 are presented in table No. 1

Table number 1 Comparative characteristics of the bactericidal activity of the proposed disinfectant and prototype Drug concentration Concentra-
nation
ADMEBA Exposure
min Consumption rate, ml / m ² Test microorganisms St. aureus B. cereus B. subtilis M. bovis M. avium M. scrofula-
ceum one 2 3 four 5 6 7 8 9 10 thirty - + + + + + 0.01 60 - + + + + + 120 - + + + + + thirty - + + + + + 0,03 60 - + + + + + 1,0 120 500 - + + + + + thirty - + + + + + 0.04 60 - + + + + + 120 - + + + + + thirty - + + + + + 0.05 60 - + + + + + 120 - + + + + + thirty - + + + + + 0.02 60 - + + + + + 2.0 120 500 - + + + + + thirty + + + + + 0.06 60 - + + + + + one 2 3 four 5 6 7 8 9 10 120 - + + + + + thirty - + + + + + 0.08 60 - + + + + + 120 - - - - - - thirty - + + + + + 0.1 60 - + + + + + 120 - - - - - - thirty - + + + + + 0,030 60 - + + + + + 120 - + + + + + thirty - + + + + + 0,090 60 - + + + + + 120 500 - - - - - - 3.0 thirty - + + + + + 0.12 60 - + + + + + 120 - - - - - - thirty - + + + + + 0.15 60 - - - - - - 120 - - - - - - thirty - + + + + + Prototype 60 500 - + + + + + 120 - + + + + + Water control + + + + + + Note: "+" - the presence of crop growth; “-” - lack of crop growth.

Bacteriological control and experiments were carried out in accordance with the Methodological instructions: “On the procedure for testing new disinfectants for veterinary practice”, approved by the GUV Gosagroprom of the USSR of January 7, 1987.

From table 1 it is seen that the drug in 1% and 2% concentration when the content of alkyldimethylethylbenzylammonium chloride is 0.01-0.08 wt.%, With exposures of 30, 60, 120 minutes does not have a bactericidal effect against mycobacteria and spores, working a solution of 2% concentration with a content of alkyldimethylethylbenzylammonium chloride of 0.1 wt.%, with an exposure of 120 minutes, exhibits bactericidal actions against tuberculosis and spore mycobacteria. The drug in 3% concentration containing 0.15 wt.% Alkyldimethylethylbenzylammonium chloride completely disinfects test objects from mycobacteria and spore microorganisms within 60 minutes.

Experimentally proved the effective content of alkyl dimethylethylbenzylammonium chloride 4-5 wt.% In 1 liter of water.

Example 2. Conducted the selection of the effective concentration of anionic surfactant in 1 liter of water 1.0; 5.0; 7.0; 8.0 and 9.0 wt.%. This substance was added to the proposed tool (composition 2). Next, working solutions of 2% and 3% concentration were prepared.

A working solution of 2% concentration contains in 1 liter of water, wt.%:

glyoxal - 0.05;

glutaraldehyde - 0.08;

alkyldimethylbenzylammonium chloride - 0.08;

alkyldimethylethylbenzylammonium chloride - 0.1;

anionic surfactant - 0.02; 0.1; 0.14; 0.16; 0.18.

A working solution of 3% concentration contains in 1 liter of water, wt.%:

glyoxal - 0.084;

glutaraldehyde - 0.12;

alkyldimethylbenzylammonium chloride - 0.12;

alkyldimethylethylbenzylammonium chloride - 0.15;

anionic surfactant - 0,030; 0.15; 0.21; 0.24; 0.27.

Tests are carried out for the same crops and test objects (wood, cement) as in example 1, in accordance with the above methodology. The test results are presented in table No. 2

Table number 2 Comparative characteristics of the bactericidal activity of the proposed disinfectant and prototype The concentration of the working solution,% Concentration of anionic surfactants Exposure, min Consumption rate, ml / m ² Test microorganisms St. aureus B. cereus B. subtilis M. bovis M. avium M. scrofula-ceum thirty - + + + + + 0.02 60 - + + + + + 120 - - - - - - thirty - + + + + + 0.1 60 - + + + + + 120 - - - - - - thirty - + + + + + 2.0 0.14 60 500 - + + + + + 120 - - - - - - thirty + + + + + 0.16 60 - - - - - 120 - - - - - thirty - + + + + + 0.18 60 - - - - - - 120 - - - - - - thirty - + + + + + 0,030 60 - - - - - - 120 - - - - - - thirty - + + + + + 0.15 60 - - - - - - 120 - - - - - - thirty + + + + + 3.0 0.21 60 500 - - - - - - 120 - - - - - thirty - - - - - - 0.24 60 - - - - - - 120 - - - - - - thirty - - - - - - 0.27 60 - - - - - - 120 - - - - - - thirty - + + + + + Prototype 60 500 - + + + + + 120 - + + + + + Water control + + + + + + Note: "+" - the presence of crop growth; “-” - lack of crop growth.

From table No. 2 it is seen that a 2% concentration of the drug containing anionic surfactant in 0.18 wt.%, Has bactericidal activity against mycobacteria and spore within 60-120 minutes, the drug in 3% concentration with an active substance content of 0.21-0.24 wt.% completely disinfects test objects from pathogenic microorganisms within 30-60 minutes.

Experimentally proved the effective content of surfactant in 1 liter of water 8-9 wt.%. Studies of the bactericidal activity of known and proposed disinfectants found that the new tool is 2-2.5 times more effective than the known.

Example 3. Toxic effect.

The toxicological characteristics were studied on white mice weighing 18-20 g, guinea pigs weighing 250 g and rabbits - 2 kg.

The studies were conducted in accordance with the guidelines "On the study of the toxicity of disinfectants intended for use in veterinary medicine" (1985).

It was found that for white rats LD ₅₀ = 1200 mg / kg; LD ₁₀₀ = 1600 mg / kg, for white mice LD ₅₀ = 1150 mg / kg; LD ₁₀₀ = 1550 mg / kg.

Local irritant effect on the skin.

The purpose of this study was to establish the ability of substances to penetrate intact skin and cause a toxic effect.

The experiments were carried out on guinea pigs and rabbits. In each group, 6 clinically healthy animals were taken, having previously cut their hair.

The solution of the composite mixture was applied in the form of an application once and ten times. The area of the skin area (total) for applying the drug in rabbits was 70 cm ² , in guinea pigs 20 cm ² . The dose of the drug was determined at the rate of 1 ml (20 mg) per cm ² .

The skin reaction was taken into account after 1 and 16 hours, and then daily for 14 days was compared with a symmetrical skin area of the same animal.

After a single application, a mild (pink tone) erythema of the skin was noted, which disappeared after 4-5 hours.

Tenfold application was accompanied by moderate erythema, which disappeared on the 6-8th day.

The thickness of the skin fold increased in guinea pigs by 0.3 mm, in rabbits by 0.74 mm (skin edema was estimated at 1 point).

When conducting a skin test by applying the test solution once and ten times, there was no concern, loss of appetite, or other deviations from the norm and death of the animals.

Local irritating effect of the drug on the mucous membrane of the eyes.

The studied drug was applied using a pipette into the conjunctival sac (rabbits), once in two drops. The reaction of the mucous membrane of the eye was taken into account immediately after exposure to the drug, then after 1 hour, then daily.

In rabbits, after application of the concentrate and 5% concentration of the drug, edema of the eyelids, excretion with moistening of the eyelids and hair adjacent to the eyelids were noted. No clouding of the cornea. These phenomena were observed within 3-4 days from the time of application of the drug. There were no other changes in rabbits during the observation period (10 days), 3-4% concentrations of the drug did not have visible changes in the mucous membranes of the eye.

According to the classification of the degree of hazard of chemicals (GOST 12.1.007-76), a washing universal concentrate with a disinfecting effect can be attributed to the third hazard class (moderately toxic compound).

Example 4. Production testing of preventive disinfection of premises was carried out in the conditions of subsidiary farms of military units during technological breaks in the period from 2006 to 2007. During this period, 300 thousand m ^{2 of} land were treated.

Wet disinfection of objects of veterinary and sanitary supervision was carried out using the filling station ARS-14K.

Microbial contamination of air before disinfection was 5–9 times higher than the permissible norms established for livestock buildings.

Flushing results confirmed the presence of a diverse microflora represented by bacteria of the genera Staphylococcus (30.66%), Escherichia (27.55%), Bacillus (24.76%), Streptococcus (3.12%), Proteus (2.2%), Pseudomonas (1.8%), Klebsiella (1.25%), Morganella, Salmonella, Yersinia (1.43%) and Penicillium, Thamnidium, Cladosporum (7.21%).

The proposed disinfection modes included processing rooms (surfaces) with working solutions of the drug at 2% and 3% concentration, using special equipment of the filling station (ARS-14K), once, at a flow rate of 500 ml / m ² , with an exposure of 1.5-2 hours, both with preliminary wet mechanical cleaning with tap water, and without it.

Working solutions were prepared in tap water or artesian water with a temperature of 18-20 ° C, thoroughly mixed and poured into an ARS-14K container immediately before disinfection.

Assessment of the quality of the disinfection of the premises was carried out according to the methodology described in the "Rules for the disinfection and disinfection of objects of state veterinary supervision" dated 15.07.02.

Multiple microbiological quality control of the proposed disinfection regimes, carried out on the basis of the bacteriological laboratory of military unit 34148 (SEO military unit 57343) and the laboratory of veterinary and sanitary measures and nutrient media of VNIIBTZH RAAS, indicates that the disinfection at the objects of veterinary and sanitary surveillance was carried out satisfactorily.

The disinfection criterion was considered the absence of growth of sanitary-indicative microflora (irrigation of surfaces with working solutions of the test drug) in the presence of growth of the latter from washouts from control (water irrigation) surfaces.

An analysis of the data (disinfection quality control) indicates that the proposed disinfectant in 2% and 3% concentration, used in a given mode of 500 ml / m ^{2 of the} treated area with an exposure of 1.5-2 hours, ensures the complete destruction of microorganisms at all livestock facilities.

It should be noted that even with poor cleaning of the premises for keeping animals, the treatment with the drug was effective in 100% of cases.

Thus, production tests of a new disinfectant have shown the potential for use as the most effective for disinfection of livestock facilities.

The use of a new disinfectant ensures the achievement of the purpose of the invention: increasing the disinfecting effect, which allows us to conclude that the claimed solution meets the criterion of "positive effect".

The set of distinctive features meets the criteria of "novelty" and "inventive step".

Claims (1)

A disinfectant including glutaraldehyde, glyoxal, alkyldimethylbenzylammonium chloride and water, characterized in that it further comprises alkyldimethylethylbenzylammonium chloride and anionic surfactant in the following ratio, wt.%:
glutaraldehyde 3.0-4.0 glyoxal 2.5-2.8 alkyldimethylbenzylammonium chloride 3.0-4.0 Alkyldimethylethylbenzylammonium chloride 4.0-5.0 anionic surfactant 8.0-9.0 water up to 1 l