RU2437663C2 - Method of correcting intestinal microflora - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to medicine, in particular, to field of intestine disbiosis, and can be applied for prevention or within complex therapy. Method of correcting intestinal microflora includes per oral introduction of prebiotic medication, and as prebiotic medication applied is mannose monopreparation in amount 0.01-0.02% to weight of experimental animal bode, and it is introduced in food diet of experimental animals until normal microflora is restored.

EFFECT: claimed method of intestinal microflora correction makes it possible to restore intestinal microflora, excluding risk of allergic reactions development due to application of mannose monopreparation.

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Description

The invention relates to medicine, in particular to the field of correction of intestinal dysbiosis, and can be used for prevention or as part of complex therapy.

It is known that intestinal dysbiosis (dysbiosis) is a qualitative and / or quantitative change in the composition of normal intestinal microflora (intestinal biocenosis). Currently, according to the Russian Academy of Sciences, 90% of the population have certain manifestations of dysbiosis, therefore, of course, the search and study of substances exhibiting prebiotic activity and participating in the restoration of normal flora of organisms is relevant. Prebiotics are indigestible food ingredients that have beneficial effects on health. Substances classified as prebiotics must meet the following criteria: do not hydrolyze and absorb in the upper gastrointestinal tract, selectively ferment with one or more types of potentially useful bacteria that are commensals and live in the colon, and improve the microflora of the colon. An important property of prebiotics is their ability to penetrate into the distal colon, where a number of chronic diseases can develop. It is assumed that the microflora of this intestine plays an important role in the development or suppression of this pathology. Dietary carbohydrates are the main fermentable substrate in the large intestine and are utilized by fermentation by bacteria [A.I. Khavkin, S.V. Belmer, N.S. Zhikhareva. Dietary fiber in the correction of microecological disorders in children. Therapist. 2002, No. 6].

Normalization of intestinal microflora can be achieved in two ways:

- by the method of colonizing the intestines with missing microorganisms. This approach is based on therapy using various kinds of live bacterial cultures (bifidumbacterin, lactobacterin, etc.);

- a method based on the introduction of prebiotics - substances that contribute to the activation of growth and vital activity of their own beneficial flora.

The closest in technical essence and the achieved effect is a method of correcting the microflora of the gastrointestinal tract [patent RU No. 23232251 C2, publ. in bull. No. 11 dated April 20, 2008, Nekrasova VB, Aleksandrovich N.Zh.], providing for the combined use of the probiotic product of the liquid concentrate of the symbiotic fermented milk product "NARINE-FORTE" and the prebiotic product of the syrup "Fitolon" with chlorophyll, which are taken during or after eating.

The disadvantage of this method is the high risk of allergic reactions due to the multicomponent composition of the drug, the complexity of dosing of drugs.

The technical task is to develop a method for the correction of intestinal microflora, which allows to restore the intestinal microflora through the use of mannose prebiotic, to eliminate the risk of allergic reactions due to the use of a single drug, to simplify the method of using the drug.

To solve the technical problem of the invention, a method for correcting intestinal microflora is proposed, which involves the oral administration of a prebiotic preparation, in which it is new that mannose is used as a prebiotic preparation in an amount of 0.01-0.02% of the body weight of an experimental animal, and it in the diet of experimental animals to restore normal flora.

The technical result consists in the restoration of intestinal microflora through the use of mannose prebiotic, in eliminating the risk of allergic reactions due to the use of a single drug, in simplifying the methodology of using the drug.

The method of correction of intestinal microflora is as follows: the normoflora of experimental animals is determined, then an antibiotic is administered orally, for example, doxycycline hydrochloride produced by Belmedpreparaty OJSC, achieving development of dysbiosis in experimental animals, the microflora of animals with dysbiosis is determined, and then mannose monopreparation is introduced into the diet of experimental animals in an amount of 0.01-0.02% of the body weight of the experimental animal. The control is carried out for normoflora of experimental animals until its full recovery.

The method is illustrated by the following examples.

Example 1

The method was tested on white outbred mice. For research, the normoflora of experimental animals obtained from the nursery was determined. Then, the antibiotic doxycycline hydrochloride produced by JSC Belmedpreparaty at a dose of 10 mg was orally administered to laboratory animals at a dose of 10 mg for 7 days. As a result, the elimination of normal microflora was observed and the intestines of animals were contaminated with conditionally pathogenic microflora (dysbiosis).

Regarding the most important representatives of normoflora after the introduction of the antibiotic, the following was established: the average amount of E. coli in feces increased to 10 ⁸ CFU / ml, Bifidobacterium spp. not sown at all, and the content of Lactobacillus spp. reduced to 10 ³ CFU / ml.

In addition, significant numbers of enterococci were found in the intestines of experimental animals, which are not found in normal animals.

Then, these animals were fed mannose in the amount of 1 mg (0.01% of the body weight of the experimental animal) into the feed until normal flora was restored. Microflora was monitored every 5 days. The results of the analyzes are presented in table 1.

Example 2

The method of correction of intestinal microflora was carried out analogously to example 1, but mannose was introduced into the feed composition in an amount of 2 mg (0.02% of the body weight of the experimental animal). The results are presented in table 1.

As can be seen from table 1, the use of mannose in an amount of 0.01-0.02% of the body weight of the experimental animal allows you to restore the intestinal flora.

The introduction into the diet of experimental animals of mannose in an amount of less than 0.01% of the body weight of the experimental animal required a long time to restore normal flora, and the introduction of mannose in an amount of more than 0.02% of the body weight of the experimental animal is not economically feasible.

Mannose has a prebiotic effect and helps to restore the composition and abundance of normoflora of experimental animals.

The proposed method for the correction of intestinal microflora allows you to:

- restore the intestinal microflora through the use of mannose prebiotic,

- eliminate the risk of allergic reactions due to the use of a single drug,

- simplify the use of the drug.

Table 1 Microbiocenosis of experimental animals Microorganism
we Quantification of microorganisms in samples (CFU / g feces) microflora of animals from the nursery (normoflora) microflora of animals with dysbiosis the microflora of animals after the introduction of mannose into the diet over time, days 5 10 fifteen dosage of 0.01% of body weight E. coli 10 ⁵ -10 ⁶ 10 ⁸ 10 ⁷ 10 ⁷ Bifidobacterium spp. 10 ³ are absent 10 ³ 10 ³ Lactobacillus spp. 10 ¹⁰ 10 ³ 10 ⁷ 10 ¹¹ dosage of 0.02% of body weight E.coli 10 ⁵ -10 ⁶ 10 ⁸ 10 ⁷ 10 ⁸ 10 ⁷ Bifidobacterium spp. 10 ³ are absent 10 ³ 10 ³ 10 ³ Lactobacillus spp. 10 ¹⁰ 10 ³ 10 ¹⁰ 10 ¹¹ 10 ¹¹

Claims (1)

A method for the correction of intestinal microflora, which provides for the oral administration of a prebiotic preparation, characterized in that a mono preparation of mannose is used in the amount of 0.01-0.02% of the experimental animal’s body weight as a prebiotic preparation, and it is introduced into the animal’s diet until normal flora is restored.