RU2420736C1 - Method of filling eye tissue - Google Patents

Abstract

FIELD: physics.

SUBSTANCE: cut rectangular or square section of eye tissue is carefully straightened. Two opposite edges are matched end-to-end with the sclera outside and fastened with one stitch using an atraumatic needle with a 3/00-5/00 thread, forming a tissue pipe which is placed perpendicularly on the base. Filling is then carried out.

EFFECT: use of the disclosed method enables to maintain the natural proportion of eye tissue, having different density, at the filling phase and therefore ensuring quality microscopic examination.

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Description

The invention relates to medicine, namely to methods for the preparation of histological preparations.

There is a traditional method of filling the eye tissues that underwent total fixation after enucleation from the orbit. According to the method, the excised tissues of the eye are laid non-oriented on the base and filled with paraffin or celloidin [2]. Another method is designed to reduce shrinkage and deformation of the organ wall with the aim of fixing tissues by the value of tissue pressure and involves the reduction of artifacts and artifact changes [1].

However, in preparation for filling the eye tissues with different densities in a known, traditional way — the retina, choroid, sclera often shift relative to each other even at the stage of cutting and laying on the base and during pouring. There is a spatial disorientation of softer tissues (retina, choroid) relatively denser (sclera), namely: twisting, folding, tearing, etc., which are disadvantages. As a result, subsequent microtomy and histology do not differ in quality, which requires repeated preparation of the drug for histological examination.

The objective of the proposed method is to preserve the natural ratio of eye tissues having different densities at the stage of filling and subsequently ensure high-quality microscopic examination.

The task is achieved due to the fact that the cut area of the tissues of the eye is gently straightened, two opposite edges align the end to end with the sclera outward and fasten with one stitch using an atraumatic needle with a thread 3 / 00-5 / 00, forming a tissue tube that is installed perpendicular to base, then fill.

The drawing shows a diagram of a fabric tube prepared for filling, where: 1 - sclera, 2 - choroid, 3 - retina, base - 4, fastening suture - 5, formed tissue tube - 6.

The method is as follows. A rectangular or square section of the planned length and width is cut out from the fixed eyeball. The cut out section of the eye is laid by the sclera on a flat surface, the shells are neatly straightened, the opposite edges of the cut out section are compared end to end and fastened with a seam (5) with one stitch through all layers with an atraumatic needle with a thread 3 / 00-5 / 00. Thus, a short tube is formed, the sclera is located on the outside (1), the soft eye structures remain inside: the choroid (2), the retina (3). The formed tissue tube (6) is placed on the base (4), which is oriented perpendicular to it, and is filled in the standard way, forming a block from which the tissues are cut with a microtome in the transverse direction of the tube for subsequent laying on a glass slide.

Tissues located inside the tissue scleral tube (1) retain their natural orientation, do not move, do not twist and do not break, which ensures high-quality subsequent microscopic examination, including the ability to examine the entire preparation in one field of view. This is achieved due to the fact that the sclera (1) in the blocks prepared in this way serves as a skeleton that does not allow soft tissues: choroid (2), retina (3) to move during subsequent staining of sections.

The proposed method allows to preserve the natural ratio of the tissues of the eye and to conduct high-quality microscopic examination.

Bibliography

1. A.S. USSR No. 1638605, G01 No. 1/28, 12/01/1990

2. Romeis B. Microscopic technology. - Publishing House I.-L., - 1954. - 718 p.

Claims (1)

A method for filling eye tissues in the preparation of histological preparations, including laying on the base of the excised tissue section of the eye of a rectangular or square shape of the planned length and width, followed by pouring with paraffin or celloidin, characterized in that the edges of the excised tissue region of the eye are neatly straightened, two opposite edges align the end to the end of the sclera to the outside, fastened with one stitch using an atraumatic needle with a thread 3 / 00-5 / 00, forming a tube that is installed perpendicular to the base, ATEM produces fill.

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