RU2411289C2 - Pantoea genus bacteria, producer of l-aspartate or metabolite, which is derivative of l-aspartate, and method of producing l-aspartate or metabolite which is derivative of l-aspartate - Google Patents

Pantoea genus bacteria, producer of l-aspartate or metabolite, which is derivative of l-aspartate, and method of producing l-aspartate or metabolite which is derivative of l-aspartate Download PDF

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RU2411289C2
RU2411289C2 RU2008138615/10A RU2008138615A RU2411289C2 RU 2411289 C2 RU2411289 C2 RU 2411289C2 RU 2008138615/10 A RU2008138615/10 A RU 2008138615/10A RU 2008138615 A RU2008138615 A RU 2008138615A RU 2411289 C2 RU2411289 C2 RU 2411289C2
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aspartate
bacterium
dehydrogenase
gene encoding
glutamate
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Ольга Николаевна Мохова (RU)
Ольга Николаевна Мохова
Татьяна Михайловна Куваева (RU)
Татьяна Михайловна Куваева
Любовь Игоревна Голубева (RU)
Любовь Игоревна Голубева
Александра Викторовна Колоколова (RU)
Александра Викторовна Колоколова
Жанна Иосифовна Каташкина (RU)
Жанна Иосифовна Каташкина
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Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ)
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Abstract

FIELD: chemistry.
SUBSTANCE: invention relates to a method of producing L-aspartate or a metabolite which is a derivative of L-aspartate, using Pantoea genus bacteria which is modified in such a way that α-ketoglutarate dehydrogenase or citrate synthase activity in it is low; phosphoenolpyruvate carboxylase or pyruvate carboxylase and glutamate dehydrogenase or glutamate synthase activity is high. Said bacteria can additionally be modified in such a way that expression of a gene selected from the following group is low: a gene which codes asparate ammonia lyase (aspartase), pyruvate kinase, glucose dehydrogenase, malate dehydrogenase.
EFFECT: invention enables to obtain L-aspartate or its derivatives with high efficiency.
22 cl, 8 dwg, 12 tbl, 14 ex

Description

Текст описания приведен в факсимильном виде.

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Claims (22)

1. Бактерия, принадлежащая к роду Pantoea, - продуцент L-аспартата или метаболита, являющегося производным L-аспартата, отличающаяся тем, что указанная бактерия модифицирована таким образом, что в ней:
снижена активность α-кетоглутаратдегидрогеназы;
снижена активность цитратсинтазы;
повышена активность фосфоенолпируваткарбоксилазы или пируваткарбоксилазы и
повышена активность глутаматдегидрогеназы или глутаматсинтазы.1. A bacterium belonging to the genus Pantoea is a producer of L-aspartate or a metabolite derived from L-aspartate, characterized in that said bacterium is modified in such a way that it contains:
decreased activity of α-ketoglutarate dehydrogenase;
decreased activity of citrate synthase;
increased activity of phosphoenolpyruvate carboxylase or pyruvate carboxylase and
increased activity of glutamate dehydrogenase or glutamate synthase. 2. Бактерия по п.1, отличающаяся тем, что активность α-кетоглутаратдегидрогеназы и цитратсинтазы снижена путем ослабления экспрессии гена, кодирующего α-кетоглутаратдегидрогеназу и цитратсинтазу.2. The bacterium according to claim 1, characterized in that the activity of α-ketoglutarate dehydrogenase and citrate synthase is reduced by attenuating the expression of a gene encoding α-ketoglutarate dehydrogenase and citrate synthase. 3. Бактерия по п.2, отличающаяся тем, что указанная экспрессия гена, кодирующего α-кетоглутаратдегидрогеназу и цитратсинтазу, ослаблена путем инактивации гена, кодирующего указанный фермент, в хромосоме бактерии.3. The bacterium according to claim 2, characterized in that said expression of the gene encoding α-ketoglutarate dehydrogenase and citrate synthase is attenuated by inactivation of the gene encoding the specified enzyme in the bacterial chromosome. 4. Бактерия по п.1, отличающаяся тем, что активность фосфоенолпируваткарбоксилазы или пируваткарбоксилазы и активность глутаматдегидрогеназы или глутаматсинтазы увеличены путем усиления экспрессии гена, кодирующего указанные ферменты.4. The bacterium according to claim 1, characterized in that the activity of phosphoenolpyruvate carboxylase or pyruvate carboxylase and the activity of glutamate dehydrogenase or glutamate synthase are increased by enhancing the expression of a gene encoding these enzymes. 5. Бактерия по п.4, отличающаяся тем, что указанная экспрессия генов, кодирующих фосфоенолпируваткарбоксилазу или пируваткарбоксилазу и глутаматдегидрогеназу или глутаматсинтазу, усилена путем модификации последовательности, контролирующей экспрессию гена или путем увеличения числа копий гена.5. The bacterium according to claim 4, characterized in that the expression of genes encoding phosphoenolpyruvate carboxylase or pyruvate carboxylase and glutamate dehydrogenase or glutamate synthase is enhanced by modifying the sequence that controls gene expression or by increasing the number of copies of the gene. 6. Бактерия по п.4, отличающаяся тем, что гены, кодирующие фосфоенолпируваткарбоксилазу и глутаматдегидрогеназу, являются генами Escherichia coli.6. The bacterium according to claim 4, characterized in that the genes encoding phosphoenolpyruvate carboxylase and glutamate dehydrogenase are Escherichia coli genes. 7. Бактерия по п.4, отличающаяся тем, что ген, кодирующий глутаматдегидрогеназу, является геном, кодирующим НАД+-зависимую глутаматдегидрогеназу из Pantoea ananatis.7. The bacterium according to claim 4, characterized in that the gene encoding glutamate dehydrogenase is a gene encoding NAD + -dependent glutamate dehydrogenase from Pantoea ananatis. 8. Бактерия по п.4, отличающаяся тем, что ген, кодирующий пируваткарбоксилазу, является геном из Sinorhizobium meliloti.8. The bacterium according to claim 4, characterized in that the gene encoding pyruvate carboxylase is a gene from Sinorhizobium meliloti. 9. Бактерия по п.1, отличающаяся тем, что указанной бактерией является бактерия Pantoea ananatis.9. The bacterium according to claim 1, characterized in that said bacterium is the Pantoea ananatis bacterium. 10. Бактерия по п.1, отличающаяся тем, что метаболиты, являющиеся производным L-аспартата, включают L-треонин, L-лизин, L-метионин и L-гомосерин.10. The bacterium according to claim 1, characterized in that the metabolites derived from L-aspartate include L-threonine, L-lysine, L-methionine and L-homoserine. 11. Бактерия, принадлежащая к роду Pantoea, - продуцент L-аспартата или метаболита, являющегося производным L-аспартата, отличающаяся тем, что указанная бактерия модифицирована таким образом, что в ней:
снижена активность α-кетоглутаратдегидрогеназы;
снижена активность цитратсинтазы;
повышена активность фосфоенолпируваткарбоксилазы или пируваткарбоксилазы; и
повышена активность глутаматдегидрогеназы или глутаматсинтазы;
при этом указанная бактерия дополнительно модифицирована таким образом, что в ней ослаблена экспрессия гена, выбранного из группы, состоящей из:
гена, кодирующего аспартатаммонийлиазу (аспартазу);
гена, кодирующего пируваткиназу;
гена, кодирующего глюкозодегидрогеназу;
гена, кодирующего малатдегидрогеназу.11. A bacterium belonging to the genus Pantoea is a producer of L-aspartate or a metabolite derived from L-aspartate, characterized in that said bacterium is modified in such a way that it contains:
decreased activity of α-ketoglutarate dehydrogenase;
decreased activity of citrate synthase;
increased activity of phosphoenolpyruvate carboxylase or pyruvate carboxylase; and
increased activity of glutamate dehydrogenase or glutamate synthase;
while the specified bacterium is further modified in such a way that expression of a gene selected from the group consisting of:
a gene encoding aspartate ammonium lyase (aspartase);
a gene encoding pyruvate kinase;
a gene encoding glucose dehydrogenase;
gene encoding malate dehydrogenase. 12. Бактерия по п.11, отличающаяся тем, что активность α-кетоглутаратдегидрогеназы и цитратсинтазы снижена путем ослабления экспрессии гена, кодирующего α-кетоглутаратдегидрогеназу и цитратсинтазу.12. The bacterium according to claim 11, characterized in that the activity of α-ketoglutarate dehydrogenase and citrate synthase is reduced by attenuating the expression of a gene encoding α-ketoglutarate dehydrogenase and citrate synthase. 13. Бактерия по п.11, отличающаяся тем, что указанная экспрессия гена, кодирующего α-кетоглутаратдегидрогеназу, цитратсинтазу, аспартатаммонийлиазу (аспартазу), пируваткиназу, глюкозодегидрогеназу или малатдегидрогеназу, ослаблена путем инактивации гена, кодирующего указанный фермент, в хромосоме бактерии.13. The bacterium according to claim 11, characterized in that said expression of a gene encoding α-ketoglutarate dehydrogenase, citrate synthase, aspartate ammonium lyase (aspartase), pyruvate kinase, glucose dehydrogenase or malate dehydrogenase is attenuated by the inactivation of the enzyme chromogen, code. 14. Бактерия по п.12, отличающаяся тем, что активность фосфоенолпируваткарбоксилазы или пируваткарбоксилазы и активность глутаматдегидрогеназы или глутаматсинтазы увеличены путем усиления экспрессии гена, кодирующего указанные ферменты.14. The bacterium according to claim 12, characterized in that the activity of phosphoenolpyruvate carboxylase or pyruvate carboxylase and the activity of glutamate dehydrogenase or glutamate synthase are increased by enhancing the expression of a gene encoding these enzymes. 15. Бактерия по п.14, отличающаяся тем, что указанная экспрессия генов, кодирующих фосфоенолпируваткарбоксилазу или пируваткарбоксилазу и глутаматдегидрогеназу или глутаматсинтазу, усилена путем модификации последовательности, контролирующей экспрессию гена или путем увеличения числа копий гена.15. The bacterium of claim 14, wherein said expression of genes encoding phosphoenolpyruvate carboxylase or pyruvate carboxylase and glutamate dehydrogenase or glutamate synthase is enhanced by modifying the sequence that controls gene expression or by increasing the number of copies of the gene. 16. Бактерия по п.14, отличающаяся тем, что гены, кодирующие фосфоенолпируваткарбоксилазу и глутаматдегидрогеназу, являются генами Escherichia coli.16. The bacterium according to 14, characterized in that the genes encoding phosphoenolpyruvate carboxylase and glutamate dehydrogenase are Escherichia coli genes. 17. Бактерия по п.14, отличающаяся тем, что ген, кодирующий глутаматдегидрогеназу, является геном, кодирующим НАД+-зависимую глутаматдегидрогеназу из Pantoea ananatis.17. The bacterium of claim 14, wherein the gene encoding glutamate dehydrogenase is a gene encoding NAD + -dependent glutamate dehydrogenase from Pantoea ananatis. 18. Бактерия по п.14, отличающаяся тем, что ген, кодирующий пируваткабоксилазу, является геном из Sinorhizobium meliloti.18. The bacterium of claim 14, wherein the gene encoding pyruvate carboxylase is a gene from Sinorhizobium meliloti. 19. Бактерия по п.11, отличающаяся тем, что указанной бактерией является бактерия Pantoea ananatis.19. The bacterium according to claim 11, characterized in that said bacterium is the Pantoea ananatis bacterium. 20. Бактерия по п.11, отличающаяся тем, что метаболиты, являющиеся производным L-аспартата, включают L-треонин, L-лизин, L-метионин и L-гомосерин.20. The bacterium according to claim 11, characterized in that the metabolites derived from L-aspartate include L-threonine, L-lysine, L-methionine and L-homoserine. 21. Способ получения L-аспартата или метаболита, являющегося производным L-аспартата, включающий:
выращивание бактерии по любому из пп.1-20 в питательной среде и
выделение указанного L-аспартата из культуральной жидкости.21. A method for producing L-aspartate or a metabolite derived from L-aspartate, comprising:
growing bacteria according to any one of claims 1 to 20 in a nutrient medium and
the allocation of the specified L-aspartate from the culture fluid. 22. Способ по п.21, отличающийся тем, что метаболиты, являющиеся производным L-аспартата, включают L-треонин, L-лизин, L-метионин и L-гомосерин. 22. The method according to item 21, wherein the metabolites derived from L-aspartate include L-threonine, L-lysine, L-methionine and L-homoserine.
RU2008138615/10A 2008-09-30 2008-09-30 Pantoea genus bacteria, producer of l-aspartate or metabolite, which is derivative of l-aspartate, and method of producing l-aspartate or metabolite which is derivative of l-aspartate RU2411289C2 (en)

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RU2008138615/10A RU2411289C2 (en) 2008-09-30 2008-09-30 Pantoea genus bacteria, producer of l-aspartate or metabolite, which is derivative of l-aspartate, and method of producing l-aspartate or metabolite which is derivative of l-aspartate
PCT/JP2009/067449 WO2010038905A1 (en) 2008-09-30 2009-09-30 A BACTERIUM BELONGING TO THE GENUS Pantoea PRODUCING AN L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES AND A METHOD FOR PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES

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