RU2410429C2 - Method of acting on biological objects - Google Patents

Abstract

FIELD: chemistry; biochemistry.

SUBSTANCE: invention relates to biotechnology and specifically to biophysical methods of acting on biological objects. The method is realised with a single pulse in a vector potential field, as well as with a single pulse and/or packet of separate pulses in a magnetic vector potential field with field strength of 0.2-500 mT. Duration of the single pulses is in the interval of 1·10^-6-0.6·10² s, the pulse rise duration is in the interval of 0.0001-0.5 times the pulse duration and the pulse drop duration is in the interval of 0.001-15 times the pulse duration.

EFFECT: invention can be used in different fields of engineering to stimulate growth of biological objects.

7 cl, 4 tbl, 4 ex

Description

The invention relates to the field of biotechnology, and in particular to the field of biophysical methods of influencing biological objects, and can be applied in various fields of technology to activate the growth of plants and microorganisms.

A known method of exposure to a biological object with a magnetic field [RU, US Pat. 2089242], characterizing the effect of a magnetic field created by a single-crystal magnetic film, and the object of exposure is brought into direct contact with the specified single-crystal magnetic film. The disadvantages of the known solution should recognize the need to bring the processing object into direct contact with a single-crystal magnetic film, since in addition to the magnetic field, the object is additionally affected by hydrophobic and hydrophilic factors, which depend not only on the composition of the single-crystal magnetic film, but also on the quality and technology of cleaning it before the test.

Known methods of exposure to biological fluids [RU, US Pat. 2148646]. It is suggested that before contacting the water and / or the water system with the bioobject, the water and / or water system is treated with a magnetodomain film structure. The ferritic garnet structure is preferably used. The processing time of water and / or the water system depends on the type of object being processed and is selected experimentally.

It is preferable to place the vessel with water and / or the water system on the magnetodomain film structure. A disadvantage of the known solution should be recognized as the short time of maintaining increased activity of water subjected to such magnetic treatment, and sufficient irreproducibility of the results.

The closest analogue is recognized as a method of exposing a biological object to a magnetic field created by a magnetic film using a magnetodomain film having a saturation magnetization of 5-50 mT at a domain wall density of 30-280 inverse millimeters located at a distance of 1-100 mm from a sample of biological medium placed in a sealed container [RU, US Pat. 2142012 (13)]. The disadvantages of the known technical solutions are the lack of reproducibility of the results and low processing efficiency of biological objects.

The technical problem solved by the use of the present invention is to increase the reproducibility of the results of exposure and its effectiveness, which makes its results sufficiently reliable and suitable for use in various fields of technology, including to stimulate plant growth.

To achieve the specified technical result on biological objects, contactless action is carried out with a single pulse of the vector potential field, while a single pulse of the magnetic potential field is applied with a field strength in the range of 0.2-500 mT, while a single pulse is selected with a duration in the range of 1 · 10 ^-6 -0.6 · 10 ² sec, while the duration of the decline of the pulse is selected in the range (0.001-15) of the pulse duration; biological objects are affected by a packet of single pulses of a vector potential field, while biological objects are affected by a packet of single pulses of a magnetic vector potential field with a duration of 10 ^-6 -0.6 · 10 ² sec, the pulse decay duration is selected in the range (0.001-15) pulse duration, pulse rise time is selected in the range (0.0001-0.5) of the pulse duration, the pulse repetition rate in the packet is selected in the range of 10 ^-2 -2 · 10 ³ Hz, and the field strength is selected in the range of 0.2-500 mTl; the biological object is first affected by a single pulse of the vector potential field, then by a packet of single pulses of the vector potential field, while the biological object is first affected by a single pulse of the magnetic vector potential field, then by the packet of single pulses of the magnetic vector potential field, the pulse duration being selected in the range of 10 ^-6 -0.6 × 10 ² sec fall Time is selected in the range (0.001-15) pulse width, pulse rise duration selected intervals e (0,0001-0,5) the pulse duration, the pulse repetition frequency in the package is selected in the range of (10 ^-2 -2 10 ³⁾ Hz, the time interval between a single pulse and pulse packet is selected in the range of 10 ^{- 2} -1.8 · 10 ³ sec, and the field strength is selected in the range of 0.2-500 mT;

the biological object is placed in an electric field and exposed to it with a single pulse and (or) a packet of single pulses of the magnetic vector potential field, the pulse duration being selected in the range of 10 ^-6 -0.6 · 10 ² sec, the pulse decay duration was selected in the interval ( 0.001-15) pulse duration, pulse rise time is selected in the interval (0.0001-0.5) pulse duration, and the pulse repetition rate in the packet is selected in the range (10 ^-2 -2 · 10 ³ ) Hz, the time interval between a single pulse and pulse burst select n in the range 10 ^-2 -1.8 · 10 ³ sec, the field strength is selected in the range of 0,2-500 mT, while the electric field is selected in the range of 10 ¹ to 10 ⁴ V / cm.

The invention can be illustrated by the following examples.

Example 1

Dry radish seeds (grade 18 days, GOST 28676.6-90, batch 190) are placed in a container made of dielectric material, which is installed in the magnetic field potential field device (Pat. RF No. 51783, S.V. Mashnin, A.S. Machine). A container with dry radish seeds is affected by single pulses: batch No. 1 - one pulse; party number 2 - two pulses; party number 3 - three pulses; party number 4 - four pulses; party number 5 - five pulses; party number 6 - six pulses; party number 7 - seven pulses; party number 8 - eight pulses; party number 9 - nine pulses; party number 10 - ten pulses.

After exposure, the seeds were germinated at room temperature in Petri dishes using ordinary tap water with a total salinity of 120-210 mg / L, iron content 0.42 mg / L, copper 0.02 mg / L.

The growth rate of seedlings was controlled: Vav (mm per hour) and That is the time of the beginning of emergence of the seedling (hour). In each sample (batch) there were 30 seeds. Table 1 shows the data for radish seeds - cultivar “18 days” (batch 190): duration of a single pulse of 800 μs, duration of rise of a pulse of 10 μs, duration of a pulse decline of 100 μs, field strength of 3.6 mT. The pulse repetition rate was 1 Hz.

Table number 1 N (parties) That hour Vcp, mm / hour

one 35 0.19 2 35 0,07 3 54 0.14 four 90 0,07 5 71 0.12 6 73 0.14 7 51 0.18 8 115 0,07 9 125 0.09 10 43 0.13

In the control: Vcp = 0.06 mm / hour, That = 84 hours.

Similarly, higher seedling growth rates are obtained, with the number of germinated seeds reaching 95-98%.

The specific modes of the effect of the pulses of the magnetic vector potential field on the seeds of various plants (dry or wet) are selected experimentally.

Example 2

Dry radish seeds - variety "18 days", batch 190. Field strength 0.2 mT, single pulse duration - 3.5 μs, pulse rise time 0.1 μs, pulse fall time 0.35 μs, single pulse repetition rate ( from 2 to 10 pulses) - 1 Hz: parties No. 1-10 - data from table No. 2. Everything else is the same as in example 1.

Table number 2 N (parties) That hour Vcp, mm / hour one thirty 0.09 2 45 0.06 3 one hundred 0.09 four - - 5 48 0.12 6 48 0.12 7 80 0.11 8 81 0.11 9 - - 10 thirty 0.09 Note: “-” means no growth within 250 hours of observation.

In the control: Vcp = 0.06 mm / hour, That = 83 hours, the number of germinated seeds 72%.

Similarly, higher seedling growth rates are obtained, with the number of sprouted seeds reaching 92-96%.

The specific modes of the effect of the magnetic field potential field pulse on the seeds of various plants (dry or wet) are selected experimentally.

Example 3

Dry radish seeds - variety “18 days”, batch 190. Field strength 1.8 mT, single pulse duration 3.5 μs, pulse rise time 0.1 μs, pulse fall time 1 μs, single pulse repetition rate (from 2 to 10 pulses) - 1 Hz: parties No. 1-10 - data from table 3. Everything else is the same as in example 1.

Table number 3 N (parties) That hour Vcp, mm / hour one fifty 0.18 2 thirty 0.18 3 52 0.18 four 67 0.20 5 58 0.28 6 85 0.09 7 84 0.09 8 - - 9 - - 10 55 0.09 Note: “-” means no growth within 250 hours of observation.

In the control: That = 83 hours, Vcp = 0.05 mm / hour, the number of sprouted seeds - 65%.

Similarly, higher seedling growth rates are obtained, with the number of germinated seeds reaching 96-99%.

The specific modes of the effect of the magnetic field potential field pulse on the seeds of various plants (dry or wet) are selected experimentally.

Example 4

Dry radish seeds - variety “18 days”, batch 190. Field strength 3.6 mT, single pulse duration 3.5 μs, pulse rise time 0.18 μs, pulse decline duration 0.7 μs, single pulse repetition rate (from 2 to 10 pulses) - 1 Hz: parties No. 1-No. 10 - data from table 4. Everything else is the same as in example 1.

Table number 4 N (parties) That hour Vcp, mm / hour one 47 0.23 2 46 0.08 3 45 0.08 four - - 5 - - 6 72 0,07 7 73 0,07 8 76 0,07 9 - - 10 - - Note: “-” means no growth within 250 hours of observation.

In the control: That = 81 hours, Vcp = 0.06 mm / hour, the number of sprouted seeds - 72%.

Similarly, higher seedling growth rates are obtained, with the number of sprouted seeds reaching 96-99%.

The specific modes of the impact of the pulse (s) of the vector potential field on the seeds of various plants (dry or wet) are selected during the experiment.

Using the invention will allow, for example, to increase the growth rate and germination of seeds of various plants.

Claims (7)

1. A method of influencing a biological object, including the non-contact effect of a physical factor, characterized in that, in order to stimulate (regulate) the development of the biological object, the biological object is affected by a single pulse and / or packet of pulses of the vector potential field, the field strength being selected in range 0.2-500 mT, pulse duration selected in the range 1 · 10 ⁶ -0.6 · 10 ² s, pulse decay duration selected in the range 0.001-15 pulse duration, pulse repetition rate in the packet selected a wound in the range of 0.01-2000 Hz. 2. The method according to claim 1, characterized in that the pulse rise time is selected in the range of 0.0001-0.5 pulse duration. 3. The method according to claim 2, characterized in that the time interval between a single pulse and a pulse packet is selected in the range of 0.01-1800 s. 4. The method according to claim 3, characterized in that the biological object is placed in an electric field and exposed to it with a single and / or packet of single pulses of a vector potential field. 5. The method according to claim 4, characterized in that the pulse duration is selected in the range of 1 · 10 ^-6 -0.6 · 10 ² s, the pulse decay duration is selected in the range of 0.001-15 pulse duration, the pulse repetition rate in the packet is selected in in the range of 0.01-2000 Hz, the time interval between a single pulse and a packet of pulses is selected in the range of 0.01-1800 s, while the field strength of the vector potential is selected in the range of 0.2-500 mT. 6. The method according to claim 5, characterized in that the electric field is selected in the range of 10-10000 V / cm. 7. The method according to claim 6, characterized in that the pulse rise time is selected in the range of 0.0001-0.5 pulse duration.