RU2395229C1 - Method of hyperparathyreosis express-diagnostics - Google Patents

Abstract

FIELD: medicine. ^ SUBSTANCE: invention relates to medicine, endocrinology, biology, physiology. For express-diagnostics of hyperparathyreosis in zones of parathyroid glands projection locally in region above upper and lower poles of parathyroid glands symmetrically placed are four glass plates onto which 1% solution of aspartic amino acid in amount 0.01-0.03 ml on each plate is preliminarily applied in form of path. Plates are kept for 3-5 minutes, after that dried in thermostat at T=+18-20C during 2-3 minutes. After that they are examined in polarised light with quartz compensator. If crystals-aggregations from radial spherulites are present in preparations hyperparathyreosis is diagnosed. ^ EFFECT: method is simple in application, economical, increases self-descriptiveness of hyperparathyreosis diagnostics. ^ 9 dwg, 5 ex

Description

The invention relates to medicine, namely to endocrinology, biology, physiology, and can be used to diagnose hyperfunction of the parathyroid glands - hyperparathyroidism (HPT).

The parathyroid glands are the main regulators of the exchange of calcium and phosphorus, carried out using the hormone parathyroid hormone (PTH) synthesized by them.

The main targets of PTH are bones, kidneys, its effect on the absorption of calcium in the intestine is known. The glands are located above the upper and lower poles of the thyroid gland.

HPT occurs with gland adenoma and gland hyperplasia, their carcinoma, multiple endocrine neoplasia of types I and II, which is accompanied by overproduction of PTH (M.I. Balabolkin. Endocrinology. M., 1998; Dedov II, Melnichenko G.A. Fadeev V.V. Endocrinology, M., 2007).

Clinic GPT is diverse and its diagnosis is extremely difficult. Variegated Disease Syndromes, depending on the point of application of PTH, option: bone, gastrointestinal, urological neuropsychic, dental, cardiovascular. Known methods for determining parathyroid hormone: scintigraphy of the parathyroid glands using a radioactive pharmaceutical (RFP) and the immunochemiluminescent method "Sandwich" (Diagnostic imaging nuclear medicine; Advia Centaur XP System (USA, Bayer).

ANALOGUE - Stratigraphy of the parathyroid glands using radioactive technetium-RFP, activity 99 mc MIBI, 250-550 MBK (Diagnostic imaging nuclear medicine. Authors: Morton. Clark. Blodgelf. Christensen. O'Malley Sverens. Chinu. Woxman. Nance homson. Droten. Heston. 7 section 12 pp. Drawings-Copyrighf 2007 Aminsys Yns.).

The method of scintigraphy of the parathyroid glands of the radiopharmaceutical is based on the additional absorption by the mitochondria of the adenoma of the parathyroid glands of 99 m MIBI, and its delayed leaching from the adenoma compared to the surrounding thyroid tissue. Using this method allows to identify parathyroid adenoma in 85-90%.

The study consists of 2 stages.

The drug is administered 99 m Tc MIBI, 250-50 MBq.

1. Thyroid phase - after 30 minutes. after the introduction of 99 m Those MIBI, the drug is included in the thyroid gland, parathyroid glands, salivary glands, myocardium.

2. The hyperparathyroid phase, delayed, after 2 hours (normally after 2 hours, the radiopharmaceutical is completely released from the healthy glands).

3. Recording results using a technical device for recording scintigrams.

The disadvantages of the method.

1. The use of a radioactive drug.

2. The radiation load on the human body.

3. The use of an expensive technical device.

NEAREST ANALOGUE - Immunochemiluminescent Method “Sandwich” (Advia Centaur XP System (USA, Bayer company).

The method is based on the interaction of an antigen (analyte in a sample) and an antibody labeled with acridine ether (AE). During this reaction, energy is released in the form of light, the amount of which depends on the amount of analyte in the sample.

1. 200 μl of serum is placed in a cuvette.

2. Add 50 μl of light reagent containing antibodies labeled with acridine ether. Antibodies labeled with acridine ester bind only to the analyte-specific antigen in the sample.

3. Next, a solid phase is added containing paramagnetic particles coated with antibodies specific for the antigen in the sample. The cuvette is incubated at a temperature of - 37.0 ° C (2.5 min). Paramagnetic particles (PMPs) bind to antigens that are already associated with antibodies labeled with AE.

4. Carry out washing and aspiration (the cuvette enters the magnetic field, which attracts paramagnetic particles by a magnet. While it holds the paramagnetic particles in place, the material that does not contact them is washed out of the cuvette.

5. Add 300 μl of acid and alkali, the initiation of the chemiluminescent reaction. A chemiluminometer measures light emission in relative light units. When using the "Sandwich" method, the analyte concentration is proportional to the glow.

The disadvantages of the method

1. The use of human serum.

2. The use of expensive reagents.

3. The use of an expensive system - an automated immunological analyzer.

4. Automatic execution of the results, which may give a "failure".

The objectives of the invention

1. Providing rapid diagnosis of hyperfunction of the parathyroid glands producing parathyroid hormone.

2. Improving the information content of diagnostics.

3. Lower material and technical costs.

In the development of the present invention, the possibility of registering the biological radiation of the parathyroid glands with the help of a 1% aqueous solution of aspartic amino acid (AA) is used.

Biological informational radiation from various objects (living, biocasic, inert) is one of the general principles of energy-informational interaction in nature (V.I. Vernadsky. Chemical structure of the Earth's biosphere and its environment. M., 1965; V.P.Kaznacheev, L. P. Mikhailova, Bioinformation functions of natural electromagnetic fields (Novosibirsk, 1985).

Currently, science is actively developing the principles of information-wave biology and medicine. Under the influence of the front of the information wave radiation of the studied object, amino acids, changing their structure, thereby transmit information about the structure of the radiation source, as a result, a “mirror” reflection of the studied object is formed in it (I.V. Prangishvili. P.P. Garyaev, G. G. Tertyshny et al. Three-dimensional model of the processes of endogenous holographic control of the development of the spatial structure of biosystems. M., 2004; L.V. Savina. Energy-information interactions. Krasnodar, 2008).

The technical novelty of the proposal is a physical method for studying the function of the parathyroid glands - a state of hyperparathyroidism, characterized in that four glass plates are placed symmetrically in the areas of the projection of the parathyroid glands locally in the area above the upper and lower poles of the thyroid gland, onto which AA - 1% water is applied in the form of a path a solution of aspartic amino acid, with a volume of 0.01-0.03 ml per each, the plates are kept for 3-5 minutes, then the preparation is dried in a thermostat at T = + 18-20 ° C for 2-3 minutes, then they are examined under a microscope in polarized light with a quartz compensator and, if there are crystals in the preparations — aggregations from radial-ray spherulites, hyperparathyroidism is diagnosed.

The advantages of the proposed method

- To diagnose hyperfunction of the parathyroid glands, a 1% aqueous solution of aspartic amino acid (AA) was used, which records the biological radiation of the glands.

- The method provides high selective information content, diagnostic efficiency. The physiological and native methods are observed.

Compared with the basic methods for diagnosing hyperparathyroidism, the study time is reduced by 70%, and material costs - by 88-90%.

The method is as follows.

1. Prepare a 1% aqueous solution of AA.

2. A tare pipette AA is applied to four glass plates in the form of a thin film-track, with a volume of 0.01-0.03 ml each.

3. Glass plates with AA deposited on them are symmetrically placed in the projection zones of the parathyroid glands locally in the area above the upper and lower poles of the thyroid gland, incubated for 3-5 minutes.

4. The preparations are dried in a thermostat at T = + 18-20 ° C for 2-3 minutes.

5. Samples are examined in polarized light with a quartz compensator.

If there are crystals in the preparations - aggregations from radial beam spherulites, hyperparathyroidism is diagnosed.

The structure of a 1% aqueous solution AA, figure 1, there are crystals of predominantly rhombic form.

Figure 2 shows the histological preparation of the structure of the parathyroid gland of a healthy person (V.V. Almazov, L.S. Sutulov. Atlas on histology and embryology. M., 1978).

Figure 3 presents a typical structure of AA obtained by recording information from the hormone of the parathyroid glands - parathyroidin. For this purpose, AA is applied to the glass plate and surrounded on the periphery with a roller of parathyroidin hormone, kept in it for 3-5 minutes, then dried at T = + 18-20 ° C for 2-3 minutes, then examined in polarized light with quartz compensator (QC). See the patent of the invention "Method for the rapid identification of pharmaceutical and parapharmaceuticals introduced into the human body." Authors: Savina L.V., Kuznetsova E.A. No. 2271001. M. 2006. Figure 3 presents crystals in the form of radial beam spherulites.

Figure 4 shows typical AA structures obtained by recording the biological radiation of the parathyroid glands of a healthy person, for which four glass plates coated with AA were placed in the projection zones of the glands - above the upper and lower poles of the thyroid gland. There are single crystals in the form of radial beam spherulites. Conducted 189 clinical trials.

We give examples.

Example 1, Fig. 5. B. A., medical history (IB) No. 789. Diagnosis (Ds): Hyperparathyroidism. Multiple adenomatosis of the parathyroid glands.

Figure 5 presents crystals in the form of aggregations from radial beam spherulites.

Technology. 0.01 ml of AA in the form of a track was applied to each of the four glass plates, placed in the projection zones of the parathyroid glands (thyroid gland) locally symmetrically in the area above the upper and lower poles of the thyroid gland (thyroid gland), they were kept for 3 minutes. The plates were dried at T = + 18 ° C for 2 min, then examined in polarized light with CC. Aggregation crystals from radial beam spherulites are visible.

At the same time, the concentration of parathyroid hormone (PG) was determined in blood serum (SC), which turned out to be increased and amounted to 34 pmol / L (normal - 4.5 ± 1.1 pmol / L). The diagnosis of hyperparathyroidism was confirmed.

Example 2, Fig.6. B.W. Ds: Hyperparathyroidism. Solitary adenoma of the parathyroid gland. IS No. 1825. Figure 6 shows crystals of aggregation from radial beam spherulites.

Technology. On each of the four glass plates, 0.02 ml of AA was applied in the form of a track and placed in the area of the thyroid gland projection locally symmetrically in the region above the upper and lower poles of the thyroid gland, kept in them for 4 min, dried at T = + 19 ° C for 3 min and investigated in polarized light with CC. Aggregation crystals from radial beam spherulites are present.

At the same time, the level of GHG was determined in the SC, which turned out to be increased - 16.2 pmol / L. The diagnosis of hyperparathyroidism was confirmed.

Example 3, Fig.7. B.S. Ds: Hyperparathyroidism. Hyperplasia of the parathyroid glands. IS No. 243. 7 shows crystals of aggregation from radial beam spherulites.

Technology. On each of the four glass plates, 0.03 ml of AA was applied in the form of a track and placed in the projection area of the thyroid gland locally symmetrically in the area above the upper and lower poles of the thyroid gland, kept in them for 5 minutes, dried at T = + 20 ° C for 3 min and investigated in polarized light with CC. Aggregation crystals from radial-ray spherulites were found in the preparations.

At the same time, the level of GHG was determined in the SC, which turned out to be elevated and amounted to 15 pmol / L. The diagnosis of hyperparathyroidism was confirmed.

Example 4, Fig. 8. B. I. Ds: Hyperparathyroidism. Cancer of the parathyroid glands. IS No. 718. On Fig there are aggregations of radial beam spherulites.

Technology. On each of the four glass plates, 0.02 ml of AA was applied in the form of a track and placed in the projection area of the thyroid gland locally symmetrically in the region above the upper and lower poles of the thyroid gland, kept for 4 minutes, dried at T = + 19 ° C for 3 minutes, investigated in polarized light with QC. Aggregations from radial beam spherulites are present.

At the same time, the content of GHG was determined in the SC, which turned out to be increased and amounted to 18.8 pmol / L. The diagnosis of hyperparathyroidism was confirmed.

Example 5, Fig.9. B. B. Ds: Hyperparathyroidism. Parathyroid carcinoma. IS No. 389. Figure 9 shows aggregation crystals from radial beam spherulites.

Technology. On each of the four glass plates, 0.03 ml of AA was applied in the form of a track and placed in the projection zones of the thyroid gland over the upper and lower poles of the thyroid gland, held for 3 minutes, dried at T = + 18 ° C for 2 minutes. The preparations were examined under a polarization microscope with QA. Aggregations from radial beam spherulites are found.

At the same time, the level of GHG was determined in the SC, which turned out to be increased and amounted to 21.8 pmol / L. The diagnosis of hyperparathyroidism was confirmed.

Claims (1)

A method for express diagnostics of hyperparathyroidism, including a physical research method, characterized in that four glass plates are placed symmetrically in the areas of the projection of the parathyroid glands locally in the area above the upper and lower poles of the thyroid gland, onto which a 1% aqueous solution of aspartic is applied in the form of a track amino acids with a volume of 0.01-0.03 ml per plate, the plates are held for 3-5 minutes, the preparations are dried in a thermostat at T = 18-20 ° C for 2-3 minutes, then examined in polarized light with quartz hyperparathyroidism is diagnosed by a compensator and in the presence of aggregation crystals from radial beam spherulites in the preparations.

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