RU2385347C2 - Molecules of nucleic acids, which code wrinkled 1-like polypeptides and methods of their application in plants - Google Patents

Molecules of nucleic acids, which code wrinkled 1-like polypeptides and methods of their application in plants Download PDF

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RU2385347C2
RU2385347C2 RU2007100932/13A RU2007100932A RU2385347C2 RU 2385347 C2 RU2385347 C2 RU 2385347C2 RU 2007100932/13 A RU2007100932/13 A RU 2007100932/13A RU 2007100932 A RU2007100932 A RU 2007100932A RU 2385347 C2 RU2385347 C2 RU 2385347C2
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nucleic acid
polynucleotide sequence
lmp nucleic
lmp
isolated
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Хайко ХЭРТЕЛЬ (US)
Хайко ХЭРТЕЛЬ
Гарима БХАТТ (US)
Гарима Бхатт
Фолькер МИТТЕНДОРФ (US)
Фолькер Миттендорф
Кэрин Дж. ШАНК (US)
Кэрин Дж. ШАНК
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Басф Плант Сайенс Гмбх
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Abstract

FIELD: agriculture.
SUBSTANCE: nucleic acids and proteins are used in methods for production of transgenic plants and modulation of seed reserve substances levels. Preferably, reserve substances of seeds are lipids, fatty acids, starches or reserve proteins of seeds. Nucleic acids and proteins are also used in methods for modulation of seed size, seed number, weight, length of roots and size of plant leaves.
EFFECT: provides for released nucleic acids, which code proteins of lipid metabolism.
6 cl, 8 dwg, 3 tbl, 18 ex

Description

Текст описания приведен в факсимильном виде.

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Claims (6)

1. Выделенная нуклеиновая кислота белка липидного метаболизма (LMP), включающая полинуклеотидную последовательность, выбранную из группы, состоящей из:
а) полинуклеотидной последовательности, представленной в SEQ ID NO:16 или SEQ ID NO:17;
б) полинуклеотидной последовательности, кодирующей полипептид, представленный в SEQ ID NO:18;
в) полинуклеотидной последовательности, имеющей, по меньшей мере, 70%-ную идентичность последовательности с нуклеиновой кислотой LMP полной длины из упомянутых в а) или б);
г) полинуклеотидной последовательности, которая комплементарна нуклеиновой кислоте LMP полной длины из упомянутых в а) или б); и
д) полинуклеотидной последовательности, которая гибридизуется при строгих условиях с нуклеиновой кислотой LMP полной длины из упомянутых в а) или б),
причем кодируемый LMP белок функционирует как модулятор запасного вещества семян в растении.1. An isolated nucleic acid of a lipid metabolism protein (LMP), comprising a polynucleotide sequence selected from the group consisting of:
a) the polynucleotide sequence shown in SEQ ID NO: 16 or SEQ ID NO: 17;
b) a polynucleotide sequence encoding a polypeptide shown in SEQ ID NO: 18;
c) a polynucleotide sequence having at least 70% sequence identity with the full length LMP nucleic acid of those mentioned in a) or b);
d) a polynucleotide sequence that is complementary to the full length LMP nucleic acid of those mentioned in a) or b); and
d) a polynucleotide sequence that hybridizes under stringent conditions with a full length LMP nucleic acid from those mentioned in a) or b),
moreover, the encoded LMP protein functions as a modulator of the reserve material of seeds in the plant. 2. Выделенная нуклеиновая кислота LMP по п.1, где полинуклеотидная последовательность кодирует полипептидную последовательность SEQ ID NO:18.2. The selected LMP nucleic acid according to claim 1, where the polynucleotide sequence encodes a polypeptide sequence of SEQ ID NO: 18. 3. Выделенная нуклеиновая кислота LMP по п.1, где полинуклеотидная последовательность имеет, по меньшей мере, 90%-ную идентичность последовательности с нуклеиновой кислотой LMP полной длины из а) или б) по п.1.3. The isolated LMP nucleic acid according to claim 1, where the polynucleotide sequence has at least 90% sequence identity with the full length LMP nucleic acid from a) or b) according to claim 1. 4. Выделенная нуклеиновая кислота LMP по п.1, размещенная в экспрессирующем векторе.4. The isolated LMP nucleic acid according to claim 1, located in an expression vector. 5. Выделенная нуклеиновая кислота LMP по п.4, оперативно связанная с гетерологичным промотором, выбранным из группы, состоящей из семяспецифичного промотора, корнеспецифичного промотора и нетканеспецифичного промотора.5. The isolated LMP nucleic acid of claim 4, operably linked to a heterologous promoter selected from the group consisting of a seed-specific promoter, a root-specific promoter, and a non-tissue-specific promoter. 6. Выделенная нуклеиновая кислота LMP по п.4, оперативно связанная с ptxA промотором. 6. The isolated LMP nucleic acid of claim 4, operably linked to the ptxA promoter.
RU2007100932/13A 2004-06-16 2005-06-16 Molecules of nucleic acids, which code wrinkled 1-like polypeptides and methods of their application in plants RU2385347C2 (en)

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