RU2381803C2 - Method for prevention of nonspecific mastitis in lactating ewes and neonatal diseases of newborn lambs - Google Patents

Abstract

FIELD: veterinary.

SUBSTANCE: method consists in the fact that native tissue preparation "Uberalin", subcutaneously by 0.1-0.2 ml per 1 kg of body weight in three stages: 30 days before artificial insemination; 30 days before expected kidding; in the first 1-3 days after delivery.

EFFECT: method makes it possible to activate both humoral and cell factors of nonspecific resistance of animal organisms, provides for high level of main parametres of local protection of mammary gland tissues and improves their resistance to microbal factor.

6 tbl, 3 ex

Description

The invention relates to veterinary medicine, and more specifically to biological preparations having an adequate effect to stimulate and activate both general nonspecific resistance of the organism and local (local) resistance of the mammary gland tissues and genital organs in ewes to the microbial factor.

Mastitis is known to be an inflammation of the mammary gland, a widespread disease of lactating ewes in sheep breeding regions of the Russian Federation and foreign countries, while the incidence of animals with various forms of mastitis, according to several authors, ranges from 2.4 to 30.4% per year (B .Ya. Nikitin. Fighting sheep mastitis. - Stavropol, 1977; H.Worbes - Zur Mastitis bekamfung vei Mutterschafen // Tierzucht - 1981 - Yol.32. No. 2. p.541-543; H. Bostedt - Zur Mastidis beim Schat // Tierazztl, Uinsch - 1984-Jg, 39. No. 1 - s. 42-45; F. Filev - “Etiology of subclinical mastitis in sheep” // Ref. J. “Veterinary Medicine”, M., 1992, No. 2 , p.40-43; Tsedash Iev V. Ts. - “Problems of sheep husbandry in the Aginsky Buryat Autonomous Okrug” / Baikal Economic Forum - Collected reports and recommended books No. 6, II International conference Chita, 2003, p. 33-34).

It is noted that mastitis in ewes often occurs simultaneously (in parallel) with postpartum endometritis. If untimely detection (diagnosis) of mastitis and the provision of therapeutic and preventive care, from 10 to 12% of the animals die, and about 70% of the number of sick ewes are subjected to forced slaughter, which causes significant economic damage to sheep farms. In addition, it was found that the use of milk (colostrum) from patients with mastitis ewe with newborn lambs increases the incidence of the latter by respiratory diseases and gastrointestinal disorders 2-3 times more often and their mortality is 4-5 times more than lambs from healthy ewes.

It is also known that the most dangerous periods of the occurrence and development of mastitis in ewes are lactation and after beating the lambs from their mothers, and the frequency and degree of morbidity of animals is directly dependent on the state of nonspecific resistance of the body and local (local) resistance (resistance) of breast tissue to the microbial factor. This is convincingly evidenced by the fact that not all animal flocks (herds) fall ill with mastitis, but mainly only those in which a low level of nonspecific resistance of the organism and directly local (local) immunity of the mammary gland are recorded.

To activate and stimulate the nonspecific resistance of the body, many scientists recommend the use of biological preparations, among which the most effective are tissue preparations (Fibs, peat, aloe extract, suspension and placenta extract, peloiododistylate, agar-tissue preparation, L-103 hydrolysin, hemolysate, etc. ), which are prepared according to the method of Academician V.P. Filatov from animal and plant tissues (I.A. Kalashnik. Tissue preparations and their use. - M., 1976, 196 p.). These drugs have a wide range of effects on the body, contribute to the restoration of physiological processes, increase defenses. In addition, it is absolutely harmless and environmentally friendly drugs. However, these drugs are intended for medical purposes, and their industrial production for the needs of veterinary practice in the country is not imposed. The disadvantage of these drugs is their high cost, scarcity, and most importantly the complexity of their use in veterinary practice, which is associated with additional costs for the development of methods for their use for the prevention and treatment of sick animals.

The closest technical solution, selected as a prototype of this invention, is a tissue preparation - "Uteroline for the prevention and treatment of symptomatic infertility in cows", RF patent No. 2181832. Authors I.N. Zyubin, M.F. Zyubina, V.G. Chernykh, O.N. Samokrutova (publication November 27, 1999 BI No. 33). According to this prototype, a targeted tissue preparation is proposed - native uterolin, which contributes to the rapid restoration of disorders in humoral and cellular immunity, stimulates the mechanism of general nonspecific immunobiological reactivity of the body, as well as natural factors of local (local) immunity of the reproductive organs.

However, according to this prototype, which is practically devoid of drawbacks, it is impossible to successfully carry out highly effective prophylaxis and therapy of breast pathology, because, having selective specificity with respect to factors of local (local) immunity of the genital organs, uterolin native does not significantly affect the indices of the local breast protection.

The purpose of the present invention is to develop an effective method for the prevention of mastitis in lactating ewes and neonatal diseases of newborn lambs.

This goal is achieved by the phased use of a new tissue preparation of directed action - native uberaline (from the Latin word uder - udder) made of mammary gland tissue of animals that had nonspecific postpartum mastitis (RF patent No. 2201758 from 09/14/2000, authors I.N. Zyubin, M.F. Zyubina, V.G. Chernykh, O.N. Samokrutova, G.G. Nimatsyrenov). This drug contains in its composition all the essential amino acids, complexes of polypeptides, modified components of nucleic and carboxylic acids with high biological activity. It also revealed carbohydrates, macro- and microelements - calcium, phosphorus, manganese, zinc, copper, nickel, iron. The drug contains vitamins A, B ₁ , B ₂ , B ₁₂ . In addition, native Uberalin is characterized by the following properties necessary to achieve the objective of the invention:

- long-term (100%) biological stability during 2 years of storage;

- it contributes to the rapid restoration of disturbances in cellular and humoral immunity, stimulates the mechanism of general nonspecific natural resistance of the body, the level of hemoglobin, erythrocytes and leukocytes increases significantly in the blood of animals under the influence of uberalin, the level of bactericidal and lysozyme activity of serum increases, the phagocytic-absorbing and protective function of RES (see examples of specific use of the drug, table 1-2);

- It has a pronounced specific orientation of action on the mammary glands, while activating factors of local (local) protection of breast tissue, enhances their antimicrobial properties and resistance to the microbial factor, which prevents the development of the inflammatory process, and if there is a faster resolution.

Examples of specific effects and drug administration

Example 1. The effect of native Uberalin on the overall non-specific resistance of an ewes organism.

The studies were carried out on the basis of the Ononskoye industrial complex “Ononskoye” of the ZabNIISKh of the Russian Agricultural Academy.

As a model, 30 clinically healthy ewes of the Transbaikal fine-fleeced breed, aged 4-5 years old, were divided into three equal groups of 10 animals each.

To establish the background (initial) indicators before the administration of the drug, and then on the 5th and 10th days after the administration of the drugs, blood was taken from all ewes for examination for hemotological and immunological parameters.

The first (experimental group of animals was treated with a tissue preparation - native ureralin, the second (experimental) - with native uterolin. The preparations were administered subcutaneously at a dose of 0.1-0.2 ml per 1 kg of animal body weight. The dose was taken based on the dosage established for prototype.

Animals of the third group served control purposes, they were injected with physiological saline in the same dose as the test drugs.

The obtained materials are presented in Tables 1 and 2. From the data of Table 1 it can be seen that on the 10th day the ewes of the control group did not differ from those before the use of tissue preparations in the experimental groups.

In the 1st experimental group, where uberalin native was used after 10 days, the hemoglobin content increased by 10.5% compared to baseline and control. The number of red blood cells - by 11.55%, white blood cells - by 9.42%, lymphocytes by 11.93%.

The number of young, stab and segmented neutrophils and eosinophils decreased by 18.04%, 20.3% and 9.275%, respectively, at P <0.001, the number of basophils and monocytes did not change.

In the second experimental group, when using native uterolin, the hematological parameters for this period of time increased slightly: hemoglobin by 1.36%, which is 15.19% lower than that of ewes of the first experimental group, erythrocytes - by 2.62% or by 15.75% less than in the 1st experimental group and white blood cells - 0.65% or 10.76% lower than in ewes of the 1st experimental group. The number of lymphocytes remained unchanged.

The number of young, stab, segmented neutrophils and eosinophils decreased, respectively: by 4.48%; 0.4%; 0.18% and 0.5% in relation to the initial (background) data and control (at P <0.05).

Animals of the 1st experimental group treated with native uberalin had higher indices of natural resistance than the control and ewes of the 2nd experimental group (Table 2).

The total number of immunoglobulins in the serum of ewes was: in the 1st experimental group, 39.8 ± 0.7 g / l versus 24.4 ± 0.21 g / l in the 2nd group treated with uterolin. The lowest content of immunoglobulins was observed in the blood serum of the ewes of the control group - 22.7 ± 0.11 g / l.

After the use of native uberalin on the 11th day, in the ewes of the 1st group, the total protein content in the blood in relation to the control is higher by 23.7 g / L and 22.6 g / L than in the animals of the 2nd experimental group.

Table 1
The effect of native uberalin in comparison with the prototype (native uterolin on hematological blood parameters of ewes (n = 30) The studied indicators Background data, before drug administration After the use of drugs Control (estrofan) (M ± m) After 5 days (M ± m) After 10 days (M ± m) 2 3 four 5 6 Hemoglobin, × 10 g / l 65.0 ± 3.4 66.83 ± 3.2 75.7 ± 2.8 65.2 ± 2.3 66.80 ± 2.3 75.34 ± 3.3 Red blood cells, × 10 ¹² / l 5.77 ± 0.56 6.32 ± 0.42 7.34 ± 0.28 5.59 ± 0.26 6.29 ± 0.17 7.43 ± 0.26 White blood cells × 10 ⁹ / L 7.44 ± 0.58 7.68 ± 0.45 8.53 ± 0.71 7.39 ± 0.30 7.59 ± 0.38 8.49 ± 0.29 Neutrophils,%:
Young 1.17 ± 0.18 1.10 ± 0.29 1.28 ± 0.11 1.14 ± 0.12 1.12 ± 0.32 1.26 ± 0.14 Stab 6.30 ± 1.0 5.48 ± 0.40 4.52 ± 0.36 6.28 ± 0.56 5.20 ± 0.38 4.49 ± 0.33 Segmented 56.2 ± 0.78 52.0 ± 0.71 50.2 ± 0.31 56.0 ± 0.9 51.8 ± 0.96 49.8 ± 0.28 Eosinophils 2.2 ± 0.66 1.82 ± 0.23 1.80 ± 0.14 2.00 ± 0.8 1.82 ± 0.21 1.81 ± 0.14 Basophils 0.79 ± 0.25 0.83 ± 0.31 0.80 ± 0.40 0.80 ± 0.33 0.81 ± 0.27 0.80 ± 0.28 Monocytes 1.32 ± 0.41 1.32 ± 0.21 1.33 ± 0.62 1.32 ± 0.47 1.33 ± 0.19 1.29 ± 0.53 Lymphocytes 32.8 ± 2.17 33.7 ± 1.52 31.9 ± 1.21 32.5 ± 0.77 32.6 ± 1.18 32.5 ± 1.32 Note: in the numerator - indicators under the influence of native uterolin.

Table 2,
Immunological parameters of the blood serum of ewes after isolation of native uberalin and uteroloin (prototype) n = 30 (M ± m) The studied indicators Groups of animals Results of studies after the use of drugs in 5 days after 10 days Total protein, g / l Experienced - 1
Experienced - 2
The control 62.4 ± 0.22
62.8 ± 0.33
60.4 ± 0.28 62.2 ± 0.11 *
62.4 ± 0.8 **
60.2 ± 0.18 *** Albumin, g / l Experienced - 1
Experienced - 2
The control 32.8 ± 0.12
32.4 ± 0.33
31.4 ± 0.19 36.1 ± 0.16 *
36.0 ± 0.14 **
32.0 ± 0.10 *** Alpha globulin, g / l Experienced - 1
Experienced - 2
The control 21.0 ± 0.8
22.0 ± 0.3
30.1 ± 0.4 29.3 ± 0.7 *
28.8 ± 0.10 **
20.3 ± 0.12 *** Beta globulin, g / l Experienced - 1
Experienced - 2
The control 22.4 ± 0.7
22.6 ± 0.3
21.8 ± 0.9 30.7 ± 0.13 *
30.6 ± 0.14 **
21.8 ± 0.21 *** Gamma globulin, g / l Experienced - 1
Experienced - 2
The control 30.0 ± 0.9
29.8 ± 0.7
22.3 ± 0.6 39.8 ± 0.5 *
38.9 ± 0.22 **
22.7 ± 0.11 *** Bactericidal activity,% Experienced - 1
Experienced - 2
The control 25.0 ± 0.8
24.4 ± 0.10
20.1 ± 0.3 68.6 ± 0.23 *
44.7 ± 0.42 **
20.9 ± 0.6 *** Phagocytic activity,% Experienced - 1
Experienced - 2
The control 40.7 ± 0.27
42.1 ± 0.33
40.6 ± 0.27 63.8 ± 0.44 *
50.1 ± 0.22 **
34.3 ± 0.60 *** Note: * - the difference between the indicators of the 1st and control groups is significant (P <0.001).
** - the difference between the indicators of the 2nd and 1st experimental groups is significant (P <0.05).
*** - the difference between the indicators of the control and the 2nd experimental groups is significant (P <0.002).

The bactericidal activity of serum sludge on the 11th day increased to 68.6%, which is 47.7% higher than that of the ewes of the control group.

In the 2nd experimental group of animals where uteroline was used, this indicator was 23.9% less active than in animals of the 1st experimental group.

Uberalin native also contributed to a significant increase in cellular factors of the body's immune defense, which manifested itself in the activation of the phagocytic activity of neutrophils. So, in animals of the 1st experimental group after the use of the drug, the phagocytic activity of neutrophils was the highest - 63.8%, which is 29.5% more activity than the ewes of the control group (P <0.001). In ewes of the 2nd experimental group, phagocytic activity was 50.1%, which is 13.7% less than in the 1st experimental group of animals (P <0.05).

Thus, the results obtained indicate that the proposed tissue preparation, uberalin native, reliably activates both humoral and cellular factors of non-specific resistance of the animal organism.

The immunomodulatory and stimulating effects of native Uberalin on cellular and humoral defense mechanisms in the body reach a maximum 10 days after the use of the drug.

Example 2. The effect of native uberalin on factors of local protection of the mammary gland in lactating ewes.

It is known that the mammary gland (udder) of females has all the necessary non-specific and specific factors (mechanisms) of local (local) immunity. The main factors of local protection of the mammary gland include: poly- and mononuclear phagocytes, natural oxonins (lactoglobulins), as well as the immunoreactive enzyme - lysozyme - "M".

Unfortunately, while studying the etiopathogenesis of inflammatory processes of the mammary gland and developing therapeutic and prophylactic agents, very little attention is paid to the state of the factors of local protection of the mammary gland.

Observations show that the use of drugs for the prevention and treatment of animals with mastitis without taking into account their effect (effect) on the immunobiological activity of the mammary gland often does not have the desired effect. Many drugs suppress the tension of local protective reactions of the mammary gland, destroy mucopolysaccharides of the mucous membrane and often lead to serious consequences. Therefore, it is of great interest to take into account the state of factors of local (local) breast protection and their activation in the provision of therapeutic and prophylactic care to animals with mastitis.

Based on the foregoing, and in a set of measures to develop schemes and methods for the prevention of mastitis in lactating ewes with the use of the biological tissue preparation uberalin native, we conducted a study to study the effect of this drug on factors of local (local) resistance of the tissues of the mammary gland of ewes.

Due to the lack of background indicators of local mammary gland resistance in the ewes of the study zone, we studied these parameters in healthy and mastitis-affected ewes during various periods of lactation.

For these purposes, on the basis of the Ononskoye OPH breeding farm, two groups of ewes of analogues of the Transbaikal fine-fleece breed were formed, 10 animals in each group.

The 1st group included healthy animals, the 2nd - with the presence of subclinical and clinically pronounced forms of mastitis.

On the 2-3rd day after birth in the middle of lactation and before beating the lambs from the ewes from animals of both groups, the following material was taken: colostrum, milk, breast secret and subjected to laboratory research on the main indicators of local resistance of the breast - the number of somatic cells, the content lysozyme immunoreactive enzyme - M and the level of lactoglobulins.

During the experiment, the animals were regularly monitored with periodic medical examination, with particular attention to the condition of the mammary gland. The results of these studies are shown in table 3.

A comparative analysis of the materials in Table 3 shows that regardless of the period (period) of lactation in colostrum, milk and the mammary gland secret of ewes suffering from mastitis, the content of somatic cells is significantly higher in comparison with healthy animals. At the same time, the titer (activity) of the immunoreactive enzyme lysozyme M and the immune fraction of the γ-lactoglobulin protein, on the contrary, is reduced.

These data were used by us in further work as a criterion for assessing background indicators.

Table 3
Comparative indicators of local (local) breast resistance of healthy and sick mastitis ewes in different periods of lactation n = 20 The studied indicators Lactation Start the middle end healthy (M ± m) sick (M ± m) healthy (M ± m) sick (M ± m) healthy (M ± m) sick (M ± m) The number of somatic cells, thousand / ml ³ 203 ± 8.2 6504 ± 9.2 * 159.2 ± 8.16 3720 ± 5.13 * 340 ± 5.0 5988 ± 9.1 * Lysozyme activity - M, (ЗЗР in mm) 21.2 ± 0.6 16.6 ± 1.8 * 23.9 ± 0.3 17.3 ± 1.7 * 22.4 ± 0.6 16.2 ± 1.5 * Lactoglobulins,%
- α - alpha ............
- β - beta ............
- γ - gamma .......... 10.1 ± 1.2
10.3 ± 0.8
28.3 ± 2.0 14.3 ± 1.3 *
16.9 ± 0.2 *
20.8 ± 2.3 * 8.96 ± 1.6
9.82 ± 1.8
29.3 ± 2.0 10.5 ± 1.5 * 13.2 ± 0.6 * 22.6 ± 2.1 * 13.3 ± 1.4 13.5 ± 0.5 30.2 ± 3.7 17.7 ± 1.2 * 17.0 ± 1.7 * 27.6 ± 4.2 * *) P <0.05 in relation to healthy
ZZR - zone of growth inhibition of the test microbe on Petri dishes

A study of the influence of native uberalin on the main indicators of local immunity of the mammary gland was carried out in the above farm on two groups of lactating analogue ewes of 15 animals each. Before the formation of groups, all animals underwent a clinical examination and checked for the presence of subclinical (hidden) forms of mastitis. Animals with negative tests for subclinical mastitis were taken in the experiment.

The first experimental group of ewes was treated with native uberolin three times, subcutaneously at a dose of 0.1 ml per kg of body weight according to the following scheme - 30-20 days and the 10th day before birth. The second (control) group of ewes was not processed.

Before and after the administration of the drug, colostrum (milk) was taken from animals of both groups and examined for the main indicators of breast resistance. The research results are given in table.4.

From the data of Table 4 it can be seen that the main indicators of local protection of the mammary gland in the ewes of the experimental group after treatment with uberalin significantly differ from the indicators of the control group of animals.

So, the activity of the main criterion for the local protection of lysozyme - M in the ewes of the experimental group was 28.8 mm versus 16.9 mm in the control of non-specific resistance of this organ. In our opinion, this also explains the fact of a sharp increase in the number of somatic cells in the colostrum of the control group of animals.

During the final dispensary examination of animals at the end of the experiment, acute serous-catarrhal and 3 subclinical (hidden) mastitis were revealed in 3 ewes of the control group.

Thus, the developed scheme for the phased use of native uberalin provides a high level of basic indicators of local (local) protection of breast tissue and increases their resistance to the microbial factor, which allows us to recommend this scheme for the prevention of breast pathology in ewes.

Table 4
Comparative indicators of local protection of the mammary gland in ewes after treatment with a tissue preparation uberalin native (n = 30) The studied indicators Group of animals experienced control The activity of the immunoreactive enzyme lysozyme-M (ZZR in mm) 28.8 ± 0.6 16.9 ± 1.2 The number of somatic cells, thousand / ml ³ 207 ± 5.0 * 7320 ± 1236 Lactoglobulins,%
- α - alpha .............
- β - beta ..............
- γ - gamma ................ 16.86 ± 2.12
22.37 ± 3.14 *
26.63 ± 4.56 16.9 ± 5.1
20.4 ± 4.8
17.26 ± 4.09

*) P <0.05 with respect to the control;

**) P <0.002 with respect to the control.

The number of somatic cells, on the contrary, sharply increases in animals of the control group and reaches 7320 thousand / ml ³ versus 207 thousand / ml ³ in ewes of the experimental group, which indicates the inhibition of nonspecific resistance of breast tissue in animals of the control group. Significant changes also occur in the cytological picture of colostrum, so in ewes of the experimental group the number of lymphocytes from 38.8 ± 0.85 on the day of lambing by 5-7 days after decreased to 31.0 ± 0.02%, the number of eosinophils and monocytes, respectively , from 3.46 ± 0.2% to 0.33 ± 0.3% and from 25.0 ± 0.4% to 14.82 ± 0.88%.

In animals of the control group, over the same period of research, the number of eosinophils, neutrophils and lymphocytes is greater, and monocytes are less. Colostrum eosinophilic and neutrophilic leukocytosis in animals of the control group indicates a significant influx of RES cells from the blood into the mammary gland tissue, which is a protective factor for the suppression of nonspecific resistance of this organ. This also explains the fact of a sharp increase in the number of somatic cells in the colostrum of the control group of animals.

Example 3. Scientific and industrial experience on the use of native ureraline for the prevention of mastitis in ewes and neonatal diseases in newborn lambs.

We have revealed that mastitis in lactating ewes occurs simultaneously with postpartum endometritis in 13.62% of the total number of animals that have become stiff. Given this interconnection, a comprehensive scheme (methodology) for the use of biological preparations of uberalin and uteroline for the pharmacoprophylaxis of breast pathology and postpartum endometritis was developed and tested in a wide industrial experience.

The experiment was conducted on the basis of the Ononskoye collective farm. For experience in the flock of senior shepherd Mikhailov BD. 400 clinically healthy ewes were selected - analogues of the Transbaikal fine-fleeced breed at the age of 5-6 years. 30 days before their artificial insemination, the ewes were tagged and divided into two equal groups - experimental and control. Both groups were in the same flock and on the same diet.

According to the developed scheme (technique), tissue preparations were used by the ewes of the experimental group in the most critical periods of the physiological and immunological state of the body: before artificial insemination, in the last month of coagulation (pregnancy) and in the first 1-3 days after lambing (childbirth).

The drugs were prescribed to the ewes of the experimental group in the following sequence:

- 30 days prior to artificial insemination (to increase fertility), the native tissue preparation uberalin was administered subcutaneously at a dose of 0.1-0.2 ml per kg of animal body weight;

- 30 days before the proposed lambing - native Uberalin.

The drug was administered subcutaneously in the same dose. Repeated Uberalin native was also used in the first 1-3 days after lambing (childbirth).

The second group of ewes served control purposes and were not treated with drugs. During the experiment (10 months), the ewes of both groups and the offspring obtained from them were regularly monitored.

The results of a production experiment (experiment) showed that the administration of tissue preparations to ewes according to the above scheme ensured their fertility during the first sexual cycle and amounted to 98.6% in the experiment versus 72% in the control group.

Normal fruiting during childbirth in the experimental group was observed in 98% of animals. Prolonged pathological birth (weak contractions, attempts, premature discharge of amniotic fluid) in the ewes of the experimental group was observed at 32.4% less than in the control group of sheep. Retention of the placenta in the experimental group was only in 2 ewes, and in the control - in 27.

In the experimental group of ewes, there were 6.4 times fewer animals with postpartum subinvolution of the uterus, 6.8 times less with inflammatory processes in the genitals (postpartum endometritis). Acute postpartum mastitis in lactating ewes was diagnosed only in 7 experimental and 22 control ewes, or 31.4% more than in the experimental group.

Lambs born from experimental ewes were much faster and independently showed a sucking reflex in the first minutes after birth. The live weight of newborn lambs from experimental ewes exceeded that of lambs in the control group by 1.023 kg.

There were no stillborn lambs in the experimental group of ewes, and in the control group - 9. Weak lambs in the experimental group were only 2.9%, and in the control group - 39.45%, while the incidence of lambs by postnatal diseases (respiratory and gastrointestinal) and their death for the first time 10-20 days of life in the experimental group was observed 6.6 times less than in the control.

When examining blood serum for immunological parameters, the increase in live body weight and the survival rate of newborn lambs at the age of 20 days received from ewes from the experimental group compares favorably with the control group (Table 5).

Table 5
Immunological indicators, live weight gain and survival of newborn lambs at 20 days of age, obtained from experimental and control ewes n = 30 (M ± m) Group Weight gain (kg) Preservation (%) Bactericidal activity (%) Active lysozyme (%) Total protein (g / l) Immunoglobulin (mg / ml) Gamma Globulin (%) one 3.0 ± 0.4 98.3 58.2 ± 7.4 10.4 ± 0.9 70.2 ± 5 15.8 ± 2.4 24.1 ± 3.1 2 2.8 ± 0.5 73.1 46.3 ± 68 7.08 ± 1.1 69.4 ± 7 11.4 ± 1.9 17.9 ± 3.2

Similar indicators were obtained in the study of blood serum in lambs in the dynamics of growth and development at the age of one month and two months (Table 6).

Table 6
Indicators of natural resistance of young experimental and control groups at the age of one month n = 30 (M ± m) Group n Bactericidal activity (%) Lysozyme activity (%) Total protein (g / l) Immunoglobulin (mg / ml) Gamma Globulin (%) Experienced twenty 64.4 ± 4.5 9.4 ± 6.9 72.5 ± 5 16.9 ± 3.2 28.9 ± 4.9 Control twenty 64.9 ± 9.6 9.0 ± 2.1 73 ± 6 16.7 ± 4.1 29.3 ± 5.7

It should be noted that lambs born to uterus with high immune indices at 2 months of age significantly exceed their peers obtained from low-resistance uterus in bactericidal activity by 28%, lysozyme by 24%, immunoglobulins by 18% and gamma globulins by 21 % There were 116 lambs per 100 sheep in the experimental group of ewes, and in the control group, 91, i.e. this pattern of drug use allows you to get 25 lambs per 100 ewes more, and the live weight of lambs from experienced ewes to beating was 4 kg higher than in the control group.

Thus, the scientific and industrial experience and the results obtained give us reason to conclude that it is feasible and promising to use native tissue preparations uteroline and uberalin for the purpose of comprehensive pharmacoprophylaxis of postpartum mastitis and endometritis in lactating ewes, as well as the prevention of postnatal diseases of newborn lambs.

Our studies have shown that native tissue preparations significantly contribute to the activation of the immunological activity of the body and increase local factors of nonspecific resistance of the reproductive organs (uterus and mammary gland) in lactating ewes. They do not have a side (negative) effect on the body of pregnant animals and intrauterine development of the fetus. The drugs do not have an allergic, embryotoxic, anaphylactogenic and histamine-like effect. Regular use of uteroline and uberalin according to the scheme developed by us during 2001-2005. the farm allowed to get 130-151 lambs from 100 ewes for beating.

Claims (1)

A method for the prevention of nonspecific mastitis in lactating ewes and neonatal diseases of newborn lambs, including the introduction of a native tissue preparation of uberalin, characterized in that uberaline is administered to the ewes subcutaneously at 0.1-0.2 ml per 1 kg of body weight in three stages:
30 days before artificial insemination;
30 days before the alleged lambing;
in the first 1-3 days after birth.