RU2371216C2 - Method of focused local therapeutic and/or cosmetological effect on human tissues and device for method implementation - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: group of inventions concerns medicine and cosmetology and can be applied in focused local therapeutic and/or cosmetological effect on human tissues. It involves uniform activation of photodynamic therapy device without contact to damaged tissue by light exposure directly before medicine application. Then the medicine is applied onto damaged tissue, medicine excess removed. Photodynamic therapy device is activated by device including container with storage tank for photodynamic therapy device. One of the tank walls features optic system communicating with light source. Container has a lid. When the lid is removed, activation device included in electric unit closes electric circuit. Electric unit is connected to light source and also activates power unit and deactivation device. Deactivation device can be made in the form of time relay or microprocessor.

EFFECT: prevented complications related to body irradiation by activation of photodynamic therapy device and by body contact to inactivated photodynamic therapy device capable or preserving activated state long enough to affect damaged tissues.

7 cl, 3 dwg, 2 ex

Description

The present invention relates to methods for influencing the tissues of the human body due to the photodynamic transformation of the compositions (agents) into a biologically active state and can be used to treat diseases of the skin and its appendages (nails, hair) and mucous membranes, as well as to achieve cosmetic effects on the skin and its appendages (nails, hair). Exposure to tissues can cause both therapeutic and cosmetic effects. Photodynamic transformation of the compositions (means) provided for by the method is carried out using a special device that constructively combines a container for storing the composition (means), a light source and a special electrical module that controls the operation of the light source. The method is intended for patients in need of medical (dermatological) help, as well as for people in need of cosmetic procedures.

Despite the rapid development of science and technology, the problems of treating diseases that affect the surface tissues of a person remain relevant. Until now, diseases such as psoriasis, neurodermatitis, and some others remain almost incurable. In the treatment of such diseases, only a temporary effect is achieved and sooner or later relapses occur. Moreover, due to the deterioration of the environmental situation, especially in urban conditions, the influence of adverse factors (pollution of the atmosphere and water environment, intense electromagnetic radiation from mobile phones and computers, an overdose of the ultraviolet component of solar radiation due to a decrease in the ozone layer, etc., is increasing). ) on the skin and mucous membranes of a person. This leads to an increase in the number of chronic skin diseases, an increase in the incidence of cancer and skin melanoma, the appearance of cosmetic problems - premature aging of the skin, alopecia, pigmentation disorders.

Based on these facts, the problem of finding new therapeutic methods of exposure in pathologies and anomalies of the surface tissues of the human body remains very relevant.

Among modern medical methods in dermatology and cosmetology, methods based on photodynamic effects — phototherapy, photodynamic therapy — are promising. In a number of cases, these methods make it possible to achieve the remission of certain diseases and even complete clinical recovery, and their use in cosmetology leads to positive cosmetological effects. The scientific, technical and patent literature describes many methods of phototherapy (FT) and photodynamic therapy (PDT) used for therapeutic and cosmetic purposes. All of them can be divided into two groups.

The first group includes phototherapy methods based on the fact that irradiation of the human body is carried out with the light of various wavelengths that directly affect target molecules in the human body; these methods do not imply a preliminary artificial introduction of substances containing the above target molecules into the human body. In this case, depending on what characteristics of light are used and on which target light quanta act, target molecules can transform into a new state that directly or indirectly affects the vital processes of tissues. This can lead to: 1) destruction of pathologically altered tissues and elimination of their pathological elements from the body and / or 2) normalization of physiological processes in tissues. Examples of the above methods are methods described in patent documents (Dees N.S., Wachter E.A. Treatment of pigmented tissues using optical energy. US Patent No. 7036516 dated 05/02/2006; Perricone NV Apparatus for skin treatment. US Patent No. 7066941 dated 06/27/2006; Xie P. Method and apparatus for epidermal treatment with computer controlled moving focused infrared light. US patent No. 6149644 from 11/21/2000) and the methods described in the scientific literature (But WS, Ying SY, Chan PC, Chan HH Treatment of port wine stains with intense pulsed light: a prospective study. Dermatol Surg. 2004 Jun; 30 (6): 887-890). Despite the fact that these methods are relatively easy to reproduce, since they require only a light source and, in some cases, devices for conducting light radiation to a pathological site, they have limitations in their application. The main disadvantages of these methods are the lack of effectiveness in the treatment of chronic diseases, as well as the conditions existing in a number of patients in which phototherapy is contraindicated - for diseases of the skin and skin appendages associated with exposure to light radiation (photodermatitis, light (solar) urticaria, polymorphic light rash photokeratitis (actinic keratitis), photochemical granuloma, photochemical reticuloid).

The second group includes methods based on other principles of phototherapeutic effects on pathological, abnormal and normal tissues of the body. All these methods of so-called photodynamic therapy (Babilas R., Karrer S., Sidoroff A. et al. Photodynamic therapy in dermatology. Photodermatol Photoimmunol Photomed 2005 Jun; 21 (3): 142-149; Moan J., Peng Q. An outline of the hundred-year history of PDT. Anticancer Res. 2003 Sep-Oct; 23 (5A): 3591-3600) are based on a combination of several stages of the treatment procedure: 1) the introduction into the body in one way or another of special substances - photoactive agents, it is also possible to remove excess introduced agents; photoactive agents by themselves do not have a direct therapeutic effect on pathologically altered tissues; 2) the exposure during which the introduced photoactive agents are distributed in the body - they can be dispersed throughout the body or selectively concentrated in a particular tissue; 3) irradiation of pathological tissues with light, during which a photoactive agent located in the tissues of the body, under the influence of photon energy, turns into a photoactivated substance, which has the necessary healing properties; the transformation process is called photodynamic activation or photodynamic transformation. Examples of such methods are those described in the patent and scientific literature, for example Babilas P., Karrer S., Sidoroff A. et al. Photodynamic therapy in dermatology. Photodermatol Photoimmunol Photomed. 2005 Jun; 21 (3): 142-149. These methods differ from each other: 1) the content of the three above-mentioned stages of PDT; 2) on the use of various compositions of photoactive agents; 3) according to the therapeutic effect, which determines the use of each specific method.

Based on the above patent and scientific and technical documents, it can be argued that the implementation of known methods includes the following options. The first stage of photodynamic therapy can be based on the use of photoactive agents with different chemical structures. Photoactive agents can be introduced / applied: 1) topically on the skin, mucous membranes, in the wound channel, places of ulceration, in the tissue of the surgical field; 2) systemically parenteral, enteral, through enemas; 3) locally - through catheters, in an endoscopic manner. The second stage - exposure - for different authors can vary in time from 0 seconds (when the administration of photoactive agents is carried out simultaneously with the third phase of PDT - irradiation) to the time intervals measured in hours, which is necessary to selectively achieve the maximum concentration of the photoactive agent in pathologically altered tissue. The third stage - light irradiation - depending on the characteristics of the photoactive agent used and the expected photodynamic effects, can be constant, intermittent or pulsed, monochromatic or non-monochromatic, coherent or incoherent, single, double or multiphoton, can have different intensities and spectral characteristics in the range from ultraviolet to infrared light, including the visible region.

The main positive aspects of the described methods are: 1) the high efficiency of the action of the photoactivated agent on the tissue; 2) the selectivity of the action of the photoactivated agent, which is expressed in the fact that the therapeutic effect of the photoactivated agent is aimed only at pathologically altered tissues. The authors of these methods sought to minimize the effect of the photoactivated agent on healthy tissues. But these methods have significant disadvantages. The main one is the same drawback that was previously described in the characteristics of phototherapy - the conditions existing in a number of patients in which phototherapy is contraindicated - for skin diseases and skin appendages associated with exposure to light radiation (photodermatitis, light (solar) urticaria, polymorphic light rash, photokeratitis (actinic keratitis), photochemical granuloma, photochemical reticuloid). Thus, the mentioned methods of photodynamic therapy cannot be applied in some groups of patients. Closest to the proposed solution and the selected author as a prototype is the "Improved method of targeted local treatment of the disease" (publication No. 2001103133 from 2002.11.27; WO 00/07515). The known method allows therapeutic photodynamic effects on the affected tissue and involves the following implementation. The method includes the steps of applying a PDT agent to said affected tissue to form a treatment zone; removing an excess amount of the agent, and exposing the indicated treatment zone to light to activate the agent associated with the diseased tissue, in which said light enters the specified treatment zone. The authors also claim in this method the application of various characteristics of light radiation, various means of conducting light to the irradiated area. The method may include the use of a device that transfers heat supplied to the treatment area to increase activation of PDT agents. As photoactive agents for PDT in the prototype, it is supposed to use both the standardly used means for PDT and the means for PDT having an aiming guided part. The prototype involves various methods of introducing a PDT agent into tissues, including topical application, injection, capillary, catheter, parenteral administration. The undoubted advantages of the method described in the prototype are: 1) the effectiveness of the therapeutic effects of photoactivated substances; 2) the selectivity of the claimed method of photodynamic therapy, based on the selectivity of the delivery of drugs for PDT in the affected tissue; 3) the method may include a diagnostic stage (introduction of an indicator), which improves the quality of both diagnosis (both in terms of making a general diagnosis, and in terms of localization and boundaries of pathologically altered tissue), and therefore the effectiveness of the treatment of the disease. However, the known method has significant disadvantages.

Firstly, in the prototype, the photoactive agent is exposed to light exposure, being in tissues that require therapeutic exposure. In this case, simultaneously with the photoactive agent, all structural elements of body tissues are exposed to irradiation - not only pathological, but also normal. Light radiation can have not only a beneficial effect on the human body. There are a number of pathological and abnormal conditions called photopathies, in which the effect of light radiation on tissues leads to a disruption of normal physiological processes in tissues. These conditions — photopathies — include a number of skin diseases defined by the international classification of diseases as “Diseases of the skin and skin appendages associated with exposure to radiation” (L55-L59: Diseases of the skin and skin appendages associated with exposure to radiation: L56.2. Photodermatitis (photocontact dermatitis) L56.3. Light (solar) urticaria. L56.4. Polymorphic light rash. L57.0. Actinic (or photochemical) keratosis. L57.1. Actinic (or photochemical) reticuloid. L57.5. Actinic (or photochemical) granuloma), as well as some porphyrinopathies, vitiligo. Light allergy is described as an independent nosological unit (Ichihashi M. UV-induced skin damage and photo-allergic disease. Arerugi. 2007 Jul; 56 (7): 670-678). In addition, in a number of works it is proved that light in the ultraviolet range, which is often used in PDT, has a carcinogenic effect, leading to skin cancer. In particular, the mutagenic effect of intense light is due to the fact that the photon energy may be enough to move the electron in the atoms that make up the structure of human tissue molecules from the internal to the external level, transfer the atoms to an unstable state and increase their tendency to participate in chemical reactions, including mutations affecting the genetic apparatus of cells. Frequent PDT complications resulting from exposure to intense light on the skin are inflammation and the transdermal fluid loss syndrome known as TEWL (Faurschou A., Wiegell SR, Wulf N.C. Transsepidermal water loss after photodynamic therapy, UVB radiation and topical corticosteroid is independent of inflammation. Skin Res Technol. 2007 May; 13 (2): 202-206). Other adverse effects from exposure to intense light can be immune suppression (Ullrich S.E., Alcalay J., Applegate L.A., Kripke M.L. Immunosuppression in phototherapy Ciba Found Symp. 1989; 146: 131-9; discussion 139-147). In addition, intense light can have adverse cosmetic effects, in particular intense light leads to photoaging of the skin and plays a role in such processes as premature aging (withering) of the skin. The above photopathies are not rare exotic diseases, they are quite widespread among the population of developed countries (Bryden AM, Moseley N., Ibbotson SH et al. Photopatch testing of 1155 patients: results of the UK multicentre photopatch study group. Br J Dermatol. 2006 Oct; 155 (4): 737-747.). These conditions may be contraindications for the implementation of the method described in the prototype. Secondly, in the prototype, therapeutic or indicator and therapeutic agents for photodynamic exposure in an inactive state are introduced into the body in one way or another to affect tissues that require therapeutic exposure. In medical practice, there are cases (they are also described in the medical literature) in which a photoactive agent, being in an inactive state, is an allergen to humans (Harries MJ, Street G., Gilmour E., Rhodes LE, Beck MH Allergic contact dermatitis to methyl aminolevulinate (Metvix) cream used in photodynamic therapy. Photodermatol Photoimmunol Photomed. 2007 Feb; 23 (1): 35-36.), while its photoactivated form may not be an allergen; such cases are a contraindication to the application of the method described in the prototype.

Thirdly, the application of the known method does not take into account the presence in the tissues of components that inhibit the process of transformation of a photoactive substance into a photoactivated substance with a therapeutic effect at the time of exposure to light. For example, powerful pigmented and / or antioxidant systems existing in the tissues can inhibit the photoactivation of certain medications. This leads to both a decrease in the efficiency of the method and a fundamental impossibility to use certain substances as photoactive.

Fourthly, the method of tissue illumination proposed in the prototype assumes an uneven microdistribution of the photoactivated agent in the tissues due to the uneven photoactivation effect of light, since the light is distributed non-linearly in the tissues (Bass L.P., Nikolaeva O.V., Kuznetsov BC and other. Modeling the propagation of optical radiation in the phantom of biological tissue on the superecomputer MVS1000 / M; 2005). It also reduces the therapeutic effectiveness of the method.

All these shortcomings not only quantitatively reduce the effectiveness of the method, but also qualitatively limit its scope, not allowing it to be used to treat large groups of people.

The objective of the invention is the creation of a new effective method of therapeutic and / or cosmetological effects on the tissues of the human body, which allows the use of photodynamic methods in wider groups of patients, including those suffering from photopathies, and eliminating the occurrence of side effects.

The problem is solved by the proposed method, in which the composition (means) for photodynamic effects (photoactive preparation) is subjected to light irradiation immediately before application to the tissue using a special device. In the process of this, the composition is activated, transforming from photoactive to photoactivated form, acquiring new useful biologically active properties. Then the photoactivated composition is applied to the target tissue, where it has a therapeutic and / or cosmetic effect. A photoactive composition (photoactive agent) can have various liquid and soft forms: true, colloidal and micellar solutions, gels, creams, mono- and polydisperse systems, emulsions, vesicular and liposome systems, etc. In the proposed method, the tissues are exposed to already photoactivated product, which is in a biologically active state. The method involves the local use of a photoactivated product on the skin and its appendages (hair, nails), as well as on mucous membranes. In the proposed method, human tissues are not exposed to radiation. To implement this method, a special device is used, which is designed in such a way that it is both a packaging container for storing a photoactive composition and a device that brings this composition into a photoactivated state by generating light radiation. For this, the light source is structurally connected directly with the container for storing the photoactive composition and its processing with light. The device includes a special electric control unit for the operation of the light source. The device that generates light radiation for photoactivation of agents is designed in such a way as to ensure, first of all, the uniform effect of light radiation on a photoactive product in a limited capacity, immediately before its application to the surface of target tissues. As light sources, the method involves the use of incoherent and coherent, monochromatic and non-monochromatic, constant and pulsed light sources of the ultraviolet region (from 280 nm to the border of the visible region of the spectrum), the visible region of the spectrum and infrared region of the spectrum (from 840 nm to 1100 nm and from 7000 nm to 14000 nm). The choice of the light source and the type of light exposure depends on the type of photoactive composition used. The photoactive composition at the time of photoactivation is located in a special container outside the tissues of the human body; this avoids the influence of tissue components that inhibit the photoactivation process. Due to this, both the efficiency of the method increases, and a fundamentally new opportunity arises to use substances as a photoactive composition for which photoactivation in tissues is impossible. The targets for the photoactivating effect of light in a photoactive composition can be not only photoactive agents that transform into substances with a therapeutic or cosmetic effect, but also auxiliary components of the composition, for example, substances and / or structures that ensure the delivery of the composition (agent) deep into the tissues through the penetration of the composition through the surface border of the tissue (for example, transdermal transport). The method involves two options for transforming the photoactive composition in the container into an activated form: single and multiple. In the first case, the device is disposable due to the irreversibility of the conversion of the photoactive composition into a photoactivated substance and is disposed of after use. In the second case, the device and the formulation of the photoactive composition allow repeated use, including: 1) the photoactivation phase - the transformation of the inactive composition into a form capable of exerting the desired pharmacological / cosmetological effect; 2) the stabilization phase - the existence of the composition in activated form for a time sufficient to provide the desired effect on the tissue (including delivery and the direct pharmacological / cosmetic effect); 3) deactivation phase - the return of the activated unused composition remaining in the container to its original inactive form, capable of repeated photoactivation; 4) repeated repetition of the above procedures through re-activation (reactivation) and deactivation. When used repeatedly in photoactive compositions, formulations are used that allow the composition to maintain its photodynamic potential for a sufficiently long period of time, withstanding multiple transformations between two states of the composition - photoactive and photoactivated.

The proposed method is as follows.

A photoactive composition (preparation) containing components used for photodynamic effects on tissues is stored in the container of a special device for implementing the proposed method. The container lid opens immediately before use. Opening the lid automatically turns on the light source located in the immediate vicinity of one of the walls of the container or in the immediate vicinity of the bottom of the container. The light emitted by the light source penetrates into the interior of the container through a special optical system that provides uniform light treatment of the photoactive composition (means) located in the container. The physical characteristics of the light source and the light radiation itself depend specifically on the type of PDT agent included in the photoactive composition. During the exposure, which can last from 5 seconds to several minutes also depending on the type of PDT product, the photoactive drug is converted into a photoactivated substance, which acquires useful biologically active (therapeutic, cosmetic) properties. This process is called photodynamic activation. The processing conditions for a particular photoactive agent (or agents) included in the composition (including the choice of a light source by wavelength, power, exposure duration, etc.) are selected based on information accumulated by modern photochemical science (Gelfond M.L., Zaretsky A.M., Kaplan M.A. Guidelines for the use of photodynamic therapy. - Moscow, 2005; Tardivo, JP et al. Methylene blue in photodynamic therapy: From basic mechanisms to clinical applications. Photodiagnosis and Photodynamic Therapy, Sep 2005 No. 2 (3), p. 175-191; Awan MA., Tarin SA Review of photodynamic therapy. The surgeon: journal of the Royal Colleges of Surgeons of Ed inburgh and Ireland, Aug 2006, No. 4 (4), p. 231-236.). In addition, the choice of exposure time and characteristics of light radiation is carried out taking into account the norms regulated by pharmacopeia articles corresponding to drugs for FTD. Immediately after exposure, the amount necessary for the procedure (usually from 5 to 15 ml of the preparation is consumed per 100 cm of the surface) of the photoactivated preparation with a tampon, or spatula, or other material (sponge, tissue, bandage, etc.) is transferred from the container to the problematic surface of the human body or on its mucous membranes. The transferred material is evenly distributed over the problem area. If necessary, after 2-5 minutes, the excess of the applied photoactivated composition (agent) is removed. The multiplicity and duration of the course of the above procedures depend on the type and severity of the pathological process. The first procedure is carried out under the supervision and with the direct participation of the attending physician with the provision of detailed instructions regarding its implementation. The patient can carry out the following procedures independently in conditions comfortable for him.

Figure 1 shows a schematic diagram of a device for implementing the proposed method, depicting a device with a closed cover (7). The device comprises a container (6) for storing means for PDT. An optical system (4) is built into one of the walls (5) that limit the container's capacity, which ensures the transmission of light from the light source (3) into the cavity of the container for storing the photoactive composition and uniform irradiation of the entire internal space of the container. The light source (3), coupled by means of housing elements with the aforementioned optical system (4), is controlled by a special electrical module (2). This module, the circuit diagram of which is shown in figure 2, incorporates a power supply (8); a switching device (9) of the electrical module, consisting of a switch that mechanically closes the electrical circuit when the cover is removed, or a sensor that responds to changes in the electromagnetic characteristics of the device when the cover is removed; a shutdown device (10) of the electrical module, consisting of a time relay or a microprocessor programmed to turn off the power from the light source after a certain time; a device (11) that provides the current source with the required characteristics (voltage, current, and frequency characteristics) to the light source (3). An electrical module, as shown in FIG. 2, is connected to the light source (3) by a single electrical circuit. The electric circuits, indicated in FIG. 2 by a dashed line, connect all of the above components of the electrical module and the power source into a single electrical circuit. All of the above parts of the device for implementing the proposed method (see figure 1), with the exception of the cover (7) are structurally combined in a single housing (1); the cover (7) is mechanically attached to the housing (1).

A device for implementing the proposed method works as follows. Opening and removing the lid (7) of the container with the photoactive composition leads to automatic activation of the electrical module (2), which provides power to the light source (3) and turns it on. The automatic switching on of the electrical module can occur due to the fact that the removal of the cover leads to mechanical switching (circuit closure) and the supply of electrical power to the electrical circuits of the specified module, or due to the fact that the removal of the cover is accompanied by a change in electromagnetic properties (capacitance, inductance ) of the device, this is fixed by a special sensor, which is part of the electrical module. After a certain period of time, which depends on the type of photodynamic means used (see above), the electrical module (2) turns off the light source (3). Turning off the electrical module and turning off the light source is provided due to the operation of the time relay or due to the action of the microprocessor; one of these devices is also part of the electrical module (2). To re-enable, you must first completely close the lid (7) of the container, and then open it again; the procedure for turning on the light source is completely repeated.

The advantages and advantages of the proposed method is the following.

1) The absence of light exposure to human tissue, and therefore pathological conditions that occur in response to the effect of light radiation on the human body (photopathy) are not contraindications for the application of our method.

2) Ensuring a uniform distribution of photoactivated substances in the PDT due to the uniform exposure to light during photoactivation due to the presence in the design of the device for implementing the proposed method of a special optical element.

3) The possibility of using the method for more efficient delivery of a therapeutic or cosmetological photoactivated composition across the surface border of a tissue (for example, transdermal transport) due to the effect of light on the photoactive components of the excipients of the base composition.

4) The possibility of using fundamentally new substances as photoactive agents for which photoactivation inside the tissues is impossible, as well as using substances as active substances with a therapeutic / cosmetic effect, the active forms of which exist for relatively short periods of time - within minutes and hours.

5) The ergonomics of the method, expressed in ease of use: the patient can easily carry out the procedure on their own at home, in comfortable conditions. During periods of exacerbation of certain dermatological diseases, the appearance among strangers for some patients may be a difficult psychological test leading to stress, which does not contribute to a quick cure. Despite the large number of structural elements, the device for implementing the proposed method is compact, which is undoubtedly convenient for its use.

The proposed method was used in the treatment of 15 people (men and women) suffering from various forms of psoriasis and atonic dermatitis; in addition, the method was used in cosmetology practice in 21 people.

The use of the proposed method can be illustrated by the following examples, which are not limiting.

Example 1. Patient T., male, 41 years old. Diagnosis: psoriasis, skin form, in the acute phase. A history of intolerance to PUVA therapy (one of the methods of photodynamic therapy), expressed in the development of a pronounced inflammatory reaction of the skin in the area exposed to ultraviolet radiation during classical photodynamic therapy sessions. Clinically, there are multiple polymorphic foci of a specific psoriasis skin lesion - acanthosis, located on the scalp, itching of the scalp. The sizes of the foci from 1 to 9 cm ² . In connection with the history, the use of traditional photodynamic therapy is contraindicated. For the implementation of treatment as a method of therapeutic effect, the method described in the present invention was applied. A 0.5% radachlorin gel was used as the photoactive agent. The device for implementing the proposed method was equipped with an ultraviolet light source with a predominant radiation spectrum in the range of 220-400 nm. The agent (composition) was irradiated for 5 min — a time sufficient to activate radachlorin. The patient kept the lid of the container open for 5 minutes, after which approximately 7-15 ml of the irradiated preparation was transferred to psoriasis-affected areas of the scalp and was evenly distributed over their surface with a tampon. After 10 minutes, excess applied product was removed with a tissue. After 5 such sessions, repeated after 48 hours, which the patient spent on his own at home, there was a significant improvement, expressed in remission of skin manifestations of psoriasis. The development of inflammation of the scalp during treatment was not observed.

Example 2. Patient K., a woman, 39 years old. I applied for cosmetic help with complaints of wrinkles in the forehead. A history of photodermatitis. A visual examination of the frontal region, as well as an instrumental examination performed using a ProScop HR skin microscope (Skin & Hair Handheld Video Microscopes, USA), confirmed the presence of pronounced wrinkles. Figure 3 shows a micrograph of wrinkles before cosmetic treatment (A). The proposed method was applied as a method of cosmetological effect. As a photoactive agent, a composition was used, which is a complex emulsion of cyclomethicone / dimethicone in water, known in cosmetology, containing 0.5% metal minolevulinate The device for implementing the proposed method was equipped with a light source with a radiated wavelength in the region of 320-500 nm. The container with the contents was kept with the lid open for 5 minutes - time sufficient for photoactivation of the composition. Then about 3-5 ml of the irradiated preparation was transferred on the skin of the frontal area and evenly distributed on its surface using a cosmetic swab.After 15 minutes, the excess applied product was removed with a cosmetic towel. The first procedure was performed by a doctor with a detailed explanation of the features of the execution, the subsequent procedures were carried out at home at a convenient time. After 30 such sessions, repeated daily, the patient began to significantly improve, expressed in smoothing out wrinkles, as well as reducing their depth. Figure 3 presents the micrograph (B), objectively confirming these results. The proposed method allows to achieve: 1) an effective therapeutic result in patients who do not tolerate the methods of traditional phototherapy due to their associated diseases - photopathies; 2) an effective cosmetological result in people who cannot tolerate the methods of traditional photocosmetology due to their associated diseases - photopathies. It should be noted the positive attitude of patients associated with the ability to be treated at home without the use of special, sometimes bulky, irradiating devices.

The above examples prove that the proposed method can be successfully used in the practice of a dermatologist, and also gives a pronounced cosmetological effect when applied in cosmetology practice.

Claims (7)

1. A method of directed local therapeutic and / or cosmetic effects on human tissue, comprising applying a means for photodynamic therapy to the affected tissue, followed by removal of an excess amount of the agent, characterized in that immediately before applying the agent, it is uniformly activated in the absence of contact with the affected tissue by light exposure. 2. The method according to claim 1, characterized in that incoherent or coherent, monochromatic or non-monochromatic, constant or pulsed light source of the ultraviolet region — from 280 nm to the boundary of the visible region of the spectrum — or the visible region of the spectrum, or infrared, is used as light sources; spectral ranges from 840 to 1100 nm and from 7000 to 14000 nm. 3. The method according to claim 2, characterized in that the choice of light source depends on the type of means used for photodynamic therapy. 4. The method according to claim 1, characterized in that the time and variant of the light exposure for photodynamic therapy is determined depending on the type of means used for photodynamic therapy. 5. The method according to claim 1, characterized in that the light exposure can be single and multiple. 6. Device for activating means for photodynamic therapy, containing a container with a container for storing means for photodynamic therapy, one of the walls of which has an optical system connected to a light source, characterized in that the container is equipped with a lid, which is capable of removing the electric circuit circuit by a switching device as part of an electrical module connected to a light source and also including a power supply and a switching device. 7. The device according to claim 6, characterized in that the shutdown device is made in the form of a time relay or microprocessor.

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