RU2361417C2 - Feed with glucozamin and willow extract for prevention of arthrosis in animals - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: invention is related to veterinary and production pet food. Feed contains glucozamin salt, dry willow extract, vitamin complex "Bitam", versene, sodium metabisulfite, purified water, food ingredients at the following ratio of components, wt %: glucozamin salt 2.50, dry willow extract 0.15, vitamin complex "Bitam" 1.20, versene 0.20, sodium metabisulfite 0.30, purified water 5.00, food ingredients up to 100.00.

EFFECT: production of preparation with anti-arthrosis action, reducing clinical aspects of osteoarthrosis in dogs.

2 tbl, 4 ex

Description

The invention relates to veterinary medicine and for the development of feed with a specific composition and ratio of components for internal use in domestic animals (in particular dogs) with osteoarthritis.

In Russia, the following drugs are known that are used to treat osteoarthrosis in veterinary medicine: rumalon, hyalovet, potatoes [Abramova MA Pharmacological guide of the veterinarian. / Abramova M.A. - Rostov-on-Don: Phoenix, 2003 - 137 p.]. Closest to the proposed invention is patent RU No. 2130310 ("Composition is a dosage form of anti-arthritic glucosamine hydrochloride for internal use"). This composition is taken as a prototype.

The disadvantages of this composition include the difficulty in ensuring voluntary administration of the drug by domestic animals, the long term of use to achieve a therapeutic effect, the lack of components that increase the anti-inflammatory activity of glucosamine (arthrosis is usually accompanied by inflammation). Glucosamine salts have a pronounced salt-acid unpleasant taste (FSP 42-0314-1478-01 - Glucosamine). This circumstance, when prescribing the drug inside the pet, leads to the fact that the animals refuse to voluntarily take it. In such cases, two ways of giving drugs are known. The first way is to mix the drug with the usual food of the animal. In this case, there is no guarantee of the full intake of the drug. The second way is to give the animal a drug by force, using tweezers or forceps [Sozinov V.A., Ermolina S.A. Modern treatments for cats and dogs. - M., 2004. - P.5]. In this case, a 100% intake of the drug is guaranteed, but stress in the animal is inevitable. In this regard, the use of this composition for internal use in domestic animals is difficult.

In addition, it is known that tablets for internal use are recommended to be drunk with a large amount of water, otherwise highly concentrated solutions form at the place of their dissolution in the gastrointestinal tract. Solutions of tablets of glucosamine salts have a low pH level (FSP 42-0314-1478-01 - Glucosamine) and can irritate the gastrointestinal mucosa. This fact also complicates the use of tablets in animals.

Therefore, expanding the arsenal of feed compositions, characterized, on the one hand, by anti-arthritic, anti-inflammatory effects, and on the other, by pleasant organoleptic and convenient consumer properties, is an urgent task.

The objective of the invention is to develop a method for producing food based on glucosamine salt and dry willow extract, characterized by anti-arthritic, anti-inflammatory effects and the absence of an unpleasant taste.

The technical result from the use of the invention is achieved in that in order to obtain the target product, the following ingredients are introduced into the feed composition: glucosamine salt, dry willow extract, Vitam vitamin complex (TU 9355-004-34058560-01 - 100 g of the vitamin complex contain vitamins: A - 300,000 ME, D ₃ - 30,000 IU, E - 5 g, B ₁ - 1.1 g, B ₂ - 0.3 g, B ₆ - 0.3 g, PP - 0.5 g, K - 0.315 g , B ₁₂ - 0.002, K ₃ - 0.05 g, B _s - 0.03 g), food ingredients (oat flour, buckwheat flour, corn flour, soy isolate, soybean meal, food flavoring (GOST R 52464-2005 - Flavoring and food additives . Flavorings s), tuber of Jerusalem artichoke powder, roots of carrot powder), Trilon B, sodium metabisulfite, purified water at the following content of components, mass%:

Glucosamine salt 2,50 Dry willow extract 0.15 Vitamin complex "Vitam" 1.20 Trilon B 0.20 Sodium metabisulfite 0.30 Purified water 5.00 Food ingredients up to 100.00

As a glucosamine salt, the feed contains glucosamine hydrochloride, or sodium, or potassium, or calcium salt of glucosamine sulfate.

Glucosamine salt activates the synthesis of proteoglycans, hyaluronic, chondroitinsulfuric acids and other substances, which are the building material for articular membranes, intraarticular fluid and cartilage, enhances the production of cartilage matrix; normalizes calcium deposition in bone tissue; It has a moderate anti-inflammatory effect; with systematic use, it slows down the progression of degenerative processes in the joints, spine [An experimental study of the effect of glucosamine hydrochloride on metabolic and reparative processes in connective tissue structures / I.A. Zupanets, N.V. Bezdetko, N.V.Dedukh, I.A. Otrishko / / Experimental and clinical pharmacology. - 2002. - T. 65, No. 6. - S. 67-69].

Willow extract (TU 9199-006-33150849-2004) contains salicin phenol glycoside, which, under the action of the salicase enzyme, is hydrolytically split into aglycon-Saleginin and glucose. Saleginin is further oxidized to salicylic acid, which inhibits the synthesis of prostaglandins, thereby inhibiting inflammation [P. Kyosev. Full reference medicinal plants / Kjosev P.A. - M .: EKSMO-Press, 2001. - 992 p.].

Food components of the feed (oatmeal, buckwheat flour, corn flour, food flavoring, Jerusalem artichoke tubers powder, soy isolate, soybean meal, carrot root powder) are not only the basic components, but also give it a pleasant taste and smell. Oat, corn and buckwheat flour, soybean isolate, soybean meal, carrot and Jerusalem artichoke root powder are a source of proteins, polysaccharides, microelements [Chemical composition of food products. Directory. / Ed. I.M. Skurikhina and M.N. Volgareva - M .: Agropromizdat, 1987. - 360 pp.] And have a protective effect on the gastrointestinal mucosa. In addition, Jerusalem artichoke tubers powder has anticarcinogenic activity, antioxidant, anti-inflammatory and immunomodulating properties, helps cleanse the body of heavy metals and poisons, is a source of dietary fiber [Pilat TP, Ivanov A.A. Biologically active additives. - M .: Avvalon, 2002. - 710 p.].

Adding a vitamin complex to the base provides the composition with the necessary amount of vitamins.

Significant distinguishing features of the present invention are the increase in the anti-arthritic effect of the glucosamine salt due to the introduction of willow extract into the composition and the organoleptic properties of the feed, ensuring its voluntary intake by pets.

The amount of glucosamine salt per portion of a composition weighing 20 g (the amount of composition per 1 dose) was determined by calculation, taking into account literature data on effective therapeutic doses of glucosamine [Encyclopedia of drugs (Drug Register) - M .: LLC “RLS-2006”, 2006. - 1600 pp.] And the dose-conversion factor from person to dog [Guidelines for the experimental (preclinical) study of new pharmacological substances. - M., 2000. - 398 p.]. It was 0.5 g for a dog weighing 10 kg.

The amount of dry willow extract per serving of a composition weighing 20 g (amount of composition per 1 dose) was determined by calculation, taking into account the literature data on the recommended doses [Rational nutrition. Recommended levels of consumption of food and biologically active substances / Guidelines MP 2.3.1. 19150-04. - M., 2004.] and the dose conversion factor from person to dog [Guidelines for the experimental (preclinical) study of new pharmacological substances. - M., 2000. - 398 p.]. It was 0.03 g for a dog weighing 10 kg.

The amount of the vitamin complex per serving portion of a composition weighing 20 g (the amount of composition per 1 dose) was calculated in accordance with the recommendations of the Vidal Veterinarian reference book [Vidal Veterinarian Reference Book. Veterinary Medicines in Russia: A Guide. - M.: AstraPharmService, 2003/2004. - 480 p.]. It amounted to 0.12 g.

The technology of this composition is as follows: the following ingredients are sequentially loaded into the mixer (dried to a residual moisture content of 2%, with a particle size of not more than 0.5 mm): food flavoring, vitamin complex, dry willow extract, Trilon B, sodium metabisulfite, glucosamine salt food ingredients. The mixture is stirred until a homogeneous mass and moistened with purified water to obtain a plastic mass. The moistened mass is passed through a granulator. The obtained granules are dried in an oven at a temperature of 35-40 ° C to a residual moisture content of 5%.

Examples of the proposed feed.

Example 1. Food ingredients: food flavoring, vitamin premix, dry willow extract, glucosamine sulfate, carrot root powder, Jerusalem artichoke tubers powder, buckwheat flour, oatmeal flour were previously dried to a residual moisture content of not more than 2%, crushed to particles with a particle size of not more than 0.5 mm and sieved through a sieve. The horizontal multi-blade mixer was loaded alternately: food flavoring - 0.50 kg, vitamin premix - 0.12 kg, dry willow extract - 0.15 kg, Trilon B - 0.20 kg, sodium metabisulfite - 0.30 kg, glucosamine sulfate - 2.50 kg, carrot root crop powder - 4.0 kg, Jerusalem artichoke tubers powder - 25.5 kg, buckwheat flour - 30.00 kg, oatmeal - 31.73 kg (95.00 kg in total). The mixture was stirred until a color uniform mass was obtained. The dry mixture was moistened with purified water to obtain a plastic mass with a ratio of components 1: 0.5; those. per 100 kg of dry weight spent 50.0 kg of purified water. The wetted mass was passed through a granulator. The obtained granules were dried in an oven at a temperature of 35-40 ° C to a residual moisture content of 5%.

Example 2. Food ingredients: food flavoring, vitamin premix, dry willow extract, glucosamine sulfate, carrot root powder, soybean meal, soybean isolate, buckwheat flour, oatmeal were previously dried to a residual moisture content of not more than 2%, crushed to particles with a particle size not more than 0.5 mm and sieved through a sieve. The horizontal multi-blade mixer was loaded alternately: food flavoring - 0.50 kg, vitamin premix - 0.12 kg, dry willow extract - 0.15 kg, Trilon B - 0.20 kg, sodium metabisulfite - 0.30 kg, glucosamine sulfate - 2.50 kg, carrot root crop powder - 4.0 kg, soybean isolate - 16.81 kg, soybean meal - 34.70 kg, buckwheat flour - 17.86 kg, oatmeal - 17.86 kg (95, 00 kg). The mixture was stirred until a color uniform mass was obtained. The dry mixture was moistened with purified water to obtain a plastic mass with a ratio of components 1: 0.5; those. per 100 kg of dry weight spent 50.0 kg of purified water. The wetted mass was passed through a granulator. The obtained granules were dried in an oven at a temperature of 35-40 ° C to a residual moisture content of 5%.

Example 3. Food ingredients: food flavoring, vitamin premix, dry willow extract, glucosamine sulfate, carrot root powder, soybean meal, soybean isolate, corn flour were pre-dried to a residual moisture content of not more than 2%, crushed to particles with a particle size of not more than 0 5 mm and sieved through a sieve. The horizontal multi-blade mixer was loaded alternately: food flavoring - 0.50 kg, vitamin premix - 0.12 kg, dry willow extract - 0.15 kg, Trilon B - 0.20 kg, sodium metabisulfite - 0.30 kg, glucosamine sulfate - 2.50 kg, carrot root crop powder - 4.0 kg, soybean isolate - 16.81 kg, soybean meal - 34.70 kg, cornmeal - 35.72 kg (total 95.00 kg). The mixture was stirred until a color uniform mass was obtained. The dry mixture was moistened with purified water to obtain a plastic mass with a ratio of components 1: 0.5; those. per 100 kg of dry weight spent 50.0 kg of purified water. The wetted mass was passed through a granulator. The obtained granules were dried in an oven at a temperature of 35-40 ° C to a residual moisture content of 5%.

Example 4. Feed ingredients: food flavoring, vitamin premix, dry willow extract, glucosamine sulfate, carrot root powder, soybean meal, soybean isolate, oatmeal were previously dried to a residual moisture content of not more than 2%, crushed to particles with a particle size of not more than 0 5 mm and sieved through a sieve. The horizontal multi-blade mixer was loaded alternately: food flavoring - 0.50 kg, vitamin premix - 0.12 kg, dry willow extract - 0.15 kg, Trilon B - 0.20 kg, sodium metabisulfite - 0.30 kg, glucosamine sulfate - 2.50 kg, carrot root crop powder - 4.0 kg, soybean isolate - 16.81 kg, soybean meal - 34.70 kg, oatmeal - 35.72 kg (95.00 kg in total). The mixture was stirred until a color uniform mass was obtained. The dry mixture was moistened with purified water to obtain a plastic mass with a ratio of components 1: 0.5; those. per 100 kg of dry weight spent 50.0 kg of purified water. The wetted mass was passed through a granulator. The obtained granules were dried in an oven at a temperature of 35-40 ° C to a residual moisture content of 5%.

The anti-arthritic activity of this food was studied in dogs of the same age group (2-3 years) without signs of osteoarticular pathology. For the study we used healthy sexually mature dogs of males weighing 10-15 kg that were quarantined. Four groups were formed by random sampling. In the control series of experiments, the animals were injected with a feed base (its food ingredients) that did not contain active substances (group 1). The anti-arthritic activity of the proposed feed compositions was studied at a dose of 50 mg / kg (in terms of glucosamine), i.e. dogs weighing 10 kg received 20.0 g of feed (i.e. 2 g / kg of body weight) (group 2). The choice of this dose was determined by calculation, taking into account the literature on effective therapeutic doses of glucosamine [Encyclopedia of drugs (Drug Register) - M .: LLC “RLS-2006”, 2006. - 1600 p.]. The comparison drug — glucosamine hydrochloride tablets — was studied at a dose of 50 mg / kg (in terms of glucosamine), i.e. a dog weighing 10 kg was administered 1 tablet (group 3). Imported food with glucosamine of industrial production [Energy 4800, Royal Canin, France - TU 9296-001-59349152-04] was introduced in the amount recommended by the food manufacturer - 10 g / kg (group 4). Glucosamine hydrochloride tablets were placed on the root of the tongue of the animals before eating (for 10-15 minutes). The animals were fed voluntarily 10-15 minutes before receiving the main portion of food.

The food of the proposed composition, glucosamine hydrochloride tablets was prescribed as monotherapy for 30 days at the rate of 1 tablet per 10 kg of animal weight 2 times a day, industrial food was also prescribed for 30 days in the amounts recommended by the food manufacturer.

The anti-arthritic effect of the objects of study was studied on a model of post-traumatic arthrosis in dogs. Post-traumatic arthrosis developed in the hip joint after applying a standard defect in the form of a round hole with a diameter of 2 mm, penetrating through the articular cartilage into the subchondrial bone [I. Zupanets Experimental modeling of post-traumatic osteoarthritis of the hip joint / Zupanets I.A., Dragovoz S.M., Bezdetko N.V., Dedukh N.V. // Assessment of the pharmacological activity of chemical compounds: principles and approaches: Abstracts. doc. All-Union. scientific conf. - M., 1989 .-- Part 2. - S.132].

The clinical manifestations of osteoarthrosis (OA) in dogs were evaluated according to the following criteria: joint mobility, joint pain, the presence of a local inflammatory reaction.

An analysis of joint mobility in the control group of animals and in the groups that were fed the food of the proposed composition, the comparison drug — glucosamine hydrochloride tablets, and food from industrial production indicates that 24 hours after injuring the femoral head, joint mobility in all the animals studied is significantly impaired (Table 1).

In table 1, the data were evaluated as follows:

0 - no change in mobility;

- "-" - reduction of joint mobility;

- 1 - minimal decrease in joint mobility (in animals the lameness is weakly expressed);

- 2 - unexpressed limitation of joint mobility (animal is lame);

- 3 - restriction of joint mobility (the animal is significantly lame, but relies on a damaged limb);

- 4 - significant suppression of joint mobility (the animal does not rely on a damaged limb);

- 5 - joint mobility is practically absent (the animal practically does not get up).

An analysis of the data of the study indicates that the course application of the feed of this composition in OA is more effective than the comparison drug - glucosamine hydrochloride tablets and food of industrial production, helps to increase the mobility of the damaged joint 7 and 30 days after modeling the pathology (table 1). When studying the effect of the food of the proposed composition on joint pain during experimental OA, it was found that the use of anti-arthritic food of the proposed composition already 7 days after the start of treatment leads to a statistically significant decrease in algesia, and by the end of the experiment, pain in animals was practically not observed. The use of glucosamine hydrochloride tablets, feed of industrial production had a less pronounced effect on the studied indicator than the feed of the studied composition (table 2).

In table 2, the data were evaluated as follows:

0 - lack of pain;

- 1 - minimal pain (the animal makes an attempt to move away on palpation, but suffers);

- 2 - soreness of medium strength (the animal is removed by palpation);

- 3 - significant soreness (the animal abruptly withdraws the limb upon palpation);

- 4 - very pronounced soreness (it is not possible to palpate a diseased limb due to severe soreness).

As a result of the studies, it was found that the use of food of the proposed composition in the treatment of osteoarthritis in dogs leads to a significant improvement in the condition of animals, stopping the clinical manifestations of the disease (improving joint mobility, relieving pain, eliminating inflammatory joint edema).

Thus, the study allows us to recommend the use of feed of the proposed composition in veterinary practice for the treatment of osteoarthritis in dogs, which is more effective than the comparison drug - glucosamine hydrochloride tablets and industrial feed.

Table 1 Change in dog joint mobility after modeling osteoarthritis Duration of observation Groups of animals Control (group 1) Composition (group 2) Glucosamine hydrochloride (group 3) Food for industrial production (group 4) 24 hours -4.0 ± 0.33 -3.9 ± 0.25 -3.8 ± 0.33 -4.2 ± 0.20 7 days -4.4 ± 0.13 -2.2 ± 0.21 ^* -3.1 ± 0.20 ^* -3.3 ± 0.25 30 days -3.2 ± 0.22 -0.9 ± 0.24 ^* -1.4 ± 0.10 ^* -2.2 ± 0.17 ^* Note: ^* - statistically significant changes (P <0.05) relative to control animals are indicated.

table 2 Dog joint pain after modeling osteoarthritis Duration of observation Groups of animals Control (group 1) Composition (group 2) Glucosamine hydrochloride (group 3) Food for industrial production (group 4) 24 hours 3.8 ± 0.11 3.6 ± 0.22 3.4 ± 0.22 3.7 ± 0.22 7 days 3.6 ± 0.11 2.1 ± 0.18 ^* 2.9 ± 0.22 ^* 3.0 ± 0.21 30 days 3.0 ± 0.26 0.7 ± 0.13 ^* 1.2 ± 0.22 ^* 2.0 ± 0.25 ^* Note: ^* - statistically significant changes (P <0.05) relative to control animals are indicated.

Claims (2)

1. Food for the prevention of arthrosis in animals, characterized in that it contains glucosamine salt, dry willow extract, Vitam vitamin complex, Trilon B, sodium metabisulfite, purified water and food ingredients in the following components, wt.%:
Glucosamine salt 2,50 Dry willow extract 0.15 Vitamin complex "Vitam" 1.20 Trilon B 0.20 Sodium metabisulfite 0.30 Purified water 5.00 Food ingredients Up to 100.00 2. The food according to claim 1, characterized in that glucosamine hydrochloride, or sodium, or potassium, or calcium salt of glucosamine sulfate is used as the glucosamine salt.