RU2360246C1 - Way of diagnostics of parasitogenic disease - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention concerns medicine area, more precisely, to diagnostics of parasitogenic diseases on the basis of the analysis of blood and can be applied in clinical practice. The way of diagnostics of the parasitogenic disease, including search and recognition of endoparasites and persistent microorganisms - originators of infectious and parasitogenic diseases at observation through a microscope of a native smear of blood of the patient, differing that 20-30 minutes prior to a blood sampling the patient accepts an antioxidatic preparation, and a smear of native blood through a microscope with augmentation more 1000x is observed.

EFFECT: rising of accuracy of diagnostics of blood protozoan diseases and reduction of time of diagnostics.

8 cl, 3 dwg, 2 tbl, 2 ex

Description

The invention relates to medicine, more specifically to the diagnosis of parasitic diseases based on a blood test, and can be used in clinical practice.

Despite the high sensitivity and specificity of some diagnostic methods, their diagnostic value is limited. Specific test systems for their determination have not yet been developed for many pathogens. For example, antibodies to Ascaris proteins can be detected 5-10 days after infection, and after 3 months they are no longer detected. Many persistent microorganisms in human blood have a protein similar to a human protein, and blood dyes with standard staining, which is used in medicine, do not emit them, and the shapes and sizes of these microorganisms are comparable to red blood cells, and this does not allow one to distinguish between standard and enlargement from others.

Known methods for diagnosing a parasitic disease, including the search and recognition of endoparasites and persistent microorganisms - pathogens of infectious and parasitic diseases when observed through a microscope of a native blood smear of a patient, RF patent No. 2123682 dated 12/20/1998 and RF patent No. 21218565 dated 12/10/2003.

The disadvantages of the method described in the patent of the Russian Federation No. 2123682 dated 12/20/1998 is the need for preliminary preparation (staining) of blood intended for analysis, and the inability to distinguish persistent microorganisms similar in size to red blood cells.

The disadvantages of the method described in the patent of the Russian Federation No. 2218565 dated 12/10/2003, is also the need for preliminary (after its collection, but before analysis) preparation of blood (blood is centrifuged). In addition, any blood treatment after its collection leads to an increase in the time interval for the analysis and increases the risk of uncontrolled contamination of the blood and distortion of the results of the analysis.

The technical results of the invention are to increase the accuracy of the diagnosis of blood parasitic diseases and reduce the time of diagnosis.

These technical results are achieved in a method for diagnosing a parasitic disease, including the search for and recognition of endoparasites and persistent microorganisms - pathogens of infectious and parasitic diseases when a patient’s native blood smear is examined through a microscope, while the patient takes an antioxidant preparation before blood sampling, which leads to an increase in the concentration of endoparasites and persistent microorganisms in the blood 20-40 minutes after taking an antioxidant drug, and a native smear is observed blood immediately after its collection through a microscope with an increase of more than 1000x, which allows to detect and recognize parasitic forms.

It is thanks to a significant increase in the concentration of microorganisms in the blood that the use of a microscope with a large increase, which allows identifying parasitic forms, becomes effective. It is known that the angle of the field of view of the optical system is inversely proportional to the angular increase. Therefore, the use of a microscope with a large increase leads to a narrowing of the field of view of the microscope objective and, accordingly, to a decrease in the probability of detecting microorganisms. Therefore, it is so important to increase the concentration of microorganisms in the blood. In addition, increasing the concentration of microorganisms in the blood prior to its collection allows you to abandon the tricks to increase the concentration of microorganisms after blood sampling. This, in turn, makes it possible to study a blood smear immediately (directly) after its collection, which eliminates the possibility of any contamination of the blood.

Additionally, before the patient takes the antioxidant drug or before the expiration of 20 minutes after it is taken, they can take blood from the patient and observe a smear of native blood through a microscope with an increase from 1000x to 1500x, and compare the results of the search and recognition of endoparasites and persistent microorganisms with the results obtained when observing a smear of native blood, the sampling of which was carried out 20-40 minutes after the patient took the antioxidant drug.

In addition, it is possible to carry out a video recording of a native blood smear image obtained through a microscope.

Images of a native blood smear observed through a microscope can be displayed on a television screen.

You can observe a smear of native blood immediately after its collection through a microscope with an increase of more than 1400x.

Microhydrin can be used as an antioxidant drug.

As an antioxidant drug, you can use Hydrosel or Alka Mine.

Diagnosis in accordance with the claimed invention was carried out in 3000 patients for 3 years. The NICON ECLIPSE E200 microscope and observation methods in light and dark fields were used.

For diagnosis, native peripheral blood was used. Diagnostics was carried out, as a rule, in two stages. At the first stage, primary (preliminary) blood sampling was performed, which is examined in its native form.

In contrast to conventional laboratory methods for the study of blood smear, in the claimed method, the smear is not stained with dyes and is not fixed. The taken drop of blood is covered with a coverslip to obtain a monolayer smear. In the center of the projection of a blood smear, 1 drop of imersion oil is applied to the coverslip to more fully contact the objective lens and improve the optical ability of the microscope.

The NICON ECLIPSE E200 microscope was connected via an analog video camera to a computer monitor or TV, which provided parallel observation of the blood smear image in the microscope’s eyepiece (magnification from 1000x to 1500x) and on the monitor screen (linear increase is 3000). The resolution of the microscope provides a clear vision of the morphological structure of all parasitoforms present in the blood. And the output of the image to the monitor through the video block made it possible to increase the image on the monitor screen to 3000. For comparison, in standard laboratory practice, when examining blood smears, a 400x-900x microscope is used.

These technical capabilities made it possible to see in the blood smear the presence of not only blood cells (red blood cells, white blood cells and platelets), but also previously unidentified living objects, parasitoforms, that were independent of blood cells. Recognition of parasitic forms, possible with a sufficiently high magnification of the microscope (studies were carried out, as a rule, using a magnification of 1500x), allows you to diagnose parasitic disease.

The identification and research work on the differentiation of morphological forms of detected objects (parasitoforms) autonomous from blood cells in a native smear of peripheral blood made it possible to identify some of the most common parasitoforms in blood plasma. The morphological differences of the larval migration stages of helminths of representatives of all three systematic groups of helminths (nematodoses, cestodoses, trematodoses), rare local and imported larval helminth infections, protozoa (sporozoids, flagellates, +), various types of fungi (yeast-like, their mycelia and spore formation, bacterial infection of various forms and species (chlamydia, ureaplasma, mycoplasma, L-shaped bacteria, rod-shaped bacteria, etc.)

As a result of the study of groups of patients with chronic and oncological diseases of the gastrointestinal tract, cardiovascular and skin diseases, the following conclusion was made on the predominant localization of micro and macro parasitosis lesions in the following organs and systems (see table 1):

Table 1 Leather Respiratory system Muscle CNS Genitourinary Eye Amoeba dysentery + + Cryptosporidium + Hookworm + Roundworm + Opisthorch + Paragonim + + + Strongyloid + + + Trichinella + + + Toxocara + + + Fasciola + + Cysticercus + + + + Schistosomes + + + Pinworm + + Echinococcus + + + +

More than 70% of all detected commensals and parasites live in the gastrointestinal tract.

When diagnosing, we used a video recording system that allows us to document the observed image of a blood smear and significantly improve the quality of diagnosis, and, if necessary, attract highly qualified experts for an expert opinion on video recording.

At the second stage of diagnosis, the subject took an antioxidant drug, in particular, made on the basis of microcluster technology (antioxidants - Microhydrin, Hydrosel, Acidofilius, etc.) and, with some exposure, usually 20-40 minutes, peripheral blood was taken and repeated a blood smear test, which was also recorded on a videotape. As a result of taking the bioactive drug, there is a change in the physicochemical properties of blood plasma and formed elements, which leads to a significant increase (from 3 times to 35 times) of the concentration of microorganisms (exit from the depot of organ tissues) in a native smear of peripheral blood.

The most important is the second stage of diagnosis, which may be the only one. However, a comparison of the results of the first and second stages of diagnosis allows us to conclude on the stage of the parasitic disease and the degree of threat to the patient’s health.

The effectiveness of the use of antioxidants Microhydrin, Hydrosel, Acidophilus, etc. was especially evident in the diagnosis of parasitic disease in 20% of patients, when at the first stage of diagnosis parasitic forms were not detected, and at the second stage the presence of several types of parasitic forms was detected.

These antioxidants are modifiers of water (reduce the magnitude of its surface tension) and contribute to leaching of parasitoforms from tissue depots.

Thanks to the proposed diagnostic method, the causal factors of the biochemical shift of homeostasis for the development of homotoxicological staging in the development of chronic and oncological diseases were found and confirmed, and the conditions for the interruption of pathological links of the identified staging and the termination of the staging of the progression of this disease were studied.

It has been reliably shown that the use of the claimed diagnostic method and the use of the data obtained on its basis for conducting complex phased approaches in rehabilitation therapy ensures the restoration of homeostasis, the normalization of all biological parameters of the blood and the whole organism, leads to the release of humans from parasitic endofauna and flora of persistent microorganisms and macro parasites, which, in turn, leads to a persistent state of disease remission and a phased recovery of patients.

Table 2 below shows a comparative characteristic of the detection of parasites by enzyme-linked immunosorbent assay (ELISA), bacteriological method and the claimed method of scanning native peripheral blood.

table 2 The name of the pathogen Hemoscanning of native peripheral blood Bacteriological studies of blood smears according to Gram, R. Giemsa IFA Chlamydia 123 - 122 Mycoplasmosis 170 - 87 Ureaplasmosis 41 - fifteen Giardiasis 163 - 32 Toxoplasmosis 121 - 130 Trichomoniasis 362 - 41 Toxocariasis 82 - 12 Strongyloidosis 87 - - Hookworm infection 238 - - Dr. nematodes 122 - - Opisthorchiasis 48 - 21 Mycoses 362 - - Intracellular damage to blood cells: fungal, bacterial, helminth 178

Example 1

Cancer patient K., 32 years old, who was admitted for a diagnostic examination to the Department of Restorative Medicine on December 18, 2006 in serious condition, complaints of general weakness, lack of appetite, loss of interest in life, weight loss of 27 kg since 2002, periodic nausea and vomiting . The patient has difficulty moving, a state of cachexia. From the anamnesis: in 2003, the Cancer Research Institute of Moscow was diagnosed with Cancer rectum T2N0M0. He refused surgery and chemotherapy. He continued to work as an electrician until 2006. In March, the patient was admitted to City Hospital No. 62 for acute paraproctitis. Bred colostomy.

From the anamnesis of vitae: lives in a wooden house in a village near Moscow with his wife, mother and grandmother. There is a farm of pets. The maternal grandmother and mother were diagnosed with sigmoid colon cancer, sigmoid colon sectoral resection - in both. Currently, the mother feels satisfactory, the grandmother is not alive. In May 2005, the patient’s wife, K., became pregnant from him and, at the 29th week of pregnancy, the pregnancy was terminated due to intrauterine death, the fetus was found to have congenital malformations.

30 minutes before a blood test by scanning with a NICON ECLIPCE E200 microscope in a light and dark field before it, the patient took antioxidant drugs of the nanocluster series - aqueous solutions:

Microhydrin 1 capsule per 200 ml of water, Alka Mine 1 powder per 200 ml of water, Hydrosel - 10 drops of solution in each glass with antioxidants. After 30 min, peripheral blood was taken and the following picture was visualized on the monitor screen.

In the blood revealed: severe iron deficiency anemia, poikilocytosis, echinocytosis, 3 tbsp. plasma toxicity (fungal spores, bacteremia, phage and algal forms), intracellular damage to red blood cells (streptococcal and fungal infections) - 1 p / sp. 15–25 infected red blood cells, eosinophilia, 3 stage immunosuppression, multiple lamblia cysts, imperfect carnivorous fungi at the stages of aggressive capture (ejection of hunting rings) and reproduction, symbiotic tissue formation of diatom algae and imperfect carnivorous fungi in the form of translucent whitish tissues unevenly distributed migration larval forms of nematodes (ascariasis, hookworm, toxocariasis and other forms of rare unexplained morphology) - 3-5 parasitoforms in each field of view, tr hematode (schistosomatosis and other forms of rare flukes) - 7-12 parasitoforms in 1 smear, multiple cystic and autonomic flagellate trichomonads in each field of view - 15-25 forms.

In each field of view there are 15-25 parasitoforms and 15-25 damaged red blood cells (N of the quantitative composition of red blood cells is 35-45 in each field of view).

After the courses of deworming, the patient experienced the release of various types of helminths and then an objective improvement in the general condition and parameters of laboratory blood tests. Therefore, in the remaining family members, in view of the same clinical diagnoses of the whole family - colon cancer - the etiology of the disease can be interpreted as infectious-parasitic and hematoplacental infection with helminthiasis, bacterial-protozoal and microplasma infection transmitted by the infected grandmother of the patient's mother, and the mother to the son, can be interpreted.

Example 2

Oncological patient M., 45 years old, who was admitted for a diagnostic examination in serious condition, complaints of general weakness, periodic nausea and vomiting, cachexia state.

A blood test by scanning with a NICON ECLIPCE E200 microscope in a light and dark field is performed on an empty stomach. 1 drop of peripheral blood from a finger is placed on a glass slide. The taken drop of blood is covered with a coverslip to obtain a monolayer smear. In the center of the projection of a blood smear, 1 drop of imersion oil is applied to the coverslip to more fully contact the objective lens and improve the optical resolution of the microscope.

When scanning the native peripheral blood of patient M. found:

1 study:

Severe iron deficiency anemia, poikilocytosis, echinocytosis, 2 tbsp. Plasma toxicity (fungal spores, bacteremia, phage and algal forms), intracellular damage to red blood cells (streptococcal and fungal infection) - in 1 p / sp. 5-10 infected red blood cells, eosinophilia, immunosuppression of 3 tbsp., Imperfect carnivorous fungi at the stages of aggressive capture (ejection of hunting rings) and reproduction, single spores of diatoms, migratory larval forms of nematodes (ascariasis, hookworm infection) - 2-3 parasitoforms 15 fields of view, trematodes (schistosomatosis and other forms of rare helminths of flukes) - 3-6 parasitoforms in 1 monolayer smear, flagellate forms of Trichomonas - 5-6 in each field of view.

In each field of view, 5-10 parasitoforms and 5-10 damaged red blood cells (N of the quantitative composition of red blood cells 35-45 in each field of view).

2 research:

30 minutes before it was held, patient M. took antioxidant drugs of the nanocluster (microcluster) series, namely aqueous solutions: Microhydrin 1 capsule per 200 ml of water, Alka Mine 1 powder per 200 ml of water, Hydrosel - 10 drops of solution in each glass with antioxidants. After 30 min, peripheral blood was retaken and the following picture was visualized on the monitor screen.

Against the background of an improvement in the general microcirculatory hemodynamics in the blood, the following was revealed: severe iron deficiency anemia, poikilocytosis, echinocytosis, 3 tbsp. plasma toxicity (fungal spores, bacteremia, phage and algal forms), intracellular damage to red blood cells (streptococcal and fungal infections) - 1 p / sp. 15–25 infected red blood cells, eosinophilia, 3 stage immunosuppression, multiple lamblia cysts, imperfect carnivorous fungi at the stages of aggressive capture (ejection of hunting rings) and reproduction, symbiotic tissue formation of diatom algae and imperfect carnivorous fungi in the form of translucent whitish tissues unevenly distributed migration larval forms of nematodes (ascariasis, hookworm, toxocariasis and other forms of rare unexplained morphology) - 3-5 parasitoforms in each field of view, tr hematode (schistosomatosis and other forms of rare flukes) 7-12 parasitoforms in 1 smear, multiple cystic and autonomic flagellate forms of trichomonads in each field of view 15-25 forms.

In each field of view, 15-30 parasitoforms and 15-25 damaged red blood cells (N of the quantitative composition of red blood cells 35-45 in each field of view).

After a cyclic phased therapeutic and rehabilitation course, the patient felt an objective improvement in general condition.

On the control scan after 2 months of treatment, the plasma purity parameters improved (in 1 p / sp. Of infected red blood cells there were 3-6, blood cells in relation to the number of parasitoforms became 2 times larger (5-10 parasitoforms and 25-35 Er), the number of segmented neutrophils increased 1.5 times compared with the initial examination.

In the presented drawings (Fig. 1), the peripheral native blood of an oncological patient M., aged 45, was scanned using a Nicon Eclipse E200 microscope and scanning microscopy images (Figs. 2, 3).

Figure 1 presents multiple larval forms of helminths (nematodes, cestodes and trematodes), fungi of various species during breeding periods, iron deficiency anemia.

Figure 2 presents a snapshot of scanning electron microscopy of symbiotic tissue, in the construction of which metamers are only partially used. It is characteristic of chronic diseases of the last stages.

Figure 3 presents a photograph of scanning electron microscopy of various forms of tissue from metameres. It is characteristic for cancer of the second and third stages.

Obviously, the most important is the second stage of diagnosis, which may be the only one. However, a comparison of the results of the first and second stages of diagnosis allows us to conclude on the stage of the parasitic disease and the degree of threat to the patient’s health.

These cases clearly show the presence of infectious-parasitic continuity of generations and draw a parallel between parasitoses and the development of cancer.

Research statistics indicate that each person has a carriage of helminths of single species and mixed helminth infestation, protozoa, fungi, pathogenic bacteria and persistent microorganisms. Only in different titration amounts and quality. And when favorable conditions arise for them, which the author described above, there is a violation of the balance of biocenoses in the human body and their mass reproduction. The histopathological and morphological links of the pathogenesis of chronic and oncological diseases are included.

Blood is a mirror of life and a mirror of human diseases. After the water antioxidants due to the release of the organs of associative microbiocenoses “hidden” in the depot, we see their quantitative and species increase in each field of view of the blood fields under study (700-900 fields on a 19-inch screen of the monitor are seen in 1 study).

The concentration of parasitoses after water antioxidants increases from 3-35 times in all fields of view.

Claims (8)

1. A method for diagnosing a parasitic disease, including the search and recognition of endoparasites and persistent microorganisms - pathogens of infectious and parasitic diseases when observed through a microscope of a patient's native blood smear, characterized in that the patient takes an antioxidant drug 20-30 minutes before blood sampling, and a smear is observed native blood through a microscope with an increase of more than 1000x. 2. The method according to claim 1, characterized in that in addition to taking the antioxidant preparation, the patient is pre-sampled from the patient and a smear of native blood is observed through a microscope with an increase of more than 1000x, and the results of the search and recognition of endoparasites and persistent microorganisms are compared with the results obtained when observing a smear of native blood, the sampling of which was carried out 20 to 40 minutes after the patient took the antioxidant drug. 3. The method according to claim 1, characterized in that it additionally carry out a video recording of a native blood smear obtained through a microscope. 4. The method according to claim 1, characterized in that the images of the native blood smear observed through a microscope are displayed on a television screen. 5. The method according to claim 1, characterized in that a smear of native blood is observed immediately after its collection through a microscope with an increase of more than 1400x. 6. The method according to claim 1, characterized in that Microhydrin is used as an antioxidant preparation. 7. The method according to claim 1, characterized in that Hydrosel is used as an antioxidant preparation. 8. The method according to claim 1, characterized in that Alka Mine is used as an antioxidant preparation.

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